                         SEQUENCE LISTING

<110>  Trustees of Dartmouth College
       Griswold, Karl E.
       Zhao, Hongliang
 
<120>  UNGLYCOSYLATED LYSOSTAPHIN VARIANT PROTEIN

<130>  DC0523WO

<150>  US 61/862,599
<151>  2013-08-06

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  246
<212>  PRT
<213>  Staphylococcus simulans

<400>  1

Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
1               5                   10                  15      


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
            20                  25                  30          


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
        35                  40                  45              


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
    50                  55                  60                  


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
65                  70                  75                  80  


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
                85                  90                  95      


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
            100                 105                 110         


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
        115                 120                 125             


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
    130                 135                 140                 


Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr 
145                 150                 155                 160 


Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp 
                165                 170                 175     


Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly 
            180                 185                 190         


Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu Val Met Lys Gln 
        195                 200                 205             


Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser Gly Gln Arg Ile 
    210                 215                 220                 


Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val 
225                 230                 235                 240 


Leu Trp Gly Thr Ile Lys 
                245     


<210>  2
<211>  741
<212>  DNA
<213>  Staphylococcus simulans

<400>  2
gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac       60

ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat      120

attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat      180

tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat      240

atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc      300

ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt      360

aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat      420

ggaaaagcag gtggtacagt aactccaacg cccaatacag gttggaaaac aaacaaatat      480

ggcacactat ataaatcaga gtcagctagc ttcacaccta atacagatat aataacaaga      540

acgactggtc catttagaag catgccgcag tcaggagtct taaaagcagg tcaaacaatt      600

cattatgatg aagtgatgaa acaagacggt catgtttggg taggttatac aggtaacagt      660

ggccaacgta tttacttgcc tgtaagaaca tggaataaat ctactaatac tttaggtgtt      720

ctttggggaa ctataaagtg a                                                741


<210>  3
<211>  741
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  3
gctgctaccc acgagcactc cgctcaatgg ttgaacaact acaagaaggg ttacggttac       60

ggtccatacc cattgggtat caacggtggt atgcactacg gtgttgactt cttcatgaac      120

atcggtaccc cagtcaaggc tatctcctcc ggtaagatcg tcgaggctgg ttggtccaac      180

tacggtggtg gtaaccaaat cggtttgatc gagaacgacg gtgtccacag acaatggtac      240

atgcacttgt ccaagtacaa cgtcaaggtc ggtgactacg tcaaggctgg tcaaatcatc      300

ggttggtccg gttccaccgg ttactccacc gctccacact tgcacttcca aagaatggtc      360

aactccttct ccaactccac cgctcaagac ccaatgccat tcttgaagtc cgctggttac      420

ggtaaggctg gtggtaccgt caccccaacc ccaaacaccg gttggaagac caacaagtac      480

ggtaccttgt acaagtccga gtccgcttcc ttcaccccaa acaccgacat catcaccaga      540

accaccggtc cattcagatc catgccacaa tccggtgtct tgaaggctgg tcaaaccatc      600

cactacgacg aggtcatgaa gcaagacggt cacgtctggg tcggttacac cggtaactcc      660

ggtcaaagaa tctacttgcc agtcagaacc tggaacaagt ccaccaacac cttgggtgtc      720

ttgtggggta ccatcaagta a                                                741


<210>  4
<211>  246
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<400>  4

Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
1               5                   10                  15      


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
            20                  25                  30          


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
        35                  40                  45              


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
    50                  55                  60                  


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
65                  70                  75                  80  


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
                85                  90                  95      


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
            100                 105                 110         


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Pro Thr Ala 
        115                 120                 125             


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
    130                 135                 140                 


Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr 
145                 150                 155                 160 


Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp 
                165                 170                 175     


Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly 
            180                 185                 190         


Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu Val Met Lys Gln 
        195                 200                 205             


Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser Gly Gln Arg Ile 
    210                 215                 220                 


Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val 
225                 230                 235                 240 


Leu Trp Gly Thr Ile Lys 
                245     


<210>  5
<211>  741
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  5
gctgctaccc acgagcactc cgctcaatgg ttgaacaact acaagaaggg ttacggttac       60

ggtccatacc cattgggtat caacggtggt atgcactacg gtgttgactt cttcatgaac      120

atcggtaccc cagtcaaggc tatctcctcc ggtaagatcg tcgaggctgg ttggtccaac      180

tacggtggtg gtaaccaaat cggtttgatc gagaacgacg gtgtccacag acaatggtac      240

atgcacttgt ccaagtacaa cgtcaaggtc ggtgactacg tcaaggctgg tcaaatcatc      300

ggttggtccg gttccaccgg ttactccacc gctccacact tgcacttcca aagaatggtc      360

aactccttct ccaacccaac cgctcaagac ccaatgccat tcttgaagtc cgctggttac      420

ggtaaggctg gtggtaccgt caccccaacc ccaaacaccg gttggaagac caacaagtac      480

ggtaccttgt acaagtccga gtccgcttcc ttcaccccaa acaccgacat catcaccaga      540

accaccggtc cattcagatc catgccacaa tccggtgtct tgaaggctgg tcaaaccatc      600

cactacgacg aggtcatgaa gcaagacggt cacgtctggg tcggttacac cggtaactcc      660

ggtcaaagaa tctacttgcc agtcagaacc tggaacaagt ccaccaacac cttgggtgtc      720

ttgtggggta ccatcaagta a                                                741


<210>  6
<211>  246
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<400>  6

Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
1               5                   10                  15      


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
            20                  25                  30          


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
        35                  40                  45              


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
    50                  55                  60                  


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
65                  70                  75                  80  


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
                85                  90                  95      


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
            100                 105                 110         


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Ala Ala 
        115                 120                 125             


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
    130                 135                 140                 


Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr 
145                 150                 155                 160 


Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp 
                165                 170                 175     


Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly 
            180                 185                 190         


Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu Val Met Lys Gln 
        195                 200                 205             


Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser Gly Gln Arg Ile 
    210                 215                 220                 


Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val 
225                 230                 235                 240 


Leu Trp Gly Thr Ile Lys 
                245     


<210>  7
<211>  741
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  7
gctgctaccc acgagcactc cgctcaatgg ttgaacaact acaagaaggg ttacggttac       60

ggtccatacc cattgggtat caacggtggt atgcactacg gtgttgactt cttcatgaac      120

atcggtaccc cagtcaaggc tatctcctcc ggtaagatcg tcgaggctgg ttggtccaac      180

tacggtggtg gtaaccaaat cggtttgatc gagaacgacg gtgtccacag acaatggtac      240

atgcacttgt ccaagtacaa cgtcaaggtc ggtgactacg tcaaggctgg tcaaatcatc      300

ggttggtccg gttccaccgg ttactccacc gctccacact tgcacttcca aagaatggtc      360

aactccttct ccaactccgc tgctcaagac ccaatgccat tcttgaagtc cgctggttac      420

ggtaaggctg gtggtaccgt caccccaacc ccaaacaccg gttggaagac caacaagtac      480

ggtaccttgt acaagtccga gtccgcttcc ttcaccccaa acaccgacat catcaccaga      540

accaccggtc cattcagatc catgccacaa tccggtgtct tgaaggctgg tcaaaccatc      600

cactacgacg aggtcatgaa gcaagacggt cacgtctggg tcggttacac cggtaactcc      660

ggtcaaagaa tctacttgcc agtcagaacc tggaacaagt ccaccaacac cttgggtgtc      720

ttgtggggta ccatcaagta a                                                741


<210>  8
<211>  246
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<400>  8

Ala Ala Thr His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
1               5                   10                  15      


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
            20                  25                  30          


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
        35                  40                  45              


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
    50                  55                  60                  


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
65                  70                  75                  80  


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
                85                  90                  95      


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
            100                 105                 110         


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
        115                 120                 125             


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
    130                 135                 140                 


Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr 
145                 150                 155                 160 


Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp 
                165                 170                 175     


Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly 
            180                 185                 190         


Val Leu Lys Ala Gly Gln Thr Ile His Tyr Asp Glu Val Met Lys Gln 
        195                 200                 205             


Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser Gly Gln Arg Ile 
    210                 215                 220                 


Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val 
225                 230                 235                 240 


Leu Trp Gly Thr Ile Lys 
                245     


<210>  9
<211>  15
<212>  DNA
<213>  Staphylococcus simulans

<400>  9
aataattaca aaaaa                                                        15


<210>  10
<211>  17
<212>  DNA
<213>  Staphylococcus simulans

<400>  10
atttttttat gaatatt                                                      17


<210>  11
<211>  16
<212>  DNA
<213>  Staphylococcus simulans

<400>  11
taaatataat gttaaa                                                       16


<210>  12
<211>  49
<212>  DNA
<213>  Staphylococcus simulans

<400>  12
tagacaatgg tatatgcatc taagtaaata taatgttaaa gtaggagat                   49


<210>  13
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  13
cagacaatgg tacatgcact tgtccaagta caacgtcaag gtcggtgac                   49


<210>  14
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  14
cagacaatgg tacatgcact tgtctaaata taatgttaaa gtcggtgac                   49


<210>  15
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  15
tagacaatgg tatatgcatc taagcaagta caacgtcaag gtaggagat                   49


<210>  16
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  16
atcgctcgag aaaagagctg caacacat                                          28


<210>  17
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  17
cgatgaattc ttactttata gttcccca                                          28


<210>  18
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  18
atcgctcgag aaaagagctg ctacccac                                          28


<210>  19
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  19
cgatgaattc ttacttgatg gtacccca                                          28


<210>  20
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  20
caaagaatgg ttaattcatt ttcccaatcc accgctcaag ac                          42


<210>  21
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  21
gtcttgagcg gtggattggg aaaatgaatt aaccattctt tg                          42


<210>  22
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  22
caaagaatgg tcaactcctt ctcaaattca actgcccaag at                          42


<210>  23
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  23
atcttgggca gttgaatttg agaaggagtt gaccattctt tg                          42


<210>  24
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  24
gaagctggtt ggagtaatta cggaggtggt aaccaaatcg gtttgatc                    48


<210>  25
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  25
gatcaaaccg atttggttac cacctccgta attactccaa ccagcttc                    48


<210>  26
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  26
gaggctggtt ggtccaacta cggtggaggt aatcaaatag gtcttatt                    48


<210>  27
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  27
aataagacct atttgattac ctccaccgta gttggaccaa ccagcctc                    48


<210>  28
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  28
aaatataatg ttaaagtagg agattacgtc aaggctggtc aaatcatc                    48


<210>  29
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  29
gatgatttga ccagccttga cgtaatctcc tactttaaca ttatattt                    48


<210>  30
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  30
aagtacaacg tcaaggtcgg tgactatgtc aaagctggtc aaataatc                    48


<210>  31
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  31
gattatttga ccagctttga catagtcacc gaccttgacg ttgtactt                    48


<210>  32
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  32
ggtcttattg aaaatgatgg agtgcacaga caatggtaca tgcacttg                    48


<210>  33
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  33
caagtgcatg taccattgtc tgtgcactcc atcattttca ataagacc                    48


<210>  34
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  34
ggtttgatcg agaacgacgg tgtccataga caatggtata tgcatcta                    48


<210>  35
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  35
tagatgcata taccattgtc tatggacacc gtcgttctcg atcaaacc                    48


<210>  36
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  36
aactccttct cccaatccac cgctcaa                                           27


<210>  37
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  37
ttgagcggtg gattgggaga aggagtt                                           27


<210>  38
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  38
aactccttct ccgactccac cgctcaa                                           27


<210>  39
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  39
ttgagcggtg gagtcggaga aggagtt                                           27


<210>  40
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  40
aactccttct cctcctccac cgctcaa                                           27


<210>  41
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  41
ttgagcggtg gaggaggaga aggagtt                                           27


<210>  42
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  42
aactccttct ccaacccaac cgctcaa                                           27


<210>  43
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  43
ttgagcggtt gggttggaga aggagtt                                           27


<210>  44
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  44
ttctccaact ccgctgctca agaccca                                           27


<210>  45
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  45
tgggtcttga gcagcggagt tggagaa                                           27


<210>  46
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  46
atgagatttc cttcaatttt tactg                                             25


<210>  47
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  47
caccgtcgtt ctcgatcaaa ccga                                              24


<210>  48
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  48
cttgatggta ccccacaaga cacc                                              24


<210>  49
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  49
ctccatcatt ttcaataaga ccta                                              24


<210>  50
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  50
ctttatagtt ccccaaagaa cacc                                              24


<210>  51
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  51
attgccggaa gattggcaaa cttg                                              24


<210>  52
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  52
aaaacgattt gctttctagc acgg                                              24


