                         SEQUENCE LISTING

<110>  Universitaet zu Koeln
 
<120>  Anti CD30 chimeric antigen receptor and its use

<130>  4677-003

<150>  EP13181668.8
<151>  2013-08-26

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  756
<212>  DNA
<213>  human


<220>
<221>  CDS
<222>  (1)..(756)

<400>  1
aga atg gcc cag gtg caa ctg cag cag tca ggg gct gag ctg gct aga         48
Arg Met Ala Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg           
1               5                   10                  15                

cct ggg gct tca gtg aag atg tcc tgc aag gct tct ggc tac acc ttt         96
Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe           
            20                  25                  30                    

act acc tac aca ata cac tgg gta aga cgg agg cct gga cac gat ctg        144
Thr Thr Tyr Thr Ile His Trp Val Arg Arg Arg Pro Gly His Asp Leu           
        35                  40                  45                        

gaa tgg att gga tac att aat cct agc agt gga tgt tct gac tac aat        192
Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Cys Ser Asp Tyr Asn           
    50                  55                  60                            

caa aac ttc aag ggc aag acc aca ttg act gca gac aag tcc tcc aac        240
Gln Asn Phe Lys Gly Lys Thr Thr Leu Thr Ala Asp Lys Ser Ser Asn           
65                  70                  75                  80            

aca gcc tac atg caa ctg aac agc ctg aca tct gag gac tct gcg gtc        288
Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val           
                85                  90                  95                

tat tac tgt gca aga aga gcg gac tat ggt aac tac gaa tat acc tgg        336
Tyr Tyr Cys Ala Arg Arg Ala Asp Tyr Gly Asn Tyr Glu Tyr Thr Trp           
            100                 105                 110                   

ttt gct tac tgg ggc caa ggg acc acg gtc acc gtc tcc tca agt gga        384
Phe Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ser Gly           
        115                 120                 125                       

ggc ggt tca ggt gga ggt ggc tct ggc ggt ggc gga tcg gtc atc gag        432
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Ile Glu           
    130                 135                 140                           

ctc act cag tct cca aaa ttc atg tcc aca tca gta gga gac agg gtc        480
Leu Thr Gln Ser Pro Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val           
145                 150                 155                 160           

aac gtc acc tac aag gcc agt cag aat gtg ggt act aat gta gcc tgg        528
Asn Val Thr Tyr Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp           
                165                 170                 175               

ttt caa caa aaa cca ggg caa tct cct aaa gtt ctg att tac tcg gca        576
Phe Gln Gln Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Ser Ala           
            180                 185                 190                   

tct tac cga tac agt gga gtc cct gat cgc ttc aca ggc agt gga tct        624
Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser           
        195                 200                 205                       

gga aca gat ttc act ctc acc atc agc aat gtg cag tct gaa gac ttg        672
Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser Glu Asp Leu           
    210                 215                 220                           

gca gag tat ttc tgt cag caa tat cac acc tat cct ctc acg ttc gga        720
Ala Glu Tyr Phe Cys Gln Gln Tyr His Thr Tyr Pro Leu Thr Phe Gly           
225                 230                 235                 240           

ggg ggc acc aag ctg gaa atc aaa cgg tcg gat ccc                        756
Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Asp Pro                           
                245                 250                                   


<210>  2
<211>  252
<212>  PRT
<213>  human

<400>  2

Arg Met Ala Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
1               5                   10                  15      


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
            20                  25                  30          


Thr Thr Tyr Thr Ile His Trp Val Arg Arg Arg Pro Gly His Asp Leu 
        35                  40                  45              


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Cys Ser Asp Tyr Asn 
    50                  55                  60                  


Gln Asn Phe Lys Gly Lys Thr Thr Leu Thr Ala Asp Lys Ser Ser Asn 
65                  70                  75                  80  


Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Arg Ala Asp Tyr Gly Asn Tyr Glu Tyr Thr Trp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ser Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Ile Glu 
    130                 135                 140                 


Leu Thr Gln Ser Pro Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val 
145                 150                 155                 160 


Asn Val Thr Tyr Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp 
                165                 170                 175     


Phe Gln Gln Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Ser Ala 
            180                 185                 190         


Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser Glu Asp Leu 
    210                 215                 220                 


Ala Glu Tyr Phe Cys Gln Gln Tyr His Thr Tyr Pro Leu Thr Phe Gly 
225                 230                 235                 240 


Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Asp Pro 
                245                 250         


<210>  3
<211>  690
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion polypeptide

<400>  3

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 
1               5                   10                  15      


Val Ile Met Ser Arg Met Ala Gln Val Gln Leu Gln Gln Ser Gly Ala 
            20                  25                  30          


Glu Leu Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser 
        35                  40                  45              


Gly Tyr Thr Phe Thr Thr Tyr Thr Ile His Trp Val Arg Arg Arg Pro 
    50                  55                  60                  


Gly His Asp Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Cys 
65                  70                  75                  80  


Ser Asp Tyr Asn Gln Asn Phe Lys Gly Lys Thr Thr Leu Thr Ala Asp 
                85                  90                  95      


Lys Ser Ser Asn Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu 
            100                 105                 110         


Asp Ser Ala Val Tyr Tyr Cys Ala Arg Arg Ala Asp Tyr Gly Asn Tyr 
        115                 120                 125             


Glu Tyr Thr Trp Phe Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val 
    130                 135                 140                 


Ser Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Val Ile Glu Leu Thr Gln Ser Pro Lys Phe Met Ser Thr Ser Val 
                165                 170                 175     


Gly Asp Arg Val Asn Val Thr Tyr Lys Ala Ser Gln Asn Val Gly Thr 
            180                 185                 190         


Asn Val Ala Trp Phe Gln Gln Lys Pro Gly Gln Ser Pro Lys Val Leu 
        195                 200                 205             


Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln 
225                 230                 235                 240 


Ser Glu Asp Leu Ala Glu Tyr Phe Cys Gln Gln Tyr His Thr Tyr Pro 
                245                 250                 255     


Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Asp Pro 
            260                 265                 270         


Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        275                 280                 285             


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    290                 295                 300                 


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
305                 310                 315                 320 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                325                 330                 335     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            340                 345                 350         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        355                 360                 365             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    370                 375                 380                 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
385                 390                 395                 400 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
                405                 410                 415     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            420                 425                 430         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        435                 440                 445             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    450                 455                 460                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
465                 470                 475                 480 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                485                 490                 495     


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe Trp Val Leu 
            500                 505                 510         


Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val 
        515                 520                 525             


Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His 
    530                 535                 540                 


Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys 
545                 550                 555                 560 


His Tyr Gln Ala Tyr Ala Ala Ala Arg Asp Phe Ala Ala Tyr Arg Ser 
                565                 570                 575     


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
            580                 585                 590         


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
        595                 600                 605             


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
    610                 615                 620                 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
625                 630                 635                 640 


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
                645                 650                 655     


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
            660                 665                 670         


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
        675                 680                 685             


Arg Glx 
    690 


<210>  4
<211>  2070
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid encoding fusion peptide CAR 1138

<400>  4
atggattttc aggtgcagat tttcagcttc ctgctaatca gtgcctcagt cataatgtct       60

agaatggccc aggtgcaact gcagcagtca ggggctgagc tggctagacc tggggcttca      120

gtgaagatgt cctgcaaggc ttctggctac acctttacta cctacacaat acactgggta      180

agacggaggc ctggacacga tctggaatgg attggataca ttaatcctag cagtggatgt      240

tctgactaca atcaaaactt caagggcaag accacattga ctgcagacaa gtcctccaac      300

acagcctaca tgcaactgaa cagcctgaca tctgaggact ctgcggtcta ttactgtgca      360

agaagagcgg actatggtaa ctacgaatat acctggtttg cttactgggg ccaagggacc      420

acggtcaccg tctcctcaag tggaggcggt tcaggtggag gtggctctgg cggtggcgga      480

tcggtcatcg agctcactca gtctccaaaa ttcatgtcca catcagtagg agacagggtc      540

aacgtcacct acaaggccag tcagaatgtg ggtactaatg tagcctggtt tcaacaaaaa      600

ccagggcaat ctcctaaagt tctgatttac tcggcatctt accgatacag tggagtccct      660

gatcgcttca caggcagtgg atctggaaca gatttcactc tcaccatcag caatgtgcag      720

tctgaagact tggcagagta tttctgtcag caatatcaca cctatcctct cacgttcgga      780

gggggcacca agctggaaat caaacggtcg gatcccgccg agcccaaatc tcctgacaaa      840

actcacacat gcccaccgtg cccagcacct ccagtcgcgg gaccgtcagt cttcctcttc      900

cccccaaaac ccaaggacac cctcatgatc gcccggaccc ctgaggtcac atgcgtggtg      960

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag     1020

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc     1080

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc     1140

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc     1200

cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc     1260

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     1320

aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     1380

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     1440

tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg     1500

tctccgggta aaaaagatcc caaattttgg gtgctggtgg tggttggtgg agtcctggct     1560

tgctatagct tgctagtaac agtggccttt attattttct gggtgaggag taagaggagc     1620

aggctcctgc acagtgacta catgaacatg actccccgcc gccccgggcc cacccgcaag     1680

cattaccagg cctatgccgc cgcacgcgac ttcgcagcct atcgctccct gagagtgaag     1740

ttcagcagga gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataccgag     1800

ctcaatctag gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct     1860

gagatggggg gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgacctgcag     1920

aaagataaga tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc     1980

aaggggcacg atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc     2040

cttcacatgc aggccctgcc ccctcgctaa                                      2070


<210>  5
<211>  693
<212>  DNA
<213>  artificial

<220>
<223>  synthetic nucleic acid sequence

<400>  5
gagcccaaat ctcctgacaa aactcacaca tgcccaccgt gcccagcacc tccagtcgcg       60

ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat cgcccggacc      120

cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac      180

tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac      240

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc      300

aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc      360

tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggat      420

gagctgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta tcccagcgac      480

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc      540

gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga caagagcagg      600

tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac      660

acgcagaaga gcctctccct gtctccgggt aaa                                   693


<210>  6
<211>  204
<212>  DNA
<213>  artificial

<220>
<223>  synthetic sequence

<400>  6
ttttgggtgc tggtggtggt tggtggagtc ctggcttgct atagcttgct agtaacagtg       60

gcctttatta ttttctgggt gaggagtaag aggagcaggc tcctgcacag tgactacatg      120

aacatgactc cccgccgccc cgggcccacc cgcaagcatt accaggccta tgccgccgca      180

cgcgacttcg cagcctatcg ctcc                                             204


<210>  7
<211>  342
<212>  DNA
<213>  artificial

<220>
<223>  synthetic sequence

<400>  7
ctgagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag       60

ctctataccg agctcaatct aggacgaaga gaggagtacg atgttttgga caagagacgt      120

ggccgggacc ctgagatggg gggaaagccg agaaggaaga accctcagga aggcctgtac      180

aatgacctgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag      240

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac      300

acctacgacg cccttcacat gcaggccctg ccccctcgct aa                         342


<210>  8
<211>  198
<212>  DNA
<213>  artificial

<220>
<223>  synthetic sequence

<400>  8
cctcagctct gctatatcct ggatgccatc ctgtttctgt atggaattgt cctcaccctc       60

ctctactgtc gactgaagat ccaagtgcga aaggcagcta taaccagcta tgagaaatca      120

gatggtgttt acacgggcct gagcaccagg aaccaggaga cttacgagac tctgaagcat      180

gagaaaccac cacagtag                                                    198


<210>  9
<211>  25
<212>  PRT
<213>  artificial

<220>
<223>  synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (8)..(20)
<223>  SVFLFPPKPKDTL

<400>  9

Pro Glu Leu Leu Gly Gly Pro Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Met Ile Ser Arg Thr 
            20                  25  


<210>  10
<211>  24
<212>  PRT
<213>  artificial

<220>
<223>  synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(19)
<223>  SVFLFPPKPKDTL

<400>  10

Pro Pro Val Ala Gly Pro Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Met Ile Ala Arg Thr 
            20                  


