               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> GLYCEROL AND ACETIC ACID CONVERTING CELLS WITH IMPROVED GLYCEROL TRANSPORT

<130> 29961-EP-EPA

<150> EPEP13182225.6
<151> 2013-08-29

<160> 43

<170> BiSSAP 1.2

<210> 1
<211> 5125
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5125
<223> /organism="Artificial Sequence"
      /note="Sequence containing the acdH-gene of L. plantarum under
      control of the TDH3-promoter and the PGK1-terminator"
      /mol_type="unassigned DNA"

<400> 1
gtgcttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg       60

gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc      120

atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc      180

agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca      240

actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc      300

aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt      360

ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt      420

tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg      480

tattgattgt aattctgtaa atctatttct taaacttctt aaattctact tttatagtta      540

gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa      600

caaaatgttg aaggaaatgg aagaaactac tgtttctcgt tccattgaca gattggtttt      660

gaatgcctcc ttggctgcca acagattgga agtcatggac caatctcaag ttgaccaagc      720

tgttgccgct atggccagag ccgctcacgc tgctcgtggt atgttggctg ctatggctgt      780

cgaagaaacc ggtagaggta actacagaga caaggttgcc aaaaacgatt ttgctgccaa      840

gaacgtctac aactacatca aggatgacaa gactgtcggt atcatcaacg atgaccctgt      900

ttctggtgtc atgaaggtcg ctgaacctgt tggtatcatt gccggtgtta ccccagtcac      960

caacccaact tccactgtca tcttcaacgc tatgttagct ttgaaaacca gaaacccaat     1020

tatctttggt ttccacccat tcgctcaaaa gtcttgtgtt gaaactggta gaatcatcag     1080

agatgctgct attgcttctg gtgctccaaa ggactggatc caatggatca agaccccatc     1140

tttggaagct accaacactt tgatgaacca tccaggtgtt gccactatca ttgctaccgg     1200

tggtgccggt atggtcaaga ccgcttactc cactggtaag ccagctttag gtgtcggtcc     1260

aggtaacgtc ccatgtttca ttgaacaaac cgctgatatc caacaagctg tttccgatgt     1320

cgttacctcc aagtctttcg ataacggtat gatctgtgct tctgaatcca acttgattgt     1380

tgctgaccaa atctacgacc aagtcaagag agaattgtct cacaacggtg tctacttcgt     1440

tggtactgaa aacttcaagg ctctagaagc tactgttatg aacttggaca agcaagctgt     1500

cgatccaaag gttgccggtc aaaccccatg gcaaattgcc caatgggctg gtttcgatgt     1560

cccatctgac accaaggttt tagctgttga attgccatcc attggtggtg accaagtctt     1620

gtccagagaa aagttgtctc cagttttggc tgtcgttcac gctaaggaca ctgaagctgg     1680

tttcaacttg atgaagagat ctttggcttt gggtggttta ggtcacaccg ccgctttgca     1740

caccactgac gaagctgtca tgaacaaatt cgctttggaa atgaccgctt gtagagcttt     1800

gatcaacgtt ccatcttctc aaggtgccat tggttacaaa tacgacaatg ttgctccatc     1860

tttgactttg ggttgtggta cttggggtca caactccatc tctcacaact tggaagattg     1920

ggacttgttg aacatcaaga ctgttgctaa gagattaacc aaaataagat aaattgaatt     1980

gaattgaaat cgatagatca atttttttct tttctctttc cccatccttt acgctaaaat     2040

aatagtttat tttatttttt gaatattttt tatttatata cgtatatata gactattatt     2100

tatcttttaa tgattattaa gatttttatt aaaaaaaatt acgctcctct tttaatgcct     2160

ttatgcagtt ttttttccca ttcgatattt ctatgttcgg gttcagcgta ttttaagttt     2220

aataactcga aaattctgcg ttcgttaaag ctttcgagaa ggatattatt tccctcttgc     2280

ccatcgaacg tacaagtact cctctgttct ctccttcctt tgctttaaaa tgaagtgaag     2340

ttcctatact ttctagagaa taggaacttc tatagtgagt cgaataaggg cgacacaaaa     2400

tttattctaa atgcataata aatactgata acatcttata gtttgtatta tattttgtat     2460

tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt attttcgaga     2520

tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa aatcataaat     2580

aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt atcttattta     2640

aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc aagcaaagtg     2700

acaggcgccc ttaaatattc tgacaaatgc tctttcccta aactcccccc ataaaaaaac     2760

ccgccgaagc gggtttttac gttatttgcg gattaacgat tactcgttat cagaaccgcc     2820

cagggggccc gagcttaaga ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac     2880

gcaaaaaggc catccgtcag gggccttctg cttagtttga tgcctggcag ttccctactc     2940

tcgccttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     3000

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     3060

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     3120

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     3180

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     3240

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     3300

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     3360

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     3420

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     3480

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     3540

tgggctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     3600

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     3660

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     3720

cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt     3780

aagggatttt ggtcatgagc ttgcgccgtc ccgtcaagtc agcgtaatgc tctgctttta     3840

gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc     3900

atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag     3960

gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat     4020

taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga     4080

atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc     4140

attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc     4200

ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgagtg     4260

caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc     4320

ttctaatacc tggaacgctg tttttccggg gatcgcagtg gtgagtaacc atgcatcatc     4380

aggagtacgg ataaaatgct tgatggtcgg aagtggcata aattccgtca gccagtttag     4440

tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa     4500

ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt gcccgacatt     4560

atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct     4620

cgacgtttcc cgttgaatat ggctcatatt cttccttttt caatattatt gaagcattta     4680

tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     4740

aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc catttatacc     4800

tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg gtcccacctg     4860

accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggactcccc     4920

atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg     4980

gcctttcgcc cgggctaatt agggggtgtc gcccttattc gactctatag tgaagttcct     5040

attctctaga aagtatagga acttctgaag tggggaagcg acttccaatc gctttgcata     5100

tccagtacca cacccacagg cgttt                                           5125


<210> 2
<211> 5810
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5810
<223> /organism="Artificial Sequence"
      /note="Sequence containing the ACS2-gene of S. cerevisiae under
      control of the PGK1-promoter and the PGI1-terminator"
      /mol_type="unassigned DNA"

<400> 2
gtgcgggcca gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac cctcataaag       60

cacgtggcct cttatcgaga aagaaattac cgtcgctcgt gatttgtttg caaaaagaac      120

aaaactgaaa aaacccagac acgctcgact tcctgtcttc ctattgattg cagcttccaa      180

tttcgtcaca caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag caatcgaagg      240

ttctggaatg gcgggaaagg gtttagtacc acatgctatg atgcccactg tgatctccag      300

agcaaagttc gttcgatcgt actgttactc tctctctttc aaacagaatt gtccgaatcg      360

tgtgacaaca acagcctgtt ctcacacact cttttcttct aaccaagggg gtggtttagt      420

ttagtagaac ctcgtgaaac ttacatttac atatatataa acttgcataa attggtcaat      480

gcaagaaata catatttggt cttttctaat tcgtagtttt tcaagttctt agatgctttc      540

tttttctctt ttttacagat catcaaggaa gtaattatct actttttaca acaaatataa      600

aacaatgacc atcaaggaac acaaggttgt ctacgaagct cacaacgtca aggctttgaa      660

agctcctcaa catttctaca actctcaacc aggtaagggt tacgttaccg atatgcaaca      720

ctaccaagaa atgtaccaac aatccatcaa cgaaccagaa aaattcttcg acaagatggc      780

taaggaatac ttgcactggg atgctccata caccaaggtt caatccggtt ccttgaacaa      840

cggtgatgtc gcttggttct tgaacggtaa attgaatgct tcctacaact gtgtcgatcg      900

tcacgctttc gctaacccag acaaaccagc tttgatttac gaagctgatg acgaatctga      960

caacaagatc atcactttcg gtgaactatt aagaaaggtt tctcaaatcg ctggtgtctt     1020

gaaatcctgg ggtgttaaga agggtgacac tgttgccatc tacttgccaa tgatcccaga     1080

agctgttatt gctatgttgg ctgtcgccag aattggtgcc attcactctg ttgttttcgc     1140

tggtttctct gccggttctt tgaaggacag agtcgttgac gctaactcta aggttgtcat     1200

cacttgtgac gaaggtaaga gaggtggtaa gaccattaac actaagaaga ttgtcgacga     1260

aggtttgaac ggtgttgatt tggtttccag aatcttggtt ttccaaagaa ccggtactga     1320

aggtattcca atgaaggctg gtagagacta ctggtggcat gaagaagccg ctaagcaaag     1380

aacttaccta ccacctgttt cctgtgatgc tgaagatcca ttgtttttgt tgtacacctc     1440

tggttctacc ggttctccaa agggtgttgt ccacactacc ggtggttact tgttgggtgc     1500

tgctttgacc accagatacg tctttgacat ccaccctgaa gatgtcttat tcactgctgg     1560

tgacgtcggt tggatcactg gtcataccta cgctttgtac ggtccattga ctttaggtac     1620

tgcttctatc atcttcgaat ccaccccagc ttacccagac tacggtagat actggagaat     1680

cattcaaaga cacaaggcca cccacttcta cgtcgcccca actgccttga gattaatcaa     1740

gagagttggt gaagctgaaa ttgctaaata cgatacctct tctttgagag tcttaggttc     1800

tgtcggtgaa ccaatttctc cagacttatg ggaatggtat cacgaaaagg ttggtaacaa     1860

gaactgtgtc atctgtgaca ccatgtggca aactgaatct ggttctcact tgattgctcc     1920

attggctggt gctgttccaa ctaagccagg ttccgctacc gttccattct tcggtatcaa     1980

cgcttgtatc attgacccag tcactggtgt cgaattggaa ggtaacgatg ttgaaggtgt     2040

cttggccgtc aagtctccat ggccatccat ggctagatct gtctggaacc accacgatcg     2100

ttacatggac acctacttga aaccataccc aggtcactac ttcaccggtg atggtgccgg     2160

tcgtgaccac gatggttact actggatcag aggtcgtgtt gatgacgttg ttaacgtttc     2220

tggtcacaga ttgtccactt ctgaaatcga agcctccatc tccaaccatg aaaacgtttc     2280

cgaagctgct gtcgttggta ttccagacga attgaccggt caaactgttg ttgcttacgt     2340

ttctttaaag gacggttact tgcaaaacaa cgccactgaa ggtgacgctg aacacatcac     2400

tccagataac ttaagaagag aattgatttt gcaagttcgt ggtgaaattg gtccatttgc     2460

ttccccaaag accatcattt tggttagaga cttgccaaga accagatctg gtaagatcat     2520

gagaagagtc ttgagaaagg ttgcctccaa tgaggctgaa caattgggtg acttgactac     2580

tttggccaac ccagaagtcg tcccagctat catttctgct gtcgaaaacc aattcttctc     2640

ccaaaagaag aaataaaaca aatcgctctt aaatatatac ctaaagaaca ttaaagctat     2700

attataagca aagatacgta aattttgctt atattattat acacatatca tatttctata     2760

tttttaagat ttggttatat aatgtacgta atgcaaagga aataaatttt atacattatt     2820

gaacagcgtc caagtaacta cattatgtgc actaatagtt tagcgtcgtg aagactttat     2880

tgtgtcgcga aaagtaaaaa ttttaaaaat tagagcacct tgaacttgcg aaaaaggttc     2940

tcatcaactg tttaaaacct cacgctttcc ggcatcttcc agaccacagt atatccatcc     3000

gcctcctgtt gaaaatgaag tgaagttcct atactttcta gagaatagga acttctatag     3060

tgagtcgaat aagggcgaca caaaatttat tctaaatgca taataaatac tgataacatc     3120

ttatagtttg tattatattt tgtattatcg ttgacatgta taattttgat atcaaaaact     3180

gattttccct ttattatttt cgagatttat tttcttaatt ctctttaaca aactagaaat     3240

attgtatata caaaaaatca taaataatag atgaatagtt taattatagg tgttcatcaa     3300

tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt ttctcattta taaggttaaa     3360

taattctcat atatcaagca aagtgacagg cgcccttaaa tattctgaca aatgctcttt     3420

ccctaaactc cccccataaa aaaacccgcc gaagcgggtt tttacgttat ttgcggatta     3480

acgattactc gttatcagaa ccgcccaggg ggcccgagct taagactggc cgtcgtttta     3540

caacacagaa agagtttgta gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag     3600

tttgatgcct ggcagttccc tactctcgcc ttccgcttcc tcgctcactg actcgctgcg     3660

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     3720

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     3780

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     3840

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     3900

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     3960

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     4020

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     4080

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     4140

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     4200

cggtgctaca gagttcttga agtggtgggc taactacggc tacactagaa gaacagtatt     4260

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     4320

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     4380

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     4440

gaacgacgcg cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc     4500

aagtcagcgt aatgctctgc ttttagaaaa actcatcgag catcaaatga aactgcaatt     4560

tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag     4620

aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga     4680

ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg     4740

agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt     4800

tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca     4860

aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag     4920

gacaattaca aacaggaatc gagtgcaacc ggcgcaggaa cactgccagc gcatcaacaa     4980

tattttcacc tgaatcagga tattcttcta atacctggaa cgctgttttt ccggggatcg     5040

cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagtg     5100

gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc     5160

tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga     5220

ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat     5280

ccatgttgga atttaatcgc ggcctcgacg tttcccgttg aatatggctc atattcttcc     5340

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg     5400

aatgtattta gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga     5460

acattatcgc gagcccattt atacctgaat atggctcata acaccccttg tttgcctggc     5520

ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc     5580

gccgatggta gtgtggggac tccccatgcg agagtaggga actgccaggc atcaaataaa     5640

acgaaaggct cagtcgaaag actgggcctt tcgcccgggc taattagggg gtgtcgccct     5700

tattcgactc tatagtgaag ttcctattct ctagaaagta taggaacttc tgaagtgggg     5760

cggatcgatg tacacaaccg actgcaccca aacgaacaca aatcttagca                5810


<210> 3
<211> 4862
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4862
<223> /organism="Artificial Sequence"
      /note="Sequence containing the gldA-gene of E. coli under control
      of the ENO1-promoter and the CYC1-terminator"
      /mol_type="unassigned DNA"

<400> 3
gtgcccgcgg aaccgccaga tattcattac ttgacgcaaa agcgtttgaa ataatgacga       60

aaaagaagga agaaaaaaaa agaaaaatac cgcttctagg cgggttatct actgatccga      120

gcttccacta ggatagcacc caaacacctg catatttgga cgacctttac ttacaccacc      180

aaaaaccact ttcgcctctc ccgcccctga taacgtccac taattgagcg attacctgag      240

cggtcctctt ttgtttgcag catgagactt gcatactgca aatcgtaagt agcaacgtgt      300

caaggtcaaa actgtatgga aaccttgtca cctcacttaa ttctagctag cctaccctgc      360

aagtcaagag gtgtccgtga ttcctagcca cctcaaggta tgcctctccc cggaaactgt      420

ggccttttct ggcacacatg atctccacga tttcaacata taaatagctt ttgataatgg      480

caatattaat caaatttatt ttacttcttt cttgtaacat ctctcttgta atcccttatt      540

ccttctagct atttttcata aaaaaccaag caactgctta tcaacacaca aacactaaat      600

caaaatggac agaatcatcc aatctccagg taagtacatc caaggtgctg atgttatcaa      660

cagattaggt gaatacttga agccattggc tgaaagatgg ttagtcgtcg gtgacaaatt      720

cgttttgggt ttcgctcaat ccaccgtcga aaagtctttc aaggatgctg gtttggttgt      780

tgaaatcgct ccattcggtg gtgaatgttc tcaaaatgaa attgaccgtt tgagaggtat      840

tgctgaaact gctcaatgtg gtgccatctt gggtattggt ggtggtaaga ctttggacac      900

tgccaaggct ttggcccact tcatgggtgt tccagttgcc attgctccaa ccattgcttc      960

taccgatgct ccatgttctg ctttgtccgt tatctacacc gacgaaggtg aatttgaccg     1020

ttacttgttg ttgccaaaca acccaaacat ggtcattgtc gacaccaaga tcgttgccgg     1080

tgctccagcc agattattgg ctgccggtat cggtgatgct ttggctacct ggttcgaagc     1140

cagagcttgt tccagatctg gtgctactac catggccggt ggtaaatgta ctcaagctgc     1200

tttagctttg gctgaattgt gttacaacac tttgttggaa gaaggcgaaa aggctatgtt     1260

ggctgctgaa caacacgttg ttactccagc tttggaaaga gtcattgaag ccaacaccta     1320

cttgtccggt gttggattcg aatctggtgg tttagctgcc gctcatgccg tccacaacgg     1380

tttgactgcc atcccagatg ctcaccacta ctaccacggt gaaaaggttg ctttcggtac     1440

tttgactcaa ttagtcttgg aaaacgctcc agtcgaagaa atcgaaaccg ttgctgctct     1500

atcccacgct gtcggtttgc ctatcacttt ggctcaattg gacatcaagg aagatgtccc     1560

agctaagatg agaattgttg ctgaagctgc ttgtgctgaa ggtgaaacca ttcacaacat     1620

gccaggtggt gccaccccag accaagtcta cgctgctttg ttggttgctg accaatacgg     1680

tcaaagattc ttgcaagaat gggagtaaaa caggcccctt ttcctttgtc gatatcatgt     1740

aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg     1800

aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat     1860

taagaacgtt atttatattt caaatttttc ttttttttct gtacaaacgc gtgtacgcat     1920

gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt     1980

gcaagcttcg cagtttacac tctcatcgtc ctcaacgttg tccaggtttg tatccacgtg     2040

tgtccgttcc gccaatattc cgcaaaatga agtgaagttc ctatactttc tagagaatag     2100

gaacttctat agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat     2160

actgataaca tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg     2220

atatcaaaaa ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa     2280

caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata     2340

ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt     2400

tataaggtta aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga     2460

caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt     2520

atttgcggat taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg     2580

gccgtcgttt tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg     2640

ccttctgctt agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac     2700

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     2760

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     2820

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     2880

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     2940

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     3000

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     3060

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     3120

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     3180

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     3240

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag     3300

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     3360

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     3420

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     3480

tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagcttg     3540

cgccgtcccg tcaagtcagc gtaatgctct gcttttagaa aaactcatcg agcatcaaat     3600

gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct     3660

gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt     3720

ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa     3780

ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt     3840

tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac     3900

tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgaggcga aatacgcgat     3960

cgctgttaaa aggacaatta caaacaggaa tcgagtgcaa ccggcgcagg aacactgcca     4020

gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aacgctgttt     4080

ttccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga     4140

tggtcggaag tggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat     4200

cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat     4260

acaagcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat     4320

ataaatcagc atccatgttg gaatttaatc gcggcctcga cgtttcccgt tgaatatggc     4380

tcatattctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg     4440

gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggtcagtgtt acaaccaatt     4500

aaccaattct gaacattatc gcgagcccat ttatacctga atatggctca taacacccct     4560

tgtttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg     4620

aaacgccgta gcgccgatgg tagtgtgggg actccccatg cgagagtagg gaactgccag     4680

gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgcccgg gctaattagg     4740

gggtgtcgcc cttattcgac tctatagtga agttcctatt ctctagaaag tataggaact     4800

tctgaagtgg ggacgctttc cggcatcttc cagaccacag tatatccatc cgcctcctgt     4860

tg                                                                    4862


<210> 4
<211> 5513
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5513
<223> /organism="Artificial Sequence"
      /note="Sequence containing the DAK1-gene of S. cerevisiae under
      control of the TPI1-promoter and the ENO1-terminator"
      /mol_type="unassigned DNA"

<400> 4
gtgcgacacc taactacata gtgtttaaag attacggata tttaacttac ttagaataat       60

gccatttttt tgagttataa taatcctacg ttagtgtgag cgggatttaa actgtgagga      120

ccttaataca ttcagacact tctgcggtat caccctactt attcccttcg agattatatc      180

taggaaccca tcaggttggt ggaagattac ccgttctaag acttttcagc ttcctctatt      240

gatgttacac ctggacaccc cttttctggc atccagtttt taatcttcag tggcatgtga      300

gattctccga aattaattaa agcaatcaca caattctctc ggataccacc tcggttgaaa      360

ctgacaggtg gtttgttacg catgctaatg caaaggagcc tatatacctt tggctcggct      420

gctgtaacag ggaatataaa gggcagcata atttaggagt ttagtgaact tgcaacattt      480

actattttcc cttcttacgt aaatattttt ctttttaatt ctaaatcaat ctttttcaat      540

tttttgtttg tattcttttc ttgcttaaat ctataactac aaaaaacaca tacataaact      600

aaaaatgtcc gctaaatctt tcgaagttac cgacccagtc aactcttctt tgaagggttt      660

tgctttggcc aacccatcca ttactttggt cccagaagaa aagatcttat tcagaaagac      720

tgactctgac aaaattgctt tgatctccgg tggtggttcc ggtcacgaac caacccacgc      780

tggtttcatc ggtaagggta tgttgtccgg tgctgtcgtt ggtgaaatct ttgcttctcc      840

atccaccaag caaatcttga atgctatcag attagtcaac gaaaacgctt ctggtgtctt      900

gttgattgtc aagaactaca ctggtgacgt cttgcatttc ggtttatctg ctgaaagagc      960

tagagctttg ggtattaact gtagagttgc cgtcatcggt gacgatgttg ctgtcggtcg     1020

tgaaaagggt ggtatggttg gtagacgtgc tttggctggt actgtcttgg ttcacaagat     1080

tgttggtgct ttcgctgaag aatactcctc caagtacggt ttagatggta ctgctaaggt     1140

tgccaagatc atcaacgaca acttggttac catcggttct tctttggacc actgtaaggt     1200

tccaggtaga aagttcgaat ctgaattgaa cgaaaagcaa atggaattgg gtatgggtat     1260

ccacaacgaa ccaggtgtta aggtcttgga cccaattcca tccactgaag atttgatttc     1320

caaatacatg ttgccaaagt tgctagaccc aaacgacaag gacagagctt tcgttaagtt     1380

cgatgaagat gacgaagttg ttttgttggt caacaacttg ggtggtgttt ctaacttcgt     1440

catctcttct attacctcca agaccaccga tttcttaaag gaaaactaca acatcactcc     1500

agtccaaacc attgccggta ctttgatgac ctctttcaac ggtaacggtt tctccatcac     1560

cttgttgaat gccaccaaag ctaccaaggc tttgcaatct gatttcgaag aaatcaaatc     1620

cgtcttagat ttgttgaacg ccttcaccaa cgccccaggt tggccaattg ctgacttcga     1680

aaagacctct gctccatctg ttaacgatga cttgttgcac aacgaagtta ctgccaaggc     1740

cgtcggtact tacgatttcg acaaattcgc tgaatggatg aagtctggtg ctgaacaagt     1800

catcaaatct gaaccacaca tcactgaatt ggacaaccaa gttggtgatg gtgactgtgg     1860

ttacactttg gttgctggtg tcaagggtat cactgaaaac ttggacaaat tgtccaagga     1920

ctctttgtct caagctgttg ctcaaatttc tgatttcatt gaaggttcca tgggtggtac     1980

ttctggtggt ttgtactcca tcttgttgtc tggtttctcc cacggtttga tccaagtttg     2040

taagtccaag gatgaacctg tcaccaagga aattgttgcc aagtctctag gtattgcttt     2100

ggacacttta tacaagtaca ccaaggccag aaagggttct tccaccatga tcgatgcttt     2160

ggaaccattt gtcaaggaat tcactgcttc taaggacttc aacaaggctg ttaaggctgc     2220

tgaagaaggt gccaagtcca ctgctacttt cgaagctaag ttcggtagag cttcttacgt     2280

tggtgactct tctcaagttg aagatccagg tgctgttggt ttatgtgaat tcttgaaggg     2340

tgtccaatct gcgctttaaa agcttttgat taagccttct agtccaaaaa acacgttttt     2400

ttgtcattta tttcattttc ttagaatagt ttagtttatt cattttatag tcacgaatgt     2460

tttatgattc tatatagggt tgcaaacaag catttttcat tttatgttaa aacaatttca     2520

ggtttacctt ttattctgct tgtggtgacg cgtgtatccg cccgctcttt tggtcaccca     2580

tgtatttaat tgcataaata attcttaaaa gtggagctag tctatttcta tttacatacc     2640

tctcatttct catttcctcc cctcagaaag cctgtatgcg aagccacaat cctttccaac     2700

agaccatact aagtaaaatg aagtgaagtt cctatacttt ctagagaata ggaacttcta     2760

tagtgagtcg aataagggcg acacaaaatt tattctaaat gcataataaa tactgataac     2820

atcttatagt ttgtattata ttttgtatta tcgttgacat gtataatttt gatatcaaaa     2880

actgattttc cctttattat tttcgagatt tattttctta attctcttta acaaactaga     2940

aatattgtat atacaaaaaa tcataaataa tagatgaata gtttaattat aggtgttcat     3000

caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct tttttctcat ttataaggtt     3060

aaataattct catatatcaa gcaaagtgac aggcgccctt aaatattctg acaaatgctc     3120

tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga     3180

ttaacgatta ctcgttatca gaaccgccca gggggcccga gcttaagact ggccgtcgtt     3240

ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca tccgtcaggg gccttctgct     3300

tagtttgatg cctggcagtt ccctactctc gccttccgct tcctcgctca ctgactcgct     3360

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     3420

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     3480

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     3540

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     3600

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     3660

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     3720

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     3780

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     3840

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     3900

aggcggtgct acagagttct tgaagtggtg ggctaactac ggctacacta gaagaacagt     3960

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     4020

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     4080

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     4140

gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg tcatgagctt gcgccgtccc     4200

gtcaagtcag cgtaatgctc tgcttttaga aaaactcatc gagcatcaaa tgaaactgca     4260

atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag     4320

gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc     4380

cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa     4440

gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt     4500

ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa     4560

ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa     4620

aaggacaatt acaaacagga atcgagtgca accggcgcag gaacactgcc agcgcatcaa     4680

caatattttc acctgaatca ggatattctt ctaatacctg gaacgctgtt tttccgggga     4740

tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa     4800

gtggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa     4860

cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat     4920

agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag     4980

catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcatattct     5040

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat     5100

ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat taaccaattc     5160

tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc ttgtttgcct     5220

ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt     5280

agcgccgatg gtagtgtggg gactccccat gcgagagtag ggaactgcca ggcatcaaat     5340

aaaacgaaag gctcagtcga aagactgggc ctttcgcccg ggctaattag ggggtgtcgc     5400

ccttattcga ctctatagtg aagttcctat tctctagaaa gtataggaac ttctgaagtg     5460

gggaacgttg tccaggtttg tatccacgtg tgtccgttcc gccaatattc cgc            5513


<210> 5
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Forward primer expression cassette 1 (con5-f)"
      /mol_type="unassigned DNA"

<400> 5
aagcgacttc caatcgcttt gc                                             22


<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Reverse primer expression cassette 1 (conA-r)"
      /mol_type="unassigned DNA"

<400> 6
aaagcaaagg aaggagagaa c                                              21


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Forward primer expression cassette 2 (conB-f)"
      /mol_type="unassigned DNA"

<400> 7
cggatcgatg tacacaaccg                                                20


<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /organism="Artificial Sequence"
      /note="Reverse primer expression cassette 2 (conC-r)"
      /mol_type="unassigned DNA"

<400> 8
caacaggagg cggatggata tac                                            23


<210> 9
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Forward primer expression cassette 3 (conC-f)"
      /mol_type="unassigned DNA"

<400> 9
acgctttccg gcatcttcca g                                              21


<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Reverse primer expression cassette 3 (conD-r)"
      /mol_type="unassigned DNA"

<400> 10
gcggaatatt ggcggaacgg                                                20


<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Forward primer expression cassette 4 (conD-f)"
      /mol_type="unassigned DNA"

<400> 11
aacgttgtcc aggtttgtat cc                                             22


<210> 12
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /organism="Artificial Sequence"
      /note="Reverse primer expression cassette 4 (con3-r)"
      /mol_type="unassigned DNA"

<400> 12
acttagtatg gtctgttgga aagg                                           24


<210> 13
<211> 1674
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1674
<223> /organism="Artificial Sequence"
      /note="Sequence of the kanMX marker and flanking regions"
      /mol_type="unassigned DNA"

<400> 13
ttgcccatcg aacgtacaag tactcctctg ttctctcctt cctttgcttt cttcgtacgc      60

tgcaggtcga cgaattctac cgttcgtata atgtatgcta tacgaagtta tagatctgtt     120

tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc     180

cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag     240

cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga     300

agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac     360

gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca     420

ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc     480

acatccgaac ataaacaacc atgggtaagg aaaagactca cgtttcgagg ccgcgattaa     540

attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat     600

caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac     660

atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga     720

cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt     780

tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa tatcctgatt     840

caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg     900

tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa     960

tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg    1020

aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc    1080

atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg    1140

atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc    1200

tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc    1260

ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagtactga    1320

caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt    1380

tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg    1440

cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct    1500

ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtgtcga aaacgagctc    1560

ataacttcgt ataatgtatg ctatacgaac ggtagaattc gatatcagat ccactagtgg    1620

cctacggatc gatgtacaca accgactgca cccaaacgaa cacaaatctt agca          1674


<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Forward primer marker (conA-f)"
      /mol_type="unassigned DNA"

<400> 14
ttgcccatcg aacgtacaag                                                20


<210> 15
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /organism="Artificial Sequence"
      /note="Reverse primer marker (conB-r)"
      /mol_type="unassigned DNA"

<400> 15
tgctaagatt tgtgttcgtt tgg                                            23


<210> 16
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /organism="Artificial Sequence"
      /note="Forward primer 5â INT1 fragment (INT5-f)"
      /mol_type="unassigned DNA"

<400> 16
cggcattatt gtgtatggc                                                 19


<210> 17
<211> 73
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..73
<223> /organism="Artificial Sequence"
      /note="Reverse primer 5â INT1 fragment (INT5-r)"
      /mol_type="unassigned DNA"

<400> 17
aaacgcctgt gggtgtggta ctggatatgc aaagcgattg gaagtcgctt agggtttcaa     60

agatccatac ttc                                                        73


<210> 18
<211> 90
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..90
<223> /organism="Artificial Sequence"
      /note="Forward primer 3â INT1 fragment (INT3-f)"
      /mol_type="unassigned DNA"

<400> 18
agaaagcctg tatgcgaagc cacaatcctt tccaacagac catactaagt attttatttt     60

acttttttta gaatgacctg ttcccgacac                                      90


<210> 19
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /organism="Artificial Sequence"
      /note="Reverse primer 3â INT1 fragment (INT3-r)"
      /mol_type="unassigned DNA"

<400> 19
cacaagctta ttcttccaaa aatc                                           24


<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Forward primer for amplification of the 5â-INT59 flank"
      /mol_type="unassigned DNA"

<400> 20
cattatatcg aggaaagccc                                                20


<210> 21
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /organism="Artificial Sequence"
      /note="Reverse primer for amplification of the 5â-INT59 flank"
      /mol_type="unassigned DNA"

<400> 21
aaacgcctgt gggtgtggta ctggatatgc aaagcgattg gaagtcgctt agaaagagac     60

acaaaactac                                                            70


<210> 22
<211> 73
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..73
<223> /organism="Artificial Sequence"
      /note="Forward primer for amplification of the 3â-INT59 flank"
      /mol_type="unassigned DNA"

<400> 22
gaaaccttcg aatccagcca gcatgtcgac acccacaaga tgtagtgcac tcttggcggc     60

cgtacgcata tag                                                        73


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Reverse primer for amplification of the 3â-INT59 flank"
      /mol_type="unassigned DNA"

<400> 23
cgcacaccac gaggtgaagg                                                20


<210> 24
<211> 4118
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4118
<223> /organism="Artificial Sequence"
      /note="Sequence containing the natMX marker"
      /mol_type="unassigned DNA"

<400> 24
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggaaa taaccacaaa catccttccc     1260

atatgctcgg tcgtgcttgt tgtacctgtg ctaccgttcg tataatgtat gctatacgaa     1320

gttatgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac     1380

agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata     1440

catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa     1500

aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg     1560

aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg     1620

ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc     1680

gaacataaac aacaatgggt accactcttg acgacacggc ttaccggtac cgcaccagtg     1740

tcccggggga cgccgaggcc atcgaggcac tggatgggtc cttcaccacc gacaccgtct     1800

tccgcgtcac cgccaccggg gacggcttca ccctgcggga ggtgccggtg gacccgcccc     1860

tgaccaaggt gttccccgac gacgaatcgg acgacgaatc ggacgacggg gaggacggcg     1920

acccggactc ccggacgttc gtcgcgtacg gggacgacgg cgacctggcg ggcttcgtgg     1980

tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga ggacatcgag gtcgccccgg     2040

agcaccgggg gcacggggtc gggcgcgcgt tgatggggct cgcgacggag ttcgcccgcg     2100

agcggggcgc cgggcacctc tggctggagg tcaccaacgt caacgcaccg gcgatccacg     2160

cgtaccggcg gatggggttc accctctgcg gcctggacac cgccctgtac gacggcaccg     2220

cctcggacgg cgagcaggcg ctctacatga gcatgccctg cccctaaatc agtactgaca     2280

ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg     2340

ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc     2400

cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg     2460

tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctcat     2520

aacttcgtat aatgtatgct atacgaacgg tacctcgaaa ccttcgaatc cagccagcat     2580

gtcgacaccc acaagatgta gtgcacaaaa tgaagtgaag ttcctatact ttctagagaa     2640

taggaacttc tatagtgagt cgaataaggg cgacacaaaa tttattctaa atgcataata     2700

aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt     2760

ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct taattctctt     2820

taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt     2880

ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc     2940

atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc     3000

tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac     3060

gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga     3120

ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc catccgtcag     3180

gggccttctg cttagtttga tgcctggcag ttccctactc tcgccttccg cttcctcgct     3240

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     3300

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     3360

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     3420

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     3480

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     3540

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     3600

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     3660

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     3720

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     3780

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tgggctaact acggctacac     3840

tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     3900

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     3960

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     4020

gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt aagggatttt ggtcatgagc     4080

ttgcgccgtc ccgtcaagtc agcgtaatgc tctgcttt                             4118


<210> 25
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /organism="Artificial Sequence"
      /note="Forward primer for the amplification of the natMX marker"
      /mol_type="unassigned DNA"

<400> 25
aaataaccac aaacatcctt ccc                                            23


<210> 26
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /organism="Artificial Sequence"
      /note="Reverse primer for the amplification of the natMX marker"
      /mol_type="unassigned DNA"

<400> 26
gtgcactaca tcttgtgggt gtc                                            23


<210> 27
<211> 598
<212> PRT
<213> Schizosaccharomyces pombe

<220> 
<223> Protein sequence of the glycerol transporter from S. pombe

<400> 27
Met Ser Val Pro Leu Arg Phe Ser Thr Pro Ser Ser Ser Pro Ser Ala 
1               5                   10                  15      
Ser Asp Asn Glu Ser Val His Asp Asp Gly Pro Thr Thr Glu Leu Asp 
            20                  25                  30          
Thr Phe Asn Thr Thr Asp Val Pro Arg Arg Val Asn Thr Thr Lys Ala 
        35                  40                  45              
Arg Gln Met Arg Pro Lys Asn Thr Leu Lys Val Ala Phe Ser Ser Pro 
    50                  55                  60                  
Asn Leu Lys Gly Leu Asp Asn Thr Ala Asp Ser Asp Ser Gln Pro Trp 
65                  70                  75                  80  
Leu Gly Gly Tyr Leu Ala Gly Arg Leu Glu Asp Ile Ser Gly Gln Ser 
                85                  90                  95      
Arg Arg Asn Tyr Val Asp Pro Tyr Tyr Glu Glu Leu Asn Ala Gly Arg 
            100                 105                 110         
Arg Pro Asn Lys Pro Val Trp Ser Leu Asn Gly Pro Leu Pro His Val 
        115                 120                 125             
Leu Gly Asn Ser Val Val Glu Lys Ile Ser Gln Lys Asn Gln Glu Ala 
    130                 135                 140                 
Arg Ser Arg Ala Asn Ser Arg Val Asn Ser Arg Ala Asn Ser Arg Ala 
145                 150                 155                 160 
Asn Ser Ser Val Ser Leu Ala Gly Met Asp Gly Ser Pro Asn Trp Lys 
                165                 170                 175     
Arg Lys Met Lys Ser Ala Val Phe Gly Ser Arg Val Lys Leu Asn Asp 
            180                 185                 190         
Glu Glu Ala Gln Leu Pro Arg Asn Lys Ser Ser Val Ser Ile Ala Glu 
        195                 200                 205             
Gln Ala Ala Ser Arg Pro Lys Val Ser Phe Ser Leu Gln Ser Ser Arg 
    210                 215                 220                 
Gln Pro Ser Ile Ala Glu Glu Gln Pro Gln Thr Gln Arg Lys Ser Ser 
225                 230                 235                 240 
Ala Ile Thr Val Glu His Ala Glu Asn Ala Glu Pro Glu Thr Pro Arg 
                245                 250                 255     
Asn Asn Val Ser Phe Ser Arg Lys Pro Ser Ile Ala Glu Gln Asp Ser 
            260                 265                 270         
Ser Gln Asp Ile Thr Met Pro Pro Asn Glu Ile Ile Ala Glu Glu Ser 
        275                 280                 285             
Leu Asp Ser Gly Ser Asp Thr Glu Thr Leu Tyr Leu Asn Tyr Trp Cys 
    290                 295                 300                 
Lys Ile Arg His Phe Phe Arg Glu Gly Phe Ala Glu Phe Leu Gly Thr 
305                 310                 315                 320 
Leu Val Leu Val Val Phe Gly Val Gly Ser Asn Leu Gln Ala Thr Val 
                325                 330                 335     
Thr Asn Gly Ala Gly Gly Ser Phe Glu Ser Leu Ser Phe Ala Trp Gly 
            340                 345                 350         
Phe Gly Cys Met Leu Gly Val Tyr Ile Ala Gly Gly Ile Ser Gly Gly 
        355                 360                 365             
His Val Asn Pro Ala Val Thr Ile Ser Leu Ala Ile Phe Arg Lys Phe 
    370                 375                 380                 
Pro Trp Tyr Lys Val Pro Ile Tyr Ile Phe Phe Gln Ile Trp Gly Ala 
385                 390                 395                 400 
Phe Phe Gly Gly Ala Leu Ala Tyr Gly Tyr His Trp Ser Ser Ile Thr 
                405                 410                 415     
Glu Phe Glu Gly Gly Lys Asp Ile Arg Thr Pro Ala Thr Gly Gly Cys 
            420                 425                 430         
Leu Tyr Thr Asn Pro Lys Pro Tyr Val Thr Trp Arg Asn Ala Phe Phe 
        435                 440                 445             
Asp Glu Phe Ile Gly Thr Ala Val Leu Val Gly Cys Leu Phe Ala Ile 
    450                 455                 460                 
Leu Asp Asp Thr Asn Ser Pro Pro Thr Gln Gly Met Thr Ala Phe Ile 
465                 470                 475                 480 
Val Gly Leu Leu Ile Ala Ala Ile Gly Met Ala Leu Gly Tyr Gln Thr 
                485                 490                 495     
Ser Phe Thr Leu Asn Pro Ala Arg Asp Leu Gly Pro Arg Met Phe Ala 
            500                 505                 510         
Trp Trp Ile Gly Tyr Gly Pro His Ser Phe His Leu Tyr His Trp Trp 
        515                 520                 525             
Trp Thr Trp Gly Ala Trp Gly Gly Thr Ile Gly Gly Gly Ile Ala Gly 
    530                 535                 540                 
Gly Leu Ile Tyr Asp Leu Val Ile Phe Thr Gly Pro Glu Ser Pro Leu 
545                 550                 555                 560 
Asn Tyr Pro Asp Asn Gly Phe Ile Asp Lys Lys Val His Gln Ile Thr 
                565                 570                 575     
Ala Lys Phe Glu Lys Glu Glu Glu Val Glu Asn Leu Glu Lys Thr Asp 
            580                 585                 590         
Ser Pro Ile Glu Asn Asn 
        595             

<210> 28
<211> 258
<212> PRT
<213> Plasmodium falciparum

<220> 
<223> Protein sequence of the glycerol transporter from Plasmodium
      falciparum

<400> 28
Met His Met Leu Phe Tyr Lys Ser Tyr Val Arg Glu Phe Ile Gly Glu 
1               5                   10                  15      
Phe Leu Gly Thr Phe Val Leu Met Phe Leu Gly Glu Gly Ala Thr Ala 
            20                  25                  30          
Asn Phe His Thr Thr Gly Leu Ser Gly Asp Trp Tyr Lys Leu Cys Leu 
        35                  40                  45              
Gly Trp Gly Leu Ala Val Phe Phe Gly Ile Leu Val Ser Ala Lys Leu 
    50                  55                  60                  
Ser Gly Ala His Leu Asn Leu Ala Val Ser Ile Gly Leu Ser Ser Ile 
65                  70                  75                  80  
Asn Lys Phe Asp Leu Lys Lys Ile Pro Val Tyr Phe Phe Ala Gln Leu 
                85                  90                  95      
Leu Gly Ala Phe Val Gly Thr Ser Thr Val Tyr Gly Leu Tyr His Gly 
            100                 105                 110         
Phe Ile Ser Asn Ser Lys Ile Pro Gln Phe Ala Trp Glu Thr Ser Arg 
        115                 120                 125             
Asn Pro Ser Ile Ser Leu Thr Gly Ala Phe Phe Asn Glu Leu Ile Leu 
    130                 135                 140                 
Thr Gly Ile Leu Leu Leu Val Ile Leu Val Val Val Asp Glu Asn Ile 
145                 150                 155                 160 
Cys Gly Lys Phe His Ile Leu Lys Leu Ser Ser Val Val Gly Leu Ile 
                165                 170                 175     
Ile Leu Cys Ile Gly Ile Thr Phe Gly Gly Asn Thr Gly Phe Ala Leu 
            180                 185                 190         
Asn Pro Ser Arg Asp Leu Gly Ser Arg Phe Leu Ser Leu Ile Ala Tyr 
        195                 200                 205             
Gly Lys Asp Thr Phe Thr Lys Asp Asn Phe Tyr Phe Trp Val Pro Leu 
    210                 215                 220                 
Val Ala Pro Cys Val Gly Ser Val Val Phe Cys Gln Phe Tyr Asp Lys 
225                 230                 235                 240 
Val Ile Cys Pro Leu Val Asp Leu Ala Asn Asn Glu Lys Asp Gly Val 
                245                 250                 255     
Asp Leu 
        

<210> 29
<211> 291
<212> PRT
<213> Danio rerio

<220> 
<223> Protein sequence of the glycerol transporter from Danio rerio;

<400> 29
Met Glu Tyr Leu Glu Asn Ile Arg Asn Leu Arg Gly Arg Cys Val Leu 
1               5                   10                  15      
Arg Arg Asp Ile Ile Arg Glu Phe Leu Ala Glu Leu Leu Gly Thr Phe 
            20                  25                  30          
Val Leu Ile Leu Phe Gly Cys Gly Ser Val Ala Gln Thr Val Leu Ser 
        35                  40                  45              
Arg Glu Ala Lys Gly Gln Leu Leu Thr Ile His Phe Gly Phe Thr Leu 
    50                  55                  60                  
Gly Val Met Leu Ala Val Tyr Met Ala Gly Gly Val Ser Gly Gly His 
65                  70                  75                  80  
Val Asn Pro Ala Val Ser Leu Ala Met Val Val Leu Arg Lys Leu Pro 
                85                  90                  95      
Leu Lys Lys Phe Pro Val Tyr Val Leu Ala Gln Phe Leu Gly Ala Phe 
            100                 105                 110         
Phe Gly Ser Cys Ala Val Tyr Cys Leu Tyr Tyr Asp Ala Phe Thr Glu 
        115                 120                 125             
Phe Ala Asn Gly Glu Leu Ala Val Thr Gly Pro Asn Val Thr Ala Gly 
    130                 135                 140                 
Ile Phe Ala Ser Tyr Pro Arg Glu Gly Leu Ser Leu Leu Asn Gly Phe 
145                 150                 155                 160 
Ile Asp Gln Val Ile Gly Ala Gly Ala Leu Val Leu Cys Ile Leu Ala 
                165                 170                 175     
Val Val Asp Lys Lys Asn Ile Gly Ala Pro Lys Gly Met Glu Pro Leu 
            180                 185                 190         
Leu Val Gly Leu Ser Ile Leu Ala Ile Gly Val Ser Met Ala Leu Asn 
        195                 200                 205             
Cys Gly Tyr Pro Ile Asn Pro Ala Arg Asp Leu Gly Pro Arg Leu Phe 
    210                 215                 220                 
Thr Ala Ile Ala Gly Trp Gly Leu Thr Val Phe Ser Ala Gly Asn Gly 
225                 230                 235                 240 
Trp Trp Trp Val Pro Val Val Gly Pro Met Val Gly Gly Val Val Gly 
                245                 250                 255     
Ala Ala Ile Tyr Phe Leu Met Ile Glu Met His His Pro Glu Asn Asp 
            260                 265                 270         
Lys Asn Leu Glu Asp Asp Asn Ser Leu Lys Asp Lys Tyr Glu Leu Asn 
        275                 280                 285             
Thr Val Asn 
    290     

<210> 30
<211> 292
<212> PRT
<213> Xenopus (Silurana) tropicalis

<220> 
<223> Protein sequence of the glycerol transporter from Xenopus
      tropicalis;

<400> 30
Met Gly Arg Gln Lys Asp Phe Val Asn Lys Cys Asn Gln Met Leu Arg 
1               5                   10                  15      
Leu Arg Asn Lys Leu Leu Arg Gln Ala Leu Ser Glu Cys Leu Gly Thr 
            20                  25                  30          
Leu Ile Leu Val Met Phe Gly Cys Gly Ser Val Ala Gln Val Val Leu 
        35                  40                  45              
Ser Lys Gly Ser His Gly Gln Phe Leu Thr Val Asn Leu Ala Phe Gly 
    50                  55                  60                  
Phe Ala Val Met Leu Gly Ile Leu Ile Ser Gly Gln Val Ser Gly Gly 
65                  70                  75                  80  
His Leu Asn Pro Ala Val Thr Phe Ala Leu Cys Ile Leu Ala Arg Glu 
                85                  90                  95      
Pro Trp Val Lys Phe Pro Val Tyr Ser Ile Ala Gln Thr Leu Gly Ala 
            100                 105                 110         
Phe Leu Gly Ala Gly Ile Ile Tyr Gly Leu Tyr Tyr Asp Ala Ile Trp 
        115                 120                 125             
Tyr Phe Ala Asn Asp Gln Leu Tyr Val Thr Gly Glu Asn Gly Thr Ala 
    130                 135                 140                 
Gly Ile Phe Thr Thr Phe Pro Ser Asp His Leu Thr Leu Met Asn Gly 
145                 150                 155                 160 
Phe Phe Asp Gln Phe Ile Gly Thr Ala Ala Leu Val Val Cys Val Leu 
                165                 170                 175     
Ala Ile Val Asp Pro Asn Asn Asn Pro Ile Pro Arg Gly Leu Glu Ala 
            180                 185                 190         
Phe Thr Val Gly Phe Val Val Leu Val Ile Gly Thr Ser Met Gly Phe 
        195                 200                 205             
Asn Ser Gly Tyr Ala Val Asn Pro Ala Arg Asp Phe Gly Pro Arg Leu 
    210                 215                 220                 
Phe Thr Ser Leu Ala Gly Trp Gly Thr Glu Val Phe Trp Ala Gly Asn 
225                 230                 235                 240 
Gln Trp Trp Trp Val Pro Ile Val Ser Pro Leu Leu Gly Ala Phe Ala 
                245                 250                 255     
Gly Val Leu Val Tyr Gln Leu Met Ile Gly Cys His Ile Glu Pro Val 
            260                 265                 270         
Pro Gln Ser Thr Glu Gln Glu Asn Ile Lys Leu Ala Asp Val Lys Pro 
        275                 280                 285             
Lys Asp Arg Ile 
    290         

<210> 31
<211> 592
<212> PRT
<213> Zygosaccharomyces rouxii

<220> 
<223> Protein sequence of the glycerol transporter from Z. rouxii;

<400> 31
Met Gly Lys Arg Thr Gln Gly Phe Met Asp Tyr Val Phe Ser Arg Thr 
1               5                   10                  15      
Ser Thr Ala Gly Leu Lys Gly Ala Arg Leu Arg Tyr Thr Ala Ala Ala 
            20                  25                  30          
Val Ala Val Ile Gly Phe Ala Leu Phe Gly Tyr Asp Gln Gly Leu Met 
        35                  40                  45              
Ser Gly Leu Ile Thr Gly Asp Gln Phe Asn Lys Glu Phe Pro Pro Thr 
    50                  55                  60                  
Lys Ser Asn Gly Asp Asn Asp Arg Tyr Ala Ser Val Ile Gln Gly Ala 
65                  70                  75                  80  
Val Thr Ala Cys Tyr Glu Ile Gly Cys Phe Phe Gly Ser Leu Phe Val 
                85                  90                  95      
Leu Phe Phe Gly Asp Ala Ile Gly Arg Lys Pro Leu Ile Ile Phe Gly 
            100                 105                 110         
Ala Ile Ile Val Ile Ile Gly Thr Val Ile Ser Thr Ala Pro Phe His 
        115                 120                 125             
His Ala Trp Gly Leu Gly Gln Phe Val Val Gly Arg Val Ile Thr Gly 
    130                 135                 140                 
Val Gly Thr Gly Phe Asn Thr Ser Thr Ile Pro Val Trp Gln Ser Glu 
145                 150                 155                 160 
Met Thr Lys Pro Asn Ile Arg Gly Ala Met Ile Asn Leu Asp Gly Ser 
                165                 170                 175     
Val Ile Ala Phe Gly Thr Met Ile Ala Tyr Trp Leu Asp Phe Gly Phe 
            180                 185                 190         
Ser Phe Ile Asn Ser Ser Val Gln Trp Arg Phe Pro Val Ser Val Gln 
        195                 200                 205             
Ile Ile Phe Ala Leu Val Leu Leu Phe Gly Ile Val Arg Met Pro Glu 
    210                 215                 220                 
Ser Pro Arg Trp Leu Met Ala Lys Lys Arg Pro Ala Glu Ala Arg Tyr 
225                 230                 235                 240 
Val Leu Ala Cys Leu Asn Asp Leu Pro Glu Asn Asp Asp Ala Ile Leu 
                245                 250                 255     
Ala Glu Met Thr Ser Leu His Glu Ala Val Asn Arg Ser Ser Asn Gln 
            260                 265                 270         
Lys Ser Gln Met Lys Ser Leu Phe Ser Met Gly Lys Gln Gln Asn Phe 
        275                 280                 285             
Ser Arg Ala Leu Ile Ala Ser Ser Thr Gln Phe Phe Gln Gln Phe Thr 
    290                 295                 300                 
Gly Cys Asn Ala Ala Ile Tyr Tyr Ser Thr Val Leu Phe Gln Thr Thr 
305                 310                 315                 320 
Val Gln Leu Asp Arg Leu Leu Ala Met Ile Leu Gly Gly Val Phe Ala 
                325                 330                 335     
Thr Val Tyr Thr Leu Ser Thr Leu Pro Ser Phe Tyr Leu Val Glu Lys 
            340                 345                 350         
Val Gly Arg Arg Lys Met Phe Phe Phe Gly Ala Leu Gly Gln Gly Ile 
        355                 360                 365             
Ser Phe Ile Ile Thr Phe Ala Cys Leu Val Asn Pro Thr Lys Gln Asn 
    370                 375                 380                 
Ala Lys Gly Ala Ala Val Gly Leu Tyr Leu Phe Ile Ile Cys Phe Gly 
385                 390                 395                 400 
Leu Ala Ile Leu Glu Leu Pro Trp Ile Tyr Pro Pro Glu Ile Ala Ser 
                405                 410                 415     
Met Arg Val Arg Ala Ala Thr Asn Ala Met Ser Thr Cys Thr Asn Trp 
            420                 425                 430         
Val Thr Asn Phe Ala Val Val Met Phe Thr Pro Val Phe Ile Gln Thr 
        435                 440                 445             
Ser Gln Trp Gly Cys Tyr Leu Phe Phe Ala Val Met Asn Phe Ile Tyr 
    450                 455                 460                 
Leu Pro Val Ile Phe Phe Phe Tyr Pro Glu Thr Ala Gly Arg Ser Leu 
465                 470                 475                 480 
Glu Glu Ile Asp Ile Ile Phe Ala Lys Ala His Val Asp Gly Thr Leu 
                485                 490                 495     
Pro Trp Met Val Ala His Arg Leu Pro Lys Leu Ser Met Thr Glu Val 
            500                 505                 510         
Glu Asp Tyr Ser Gln Ser Leu Gly Leu His Asp Asp Glu Asn Glu Lys 
        515                 520                 525             
Glu Glu Tyr Asp Glu Lys Glu Ala Glu Ala Asn Ala Ala Leu Phe Gln 
    530                 535                 540                 
Val Glu Thr Ser Ser Lys Ser Pro Ser Ser Asn Arg Lys Asp Asp Asp 
545                 550                 555                 560 
Ala Pro Ile Glu His Asn Glu Val Gln Glu Ser Asn Asp Asn Ser Ser 
                565                 570                 575     
Asn Ser Ser Asn Val Glu Ala Pro Ile Pro Val His His Asn Asp Pro 
            580                 585                 590         



<210> 32
<211> 5555
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5555
<223> /organism="Artificial Sequence"
      /note="Plasmid containing the expression cassette of the S. pombe
      glycerol transporter;"
      /mol_type="unassigned DNA"

<400> 32
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggaag cgacttccaa tcgctttgca     1260

tatccagtac cacacccaca ggcgtttgtg cgtgtcgacg ctgcgggtat agaaagggtt     1320

ctttactcta tagtacctcc tcgctcagca tctgcttctt cccaaagatg aacgcggcgt     1380

tatgtcacta acgacgtgca ccaacttgcg gaaagtggaa tcccgttcca aaactggcat     1440

ccactaattg atacatctac acaccgcacg ccttttttct gaagcccact ttcgtggact     1500

ttgccatatg caaaattcat gaagtgtgat accaagtcag catacacctc actagggtag     1560

tttctttggt tgtattgatc atttggttca tcgtggttca ttaatttttt ttctccattg     1620

ctttctggct ttgatcttac tatcatttgg atttttgtcg aaggttgtag aattgtatgt     1680

gacaagtggc accaagcata tataaaaaaa aaaagcatta tcttcctacc agagttgatt     1740

gttaaaaacg tatttatagc aaacgcaatt gtaattaatt cttattttgt atcttttctt     1800

cccttgtctc aatcttttat ttttatttta tttttctttt cttagtttct ttcataacac     1860

caagcaacta atactataac atacaataat aatgtccgtt ccattgagat tctccacccc     1920

atcttcttct ccatctgctt ctgacaacga atctgttcac gatgacggtc caaccactga     1980

attggacacc tttaacacta ccgatgtccc aagaagagtc aacactacca aggctagaca     2040

aatgcgtcca aagaacactt tgaaggttgc tttctcctcc ccaaacttga aaggtttgga     2100

caacactgct gactctgatt ctcaaccttg gttaggtggt tacttggctg gtagattgga     2160

agatatctcc ggtcaatcca gaagaaacta cgttgaccca tactacgaag aattgaacgc     2220

tggtcgtaga ccaaacaagc cagtctggtc cttgaatggt ccattgcctc acgtcttagg     2280

taactccgtt gttgaaaaga tttcccaaaa gaaccaagaa gctagatcca gagccaactc     2340

tcgtgttaac tcccgtgcca actccagagc taactcttct gtttccttgg ccggtatgga     2400

cggttctcca aactggaaga gaaagatgaa atctgccgtt ttcggttcca gagtcaaatt     2460

gaacgacgaa gaagctcaat tgccaagaaa caaatcttct gtctctattg ctgaacaagc     2520

tgcttccaga ccaaaagtct ctttctcctt gcaatcttcc agacaaccat ccattgccga     2580

agaacaacca caaacccaaa gaaagtcctc tgctatcact gttgaacatg ctgaaaacgc     2640

tgaaccagaa actccacgta acaacgtttc tttctccaga aagccttcca ttgctgagca     2700

agattcttcc caagatatca ccatgccacc aaacgaaatc attgccgagg aatctttgga     2760

ctctggttct gacaccgaaa ctttgtactt gaactactgg tgtaagatca gacacttctt     2820

cagagaaggt ttcgctgaat tcttgggtac tttggtctta gttgttttcg gtgttggttc     2880

caacttgcaa gctaccgtca ccaacggtgc tggtggctct ttcgaatctt tatctttcgc     2940

ttggggtttc ggttgtatgt tgggtgtcta cattgctggt ggtatttctg gtggtcacgt     3000

taacccagct gtcaccatct ctttggccat tttcagaaag ttcccatggt acaaggtccc     3060

aatctacatc tttttccaaa tctggggtgc cttcttcggt ggtgctttgg cttacggtta     3120

tcactggtcc tctatcactg aatttgaagg tggtaaggac atcagaactc cagctaccgg     3180

tggttgtcta tacaccaacc caaagccata cgttacctgg agaaacgctt tcttcgatga     3240

atttatcggt actgctgtct tggttggttg tttgttcgcc attttggacg acaccaactc     3300

tccaccaact caaggtatga ccgctttcat cgtcggtttg ttgattgctg ccattggtat     3360

ggctctaggt taccaaactt ctttcacttt aaacccagct agagatttgg gtccaagaat     3420

gtttgcttgg tggattggtt acggtcctca ctctttccat ttgtaccact ggtggtggac     3480

ttggggtgct tggggtggta ctatcggtgg tggtatcgct ggtggtttga tctacgattt     3540

ggttatcttc accggtccag aatctccatt aaactaccca gacaacggtt tcatcgacaa     3600

gaaggtccac caaatcactg ccaagttcga aaaggaagaa gaagttgaaa acttggaaaa     3660

gaccgattct ccaattgaaa ataattaaag cgaatttctt atgatttatg atttttatta     3720

ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa     3780

aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat     3840

agcatgaggt cgctcttatt gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa     3900

tcgctcccca tttcacccaa ttgtagatat gctaactcca gcaatgagtt gatgaatctc     3960

ggtgtgtatt ttatgtcctc agaggacaac ctcaaataac cacaaacatc cttcccatat     4020

gctcggtcgt gcttgttgta cctaaaatga agtgaagttc ctatactttc tagagaatag     4080

gaacttctat agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat     4140

actgataaca tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg     4200

atatcaaaaa ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa     4260

caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata     4320

ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt     4380

tataaggtta aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga     4440

caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt     4500

atttgcggat taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg     4560

gccgtcgttt tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg     4620

ccttctgctt agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac     4680

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     4740

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     4800

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     4860

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     4920

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     4980

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     5040

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5100

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5160

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     5220

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag     5280

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     5340

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     5400

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     5460

tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagcttg     5520

cgccgtcccg tcaagtcagc gtaatgctct gcttt                                5555


<210> 33
<211> 4535
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4535
<223> /organism="Artificial Sequence"
      /note="Plasmid containing the expression cassette of the P.
      falciparum glycerol transporter;"
      /mol_type="unassigned DNA"

<400> 33
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggaag cgacttccaa tcgctttgca     1260

tatccagtac cacacccaca ggcgtttgtg cgtgtcgacg ctgcgggtat agaaagggtt     1320

ctttactcta tagtacctcc tcgctcagca tctgcttctt cccaaagatg aacgcggcgt     1380

tatgtcacta acgacgtgca ccaacttgcg gaaagtggaa tcccgttcca aaactggcat     1440

ccactaattg atacatctac acaccgcacg ccttttttct gaagcccact ttcgtggact     1500

ttgccatatg caaaattcat gaagtgtgat accaagtcag catacacctc actagggtag     1560

tttctttggt tgtattgatc atttggttca tcgtggttca ttaatttttt ttctccattg     1620

ctttctggct ttgatcttac tatcatttgg atttttgtcg aaggttgtag aattgtatgt     1680

gacaagtggc accaagcata tataaaaaaa aaaagcatta tcttcctacc agagttgatt     1740

gttaaaaacg tatttatagc aaacgcaatt gtaattaatt cttattttgt atcttttctt     1800

cccttgtctc aatcttttat ttttatttta tttttctttt cttagtttct ttcataacac     1860

caagcaacta atactataac atacaataat aatgcacatg ttgttctaca agtcttacgt     1920

cagagaattc atcggtgaat tcttgggtac tttcgtcttg atgttcttgg gtgaaggtgc     1980

tactgctaac ttccacacca ccggtctatc cggtgactgg tacaagttgt gtttgggttg     2040

gggtttggct gttttcttcg gtatcttggt ttctgccaag ttgtctggtg ctcatttgaa     2100

cttggccgtt tctattggtt tatcttccat caacaaattt gacttgaaga agattccagt     2160

ttacttcttt gctcaattgt tgggtgcttt cgtcggtact tctaccgtct acggtttata     2220

ccacggtttc atctccaact ccaagattcc acaatttgct tgggaaacct ccagaaaccc     2280

atctatctct ttgaccggtg ctttcttcaa cgaattgatt ttgactggta ttttgttgtt     2340

ggtcatcttg gttgttgtcg atgaaaacat ctgtggtaag ttccacattt tgaaattatc     2400

ttccgttgtc ggtttgatca tcttgtgtat cggtatcact ttcggtggta acaccggttt     2460

cgctttgaac ccatctcgtg atttgggttc cagattctta tctttgattg cttacggtaa     2520

ggacactttc accaaggaca acttttactt ctgggttcca ttggttgctc catgtgtcgg     2580

ttccgttgtt ttctgtcaat tctacgacaa ggtcatctgt ccattagtcg atttggccaa     2640

caatgaaaag gatggtgttg acctctaaag cgaatttctt atgatttatg atttttatta     2700

ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa     2760

aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat     2820

agcatgaggt cgctcttatt gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa     2880

tcgctcccca tttcacccaa ttgtagatat gctaactcca gcaatgagtt gatgaatctc     2940

ggtgtgtatt ttatgtcctc agaggacaac ctcaaataac cacaaacatc cttcccatat     3000

gctcggtcgt gcttgttgta cctaaaatga agtgaagttc ctatactttc tagagaatag     3060

gaacttctat agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat     3120

actgataaca tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg     3180

atatcaaaaa ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa     3240

caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata     3300

ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt     3360

tataaggtta aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga     3420

caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt     3480

atttgcggat taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg     3540

gccgtcgttt tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg     3600

ccttctgctt agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac     3660

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     3720

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     3780

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     3840

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     3900

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     3960

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     4020

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     4080

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     4140

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     4200

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag     4260

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     4320

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     4380

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     4440

tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagcttg     4500

cgccgtcccg tcaagtcagc gtaatgctct gcttt                                4535


<210> 34
<211> 4634
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4634
<223> /organism="Artificial Sequence"
      /note="Plasmid containing the expression cassette of the Danio
      rerio glycerol transporter;"
      /mol_type="unassigned DNA"

<400> 34
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggaag cgacttccaa tcgctttgca     1260

tatccagtac cacacccaca ggcgtttgtg cgtgtcgacg ctgcgggtat agaaagggtt     1320

ctttactcta tagtacctcc tcgctcagca tctgcttctt cccaaagatg aacgcggcgt     1380

tatgtcacta acgacgtgca ccaacttgcg gaaagtggaa tcccgttcca aaactggcat     1440

ccactaattg atacatctac acaccgcacg ccttttttct gaagcccact ttcgtggact     1500

ttgccatatg caaaattcat gaagtgtgat accaagtcag catacacctc actagggtag     1560

tttctttggt tgtattgatc atttggttca tcgtggttca ttaatttttt ttctccattg     1620

ctttctggct ttgatcttac tatcatttgg atttttgtcg aaggttgtag aattgtatgt     1680

gacaagtggc accaagcata tataaaaaaa aaaagcatta tcttcctacc agagttgatt     1740

gttaaaaacg tatttatagc aaacgcaatt gtaattaatt cttattttgt atcttttctt     1800

cccttgtctc aatcttttat ttttatttta tttttctttt cttagtttct ttcataacac     1860

caagcaacta atactataac atacaataat aatggaatac ttggaaaaca tcagaaactt     1920

gagaggtcgt tgtgtcttga gaagagatat catcagagaa ttcttggctg aattgttggg     1980

tactttcgtc ttgatcttat tcggttgtgg ttccgttgct caaactgttt tgtccagaga     2040

agctaagggt caattattga ccattcactt cggtttcact ttaggtgtca tgttggctgt     2100

ctacatggct ggtggtgttt ctggtggtca cgttaaccca gctgtctctt tggccatggt     2160

tgttttgaga aagttgccat tgaagaaatt cccagtctac gttttggctc aattcttagg     2220

tgctttcttc ggttcttgtg ccgtctactg tttgtactac gatgctttca ccgaatttgc     2280

taacggtgaa ttggctgtca ccggtccaaa tgtcactgct ggtatctttg cttcttaccc     2340

aagagaaggt ttatctctat tgaacggttt cattgaccaa gtcattggtg ccggtgcttt     2400

ggttttgtgt atcttggccg ttgttgacaa gaagaacatt ggtgctccaa agggtatgga     2460

acctttgttg gttggtctat ccatcttggc catcggtgtt tccatggctt tgaactgtgg     2520

ttacccaatc aacccagctc gtgacttggg tccaagatta ttcaccgcca ttgctggttg     2580

gggtttgacc gtcttttctg ctggtaacgg ttggtggtgg gttccagttg tcggtccaat     2640

ggtcggtggt gttgtcggtg ctgccatcta cttcttgatg attgaaatgc accacccaga     2700

aaacgacaag aacttggaag atgacaactc tttgaaggac aaatacgaat tgaacactgt     2760

taactaaagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa     2820

taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga     2880

gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg     2940

accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat ttcacccaat     3000

tgtagatatg ctaactccag caatgagttg atgaatctcg gtgtgtattt tatgtcctca     3060

gaggacaacc tcaaataacc acaaacatcc ttcccatatg ctcggtcgtg cttgttgtac     3120

ctaaaatgaa gtgaagttcc tatactttct agagaatagg aacttctata gtgagtcgaa     3180

taagggcgac acaaaattta ttctaaatgc ataataaata ctgataacat cttatagttt     3240

gtattatatt ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc     3300

tttattattt tcgagattta ttttcttaat tctctttaac aaactagaaa tattgtatat     3360

acaaaaaatc ataaataata gatgaatagt ttaattatag gtgttcatca atcgaaaaag     3420

caacgtatct tatttaaagt gcgttgcttt tttctcattt ataaggttaa ataattctca     3480

tatatcaagc aaagtgacag gcgcccttaa atattctgac aaatgctctt tccctaaact     3540

ccccccataa aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt aacgattact     3600

cgttatcaga accgcccagg gggcccgagc ttaagactgg ccgtcgtttt acaacacaga     3660

aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc cttctgctta gtttgatgcc     3720

tggcagttcc ctactctcgc cttccgcttc ctcgctcact gactcgctgc gctcggtcgt     3780

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc     3840

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa     3900

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa     3960

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc     4020

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc     4080

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag     4140

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga     4200

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc     4260

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac     4320

agagttcttg aagtggtggg ctaactacgg ctacactaga agaacagtat ttggtatctg     4380

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca     4440

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa     4500

aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc     4560

gcgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg     4620

taatgctctg cttt                                                       4634


<210> 35
<211> 4637
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4637
<223> /organism="Artificial Sequence"
      /note="Plasmid containing the expression cassette of the X.
      tropicalis glycerol transporter;"
      /mol_type="unassigned DNA"

<400> 35
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggaag cgacttccaa tcgctttgca     1260

tatccagtac cacacccaca ggcgtttgtg cgtgtcgacg ctgcgggtat agaaagggtt     1320

ctttactcta tagtacctcc tcgctcagca tctgcttctt cccaaagatg aacgcggcgt     1380

tatgtcacta acgacgtgca ccaacttgcg gaaagtggaa tcccgttcca aaactggcat     1440

ccactaattg atacatctac acaccgcacg ccttttttct gaagcccact ttcgtggact     1500

ttgccatatg caaaattcat gaagtgtgat accaagtcag catacacctc actagggtag     1560

tttctttggt tgtattgatc atttggttca tcgtggttca ttaatttttt ttctccattg     1620

ctttctggct ttgatcttac tatcatttgg atttttgtcg aaggttgtag aattgtatgt     1680

gacaagtggc accaagcata tataaaaaaa aaaagcatta tcttcctacc agagttgatt     1740

gttaaaaacg tatttatagc aaacgcaatt gtaattaatt cttattttgt atcttttctt     1800

cccttgtctc aatcttttat ttttatttta tttttctttt cttagtttct ttcataacac     1860

caagcaacta atactataac atacaataat aatgggtaga caaaaggact tcgtcaacaa     1920

gtgtaaccaa atgttgagat tgagaaacaa gttgttgaga caagctttgt ctgaatgttt     1980

gggtactttg atcttggtta tgttcggttg tggttccgtt gctcaagttg ttttgtccaa     2040

gggttctcac ggtcaattct tgaccgtcaa cttggctttc ggtttcgctg tcatgttggg     2100

tatcttgatt tctggtcaag tttccggtgg tcacttgaac ccagctgtca cctttgctct     2160

atgtatcttg gccagagaac catgggttaa gttcccagtt tactccattg ctcaaacttt     2220

aggtgctttc ttgggtgctg gtatcatcta cggtttatac tacgatgcca tctggtactt     2280

tgctaacgac caattatacg ttaccggtga aaacggtact gccggtatct tcaccacctt     2340

cccatctgac catttgactt tgatgaacgg tttcttcgac caattcatcg gtactgctgc     2400

tttggttgtt tgtgtcttgg ccattgtcga cccaaacaac aacccaatcc caagaggttt     2460

ggaagctttc accgttggtt tcgttgtctt ggtcattggt acttccatgg gtttcaactc     2520

tggttacgct gtcaacccag ctagagattt cggtccaaga ttattcacct ctttggctgg     2580

ttggggtact gaagttttct gggctggtaa ccaatggtgg tgggttccaa ttgtctctcc     2640

attattgggt gcctttgccg gtgtcttagt ctaccaattg atgatcggtt gtcacattga     2700

acctgttcca caatctactg aacaagaaaa catcaaattg gctgatgtca agccaaagga     2760

ccgtatttaa agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa     2820

aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct     2880

tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta     2940

ttgaccacac ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcaccc     3000

aattgtagat atgctaactc cagcaatgag ttgatgaatc tcggtgtgta ttttatgtcc     3060

tcagaggaca acctcaaata accacaaaca tccttcccat atgctcggtc gtgcttgttg     3120

tacctaaaat gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc     3180

gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag     3240

tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt     3300

ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta     3360

tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa     3420

aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc     3480

tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa     3540

actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt     3600

actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac     3660

agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat     3720

gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt     3780

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     3840

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     3900

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     3960

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     4020

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     4080

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct     4140

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     4200

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     4260

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     4320

tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat     4380

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     4440

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     4500

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     4560

cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca     4620

gcgtaatgct ctgcttt                                                    4637


<210> 36
<211> 5537
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5537
<223> /organism="Artificial Sequence"
      /note="Plasmid containing the expression cassette of the Z.
      rouxii glycerol transporter;"
      /mol_type="unassigned DNA"

<400> 36
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggaag cgacttccaa tcgctttgca     1260

tatccagtac cacacccaca ggcgtttgtg cgtgtcgacg ctgcgggtat agaaagggtt     1320

ctttactcta tagtacctcc tcgctcagca tctgcttctt cccaaagatg aacgcggcgt     1380

tatgtcacta acgacgtgca ccaacttgcg gaaagtggaa tcccgttcca aaactggcat     1440

ccactaattg atacatctac acaccgcacg ccttttttct gaagcccact ttcgtggact     1500

ttgccatatg caaaattcat gaagtgtgat accaagtcag catacacctc actagggtag     1560

tttctttggt tgtattgatc atttggttca tcgtggttca ttaatttttt ttctccattg     1620

ctttctggct ttgatcttac tatcatttgg atttttgtcg aaggttgtag aattgtatgt     1680

gacaagtggc accaagcata tataaaaaaa aaaagcatta tcttcctacc agagttgatt     1740

gttaaaaacg tatttatagc aaacgcaatt gtaattaatt cttattttgt atcttttctt     1800

cccttgtctc aatcttttat ttttatttta tttttctttt cttagtttct ttcataacac     1860

caagcaacta atactataac atacaataat aatgggtaag agaactcaag gtttcatgga     1920

ctacgttttc tccagaactt ctactgctgg tttgaaaggt gccagattga gatacactgc     1980

tgctgccgtt gctgttatcg gtttcgcttt attcggttac gaccaaggtt tgatgtctgg     2040

tttgattacc ggtgaccaat tcaacaagga attccctcca accaaatcta acggtgacaa     2100

cgacagatac gcctccgtca tccaaggtgc tgtcactgct tgttacgaaa ttggttgttt     2160

cttcggttct ttgttcgtct tattctttgg tgatgccatc ggtagaaagc cattaatcat     2220

tttcggtgcc atcatcgtca tcattggtac tgttatctcc actgctccat tccaccacgc     2280

ttggggtttg ggtcaatttg ttgtcggtag agttatcact ggtgtcggta ctggtttcaa     2340

cacttccacc atcccagtct ggcaatctga aatgaccaag ccaaacatca gaggtgctat     2400

gattaacttg gatggttccg tcattgcttt cggtactatg attgcttact ggttggattt     2460

cggtttctct ttcatcaact cttctgttca atggagattc ccagtttccg ttcaaatcat     2520

ctttgctttg gtcttattgt tcggtattgt cagaatgcca gaatctccaa gatggttgat     2580

ggccaagaag cgtccagctg aagccagata cgttttggct tgtttgaatg acttgccaga     2640

aaacgacgat gccatcttgg ccgaaatgac ttctttgcac gaagctgtca acagatcttc     2700

taaccaaaag tctcaaatga aatctttgtt ctccatgggt aagcaacaaa acttctccag     2760

agctttgatt gcttcctcta cccaattctt ccaacaattc accggttgta atgctgccat     2820

ctactactcc actgttttgt tccaaaccac cgttcaatta gatagattgt tggctatgat     2880

cttgggtggt gtctttgcta ccgtttacac cttgtccact ttgccatctt tctacttggt     2940

tgaaaaggtt ggtcgtcgta agatgttctt cttcggtgct ttaggtcaag gtatttcttt     3000

cattattacc tttgcttgtt tggtcaaccc aaccaagcaa aacgctaagg gtgctgctgt     3060

cggtttatac ttgttcatca tctgtttcgg tttggctatc ttggaattgc catggatcta     3120

cccaccagaa attgcttcca tgagagtcag agctgctacc aacgctatgt ccacctgtac     3180

caactgggtt accaactttg ccgttgtcat gttcacccca gtctttatcc aaacttctca     3240

atggggttgt tacttgttct tcgctgtcat gaacttcatc tacttgcctg ttatcttctt     3300

cttctaccca gaaactgccg gtcgttcttt ggaagaaatc gatattattt tcgctaaggc     3360

tcatgttgac ggtactctac catggatggt tgctcacaga ttaccaaagt tgtccatgac     3420

tgaagtcgaa gattactctc aatctttggg tttgcacgac gatgaaaacg aaaaggaaga     3480

atacgacgaa aaagaagctg aagctaacgc tgccttgttc caagttgaaa cctcttccaa     3540

gtctccatcc tccaacagaa aggatgacga cgctccaatt gaacacaacg aagttcaaga     3600

atctaacgac aactcctcta actcttctaa cgttgaagct ccaattccag ttcaccacaa     3660

cgacccataa agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa     3720

aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct     3780

tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta     3840

ttgaccacac ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcaccc     3900

aattgtagat atgctaactc cagcaatgag ttgatgaatc tcggtgtgta ttttatgtcc     3960

tcagaggaca acctcaaata accacaaaca tccttcccat atgctcggtc gtgcttgttg     4020

tacctaaaat gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc     4080

gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag     4140

tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt     4200

ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta     4260

tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa     4320

aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc     4380

tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa     4440

actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt     4500

actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac     4560

agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat     4620

gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt     4680

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     4740

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     4800

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     4860

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     4920

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     4980

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct     5040

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     5100

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     5160

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     5220

tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat     5280

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     5340

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     5400

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     5460

cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca     5520

gcgtaatgct ctgcttt                                                    5537


<210> 37
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Forward primer for the amplification of the glycerol
      transporter expression cassettes;"
      /mol_type="unassigned DNA"

<400> 37
aagcgacttc caatcgcttt gc                                             22


<210> 38
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Reverse primer for the amplification of the glycerol
      transporter expression cassettes."
      /mol_type="unassigned DNA"

<400> 38
aggtacaaca agcacgaccg                                                20


<210> 39
<211> 14291
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14291
<223> /organism="Artificial Sequence"
      /note="DNA sequence of plasmid pRN1002"
      /mol_type="unassigned DNA"

<400> 39
gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataagacgtc       60

aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca      120

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa      180

aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt      240

ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca      300

gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag      360

ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc      420

ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca      480

gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt      540

aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct      600

gacaacgatc ggaggaccga aggagctaac cgcttttttt cacaacatgg gggatcatgt      660

aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga      720

caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact      780

tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc      840

acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga      900

gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt      960

agttatctac acgacgggca gtcaggcaac tatggatgaa cgaaatagac agatcgctga     1020

gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact     1080

ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga     1140

taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt     1200

agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca     1260

aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct     1320

ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta     1380

gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct     1440

aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc     1500

aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca     1560

gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga     1620

aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg     1680

aacaggagag cgcacgaggg agcttccagg ggggaacgcc tggtatcttt atagtcctgt     1740

cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggccgag     1800

cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt     1860

tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt     1920

tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga     1980

ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta     2040

atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa     2100

tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcctat     2160

gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta     2220

cgccaagctc ggaattaacc ctcactaaag ggaacaaaag ctgggtaccg ggccccccct     2280

cgaggtcgag cttaagacgc gtttcttctt cagattccct catggagaaa gtgcggcaga     2340

tgtatatgac agagtcgcca gtttccaaga gactttattc aggcacttcc atgataggca     2400

agagagaaga cccagagatg ttgttgtcct agttacacat ggtatttatt ccagagtatt     2460

cctgatgaaa tggtttagat ggacatacga agagtttgaa tcgtttacca atgttcctaa     2520

cgggagcgta atggtgatgg aactggacga atccatcaat agatacgtcc tgaggaccgt     2580

gctacccaaa tggactgatt gtgagggaga cctaactaca tagtgtttaa agattacgga     2640

tatttaactt acttagaata atgccatttt tttgagttat aataatccta cgttagtgtg     2700

agcgggattt aaactgtgag gaccttaata cattcagaca cttctgcggt atcaccctac     2760

ttattccctt cgagattata tctaggaacc catcaggttg gtggaagatt acccgttcta     2820

agacttttca gcttcctcta ttgatgttac acctggacac cccttttctg gcatccagtt     2880

tttaatcttc agtggcatgt gagattctcc gaaattaatt aaagcaatca cacaattctc     2940

tcggatacca cctcggttga aactgacagg tggtttgtta cgcatgctaa tgcaaaggag     3000

cctatatacc tttggctcgg ctgctgtaac agggaatata aagggcagca taatttagga     3060

gtttagtgaa cttgcaacat ttactatttt cccttcttac gtaaatattt ttctttttaa     3120

ttctaaatca atctttttca attttttgtt tgtattcttt tcttgcttaa atctataact     3180

acaaaaaaca catacataaa tctagaaaat gtccgctaaa tcgtttgaag tcacagatcc     3240

agtcaattca agtctcaaag ggtttgccct tgctaacccc tccattacgc tggtccctga     3300

agaaaaaatt ctcttcagaa agaccgattc cgacaagatc gcattaattt ctggtggtgg     3360

tagtggacat gaacctacac acgccggttt cattggtaag ggtatgttga gtggcgccgt     3420

ggttggcgaa atttttgcat ccccttcaac aaaacagatt ttaaatgcaa tccgtttagt     3480

caatgaaaat gcgtctggcg ttttattgat tgtgaagaac tacacaggtg atgttttgca     3540

ttttggtctg tccgctgaga gagcaagagc cttgggtatt aactgccgcg ttgctgtcat     3600

aggtgatgat gttgcagttg gcagagaaaa gggtggtatg gttggtagaa gagcattggc     3660

aggtaccgtt ttggttcata agattgtagg tgccttcgca gaagaatatt ctagtaagta     3720

tggcttagac ggtacagcta aagtggctaa aattatcaac gacaatttgg tgaccattgg     3780

atcttcttta gaccattgta aagttcctgg caggaaattc gaaagtgaat taaacgaaaa     3840

acaaatggaa ttgggtatgg gtattcataa cgaacctggt gtgaaagttt tagaccctat     3900

tccttctacc gaagacttga tctccaagta tatgctacca aaactattgg atccaaacga     3960

taaggataga gcttttgtaa agtttgatga agatgatgaa gttgtcttgt tagttaacaa     4020

tctcggcggt gtttctaatt ttgttattag ttctatcact tccaaaacta cggatttctt     4080

aaaggaaaat tacaacataa ccccggttca aacaattgct ggcacattga tgacctcctt     4140

caatggtaat gggttcagta tcacattact aaacgccact aaggctacaa aggctttgca     4200

atctgatttt gaggagatca aatcagtact agacttgttg aacgcattta cgaacgcacc     4260

gggctggcca attgcagatt ttgaaaagac ttctgcccca tctgttaacg atgacttgtt     4320

acataatgaa gtaacagcaa aggccgtcgg tacctatgac tttgacaagt ttgctgagtg     4380

gatgaagagt ggtgctgaac aagttatcaa gagcgaaccg cacattacgg aactagacaa     4440

tcaagttggt gatggtgatt gtggttacac tttagtggca ggagttaaag gcatcaccga     4500

aaaccttgac aagctgtcga aggactcatt atctcaggcg gttgcccaaa tttcagattt     4560

cattgaaggc tcaatgggag gtacttctgg tggtttatat tctattcttt tgtcgggttt     4620

ttcacacgga ttaattcagg tttgtaaatc aaaggatgaa cccgtcacta aggaaattgt     4680

ggctaagtca ctcggaattg cattggatac tttatacaaa tatacaaagg caaggaaggg     4740

atcatccacc atgattgatg ctttagaacc attcgttaaa gaatttactg catctaagga     4800

tttcaataag gcggtaaaag ctgcagagga aggtgctaaa tccactgcta cattcgaggc     4860

caaatttggc agagcttcgt atgtcggcga ttcatctcaa gtagaagatc ctggtgcagt     4920

aggcctatgt gagtttttga agggggttca aagcgccttg taagtcgaga caaatcgctc     4980

ttaaatatat acctaaagaa cattaaagct atattataag caaagatacg taaattttgc     5040

ttatattatt atacacatat catatttcta tatttttaag atttggttat ataatgtacg     5100

taatgcaaag gaaataaatt ttatacatta ttgaacagcg tccaagtaac tacattatgt     5160

gcactaatag tttagcgtcg tgaagacttt attgtgtcgc gaaaagtaaa aattttaaaa     5220

attagagcac cttgaacttg cgaaaaaggt tctcatcaac tgtttaaaac gtacgcctag     5280

gaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg     5340

tacacgcgtt tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact     5400

aacataacta ttaaaaaaaa taaataggga cctagacttc aggttgtcta actccttcct     5460

tttcggttag agcggatgtg ggaggagggc gtgaatgtaa gcgtgacata actaattaca     5520

tgatatcgac aaaggaaaag gggcgcgcct tattcccatt cttgtaggaa tctttgaccg     5580

tattggtcag caaccaacaa agcagcgtaa acttggtctg gggtagcacc acctggcatg     5640

ttgtggatgg tttcaccttc agcacaagca gcttcagcaa cgattctcat cttagctgga     5700

acgtcttcct tgatgtccaa ttgagccaag gtgattggca aaccaacagc gtgagacaaa     5760

gcagcaacgg tttcgatttc ttcaactgga gcgttttcca aaaccaattg ggtcaaggta     5820

ccgaaagcaa ccttttcacc gtggtagtag tggtgagcgt ctgggatagc ggtcaaaccg     5880

ttgtgaacag cgtgagcagc agccaaacca ccagattcga aaccaacacc agacaagtag     5940

gtgttagctt cgataactct ttccaaagct ggggtaacaa cgtgttgttc agcagccaac     6000

atagcctttt caccttcttc caacaaggtg ttgtaacaca attcagccaa agccaaagca     6060

gcttgggtac acttaccacc agccatggtg gtagcaccag atctagaaca agctctagct     6120

tcgaaccagg tagccaaagc gtcaccgata ccagctgcca acaatctagc tggagcacca     6180

gcaacgatct tggtgtcaac gataaccatg tttgggttgt ttggcaacaa caagtatctg     6240

tcgaattcac cttcgtcggt gtagataaca gacaaagcag aacatggagc gtcggtagaa     6300

gcgatggttg gagcgatagc aactggaaca cccatgaagt gagccaaagc cttagcggtg     6360

tccaaggtct taccaccacc gatacccaag atagcaccac attgagcggt ttcagcgata     6420

cctctcaatc tgtcgatttc gttttgagaa cattcaccac cgaatggagc gatttcaaca     6480

accaaaccag cgtccttgaa agacttttca acggtagatt gagcgaaacc caaaacgaac     6540

ttgtcaccaa caaccaacca tctttcagcc aatggcttca agtattcacc caatctgttg     6600

ataacgtcag caccttggat gtacttacct ggagattgga tgattctgtc cattttctgc     6660

agttaattca gtaaattttc gatcttggga agaaaaaagc agtaagcgtg aaaaatctaa     6720

aagctgatgt agtagaagat cctattcttt aacaaagatt gaccttttct ttttcttctt     6780

ggtttgagta gaaaggggaa ggaagaatac aagagagagg aaaaaaagga agataaaaag     6840

agagcgtgat ataaatgaat atatattaaa caagagagat tgggaaggaa aggatcaaac     6900

aaacccaaaa atatttcaaa aaggagagag agaggcgagt ttggtttcaa aacggtttat     6960

ttatttatgc aagaggacgt ggaagaaaaa gaagaaggaa gaaaaaaatt tgaaagaaaa     7020

aaacgcgtgg cgggtaaaga agaaaatgga aaatagaggc cgggtgacag agaaatattg     7080

agggttaatt ggaaaatatg ttagggtgag gcatatgttt ttaagggttt tgaggatccg     7140

ataaggaaga atgtaggtta aatgttgtgc attaattgct gtggcagctt acccgcttcc     7200

ccacacattt actagtctcg agctcttcaa ctcaagacgc acagatatta taacatctgc     7260

ataataggca tttgcaagaa ttactcgtga gtaaggaaag agtgaggaac tatcgcatac     7320

ctgcatttaa agatgccgat ttgggcgcga atcctttatt ttggcttcac cctcatacta     7380

ttatcagggc cagaaaaagg aagtgtttcc ctccttcttg aattgatgtt accctcataa     7440

agcacgtggc ctcttatcga gaaagaaatt accgtcgctc gtgatttgtt tgcaaaaaga     7500

acaaaactga aaaaacccag acacgctcga cttcctgtct tcctattgat tgcagcttcc     7560

aatttcgtca cacaacaagg tcctagcgac ggctcacagg ttttgtaaca agcaatcgaa     7620

ggttctggaa tggcgggaaa gggtttagta ccacatgcta tgatgcccac tgtgatctcc     7680

agagcaaagt tcgttcgatc gtactgttac tctctctctt tcaaacagaa ttgtccgaat     7740

cgtgtgacaa caacagcctg ttctcacaca ctcttttctt ctaaccaagg gggtggttta     7800

gtttagtaga acctcgtgaa acttacattt acatatatat aaacttgcat aaattggtca     7860

atgcaagaaa tacatatttg gtcttttcta attcgtagtt tttcaagttc ttagatgctt     7920

tctttttctc ttttttacag atcatcaagg aagtaattat ctacttttta caacaaatat     7980

atctagaaaa tggctgttac caacgttgct gaattgaacg ctttggttga aagggttaag     8040

aaggctcaaa gagaatacgc ttctttcacc caagaacaag ttgacaagat cttcagagct     8100

gctgctttgg ctgctgctga cgctagaatc ccattggcta agatggctgt tgctgaatct     8160

ggtatgggta tcgttgaaga caaggttatc aagaaccact tcgcttctga atacatctac     8220

aacgcttaca aggacgaaaa gacctgtggt gttttgtcag aagacgacac cttcggtacc     8280

atcaccatcg ctgaaccaat cggtatcatc tgtggtatcg ttccaaccac caacccaacc     8340

tctaccgcta tcttcaagtc tttgatctct ttgaagacca gaaacgctat catcttctct     8400

ccacacccaa gagctaaaga cgctaccaac aaggctgctg acatcgtttt gcaagctgct     8460

atcgctgctg gtgctccaaa ggacttgatc ggttggatcg accaaccatc tgttgaattg     8520

tctaacgctt tgatgcacca cccagacatc aacttgatct tggctaccgg tggtccaggt     8580

atggttaagg ctgcttactc ttctggtaag ccagctatcg gtgttggtgc tggtaacacc     8640

ccagttgtta tcgacgaaac cgctgacatc aagagagctg ttgcttctgt tttgatgtct     8700

aagaccttcg acaacggtgt tatctgtgct tctgaacaat ctgttgttgt tgttgactct     8760

gtttacgacg ctgttagaga aagattcgct acccacggtg gttacttgtt gcaaggtaag     8820

gaattgaagg ctgttcaaga cgttatcttg aagaacggtg ctttgaacgc tgctatcgtt     8880

ggtcaaccag cttacaagat cgctgaatta gctggtttct ctgttccaga aaacaccaag     8940

atcttgatcg gtgaagttac cgttgttgac gaatctgaac cattcgctca cgaaaagttg     9000

tctccaacct tggctatgta cagagctaag gacttcgaag acgctgttga aaaagctgaa     9060

aagttggttg ctatgggtgg tattggtcac acctcttgtt tgtacaccga ccaagacaac     9120

caaccagcta gagtttctta cttcggtcaa aagatgaaga ccgctagaat cttgatcaac     9180

accccagctt ctcaaggtgg tatcggtgac ttgtacaact tcaagttggc tccatctttg     9240

accttgggtt gtggttcttg gggtggtaac tctatctctg aaaacgttgg tccaaagcac     9300

ttgatcaaca agaagaccgt tgctaagaga gctgaaaaca tgttgtggca caagttgcca     9360

aaatctatct acttcagaag aggttctttg ccaatcgctt tggacgaagt tatcaccgac     9420

ggtcacaaga gagctttgat cgttaccgac agattcttgt tcaacaacgg ttacgctgac     9480

caaatcacct ctgttttgaa ggctgctggt gttgaaaccg aagttttctt cgaagttgaa     9540

gctgacccaa ccttgtctat cgttagaaag ggtgctgaat tggctaactc tttcaagcca     9600

gacgttatca tcgctttggg tggtggttct ccaatggacg ctgctaagat catgtgggtt     9660

atgtacgaac acccagaaac ccacttcgaa gaattggctt tgagattcat ggacatcaga     9720

aagagaatct acaagttccc aaagatgggt gttaaggcta agatgatcgc tgttaccacc     9780

acctctggta ccggttctga agttacccca ttcgctgttg ttaccgacga cgctaccggt     9840

caaaagtacc cattggctga ctacgctttg accccagaca tggctatcgt tgacgctaac     9900

ttggttatgg acatgccaaa gtctttgtgt gctttcggtg gtttggacgc tgttacccac     9960

gctatggaag cttacgtttc tgttttggct tctgaattct ctgacggtca agctttgcaa    10020

gctttgaagt tgttgaagga atacttgcca gcttcttacc acgaaggttc taagaaccca    10080

gttgctagag aaagagttca ctctgctgct accatcgctg gtatcgcttt cgctaacgct    10140

ttcttgggtg tttgtcactc tatggctcac aagttgggtt ctcaattcca catcccacac    10200

ggtttggcta acgctttgtt gatctgtaac gttatcagat acaacgctaa cgacaaccca    10260

accaagcaaa ccgctttctc tcaatacgac agaccacaag ctagaagaag atacgctgaa    10320

atcgctgacc acttgggttt gtctgctcca ggtgacagaa ccgctgcaaa gatcgaaaag    10380

ttgttggctt ggttggaaac cttgaaggct gaattgggta tcccaaagtc tatcagagaa    10440

gctggtgttc aagaagctga cttcttggct aacgttgaca agttgtctga agacgctttc    10500

gacgaccaat gtaccggtgc taacccaaga tacccattga tctctgaatt gaagcaaatc    10560

ttgttggaca cctactacgg tagagactac gttgaaggtg aaaccgctgc taagaaggaa    10620

gctgctccag ctaaggctga aaagaaggct aagaagtctg cttagcttaa gcgcgcgaat    10680

ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa    10740

ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg    10800

taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc    10860

ggcatgccga gcaaatgcct gcaaatcgct ccccatttca cccaattgta gatatgctaa    10920

ctccagcaat gagttgatga atctcggtgt gtattttatg tcctcagagg acaacacctg    10980

ttgtaatcgt tcttccacac gtacgaagct taaaagggcg aattctgcag atatccatca    11040

cactggcggc cgcatgctag ctccggatta tcgatgataa gctgtcaaag atgagaatta    11100

attccacgga ctatagacta tactagatac tccgtctact gtacgataca cttccgctca    11160

ggtccttgtc ctttaacgag gccttaccac tcttttgtta ctctattgat ccagctcagc    11220

aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt aaaactagct agaccgagaa    11280

agagactaga aatgcaaaag gcacttctac aatggctgcc atcattatta tccgatgtga    11340

cgctgcagct tctcaatgat attcgaatac gctttgagga gatacagcct aatatccgac    11400

aaactgtttt acagatttac gatcgtactt gttacccatc attgaatttt gaacatccga    11460

acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa tatatagtct    11520

agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt gcatctattg    11580

cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat cttgcacttc    11640

aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg    11700

cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca    11760

acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg    11820

caacgcgacg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca    11880

gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta    11940

caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct    12000

cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg    12060

ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt    12120

cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc    12180

cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt    12240

gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact    12300

acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta    12360

ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga    12420

gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac    12480

agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatgc    12540

cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt    12600

acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc    12660

ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt    12720

gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat    12780

tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa    12840

ttttaacaaa atattaacgt ttacaatttc ctgatgcggt attttctcct tacgcatctg    12900

tgcggtattt cacaccgcat atgatccgtc gagttcaaga gaaaaaaaaa gaaaaagcaa    12960

aaagaaaaaa ggaaagcgcg cctcgttcag aatgacacgt atagaatgat gcattacctt    13020

gtcatcttca gtatcatact gttcgtatac atacttactg acattcatag gtatacatat    13080

atacacatgt atatatatcg tatgctgcag ctttaaataa tcggtgtcac tacataagaa    13140

cacctttggt ggagggaaca tcgttggtac cattgggcga ggtggcttct cttatggcaa    13200

ccgcaagagc cttgaacgca ctctcactac ggtgatgatc attcttgcct cgcagacaat    13260

caacgtggag ggtaattctg ctagcctctg caaagctttc aagaaaatgc gggatcatct    13320

cgcaagagag atctcctact ttctcccttt gcaaaccaag ttcgacaact gcgtacggcc    13380

tgttcgaaag atctaccacc gctctggaaa gtgcctcatc caaaggcgca aatcctgatc    13440

caaacctttt tactccacgc gccagtaggg cctctttaaa agcttgaccg agagcaatcc    13500

cgcagtcttc agtggtgtga tggtcgtcta tgtgtaagtc accaatgcac tcaacgatta    13560

gcgaccagcc ggaatgcttg gccagagcat gtatcatatg gtccagaaac cctatacctg    13620

tgtggacgtt aatcacttgc gattgtgtgg cctgttctgc tactgcttct gcctcttttt    13680

ctgggaagat cgagtgctct atcgctaggg gaccaccctt taaagagatc gcaatctgaa    13740

tcttggtttc atttgtaata cgctttacta gggctttctg ctctgtcatc tttgccttcg    13800

tttatcttgc ctgctcattt tttagtatat tcttcgaaga aatcacatta ctttatataa    13860

tgtataattc attatgtgat aatgccaatc gctaagaaaa aaaaagagtc atccgctagg    13920

tggaaaaaaa aaaatgaaaa tcattaccga ggcataaaaa aatatagagt gtactagagg    13980

aggccaagag taatagaaaa agaaaattgc gggaaaggac tgtgttatga cttccctgac    14040

taatgccgtg ttcaaacgat acctggcagt gactcctagc gctcaccaag ctcttaaaac    14100

ggaattatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg    14160

acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta    14220

cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc    14280

gaaacgcgcg a                                                         14291


<210> 40
<211> 2407
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2407
<223> /organism="Artificial Sequence"
      /note="DNA fragment of plasmid pRN995, containing the
      INO4-promotor, the GUP1 ORF and the CYC1-terminator"
      /mol_type="unassigned DNA"

<400> 40
tagaactaat gaggtggggt acctccaaat ctgcgaaggt accatttgca aaagcggcga      60

agtaatcaag ttgtagtcca agttagggtc ttcaagaact agcttgttgt acaaggatct     120

ccttatacag aaaagggttg cagttaagca tatataagtg actgtaataa atttagtagc     180

atcaggaatc ttattgatat ttaaaaatga atccgggata ttcaattcta ggaacctcga     240

actatattgc attatagtga acaaatatct ttgttataaa tagattagtc gtcttctttt     300

tcttggcttg gtttgtatga gcttgttacg tatcaaacaa aatattcaca tgtttttctc     360

accttaaaga cagaggcgcg attgcgtttg cgaaattcca atgccaacaa gataagcttg     420

acgaaggagt taagagggcg gcttgaacta aaaagagaaa agaattcgaa aatgtcgctg     480

atcagcatcc tgtctcccct aattacttcc gagggcttag attcaagaat caaaccttca     540

ccaaaaaagg atgcctctac taccactaag ccatcactat ggaaaactac tgagttcaaa     600

ttctactaca ttgcatttct ggtcgtggtt cccttgatgt tctatgctgg gttacaagct     660

agttcgcccg aaaatccaaa ctatgcaaga tacgaacgtc tcctatctca aggttggtta     720

tttggcagaa aagtagacaa tagtgattct caatataggt ttttcaggga caattttgcg     780

ctattgtcag ttttaatgct agtccacact tctataaaac gcattgtact ttattcaaca     840

aatatcacta aattgaggtt tgatctgata tttggtttga tctttttagt ggccgctcat     900

ggtgtcaatt cgataagaat tttagcccat atgctaattt tatatgccat cgcccatgta     960

ctaaagaact ttagaagaat agccaccatc agcatttgga tttatggtat ttctacgctt    1020

tttattaacg acaacttcag agcatatcca tttggtaata tttgctcttt tttaagccca    1080

ttggaccatt ggtatagagg tatcattcca agatgggatg tctttttcaa ttttactctt    1140

ttgagagtct taagttacaa cttggacttc ttagagaggt gggagaattt acaaaagaag    1200

aaaagtccat cctatgaatc aaaagaagct aaatcagcca ttttgctcaa tgaacgtgct    1260

agattaactg ctgcacaccc catacaggac tacagcttaa tgaattatat tgcatatgtt    1320

acttacacgc cacttttcat tgccggcccc attataacat tcaatgatta tgtttaccaa    1380

tcgaaacata ccttgccatc aataaatttc aaattcattt tttactatgc ggtgagattc    1440

gttattgctc tcttatctat ggagttcatt ttacactttc tccacgttgt ggcaatctca    1500

aaaaccaaag cgtgggaaaa tgacacacct ttccagattt ccatgattgg cttatttaat    1560

ttgaatatta tttggctaaa actactgatt ccgtggaggc tgtttaggct gtgggctttg    1620

ctagacggaa tcgatacacc tgaaaatatg atcaggtgtg ttgataacaa ttacagttca    1680

ctagcattct ggagagcttg gcatagaagc tacaataagt gggttgtccg ttacatatat    1740

attcctctag gtggttcaaa aaatagagtt ttgacatcac tagcagtctt ttccttcgta    1800

gctatatggc atgacatcga actaaagtta ttattatggg gttggctaat agttttgttc    1860

ctcttaccag aaatttttgc tacccaaatt ttctctcatt ataccgacgc agtctggtac    1920

agacacgttt gcgctgtcgg tgctgttttc aacatatggg ttatgatgat cgctaatctt    1980

tttggattct gcttgggctc tgacggtact aaaaaattac taagcgatat gttctgtacc    2040

gtatctggtt tcaaatttgt aattttggca agcgttagtt tattcatcgc agtacaaata    2100

atgtttgaaa tcagagaaga agaaaagagg cacggaattt acctaaaatg ctgaggatcc    2160

ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcctc    2220

ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt    2280

atttttttta atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt    2340

ttttctgtac aaacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt    2400

tttggga                                                              2407


<210> 41
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..85
<223> /organism="Artificial Sequence"
      /note="synthetic DNA, forward primer for amplification of
      loxP-kanMX-loxP with GUP1 flanking sequence"
      /mol_type="unassigned DNA"

<400> 41
acaaaggcaa aaacaaagcg tgaatcagca tttgttaaca gacaattgca agaaatgtcg     60

ggcctataac ttcgtatagc ataca                                           85


<210> 42
<211> 86
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..86
<223> /organism="Artificial Sequence"
      /note="synthetic DNA, reverse primer for amplification of
      loxP-kanMX-loxP with GUP1 flanking sequence"
      /mol_type="unassigned DNA"

<400> 42
taaaaataca tacatgatag cagtgttata caattgatat tcgtaaattt ggcattcagc     60

ctagcataac ttcgtataat gtatgc                                          86


<210> 43
<211> 5088
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5088
<223> /organism="Artificial Sequence"
      /note="sequence of the plasmid pRN887"
      /mol_type="unassigned DNA"

<400> 43
tcatgacgtc tcgaggccta taacttcgta tagcatacat tatacgaagt tatgcgcgct       60

ctagatatcg tcgacactgg atggcggcgt tagtatcgaa tcgacagcag tatagcgacc      120

agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt cgcatctggg      180

cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta aaatagaaca      240

actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat ctttttattg      300

tcagtactga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag      360

gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga      420

ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat      480

caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat      540

gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt      600

caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca      660

ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa      720

caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg      780

aatcaggata ttcttctaat acctggaatg ctgttttgcc ggggatcgca gtggtgagta      840

accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg      900

tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat      960

gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg     1020

attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat     1080

ttaatcgcgg cctcgaaacg tgagtctttt ccttacccat ggttgtttat gttcggatgt     1140

gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga agaacctcag     1200

tggcaaatcc taacctttta tatttctcta caggggcgcg gcgtggggac aattcaacgc     1260

gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt aatttttgct     1320

tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg gatgtatggg     1380

ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa gactgtcaag     1440

gagggtattc tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg aggcaagctt     1500

aagggcgaat tccagcacac tggcggccgt tactagaata acttcgtata gcatacatta     1560

tacgaagtta tgctagcgct ccggattaag ggcgaattct gagatatcca tcacactggc     1620

ggccgctcga gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt     1680

cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc     1740

gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc     1800

gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac acctataaaa     1860

gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgccggggc     1920

gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac     1980

tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca     2040

gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca     2100

tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg agattatcaa     2160

aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa cggtgctgac     2220

cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa     2280

agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag     2340

caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag     2400

taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca agctctgatc     2460

aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc     2520

cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct     2580

ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg     2640

acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca     2700

cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc     2760

tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga     2820

aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc     2880

cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc     2940

ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg     3000

ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct     3060

gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc     3120

tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc     3180

ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc     3240

agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattattaac gcttacaatt     3300

tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacaggtgg     3360

cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa     3420

tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatagc acgtgaggag     3480

ggccaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg     3540

agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt     3600

cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt     3660

gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg     3720

gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg     3780

cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt     3840

cgtggccgag gagcaggact gacacgtgct aaaacttcat ttttaattta aaaggatcta     3900

ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca     3960

ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg     4020

cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga     4080

tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa     4140

tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc     4200

tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg     4260

tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac     4320

ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct     4380

acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc     4440

ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg     4500

gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg     4560

ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct     4620

gggcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga     4680

taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg     4740

cagcgagtca agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     4800

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat     4860

gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg     4920

ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac     4980

gccaagctat ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag     5040

ctcggatcca ctagtaacgg ccgccagtgt gctggaattc gcccttaa                  5088


