                         SEQUENCE LISTING

<110>  Brookhaven Science Associates LLC
       Shanklin, John
       Yu, Xiao-Hong
       Rawat Pakash, Richa
 
<120>  Engineering Cyclopropane Fatty Acid Accumulation in Plants

<130>  BSA 13-32

<150>  US 61/870,819
<151>  2013-08-28

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1164
<212>  DNA
<213>  Sterculia foetida


<220>
<221>  misc_feature
<223>  S. foetida LPAT cDNA

<400>  1
atggcgattg cagcggcagc tgtcatcgtc ccacttggcc ttctcttctt catctctggt       60

ctcgttgtca atctcattca ggcagtatgc tttgttctca ttcggccact gtccaagaaa      120

acctatagaa agatcaataa ggtggttgca gagttgttgt ggctggaact catatggctt      180

gttgattggt gggcgggagt taagattaaa gtgtttgcag atcatgaaag cttcaattta      240

atgggtaagg aacatgccct tgttgtagcc aatcacagaa gtgatattga ttggttagtt      300

ggatgggttt tggctcagcg atctggttgt cttggaagtt cagtagctgt aatgaagaaa      360

tcatcaaaat tccttccggt cataggttgg tcaatgtggt tttctgagta tctgtttttg      420

gaacgaaact gggccaagga tgaaagcacg ctaaaggcag gccttcaacg tttaaaggac      480

ttcccacagc ccttttggtt ggcacttttt gtagaaggaa ctcgctttac gcaggcaaag      540

cttctagcag ctcaagaata tgcgacctca caaggattgc ctatacctag aaatgtttta      600

attcctcgta caaagggttt tgtttcagct gtaagtcata tgcgttcatt tgtcccagcc      660

atttatgata tgacagtggc tattccaaaa agctcgcctt caccaacaat gcttagactt      720

ttcaaggggc aatcttctgt tgtgcatgta cacatcaagc ggcgtctcat gaaggaactt      780

cctgaaacgg atgaggctgt tgcacaatgg tgtaaagata tgtttgtgga gaaggacaag      840

ttgttggaca aacatattgc tgaggacact ttcagtgacc aaccattaca ctatcttggt      900

cggccaatta agcctctttt ggttgttact tcttgggcat gctttgtggc ttatggagct      960

ctcaaatttc tgcaatggtc ttcactttta tcctcatgga aagggattgc attttcagct     1020

tttggcttgg ccatcgttac catccttatg catatcttga tactcttctc tcagtcagag     1080

cgttcaactc ctgccaaggt tgcaccgggg aagcccaaga atgaccagga gaatttggag     1140

gcaagacgag acaaacagca gtag                                            1164


<210>  2
<211>  387
<212>  PRT
<213>  Sterculia foetida


<220>
<221>  misc_feature
<223>  S. foetida LPAT

<400>  2

Met Ala Ile Ala Ala Ala Ala Val Ile Val Pro Leu Gly Leu Leu Phe 
1               5                   10                  15      


Phe Ile Ser Gly Leu Val Val Asn Leu Ile Gln Ala Val Cys Phe Val 
            20                  25                  30          


Leu Ile Arg Pro Leu Ser Lys Lys Thr Tyr Arg Lys Ile Asn Lys Val 
        35                  40                  45              


Val Ala Glu Leu Leu Trp Leu Glu Leu Ile Trp Leu Val Asp Trp Trp 
    50                  55                  60                  


Ala Gly Val Lys Ile Lys Val Phe Ala Asp His Glu Ser Phe Asn Leu 
65                  70                  75                  80  


Met Gly Lys Glu His Ala Leu Val Val Ala Asn His Arg Ser Asp Ile 
                85                  90                  95      


Asp Trp Leu Val Gly Trp Val Leu Ala Gln Arg Ser Gly Cys Leu Gly 
            100                 105                 110         


Ser Ser Val Ala Val Met Lys Lys Ser Ser Lys Phe Leu Pro Val Ile 
        115                 120                 125             


Gly Trp Ser Met Trp Phe Ser Glu Tyr Leu Phe Leu Glu Arg Asn Trp 
    130                 135                 140                 


Ala Lys Asp Glu Ser Thr Leu Lys Ala Gly Leu Gln Arg Leu Lys Asp 
145                 150                 155                 160 


Phe Pro Gln Pro Phe Trp Leu Ala Leu Phe Val Glu Gly Thr Arg Phe 
                165                 170                 175     


Thr Gln Ala Lys Leu Leu Ala Ala Gln Glu Tyr Ala Thr Ser Gln Gly 
            180                 185                 190         


Leu Pro Ile Pro Arg Asn Val Leu Ile Pro Arg Thr Lys Gly Phe Val 
        195                 200                 205             


Ser Ala Val Ser His Met Arg Ser Phe Val Pro Ala Ile Tyr Asp Met 
    210                 215                 220                 


Thr Val Ala Ile Pro Lys Ser Ser Pro Ser Pro Thr Met Leu Arg Leu 
225                 230                 235                 240 


Phe Lys Gly Gln Ser Ser Val Val His Val His Ile Lys Arg Arg Leu 
                245                 250                 255     


Met Lys Glu Leu Pro Glu Thr Asp Glu Ala Val Ala Gln Trp Cys Lys 
            260                 265                 270         


Asp Met Phe Val Glu Lys Asp Lys Leu Leu Asp Lys His Ile Ala Glu 
        275                 280                 285             


Asp Thr Phe Ser Asp Gln Pro Leu His Tyr Leu Gly Arg Pro Ile Lys 
    290                 295                 300                 


Pro Leu Leu Val Val Thr Ser Trp Ala Cys Phe Val Ala Tyr Gly Ala 
305                 310                 315                 320 


Leu Lys Phe Leu Gln Trp Ser Ser Leu Leu Ser Ser Trp Lys Gly Ile 
                325                 330                 335     


Ala Phe Ser Ala Phe Gly Leu Ala Ile Val Thr Ile Leu Met His Ile 
            340                 345                 350         


Leu Ile Leu Phe Ser Gln Ser Glu Arg Ser Thr Pro Ala Lys Val Ala 
        355                 360                 365             


Pro Gly Lys Pro Lys Asn Asp Gln Glu Asn Leu Glu Ala Arg Arg Asp 
    370                 375                 380                 


Lys Gln Gln 
385         


<210>  3
<211>  999
<212>  DNA
<213>  Sterculia foetida


<220>
<221>  misc_feature
<223>  S. foetida DGAT cDNA

<400>  3
atgggggaag aaagggagga gagaaaggcg gctggacctg gacctgaacc tgggtatagg       60

gtattcagtg gaagagatga attcccgtcc aacatggtgc acagcgtaat agctattgcg      120

ttatggcttt ggactattca tttcaacgcc ctcttgcttc ttctctccct tatattcctg      180

cctttttcca aattccttgt ggtgttcgga tttcttttgg ttttcgtgtt tcttcctatt      240

gatcctgaca gtaaatttgg tcggcgcttg ggcaggtata tatgcaagca tctttgcagt      300

tattttccca ccactctcca cgttgaggac attcacgcct tccatcctga tcgtgcttac      360

gtttttggtt atgagccaca ttcaatttgg ccaatcggag ttgttacact tgctgaactt      420

acaggtttcc tacctcttcc gaaaatgaaa gtccttgcta ccagcgttgt attctacact      480

ccatttttgc ggcatatatg gacatggttg ggtgtctcac cagctacaag gaaaactttt      540

tattccctat tggatgctgg ttatagttgt attatagtgc ctggtggagt gcaggagata      600

taccacatgg agcttggttc tgaggttgcg ttccttaggg cacgaaaagg atttgttcgc      660

atagccatgg aaaagggctg tccactggtt ccagttttct gttttggtca gtcccatgcc      720

tacaagtggt ggaaaccaag cgggaagttg tacctgcaat tttcccgagc tatcaagttc      780

attccgatat tcttttgggg aattcttgga actcctttac cctatcaaca tccaatgcat      840

gtggtggtgg gtaaacctat tgacttgaag agaaatccac aacctactga cgaagaggtt      900

cttgaagtac atcggcaatt tgttcaagca cttcaagatc tctttgagag acacaaggct      960

cgggttggat atgctgatct tccattaaag attctttga                             999


<210>  4
<211>  332
<212>  PRT
<213>  Sterculia foetida


<220>
<221>  misc_feature
<223>  S. foetida DGAT

<400>  4

Met Gly Glu Glu Arg Glu Glu Arg Lys Ala Ala Gly Pro Gly Pro Glu 
1               5                   10                  15      


Pro Gly Tyr Arg Val Phe Ser Gly Arg Asp Glu Phe Pro Ser Asn Met 
            20                  25                  30          


Val His Ser Val Ile Ala Ile Ala Leu Trp Leu Trp Thr Ile His Phe 
        35                  40                  45              


Asn Ala Leu Leu Leu Leu Leu Ser Leu Ile Phe Leu Pro Phe Ser Lys 
    50                  55                  60                  


Phe Leu Val Val Phe Gly Phe Leu Leu Val Phe Val Phe Leu Pro Ile 
65                  70                  75                  80  


Asp Pro Asp Ser Lys Phe Gly Arg Arg Leu Gly Arg Tyr Ile Cys Lys 
                85                  90                  95      


His Leu Cys Ser Tyr Phe Pro Thr Thr Leu His Val Glu Asp Ile His 
            100                 105                 110         


Ala Phe His Pro Asp Arg Ala Tyr Val Phe Gly Tyr Glu Pro His Ser 
        115                 120                 125             


Ile Trp Pro Ile Gly Val Val Thr Leu Ala Glu Leu Thr Gly Phe Leu 
    130                 135                 140                 


Pro Leu Pro Lys Met Lys Val Leu Ala Thr Ser Val Val Phe Tyr Thr 
145                 150                 155                 160 


Pro Phe Leu Arg His Ile Trp Thr Trp Leu Gly Val Ser Pro Ala Thr 
                165                 170                 175     


Arg Lys Thr Phe Tyr Ser Leu Leu Asp Ala Gly Tyr Ser Cys Ile Ile 
            180                 185                 190         


Val Pro Gly Gly Val Gln Glu Ile Tyr His Met Glu Leu Gly Ser Glu 
        195                 200                 205             


Val Ala Phe Leu Arg Ala Arg Lys Gly Phe Val Arg Ile Ala Met Glu 
    210                 215                 220                 


Lys Gly Cys Pro Leu Val Pro Val Phe Cys Phe Gly Gln Ser His Ala 
225                 230                 235                 240 


Tyr Lys Trp Trp Lys Pro Ser Gly Lys Leu Tyr Leu Gln Phe Ser Arg 
                245                 250                 255     


Ala Ile Lys Phe Ile Pro Ile Phe Phe Trp Gly Ile Leu Gly Thr Pro 
            260                 265                 270         


Leu Pro Tyr Gln His Pro Met His Val Val Val Gly Lys Pro Ile Asp 
        275                 280                 285             


Leu Lys Arg Asn Pro Gln Pro Thr Asp Glu Glu Val Leu Glu Val His 
    290                 295                 300                 


Arg Gln Phe Val Gln Ala Leu Gln Asp Leu Phe Glu Arg His Lys Ala 
305                 310                 315                 320 


Arg Val Gly Tyr Ala Asp Leu Pro Leu Lys Ile Leu 
                325                 330         


<210>  5
<211>  2169
<212>  DNA
<213>  Sterculia foetida


<220>
<221>  misc_feature
<223>  S. foetida PDAT cDNA

<400>  5
atgaccatta tggtcgccgg cggcaaggta cagaaagaag gcactttatc cggtcaatgt       60

gaacaatccc agaatcagaa ttctcagtct ctattaatga gcaaaccagt tcaaccgcag      120

agtctttata gatctcattt tgggttccaa acaatgtcgt ttttgcggcg gaggaaggta      180

acggagtcct cgaaatcctc aagctccgaa cccaacatcg aaaacgaagc ggacaagaaa      240

aaccgataca agagcccgca aagaaaacgt tatagttcga agaaatggtc gtgttgggac      300

agatgttgtt ggtacattgg ttttatgtgt tcgctgtggt ggttcttgtt gttcttatac      360

aatgcaatgc cggcttcatt cccgcaatac gtaacgcagg cgataactgg gccgttgccg      420

gacccgcctg gggttaagtt gaggaaagag ggcttgacgg tgaatcaccc ggtggtatta      480

gtgcctgggg ttgtgactgg tggacttgag ctttgggaag ggcgggagtg tgcgaaaggg      540

ttgcttggga aacgcctttg gggtggctac tttggagaac tgtataaaag acccttatgc      600

tggcttgagc acatctcact tgataatgaa actggactgg accctcctgg tataagggtc      660

aggcctgtat ctggacttgt ggcagcagat tatttcgcag caggttattt tgtttgggct      720

gttttaattg ctaatttggc tcatattggg tatgaggaaa aaaacatgta tatggctgct      780

tatgattgga ggttatcttt tcagaacacg gaggtcaggg accaaacttt aaccagaata      840

aaaagtaaca tagaactctt ggtagctaca aatggtggga aaaaggtagt agtccttcca      900

cattcaatgg gagtccagta ctttctgcac ttcatgaaat gggttgaagc accacctccc      960

gtgggtggtg gaggtggatc agattggtgt gctaggcaca taaaggcagt agtgaacatt     1020

ggtgcaccct ttttaggttg tccgaaatcg gtcccactgc atttttctat cgaagtcaag     1080

gatatagcga atctcagggc ttttgcacca ggttttctgg aaaaagatgt acttggtctc     1140

aaaatgtttc agcatttaat gcggatgttc cgtacgtggg atgccaccag gtcaatgata     1200

ccaaaaggtg gggaaactat ctggggtggg cttgattggt cacctgaagt aggaagcttt     1260

aactctagtg cgaaaaaatt gaagaacaat agcacccata atacaggcca aaactcaaac     1320

agctattttt gtgatatgaa aggcgtgaat tatgggagaa ttatttcatt tgggaaagat     1380

gtggctgagg cagattcctc cacaatagag agggttgatt tcagggatgc tgtaaagagc     1440

gataagcttg ccaactcaag caactgtgat gtatggatag agtatcatga attgggcaat     1500

ggagatatca aagcagttgc tgattcaaaa gtttacactg ctggatcatt tttggatctg     1560

cttcgttttg ttgcccccaa gtggatggaa cggggtgatg ctcatttttc gtatgggata     1620

gcagatgatt tggatgaccc aaagtatgaa cactacaaat attggtcaaa ccccttagaa     1680

acaaagttac caaatgctcc aaacatggaa atctactcaa tgtatggagt tggactcccc     1740

actgaaagag cttatatcta caaattaact actgccactg attgctatat tccgtttgag     1800

atagacatct cagcagaggg tggtagtgaa gattcatgtc taaaaggtgg tgttttctct     1860

gttgatggag atgaatccgt tcctgtttta agtgcaggtt tcacgtgtgc aaaagcttgg     1920

cggggtaaaa ccagattcaa tccttcaggg attcgtactt acataaggga gtacaatcat     1980

gcccctccag ctagtcttct agaaggtcga ggcacgcaaa gtggttctca tgttgatata     2040

ttggggaatt ttgctttgat tgaggatgtt atccgaatag cagcaggggc tactggtgag     2100

gacctgggtg gagatcgtgt ttattctgat atttttgaat ggtctgaaag gatcaactta     2160

aagctttag                                                             2169


<210>  6
<211>  722
<212>  PRT
<213>  Sterculia foetida


<220>
<221>  misc_feature
<223>  S. foetida PDAT

<400>  6

Met Thr Ile Met Val Ala Gly Gly Lys Val Gln Lys Glu Gly Thr Leu 
1               5                   10                  15      


Ser Gly Gln Cys Glu Gln Ser Gln Asn Gln Asn Ser Gln Ser Leu Leu 
            20                  25                  30          


Met Ser Lys Pro Val Gln Pro Gln Ser Leu Tyr Arg Ser His Phe Gly 
        35                  40                  45              


Phe Gln Thr Met Ser Phe Leu Arg Arg Arg Lys Val Thr Glu Ser Ser 
    50                  55                  60                  


Lys Ser Ser Ser Ser Glu Pro Asn Ile Glu Asn Glu Ala Asp Lys Lys 
65                  70                  75                  80  


Asn Arg Tyr Lys Ser Pro Gln Arg Lys Arg Tyr Ser Ser Lys Lys Trp 
                85                  90                  95      


Ser Cys Trp Asp Arg Cys Cys Trp Tyr Ile Gly Phe Met Cys Ser Leu 
            100                 105                 110         


Trp Trp Phe Leu Leu Phe Leu Tyr Asn Ala Met Pro Ala Ser Phe Pro 
        115                 120                 125             


Gln Tyr Val Thr Gln Ala Ile Thr Gly Pro Leu Pro Asp Pro Pro Gly 
    130                 135                 140                 


Val Lys Leu Arg Lys Glu Gly Leu Thr Val Asn His Pro Val Val Leu 
145                 150                 155                 160 


Val Pro Gly Val Val Thr Gly Gly Leu Glu Leu Trp Glu Gly Arg Glu 
                165                 170                 175     


Cys Ala Lys Gly Leu Leu Gly Lys Arg Leu Trp Gly Gly Tyr Phe Gly 
            180                 185                 190         


Glu Leu Tyr Lys Arg Pro Leu Cys Trp Leu Glu His Ile Ser Leu Asp 
        195                 200                 205             


Asn Glu Thr Gly Leu Asp Pro Pro Gly Ile Arg Val Arg Pro Val Ser 
    210                 215                 220                 


Gly Leu Val Ala Ala Asp Tyr Phe Ala Ala Gly Tyr Phe Val Trp Ala 
225                 230                 235                 240 


Val Leu Ile Ala Asn Leu Ala His Ile Gly Tyr Glu Glu Lys Asn Met 
                245                 250                 255     


Tyr Met Ala Ala Tyr Asp Trp Arg Leu Ser Phe Gln Asn Thr Glu Val 
            260                 265                 270         


Arg Asp Gln Thr Leu Thr Arg Ile Lys Ser Asn Ile Glu Leu Leu Val 
        275                 280                 285             


Ala Thr Asn Gly Gly Lys Lys Val Val Val Leu Pro His Ser Met Gly 
    290                 295                 300                 


Val Gln Tyr Phe Leu His Phe Met Lys Trp Val Glu Ala Pro Pro Pro 
305                 310                 315                 320 


Val Gly Gly Gly Gly Gly Ser Asp Trp Cys Ala Arg His Ile Lys Ala 
                325                 330                 335     


Val Val Asn Ile Gly Ala Pro Phe Leu Gly Cys Pro Lys Ser Val Pro 
            340                 345                 350         


Leu His Phe Ser Ile Glu Val Lys Asp Ile Ala Asn Leu Arg Ala Phe 
        355                 360                 365             


Ala Pro Gly Phe Leu Glu Lys Asp Val Leu Gly Leu Lys Met Phe Gln 
    370                 375                 380                 


His Leu Met Arg Met Phe Arg Thr Trp Asp Ala Thr Arg Ser Met Ile 
385                 390                 395                 400 


Pro Lys Gly Gly Glu Thr Ile Trp Gly Gly Leu Asp Trp Ser Pro Glu 
                405                 410                 415     


Val Gly Ser Phe Asn Ser Ser Ala Lys Lys Leu Lys Asn Asn Ser Thr 
            420                 425                 430         


His Asn Thr Gly Gln Asn Ser Asn Ser Tyr Phe Cys Asp Met Lys Gly 
        435                 440                 445             


Val Asn Tyr Gly Arg Ile Ile Ser Phe Gly Lys Asp Val Ala Glu Ala 
    450                 455                 460                 


Asp Ser Ser Thr Ile Glu Arg Val Asp Phe Arg Asp Ala Val Lys Ser 
465                 470                 475                 480 


Asp Lys Leu Ala Asn Ser Ser Asn Cys Asp Val Trp Ile Glu Tyr His 
                485                 490                 495     


Glu Leu Gly Asn Gly Asp Ile Lys Ala Val Ala Asp Ser Lys Val Tyr 
            500                 505                 510         


Thr Ala Gly Ser Phe Leu Asp Leu Leu Arg Phe Val Ala Pro Lys Trp 
        515                 520                 525             


Met Glu Arg Gly Asp Ala His Phe Ser Tyr Gly Ile Ala Asp Asp Leu 
    530                 535                 540                 


Asp Asp Pro Lys Tyr Glu His Tyr Lys Tyr Trp Ser Asn Pro Leu Glu 
545                 550                 555                 560 


Thr Lys Leu Pro Asn Ala Pro Asn Met Glu Ile Tyr Ser Met Tyr Gly 
                565                 570                 575     


Val Gly Leu Pro Thr Glu Arg Ala Tyr Ile Tyr Lys Leu Thr Thr Ala 
            580                 585                 590         


Thr Asp Cys Tyr Ile Pro Phe Glu Ile Asp Ile Ser Ala Glu Gly Gly 
        595                 600                 605             


Ser Glu Asp Ser Cys Leu Lys Gly Gly Val Phe Ser Val Asp Gly Asp 
    610                 615                 620                 


Glu Ser Val Pro Val Leu Ser Ala Gly Phe Thr Cys Ala Lys Ala Trp 
625                 630                 635                 640 


Arg Gly Lys Thr Arg Phe Asn Pro Ser Gly Ile Arg Thr Tyr Ile Arg 
                645                 650                 655     


Glu Tyr Asn His Ala Pro Pro Ala Ser Leu Leu Glu Gly Arg Gly Thr 
            660                 665                 670         


Gln Ser Gly Ser His Val Asp Ile Leu Gly Asn Phe Ala Leu Ile Glu 
        675                 680                 685             


Asp Val Ile Arg Ile Ala Ala Gly Ala Thr Gly Glu Asp Leu Gly Gly 
    690                 695                 700                 


Asp Arg Val Tyr Ser Asp Ile Phe Glu Trp Ser Glu Arg Ile Asn Leu 
705                 710                 715                 720 


Lys Leu 
        


