                                       SEQUENCE LISTING
              
              <110>  ROQUETTE FRERES
               
              <120>  Improved variant of D-psicose 3-epimerase and uses thereof
              
              <130>  B1628PC
              
              <160>  20    
              
              <170>  PatentIn version 3.3
              
              <210>  1
              <211>  879
              <212>  DNA
              <213>  Clostridium cellulolyticum
              
              
              <220>
              <221>  CDS
              <222>  (1)..(879)
              
              <400>  1
              atg aaa cat ggt ata tac tac gca tat tgg gaa caa gaa tgg gaa gct       48
              Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala         
              1               5                   10                  15              
              
              gat tac aaa tac tat att gag aag gtt gca aag ctt ggt ttt gat att       96
              Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile         
                          20                  25                  30                  
              
              cta gag att gca gct tca ccg cta cct ttt tac agt gac att cag att      144
              Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Ile Gln Ile         
                      35                  40                  45                      
              
              aat gag ctc aag gca tgt gcc cat ggc aat gga att aca ctt acg gta      192
              Asn Glu Leu Lys Ala Cys Ala His Gly Asn Gly Ile Thr Leu Thr Val         
                  50                  55                  60                          
              
              ggc cat ggg cct agt gca gaa caa aac ctg tct tct ccc gac ccc gat      240
              Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Asp         
              65                  70                  75                  80          
              
              att cgc aaa aat gct aaa gct ttt tat acc gat tta ctc aaa cga ctt      288
              Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu         
                              85                  90                  95              
              
              tac aag ctg gat gta cat ttg ata ggt ggg gct tta tat tct tat tgg      336
              Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp         
                          100                 105                 110                 
              
              ccg ata gat tac aca aag aca att gat aaa aaa ggc gat tgg gaa cgc      384
              Pro Ile Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg         
                      115                 120                 125                     
              
              agc gtt gaa agt gtt cga gaa gtt gct aag gtg gcc gaa gcc tgt gga      432
              Ser Val Glu Ser Val Arg Glu Val Ala Lys Val Ala Glu Ala Cys Gly         
                  130                 135                 140                         
              
              gtg gat ttc tgc cta gag gtt ctt aat aga ttt gag aat tat tta att      480
              Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile         
              145                 150                 155                 160         
              
              aac aca gca caa gag ggt gta gat ttt gta aaa cag gtt gac cat aac      528
              Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Asp His Asn         
                              165                 170                 175             
              
              aat gta aag gta atg ctt gat acc ttc cac atg aat att gag gaa gat      576
              Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp         
                          180                 185                 190                 
              
              agt atc gga ggt gca atc agg act gcg ggc tct tac ttg gga cat tta      624
              Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu         
                      195                 200                 205                     
              
              cac act ggc gaa tgt aat cgt aaa gtt ccc ggc aga gga aga att cca      672
              His Thr Gly Glu Cys Asn Arg Lys Val Pro Gly Arg Gly Arg Ile Pro         
                  210                 215                 220                         
              
              tgg gta gaa att ggt gag gct ctt gct gac ata ggt tat aac ggt agt      720
              Trp Val Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser         
              225                 230                 235                 240         
              
              gtt gtt atg gaa cct ttt gtt aga atg ggc gga act gtc gga tct aat      768
              Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn         
                              245                 250                 255             
              
              att aag gtt tgg cgt gac att agt aac ggt gca gat gag aaa atg ctg      816
              Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Lys Met Leu         
                          260                 265                 270                 
              
              gat aga gaa gca cag gcc gca ctt gat ttc tcc aga tat gta tta gaa      864
              Asp Arg Glu Ala Gln Ala Ala Leu Asp Phe Ser Arg Tyr Val Leu Glu         
                      275                 280                 285                     
              
              tgt cat aaa cac tcc                                                  879
              Cys His Lys His Ser                                                     
                  290                                                                 
              
              
              <210>  2
              <211>  293
              <212>  PRT
              <213>  Clostridium cellulolyticum
              
              <400>  2
              
              Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala 
              1               5                   10                  15      
              
              
              Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
                          20                  25                  30          
              
              
              Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Ile Gln Ile 
                      35                  40                  45              
              
              
              Asn Glu Leu Lys Ala Cys Ala His Gly Asn Gly Ile Thr Leu Thr Val 
                  50                  55                  60                  
              
              
              Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Asp 
              65                  70                  75                  80  
              
              
              Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu 
                              85                  90                  95      
              
              
              Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp 
                          100                 105                 110         
              
              
              Pro Ile Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg 
                      115                 120                 125             
              
              
              Ser Val Glu Ser Val Arg Glu Val Ala Lys Val Ala Glu Ala Cys Gly 
                  130                 135                 140                 
              
              
              Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile 
              145                 150                 155                 160 
              
              
              Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Asp His Asn 
                              165                 170                 175     
              
              
              Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
                          180                 185                 190         
              
              
              Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu 
                      195                 200                 205             
              
              
              His Thr Gly Glu Cys Asn Arg Lys Val Pro Gly Arg Gly Arg Ile Pro 
                  210                 215                 220                 
              
              
              Trp Val Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser 
              225                 230                 235                 240 
              
              
              Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn 
                              245                 250                 255     
              
              
              Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Lys Met Leu 
       
                          260                 265                 270         
              
              
              Asp Arg Glu Ala Gln Ala Ala Leu Asp Phe Ser Arg Tyr Val Leu Glu 
                      275                 280                 285             
              
              
              Cys His Lys His Ser 
                  290             
              
              
              <210>  3
              <211>  879
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  CC-DPEase variant
              
              
              <220>
              <221>  CDS
              <222>  (1)..(879)
              
              <400>  3
              atg aaa cat ggt ata tac tac gca tat tgg gaa caa gaa tgg gaa gct       48
              Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala         
              1               5                   10                  15              
              
              gat tac aaa tac tat att gag aag gtt gca aag ctt ggt ttt gat att       96
              Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile         
                          20                  25                  30                  
              
              cta gag att gca gct tca ccg cta cct ttt tac agt gac att cag att      144
              Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Ile Gln Ile         
                      35                  40                  45                      
              
              aat gag ctc aag gca tgt gcc cat ggc aat gga att aca ctt acg gta      192
              Asn Glu Leu Lys Ala Cys Ala His Gly Asn Gly Ile Thr Leu Thr Val         
                  50                  55                  60                          
              
              ggc cat ggg cct agt gca gaa caa aac ctg tct tct ccc gac ccc gat      240
              Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Asp         
              65                  70                  75                  80          
              
              att cgc aaa aat gct aaa gct ttt tat acc gat tta ctc aaa cga ctt      288
              Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu         
                              85                  90                  95              
              
              tac aag ctg gat gta cat ttg ata ggt ggg gct tta tat tct tat tgg      336
              Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp         
                          100                 105                 110                 
              
              ccg ata gat tac aca aag aca att gat aaa aaa ggc gat tgg gaa cgc      384
              Pro Ile Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg         
                      115                 120                 125                     
              
              agc gtt gaa agt gtt cga gaa gtt gct aag gtg gcc gaa gcc tgt gga      432
              Ser Val Glu Ser Val Arg Glu Val Ala Lys Val Ala Glu Ala Cys Gly         
                  130                 135                 140                         
              
              gtg gat ttc tgc cta gag gtt ctt aat aga ttt gag aat tat tta att      480
              Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile         
              145                 150                 155                 160         
              
              aac aca gca caa gag ggt gta gat ttt gta aaa cag gtt gac cat aac      528
              Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Asp His Asn         
                              165                 170                 175             
              
              aat gta aag gta atg ctt gat acc ttc cac atg aat att gag gaa gat      576
              Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp         
                          180                 185                 190                 
              
              agt atc gga ggt gca atc agg act gcg ggc tct tac ttg gga cat tta      624
              Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu         
                      195                 200                 205                     
              
              cac act agc gaa tgt aat cgt aaa gtt ccc ggc aga gga aga att cca      672
              His Thr Ser Glu Cys Asn Arg Lys Val Pro Gly Arg Gly Arg Ile Pro         
                  210                 215                 220                         
              
              tgg gta gaa att ggt gag gct ctt gct gac ata ggt tat aac ggt agt      720
              Trp Val Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser         
              225                 230                 235                 240         
              
              gtt gtt atg gaa cct ttt gtt aga atg ggc gga act gtc gga tct aat      768
              Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn         
                              245                 250                 255             
              
              att aag gtt tgg cgt gac att agt aac ggt gca gat gag aaa atg ctg      816
              Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Lys Met Leu         
                          260                 265                 270                 
              
              gat aga gaa gca cag gcc gca ctt gat ttc tcc aga tat gta tta gaa      864
              Asp Arg Glu Ala Gln Ala Ala Leu Asp Phe Ser Arg Tyr Val Leu Glu         
                      275                 280                 285                     
              
              tgt cat aaa cac tcc                                                  879
              Cys His Lys His Ser                                                     
                  290                                                                 
              
              
              <210>  4
              <211>  293
              <212>  PRT
              <213>  artificial sequence
              
              <220>
              <223>  Synthetic Construct
              
              <400>  4
              
              Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala 
              1               5                   10                  15      

              
              
              Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
                          20                  25                  30          
              
              
              Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Ile Gln Ile 
                      35                  40                  45              
              
              
              Asn Glu Leu Lys Ala Cys Ala His Gly Asn Gly Ile Thr Leu Thr Val 
                  50                  55                  60                  
              
              
              Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Asp 
              65                  70                  75                  80  
              
              
              Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu 
                              85                  90                  95      
              
              
              Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp 
                          100                 105                 110         
              
              
              Pro Ile Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg 
                      115                 120                 125             
              
              
              Ser Val Glu Ser Val Arg Glu Val Ala Lys Val Ala Glu Ala Cys Gly 
                  130                 135                 140                 
              
              
              Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile 
              145                 150                 155                 160 
              
              
              Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Asp His Asn 
                              165                 170                 175     
              
              
              Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
                          180                 185                 190         
              
              
              Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu 
                      195                 200                 205             
              
              
              His Thr Ser Glu Cys Asn Arg Lys Val Pro Gly Arg Gly Arg Ile Pro 
                  210                 215                 220                 
              
              
              Trp Val Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser 
              225                 230                 235                 240 
              
              
              Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn 
                              245                 250                 255     
              
              
              Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Lys Met Leu 
                          260                 265                 270         
              
              
              Asp Arg Glu Ala Gln Ala Ala Leu Asp Phe Ser Arg Tyr Val Leu Glu 
                      275                 280                 285             
              
              
              Cys His Lys His Ser 
                  290             
              
              
              <210>  5
              <211>  292
              <212>  PRT
              <213>  Clostridium sp.
              
              <400>  5
              
              Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala 
              1               5                   10                  15      
              
              
              Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
                          20                  25                  30          
              
              
              Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Asn Gln Ile 
                      35                  40                  45              
              
              
              Asn Glu Leu Lys Ala Cys Ala Arg Gly Asn Gly Ile Thr Leu Thr Val 
                  50                  55                  60                  
              
              
              Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Tyr 
              65                  70                  75                  80  
              
              
              Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu 
                              85                  90                  95      
              
              
              Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Ile Tyr Ser Tyr Trp 
                          100                 105                 110         
              
              
              Pro Val Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg 
                      115                 120                 125             
              
              
              Ser Val Glu Ser Val Arg Glu Val Ala Gln Val Ala Glu Ala Cys Gly 
                  130                 135                 140                 
              
              
              Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile 
              145                 150                 155                 160 
              
              
              Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Gly His Asp 
                              165                 170                 175     
              
              
              Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
                          180                 185                 190         
              
              
              Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu 
                      195                 200                 205             
              
              
              His Thr Gly Glu Cys Asn Arg Lys Val Pro Gly Lys Gly Arg Ile Pro 
                  210                 215                 220                 
              
              
              Trp Ile Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser 
              225                 230                 235                 240 
              
              
              Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn 
                              245                 250                 255     
              
              
              Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Glu Lys Leu 
                          260                 265                 270         
              
              
              Asp Arg Glu Ala Gln Ala Ala Leu Asn Phe Ser Arg Tyr Val Leu Gly 
                      275                 280                 285             
              
              
              Asn Arg Lys Leu 
                  290         
              
              
              <210>  6
              <211>  289
              <212>  PRT
              <213>  Clostridium scindens
              
              <400>  6
              
              Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Ala Ala 
              1               5                   10                  15      
              
              
              Asp Tyr Lys Arg Tyr Val Glu Lys Ala Ala Lys Leu Gly Phe Asp Ile 
                          20                  25                  30          
              
              
              Leu Glu Val Gly Ala Ala Pro Leu Pro Asp Tyr Ser Ala Gln Glu Val 
                      35                  40                  45              
              
              
              Lys Glu Leu Lys Lys Cys Ala Asp Asp Asn Gly Ile Gln Leu Thr Ala 
                  50                  55                  60                  
              
              
              Gly Tyr Gly Pro Ala Phe Asn His Asn Met Gly Ser Ser Asp Pro Lys 
              65                  70                  75                  80  
              
              
              Ile Arg Glu Glu Ala Leu Gln Trp Tyr Lys Arg Leu Phe Glu Val Met 
                              85                  90                  95      
              
              
              Ala Gly Leu Asp Ile His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp 
                          100                 105                 110         
              
              
              Pro Val Asp Phe Ala Thr Ala Asn Lys Glu Glu Asp Trp Lys His Ser 
                      115                 120                 125             
              
              
              Val Glu Gly Met Gln Ile Leu Ala Pro Ile Ala Ser Gln Tyr Gly Ile 
                  130                 135                 140                 
              
              
              Asn Leu Gly Met Glu Val Leu Asn Arg Phe Glu Ser His Ile Leu Asn 
              145                 150                 155                 160 
              
              
              Thr Ser Glu Glu Gly Val Lys Phe Val Thr Glu Val Gly Met Asp Asn 
                              165                 170                 175     
              
              
              Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Ser Ser 
                          180                 185                 190         
              
              
              Ile Gly Asp Ala Ile Arg His Ala Gly Lys Leu Leu Gly His Phe His 
                      195                 200                 205             
              
              
              Thr Gly Glu Cys Asn Arg Met Val Pro Gly Lys Gly Arg Thr Pro Trp 
                  210                 215                 220                 
              
              
              Arg Glu Ile Gly Asp Ala Leu Arg Glu Ile Glu Tyr Asp Gly Thr Val 
              225                 230                 235                 240 
              
              
              Val Met Glu Pro Phe Val Arg Met Gly Gly Gln Val Gly Ser Asp Ile 
                              245                 250                 255     
              
              
              Lys Val Trp Arg Asp Ile Ser Lys Gly Ala Gly Glu Asp Arg Leu Asp 
                          260                 265                 270         
              
              
              Glu Asp Ala Arg Arg Ala Val Glu Phe Gln Arg Tyr Met Leu Glu Trp 
                      275                 280                 285             
              
              
              Lys 
                  
              
              
              <210>  7
              <211>  288
              <212>  PRT
              <213>  Agrobacterium tumefaciens
              
              <400>  7
              
              Met Lys His Gly Ile Tyr Tyr Ser Tyr Trp Glu His Glu Trp Ser Ala 
              1               5                   10                  15      
              
              
              Lys Phe Gly Pro Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
                          20                  25                  30          
              
              
              Ile Glu Val Ala Ala His His Ile Asn Glu Tyr Ser Asp Ala Glu Leu 
                      35                  40                  45              
              
              
              Ala Thr Ile Arg Lys Ser Ala Lys Asp Asn Gly Ile Ile Leu Thr Ala 
                  50                  55                  60                  
              
              
              Gly Ile Gly Pro Ser Lys Thr Lys Asn Leu Ser Ser Glu Asp Ala Ala 
              65                  70                  75                  80  
              
              
              Val Arg Ala Ala Gly Lys Ala Phe Phe Glu Arg Thr Leu Ser Asn Val 
                              85                  90                  95      
              
              
              Ala Lys Leu Asp Ile His Thr Ile Gly Gly Ala Leu His Ser Tyr Trp 
                          100                 105                 110         
              
              
              Pro Ile Asp Tyr Ser Gln Pro Val Asp Lys Ala Gly Asp Tyr Ala Arg 
                      115                 120                 125             
              
              
              Gly Val Glu Gly Ile Asn Gly Ile Ala Asp Phe Ala Asn Asp Leu Gly 
                  130                 135                 140                 
              
              

              Ile Asn Leu Cys Ile Glu Val Leu Asn Arg Phe Glu Asn His Val Leu 
              145                 150                 155                 160 
              
              
              Asn Thr Ala Ala Glu Gly Val Ala Phe Val Lys Asp Val Gly Lys Asn 
                              165                 170                 175     
              
              
              Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
                          180                 185                 190         
              
              
              Ser Phe Gly Asp Ala Ile Arg Thr Ala Gly Pro Leu Leu Gly His Phe 
                      195                 200                 205             
              
              
              His Thr Gly Glu Ser Asn Arg Arg Val Pro Gly Lys Gly Arg Met Pro 
                  210                 215                 220                 
              
              
              Trp His Glu Ile Gly Leu Ala Leu Arg Asp Ile Asn Tyr Thr Gly Ala 
              225                 230                 235                 240 
              
              
              Val Ile Met Glu Pro Phe Val Lys Thr Gly Gly Thr Ile Gly Ser Asp 
                              245                 250                 255     
              
              
              Ile Lys Val Trp Arg Asp Leu Ser Gly Gly Ala Asp Ile Ala Lys Met 
                          260                 265                 270         
              
              
              Asp Glu Asp Ala Arg Asn Ala Leu Ala Phe Ser Arg Phe Val Leu Gly 
                      275                 280                 285             
              
              
              <210>  8
              <211>  291
              <212>  PRT
              <213>  Ruminococcus sp.
              
              <400>  8
              
              Met Lys Tyr Gly Ile Tyr Tyr Ala Tyr Trp Glu Lys Glu Trp Asn Gly 
              1               5                   10                  15      
              
              
              Asp Tyr Lys Tyr Tyr Ile Asp Lys Ile Ser Lys Leu Gly Phe Asp Ile 
                          20                  25                  30          
              
              
              Leu Glu Ile Ser Cys Gly Ala Phe Ser Asp Tyr Tyr Thr Lys Asp Gln 
                      35                  40                  45              
              
              
              Glu Leu Ile Asp Ile Gly Lys Tyr Ala Lys Glu Lys Gly Val Thr Leu 
                  50                  55                  60                  
              
              
              Thr Ala Gly Tyr Gly Pro His Phe Asn Glu Ser Leu Ser Ser Ser Glu 
              65                  70                  75                  80  
              
              
              Pro Asn Thr Gln Lys Gln Ala Ile Ser Phe Trp Lys Glu Thr Leu Arg 
                              85                  90                  95      
              
              
              Lys Leu Lys Leu Met Asp Ile His Ile Val Gly Gly Ala Leu Tyr Gly 
                          100                 105                 110         
              
              
              Tyr Trp Pro Val Asp Tyr Ser Lys Pro Phe Asp Lys Lys Arg Asp Leu 
                      115                 120                 125             
              
              
              Glu Asn Ser Ile Lys Asn Met Lys Ile Ile Ser Gln Tyr Ala Glu Glu 
                  130                 135                 140                 
              
              
              Tyr Asp Ile Met Met Gly Met Glu Val Leu Asn Arg Phe Glu Gly Tyr 
              145                 150                 155                 160 
              
              
              Met Leu Asn Thr Cys Asp Glu Ala Leu Ala Tyr Val Glu Glu Val Gly 
                              165                 170                 175     
              
              
              Ser Ser Asn Val Gly Val Met Leu Asp Thr Phe His Met Asn Ile Glu 
                          180                 185                 190         
              
              
              Glu Asp Asn Ile Ala Ala Ala Ile Arg Lys Ala Gly Asp Arg Leu Tyr 
                      195                 200                 205             
              
              
              His Phe His Ile Gly Glu Gly Asn Arg Lys Val Pro Gly Lys Gly Met 
                  210                 215                 220                 
              
              
              Leu Pro Trp Asn Glu Ile Gly Gln Ala Leu Arg Asp Ile Asn Tyr Gln 
              225                 230                 235                 240 
              
              
              His Ala Ala Val Met Glu Pro Phe Val Met Gln Gly Gly Thr Val Gly 
                              245                 250                 255     
              
              
              His Asp Ile Lys Ile Trp Arg Asp Ile Ile Gly Asn Cys Ser Glu Val 
                          260                 265                 270         
              
              
              Thr Leu Asp Met Asp Ala Gln Ser Ala Leu His Phe Val Lys His Val 
                      275                 280                 285             
              
              
              Phe Glu Val 
                  290     
              
              
              <210>  9
              <211>  301
              <212>  PRT
              <213>  Clostridium bolteae 
              
              <400>  9
              
              Met Arg Tyr Phe Lys Glu Glu Val Ala Gly Met Lys Tyr Gly Ile Tyr 
              1               5                   10                  15      
              
              
              Phe Ala Tyr Trp Thr Lys Glu Trp Phe Ala Asp Tyr Lys Lys Tyr Met 
                          20                  25                  30          
              
              
              Asp Lys Val Ser Ala Leu Gly Phe Asp Val Leu Glu Ile Ser Cys Ala 
                      35                  40                  45              
              
              
              Ala Leu Arg Asp Val Tyr Thr Thr Lys Glu Gln Leu Ile Glu Leu Arg 
                  50                  55                  60                  
              
              
              Glu Tyr Ala Lys Glu Lys Gly Leu Val Leu Thr Ala Gly Tyr Gly Pro 
              65                  70                  75                  80  
              
              
              Thr Lys Ala Glu Asn Leu Cys Ser Glu Asp Pro Glu Ala Val Arg Arg 
                              85                  90                  95      
              
              
              Ala Met Thr Phe Phe Lys Asp Leu Leu Pro Lys Leu Gln Leu Met Asp 
                          100                 105                 110         
              
              
              Ile His Ile Leu Gly Gly Gly Leu Tyr Ser Tyr Trp Pro Val Asp Phe 
                      115                 120                 125             
              
              
              Thr Ile Asn Asn Asp Lys Gln Gly Asp Arg Ala Arg Ala Val Arg Asn 
                  130                 135                 140                 
              
              
              Leu Arg Glu Leu Ser Lys Thr Ala Glu Glu Cys Asp Val Val Leu Gly 
              145                 150                 155                 160 
              
              
              Met Glu Val Leu Asn Arg Tyr Glu Gly Tyr Ile Leu Asn Thr Cys Glu 
                              165                 170                 175     
              
              
              Glu Ala Ile Asp Phe Val Asp Glu Ile Gly Ser Ser His Val Lys Ile 
                          180                 185                 190         
              
              
              Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Thr Asn Met Ala Asp 
                      195                 200                 205             
              
              
              Ala Ile Arg Lys Ala Gly Asp Arg Leu Gly His Leu His Leu Gly Glu 
                  210                 215                 220                 
              
              
              Gln Asn Arg Leu Val Pro Gly Lys Gly Ser Leu Pro Trp Ala Glu Ile 
              225                 230                 235                 240 
              
              
              Gly Gln Ala Leu Arg Asp Ile Asn Tyr Gln Gly Ala Ala Val Met Glu 
                              245                 250                 255     
              
              
              Pro Phe Val Met Gln Gly Gly Thr Ile Gly Ser Glu Ile Lys Val Trp 
                          260                 265                 270         
              
              
              Arg Asp Met Val Pro Asp Leu Ser Glu Glu Ala Leu Asp Arg Asp Ala 
                      275                 280                 285             
              
              
              Lys Gly Ala Leu Glu Phe Cys Arg His Val Phe Gly Ile 
                  290                 295                 300     
              
              
              <210>  10
              <211>  290
              <212>  PRT
              <213>  Pseudomonas cichorii
              
              <400>  10
              
              Met Asn Lys Val Gly Met Phe Tyr Thr Tyr Trp Ser Thr Glu Trp Met 
              1               5                   10                  15      
              
              
              Val Asp Phe Pro Ala Thr Ala Lys Arg Ile Ala Gly Leu Gly Phe Asp 
                          20                  25                  30          
              
              
              Leu Met Glu Ile Ser Leu Gly Glu Phe His Asn Leu Ser Asp Ala Lys 
                      35                  40                  45              
              
              

              Lys Arg Glu Leu Lys Ala Val Ala Asp Asp Leu Gly Leu Thr Val Met 
                  50                  55                  60                  
              
              
              Cys Cys Ile Gly Leu Lys Ser Glu Tyr Asp Phe Ala Ser Pro Asp Lys 
              65                  70                  75                  80  
              
              
              Ser Val Arg Asp Ala Gly Thr Glu Tyr Val Lys Arg Leu Leu Asp Asp 
                              85                  90                  95      
              
              
              Cys His Leu Leu Gly Ala Pro Val Phe Ala Gly Leu Thr Phe Cys Ala 
                          100                 105                 110         
              
              
              Trp Pro Gln Ser Pro Pro Leu Asp Met Lys Asp Lys Arg Pro Tyr Val 
                      115                 120                 125             
              
              
              Asp Arg Ala Ile Glu Ser Val Arg Arg Val Ile Lys Val Ala Glu Asp 
                  130                 135                 140                 
              
              
              Tyr Gly Ile Ile Tyr Ala Leu Glu Val Val Asn Arg Phe Glu Gln Trp 
              145                 150                 155                 160 
              
              
              Leu Cys Asn Asp Ala Lys Glu Ala Ile Ala Phe Ala Asp Ala Val Asp 
                              165                 170                 175     
              
              
              Ser Pro Ala Cys Lys Val Gln Leu Asp Thr Phe His Met Asn Ile Glu 
                          180                 185                 190         
              
              
              Glu Thr Ser Phe Arg Asp Ala Ile Leu Ala Cys Lys Gly Lys Met Gly 
                      195                 200                 205             
              
              
              His Phe His Leu Gly Glu Ala Asn Arg Leu Pro Pro Gly Glu Gly Arg 
                  210                 215                 220                 
              
              
              Leu Pro Trp Asp Glu Ile Phe Gly Ala Leu Lys Glu Ile Gly Tyr Asp 
              225                 230                 235                 240 
              
              
              Gly Thr Ile Val Met Glu Pro Phe Met Arg Lys Gly Gly Ser Val Ser 
                              245                 250                 255     
              
              
              Arg Ala Val Gly Val Trp Arg Asp Met Ser Asn Gly Ala Thr Asp Glu 
                          260                 265                 270         
              
              
              Glu Met Asp Glu Arg Ala Arg Arg Ser Leu Gln Phe Val Arg Asp Lys 
                      275                 280                 285             
              
              
              Leu Ala 
                  290 
              
              
              <210>  11
              <211>  295
              <212>  PRT
              <213>  Rhodobacter sphaeroides
              
              <400>  11
              
              Met Lys Asn Pro Val Gly Ile Ile Ser Met Gln Phe Ile Arg Pro Phe 
              1               5                   10                  15      
              
              
              Thr Ser Glu Ser Leu His Phe Leu Lys Lys Ser Arg Ala Leu Gly Phe 
                          20                  25                  30          
              
              
              Asp Phe Ile Glu Leu Leu Val Pro Glu Pro Glu Asp Gly Leu Asp Ala 
                      35                  40                  45              
              
              
              Ala Glu Val Arg Arg Ile Cys Glu Gly Glu Gly Leu Gly Leu Val Leu 
                  50                  55                  60                  
              
              
              Ala Ala Arg Val Asn Leu Gln Arg Ser Ile Ala Ser Glu Glu Ala Ala 
              65                  70                  75                  80  
              
              
              Ala Arg Ala Gly Gly Arg Asp Tyr Leu Lys Tyr Cys Ile Glu Ala Ala 
                              85                  90                  95      
              
              
              Glu Ala Leu Gly Ala Thr Ile Val Gly Gly Pro Leu Tyr Gly Glu Pro 
                          100                 105                 110         
              
              
              Leu Val Phe Ala Gly Arg Pro Pro Phe Pro Trp Thr Ala Glu Gln Ile 
                      115                 120                 125             
              
              
              Ala Thr Arg Ala Ala Arg Thr Val Glu Gly Leu Ala Glu Val Ala Pro 
                  130                 135                 140                 
              
              
              Leu Ala Ala Ser Ala Gly Lys Val Phe Gly Leu Glu Pro Leu Asn Arg 
              145                 150                 155                 160 
              
              
              Phe Glu Thr Asp Ile Val Asn Thr Thr Ala Gln Ala Ile Glu Val Val 
                              165                 170                 175     
              
              
              Asp Ala Val Gly Ser Pro Gly Leu Gly Val Met Leu Asp Thr Phe His 
                          180                 185                 190         
              
              
              Met Asn Met Glu Glu Arg Ser Ile Pro Asp Ala Ile Arg Ala Thr Gly 
                      195                 200                 205             
              
              
              Ala Arg Leu Val His Phe Gln Ala Asn Glu Asn His Arg Gly Phe Pro 
                  210                 215                 220                 
              
              
              Gly Thr Gly Thr Met Asp Trp Thr Ala Ile Ala Arg Ala Leu Gly Gln 
              225                 230                 235                 240 
              
              
              Ala Gly Tyr Ala Gly Pro Val Ser Leu Glu Pro Phe Arg Arg Asp Asp 
       
                              245                 250                 255     
              
              
              Glu Arg Val Ala Leu Pro Ile Ala His Trp Arg Ala Pro His Glu Asp 
                          260                 265                 270         
              
              
              Glu Asp Glu Lys Leu Arg Ala Gly Leu Gly Leu Ile Arg Ser Ala Ile 
                      275                 280                 285             
              
              
              Thr Leu Ala Glu Val Thr His 
                  290                 295 
              
              
              <210>  12
              <211>  27
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  12
              catttacaca ctagcgaatg taatcgt                                         27
              
              
              <210>  13
              <211>  27
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  13
              catttacaca ctgccgaatg taatcgt                                         27
              
              
              <210>  14
              <211>  27
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  14
              catttacaca ctgacgaatg taatcgt                                         27
              
              
              <210>  15
              <211>  27
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  15
              catttacaca ctcgcgaatg taatcgt                                         27
              
              
              <210>  16
              <211>  27
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  16
              catttacaca cttgggaatg taatcgt                                         27
              
              
              <210>  17
              <211>  27
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  17
              catttacaca ctctcgaatg taatcgt                                         27
              
              
              <210>  18
              <211>  30
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  18
              agtgtgtaaa tgtcccaagt aagagcccgc                                      30
              
              
              <210>  19
              <211>  29
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  19
              cgccatatga aacatggtat atactacgc                                       29
              
              
              <210>  20
              <211>  25
              <212>  DNA
              <213>  artificial sequence
              
              <220>
              <223>  primer
              
              <400>  20
              cgcggatcct tgttagccgg atctc                                           25
              
              
