                         SEQUENCE LISTING

<110>  Evonik Industries AG
 
<120>  A method for producing acyl amino acids

<130>  201300087WO

<150>  13181841.1
<151>  2013-08-27

<150>  14169650.0
<151>  2014-05-23

<160>  57    

<170>  PatentIn version 3.5

<210>  1
<211>  906
<212>  DNA
<213>  Umbellularia californica


<220>
<221>  misc_feature
<222>  (1)..(906)
<223>  synUcTE (an acyl-CoA thioesterase) gene (codon-optimized)

<400>  1
atgactctag agtggaaacc gaaaccaaaa ctgcctcaac tgctggatga tcacttcggt       60

ctgcacggtc tggtgtttcg tcgtactttc gcaattcgtt cttatgaagt gggtccagat      120

cgttctacct ccatcctggc cgtcatgaac cacatgcagg aagccaccct gaatcacgcg      180

aaatctgttg gtatcctggg tgatggtttc ggcactactc tggaaatgtc taaacgtgac      240

ctgatgtggg tagtgcgtcg cacccacgta gcagtagagc gctaccctac ttggggtgac      300

actgtggaag tcgagtgttg gattggcgcg tccggtaaca atggtatgcg tcgcgatttt      360

ctggtccgtg actgtaaaac gggcgaaatc ctgacgcgtt gcacctccct gagcgttctg      420

atgaacaccc gcactcgtcg cctgtctacc atcccggacg aagtgcgcgg tgagatcggt      480

cctgctttca tcgataacgt ggcagttaaa gacgacgaaa tcaagaaact gcaaaaactg      540

aacgactcca ccgcggacta catccagggc ggtctgactc cgcgctggaa cgacctggat      600

gttaatcagc atgtgaacaa cctgaaatac gttgcttggg tcttcgagac tgtgccggac      660

agcattttcg aaagccatca catttcctct tttactctgg agtaccgtcg cgaatgtact      720

cgcgactccg ttctgcgcag cctgaccacc gtaagcggcg gttctagcga ggcaggtctg      780

gtctgcgacc atctgctgca actggaaggc ggctccgaag tcctgcgtgc gcgtacggag      840

tggcgtccaa agctgacgga ttctttccgc ggcatctccg taattccggc ggaacctcgt      900

gtttaa                                                                 906


<210>  2
<211>  159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tac promotor for synUcTE expression

<400>  2
ggatccaatt gtgagcggat aacaattacg agcttcatgc acagtgatcg acgctgttga       60

caattaatca tcggctcgta taatgtgtgg atgtggaatt gtgagcgctc acaattccac      120

aacggtttcc ctctagaaat aattttgttt aacaggagg                             159


<210>  3
<211>  4977
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pJ294[Ptac-synUcTE]

<400>  3
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag       60

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca      120

gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc      180

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt      240

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg      300

ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca      360

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg      420

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca      480

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg      540

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct      600

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca      660

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca      720

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg      780

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac      840

ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt      900

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca      960

gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg     1020

gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg     1080

ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga     1140

gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg     1200

cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta     1260

gaaagtatag gaacttctga agtgggggcg gccgcaaatt cagtaagcag aaagtcaaaa     1320

gcctccgacc ggaggctttt gactaaagct taaacacgag gttccgccgg aattacggag     1380

atgccgcgga aagaatccgt cagctttgga cgccactccg tacgcgcacg caggacttcg     1440

gagccgcctt ccagttgcag cagatggtcg cagaccagac ctgcctcgct agaaccgccg     1500

cttacggtgg tcaggctgcg cagaacggag tcgcgagtac attcgcgacg gtactccaga     1560

gtaaaagagg aaatgtgatg gctttcgaaa atgctgtccg gcacagtctc gaagacccaa     1620

gcaacgtatt tcaggttgtt cacatgctga ttaacatcca ggtcgttcca gcgcggagtc     1680

agaccgccct ggatgtagtc cgcggtggag tcgttcagtt tttgcagttt cttgatttcg     1740

tcgtctttaa ctgccacgtt atcgatgaaa gcaggaccga tctcaccgcg cacttcgtcc     1800

gggatggtag acaggcgacg agtgcgggtg ttcatcagaa cgctcaggga ggtgcaacgc     1860

gtcaggattt cgcccgtttt acagtcacgg accagaaaat cgcgacgcat accattgtta     1920

ccggacgcgc caatccaaca ctcgacttcc acagtgtcac cccaagtagg gtagcgctct     1980

actgctacgt gggtgcgacg cactacccac atcaggtcac gtttagacat ttccagagta     2040

gtgccgaaac catcacccag gataccaaca gatttcgcgt gattcagggt ggcttcctgc     2100

atgtggttca tgacggccag gatggaggta gaacgatctg gacccacttc ataagaacga     2160

attgcgaaag tacgacgaaa caccagaccg tgcagaccga agtgatcatc cagcagttga     2220

ggcagttttg gtttcggttt ccactctaga gtcattttat aatcctcctt gtaaacaaaa     2280

ttatttctag agggaaaccg ttgtggaatt gtgagcgctc acaattccac atccacacat     2340

tatacgagcc gatgattaat tgtcaacagc gtcgatcact gtgcatgaag ctcgtaattg     2400

ttatccgctc acaattggat ccaaaatgaa gggaagttcc tatactttct agagaatagg     2460

aacttctata gggagtcgaa taagggcgac acaaaaggta ttctaaatgc ataataaata     2520

ctgataacat cttatagttt gtattatatt ttgtattatc gttgacatgt ataattttga     2580

tatcaaaaac tgattttccc tttattattt tcgagattta ttttcttaat tctctttaac     2640

aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt ttaattatag     2700

gtgttcatca atcgaaaaag caacgtatct tatttaaagt gcgttgcttt tttctcattt     2760

ataaggttaa ataattctca tatatcaagc aaagtgacag gcgcccttaa atattctgac     2820

aaatgctctt tccctaaact ccccccataa aaaaacccgc cgaagcgggt ttttacgtta     2880

tttgcggatt aacgattact cgttatcaga accgcccagg atgcctggca gttccctact     2940

ctcgccgctg cgctcggtcg ttcggctgcg ggacctcagc gctagcggag tgtatactgg     3000

cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa     3060

ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac     3120

tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg     3180

agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag     3240

ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag     3300

tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc     3360

gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt     3420

ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg     3480

tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga     3540

gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag     3600

aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg     3660

actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg     3720

aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa     3780

aacgatctca agaagatcat cttattaatc actgcccgct ttccagtcgg gaaacctgtc     3840

gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg     3900

ccagggtggt ttttcttttc accagtgaga ctggcaacag ctgattgccc ttcaccgcct     3960

ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct     4020

gtttgatggt ggttaacggc gggatataac atgagctatc ttcggtatcg tcgtatccca     4080

ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca     4140

gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt     4200

gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct     4260

gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag     4320

aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca     4380

cgcccagtcg cgtaccgtcc tcatgggaga aaataatact gttgatgggt gtctggtcag     4440

agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct     4500

ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca     4560

ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac     4620

ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca     4680

gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc     4740

ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag     4800

aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact     4860

ctgcgacatc gtataacgtt actggtttca tattcaccac cctgaattga ctctcttccg     4920

ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggcgcgc cgctttt        4977


<210>  4
<211>  882
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(882)
<223>  hGLYAT2 (an amino acid N-acyl transferase) codon optimised for 
       E.coli

<400>  4
atgctggttc tgcacaacag ccaaaaactg caaattctgt acaaatctct ggaaaaatct       60

attcctgaaa gcattaaagt ttatggtgcg atcttcaaca tcaaggataa aaaccctttc      120

aatatggagg tgctggtcga cgcgtggcct gattaccaaa tcgtcatcac ccgtccgcag      180

aagcaagaaa tgaaagatga ccaggaccac tatactaaca cctaccacat cttcactaaa      240

gcgccggaca aactggaaga agttctgagc tactccaatg ttatctcctg ggaacaaacg      300

ctgcagattc aaggttgcca ggaaggtctg gatgaggcga ttcgtaaggt cgctacctct      360

aagtccgttc aagtcgatta catgaaaacc atcctgttta tcccggagct gccgaagaaa      420

cacaaaacct cctctaacga caagatggag ctgttcgaag tagacgatga caacaaagag      480

ggtaactttt ccaatatgtt cctggacgcc tcccacgccg gtctggttaa tgagcactgg      540

gcgttcggta aaaacgaacg ctccctgaag tacattgagc gttgtctgca ggattttctg      600

ggttttggtg tcctgggtcc agagggtcaa ctggtctctt ggatcgttat ggaacagtct      660

tgtgaactgc gtatgggtta tactgtgcca aaataccgtc accaaggtaa tatgctgcaa      720

atcggttatc atctggagaa gtacctgagc cagaaggaaa tcccgttcta tttccatgtt      780

gccgataata acgaaaagag cctgcaagcc ctgaacaatc tgggcttcaa gatctgccct      840

tgcggttggc accagtggaa gtgcacgcct aaaaagtact gt                         882


<210>  5
<211>  864
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(864)
<223>  hGLYAT3 (another amino acid N-acyl transferase) (codon optimised 
       for E.coli)

<400>  5
atgctggttc tgaattgctc tactaaactg ctgatcctgg agaaaatgct gaaatcctgt       60

ttcccggaat ctctgaaagt ttatggtgcg gtaatgaata tcaaccgtgg taatccgttt      120

caaaaagaag ttgttctgga ctcttggcca gatttcaagg cggttatcac ccgtcgtcag      180

cgtgaagcgg aaaccgacaa cctggaccac tataccaacg cgtatgcggt tttctataaa      240

gatgttcgcg cgtaccgtca gctgctggaa gaatgcgatg ttttcaactg ggaccaggtt      300

tttcagatcc aaggtctgca atccgagctg tacgacgttt ctaaggccgt ggcgaactct      360

aaacaactga acatcaagct gacctctttc aaagcggttc atttctctcc ggtatccagc      420

ctgccggaca cctccttcct gaaaggtccg tctccgcgtc tgacctacct gtctgttgcg      480

aatgcggatc tgctgaaccg tacttggtcc cgtggtggca acgaacagtg cctgcgttac      540

attgctaacc tgatctcttg cttcccgagc gtctgcgttc gtgatgaaaa aggtaaccca      600

gtatcttggt ctatcaccga ccagttcgcg accatgtgtc atggctacac cctgcctgaa      660

caccgtcgta agggttactc tcgtctggtt gcgctgaccc tggcgcgtaa gctgcagtct      720

cgtggttttc cgtcccaggg taacgtcctg gacgacaaca ctgcgtccat ctccctgctg      780

aagtccctgc atgccgaatt cctgccgtgc cgtttccacc gtctgattct gaccccagcg      840

accttctctg gtctgccgca cctg                                             864


<210>  6
<211>  1686
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(1686)
<223>  fadD (an acyl-CoA synthetase)

<400>  6
ttgaagaagg tttggcttaa ccgttatccc gcggacgttc cgacggagat caaccctgac       60

cgttatcaat ctctggtaga tatgtttgag cagtcggtcg cgcgctacgc cgatcaacct      120

gcgtttgtga atatggggga ggtaatgacc ttccgcaagc tggaagaacg cagtcgcgcg      180

tttgccgctt atttgcaaca agggttgggg ctgaagaaag gcgatcgcgt tgcgttgatg      240

atgcctaatt tattgcaata tccggtggcg ctgtttggca ttttgcgtgc cgggatgatc      300

gtcgtaaacg ttaacccgtt gtataccccg cgtgagcttg agcatcagct taacgatagc      360

ggcgcatcgg cgattgttat cgtgtctaac tttgctcaca cactggaaaa agtggttgat      420

aaaaccgccg ttcagcacgt aattctgacc cgtatgggcg atcagctatc tacggcaaaa      480

ggcacggtag tcaatttcgt tgttaaatac atcaagcgtt tggtgccgaa ataccatctg      540

ccagatgcca tttcatttcg tagcgcactg cataacggct accggatgca gtacgtcaaa      600

cccgaactgg tgccggaaga tttagctttt ctgcaataca ccggcggcac cactggtgtg      660

gcgaaaggcg cgatgctgac tcaccgcaat atgctggcga acctggaaca ggttaacgcg      720

acctatggtc cgctgttgca tccgggcaaa gagctggtgg tgacggcgct gccgctgtat      780

cacatttttg ccctgaccat taactgcctg ctgtttatcg aactgggtgg gcagaacctg      840

cttatcacta acccgcgcga tattccaggg ttggtaaaag agttagcgaa atatccgttt      900

accgctatca cgggcgttaa caccttgttc aatgcgttgc tgaacaataa agagttccag      960

cagctggatt tctccagtct gcatctttcc gcaggcggtg ggatgccagt gcagcaagtg     1020

gtggcagagc gttgggtgaa actgaccgga cagtatctgc tggaaggcta tggccttacc     1080

gagtgtgcgc cgctggtcag cgttaaccca tatgatattg attatcatag tggtagcatc     1140

ggtttgccgg tgccgtcgac ggaagccaaa ctggtggatg atgatgataa tgaagtacca     1200

ccaggtcaac cgggtgagct ttgtgtcaaa ggaccgcagg tgatgctggg ttactggcag     1260

cgtcccgatg ctaccgatga aatcatcaaa aatggctggt tacacaccgg cgacatcgcg     1320

gtaatggatg aagaaggatt cctgcgcatt gtcgatcgta aaaaagacat gattctggtt     1380

tccggtttta acgtctatcc caacgagatt gaagatgtcg tcatgcagca tcctggcgta     1440

caggaagtcg cggctgttgg cgtaccttcc ggctccagtg gtgaagcggt gaaaatcttc     1500

gtagtgaaaa aagatccatc gcttaccgaa gagtcactgg tgactttttg ccgccgtcag     1560

ctcacgggat acaaagtacc gaagctggtg gagtttcgtg atgagttacc gaaatctaac     1620

gtcggaaaaa ttttgcgacg agaattacgt gacgaagcgc gcggcaaagt ggacaataaa     1680

gcctga                                                                1686


<210>  7
<211>  7138
<212>  DNA
<213>  artificial sequence

<220>
<223>  Vector pCDF[atfA1_Ab(co_Ec)-fadD_Ec]


<220>
<221>  misc_feature
<222>  (1)..(7138)
<223>  Vector pCDF[atfA1_Ab(co_Ec)-fadD_Ec]

<400>  7
cgggatctcg acgctctccc ttatgcgact cctgcgttta gggaaagagc atttgtcaga       60

atatttaagg gcgcctgtca ctttgcttga tatatgagaa ttatttaacc ttataaatga      120

gaaaaaagca acgcacttta aataagatac gttgcttttt cgattgatga acacctataa      180

ttaaactatt catctattat ttatgatttt ttgtatatac aatatttcta gtttgttaaa      240

gagaattaag aaaataaatc tcgaaaataa taaagggaaa atcagttttt gatatcaaaa      300

ttatacatgt caacgataat acaaaatata atacaaacta taagatgtta tcagtattta      360

ttatgcattt agaatacctt ttgtgtcgcc cttattcgac tccctataga agttcctatt      420

ctctagaaag tataggaact tcccttcatt ttggatccaa ttgtgagcgg ataacaatta      480

cgagcttcat gcacagtgat cgacgctgtt gacaattaat catcggctcg tataatgtgt      540

ggatgtggaa ttgtgagcgc tcacaattcc acaacggttt ccctctagaa ataattttgt      600

ttaacaggag gtaaaacata tgaaagcgct gtccccggtg gatcagctgt tcctgtggct      660

ggagaaacgt cagcaaccga tgcatgttgg tggtctgcag ctgtttagct tcccagaggg      720

tgcgggtcct aagtacgtta gcgagttggc tcagcaaatg cgtgactact gtcacccggt      780

ggccccgttc aatcagcgcc tgacccgtcg cctgggtcaa tactattgga cgcgcgacaa      840

acaatttgac attgaccacc attttcgcca tgaagcgctg ccgaagccgg gtcgcattcg      900

cgaactgctg tccttggtta gcgcggagca cagcaatctg ttggaccgcg agcgccctat      960

gtgggaagcg cacctgattg agggcatccg tggccgccaa ttcgctttgt actataagat     1020

ccaccatagc gtgatggatg gcatcagcgc tatgcgcatt gcgtctaaaa cgctgagcac     1080

cgacccgtct gagcgcgaaa tggcaccagc atgggccttc aataccaaga aacgtagccg     1140

tagcctgcct tccaatccag ttgatatggc gagcagcatg gcccgtctga cggcttctat     1200

cagcaaacag gcggcgaccg ttccgggtct ggctcgtgaa gtgtacaagg tcacccagaa     1260

agctaagaag gatgagaact acgtgagcat ttttcaagcg ccggatacca tcttgaacaa     1320

caccattacc ggttcccgtc gttttgcagc acagagcttt ccgctgccgc gtctgaaagt     1380

tatcgcgaag gcgtataact gcaccatcaa caccgtcgtc ctgtctatgt gtggccacgc     1440

gctgcgtgaa tatctgatta gccaacacgc actgccggat gagccgctga tcgcgatggt     1500

gccgatgagc ctgcgtcagg acgacagcac gggtggtaat cagattggca tgatcctggc     1560

caacctgggc acccatatct gcgatccggc gaatcgtttg cgtgttattc acgactccgt     1620

cgaggaagcg aaatcgcgtt tcagccagat gagcccggag gagatcctga actttaccgc     1680

actgactatg gccccgacgg gcttgaatct gctgaccggc ctggcaccga aatggcgtgc     1740

gttcaacgtc gtcattagca acattccggg tccgaaggaa ccgctgtatt ggaatggcgc     1800

ccagctgcaa ggtgtgtacc cggtaagcat tgcgttggac cgtattgcac tgaacatcac     1860

gctgactagc tacgtggatc agatggagtt cggtctgatc gcatgccgtc gcacgctgcc     1920

gagcatgcaa cgtctgctgg attatctgga acaaagcatt cgtgagttgg aaatcggtgc     1980

cggcatcaag taagcgttaa gtctgagagg tgaagaattg aagaaggttt ggcttaaccg     2040

ttatcccgcg gacgttccga cggagatcaa ccctgaccgt tatcaatctc tggtagatat     2100

gtttgagcag tcggtcgcgc gctacgccga tcaacctgcg tttgtgaata tgggggaggt     2160

aatgaccttc cgcaagctgg aagaacgcag tcgcgcgttt gccgcttatt tgcaacaagg     2220

gttggggctg aagaaaggcg atcgcgttgc gttgatgatg cctaatttat tgcaatatcc     2280

ggtggcgctg tttggcattt tgcgtgccgg gatgatcgtc gtaaacgtta acccgttgta     2340

taccccgcgt gagcttgagc atcagcttaa cgatagcggc gcatcggcga ttgttatcgt     2400

gtctaacttt gctcacacac tggaaaaagt ggttgataaa accgccgttc agcacgtaat     2460

tctgacccgt atgggcgatc agctatctac ggcaaaaggc acggtagtca atttcgttgt     2520

taaatacatc aagcgtttgg tgccgaaata ccatctgcca gatgccattt catttcgtag     2580

cgcactgcat aacggctacc ggatgcagta cgtcaaaccc gaactggtgc cggaagattt     2640

agcttttctg caatacaccg gcggcaccac tggtgtggcg aaaggcgcga tgctgactca     2700

ccgcaatatg ctggcgaacc tggaacaggt taacgcgacc tatggtccgc tgttgcatcc     2760

gggcaaagag ctggtggtga cggcgctgcc gctgtatcac atttttgccc tgaccattaa     2820

ctgcctgctg tttatcgaac tgggtgggca gaacctgctt atcactaacc cgcgcgatat     2880

tccagggttg gtaaaagagt tagcgaaata tccgtttacc gctatcacgg gcgttaacac     2940

cttgttcaat gcgttgctga acaataaaga gttccagcag ctggatttct ccagtctgca     3000

tctttccgca ggcggtggga tgccagtgca gcaagtggtg gcagagcgtt gggtgaaact     3060

gaccggacag tatctgctgg aaggctatgg ccttaccgag tgtgcgccgc tggtcagcgt     3120

taacccatat gatattgatt atcatagtgg tagcatcggt ttgccggtgc cgtcgacgga     3180

agccaaactg gtggatgatg atgataatga agtaccacca ggtcaaccgg gtgagctttg     3240

tgtcaaagga ccgcaggtga tgctgggtta ctggcagcgt cccgatgcta ccgatgaaat     3300

catcaaaaat ggctggttac acaccggcga catcgcggta atggatgaag aaggattcct     3360

gcgcattgtc gatcgtaaaa aagacatgat tctggtttcc ggttttaacg tctatcccaa     3420

cgagattgaa gatgtcgtca tgcagcatcc tggcgtacag gaagtcgcgg ctgttggcgt     3480

accttccggc tccagtggtg aagcggtgaa aatcttcgta gtgaaaaaag atccatcgct     3540

taccgaagag tcactggtga ctttttgccg ccgtcagctc acgggataca aagtaccgaa     3600

gctggtggag tttcgtgatg agttaccgaa atctaacgtc ggaaaaattt tgcgacgaga     3660

attacgtgac gaagcgcgcg gcaaagtgga caataaagcc tgagcgttaa gtcagtcgtc     3720

agacgccggt taatccggcg ttttttttga cgcccactaa agagaaaaca atctcgagtc     3780

tggtaaagaa accgctgctg cgaaatttga acgccagcac atggactcgt ctactagcgc     3840

agcttaatta acctaggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc     3900

ctctaaacgg gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt     3960

cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta     4020

acgaccctgc cctgaaccga cgaccgggtc atcgtggccg gatcttgcgg cccctcggct     4080

tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg     4140

gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata     4200

agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca     4260

gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga     4320

caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt     4380

taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc     4440

cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag     4500

atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc     4560

tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac     4620

aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa     4680

aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag     4740

caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac     4800

ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt     4860

cgatacttcg gcgatcaccg cttccctcat actcttcctt tttcaatatt attgaagcat     4920

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca     4980

aatagctagc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac     5040

acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac     5100

agcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata     5160

aacagacgct tttccggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt     5220

acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggcgggtc gctccctctt     5280

gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt ctcccttacg     5340

ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc     5400

gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg     5460

gtaactgttc acttgagtcc aacccggaaa agcacggtaa aacgccactg gcagcagcca     5520

ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg     5580

tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca     5640

gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg     5700

tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc     5760

ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct tcaaatgtag     5820

cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca tgccccgcgc     5880

ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc     5940

ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg     6000

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     6060

tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct     6120

tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc     6180

gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt     6240

cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta atggcgcgca     6300

ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat     6360

tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg     6420

ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg     6480

ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca     6540

gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg     6600

tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa     6660

tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa     6720

gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca     6780

cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt     6840

gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt     6900

gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg     6960

ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac     7020

cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac     7080

tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtc       7138


<210>  8
<211>  6649
<212>  DNA
<213>  artificial sequence

<220>
<223>  Vector pCDF{Ptac}[hGLYAT2(co_Ec)-fadD_Ec]


<220>
<221>  misc_feature
<222>  (1)..(6649)
<223>  Vector pCDF{Ptac}[hGLYAT2(co_Ec)-fadD_Ec]

<400>  8
cgggatctcg acgctctccc ttatgcgact cctgcgttta gggaaagagc atttgtcaga       60

atatttaagg gcgcctgtca ctttgcttga tatatgagaa ttatttaacc ttataaatga      120

gaaaaaagca acgcacttta aataagatac gttgcttttt cgattgatga acacctataa      180

ttaaactatt catctattat ttatgatttt ttgtatatac aatatttcta gtttgttaaa      240

gagaattaag aaaataaatc tcgaaaataa taaagggaaa atcagttttt gatatcaaaa      300

ttatacatgt caacgataat acaaaatata atacaaacta taagatgtta tcagtattta      360

ttatgcattt agaatacctt ttgtgtcgcc cttattcgac tccctataga agttcctatt      420

ctctagaaag tataggaact tcccttcatt ttggatccaa ttgtgagcgg ataacaatta      480

cgagcttcat gcacagtgat cgacgctgtt gacaattaat catcggctcg tataatgtgt      540

ggatgtggaa ttgtgagcgc tcacaattcc acaacggttt ccctctagaa ataattttgt      600

ttaacaggag gtaaaacata tgctggttct gcacaacagc caaaaactgc aaattctgta      660

caaatctctg gaaaaatcta ttcctgaaag cattaaagtt tatggtgcga tcttcaacat      720

caaggataaa aaccctttca atatggaggt gctggtcgac gcgtggcctg attaccaaat      780

cgtcatcacc cgtccgcaga agcaagaaat gaaagatgac caggaccact atactaacac      840

ctaccacatc ttcactaaag cgccggacaa actggaagaa gttctgagct actccaatgt      900

tatctcctgg gaacaaacgc tgcagattca aggttgccag gaaggtctgg atgaggcgat      960

tcgtaaggtc gctacctcta agtccgttca agtcgattac atgaaaacca tcctgtttat     1020

cccggagctg ccgaagaaac acaaaacctc ctctaacgac aagatggagc tgttcgaagt     1080

agacgatgac aacaaagagg gtaacttttc caatatgttc ctggacgcct cccacgccgg     1140

tctggttaat gagcactggg cgttcggtaa aaacgaacgc tccctgaagt acattgagcg     1200

ttgtctgcag gattttctgg gttttggtgt cctgggtcca gagggtcaac tggtctcttg     1260

gatcgttatg gaacagtctt gtgaactgcg tatgggttat actgtgccaa aataccgtca     1320

ccaaggtaat atgctgcaaa tcggttatca tctggagaag tacctgagcc agaaggaaat     1380

cccgttctat ttccatgttg ccgataataa cgaaaagagc ctgcaagccc tgaacaatct     1440

gggcttcaag atctgccctt gcggttggca ccagtggaag tgcacgccta aaaagtactg     1500

ttaagcgtta agtctgagag gtgaagaatt gaagaaggtt tggcttaacc gttatcccgc     1560

ggacgttccg acggagatca accctgaccg ttatcaatct ctggtagata tgtttgagca     1620

gtcggtcgcg cgctacgccg atcaacctgc gtttgtgaat atgggggagg taatgacctt     1680

ccgcaagctg gaagaacgca gtcgcgcgtt tgccgcttat ttgcaacaag ggttggggct     1740

gaagaaaggc gatcgcgttg cgttgatgat gcctaattta ttgcaatatc cggtggcgct     1800

gtttggcatt ttgcgtgccg ggatgatcgt cgtaaacgtt aacccgttgt ataccccgcg     1860

tgagcttgag catcagctta acgatagcgg cgcatcggcg attgttatcg tgtctaactt     1920

tgctcacaca ctggaaaaag tggttgataa aaccgccgtt cagcacgtaa ttctgacccg     1980

tatgggcgat cagctatcta cggcaaaagg cacggtagtc aatttcgttg ttaaatacat     2040

caagcgtttg gtgccgaaat accatctgcc agatgccatt tcatttcgta gcgcactgca     2100

taacggctac cggatgcagt acgtcaaacc cgaactggtg ccggaagatt tagcttttct     2160

gcaatacacc ggcggcacca ctggtgtggc gaaaggcgcg atgctgactc accgcaatat     2220

gctggcgaac ctggaacagg ttaacgcgac ctatggtccg ctgttgcatc cgggcaaaga     2280

gctggtggtg acggcgctgc cgctgtatca catttttgcc ctgaccatta actgcctgct     2340

gtttatcgaa ctgggtgggc agaacctgct tatcactaac ccgcgcgata ttccagggtt     2400

ggtaaaagag ttagcgaaat atccgtttac cgctatcacg ggcgttaaca ccttgttcaa     2460

tgcgttgctg aacaataaag agttccagca gctggatttc tccagtctgc atctttccgc     2520

aggcggtggg atgccagtgc agcaagtggt ggcagagcgt tgggtgaaac tgaccggaca     2580

gtatctgctg gaaggctatg gccttaccga gtgtgcgccg ctggtcagcg ttaacccata     2640

tgatattgat tatcatagtg gtagcatcgg tttgccggtg ccgtcgacgg aagccaaact     2700

ggtggatgat gatgataatg aagtaccacc aggtcaaccg ggtgagcttt gtgtcaaagg     2760

accgcaggtg atgctgggtt actggcagcg tcccgatgct accgatgaaa tcatcaaaaa     2820

tggctggtta cacaccggcg acatcgcggt aatggatgaa gaaggattcc tgcgcattgt     2880

cgatcgtaaa aaagacatga ttctggtttc cggttttaac gtctatccca acgagattga     2940

agatgtcgtc atgcagcatc ctggcgtaca ggaagtcgcg gctgttggcg taccttccgg     3000

ctccagtggt gaagcggtga aaatcttcgt agtgaaaaaa gatccatcgc ttaccgaaga     3060

gtcactggtg actttttgcc gccgtcagct cacgggatac aaagtaccga agctggtgga     3120

gtttcgtgat gagttaccga aatctaacgt cggaaaaatt ttgcgacgag aattacgtga     3180

cgaagcgcgc ggcaaagtgg acaataaagc ctgagcgtta agtcagtcgt cagacgccgg     3240

ttaatccggc gttttttttg acgcccacta aagagaaaac aatctcgagt ctggtaaaga     3300

aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt     3360

aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg     3420

ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct     3480

tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg     3540

ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc ttgaacgaat     3600

tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc     3660

ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct     3720

agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc     3780

gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag     3840

cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc     3900

atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg     3960

acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc     4020

gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg     4080

cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac     4140

ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt     4200

gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat     4260

atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa     4320

cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc     4380

ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca tttatcaggg     4440

ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaatagctag     4500

ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa     4560

gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa cagcagggcc     4620

gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat aaacagacgc     4680

ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag tacgggcgaa     4740

acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct tgcgctctcc     4800

tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac gggaagtgtg     4860

gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt cgctccaagc     4920

tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt     4980

cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc attggtaact     5040

gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa     5100

gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg     5160

gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt     5220

cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     5280

tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag     5340

tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa     5400

ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt     5460

gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc     5520

gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg     5580

ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct     5640

ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct     5700

gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca     5760

ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca     5820

gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt     5880

gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct     5940

gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag     6000

aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca     6060

cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag     6120

agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct     6180

ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca     6240

ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac     6300

ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca     6360

gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc     6420

ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag     6480

aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact     6540

ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg     6600

ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtc                 6649


<210>  9
<211>  6631
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pCDF{Ptac}[hGLYAT3(co_Ec)-fadD_Ec]


<220>
<221>  misc_feature
<222>  (1)..(6631)

<400>  9
cgggatctcg acgctctccc ttatgcgact cctgcgttta gggaaagagc atttgtcaga       60

atatttaagg gcgcctgtca ctttgcttga tatatgagaa ttatttaacc ttataaatga      120

gaaaaaagca acgcacttta aataagatac gttgcttttt cgattgatga acacctataa      180

ttaaactatt catctattat ttatgatttt ttgtatatac aatatttcta gtttgttaaa      240

gagaattaag aaaataaatc tcgaaaataa taaagggaaa atcagttttt gatatcaaaa      300

ttatacatgt caacgataat acaaaatata atacaaacta taagatgtta tcagtattta      360

ttatgcattt agaatacctt ttgtgtcgcc cttattcgac tccctataga agttcctatt      420

ctctagaaag tataggaact tcccttcatt ttggatccaa ttgtgagcgg ataacaatta      480

cgagcttcat gcacagtgat cgacgctgtt gacaattaat catcggctcg tataatgtgt      540

ggatgtggaa ttgtgagcgc tcacaattcc acaacggttt ccctctagaa ataattttgt      600

ttaacaggag gtaaaacata tgctggttct gaattgctct actaaactgc tgatcctgga      660

gaaaatgctg aaatcctgtt tcccggaatc tctgaaagtt tatggtgcgg taatgaatat      720

caaccgtggt aatccgtttc aaaaagaagt tgttctggac tcttggccag atttcaaggc      780

ggttatcacc cgtcgtcagc gtgaagcgga aaccgacaac ctggaccact ataccaacgc      840

gtatgcggtt ttctataaag atgttcgcgc gtaccgtcag ctgctggaag aatgcgatgt      900

tttcaactgg gaccaggttt ttcagatcca aggtctgcaa tccgagctgt acgacgtttc      960

taaggccgtg gcgaactcta aacaactgaa catcaagctg acctctttca aagcggttca     1020

tttctctccg gtatccagcc tgccggacac ctccttcctg aaaggtccgt ctccgcgtct     1080

gacctacctg tctgttgcga atgcggatct gctgaaccgt acttggtccc gtggtggcaa     1140

cgaacagtgc ctgcgttaca ttgctaacct gatctcttgc ttcccgagcg tctgcgttcg     1200

tgatgaaaaa ggtaacccag tatcttggtc tatcaccgac cagttcgcga ccatgtgtca     1260

tggctacacc ctgcctgaac accgtcgtaa gggttactct cgtctggttg cgctgaccct     1320

ggcgcgtaag ctgcagtctc gtggttttcc gtcccagggt aacgtcctgg acgacaacac     1380

tgcgtccatc tccctgctga agtccctgca tgccgaattc ctgccgtgcc gtttccaccg     1440

tctgattctg accccagcga ccttctctgg tctgccgcac ctgtaagcgt taagtctgag     1500

aggtgaagaa ttgaagaagg tttggcttaa ccgttatccc gcggacgttc cgacggagat     1560

caaccctgac cgttatcaat ctctggtaga tatgtttgag cagtcggtcg cgcgctacgc     1620

cgatcaacct gcgtttgtga atatggggga ggtaatgacc ttccgcaagc tggaagaacg     1680

cagtcgcgcg tttgccgctt atttgcaaca agggttgggg ctgaagaaag gcgatcgcgt     1740

tgcgttgatg atgcctaatt tattgcaata tccggtggcg ctgtttggca ttttgcgtgc     1800

cgggatgatc gtcgtaaacg ttaacccgtt gtataccccg cgtgagcttg agcatcagct     1860

taacgatagc ggcgcatcgg cgattgttat cgtgtctaac tttgctcaca cactggaaaa     1920

agtggttgat aaaaccgccg ttcagcacgt aattctgacc cgtatgggcg atcagctatc     1980

tacggcaaaa ggcacggtag tcaatttcgt tgttaaatac atcaagcgtt tggtgccgaa     2040

ataccatctg ccagatgcca tttcatttcg tagcgcactg cataacggct accggatgca     2100

gtacgtcaaa cccgaactgg tgccggaaga tttagctttt ctgcaataca ccggcggcac     2160

cactggtgtg gcgaaaggcg cgatgctgac tcaccgcaat atgctggcga acctggaaca     2220

ggttaacgcg acctatggtc cgctgttgca tccgggcaaa gagctggtgg tgacggcgct     2280

gccgctgtat cacatttttg ccctgaccat taactgcctg ctgtttatcg aactgggtgg     2340

gcagaacctg cttatcacta acccgcgcga tattccaggg ttggtaaaag agttagcgaa     2400

atatccgttt accgctatca cgggcgttaa caccttgttc aatgcgttgc tgaacaataa     2460

agagttccag cagctggatt tctccagtct gcatctttcc gcaggcggtg ggatgccagt     2520

gcagcaagtg gtggcagagc gttgggtgaa actgaccgga cagtatctgc tggaaggcta     2580

tggccttacc gagtgtgcgc cgctggtcag cgttaaccca tatgatattg attatcatag     2640

tggtagcatc ggtttgccgg tgccgtcgac ggaagccaaa ctggtggatg atgatgataa     2700

tgaagtacca ccaggtcaac cgggtgagct ttgtgtcaaa ggaccgcagg tgatgctggg     2760

ttactggcag cgtcccgatg ctaccgatga aatcatcaaa aatggctggt tacacaccgg     2820

cgacatcgcg gtaatggatg aagaaggatt cctgcgcatt gtcgatcgta aaaaagacat     2880

gattctggtt tccggtttta acgtctatcc caacgagatt gaagatgtcg tcatgcagca     2940

tcctggcgta caggaagtcg cggctgttgg cgtaccttcc ggctccagtg gtgaagcggt     3000

gaaaatcttc gtagtgaaaa aagatccatc gcttaccgaa gagtcactgg tgactttttg     3060

ccgccgtcag ctcacgggat acaaagtacc gaagctggtg gagtttcgtg atgagttacc     3120

gaaatctaac gtcggaaaaa ttttgcgacg agaattacgt gacgaagcgc gcggcaaagt     3180

ggacaataaa gcctgagcgt taagtcagtc gtcagacgcc ggttaatccg gcgttttttt     3240

tgacgcccac taaagagaaa acaatctcga gtctggtaaa gaaaccgctg ctgcgaaatt     3300

tgaacgccag cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac     3360

cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt     3420

gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc     3480

ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg     3540

gtcatcgtgg ccggatcttg cggcccctcg gcttgaacga attgttagac attatttgcc     3600

gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg     3660

cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg     3720

ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat     3780

tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc     3840

gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag     3900

atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct     3960

atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa     4020

gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg     4080

ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat     4140

ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt     4200

tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag     4260

gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg     4320

ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct     4380

catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg     4440

atacatattt gaatgtattt agaaaaataa acaaatagct agctcactcg gtcgctacgc     4500

tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt     4560

ggataagcag gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc     4620

cataggctcc gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg     4680

ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag     4740

atccccaccg tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt     4800

taccggatac ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca     4860

cacactggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac     4920

tccccgttca gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg     4980

aaaagcacgg taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt     5040

tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag     5100

gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac     5160

tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga     5220

ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgctct     5280

agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata     5340

agttgtaatt ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa     5400

ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat     5460

tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg     5520

aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt     5580

tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca     5640

gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg     5700

gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac     5760

caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg     5820

caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac     5880

cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga     5940

gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta     6000

acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt     6060

cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg     6120

ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt     6180

taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt     6240

cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag     6300

atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc     6360

caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca     6420

gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt     6480

tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg     6540

ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac     6600

cgcgaaaggt tttgcgccat tcgatggtgt c                                    6631


<210>  10
<211>  693
<212>  DNA
<213>  Pseudomonas putida


<220>
<221>  misc_feature
<222>  (1)..(693)
<223>  alkL (an importer facilitating transport of hydrophobic acyl 
       across cell membranes) gene

<400>  10
gtgagttttt ctaattataa agtaatcgcg atgccggtgt tggttgctaa ttttgttttg       60

ggggcggcca ctgcatgggc gaatgaaaat tatccggcga aatctgctgg ctataatcag      120

ggtgactggg tcgctagctt caatttttct aaggtctatg tgggtgagga gcttggcgat      180

ctaaatgttg gagggggggc tttgccaaat gctgatgtaa gtattggtaa tgatacaaca      240

cttacgtttg atatcgccta ttttgttagc tcaaatatag cggtggattt ttttgttggg      300

gtgccagcta gggctaaatt tcaaggtgag aaatcaatct cctcgctggg aagagtcagt      360

gaagttgatt acggccctgc aattctttcg cttcaatatc attacgatag ctttgagcga      420

ctttatccat atgttggggt tggtgttggt cgggtgctat tttttgataa aaccgacggt      480

gctttgagtt cgtttgatat taaggataaa tgggcgcctg cttttcaggt tggccttaga      540

tatgaccttg gtaactcatg gatgctaaat tcagatgtgc gttatattcc tttcaaaacg      600

gacgtcacag gtactcttgg cccggttcct gtttctacta aaattgaggt tgatcctttc      660

attctcagtc ttggtgcgtc atatgttttc taa                                   693


<210>  11
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lacuv5 promoter


<220>
<221>  misc_feature
<222>  (1)..(153)

<400>  11
ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc ccaggcttga       60

cactttatgc ttccggctcg tataatgtgt ggaattgtga gcggataaca ataacaattt      120

cacacaggat ctaggaacca aggagagtgg cat                                   153


<210>  12
<211>  4876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pCDF[alkLmod1]


<220>
<221>  misc_feature
<222>  (1)..(4876)
<223>  Vector pCDF[alkLmod1]

<400>  12
ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag       60

gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cttcaatatt      120

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga      180

aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac attatcgcga      240

gcccatttat acctgaatat ggctcataac accccttgtt tgcctggcgg cagtagcgcg      300

gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt      360

gtggggactc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca      420

gtcgaaagac tgggcctttc gcccgggcta attatggggt gtcgccctta ttcgactcta      480

tagtgaagtt cctattctct agaaagtata ggaacttctg aagtggggat ccggccggcc      540

tgcagccccg cagggcctgt ctcggtcgat cattcagccc ggctcataga tatgcgggca      600

gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccccag gcttgacact      660

ttatgcttcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaataa caatttcaca      720

caggatctag gaaccaagga gagtggcatg tgagtttttc taattataaa gtaatcgcga      780

tgccggtgtt ggttgctaat tttgttttgg gggcggccac tgcatgggcg aatgaaaatt      840

atccggcgaa atctgctggc tataatcagg gtgactgggt cgctagcttc aatttttcta      900

aggtctatgt gggtgaggag cttggcgatc taaatgttgg agggggggct ttgccaaatg      960

ctgatgtaag tattggtaat gatacaacac ttacgtttga tatcgcctat tttgttagct     1020

caaatatagc ggtggatttt tttgttgggg tgccagctag ggctaaattt caaggtgaga     1080

aatcaatctc ctcgctggga agagtcagtg aagttgatta cggccctgca attctttcgc     1140

ttcaatatca ttacgatagc tttgagcgac tttatccata tgttggggtt ggtgttggtc     1200

gggtgctatt ttttgataaa accgacggtg ctttgagttc gtttgatatt aaggataaat     1260

gggcgcctgc ttttcaggtt ggccttagat atgaccttgg taactcatgg atgctaaatt     1320

cagatgtgcg ttatattcct ttcaaaacgg acgtcacagg tactcttggc ccggttcctg     1380

tttctactaa aattgaggtt gatcctttca ttctcagtct tggtgcgtca tatgttttct     1440

aaggtaccct cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg     1500

actcgtctac tagcgcagct taattaacct aggctgctgc caccgctgag caataactag     1560

cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat     1620

ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga     1680

cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcatcg tggccggatc     1740

ttgcggcccc tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct     1800

cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt     1860

cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca     1920

ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc     1980

tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg     2040

gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg     2100

gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg     2160

tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt     2220

cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga     2280

tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg     2340

aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta     2400

cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg     2460

agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta     2520

cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatactc ttcctttttc     2580

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     2640

tttagaaaaa taaacaaata gctagctcac tcggtcgcta cgctccgggc gtgagactgc     2700

ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag caggggacta     2760

acatgtgagg caaaacagca gggccgcgcc ggtggcgttt ttccataggc tccgccctcc     2820

tgccagagtt cacataaaca gacgcttttc cggtgcatct gtgggagccg tgaggctcaa     2880

ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca ccgtttccgg     2940

cgggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgttcc     3000

gcctttctcc cttacgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc     3060

tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac     3120

tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca cggtaaaacg     3180

ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag ctaaacacgc     3240

ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat ttggttgctg     3300

tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta accttcgatc     3360

aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg cagaaaaaaa     3420

ggatctcaag aagatccttt gatcttttct actgaaccgc tctagatttc agtgcaattt     3480

atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt     3540

tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg     3600

gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc     3660

ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg     3720

ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc     3780

aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg     3840

gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag     3900

ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac     3960

tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg     4020

ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag     4080

tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca     4140

gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg     4200

tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata     4260

atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag     4320

gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg     4380

acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct     4440

accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca     4500

atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt     4560

ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct     4620

tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg     4680

gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc     4740

accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc     4800

cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt     4860

aatacgactc actata                                                     4876


<210>  13
<211>  7468
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pCDF{Ptac}[hGLYAT2(co_Ec)-fadD_Ec]{Placuv5}[alkLmod1]


<220>
<221>  misc_feature
<222>  (1)..(7468)
<223>  Vector pCDF{Ptac}[hGLYAT2(co_Ec)-fadD_Ec]{Placuv5}[alkLmod1]

<400>  13
cgggatctcg acgctctccc ttatgcgact cctgcgttta gggaaagagc atttgtcaga       60

atatttaagg gcgcctgtca ctttgcttga tatatgagaa ttatttaacc ttataaatga      120

gaaaaaagca acgcacttta aataagatac gttgcttttt cgattgatga acacctataa      180

ttaaactatt catctattat ttatgatttt ttgtatatac aatatttcta gtttgttaaa      240

gagaattaag aaaataaatc tcgaaaataa taaagggaaa atcagttttt gatatcaaaa      300

ttatacatgt caacgataat acaaaatata atacaaacta taagatgtta tcagtattta      360

ttatgcattg tctcggtcga tcattcagcc cggctcatag atatgcgggc agtgagcgca      420

acgcaattaa tgtaagttag ctcactcatt aggcacccca ggcttgacac tttatgcttc      480

cggctcgtat aatgtgtgga attgtgagcg gataacaata acaatttcac acaggatcta      540

ggaaccaagg agagtggcat gtgagttttt ctaattataa agtaatcgcg atgccggtgt      600

tggttgctaa ttttgttttg ggggcggcca ctgcatgggc gaatgaaaat tatccggcga      660

aatctgctgg ctataatcag ggtgactggg tcgctagctt caatttttct aaggtctatg      720

tgggtgagga gcttggcgat ctaaatgttg gagggggggc tttgccaaat gctgatgtaa      780

gtattggtaa tgatacaaca cttacgtttg atatcgccta ttttgttagc tcaaatatag      840

cggtggattt ttttgttggg gtgccagcta gggctaaatt tcaaggtgag aaatcaatct      900

cctcgctggg aagagtcagt gaagttgatt acggccctgc aattctttcg cttcaatatc      960

attacgatag ctttgagcga ctttatccat atgttggggt tggtgttggt cgggtgctat     1020

tttttgataa aaccgacggt gctttgagtt cgtttgatat taaggataaa tgggcgcctg     1080

cttttcaggt tggccttaga tatgaccttg gtaactcatg gatgctaaat tcagatgtgc     1140

gttatattcc tttcaaaacg gacgtcacag gtactcttgg cccggttcct gtttctacta     1200

aaattgaggt tgatcctttc attctcagtc ttggtgcgtc atatgttttc taaggtaccc     1260

tcgagtctgg tggatccaat tgtgagcgga taacaattac gagcttcatg cacagtgatc     1320

gacgctgttg acaattaatc atcggctcgt ataatgtgtg gatgtggaat tgtgagcgct     1380

cacaattcca caacggtttc cctctagaaa taattttgtt taacaggagg taaaacatat     1440

gctggttctg cacaacagcc aaaaactgca aattctgtac aaatctctgg aaaaatctat     1500

tcctgaaagc attaaagttt atggtgcgat cttcaacatc aaggataaaa accctttcaa     1560

tatggaggtg ctggtcgacg cgtggcctga ttaccaaatc gtcatcaccc gtccgcagaa     1620

gcaagaaatg aaagatgacc aggaccacta tactaacacc taccacatct tcactaaagc     1680

gccggacaaa ctggaagaag ttctgagcta ctccaatgtt atctcctggg aacaaacgct     1740

gcagattcaa ggttgccagg aaggtctgga tgaggcgatt cgtaaggtcg ctacctctaa     1800

gtccgttcaa gtcgattaca tgaaaaccat cctgtttatc ccggagctgc cgaagaaaca     1860

caaaacctcc tctaacgaca agatggagct gttcgaagta gacgatgaca acaaagaggg     1920

taacttttcc aatatgttcc tggacgcctc ccacgccggt ctggttaatg agcactgggc     1980

gttcggtaaa aacgaacgct ccctgaagta cattgagcgt tgtctgcagg attttctggg     2040

ttttggtgtc ctgggtccag agggtcaact ggtctcttgg atcgttatgg aacagtcttg     2100

tgaactgcgt atgggttata ctgtgccaaa ataccgtcac caaggtaata tgctgcaaat     2160

cggttatcat ctggagaagt acctgagcca gaaggaaatc ccgttctatt tccatgttgc     2220

cgataataac gaaaagagcc tgcaagccct gaacaatctg ggcttcaaga tctgcccttg     2280

cggttggcac cagtggaagt gcacgcctaa aaagtactgt taagcgttaa gtctgagagg     2340

tgaagaattg aagaaggttt ggcttaaccg ttatcccgcg gacgttccga cggagatcaa     2400

ccctgaccgt tatcaatctc tggtagatat gtttgagcag tcggtcgcgc gctacgccga     2460

tcaacctgcg tttgtgaata tgggggaggt aatgaccttc cgcaagctgg aagaacgcag     2520

tcgcgcgttt gccgcttatt tgcaacaagg gttggggctg aagaaaggcg atcgcgttgc     2580

gttgatgatg cctaatttat tgcaatatcc ggtggcgctg tttggcattt tgcgtgccgg     2640

gatgatcgtc gtaaacgtta acccgttgta taccccgcgt gagcttgagc atcagcttaa     2700

cgatagcggc gcatcggcga ttgttatcgt gtctaacttt gctcacacac tggaaaaagt     2760

ggttgataaa accgccgttc agcacgtaat tctgacccgt atgggcgatc agctatctac     2820

ggcaaaaggc acggtagtca atttcgttgt taaatacatc aagcgtttgg tgccgaaata     2880

ccatctgcca gatgccattt catttcgtag cgcactgcat aacggctacc ggatgcagta     2940

cgtcaaaccc gaactggtgc cggaagattt agcttttctg caatacaccg gcggcaccac     3000

tggtgtggcg aaaggcgcga tgctgactca ccgcaatatg ctggcgaacc tggaacaggt     3060

taacgcgacc tatggtccgc tgttgcatcc gggcaaagag ctggtggtga cggcgctgcc     3120

gctgtatcac atttttgccc tgaccattaa ctgcctgctg tttatcgaac tgggtgggca     3180

gaacctgctt atcactaacc cgcgcgatat tccagggttg gtaaaagagt tagcgaaata     3240

tccgtttacc gctatcacgg gcgttaacac cttgttcaat gcgttgctga acaataaaga     3300

gttccagcag ctggatttct ccagtctgca tctttccgca ggcggtggga tgccagtgca     3360

gcaagtggtg gcagagcgtt gggtgaaact gaccggacag tatctgctgg aaggctatgg     3420

ccttaccgag tgtgcgccgc tggtcagcgt taacccatat gatattgatt atcatagtgg     3480

tagcatcggt ttgccggtgc cgtcgacgga agccaaactg gtggatgatg atgataatga     3540

agtaccacca ggtcaaccgg gtgagctttg tgtcaaagga ccgcaggtga tgctgggtta     3600

ctggcagcgt cccgatgcta ccgatgaaat catcaaaaat ggctggttac acaccggcga     3660

catcgcggta atggatgaag aaggattcct gcgcattgtc gatcgtaaaa aagacatgat     3720

tctggtttcc ggttttaacg tctatcccaa cgagattgaa gatgtcgtca tgcagcatcc     3780

tggcgtacag gaagtcgcgg ctgttggcgt accttccggc tccagtggtg aagcggtgaa     3840

aatcttcgta gtgaaaaaag atccatcgct taccgaagag tcactggtga ctttttgccg     3900

ccgtcagctc acgggataca aagtaccgaa gctggtggag tttcgtgatg agttaccgaa     3960

atctaacgtc ggaaaaattt tgcgacgaga attacgtgac gaagcgcgcg gcaaagtgga     4020

caataaagcc tgagcgttaa gtcagtcgtc agacgccggt taatccggcg ttttttttga     4080

cgcccactaa agagaaaaca atctcgagtc tggtaaagaa accgctgctg cgaaatttga     4140

acgccagcac atggactcgt ctactagcgc agcttaatta acctaggctg ctgccaccgc     4200

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct     4260

gaaacctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt     4320

aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc     4380

atcgtggccg gatcttgcgg cccctcggct tgaacgaatt gttagacatt atttgccgac     4440

taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga     4500

ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg     4560

atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt     4620

gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc     4680

agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc     4740

ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg     4800

ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat     4860

acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata     4920

acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc     4980

gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt     5040

ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc     5100

gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc     5160

gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat     5220

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     5280

catatttgaa tgtatttaga aaaataaaca aatagctagc tcactcggtc gctacgctcc     5340

gggcgtgaga ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgga     5400

taagcagggg actaacatgt gaggcaaaac agcagggccg cgccggtggc gtttttccat     5460

aggctccgcc ctcctgccag agttcacata aacagacgct tttccggtgc atctgtggga     5520

gccgtgaggc tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc     5580

cccaccgttt ccggcgggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac     5640

cggatacctg ttccgccttt ctcccttacg ggaagtgtgg cgctttctca tagctcacac     5700

actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaactcc     5760

ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccggaaa     5820

agcacggtaa aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt     5880

ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac     5940

agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga     6000

cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta     6060

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgctctaga     6120

tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt     6180

tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc     6240

tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc     6300

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     6360

cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca     6420

ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca     6480

agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg     6540

ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagatg tccgcaccaa     6600

cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa     6660

ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg     6720

acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat     6780

atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca     6840

gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt     6900

catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg     6960

gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa     7020

tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga     7080

cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt     7140

taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa     7200

tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct     7260

ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca     7320

ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta     7380

ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc     7440

gaaaggtttt gcgccattcg atggtgtc                                        7468


<210>  14
<211>  5367
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pET-28b


<220>
<221>  misc_feature
<222>  (1)..(5367)
<223>  Vector pET-28b

<400>  14
ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc       60

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc      120

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg      180

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca      240

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc      300

tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct      360

tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa      420

caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     2160

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     2220

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt     2280

attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac     2340

aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg     2400

gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     2460

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     2520

ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg     2580

tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga     2640

agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg     2700

gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa     2760

cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt     2820

tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt     2880

caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct     2940

gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac     3000

gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag     3060

cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc     3120

cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc     3180

atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag     3240

gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg     3300

ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg     3360

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac     3420

cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa     3480

tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac     3540

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     3600

gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac     3660

cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa     3720

atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta     3780

tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc     3840

gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag     3900

catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat     3960

cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga     4020

gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg     4080

ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg     4140

gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc     4200

atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt     4260

gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct     4320

ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag     4380

ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc     4440

cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt     4500

cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc     4560

atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc     4620

ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat     4680

ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc     4740

cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc     4800

cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc     4860

gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg     4920

cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa     4980

attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat     5040

tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca     5100

gcagcggcct ggtgccgcgc ggcagccata tggctagcat gactggtgga cagcaaatgg     5160

gtcgggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc     5220

accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca     5280

ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt     5340

tgctgaaagg aggaactata tccggat                                         5367


<210>  15
<211>  6432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pET-28b{Ptac}[hGLYAT2(co_Ec)]


<220>
<221>  misc_feature
<222>  (1)..(6432)
<223>  Vector pET-28b{Ptac}[hGLYAT2(co_Ec)]

<400>  15
ggccgctctg gtggatccaa ttgtgagcgg ataacaatta cgagcttcat gcacagtgat       60

cgacgctgtt gacaattaat catcggctcg tataatgtgt ggatgtggaa ttgtgagcgc      120

tcacaattcc acaacggttt ccctctagaa ataattttgt ttaacaggag gtaaaacata      180

tgctggttct gcacaacagc caaaaactgc aaattctgta caaatctctg gaaaaatcta      240

ttcctgaaag cattaaagtt tatggtgcga tcttcaacat caaggataaa aaccctttca      300

atatggaggt gctggtcgac gcgtggcctg attaccaaat cgtcatcacc cgtccgcaga      360

agcaagaaat gaaagatgac caggaccact atactaacac ctaccacatc ttcactaaag      420

cgccggacaa actggaagaa gttctgagct actccaatgt tatctcctgg gaacaaacgc      480

tgcagattca aggttgccag gaaggtctgg atgaggcgat tcgtaaggtc gctacctcta      540

agtccgttca agtcgattac atgaaaacca tcctgtttat cccggagctg ccgaagaaac      600

acaaaacctc ctctaacgac aagatggagc tgttcgaagt agacgatgac aacaaagagg      660

gtaacttttc caatatgttc ctggacgcct cccacgccgg tctggttaat gagcactggg      720

cgttcggtaa aaacgaacgc tccctgaagt acattgagcg ttgtctgcag gattttctgg      780

gttttggtgt cctgggtcca gagggtcaac tggtctcttg gatcgttatg gaacagtctt      840

gtgaactgcg tatgggttat actgtgccaa aataccgtca ccaaggtaat atgctgcaaa      900

tcggttatca tctggagaag tacctgagcc agaaggaaat cccgttctat ttccatgttg      960

ccgataataa cgaaaagagc ctgcaagccc tgaacaatct gggcttcaag atctgccctt     1020

gcggttggca ccagtggaag tgcacgccta aaaagtactg ttaagcgtta agtctgagag     1080

gtgagagctc gaattcggat cccgacccat ttgctgtcca ccagtcatgc tagccatatg     1140

gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt     1200

atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca     1260

attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg     1320

acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga     1380

catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt     1440

gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc     1500

attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca     1560

ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc     1620

gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag     1680

taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg     1740

tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg     1800

agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga     1860

ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta     1920

aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg     1980

tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca     2040

ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc     2100

cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg     2160

aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc     2220

tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat     2280

atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt     2340

ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg     2400

ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg     2460

ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc     2520

cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct     2580

tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg     2640

tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg     2700

attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac     2760

gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga     2820

gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt     2880

atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt     2940

ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc     3000

ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc     3060

gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg     3120

tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg     3180

atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca     3240

tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc     3300

accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca     3360

tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc     3420

cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta     3480

acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat     3540

cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc     3600

tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa     3660

caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc     3720

gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca     3780

gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt     3840

ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc     3900

ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat     3960

accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac     4020

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     4080

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     4140

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     4200

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     4260

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     4320

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     4380

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     4440

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     4500

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     4560

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     4620

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     4680

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     4740

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     4800

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca ataaaactgt     4860

ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt     4920

gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc     4980

gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc     5040

cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg     5100

tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta     5160

ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat     5220

tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc     5280

ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg     5340

ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc     5400

gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac     5460

cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga     5520

aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg     5580

ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa     5640

aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt     5700

ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa aaataaacaa     5760

ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt     5820

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     5880

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     5940

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     6000

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     6060

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     6120

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     6180

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     6240

ccgctacagg gcgcgtccca ttcgccatcc ggatatagtt cctcctttca gcaaaaaacc     6300

cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag cggtggcagc     6360

agccaactca gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg     6420

gtgctcgagt gc                                                         6432


<210>  16
<211>  6418
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET-28b{Ptac}[hGLYAT3(co_Ec)]


<220>
<221>  misc_feature
<222>  (1)..(6418)
<223>  pET-28b{Ptac}[hGLYAT3(co_Ec)]

<400>  16
ggccgccctt cattttggat ccaattgtga gcggataaca attacgagct tcatgcacag       60

tgatcgacgc tgttgacaat taatcatcgg ctcgtataat gtgtggatgt ggaattgtga      120

gcgctcacaa ttccacaacg gtttccctct agaaataatt ttgtttaaca ggaggtaaaa      180

catatgctgg ttctgaattg ctctactaaa ctgctgatcc tggagaaaat gctgaaatcc      240

tgtttcccgg aatctctgaa agtttatggt gcggtaatga atatcaaccg tggtaatccg      300

tttcaaaaag aagttgttct ggactcttgg ccagatttca aggcggttat cacccgtcgt      360

cagcgtgaag cggaaaccga caacctggac cactatacca acgcgtatgc ggttttctat      420

aaagatgttc gcgcgtaccg tcagctgctg gaagaatgcg atgttttcaa ctgggaccag      480

gtttttcaga tccaaggtct gcaatccgag ctgtacgacg tttctaaggc cgtggcgaac      540

tctaaacaac tgaacatcaa gctgacctct ttcaaagcgg ttcatttctc tccggtatcc      600

agcctgccgg acacctcctt cctgaaaggt ccgtctccgc gtctgaccta cctgtctgtt      660

gcgaatgcgg atctgctgaa ccgtacttgg tcccgtggtg gcaacgaaca gtgcctgcgt      720

tacattgcta acctgatctc ttgcttcccg agcgtctgcg ttcgtgatga aaaaggtaac      780

ccagtatctt ggtctatcac cgaccagttc gcgaccatgt gtcatggcta caccctgcct      840

gaacaccgtc gtaagggtta ctctcgtctg gttgcgctga ccctggcgcg taagctgcag      900

tctcgtggtt ttccgtccca gggtaacgtc ctggacgaca acactgcgtc catctccctg      960

ctgaagtccc tgcatgccga attcctgccg tgccgtttcc accgtctgat tctgacccca     1020

gcgaccttct ctggtctgcc gcacctgtaa gcgttaagtc tgagaggtga gagctcgaat     1080

tcggatcccg acccatttgc tgtccaccag tcatgctagc catatggctg ccgcgcggca     1140

ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta     1200

aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg     1260

agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg     1320

gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg     1380

aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag     1440

gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg     1500

cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg     1560

gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga     1620

tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat     1680

gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc     1740

cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt     1800

cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc     1860

tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat     1920

caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa     1980

gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg     2040

gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt     2100

gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga     2160

ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca     2220

ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat     2280

caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa     2340

accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag     2400

atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc     2460

tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc     2520

atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct     2580

cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc     2640

accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag     2700

ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag     2760

ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt     2820

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     2880

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     2940

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     3000

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     3060

tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg     3120

gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac     3180

gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg     3240

tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg     3300

gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa     3360

gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg     3420

tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag     3480

catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga     3540

ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca     3600

gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg     3660

tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga     3720

tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc     3780

ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg     3840

cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca     3900

tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg     3960

taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct     4020

cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca     4080

cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga     4140

accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     4200

acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     4260

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     4320

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     4380

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     4440

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     4500

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     4560

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg     4620

gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg     4680

gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca     4740

gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga     4800

acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac     4860

agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga     4920

ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg     4980

caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg     5040

aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg     5100

ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca     5160

tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct     5220

gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt     5280

cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca     5340

cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct     5400

gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc     5460

actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt     5520

attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac     5580

tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat     5640

aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta     5700

attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     5760

cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc     5820

gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc     5880

ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag     5940

tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga     6000

tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc     6060

actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa     6120

cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt     6180

agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc     6240

gtcccattcg ccatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt     6300

tagaggcccc aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt     6360

cctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgc       6418


<210>  17
<211>  4334
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(4334)
<223>  pBMT-3_ccdAB

<400>  17
ctgatggaca ggctgcgcct gcccacgagc ttgaccacag ggattgccca ccggctaccc       60

agccttcgac cacataccca ccggctccaa ctgcgcggcc tgcggccttg ccccatcaat      120

ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc ctgcgcgctt cgcttgccgg      180

ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg accgcgcagc ggcttggcct      240

tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc agtcgaaggc gaagcccgcc      300

cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt ccaagggggc agcgccacct      360

tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc ggaggggcca ctttttgccg      420

gagggggagc cgcgccgaag gcgtggggga accccgcagg ggtgcccttc tttgggcacc      480

aaagaactag atatagggcg aaatgcgaaa gacttaaaaa tcaacaactt aaaaaagggg      540

ggtacgcaac agctcattgc ggcacccccc gcaatagctc attgcgtagg ttaaagaaaa      600

tctgtaattg actgccactt ttacgcaacg cataattgtt gtcgcgctgc cgaaaagttg      660

cagctgattg cgcatggtgc cgcaaccgtg cggcacccta ccgcatggag ataagcatgg      720

ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa gcccggtcac tgggtgcaaa      780

cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc gaggaaaccc acggcggcaa      840

tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa cgccgtggtg gtcagccaga      900

agacactttc caagctcatc ggacgttctt tgcggacggt ccaatacgca gtcaaggact      960

tggtggccga gcgctggatc tccgtcgtga agctcaacgg ccccggcacc gtgtcggcct     1020

acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga ccagttgcgc ctgtcggtgt     1080

tcagtgccgc cgtggtggtt gatcacgacg accaggacga atcgctgttg gggcatggcg     1140

acctgcgccg catcccgacc ctgtatccgg gcgagcagca actaccgacc ggccccggcg     1200

aggagccgcc cagccagccc ggcattccgg gcatggaacc agacctgcca gccttgaccg     1260

aaacggagga atgggaacgg cgcgggcagc agcgcctgcc gatgcccgat gagccgtgtt     1320

ttctggacga tggcgagccg ttggagccgc cgacacgggt cacgctgccg cgccggtagt     1380

acgtacccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta     1440

aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa     1500

gagacaggat gaggatcgtt tcgatgaagc agcgtattac agtgacagtt gacagcgaca     1560

gctatcagtt gctcaaggca tatgatgtca atatctccgg tctggtaagc acaaccatgc     1620

agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg     1680

ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt     1740

gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat     1800

gtacagagcg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca     1860

cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa     1920

agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa     1980

gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg     2040

ggaatataaa tcctagacga attctctagt agaggttcca actttcacca taatgaaata     2100

agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa     2160

atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa     2220

cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat     2280

attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt     2340

cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt     2400

gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa     2460

acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat     2520

tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag     2580

aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg     2640

gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc     2700

gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat     2760

gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa     2820

agatctggat ccccctcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg     2880

gaggtcaggt atgatttaaa tggtcagtat tgagcgatat ctagagaatt cgtcaacgaa     2940

ttcaagcttg atatcattca ggacgagcct cagactccag cgtaactgga ctgaaaacaa     3000

actaaagcgc ccttgtggcg ctttagtttt gttccgctca tgataataat ggtttcttag     3060

acgtcaggtg gcacttttcg gggaaatgtg cgcgcccgcg ttcctgctgg cgctgggcct     3120

gtttctggcg ctggacttcc cgctgttccg tcagcagctt ttcgcccacg gccttgatga     3180

tcgcggcggc cttggcctgc atatcccgat tcaacggccc cagggcgtcc agaacgggct     3240

tcaggcgctc ccgaaggtct cgggccgtct cttgggcttg atcggccttc ttgcgcatct     3300

cacgcgctcc tgcggcggcc tgtagggcag gctcataccc ctgccgaacc gcttttgtca     3360

gccggtcggc cacggcttcc ggcgtctcaa cgcgctttga gattcccagc ttttcggcca     3420

atccctgcgg tgcataggcg cgtggctcga ccgcttgcgg gctgatggtg acgtggccca     3480

ctggtggccg ctccagggcc tcgtagaacg cctgaatgcg cgtgtgacgt gccttgctgc     3540

cctcgatgcc ccgttgcagc cctagatcgg ccacagcggc cgcaaacgtg gtctggtcgc     3600

gggtcatctg cgctttgttg ccgatgaact ccttggccga cagcctgccg tcctgcgtca     3660

gcggcaccac gaacgcggtc atgtgcgggc tggtttcgtc acggtggatg ctggccgtca     3720

cgatgcgatc cgccccgtac ttgtccgcca gccacttgtg cgccttctcg aagaacgccg     3780

cctgctgttc ttggctggcc gacttccacc attccgggct ggccgtcatg acgtactcga     3840

ccgccaacac agcgtccttg cgccgcttct ctggcagcaa ctcgcgcagt cggcccatcg     3900

cttcatcggt gctgctggcc gcccagtgct cgttctctgg cgtcctgctg gcgtcagcgt     3960

tgggcgtctc gcgctcgcgg taggcgtgct tgagactggc cgccacgttg cccattttcg     4020

ccagcttctt gcatcgcatg atcgcgtatg ccgccatgcc tgcccctccc ttttggtgtc     4080

caaccggctc gacgggggca gcgcaaggcg gtgcctccgg cgggccactc aatgcttgag     4140

tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta     4200

cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga     4260

tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa     4320

ccctgctgga caag                                                       4334


<210>  18
<211>  5024
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(5024)
<223>  pBMT-3_ccdAB_PT7-'tesA

<400>  18
caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctagtt attgctcagc       60

ggtggcagca gcctaggtta attaagctgc gctagtagac gagtccatgt gctggcgttc      120

aaatttcgca gcagcggttt ctttaccaga ctcgaaaatt caggagtcgt ggttgaccag      180

cggctgcagt tgcttggcca tccagtcggc gatgaacggc tgcgcgtcgc ggttcgggtg      240

gatgccgtcg tcctgcatcc actgcggctt caggtagact tcctccatga agaacggcag      300

cagcggcacg tcgaattcct tggccagctt cgggtagatc gcggagaagg cctcgttgta      360

gcgacggccg tagttcgccg gcaggcggat ctgcatcagc agcggctcgg cgttcgcggc      420

cttcacgtcc tgcaggatct ggcgcagcgt ctgttcggtc tgctgcggct ggaagccgcg      480

caggccgtcg ttgccgccca gctcgaccag cacccagcgc ggctggtgct gcttcagcag      540

cgccggcagg cgggccaggc cctgctggct cgtgtcgccg ctgatcgagg cgttgaccac      600

cgaggtcttg gactgccact tgtcgttcag cagcgccggc cacgccgccg acgccgacat      660

gcggtagccc gccgacaggg agtcgcccag gatcagcagg gtgtccgccg ccatggtata      720

tctccttatt aaagttaaac aaaattattt ctacagggga attgttatcc gctcacaatt      780

cccctatagt gagtcgtatt aatttcctaa tgcaggagtc gatcattcag gacgagcctc      840

agactccagc gtaactggac tgaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg      900

ttccgctcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc      960

gcgcccgcgt tcctgctggc gctgggcctg tttctggcgc tggacttccc gctgttccgt     1020

cagcagcttt tcgcccacgg ccttgatgat cgcggcggcc ttggcctgca tatcccgatt     1080

caacggcccc agggcgtcca gaacgggctt caggcgctcc cgaaggtctc gggccgtctc     1140

ttgggcttga tcggccttct tgcgcatctc acgcgctcct gcggcggcct gtagggcagg     1200

ctcatacccc tgccgaaccg cttttgtcag ccggtcggcc acggcttccg gcgtctcaac     1260

gcgctttgag attcccagct tttcggccaa tccctgcggt gcataggcgc gtggctcgac     1320

cgcttgcggg ctgatggtga cgtggcccac tggtggccgc tccagggcct cgtagaacgc     1380

ctgaatgcgc gtgtgacgtg ccttgctgcc ctcgatgccc cgttgcagcc ctagatcggc     1440

cacagcggcc gcaaacgtgg tctggtcgcg ggtcatctgc gctttgttgc cgatgaactc     1500

cttggccgac agcctgccgt cctgcgtcag cggcaccacg aacgcggtca tgtgcgggct     1560

ggtttcgtca cggtggatgc tggccgtcac gatgcgatcc gccccgtact tgtccgccag     1620

ccacttgtgc gccttctcga agaacgccgc ctgctgttct tggctggccg acttccacca     1680

ttccgggctg gccgtcatga cgtactcgac cgccaacaca gcgtccttgc gccgcttctc     1740

tggcagcaac tcgcgcagtc ggcccatcgc ttcatcggtg ctgctggccg cccagtgctc     1800

gttctctggc gtcctgctgg cgtcagcgtt gggcgtctcg cgctcgcggt aggcgtgctt     1860

gagactggcc gccacgttgc ccattttcgc cagcttcttg catcgcatga tcgcgtatgc     1920

cgccatgcct gcccctccct tttggtgtcc aaccggctcg acgggggcag cgcaaggcgg     1980

tgcctccggc gggccactca atgcttgagt atactcacta gactttgctt cgcaaagtcg     2040

tgaccgccta cggcggctgc ggcgccctac gggcttgctc tccgggcttc gccctgcgcg     2100

gtcgctgcgc tcccttgcca gcccgtggat atgtggacga tggccgcgag cggccaccgg     2160

ctggctcgct tcgctcggcc cgtggacaac cctgctggac aagctgatgg acaggctgcg     2220

cctgcccacg agcttgacca cagggattgc ccaccggcta cccagccttc gaccacatac     2280

ccaccggctc caactgcgcg gcctgcggcc ttgccccatc aattttttta attttctctg     2340

gggaaaagcc tccggcctgc ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa     2400

ggcgggtcaa ggctcgcgca gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga     2460

cccaagccta tgcgagtggg ggcagtcgaa ggcgaagccc gcccgcctgc cccccgagcc     2520

tcacggcggc gagtgcgggg gttccaaggg ggcagcgcca ccttgggcaa ggccgaaggc     2580

cgcgcagtcg atcaacaagc cccggagggg ccactttttg ccggaggggg agccgcgccg     2640

aaggcgtggg ggaaccccgc aggggtgccc ttctttgggc accaaagaac tagatatagg     2700

gcgaaatgcg aaagacttaa aaatcaacaa cttaaaaaag gggggtacgc aacagctcat     2760

tgcggcaccc cccgcaatag ctcattgcgt aggttaaaga aaatctgtaa ttgactgcca     2820

cttttacgca acgcataatt gttgtcgcgc tgccgaaaag ttgcagctga ttgcgcatgg     2880

tgccgcaacc gtgcggcacc ctaccgcatg gagataagca tggccacgca gtccagagaa     2940

atcggcattc aagccaagaa caagcccggt cactgggtgc aaacggaacg caaagcgcat     3000

gaggcgtggg ccgggcttat tgcgaggaaa cccacggcgg caatgctgct gcatcacctc     3060

gtggcgcaga tgggccacca gaacgccgtg gtggtcagcc agaagacact ttccaagctc     3120

atcggacgtt ctttgcggac ggtccaatac gcagtcaagg acttggtggc cgagcgctgg     3180

atctccgtcg tgaagctcaa cggccccggc accgtgtcgg cctacgtggt caatgaccgc     3240

gtggcgtggg gccagccccg cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg     3300

gttgatcacg acgaccagga cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg     3360

accctgtatc cgggcgagca gcaactaccg accggccccg gcgaggagcc gcccagccag     3420

cccggcattc cgggcatgga accagacctg ccagccttga ccgaaacgga ggaatgggaa     3480

cggcgcgggc agcagcgcct gccgatgccc gatgagccgt gttttctgga cgatggcgag     3540

ccgttggagc cgccgacacg ggtcacgctg ccgcgccggt agtacgtacc cggaattgcc     3600

agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt     3660

gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc     3720

gtttcgatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag     3780

gcatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt     3840

ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt     3900

attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt     3960

ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gcgatattat     4020

tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa     4080

agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac     4140

caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca     4200

ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatcctaga     4260

cgaattctct agtagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt     4320

attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac     4380

tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca     4440

gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa     4500

gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct     4560

gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga     4620

tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg     4680

gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg     4740

ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc     4800

agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt     4860

cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc     4920

gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa     4980

tgaattacaa cagtactgcg atgagtggca gggcggggcg taaa                      5024


<210>  19
<211>  8268
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(8268)
<223>  pBMT-3_ccdAB_PT7-nphT7-hbd-crt-ter

<400>  19
ccttgccagc ccgtggatat gtggacgatg gccgcgagcg gccaccggct ggctcgcttc       60

gctcggcccg tggacaaccc tgctggacaa gctgatggac aggctgcgcc tgcccacgag      120

cttgaccaca gggattgccc accggctacc cagccttcga ccacataccc accggctcca      180

actgcgcggc ctgcggcctt gccccatcaa tttttttaat tttctctggg gaaaagcctc      240

cggcctgcgg cctgcgcgct tcgcttgccg gttggacacc aagtggaagg cgggtcaagg      300

ctcgcgcagc gaccgcgcag cggcttggcc ttgacgcgcc tggaacgacc caagcctatg      360

cgagtggggg cagtcgaagg cgaagcccgc ccgcctgccc cccgagcctc acggcggcga      420

gtgcgggggt tccaaggggg cagcgccacc ttgggcaagg ccgaaggccg cgcagtcgat      480

caacaagccc cggaggggcc actttttgcc ggagggggag ccgcgccgaa ggcgtggggg      540

aaccccgcag gggtgccctt ctttgggcac caaagaacta gatatagggc gaaatgcgaa      600

agacttaaaa atcaacaact taaaaaaggg gggtacgcaa cagctcattg cggcaccccc      660

cgcaatagct cattgcgtag gttaaagaaa atctgtaatt gactgccact tttacgcaac      720

gcataattgt tgtcgcgctg ccgaaaagtt gcagctgatt gcgcatggtg ccgcaaccgt      780

gcggcaccct accgcatgga gataagcatg gccacgcagt ccagagaaat cggcattcaa      840

gccaagaaca agcccggtca ctgggtgcaa acggaacgca aagcgcatga ggcgtgggcc      900

gggcttattg cgaggaaacc cacggcggca atgctgctgc atcacctcgt ggcgcagatg      960

ggccaccaga acgccgtggt ggtcagccag aagacacttt ccaagctcat cggacgttct     1020

ttgcggacgg tccaatacgc agtcaaggac ttggtggccg agcgctggat ctccgtcgtg     1080

aagctcaacg gccccggcac cgtgtcggcc tacgtggtca atgaccgcgt ggcgtggggc     1140

cagccccgcg accagttgcg cctgtcggtg ttcagtgccg ccgtggtggt tgatcacgac     1200

gaccaggacg aatcgctgtt ggggcatggc gacctgcgcc gcatcccgac cctgtatccg     1260

ggcgagcagc aactaccgac cggccccggc gaggagccgc ccagccagcc cggcattccg     1320

ggcatggaac cagacctgcc agccttgacc gaaacggagg aatgggaacg gcgcgggcag     1380

cagcgcctgc cgatgcccga tgagccgtgt tttctggacg atggcgagcc gttggagccg     1440

ccgacacggg tcacgctgcc gcgccggtag tacgtacccg gaattgccag ctggggcgcc     1500

ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat     1560

ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgatgaag     1620

cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatgatgtc     1680

aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa     1740

cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac     1800

ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa     1860

aagagagagc cgttatcgtc tgtttgtgga tgtacagagc gatattattg acacgcccgg     1920

gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga     1980

actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc     2040

cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga     2100

catcaaaaac gccattaacc tgatgttctg gggaatataa atcctagacg aattctctag     2160

tagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt     2220

atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac     2280

cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca     2340

atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa     2400

aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca     2460

tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc     2520

ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca     2580

cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa     2640

cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg     2700

ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt     2760

tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca     2820

ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca     2880

gtactgcgat gagtggcagg gcggggcgta aaaaatccct tatgcgactc ctgcattagg     2940

aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tgtagaaata     3000

attttgttta actttaataa ggagatatac catgacggat gtccgctttc gtatcattgg     3060

cacgggcgct tacgtcccgg aacgcattgt cagcaacgac gaagtcggcg caccggctgg     3120

tgtggatgac gattggatta cgcgtaaaac cggtatccgc cagcgtcgct gggcggccga     3180

cgatcaagca acgagcgatc tggctaccgc agctggtcgt gcagcactga aagcagctgg     3240

cattacgccg gaacagctga ccgtcatcgc agtggctacc tctacgccgg accgtccgca     3300

gccgccgacc gcggcctatg ttcaacatca cctgggtgca accggcacgg cagcttttga     3360

tgttaacgct gtgtgcagcg gtacggtttt cgcgctgagc tctgttgccg gcaccctggt     3420

ctatcgtggc ggttacgcac tggtcattgg tgctgatctg tactcacgta tcctgaatcc     3480

ggcggaccgc aaaaccgtgg ttctgttcgg cgatggtgcg ggcgcgatgg tgctgggccc     3540

gaccagcacc ggcaccggtc cgattgtgcg tcgcgttgca ctgcatacgt ttggcggtct     3600

gaccgatctg atccgtgttc cggccggcgg ttcccgccag ccgctggaca ccgatggtct     3660

ggacgcaggc ctgcaatatt ttgcgatgga tggccgcgaa gtccgtcgct tcgtgaccga     3720

acatctgccg cagctgatta aaggttttct gcacgaagcg ggcgtggatg cggcggatat     3780

tagccatttc gtgccgcacc aagccaacgg tgtgatgctg gacgaagttt ttggcgaact     3840

gcatctgccg cgtgcaacca tgcaccgtac ggtggaaacc tacggtaata cgggcgcagc     3900

tagtattccg atcacgatgg atgcggccgt tcgtgcaggt tccttccgtc cgggcgaact     3960

ggttctgctg gcgggctttg gcggcggtat ggcggcttcg tttgctctga ttgaatggtg     4020

acctaatgca ggctgcaggc ggatacgagg aggaataaac catgaaaaag gtatgtgtta     4080

taggtgcagg tactatgggt tcaggaattg ctcaggcatt tgcagctaaa ggatttgaag     4140

tagtattaag agatattaaa gatgaatttg ttgatagagg attagatttt atcaataaaa     4200

atctttctaa attagttaaa aaaggaaaga tagaagaagc tactaaagtt gaaatcttaa     4260

ctagaatttc cggaacagtt gaccttaata tggcagctga ttgcgattta gttatagaag     4320

cagctgttga aagaatggat attaaaaagc agatttttgc tgacttagac aatatatgca     4380

agccagaaac aattcttgca tcaaatacat catcactttc aataacagaa gtggcatcag     4440

caactaaaag acctgataag gttataggta tgcatttctt taatccagct cctgttatga     4500

agcttgtaga ggtaataaga ggaatagcta catcacaaga aacttttgat gcagttaaag     4560

agacatctat agcaatagga aaagatcctg tagaagtagc agaagcacca ggatttgttg     4620

taaatagaat attaatacca atgattaatg aagcagttgg tatattagca gaaggaatag     4680

cttcagtaga agacatagat aaagctatga aacttggagc taatcaccca atgggaccat     4740

tagaattagg tgattttata ggtcttgata tatgtcttgc tataatggat gttttatact     4800

cagaaactgg agattctaag tatagaccac atacattact taagaagtat gtaagagcag     4860

gatggcttgg aagaaaatca ggaaaaggtt tctacgatta ttcaaaataa gtttacagga     4920

tctgcaggga ggaggaaatc atggagttga acaacgttat tctggagaaa gaaggcaagg     4980

tggcggttgt caccattaac cgtccaaagg ccctgaacgc tctgaactcg gataccctga     5040

aagagatgga ttacgttatt ggcgagattg agaatgacag cgaagtgctg gctgtgattc     5100

tgaccggtgc gggtgagaag agctttgtcg cgggtgcgga catcagcgag atgaaagaaa     5160

tgaacaccat cgaaggccgt aagttcggta ttctgggcaa caaggtgttt cgtcgtctgg     5220

aactgctgga gaaacctgtc attgctgccg tgaacggttt cgcgctgggc ggtggttgcg     5280

agatcgctat gagctgcgat attcgtatcg catcgtccaa cgcacgcttt ggtcaaccgg     5340

aggtcggtct gggtatcact ccgggtttcg gcggtacgca acgtctgagc cgcctggttg     5400

gcatgggcat ggcgaaacag ttgattttca cggcacagaa cattaaggcg gatgaggcgc     5460

tgcgtattgg tctggtgaat aaggtcgttg agccaagcga actgatgaat accgcgaaag     5520

aaattgcgaa caagatcgtt agcaatgccc cggtggccgt taagctgtcg aaacaggcaa     5580

tcaaccgtgg catgcagtgt gacatcgaca ccgccctggc gtttgagagc gaggcgtttg     5640

gtgagtgctt ctccaccgag gaccaaaagg atgcgatgac cgcgttcatt gagaaacgca     5700

agatcgaggg tttcaagaat cgttaataga ggaggatagg aggttttcat atgattgtga     5760

aaccgatggt ccgtaataat atctgtctga atgctcaccc gcagggctgt aaaaaaggcg     5820

tggaagatca aattgaatat accaaaaaac gtattacggc agaagtgaaa gccggcgcaa     5880

aagctccgaa aaacgtgctg gttctgggtt gcagcaatgg ctatggtctg gcttctcgca     5940

ttaccgcggc ctttggctac ggtgcagcta cgatcggcgt tagtttcgaa aaagcaggtt     6000

ccgaaaccaa atatggcacg ccgggttggt acaacaatct ggcttttgat gaagcggcca     6060

aacgtgaagg cctgtatagt gtcaccattg atggtgacgc gttctccgat gaaattaaag     6120

cacaggtgat cgaagaagcg aagaaaaaag gcattaaatt tgacctgatc gtttacagcc     6180

tggcatctcc ggtccgtacc gatccggaca cgggtatcat gcataaatct gtgctgaaac     6240

cgtttggcaa aaccttcacg ggtaaaaccg ttgatccgtt cacgggcgaa ctgaaagaaa     6300

ttagcgcgga accggccaac gatgaagaag cagctgcgac cgtcaaagtg atgggcggtg     6360

aagactggga acgttggatc aaacagctga gtaaagaagg cctgctggaa gaaggttgca     6420

ttaccctggc gtattcctac atcggcccgg aagcaaccca agctctgtat cgcaaaggca     6480

cgattggtaa agcgaaagaa catctggaag cgaccgccca ccgtctgaac aaagaaaatc     6540

cgtcaatccg cgccttcgtt tcggtcaata aaggtctggt tacccgtgca tcagctgtga     6600

ttccggttat cccgctgtac ctggcatcgc tgtttaaagt catgaaagaa aaaggcaacc     6660

atgaaggttg tattgaacag atcacccgcc tgtatgccga acgtctgtac cgcaaagatg     6720

gtacgattcc ggtggacgaa gaaaatcgta ttcgcatcga tgactgggaa ctggaagaag     6780

atgtccaaaa agccgtgagc gccctgatgg aaaaagttac cggcgaaaac gcggaatctc     6840

tgacggatct ggccggttat cgtcacgact ttctggcgag taatggtttt gatgttgaag     6900

gcattaacta cgaagctgaa gtggaacgct ttgatcgcat ttgatctaga gaattcgtca     6960

acgaattcaa gcttgatatc attcaggacg agcctcagac tccagcgtaa ctggactgaa     7020

aacaaactaa agcgcccttg tggcgcttta gttttgttcc gctcatgata ataatggttt     7080

cttagacgtc aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg     7140

ggcctgtttc tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt     7200

gatgatcgcg gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac     7260

gggcttcagg cgctcccgaa ggtctcgggc cgtctcttgg gcttgatcgg ccttcttgcg     7320

catctcacgc gctcctgcgg cggcctgtag ggcaggctca tacccctgcc gaaccgcttt     7380

tgtcagccgg tcggccacgg cttccggcgt ctcaacgcgc tttgagattc ccagcttttc     7440

ggccaatccc tgcggtgcat aggcgcgtgg ctcgaccgct tgcgggctga tggtgacgtg     7500

gcccactggt ggccgctcca gggcctcgta gaacgcctga atgcgcgtgt gacgtgcctt     7560

gctgccctcg atgccccgtt gcagccctag atcggccaca gcggccgcaa acgtggtctg     7620

gtcgcgggtc atctgcgctt tgttgccgat gaactccttg gccgacagcc tgccgtcctg     7680

cgtcagcggc accacgaacg cggtcatgtg cgggctggtt tcgtcacggt ggatgctggc     7740

cgtcacgatg cgatccgccc cgtacttgtc cgccagccac ttgtgcgcct tctcgaagaa     7800

cgccgcctgc tgttcttggc tggccgactt ccaccattcc gggctggccg tcatgacgta     7860

ctcgaccgcc aacacagcgt ccttgcgccg cttctctggc agcaactcgc gcagtcggcc     7920

catcgcttca tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc tgctggcgtc     7980

agcgttgggc gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca cgttgcccat     8040

tttcgccagc ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc ctcccttttg     8100

gtgtccaacc ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc cactcaatgc     8160

ttgagtatac tcactagact ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg     8220

ccctacgggc ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctc                  8268


<210>  20
<211>  8959
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(8959)
<223>  pBMT-3_ccdAB_PT7-?tesA_PT7-nphT7-hbd-crt-ter

<400>  20
ccttgccagc ccgtggatat gtggacgatg gccgcgagcg gccaccggct ggctcgcttc       60

gctcggcccg tggacaaccc tgctggacaa gctgatggac aggctgcgcc tgcccacgag      120

cttgaccaca gggattgccc accggctacc cagccttcga ccacataccc accggctcca      180

actgcgcggc ctgcggcctt gccccatcaa tttttttaat tttctctggg gaaaagcctc      240

cggcctgcgg cctgcgcgct tcgcttgccg gttggacacc aagtggaagg cgggtcaagg      300

ctcgcgcagc gaccgcgcag cggcttggcc ttgacgcgcc tggaacgacc caagcctatg      360

cgagtggggg cagtcgaagg cgaagcccgc ccgcctgccc cccgagcctc acggcggcga      420

gtgcgggggt tccaaggggg cagcgccacc ttgggcaagg ccgaaggccg cgcagtcgat      480

caacaagccc cggaggggcc actttttgcc ggagggggag ccgcgccgaa ggcgtggggg      540

aaccccgcag gggtgccctt ctttgggcac caaagaacta gatatagggc gaaatgcgaa      600

agacttaaaa atcaacaact taaaaaaggg gggtacgcaa cagctcattg cggcaccccc      660

cgcaatagct cattgcgtag gttaaagaaa atctgtaatt gactgccact tttacgcaac      720

gcataattgt tgtcgcgctg ccgaaaagtt gcagctgatt gcgcatggtg ccgcaaccgt      780

gcggcaccct accgcatgga gataagcatg gccacgcagt ccagagaaat cggcattcaa      840

gccaagaaca agcccggtca ctgggtgcaa acggaacgca aagcgcatga ggcgtgggcc      900

gggcttattg cgaggaaacc cacggcggca atgctgctgc atcacctcgt ggcgcagatg      960

ggccaccaga acgccgtggt ggtcagccag aagacacttt ccaagctcat cggacgttct     1020

ttgcggacgg tccaatacgc agtcaaggac ttggtggccg agcgctggat ctccgtcgtg     1080

aagctcaacg gccccggcac cgtgtcggcc tacgtggtca atgaccgcgt ggcgtggggc     1140

cagccccgcg accagttgcg cctgtcggtg ttcagtgccg ccgtggtggt tgatcacgac     1200

gaccaggacg aatcgctgtt ggggcatggc gacctgcgcc gcatcccgac cctgtatccg     1260

ggcgagcagc aactaccgac cggccccggc gaggagccgc ccagccagcc cggcattccg     1320

ggcatggaac cagacctgcc agccttgacc gaaacggagg aatgggaacg gcgcgggcag     1380

cagcgcctgc cgatgcccga tgagccgtgt tttctggacg atggcgagcc gttggagccg     1440

ccgacacggg tcacgctgcc gcgccggtag tacgtacccg gaattgccag ctggggcgcc     1500

ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat     1560

ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgatgaag     1620

cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatgatgtc     1680

aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa     1740

cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac     1800

ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa     1860

aagagagagc cgttatcgtc tgtttgtgga tgtacagagc gatattattg acacgcccgg     1920

gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga     1980

actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc     2040

cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga     2100

catcaaaaac gccattaacc tgatgttctg gggaatataa atcctagacg aattctctag     2160

tagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt     2220

atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac     2280

cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca     2340

atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa     2400

aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca     2460

tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc     2520

ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca     2580

cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa     2640

cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg     2700

ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt     2760

tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca     2820

ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca     2880

gtactgcgat gagtggcagg gcggggcgta aagatcttct cgacgctctc ccttatgcga     2940

ctcctgcatt aggaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc     3000

ccctgtagaa ataattttgt ttaactttaa taaggagata taccatggcg gcggacaccc     3060

tgctgatcct gggcgactcc ctgtcggcgg gctaccgcat gtcggcgtcg gcggcgtggc     3120

cggcgctgct gaacgacaag tggcagtcca agacctcggt ggtcaacgcc tcgatcagcg     3180

gcgacacgag ccagcagggc ctggcccgcc tgccggcgct gctgaagcag caccagccgc     3240

gctgggtgct ggtcgagctg ggcggcaacg acggcctgcg cggcttccag ccgcagcaga     3300

ccgaacagac gctgcgccag atcctgcagg acgtgaaggc cgcgaacgcc gagccgctgc     3360

tgatgcagat ccgcctgccg gcgaactacg gccgtcgcta caacgaggcc ttctccgcga     3420

tctacccgaa gctggccaag gaattcgacg tgccgctgct gccgttcttc atggaggaag     3480

tctacctgaa gccgcagtgg atgcaggacg acggcatcca cccgaaccgc gacgcgcagc     3540

cgttcatcgc cgactggatg gccaagcaac tgcagccgct ggtcaaccac gactcctgaa     3600

tttaaatccc ttatgcgact cctgcattag gaaattaata cgactcacta taggggaatt     3660

gtgagcggat aacaattccc ctgtagaaat aattttgttt aactttaata aggagatata     3720

ccatgacgga tgtccgcttt cgtatcattg gcacgggcgc ttacgtcccg gaacgcattg     3780

tcagcaacga cgaagtcggc gcaccggctg gtgtggatga cgattggatt acgcgtaaaa     3840

ccggtatccg ccagcgtcgc tgggcggccg acgatcaagc aacgagcgat ctggctaccg     3900

cagctggtcg tgcagcactg aaagcagctg gcattacgcc ggaacagctg accgtcatcg     3960

cagtggctac ctctacgccg gaccgtccgc agccgccgac cgcggcctat gttcaacatc     4020

acctgggtgc aaccggcacg gcagcttttg atgttaacgc tgtgtgcagc ggtacggttt     4080

tcgcgctgag ctctgttgcc ggcaccctgg tctatcgtgg cggttacgca ctggtcattg     4140

gtgctgatct gtactcacgt atcctgaatc cggcggaccg caaaaccgtg gttctgttcg     4200

gcgatggtgc gggcgcgatg gtgctgggcc cgaccagcac cggcaccggt ccgattgtgc     4260

gtcgcgttgc actgcatacg tttggcggtc tgaccgatct gatccgtgtt ccggccggcg     4320

gttcccgcca gccgctggac accgatggtc tggacgcagg cctgcaatat tttgcgatgg     4380

atggccgcga agtccgtcgc ttcgtgaccg aacatctgcc gcagctgatt aaaggttttc     4440

tgcacgaagc gggcgtggat gcggcggata ttagccattt cgtgccgcac caagccaacg     4500

gtgtgatgct ggacgaagtt tttggcgaac tgcatctgcc gcgtgcaacc atgcaccgta     4560

cggtggaaac ctacggtaat acgggcgcag ctagtattcc gatcacgatg gatgcggccg     4620

ttcgtgcagg ttccttccgt ccgggcgaac tggttctgct ggcgggcttt ggcggcggta     4680

tggcggcttc gtttgctctg attgaatggt gacctaatgc aggctgcagg cggatacgag     4740

gaggaataaa ccatgaaaaa ggtatgtgtt ataggtgcag gtactatggg ttcaggaatt     4800

gctcaggcat ttgcagctaa aggatttgaa gtagtattaa gagatattaa agatgaattt     4860

gttgatagag gattagattt tatcaataaa aatctttcta aattagttaa aaaaggaaag     4920

atagaagaag ctactaaagt tgaaatctta actagaattt ccggaacagt tgaccttaat     4980

atggcagctg attgcgattt agttatagaa gcagctgttg aaagaatgga tattaaaaag     5040

cagatttttg ctgacttaga caatatatgc aagccagaaa caattcttgc atcaaataca     5100

tcatcacttt caataacaga agtggcatca gcaactaaaa gacctgataa ggttataggt     5160

atgcatttct ttaatccagc tcctgttatg aagcttgtag aggtaataag aggaatagct     5220

acatcacaag aaacttttga tgcagttaaa gagacatcta tagcaatagg aaaagatcct     5280

gtagaagtag cagaagcacc aggatttgtt gtaaatagaa tattaatacc aatgattaat     5340

gaagcagttg gtatattagc agaaggaata gcttcagtag aagacataga taaagctatg     5400

aaacttggag ctaatcaccc aatgggacca ttagaattag gtgattttat aggtcttgat     5460

atatgtcttg ctataatgga tgttttatac tcagaaactg gagattctaa gtatagacca     5520

catacattac ttaagaagta tgtaagagca ggatggcttg gaagaaaatc aggaaaaggt     5580

ttctacgatt attcaaaata agtttacagg atctgcaggg aggaggaaat catggagttg     5640

aacaacgtta ttctggagaa agaaggcaag gtggcggttg tcaccattaa ccgtccaaag     5700

gccctgaacg ctctgaactc ggataccctg aaagagatgg attacgttat tggcgagatt     5760

gagaatgaca gcgaagtgct ggctgtgatt ctgaccggtg cgggtgagaa gagctttgtc     5820

gcgggtgcgg acatcagcga gatgaaagaa atgaacacca tcgaaggccg taagttcggt     5880

attctgggca acaaggtgtt tcgtcgtctg gaactgctgg agaaacctgt cattgctgcc     5940

gtgaacggtt tcgcgctggg cggtggttgc gagatcgcta tgagctgcga tattcgtatc     6000

gcatcgtcca acgcacgctt tggtcaaccg gaggtcggtc tgggtatcac tccgggtttc     6060

ggcggtacgc aacgtctgag ccgcctggtt ggcatgggca tggcgaaaca gttgattttc     6120

acggcacaga acattaaggc ggatgaggcg ctgcgtattg gtctggtgaa taaggtcgtt     6180

gagccaagcg aactgatgaa taccgcgaaa gaaattgcga acaagatcgt tagcaatgcc     6240

ccggtggccg ttaagctgtc gaaacaggca atcaaccgtg gcatgcagtg tgacatcgac     6300

accgccctgg cgtttgagag cgaggcgttt ggtgagtgct tctccaccga ggaccaaaag     6360

gatgcgatga ccgcgttcat tgagaaacgc aagatcgagg gtttcaagaa tcgttaatag     6420

aggaggatag gaggttttca tatgattgtg aaaccgatgg tccgtaataa tatctgtctg     6480

aatgctcacc cgcagggctg taaaaaaggc gtggaagatc aaattgaata taccaaaaaa     6540

cgtattacgg cagaagtgaa agccggcgca aaagctccga aaaacgtgct ggttctgggt     6600

tgcagcaatg gctatggtct ggcttctcgc attaccgcgg cctttggcta cggtgcagct     6660

acgatcggcg ttagtttcga aaaagcaggt tccgaaacca aatatggcac gccgggttgg     6720

tacaacaatc tggcttttga tgaagcggcc aaacgtgaag gcctgtatag tgtcaccatt     6780

gatggtgacg cgttctccga tgaaattaaa gcacaggtga tcgaagaagc gaagaaaaaa     6840

ggcattaaat ttgacctgat cgtttacagc ctggcatctc cggtccgtac cgatccggac     6900

acgggtatca tgcataaatc tgtgctgaaa ccgtttggca aaaccttcac gggtaaaacc     6960

gttgatccgt tcacgggcga actgaaagaa attagcgcgg aaccggccaa cgatgaagaa     7020

gcagctgcga ccgtcaaagt gatgggcggt gaagactggg aacgttggat caaacagctg     7080

agtaaagaag gcctgctgga agaaggttgc attaccctgg cgtattccta catcggcccg     7140

gaagcaaccc aagctctgta tcgcaaaggc acgattggta aagcgaaaga acatctggaa     7200

gcgaccgccc accgtctgaa caaagaaaat ccgtcaatcc gcgccttcgt ttcggtcaat     7260

aaaggtctgg ttacccgtgc atcagctgtg attccggtta tcccgctgta cctggcatcg     7320

ctgtttaaag tcatgaaaga aaaaggcaac catgaaggtt gtattgaaca gatcacccgc     7380

ctgtatgccg aacgtctgta ccgcaaagat ggtacgattc cggtggacga agaaaatcgt     7440

attcgcatcg atgactggga actggaagaa gatgtccaaa aagccgtgag cgccctgatg     7500

gaaaaagtta ccggcgaaaa cgcggaatct ctgacggatc tggccggtta tcgtcacgac     7560

tttctggcga gtaatggttt tgatgttgaa ggcattaact acgaagctga agtggaacgc     7620

tttgatcgca tttgatctag agaattcgtc aacgaattca agcttgatat cattcaggac     7680

gagcctcaga ctccagcgta actggactga aaacaaacta aagcgccctt gtggcgcttt     7740

agttttgttc cgctcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga     7800

aatgtgcgcg cccgcgttcc tgctggcgct gggcctgttt ctggcgctgg acttcccgct     7860

gttccgtcag cagcttttcg cccacggcct tgatgatcgc ggcggccttg gcctgcatat     7920

cccgattcaa cggccccagg gcgtccagaa cgggcttcag gcgctcccga aggtctcggg     7980

ccgtctcttg ggcttgatcg gccttcttgc gcatctcacg cgctcctgcg gcggcctgta     8040

gggcaggctc atacccctgc cgaaccgctt ttgtcagccg gtcggccacg gcttccggcg     8100

tctcaacgcg ctttgagatt cccagctttt cggccaatcc ctgcggtgca taggcgcgtg     8160

gctcgaccgc ttgcgggctg atggtgacgt ggcccactgg tggccgctcc agggcctcgt     8220

agaacgcctg aatgcgcgtg tgacgtgcct tgctgccctc gatgccccgt tgcagcccta     8280

gatcggccac agcggccgca aacgtggtct ggtcgcgggt catctgcgct ttgttgccga     8340

tgaactcctt ggccgacagc ctgccgtcct gcgtcagcgg caccacgaac gcggtcatgt     8400

gcgggctggt ttcgtcacgg tggatgctgg ccgtcacgat gcgatccgcc ccgtacttgt     8460

ccgccagcca cttgtgcgcc ttctcgaaga acgccgcctg ctgttcttgg ctggccgact     8520

tccaccattc cgggctggcc gtcatgacgt actcgaccgc caacacagcg tccttgcgcc     8580

gcttctctgg cagcaactcg cgcagtcggc ccatcgcttc atcggtgctg ctggccgccc     8640

agtgctcgtt ctctggcgtc ctgctggcgt cagcgttggg cgtctcgcgc tcgcggtagg     8700

cgtgcttgag actggccgcc acgttgccca ttttcgccag cttcttgcat cgcatgatcg     8760

cgtatgccgc catgcctgcc cctccctttt ggtgtccaac cggctcgacg ggggcagcgc     8820

aaggcggtgc ctccggcggg ccactcaatg cttgagtata ctcactagac tttgcttcgc     8880

aaagtcgtga ccgcctacgg cggctgcggc gccctacggg cttgctctcc gggcttcgcc     8940

ctgcgcggtc gctgcgctc                                                  8959


<210>  21
<211>  5367
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(5367)
<223>  pET-28b(empty vector)

<400>  21
ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc       60

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc      120

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg      180

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca      240

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc      300

tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct      360

tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa      420

caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     2160

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     2220

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt     2280

attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac     2340

aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg     2400

gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     2460

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     2520

ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg     2580

tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga     2640

agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg     2700

gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa     2760

cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt     2820

tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt     2880

caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct     2940

gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac     3000

gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag     3060

cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc     3120

cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc     3180

atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag     3240

gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg     3300

ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg     3360

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac     3420

cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa     3480

tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac     3540

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     3600

gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac     3660

cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa     3720

atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta     3780

tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc     3840

gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag     3900

catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat     3960

cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga     4020

gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg     4080

ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg     4140

gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc     4200

atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt     4260

gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct     4320

ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag     4380

ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc     4440

cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt     4500

cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc     4560

atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc     4620

ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat     4680

ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc     4740

cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc     4800

cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc     4860

gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg     4920

cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa     4980

attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat     5040

tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca     5100

gcagcggcct ggtgccgcgc ggcagccata tggctagcat gactggtgga cagcaaatgg     5160

gtcgggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc     5220

accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca     5280

ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt     5340

tgctgaaagg aggaactata tccggat                                         5367


<210>  22
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-40 for gcvTHP amplification

<400>  22
ccggggatcg cggccgcgga ccgaacttat ccgccaggca atg                         43


<210>  23
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-41 for gcvTHP amplification

<400>  23
ttactggtat tcgctaatcg gctcgagcat cttgtcctca ttgaataagc g                51


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-42 for gcvTHP amplification

<400>  24
ctcgagccga ttagcgaata ccagtaattc                                        30


<210>  25
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-43 for gcvTHP amplification

<400>  25
ggatcgcggc cgctctagag ttcatcgtgc tgatcgattg c                           41


<210>  26
<211>  553
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 1 from example 12, 1553bp

<400>  26
ccggggatcg cggccgcgga ccgaacttat ccgccaggca atgggattaa acgatttgcc       60

tgaatggctg cgttaaaaat ttctcctctg ttgtttattt gatacccatc acactttcat      120

ctcccggttt tttcgccggg agattttcct catttgaaat aaactaattt cacctccgtt      180

ttcgcattat attttctaat gccattattt tttgatttag tgttttttga cattttttta      240

gctcttaata ttgtcttatt caaattgact ttctcatcac atcatctttg tatagaaact      300

ggtgtatttt ttggtttttt attctgtcgc gatttttgca ttttttaacc ataagctaat      360

gtgatgatca attttacctt atggttaaca gtctgtttcg gtggtaagtt caggcaaaag      420

agaacgattg cgttggggac cgggagtggc tccgatgctg ggtttcgtgg tgataatttc      480

accatgaaaa agttgtcagc cccgcttatt caatgaggac aagatgctcg agccgattag      540

cgaataccag taa                                                         553


<210>  27
<211>  547
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 2 from example 12, 1553bp

<400>  27
ctcgagccga ttagcgaata ccagtaattc actgattcga ctattttcta aaggcgcttc       60

ggcgcctttt tagtcagatg acaaagtaca aaagtgctca gacagtcccc tcgccccttt      120

ggggagaggg ttagggtgag gggaacaggc cggcactggc gcgaatattt accctcaccc      180

cggccctctc cctgaaaggg cgagggggaa aagcgtgcca acattgaaga ttgagccagt      240

ttgttagcaa tctcaaagat acgtcaacga attaattttt ctcggaaaaa caaatggcta      300

tagcacttgt gactggtggc agtcgcggca tcgggcgggc aactgcatta ctgttggcgc      360

aagaagggta tacggtggcg gttaattatc agcaaaacct ccacgcggcg caggaagtga      420

tgaacttaat aacgcaagcc ggtggcaaag cattcgtgct ccaggcggat atcagcgacg      480

aaaaccaggt cgttgcgatg tttacagcaa tcgatcagca cgatgaactc tagagcggcc      540

gcgatcc                                                                547


<210>  28
<211>  5667
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector pKO3

<400>  28
cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct       60

ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat      120

cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt      180

tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga      240

tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca      300

gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc      360

gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg      420

aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt      480

gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt      540

gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac      600

ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc      660

aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc      720

gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg      780

gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa      840

ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg      900

tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga      960

aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt     1020

atgtctattg ctggtctcgg tacccgggga tcgcggccgc ggaccggatc ctctagagcg     1080

gccgcgatcc tctagagtcg accggtggcg aatgggacgc gccctgtagc ggcgcattaa     1140

gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc     1200

ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag     1260

ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca     1320

aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc     1380

gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa     1440

cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct     1500

attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa     1560

cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt     1620

tttctaaata cattcaaata tgtatccgct caccgcgatc ctttttaacc catcacatat     1680

acctgccgtt cactattatt tagtgaaatg agatattatg atattttctg aattgtgatt     1740

aaaaaggcaa ctttatgccc atgcaacaga aactataaaa aatacagaga atgaaaagaa     1800

acagatagat tttttagttc tttaggcccg tagtctgcaa atccttttat gattttctat     1860

caaacaaaag aggaaaatag accagttgca atccaaacga gagtctaata gaatgaggtc     1920

gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc aagacctaaa atgtgtaaag     1980

ggcaaagtgt atactttggc gtcacccctt acatatttta ggtctttttt tattgtgcgt     2040

aactaacttg ccatcttcaa acaggagggc tggaagaagc agaccgctaa cacagtacat     2100

aaaaaaggag acatgaacga tgaacatcaa aaagtttgca aaacaagcaa cagtattaac     2160

ctttactacc gcactgctgg caggaggcgc aactcaagcg tttgcgaaag aaacgaacca     2220

aaagccatat aaggaaacat acggcatttc ccatattaca cgccatgata tgctgcaaat     2280

ccctgaacag caaaaaaatg aaaaatatca agttcctgag ttcgattcgt ccacaattaa     2340

aaatatctct tctgcaaaag gcctggacgt ttgggacagc tggccattac aaaacgctga     2400

cggcactgtc gcaaactatc acggctacca catcgtcttt gcattagccg gagatcctaa     2460

aaatgcggat gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga     2520

cagctggaaa aacgctggcc gcgtctttaa agacagcgac aaattcgatg caaatgattc     2580

tatcctaaaa gaccaaacac aagaatggtc aggttcagcc acatttacat ctgacggaaa     2640

aatccgttta ttctacactg atttctccgg taaacattac ggcaaacaaa cactgacaac     2700

tgcacaagtt aacgtatcag catcagacag ctctttgaac atcaacggtg tagaggatta     2760

taaatcaatc tttgacggtg acggaaaaac gtatcaaaat gtacagcagt tcatcgatga     2820

aggcaactac agctcaggcg acaaccatac gctgagagat cctcactacg tagaagataa     2880

aggccacaaa tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga     2940

agaatcttta tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag     3000

tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg     3060

tatgattgag ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc     3120

taacacagta acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta     3180

cctgttcact gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat     3240

ttacatgctt ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac     3300

tggccttgtg ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt     3360

cgctgtacct caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg     3420

attctacgca gacaaacaat caacgtttgc gccaagcttc ctgctgaaca tcaaaggcaa     3480

gaaaacatct gttgtcaaag acagcatcct tgaacaagga caattaacag ttaacaaata     3540

aaaacgcaaa agaaaatgcc gatattgact accggaagca gtgtgaccgt gtgcttctca     3600

aatgcctgat tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt     3660

tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca     3720

tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttaaa tgcaccaaaa     3780

actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac     3840

agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat     3900

gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat     3960

gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata     4020

cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt     4080

taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt     4140

tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc     4200

catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc     4260

aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca     4320

ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc     4380

atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa     4440

ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata     4500

ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc     4560

taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac     4620

caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac     4680

accataagca ttttccctac tgatgttcat catctgaacg tattggttat aagtgaacga     4740

taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca     4800

taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc     4860

tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata     4920

ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat     4980

ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa     5040

ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat     5100

aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact     5160

ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa     5220

cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa     5280

tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt     5340

tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat     5400

ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc     5460

gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc     5520

cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct     5580

aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtcggggatc     5640

gacgctctcc cttatgcgac tcctgca                                         5667


<210>  29
<211>  6693
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  knock-out vector pKO3 delta gcvTHP

<400>  29
cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct       60

ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat      120

cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt      180

tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga      240

tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca      300

gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc      360

gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg      420

aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt      480

gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt      540

gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac      600

ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc      660

aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc      720

gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg      780

gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa      840

ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg      900

tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga      960

aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt     1020

atgtctattg ctggtctcgg tacccgggga tcgcggccgc ggaccgaact tatccgccag     1080

gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa aatttctcct ctgttgttta     1140

tttgataccc atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga     1200

aataaactaa tttcacctcc gttttcgcat tatattttct aatgccatta ttttttgatt     1260

tagtgttttt tgacattttt ttagctctta atattgtctt attcaaattg actttctcat     1320

cacatcatct ttgtatagaa actggtgtat tttttggttt tttattctgt cgcgattttt     1380

gcatttttta accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt     1440

tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg     1500

ctgggtttcg tggtgataat ttcaccatga aaaagttgtc agccccgctt attcaatgag     1560

gacaagatgc tcgagccgat tagcgaatac cagtaattca ctgattcgac tattttctaa     1620

aggcgcttcg gcgccttttt agtcagatga caaagtacaa aagtgctcag acagtcccct     1680

cgcccctttg gggagagggt tagggtgagg ggaacaggcc ggcactggcg cgaatattta     1740

ccctcacccc ggccctctcc ctgaaagggc gagggggaaa agcgtgccaa cattgaagat     1800

tgagccagtt tgttagcaat ctcaaagata cgtcaacgaa ttaatttttc tcggaaaaac     1860

aaatggctat agcacttgtg actggtggca gtcgcggcat cgggcgggca actgcattac     1920

tgttggcgca agaagggtat acggtggcgg ttaattatca gcaaaacctc cacgcggcgc     1980

aggaagtgat gaacttaata acgcaagccg gtggcaaagc attcgtgctc caggcggata     2040

tcagcgacga aaaccaggtc gttgcgatgt ttacagcaat cgatcagcac gatgaactct     2100

agagcggccg cgatcctcta gagtcgaccg gtggcgaatg ggacgcgccc tgtagcggcg     2160

cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc     2220

tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc     2280

gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg     2340

accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg     2400

tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg     2460

gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt     2520

cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa     2580

tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg     2640

tttatttttc taaatacatt caaatatgta tccgctcacc gcgatccttt ttaacccatc     2700

acatatacct gccgttcact attatttagt gaaatgagat attatgatat tttctgaatt     2760

gtgattaaaa aggcaacttt atgcccatgc aacagaaact ataaaaaata cagagaatga     2820

aaagaaacag atagattttt tagttcttta ggcccgtagt ctgcaaatcc ttttatgatt     2880

ttctatcaaa caaaagagga aaatagacca gttgcaatcc aaacgagagt ctaatagaat     2940

gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa gcaggcaaga cctaaaatgt     3000

gtaaagggca aagtgtatac tttggcgtca ccccttacat attttaggtc tttttttatt     3060

gtgcgtaact aacttgccat cttcaaacag gagggctgga agaagcagac cgctaacaca     3120

gtacataaaa aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt     3180

attaaccttt actaccgcac tgctggcagg aggcgcaact caagcgtttg cgaaagaaac     3240

gaaccaaaag ccatataagg aaacatacgg catttcccat attacacgcc atgatatgct     3300

gcaaatccct gaacagcaaa aaaatgaaaa atatcaagtt cctgagttcg attcgtccac     3360

aattaaaaat atctcttctg caaaaggcct ggacgtttgg gacagctggc cattacaaaa     3420

cgctgacggc actgtcgcaa actatcacgg ctaccacatc gtctttgcat tagccggaga     3480

tcctaaaaat gcggatgaca catcgattta catgttctat caaaaagtcg gcgaaacttc     3540

tattgacagc tggaaaaacg ctggccgcgt ctttaaagac agcgacaaat tcgatgcaaa     3600

tgattctatc ctaaaagacc aaacacaaga atggtcaggt tcagccacat ttacatctga     3660

cggaaaaatc cgtttattct acactgattt ctccggtaaa cattacggca aacaaacact     3720

gacaactgca caagttaacg tatcagcatc agacagctct ttgaacatca acggtgtaga     3780

ggattataaa tcaatctttg acggtgacgg aaaaacgtat caaaatgtac agcagttcat     3840

cgatgaaggc aactacagct caggcgacaa ccatacgctg agagatcctc actacgtaga     3900

agataaaggc cacaaatact tagtatttga agcaaacact ggaactgaag atggctacca     3960

aggcgaagaa tctttattta acaaagcata ctatggcaaa agcacatcat tcttccgtca     4020

agaaagtcaa aaacttctgc aaagcgataa aaaacgcacg gctgagttag caaacggcgc     4080

tctcggtatg attgagctaa acgatgatta cacactgaaa aaagtgatga aaccgctgat     4140

tgcatctaac acagtaacag atgaaattga acgcgcgaac gtctttaaaa tgaacggcaa     4200

atggtacctg ttcactgact cccgcggatc aaaaatgacg attgacggca ttacgtctaa     4260

cgatatttac atgcttggtt atgtttctaa ttctttaact ggcccataca agccgctgaa     4320

caaaactggc cttgtgttaa aaatggatct tgatcctaac gatgtaacct ttacttactc     4380

acacttcgct gtacctcaag cgaaaggaaa caatgtcgtg attacaagct atatgacaaa     4440

cagaggattc tacgcagaca aacaatcaac gtttgcgcca agcttcctgc tgaacatcaa     4500

aggcaagaaa acatctgttg tcaaagacag catccttgaa caaggacaat taacagttaa     4560

caaataaaaa cgcaaaagaa aatgccgata ttgactaccg gaagcagtgt gaccgtgtgc     4620

ttctcaaatg cctgattcag gctgtctatg tgtgactgtt gagctgtaac aagttgtctc     4680

aggtgttcaa tttcatgttc tagttgcttt gttttactgg tttcacctgt tctattaggt     4740

gttacatgct gttcatctgt tacattgtcg atctgttcat ggtgaacagc tttaaatgca     4800

ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat     4860

atggacagtt ttccctttga tatgtaacgg tgaacagttg ttctactttt gtttgttagt     4920

cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc     4980

cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag     5040

atcatactta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt     5100

tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg aatctgatgt     5160

aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac     5220

gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg     5280

cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa     5340

aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt     5400

aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt     5460

taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag     5520

attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac     5580

taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc     5640

tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc     5700

taatacacca taagcatttt ccctactgat gttcatcatc tgaacgtatt ggttataagt     5760

gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac     5820

acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc     5880

atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc     5940

actataccaa ttgagatggg ctagtcaatg ataattacta gtccttttcc tttgagttgt     6000

gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc     6060

tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta     6120

tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc     6180

actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct     6240

acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc     6300

gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca     6360

ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc     6420

ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct     6480

cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt     6540

tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga     6600

ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtcg     6660

gggatcgacg ctctccctta tgcgactcct gca                                  6693


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence after deletion of gcvTHP

<400>  30
atgctcgagc cgattagcga ataccagtaa                                        30


<210>  31
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-44 for amplifying the upstream region of 
       glyA

<400>  31
gggatcgcgg ccgcggaccg agggcttcac gttgatcg                               38


<210>  32
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-45 for amplifying the upstream region of 
       glyA

<400>  32
tatgcgtaaa ccgggtaacg ctcgagcatc cgcatctcct gactc                       45


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-46 for amplifying the downstream region of 
       glyA

<400>  33
cgttacccgg tttacgcata ag                                                22


<210>  34
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-47 for amplifying the downstream region of 
       glyA

<400>  34
ggatcgcggc cgctctagag atcaccagct gggttgatc                              39


<210>  35
<211>  546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 1 from Example 14

<400>  35
gggatcgcgg ccgcggaccg agggcttcac gttgatcgcc attacgctgg ttactcatgt       60

taaaaatttc tttgagttct gggttatgag taaacatacg gtcgtagaaa tgggcggtta      120

actttggccc cgtttccacc agtaaaggga tggtggcttt tactgtagcg atggtttgag      180

cgtcaagcat atggtcttcc tttttttgca tcttaattga tgtatctcaa atgcatctta      240

taaaaaatag ccctgcaatg taaatggttc tttggtgttt ttcagaaaga atgtgatgaa      300

gtgaaaaatt tgcatcacaa acctgaaaag aaatccgttt ccggttgcaa gctctttatt      360

ctccaaagcc ttgcgtagcc tgaaggtaat cgtttgcgta aattcctttg tcaagacctg      420

ttatcgcaca atgattcggt tatactgttc gccgttgtcc aacaggaccg cctataaagg      480

ccaaaaattt tattgttagc tgagtcagga gatgcggatg ctcgagcgtt acccggttta      540

cgcata                                                                 546


<210>  36
<211>  520
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 2 from Example 14

<400>  36
cgttacccgg tttacgcata agcgaaacgg tgatttgctg tcaatgtgct cgttgttcat       60

gccggatgcg gcgtgaacgc cttatccggc ctacaaaact ttgcaaattc aatatattgc      120

aatctccgtg taggcctgat aagcgtagcg catcaggcaa tttttcgttt atgatcatca      180

aggcttcctt cgggaagcct ttctacgtta tcgcgccatc aaatctgtcg taactgcgcc      240

tcaacataca aatagccaat tcccagcacc tgttgtgcgc ggcttaattg cccaaagcca      300

atttgcgtcg ctttcacgct cggtccttcg tcgcggtcga acgggttaaa tcctgtctgg      360

cgaataatag tattaagcca gttctcacca aaggcacaac tgcgaccgta caaccaaatc      420

tgattaatat tgaggatgtt gaggaagtta tacagactca aaccaatggc attggcagag      480

cgatcaaccc agctggtgat ctctagagcg gccgcgatcc                            520


<210>  37
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-52

<400>  37
actgaggatc cgggatcgcg gccgcg                                            26


<210>  38
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-53

<400>  38
actgaggatc cggatcgcgg ccgctctaga g                                      31


<210>  39
<211>  1052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 3 from Example 14

<400>  39
gatccgggat cgcggccgcg gaccgagggc ttcacgttga tcgccattac gctggttact       60

catgttaaaa atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc      120

ggttaacttt ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt      180

ttgagcgtca agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca      240

tcttataaaa aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg      300

atgaagtgaa aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct      360

ttattctcca aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag      420

acctgttatc gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat      480

aaaggccaaa aattttattg ttagctgagt caggagatgc ggatgctcga gcgttacccg      540

gtttacgcat aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc      600

ggcgtgaacg ccttatccgg cctacaaaac tttgcaaatt caatatattg caatctccgt      660

gtaggcctga taagcgtagc gcatcaggca atttttcgtt tatgatcatc aaggcttcct      720

tcgggaagcc tttctacgtt atcgcgccat caaatctgtc gtaactgcgc ctcaacatac      780

aaatagccaa ttcccagcac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtc      840

gctttcacgc tcggtccttc gtcgcggtcg aacgggttaa atcctgtctg gcgaataata      900

gtattaagcc agttctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata      960

ttgaggatgt tgaggaagtt atacagactc aaaccaatgg cattggcaga gcgatcaacc     1020

cagctggtga tctctagagc ggccgcgatc cg                                   1052


<210>  40
<211>  6719
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKO3 delta glyA

<400>  40
cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct       60

ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat      120

cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt      180

tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga      240

tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca      300

gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc      360

gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg      420

aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt      480

gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt      540

gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac      600

ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc      660

aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc      720

gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg      780

gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa      840

ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg      900

tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga      960

aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt     1020

atgtctattg ctggtctcgg tacccgggga tcgcggccgc ggaccggatc cgggatcgcg     1080

gccgcggacc gagggcttca cgttgatcgc cattacgctg gttactcatg ttaaaaattt     1140

ctttgagttc tgggttatga gtaaacatac ggtcgtagaa atgggcggtt aactttggcc     1200

ccgtttccac cagtaaaggg atggtggctt ttactgtagc gatggtttga gcgtcaagca     1260

tatggtcttc ctttttttgc atcttaattg atgtatctca aatgcatctt ataaaaaata     1320

gccctgcaat gtaaatggtt ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat     1380

ttgcatcaca aacctgaaaa gaaatccgtt tccggttgca agctctttat tctccaaagc     1440

cttgcgtagc ctgaaggtaa tcgtttgcgt aaattccttt gtcaagacct gttatcgcac     1500

aatgattcgg ttatactgtt cgccgttgtc caacaggacc gcctataaag gccaaaaatt     1560

ttattgttag ctgagtcagg agatgcggat gctcgagcgt tacccggttt acgcataagc     1620

gaaacggtga tttgctgtca atgtgctcgt tgttcatgcc ggatgcggcg tgaacgcctt     1680

atccggccta caaaactttg caaattcaat atattgcaat ctccgtgtag gcctgataag     1740

cgtagcgcat caggcaattt ttcgtttatg atcatcaagg cttccttcgg gaagcctttc     1800

tacgttatcg cgccatcaaa tctgtcgtaa ctgcgcctca acatacaaat agccaattcc     1860

cagcacctgt tgtgcgcggc ttaattgccc aaagccaatt tgcgtcgctt tcacgctcgg     1920

tccttcgtcg cggtcgaacg ggttaaatcc tgtctggcga ataatagtat taagccagtt     1980

ctcaccaaag gcacaactgc gaccgtacaa ccaaatctga ttaatattga ggatgttgag     2040

gaagttatac agactcaaac caatggcatt ggcagagcga tcaacccagc tggtgatctc     2100

tagagcggcc gcgatccgga tcctctagag cggccgcgat cctctagagt cgaccggtgg     2160

cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag     2220

cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt     2280

tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt     2340

ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg     2400

tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt     2460

taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt     2520

tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca     2580

aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg     2640

ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg     2700

ctcaccgcga tcctttttaa cccatcacat atacctgccg ttcactatta tttagtgaaa     2760

tgagatatta tgatattttc tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca     2820

gaaactataa aaaatacaga gaatgaaaag aaacagatag attttttagt tctttaggcc     2880

cgtagtctgc aaatcctttt atgattttct atcaaacaaa agaggaaaat agaccagttg     2940

caatccaaac gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact     3000

gataaagcag gcaagaccta aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc     3060

ttacatattt taggtctttt tttattgtgc gtaactaact tgccatcttc aaacaggagg     3120

gctggaagaa gcagaccgct aacacagtac ataaaaaagg agacatgaac gatgaacatc     3180

aaaaagtttg caaaacaagc aacagtatta acctttacta ccgcactgct ggcaggaggc     3240

gcaactcaag cgtttgcgaa agaaacgaac caaaagccat ataaggaaac atacggcatt     3300

tcccatatta cacgccatga tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat     3360

caagttcctg agttcgattc gtccacaatt aaaaatatct cttctgcaaa aggcctggac     3420

gtttgggaca gctggccatt acaaaacgct gacggcactg tcgcaaacta tcacggctac     3480

cacatcgtct ttgcattagc cggagatcct aaaaatgcgg atgacacatc gatttacatg     3540

ttctatcaaa aagtcggcga aacttctatt gacagctgga aaaacgctgg ccgcgtcttt     3600

aaagacagcg acaaattcga tgcaaatgat tctatcctaa aagaccaaac acaagaatgg     3660

tcaggttcag ccacatttac atctgacgga aaaatccgtt tattctacac tgatttctcc     3720

ggtaaacatt acggcaaaca aacactgaca actgcacaag ttaacgtatc agcatcagac     3780

agctctttga acatcaacgg tgtagaggat tataaatcaa tctttgacgg tgacggaaaa     3840

acgtatcaaa atgtacagca gttcatcgat gaaggcaact acagctcagg cgacaaccat     3900

acgctgagag atcctcacta cgtagaagat aaaggccaca aatacttagt atttgaagca     3960

aacactggaa ctgaagatgg ctaccaaggc gaagaatctt tatttaacaa agcatactat     4020

ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa     4080

cgcacggctg agttagcaaa cggcgctctc ggtatgattg agctaaacga tgattacaca     4140

ctgaaaaaag tgatgaaacc gctgattgca tctaacacag taacagatga aattgaacgc     4200

gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca ctgactcccg cggatcaaaa     4260

atgacgattg acggcattac gtctaacgat atttacatgc ttggttatgt ttctaattct     4320

ttaactggcc catacaagcc gctgaacaaa actggccttg tgttaaaaat ggatcttgat     4380

cctaacgatg taacctttac ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat     4440

gtcgtgatta caagctatat gacaaacaga ggattctacg cagacaaaca atcaacgttt     4500

gcgccaagct tcctgctgaa catcaaaggc aagaaaacat ctgttgtcaa agacagcatc     4560

cttgaacaag gacaattaac agttaacaaa taaaaacgca aaagaaaatg ccgatattga     4620

ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg attcaggctg tctatgtgtg     4680

actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt     4740

tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct     4800

gttcatggtg aacagcttta aatgcaccaa aaactcgtaa aagctctgat gtatctatct     4860

tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa     4920

cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa     4980

gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc     5040

gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt     5100

gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt     5160

ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt     5220

atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa     5280

atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg     5340

tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag     5400

ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg     5460

tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg     5520

ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga     5580

taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag     5640

tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc     5700

gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct actgatgttc     5760

atcatctgaa cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt     5820

tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt     5880

aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat     5940

ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa     6000

ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga     6060

aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt     6120

gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga     6180

atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg     6240

atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt     6300

agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca     6360

cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa     6420

tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat     6480

acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc     6540

tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct gaccacttcg     6600

gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat     6660

caacaggctt acccgtctta ctgtcgggga tcgacgctct cccttatgcg actcctgca      6719


<210>  41
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence after deletion of glyA

<400>  41
atgctcgagc gttacccggt ttacgcataa                                        30


<210>  42
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ltaE-UP_fw

<400>  42
ggatcgcggc cgcggaccgc aggcgacaga gccagaacg                              39


<210>  43
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ltAE-UP-XhoI_rev

<400>  43
ctctccttaa cgcgccagga actcgagcat ggcatgtcct tattatg                     47


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ltaE-DOWN_fw

<400>  44
ttcctggcgc gttaaggaga g                                                 21


<210>  45
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ltaE-DOWN_rev

<400>  45
ggatcgcggc cgctctagag tagaccatat cgcgcatgac                             40


<210>  46
<211>  540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 4 from Example 15

<400>  46
ggatcgcggc cgcggaccgc aggcgacaga gccagaacgt caggtggtcg ccatgtgcgg       60

cgatggcggt tttagcatgt tgatgggcga tttcctctca gtagtgcaga tgaaactgcc      120

agtgaaaatt gtcgtcttta acaacagcgt gctgggcttt gtggcgatgg agatgaaagc      180

tggtggctat ttgactgacg gcaccgaact acacgacaca aactttgccc gcattgccga      240

agcgtgcggc attacgggta tccgtgtaga aaaagcgtct gaagttgatg aagccctgca      300

acgcgccttc tccatcgacg gtccggtgtt ggtggatgtg gtggtcgcca aagaagagtt      360

agccattcca ccgcagatca aactcgaaca ggccaaaggt ttcagcctgt atatgctgcg      420

cgcaatcatc agcggacgcg gtgatgaagt gatcgaactg gcgaaaacaa actggctaag      480

gtaaaaaggg tggcatttcc cgtcataata aggacatgcc atgctcgagt tcctggcgcg      540


<210>  47
<211>  536
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 5 from Example 15

<400>  47
ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt ttagttctcg gtgccagtgg       60

ctacattggt cagcatctgg tgcgcacact cagccagcaa gggcatcaga tcctggcggc      120

ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca aacgtcagtt gccataaagt      180

cgatctcagc tggccggata acctaccggc cctgttgcag gatatcgata cggtctattt      240

tctggtgcac agcatgggcg aaggcggcga ttttatcgct caggagcgcc aggtggctct      300

caacgtccgc gatgcgctac gtgaagtacc agttaagcaa ttaatctttc ttagttcgtt      360

gcaggccccg ccacatgagc agtcggatca tctgcgtgct cgtcaggcta cggcggacat      420

tctgcgtgaa gcgaatgtac ccgtgaccga actgcgggcc ggaattatcg ttggcgcagg      480

ttccgcggcg ttcgaagtca tgcgcgatat ggtctactct agagcggccg cgatcc          536


<210>  48
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide o-MO-54 from Example 15

<400>  48
actgaggatc cggatcgcgg ccgcgg                                            26


<210>  49
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligoneucleotide o-MO-55 from Example 15

<400>  49
actgaggatc cggatcgcgg ccgctctag                                         29


<210>  50
<211>  1071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product 6 from Example 15

<400>  50
gatccggatc gcggccgcgg accgcaggcg acagagccag aacgtcaggt ggtcgccatg       60

tgcggcgatg gcggttttag catgttgatg ggcgatttcc tctcagtagt gcagatgaaa      120

ctgccagtga aaattgtcgt ctttaacaac agcgtgctgg gctttgtggc gatggagatg      180

aaagctggtg gctatttgac tgacggcacc gaactacacg acacaaactt tgcccgcatt      240

gccgaagcgt gcggcattac gggtatccgt gtagaaaaag cgtctgaagt tgatgaagcc      300

ctgcaacgcg ccttctccat cgacggtccg gtgttggtgg atgtggtggt cgccaaagaa      360

gagttagcca ttccaccgca gatcaaactc gaacaggcca aaggtttcag cctgtatatg      420

ctgcgcgcaa tcatcagcgg acgcggtgat gaagtgatcg aactggcgaa aacaaactgg      480

ctaaggtaaa aagggtggca tttcccgtca taataaggac atgccatgct cgagttcctg      540

gcgcgttaag gagagagacg tgccgcaacg cattttagtt ctcggtgcca gtggctacat      600

tggtcagcat ctggtgcgca cactcagcca gcaagggcat cagatcctgg cggcggcacg      660

tcatgtcgac aggcttgcaa agctgcaact ggcaaacgtc agttgccata aagtcgatct      720

cagctggccg gataacctac cggccctgtt gcaggatatc gatacggtct attttctggt      780

gcacagcatg ggcgaaggcg gcgattttat cgctcaggag cgccaggtgg ctctcaacgt      840

ccgcgatgcg ctacgtgaag taccagttaa gcaattaatc tttcttagtt cgttgcaggc      900

cccgccacat gagcagtcgg atcatctgcg tgctcgtcag gctacggcgg acattctgcg      960

tgaagcgaat gtacccgtga ccgaactgcg ggccggaatt atcgttggcg caggttccgc     1020

ggcgttcgaa gtcatgcgcg atatggtcta ctctagagcg gccgcgatcc g              1071


<210>  51
<211>  6738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKO3 delta ltaE

<400>  51
cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct       60

ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat      120

cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt      180

tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga      240

tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca      300

gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc      360

gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg      420

aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt      480

gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt      540

gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac      600

ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc      660

aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc      720

gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg      780

gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa      840

ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg      900

tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga      960

aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt     1020

atgtctattg ctggtctcgg tacccgggga tcgcggccgc ggaccggatc cggatcgcgg     1080

ccgcggaccg caggcgacag agccagaacg tcaggtggtc gccatgtgcg gcgatggcgg     1140

ttttagcatg ttgatgggcg atttcctctc agtagtgcag atgaaactgc cagtgaaaat     1200

tgtcgtcttt aacaacagcg tgctgggctt tgtggcgatg gagatgaaag ctggtggcta     1260

tttgactgac ggcaccgaac tacacgacac aaactttgcc cgcattgccg aagcgtgcgg     1320

cattacgggt atccgtgtag aaaaagcgtc tgaagttgat gaagccctgc aacgcgcctt     1380

ctccatcgac ggtccggtgt tggtggatgt ggtggtcgcc aaagaagagt tagccattcc     1440

accgcagatc aaactcgaac aggccaaagg tttcagcctg tatatgctgc gcgcaatcat     1500

cagcggacgc ggtgatgaag tgatcgaact ggcgaaaaca aactggctaa ggtaaaaagg     1560

gtggcatttc ccgtcataat aaggacatgc catgctcgag ttcctggcgc gttaaggaga     1620

gagacgtgcc gcaacgcatt ttagttctcg gtgccagtgg ctacattggt cagcatctgg     1680

tgcgcacact cagccagcaa gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc     1740

ttgcaaagct gcaactggca aacgtcagtt gccataaagt cgatctcagc tggccggata     1800

acctaccggc cctgttgcag gatatcgata cggtctattt tctggtgcac agcatgggcg     1860

aaggcggcga ttttatcgct caggagcgcc aggtggctct caacgtccgc gatgcgctac     1920

gtgaagtacc agttaagcaa ttaatctttc ttagttcgtt gcaggccccg ccacatgagc     1980

agtcggatca tctgcgtgct cgtcaggcta cggcggacat tctgcgtgaa gcgaatgtac     2040

ccgtgaccga actgcgggcc ggaattatcg ttggcgcagg ttccgcggcg ttcgaagtca     2100

tgcgcgatat ggtctactct agagcggccg cgatccggat cctctagagc ggccgcgatc     2160

ctctagagtc gaccggtggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg     2220

gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt     2280

tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc     2340

gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg     2400

attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga     2460

cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc     2520

ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa     2580

aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa     2640

tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat     2700

acattcaaat atgtatccgc tcaccgcgat cctttttaac ccatcacata tacctgccgt     2760

tcactattat ttagtgaaat gagatattat gatattttct gaattgtgat taaaaaggca     2820

actttatgcc catgcaacag aaactataaa aaatacagag aatgaaaaga aacagataga     2880

ttttttagtt ctttaggccc gtagtctgca aatcctttta tgattttcta tcaaacaaaa     2940

gaggaaaata gaccagttgc aatccaaacg agagtctaat agaatgaggt cgaaaagtaa     3000

atcgcgcggg tttgttactg ataaagcagg caagacctaa aatgtgtaaa gggcaaagtg     3060

tatactttgg cgtcacccct tacatatttt aggtcttttt ttattgtgcg taactaactt     3120

gccatcttca aacaggaggg ctggaagaag cagaccgcta acacagtaca taaaaaagga     3180

gacatgaacg atgaacatca aaaagtttgc aaaacaagca acagtattaa cctttactac     3240

cgcactgctg gcaggaggcg caactcaagc gtttgcgaaa gaaacgaacc aaaagccata     3300

taaggaaaca tacggcattt cccatattac acgccatgat atgctgcaaa tccctgaaca     3360

gcaaaaaaat gaaaaatatc aagttcctga gttcgattcg tccacaatta aaaatatctc     3420

ttctgcaaaa ggcctggacg tttgggacag ctggccatta caaaacgctg acggcactgt     3480

cgcaaactat cacggctacc acatcgtctt tgcattagcc ggagatccta aaaatgcgga     3540

tgacacatcg atttacatgt tctatcaaaa agtcggcgaa acttctattg acagctggaa     3600

aaacgctggc cgcgtcttta aagacagcga caaattcgat gcaaatgatt ctatcctaaa     3660

agaccaaaca caagaatggt caggttcagc cacatttaca tctgacggaa aaatccgttt     3720

attctacact gatttctccg gtaaacatta cggcaaacaa acactgacaa ctgcacaagt     3780

taacgtatca gcatcagaca gctctttgaa catcaacggt gtagaggatt ataaatcaat     3840

ctttgacggt gacggaaaaa cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta     3900

cagctcaggc gacaaccata cgctgagaga tcctcactac gtagaagata aaggccacaa     3960

atacttagta tttgaagcaa acactggaac tgaagatggc taccaaggcg aagaatcttt     4020

atttaacaaa gcatactatg gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact     4080

tctgcaaagc gataaaaaac gcacggctga gttagcaaac ggcgctctcg gtatgattga     4140

gctaaacgat gattacacac tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt     4200

aacagatgaa attgaacgcg cgaacgtctt taaaatgaac ggcaaatggt acctgttcac     4260

tgactcccgc ggatcaaaaa tgacgattga cggcattacg tctaacgata tttacatgct     4320

tggttatgtt tctaattctt taactggccc atacaagccg ctgaacaaaa ctggccttgt     4380

gttaaaaatg gatcttgatc ctaacgatgt aacctttact tactcacact tcgctgtacc     4440

tcaagcgaaa ggaaacaatg tcgtgattac aagctatatg acaaacagag gattctacgc     4500

agacaaacaa tcaacgtttg cgccaagctt cctgctgaac atcaaaggca agaaaacatc     4560

tgttgtcaaa gacagcatcc ttgaacaagg acaattaaca gttaacaaat aaaaacgcaa     4620

aagaaaatgc cgatattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga     4680

ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca     4740

tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca     4800

tctgttacat tgtcgatctg ttcatggtga acagctttaa atgcaccaaa aactcgtaaa     4860

agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc     4920

tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg     4980

atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag     5040

tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg     5100

catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc     5160

gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct gatgtaatgg ttgttggtat     5220

tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct     5280

atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa     5340

tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag     5400

ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct     5460

aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata     5520

aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa     5580

tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc     5640

gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt     5700

cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc     5760

attttcccta ctgatgttca tcatctgaac gtattggtta taagtgaacg ataccgtccg     5820

ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag     5880

cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac     5940

aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag     6000

atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt     6060

ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag     6120

acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga     6180

ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt     6240

tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta     6300

aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt     6360

tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc     6420

tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg     6480

caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg     6540

cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt     6600

ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc     6660

agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtcggggat cgacgctctc     6720

ccttatgcga ctcctgca                                                   6738


<210>  52
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence after deletion of ltaE

<400>  52
atgctcgagt tcctggcgcg ttaa                                              24


<210>  53
<211>  294
<212>  PRT
<213>  Nomocharis pardanthina

<400>  53

Met Leu Val Leu His Asn Ala Gln Lys Leu Gln Ile Leu Tyr Lys Ser 
1               5                   10                  15      


Leu Glu Lys Ser Ile Pro Glu Ser Ile Lys Val Tyr Gly Ala Ile Phe 
            20                  25                  30          


Asn Ile Lys Asp Lys Asn Pro Phe Asn Met Glu Val Leu Val Asp Ala 
        35                  40                  45              


Trp Pro Asp Tyr Gln Ile Val Ile Thr Arg Pro Gln Lys Gln Glu Met 
    50                  55                  60                  


Lys Asp Asp Gln Asp His Tyr Thr Asn Thr Tyr His Ile Phe Thr Lys 
65                  70                  75                  80  


Ala Pro Asp Lys Leu Glu Glu Val Leu Ser Tyr Ser Ser Val Ile Asn 
                85                  90                  95      


Trp Glu Gln Thr Leu Gln Ile Gln Gly Cys Gln Glu Gly Leu Asp Glu 
            100                 105                 110         


Ala Ile Arg Lys Ala Ala Thr Ser Lys Ser Val Gln Val Asp Tyr Met 
        115                 120                 125             


Lys Thr Met Leu Phe Ile Pro Glu Leu Pro Lys Lys His Lys Thr Ser 
    130                 135                 140                 


Ser Asn Glu Lys Met Glu Leu Phe Lys Val Asp Asp Asp Asn Lys Glu 
145                 150                 155                 160 


Gly Asn Phe Ser Asn Met Phe Leu Asp Ala Ser His Ala Gly Leu Val 
                165                 170                 175     


Asn Glu His Trp Ala Phe Gly Lys Asn Glu Arg Ser Leu Lys Tyr Ile 
            180                 185                 190         


Glu Arg Cys Leu Gln Asp Phe Leu Gly Phe Gly Val Leu Gly Pro Glu 
        195                 200                 205             


Gly Gln Leu Val Ser Trp Ile Val Met Glu Gln Ser Cys Glu Leu Arg 
    210                 215                 220                 


Met Gly Tyr Thr Val Pro Lys Tyr Arg His Gln Gly Asn Met Leu Gln 
225                 230                 235                 240 


Ile Gly Tyr His Phe Glu Lys Tyr Leu Ser Gln Lys Glu Ile Pro Phe 
                245                 250                 255     


Tyr Phe His Val Ala Asp Asn Asn Glu Lys Ser Leu Gln Ala Leu Lys 
            260                 265                 270         


Asn Leu Gly Phe Lys Ile Cys Pro Cys Gly Trp His Gln Trp Lys Cys 
        275                 280                 285             


Thr Pro Lys Lys Tyr Cys 
    290                 


<210>  54
<211>  297
<212>  PRT
<213>  Saimiri boliviensis

<400>  54

Met Phe Val Leu His Asp Thr Gln Ile Leu Gln Thr Leu Tyr Lys Ser 
1               5                   10                  15      


Leu Glu Lys Arg Asn Pro Glu Ser Ile Lys Val Tyr Gly Ala Ile Phe 
            20                  25                  30          


Asn Ile Lys Asn Lys Asn Pro Phe Asn Met Glu Val Leu Val Asp Ala 
        35                  40                  45              


Trp Pro Asp Tyr Gln Thr Val Ile Ile Arg Pro Lys Lys Gln Glu Met 
    50                  55                  60                  


Lys Asp Asp Gln Asp His Tyr Thr Asn Thr Tyr His Ile Phe Thr Lys 
65                  70                  75                  80  


Ala Pro Asp Lys Leu Glu Glu Ile Leu Ser Cys Ser Glu Val Ile Asn 
                85                  90                  95      


Trp Glu Gln Thr Phe Gln Ile Gln Gly Cys Gln Glu Gly Leu Asn Glu 
            100                 105                 110         


Ala Ile Arg Lys Val Ala Ala Ser Lys Thr Val Gln Val Asp Tyr Val 
        115                 120                 125             


Lys Thr Val Leu Phe Lys Pro Glu Leu Leu Lys Glu His Lys Thr Ser 
    130                 135                 140                 


Ser Asn Asp Lys Met Glu Leu Phe Glu Met His Lys Val Asp Asp Asn 
145                 150                 155                 160 


Asn Lys Lys Arg Asn Phe Ser Ser Met Phe Leu Asp Ala Ser His Ala 
                165                 170                 175     


Gly Leu Ile Asn Glu His Trp Ala Phe Gly Lys Asn Glu Arg Ser Leu 
            180                 185                 190         


Lys Tyr Thr Glu Arg Cys Leu Gln Asp Phe Leu Gly Phe Gly Val Leu 
        195                 200                 205             


Gly Pro Glu Gly Glu Leu Ile Ser Trp Val Val Met Glu Gln Ser Cys 
    210                 215                 220                 


Glu Leu Arg Met Gly Tyr Thr Ile Pro Lys Tyr Arg Asn Gln Arg His 
225                 230                 235                 240 


Met Ser Gln Ile Leu Cys Tyr Leu Glu Lys Tyr Leu Ser Gln Lys Glu 
                245                 250                 255     


Ile Pro Phe Tyr Ile His Val Ala Asp Tyr Asn Val Ala Gly Leu Gln 
            260                 265                 270         


Ile Met Lys Asn Met Gly Tyr Met Ile Cys Pro Cys Gly Trp His Gln 
        275                 280                 285             


Trp Lys Cys Thr Pro Lys Lys Tyr Cys 
    290                 295         


<210>  55
<211>  298
<212>  PRT
<213>  Felis catus

<400>  55

Met Phe Val Leu His Glu Ser Gln Lys Leu His Ile Leu Tyr Glu Ser 
1               5                   10                  15      


Leu Glu Lys Ser Ile Pro Glu Ser Leu Lys Val Tyr Gly Ala Val Phe 
            20                  25                  30          


Asn Ile Lys Asn Lys Asn Pro Phe Asn Met Glu Val Leu Val Asp Ala 
        35                  40                  45              


Trp Pro Asp Tyr Gln Ile Val Ile Thr Arg Pro Gln Arg Glu Glu Met 
    50                  55                  60                  


Lys Asp Asp Leu Asp His Tyr Thr Asn Thr Tyr Gln Ile Phe Thr Lys 
65                  70                  75                  80  


Ala Pro Asp Lys Leu Glu Glu Val Leu Ala Gln Pro Gln Leu Ile Asn 
                85                  90                  95      


Trp Asp Gln Val Phe Gln Ile Gln Gly Cys Gln Glu Ser Leu Gln Glu 
            100                 105                 110         


Ala Ile Arg Lys Ile Ser Ala Ser Lys Ser Val Arg Val Asp Tyr Val 
        115                 120                 125             


Lys Thr Ile Arg Ile Met Thr Glu Met Pro Val Lys Pro Asn Asp Asp 
    130                 135                 140                 


Gln Val Asp Val Met Glu Ser Phe Lys Met Pro Lys Val Asp Asp Asp 
145                 150                 155                 160 


Thr Lys Lys Gly Ser Phe Gln Asn Ile Ser Leu Asp Pro Ser His Ala 
                165                 170                 175     


Gly Leu Val Asn Glu Gln Trp Ala Phe Gly Lys Asn Asp Arg Ser Leu 
            180                 185                 190         


Lys Tyr Ile Glu Arg Cys Leu Gln Asn Phe Pro Gly Phe Gly Val Leu 
        195                 200                 205             


Gly Pro Glu Gly Arg Leu Ile Ser Trp Ile Val Met Glu Gln Ser Cys 
    210                 215                 220                 


Glu Met Arg Met Gly Tyr Thr Val Pro Glu Tyr Arg Lys His Gly Tyr 
225                 230                 235                 240 


Met Lys Lys Thr Ser Val His Leu Met Asn Tyr Val Ile Gln Lys Lys 
                245                 250                 255     


Val Pro Cys Tyr Phe His Val Ser Glu His Lys Glu Glu Tyr Asn Gln 
            260                 265                 270         


Ala Leu Arg Asp Leu Lys Phe Lys Ala Ala Ser Cys Gly Trp His Gln 
        275                 280                 285             


Trp Lys Cys Ser Pro Glu Lys Tyr Val Asp 
    290                 295             


<210>  56
<211>  299
<212>  PRT
<213>  Bos taurus

<400>  56

Met Phe Val Leu Arg Glu Pro Gln Gln Leu Gln Ile Leu Tyr Glu Ser 
1               5                   10                  15      


Leu Glu Glu Ser Ile Pro Glu Ser Leu Lys Val Tyr Gly Thr Leu Phe 
            20                  25                  30          


His Ile Arg Asn Lys Asn Pro Phe Asn Leu Glu Val Leu Val Asp Ala 
        35                  40                  45              


Trp Pro Glu Tyr Gln Thr Val Val Ile Arg Pro Gln Lys Glu Glu Met 
    50                  55                  60                  


Lys Asp Asp Leu Asp Tyr Tyr Thr Asn Thr Tyr His Ile Phe Thr Lys 
65                  70                  75                  80  


Ala Pro Asp Lys Leu Glu Glu Val Leu Ala Cys Pro Gln Val Ile Asn 
                85                  90                  95      


Trp Glu Gln Ala Phe Gln Ile Gln Gly Cys Gln Glu Ser Leu Gly Glu 
            100                 105                 110         


Ala Ile Gln Lys Val Ala Ala Ser Lys Ser Val Gln Val Asp Tyr Leu 
        115                 120                 125             


Arg Thr Val Leu Phe Val Phe Glu Lys Pro Thr Leu Glu Ala Ser Ser 
    130                 135                 140                 


Gly Asp Lys Met Asp Leu Met Lys Leu Leu Lys Ile Pro Lys Val Leu 
145                 150                 155                 160 


Glu Asp Lys Arg Lys Glu Thr Phe Gln Asn Ile Phe Leu Asp Val Ser 
                165                 170                 175     


His Ala Arg Leu Val Asn Glu His Trp Glu Leu Gly Lys Asn Glu Lys 
            180                 185                 190         


Ser Leu Lys Tyr Val Glu Arg Cys Leu Gln Asn Phe Ala Gly Phe Gly 
        195                 200                 205             


Val Leu Ser Ser Glu Gly Lys Pro Ile Ser Trp Phe Leu Thr Glu Gln 
    210                 215                 220                 


Ser Cys Glu Ile Arg Met Gly Tyr Thr Phe Pro Lys Tyr Arg Gly Gln 
225                 230                 235                 240 


Gly His Met Trp Gln Met Gly Cys His Ser Ile Ala Tyr Phe Phe Ser 
                245                 250                 255     


Lys Lys Ile Pro Phe Tyr Ile His Ala Ala Glu Asp Lys Glu Lys Thr 
            260                 265                 270         


Gln Gln Thr Leu Leu Ser Phe Gly Phe Lys Asn Val Leu Cys Gly Trp 
        275                 280                 285             


His Gln Trp Lys Cys Thr Pro Lys Lys Tyr Cys 
    290                 295                 


<210>  57
<211>  296
<212>  PRT
<213>  Mus musculus

<400>  57

Met Ile Val Pro Leu Gln Gly Ala Gln Met Leu Gln Met Leu Glu Lys 
1               5                   10                  15      


Ser Leu Arg Lys Tyr Leu Pro Glu Ser Leu Lys Val Tyr Gly Thr Val 
            20                  25                  30          


Tyr His Met Ile His Gly Asn Pro Phe Asn Leu Lys Ala Leu Val Asp 
        35                  40                  45              


Lys Trp Pro Asp Phe Asn Thr Val Val Val Arg Pro Gln Glu Gln Glu 
    50                  55                  60                  


Met Thr Asp Asp Leu Asp Phe Tyr Ile Asn Thr Tyr Gln Val Tyr Ser 
65                  70                  75                  80  


Lys Asp Pro Gln Asn Cys Gln Glu Phe Leu Glu Ser Ser Glu Val Ile 
                85                  90                  95      


Asn Trp Lys Gln His Leu Gln Ile Gln Ser Ser Gln Ser His Leu Asn 
            100                 105                 110         


Lys Thr Ile Gln Asn Leu Ala Ser Ile Gln Ser Phe Gln Ile Lys His 
        115                 120                 125             


Ser Glu Asn Ile Leu Tyr Val Ser Ser Glu Thr Ile Lys Lys Leu Phe 
    130                 135                 140                 


Pro Ser Leu Leu Asp Thr Lys Asn Leu Ser Thr Gly Ser Gly Lys Pro 
145                 150                 155                 160 


Lys Ala Ile Asp Gln Asp Lys Phe Lys Leu Ser Ser Leu Asp Val Val 
                165                 170                 175     


His Ala Ala Leu Val Asn Lys Phe Trp Leu Phe Gly Gly Asn Glu Arg 
            180                 185                 190         


Ser Gln Arg Phe Ile Glu Arg Cys Ile Lys Asn Phe Pro Ser Ser Cys 
        195                 200                 205             


Val Leu Gly Pro Glu Gly Thr Pro Ala Ser Trp Thr Leu Met Asp Gln 
    210                 215                 220                 


Thr Gly Glu Met Arg Met Gly Gly Thr Met Pro Glu Tyr Arg Leu Gln 
225                 230                 235                 240 


Gly Leu Val Ser Phe Val Val His Ser Gln Asp Gln Ile Met Thr Lys 
                245                 250                 255     


Arg Gly Tyr Pro Val Tyr Ser His Thr Glu Lys Ser Asn Ile Ala Met 
            260                 265                 270         


Gln Lys Met Ser Tyr Thr Leu Gln His Leu Pro Met Pro Cys Ala Trp 
        275                 280                 285             


Asn Gln Trp Lys Cys Met Pro Met 
    290                 295     


