                         SEQUENCE LISTING

<110>  Aronora, Inc.
       AbSci, LLC
       Gruber, Andras
       McClain, Sean
       Valasek, Mark
 
<120>  COORDINATED COEXPRESSION OF THROMBIN

<130>  AbSciA-01PCT

<150>  PCT/US13/53562
<151>  2013-08-05

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  622
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala His Val Arg Gly Leu Gln Leu Pro Gly Cys Leu Ala Leu Ala 
1               5                   10                  15      


Ala Leu Cys Ser Leu Val His Ser Gln His Val Phe Leu Ala Pro Gln 
            20                  25                  30          


Gln Ala Arg Ser Leu Leu Gln Arg Val Arg Arg Ala Asn Thr Phe Leu 
        35                  40                  45              


Glu Glu Val Arg Lys Gly Asn Leu Glu Arg Glu Cys Val Glu Glu Thr 
    50                  55                  60                  


Cys Ser Tyr Glu Glu Ala Phe Glu Ala Leu Glu Ser Ser Thr Ala Thr 
65                  70                  75                  80  


Asp Val Phe Trp Ala Lys Tyr Thr Ala Cys Glu Thr Ala Arg Thr Pro 
                85                  90                  95      


Arg Asp Lys Leu Ala Ala Cys Leu Glu Gly Asn Cys Ala Glu Gly Leu 
            100                 105                 110         


Gly Thr Asn Tyr Arg Gly His Val Asn Ile Thr Arg Ser Gly Ile Glu 
        115                 120                 125             


Cys Gln Leu Trp Arg Ser Arg Tyr Pro His Lys Pro Glu Ile Asn Ser 
    130                 135                 140                 


Thr Thr His Pro Gly Ala Asp Leu Gln Glu Asn Phe Cys Arg Asn Pro 
145                 150                 155                 160 


Asp Ser Ser Thr Thr Gly Pro Trp Cys Tyr Thr Thr Asp Pro Thr Val 
                165                 170                 175     


Arg Arg Gln Glu Cys Ser Ile Pro Val Cys Gly Gln Asp Gln Val Thr 
            180                 185                 190         


Val Ala Met Thr Pro Arg Ser Glu Gly Ser Ser Val Asn Leu Ser Pro 
        195                 200                 205             


Pro Leu Glu Gln Cys Val Pro Asp Arg Gly Gln Gln Tyr Gln Gly Arg 
    210                 215                 220                 


Leu Ala Val Thr Thr His Gly Leu Pro Cys Leu Ala Trp Ala Ser Ala 
225                 230                 235                 240 


Gln Ala Lys Ala Leu Ser Lys His Gln Asp Phe Asn Ser Ala Val Gln 
                245                 250                 255     


Leu Val Glu Asn Phe Cys Arg Asn Pro Asp Gly Asp Glu Glu Gly Val 
            260                 265                 270         


Trp Cys Tyr Val Ala Gly Lys Pro Gly Asp Phe Gly Tyr Cys Asp Leu 
        275                 280                 285             


Asn Tyr Cys Glu Glu Ala Val Glu Glu Glu Thr Gly Asp Gly Leu Asp 
    290                 295                 300                 


Glu Asp Ser Asp Arg Ala Ile Glu Gly Arg Thr Ala Thr Ser Glu Tyr 
305                 310                 315                 320 


Gln Thr Phe Phe Asn Pro Arg Thr Phe Gly Ser Gly Glu Ala Asp Cys 
                325                 330                 335     


Gly Leu Arg Pro Leu Phe Glu Lys Lys Ser Leu Glu Asp Lys Thr Glu 
            340                 345                 350         


Arg Glu Leu Leu Glu Ser Tyr Ile Asp Gly Arg Ile Val Glu Gly Ser 
        355                 360                 365             


Asp Ala Glu Ile Gly Met Ser Pro Trp Gln Val Met Leu Phe Arg Lys 
    370                 375                 380                 


Ser Pro Gln Glu Leu Leu Cys Gly Ala Ser Leu Ile Ser Asp Arg Trp 
385                 390                 395                 400 


Val Leu Thr Ala Ala His Cys Leu Leu Tyr Pro Pro Trp Asp Lys Asn 
                405                 410                 415     


Phe Thr Glu Asn Asp Leu Leu Val Arg Ile Gly Lys His Ser Arg Thr 
            420                 425                 430         


Arg Tyr Glu Arg Asn Ile Glu Lys Ile Ser Met Leu Glu Lys Ile Tyr 
        435                 440                 445             


Ile His Pro Arg Tyr Asn Trp Arg Glu Asn Leu Asp Arg Asp Ile Ala 
    450                 455                 460                 


Leu Met Lys Leu Lys Lys Pro Val Ala Phe Ser Asp Tyr Ile His Pro 
465                 470                 475                 480 


Val Cys Leu Pro Asp Arg Glu Thr Ala Ala Ser Leu Leu Gln Ala Gly 
                485                 490                 495     


Tyr Lys Gly Arg Val Thr Gly Trp Gly Asn Leu Lys Glu Thr Trp Thr 
            500                 505                 510         


Ala Asn Val Gly Lys Gly Gln Pro Ser Val Leu Gln Val Val Asn Leu 
        515                 520                 525             


Pro Ile Val Glu Arg Pro Val Cys Lys Asp Ser Thr Arg Ile Arg Ile 
    530                 535                 540                 


Thr Asp Asn Met Phe Cys Ala Gly Tyr Lys Pro Asp Glu Gly Lys Arg 
545                 550                 555                 560 


Gly Asp Ala Cys Glu Gly Asp Ser Gly Gly Pro Phe Val Met Lys Ser 
                565                 570                 575     


Pro Phe Asn Asn Arg Trp Tyr Gln Met Gly Ile Val Ser Trp Gly Glu 
            580                 585                 590         


Gly Cys Asp Arg Asp Gly Lys Tyr Gly Phe Tyr Thr His Val Phe Arg 
        595                 600                 605             


Leu Lys Lys Trp Ile Gln Lys Val Ile Asp Gln Phe Gly Glu 
    610                 615                 620         


<210>  2
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized coding sequence for human thrombin A chain (with 
       methionine residue added to the N-terminal end)

<400>  2
gctagcagga ggaattcacc atgactttcg gtagcggcga agcagattgc ggtttgcgtc       60

cattatttga gaagaaaagc ctggaagata aaaccgagcg cgaactgctg gagtcctaca      120

tcgacggccg ttaagtcgac                                                  140


<210>  3
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human thrombin A chain (with methionine residue added to the 
       N-terminal end)

<400>  3

Met Thr Phe Gly Ser Gly Glu Ala Asp Cys Gly Leu Arg Pro Leu Phe 
1               5                   10                  15      


Glu Lys Lys Ser Leu Glu Asp Lys Thr Glu Arg Glu Leu Leu Glu Ser 
            20                  25                  30          


Tyr Ile Asp Gly Arg 
        35          


<210>  4
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human thrombin B chain (with methionine residue added to the 
       N-terminal end)

<400>  4

Met Ile Val Glu Gly Ser Asp Ala Glu Ile Gly Met Ser Pro Trp Gln 
1               5                   10                  15      


Val Met Leu Phe Arg Lys Ser Pro Gln Glu Leu Leu Cys Gly Ala Ser 
            20                  25                  30          


Leu Ile Ser Asp Arg Trp Val Leu Thr Ala Ala His Cys Leu Leu Tyr 
        35                  40                  45              


Pro Pro Trp Asp Lys Asn Phe Thr Glu Asn Asp Leu Leu Val Arg Ile 
    50                  55                  60                  


Gly Lys His Ser Arg Thr Arg Tyr Glu Arg Asn Ile Glu Lys Ile Ser 
65                  70                  75                  80  


Met Leu Glu Lys Ile Tyr Ile His Pro Arg Tyr Asn Trp Arg Glu Asn 
                85                  90                  95      


Leu Asp Arg Asp Ile Ala Leu Met Lys Leu Lys Lys Pro Val Ala Phe 
            100                 105                 110         


Ser Asp Tyr Ile His Pro Val Cys Leu Pro Asp Arg Glu Thr Ala Ala 
        115                 120                 125             


Ser Leu Leu Gln Ala Gly Tyr Lys Gly Arg Val Thr Gly Trp Gly Asn 
    130                 135                 140                 


Leu Lys Glu Thr Trp Thr Ala Asn Val Gly Lys Gly Gln Pro Ser Val 
145                 150                 155                 160 


Leu Gln Val Val Asn Leu Pro Ile Val Glu Arg Pro Val Cys Lys Asp 
                165                 170                 175     


Ser Thr Arg Ile Arg Ile Thr Asp Asn Met Phe Cys Ala Gly Tyr Lys 
            180                 185                 190         


Pro Asp Glu Gly Lys Arg Gly Asp Ala Cys Glu Gly Asp Ser Gly Gly 
        195                 200                 205             


Pro Phe Val Met Lys Ser Pro Phe Asn Asn Arg Trp Tyr Gln Met Gly 
    210                 215                 220                 


Ile Val Ser Trp Gly Glu Gly Cys Asp Arg Asp Gly Lys Tyr Gly Phe 
225                 230                 235                 240 


Tyr Thr His Val Phe Arg Leu Lys Lys Trp Ile Gln Lys Val Ile Asp 
                245                 250                 255     


Gln Phe Gly Glu 
            260 


<210>  5
<211>  809
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized coding sequence for human W215A E217A thrombin B chain 
       (with methionine residue added to the N-terminal end)

<400>  5
gctagcagga ggaattcacc atgattgttg aaggttcgga cgcagaaatc ggtatgtcac       60

catggcaagt tatgttgttt cgcaaaagcc cgcaagagct gctgtgtggt gcctccctga      120

tttccgaccg ctgggtcctg accgcagcgc actgtctgct gtatccgccg tgggataaga      180

atttcaccga gaatgacctg ttggtgcgca ttggcaaaca cagccgtacg cgctacgaac      240

gtaacatcga gaagatcagc atgctggaga aaatctacat tcaccctcgt tataactggc      300

gtgagaactt ggaccgtgac atcgctctga tgaagctgaa gaaaccggtt gcgttcagcg      360

attacattca tccggtttgt ctgccggatc gcgagactgc cgcgagcctg ttacaagccg      420

gctataaggg tcgtgtaacg ggctggggta atctgaagga aacctggacc gcgaatgtcg      480

gtaaaggtca gccgagcgtg ctgcaagtgg ttaatctgcc gattgttgaa cgtccggtct      540

gcaaggatag cacccgcatt cgtatcacgg acaacatgtt ttgcgcaggc tacaagccag      600

atgaaggcaa gcgtggtgat gcttgcgagg gcgacagcgg tggcccgttt gtgatgaaat      660

ctccgttcaa caaccgttgg taccagatgg gtatcgtgag cgcgggtgcg ggttgcgacc      720

gtgacggtaa atacggcttc tatacccatg tgtttcgcct gaaaaagtgg atccagaaag      780

tcattgatca gttcggcgag taagtcgac                                        809


<210>  6
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human W215A E217A thrombin B chain (with methionine residue added
       to the N-terminal end)

<400>  6

Met Ile Val Glu Gly Ser Asp Ala Glu Ile Gly Met Ser Pro Trp Gln 
1               5                   10                  15      


Val Met Leu Phe Arg Lys Ser Pro Gln Glu Leu Leu Cys Gly Ala Ser 
            20                  25                  30          


Leu Ile Ser Asp Arg Trp Val Leu Thr Ala Ala His Cys Leu Leu Tyr 
        35                  40                  45              


Pro Pro Trp Asp Lys Asn Phe Thr Glu Asn Asp Leu Leu Val Arg Ile 
    50                  55                  60                  


Gly Lys His Ser Arg Thr Arg Tyr Glu Arg Asn Ile Glu Lys Ile Ser 
65                  70                  75                  80  


Met Leu Glu Lys Ile Tyr Ile His Pro Arg Tyr Asn Trp Arg Glu Asn 
                85                  90                  95      


Leu Asp Arg Asp Ile Ala Leu Met Lys Leu Lys Lys Pro Val Ala Phe 
            100                 105                 110         


Ser Asp Tyr Ile His Pro Val Cys Leu Pro Asp Arg Glu Thr Ala Ala 
        115                 120                 125             


Ser Leu Leu Gln Ala Gly Tyr Lys Gly Arg Val Thr Gly Trp Gly Asn 
    130                 135                 140                 


Leu Lys Glu Thr Trp Thr Ala Asn Val Gly Lys Gly Gln Pro Ser Val 
145                 150                 155                 160 


Leu Gln Val Val Asn Leu Pro Ile Val Glu Arg Pro Val Cys Lys Asp 
                165                 170                 175     


Ser Thr Arg Ile Arg Ile Thr Asp Asn Met Phe Cys Ala Gly Tyr Lys 
            180                 185                 190         


Pro Asp Glu Gly Lys Arg Gly Asp Ala Cys Glu Gly Asp Ser Gly Gly 
        195                 200                 205             


Pro Phe Val Met Lys Ser Pro Phe Asn Asn Arg Trp Tyr Gln Met Gly 
    210                 215                 220                 


Ile Val Ser Ala Gly Ala Gly Cys Asp Arg Asp Gly Lys Tyr Gly Phe 
225                 230                 235                 240 


Tyr Thr His Val Phe Arg Leu Lys Lys Trp Ile Gln Lys Val Ile Asp 
                245                 250                 255     


Gln Phe Gly Glu 
            260 


<210>  7
<211>  179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized coding sequence for human thrombin A chain, with 14 
       amino acids added to the N-terminal end

<400>  7
gctagcagga ggaattcacc atgacggcaa cgagcgagta ccagaccttt ttcgacggta       60

gaacctttgg cagcggcgaa gcggattgcg gtctgcgtcc gctgttcgag aagaaatccc      120

tggaagataa aaccgagcgc gagttgctgg aaagctatat cgacggtcgt taagtcgac       179


<210>  8
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human thrombin A chain, with 14 amino acids added to the 
       N-terminal end

<400>  8

Met Thr Ala Thr Ser Glu Tyr Gln Thr Phe Phe Asp Gly Arg Thr Phe 
1               5                   10                  15      


Gly Ser Gly Glu Ala Asp Cys Gly Leu Arg Pro Leu Phe Glu Lys Lys 
            20                  25                  30          


Ser Leu Glu Asp Lys Thr Glu Arg Glu Leu Leu Glu Ser Tyr Ile Asp 
        35                  40                  45              


Gly Arg 
    50  


<210>  9
<211>  956
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized coding sequence for human thrombin A / W215A E217A B 
       chains (W215A E217A prethrombin-2), with 14 amino acids added to 
       the N-terminal end

<400>  9
gctagcagga ggaattcacc atgacggcaa ctagcgaata ccaaactttt tttgacggtc       60

gtaccttcgg cagcggcgag gctgactgtg gcctgcgccc actgtttgaa aagaaaagcc      120

tggaagataa aaccgagcgt gagctgctgg agtcttacat cgacggtcgt attgttgaag      180

gttcggacgc agaaatcggt atgtcaccat ggcaagttat gttgtttcgc aaaagcccgc      240

aagagctgct gtgtggtgcc tccctgattt ccgaccgctg ggtcctgacc gcagcgcact      300

gtctgctgta tccgccgtgg gataagaatt tcaccgagaa tgacctgttg gtgcgcattg      360

gcaaacacag ccgtacgcgc tacgaacgta acatcgagaa gatcagcatg ctggagaaaa      420

tctacattca ccctcgttat aactggcgtg agaacttgga ccgtgacatc gctctgatga      480

agctgaagaa accggttgcg ttcagcgatt acattcatcc ggtttgtctg ccggatcgcg      540

agactgccgc gagcctgtta caagccggct ataagggtcg tgtaacgggc tggggtaatc      600

tgaaggaaac ctggaccgcg aatgtcggta aaggtcagcc gagcgtgctg caagtggtta      660

atctgccgat tgttgaacgt ccggtctgca aggatagcac ccgcattcgt atcacggaca      720

acatgttttg cgcaggctac aagccagatg aaggcaagcg tggtgatgct tgcgagggcg      780

acagcggtgg cccgtttgtg atgaaatctc cgttcaacaa ccgttggtac cagatgggta      840

tcgtgagcgc gggtgcgggt tgcgaccgtg acggtaaata cggcttctat acccatgtgt      900

ttcgcctgaa aaagtggatc cagaaagtca ttgatcagtt cggcgagtaa gtcgac          956


<210>  10
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human thrombin A / W215A E217A B chains (W215A E217A 
       prethrombin-2), with 14 amino acids added to the N-terminal end

<400>  10

Met Thr Ala Thr Ser Glu Tyr Gln Thr Phe Phe Asp Gly Arg Thr Phe 
1               5                   10                  15      


Gly Ser Gly Glu Ala Asp Cys Gly Leu Arg Pro Leu Phe Glu Lys Lys 
            20                  25                  30          


Ser Leu Glu Asp Lys Thr Glu Arg Glu Leu Leu Glu Ser Tyr Ile Asp 
        35                  40                  45              


Gly Arg Ile Val Glu Gly Ser Asp Ala Glu Ile Gly Met Ser Pro Trp 
    50                  55                  60                  


Gln Val Met Leu Phe Arg Lys Ser Pro Gln Glu Leu Leu Cys Gly Ala 
65                  70                  75                  80  


Ser Leu Ile Ser Asp Arg Trp Val Leu Thr Ala Ala His Cys Leu Leu 
                85                  90                  95      


Tyr Pro Pro Trp Asp Lys Asn Phe Thr Glu Asn Asp Leu Leu Val Arg 
            100                 105                 110         


Ile Gly Lys His Ser Arg Thr Arg Tyr Glu Arg Asn Ile Glu Lys Ile 
        115                 120                 125             


Ser Met Leu Glu Lys Ile Tyr Ile His Pro Arg Tyr Asn Trp Arg Glu 
    130                 135                 140                 


Asn Leu Asp Arg Asp Ile Ala Leu Met Lys Leu Lys Lys Pro Val Ala 
145                 150                 155                 160 


Phe Ser Asp Tyr Ile His Pro Val Cys Leu Pro Asp Arg Glu Thr Ala 
                165                 170                 175     


Ala Ser Leu Leu Gln Ala Gly Tyr Lys Gly Arg Val Thr Gly Trp Gly 
            180                 185                 190         


Asn Leu Lys Glu Thr Trp Thr Ala Asn Val Gly Lys Gly Gln Pro Ser 
        195                 200                 205             


Val Leu Gln Val Val Asn Leu Pro Ile Val Glu Arg Pro Val Cys Lys 
    210                 215                 220                 


Asp Ser Thr Arg Ile Arg Ile Thr Asp Asn Met Phe Cys Ala Gly Tyr 
225                 230                 235                 240 


Lys Pro Asp Glu Gly Lys Arg Gly Asp Ala Cys Glu Gly Asp Ser Gly 
                245                 250                 255     


Gly Pro Phe Val Met Lys Ser Pro Phe Asn Asn Arg Trp Tyr Gln Met 
            260                 265                 270         


Gly Ile Val Ser Ala Gly Ala Gly Cys Asp Arg Asp Gly Lys Tyr Gly 
        275                 280                 285             


Phe Tyr Thr His Val Phe Arg Leu Lys Lys Trp Ile Gln Lys Val Ile 
    290                 295                 300                 


Asp Gln Phe Gly Glu 
305                 


<210>  11
<211>  1310
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized coding sequence for mature ecarin from Echis carinatus 
       (with methionine residue added to the N-terminal end)

<400>  11
gctagcagga ggaattcacc atggtcccgc cacatgaacg caaatttgag aaaaagttta       60

tcgagctggt tgttgttgtt gatcacagca tggtgacgaa atacaataac gacagcaccg      120

cgattcgtac ctggatctac gagatgctga ataccgttaa tgaaatctat ttgccgttta      180

acattagagt ggccctggtg ggtctggagt tttggtgcaa cggtgatctg attaacgtta      240

cgtctaccgc ggatgatacg ctgcatagct ttggtgagtg gcgtgccagc gatctgttga      300

accgcaagcg tcacgaccac gcacagctcc ttaccaacgt cactctggac cactccacgc      360

tgggcatcac cttcgtctac ggcatgtgca agtctgaccg tagcgtcgag ctgattctgg      420

actacagcaa tatcaccttt aacatggcat acattatcgc acacgagatg ggccatagcc      480

tgggtatgct gcacgatacc aagttctgta cgtgcggtgc gaaaccgtgc attatgttcg      540

gcaaagaaag cattccgcct ccaaaagaat ttagctcctg cagctatgat cagtataaca      600

aatacttgct gaaatacaat cctaaatgca tcctggaccc gccgttgcgt aaagacatcg      660

cgagcccggc agtctgcggt aatgaaattt gggaagaggg cgaagagtgt gattgtggta      720

gcccggctga ctgccgcaac ccgtgttgtg atgcggcgac gtgtaagctg aagccgggtg      780

ctgagtgcgg taatggtgag tgctgtgaca aatgcaaaat ccgcaaagcg ggcaccgaat      840

gtcgtccggc acgtgatgat tgcgacgttg cggagcattg taccggtcag agcgcggagt      900

gcccgcgtaa tgaattccaa cgtaacggtc agccgtgcct gaataacagc ggctattgtt      960

acaatggcga ttgcccgatc atgctgaatc agtgcatcgc actgttctcc ccgtcggcta     1020

ctgttgcgca ggacagctgc ttccaacgca acttacaagg tagctactac ggttattgca     1080

ccaaagaaat tggctactat ggtaagcgct tcccgtgtgc cccgcaagac gtgaagtgtg     1140

gtcgtctgta ttgtctggac aattctttca agaagaatat gcgttgtaag aatgactata     1200

gctacgccga cgagaacaag ggcattgtgg aaccgggcac gaagtgcgag gacggtaaag     1260

tgtgcatcaa ccgtaagtgt gtggatgtca acaccgcgta ctaagtcgac                1310


<210>  12
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature ecarin from Echis carinatus (with methionine residue added
       to the N-terminal end)

<400>  12

Met Val Pro Pro His Glu Arg Lys Phe Glu Lys Lys Phe Ile Glu Leu 
1               5                   10                  15      


Val Val Val Val Asp His Ser Met Val Thr Lys Tyr Asn Asn Asp Ser 
            20                  25                  30          


Thr Ala Ile Arg Thr Trp Ile Tyr Glu Met Leu Asn Thr Val Asn Glu 
        35                  40                  45              


Ile Tyr Leu Pro Phe Asn Ile Arg Val Ala Leu Val Gly Leu Glu Phe 
    50                  55                  60                  


Trp Cys Asn Gly Asp Leu Ile Asn Val Thr Ser Thr Ala Asp Asp Thr 
65                  70                  75                  80  


Leu His Ser Phe Gly Glu Trp Arg Ala Ser Asp Leu Leu Asn Arg Lys 
                85                  90                  95      


Arg His Asp His Ala Gln Leu Leu Thr Asn Val Thr Leu Asp His Ser 
            100                 105                 110         


Thr Leu Gly Ile Thr Phe Val Tyr Gly Met Cys Lys Ser Asp Arg Ser 
        115                 120                 125             


Val Glu Leu Ile Leu Asp Tyr Ser Asn Ile Thr Phe Asn Met Ala Tyr 
    130                 135                 140                 


Ile Ile Ala His Glu Met Gly His Ser Leu Gly Met Leu His Asp Thr 
145                 150                 155                 160 


Lys Phe Cys Thr Cys Gly Ala Lys Pro Cys Ile Met Phe Gly Lys Glu 
                165                 170                 175     


Ser Ile Pro Pro Pro Lys Glu Phe Ser Ser Cys Ser Tyr Asp Gln Tyr 
            180                 185                 190         


Asn Lys Tyr Leu Leu Lys Tyr Asn Pro Lys Cys Ile Leu Asp Pro Pro 
        195                 200                 205             


Leu Arg Lys Asp Ile Ala Ser Pro Ala Val Cys Gly Asn Glu Ile Trp 
    210                 215                 220                 


Glu Glu Gly Glu Glu Cys Asp Cys Gly Ser Pro Ala Asp Cys Arg Asn 
225                 230                 235                 240 


Pro Cys Cys Asp Ala Ala Thr Cys Lys Leu Lys Pro Gly Ala Glu Cys 
                245                 250                 255     


Gly Asn Gly Glu Cys Cys Asp Lys Cys Lys Ile Arg Lys Ala Gly Thr 
            260                 265                 270         


Glu Cys Arg Pro Ala Arg Asp Asp Cys Asp Val Ala Glu His Cys Thr 
        275                 280                 285             


Gly Gln Ser Ala Glu Cys Pro Arg Asn Glu Phe Gln Arg Asn Gly Gln 
    290                 295                 300                 


Pro Cys Leu Asn Asn Ser Gly Tyr Cys Tyr Asn Gly Asp Cys Pro Ile 
305                 310                 315                 320 


Met Leu Asn Gln Cys Ile Ala Leu Phe Ser Pro Ser Ala Thr Val Ala 
                325                 330                 335     


Gln Asp Ser Cys Phe Gln Arg Asn Leu Gln Gly Ser Tyr Tyr Gly Tyr 
            340                 345                 350         


Cys Thr Lys Glu Ile Gly Tyr Tyr Gly Lys Arg Phe Pro Cys Ala Pro 
        355                 360                 365             


Gln Asp Val Lys Cys Gly Arg Leu Tyr Cys Leu Asp Asn Ser Phe Lys 
    370                 375                 380                 


Lys Asn Met Arg Cys Lys Asn Asp Tyr Ser Tyr Ala Asp Glu Asn Lys 
385                 390                 395                 400 


Gly Ile Val Glu Pro Gly Thr Lys Cys Glu Asp Gly Lys Val Cys Ile 
                405                 410                 415     


Asn Arg Lys Cys Val Asp Val Asn Thr Ala Tyr 
            420                 425         


<210>  13
<211>  5882
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPRO33 vector

<400>  13
atcgattcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc       60

cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg      120

agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagttgca taaactgcgg      180

cgttaaatcc ggcgtcggtt ccacacttaa aaacagcgcc agtcgttcca tcatattgcg      240

cagttcacga atattgcctg gccagtcgta gtgcagcagc acagtttcac ttgcctgtaa      300

cccctggcgt aatgcagcag aaaatggggc ggagagcgcc gccagagaca ctttcaaaaa      360

gctttccgcc agcggaagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg      420

caaaatactc agccgataaa acagatcacg gcgaaaacgt ccttgctgca tatcttcttc      480

cagattgcag tgagtggcgc taatgacccg tacatctacc ggaacaggct gatgcccgcc      540

gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca aaggtagcgg      600

catttcgcca atctcatcca gaaacagcgt accgccgtgg gcaatttcga acagcccggc      660

gcgacctccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc      720

cagcagcgat tcggcaatcg ccccgcagtt gacggcaaca aacggatgcg actttttgcc      780

ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt      840

gcccgtcccc gtttccccct caatcaacac cgccgcactg gagcgggcat acagcaaaat      900

agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg      960

agtacgcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat     1020

atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt     1080

cattccggct tcttctgcca aatcggtaat cagccctgcg ccgaccaccg cttcggtgcc     1140

gttagctttt agctcgttaa tctgcccgcg tgcgtcttcc tcggtaatgt agctacgttg     1200

gtcgaggcgc aaattaaagg ttttttgaaa cgccaccagc gctggaatgg tttcctgata     1260

ggtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc     1320

gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc     1380

gttagagcca gccgcgatga tggcgtcaca gcgttcgttt gccagtttct tgcggatgta     1440

ggtcactgct ttttcaaagc caagctgaat aggggtaatg ttcgccaggt gatcaaactc     1500

gaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg     1560

tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa     1620

tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa     1680

acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa     1740

ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg     1800

caaataacaa gttgataaca agctagcgaa ttcgagctcg gtacccgggg atcctctaga     1860

gtcgacctgc aggcatgcaa gcttggctgt tttggcggat gagagaagat tttcagcctg     1920

atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt     1980

agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat     2040

ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa     2100

ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct     2160

gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg     2220

gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac     2280

ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata     2340

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga     2400

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc     2460

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg     2520

cagcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg     2580

gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt     2640

tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg     2700

gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat     2760

ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact     2820

gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat     2880

taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag     2940

cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc     3000

aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc     3060

ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt     3120

ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa     3180

caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg     3240

cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat     3300

taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     3360

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     3420

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     3480

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     3540

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     3600

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtc     3660

aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc     3720

aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc     3780

tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt     3840

ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta     3900

ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac     3960

ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa     4020

aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac     4080

ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag     4140

gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc     4200

gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca     4260

agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg     4320

atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt     4380

tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca     4440

ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac     4500

ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga     4560

taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct     4620

tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac     4680

agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc     4740

gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag     4800

gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg     4860

tgcgtaacgg caaaagcacc gccggacatc agcgctagta gcggagtgta tactggctta     4920

ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct     4980

gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac     5040

tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat     5100

ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt     5160

ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt     5220

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc     5280

gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt     5340

ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg     5400

cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     5460

aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga     5520

gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg     5580

cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa     5640

accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg     5700

atctcaagaa gatcatctta ttaatcagat aaaatatttg ctcatgagcc cgaagtggcg     5760

agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc     5820

gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta     5880

tc                                                                    5882


<210>  14
<211>  5977
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPRO33_A expression construct

<400>  14
atcgattcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc       60

cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg      120

agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagttgca taaactgcgg      180

cgttaaatcc ggcgtcggtt ccacacttaa aaacagcgcc agtcgttcca tcatattgcg      240

cagttcacga atattgcctg gccagtcgta gtgcagcagc acagtttcac ttgcctgtaa      300

cccctggcgt aatgcagcag aaaatggggc ggagagcgcc gccagagaca ctttcaaaaa      360

gctttccgcc agcggaagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg      420

caaaatactc agccgataaa acagatcacg gcgaaaacgt ccttgctgca tatcttcttc      480

cagattgcag tgagtggcgc taatgacccg tacatctacc ggaacaggct gatgcccgcc      540

gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca aaggtagcgg      600

catttcgcca atctcatcca gaaacagcgt accgccgtgg gcaatttcga acagcccggc      660

gcgacctccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc      720

cagcagcgat tcggcaatcg ccccgcagtt gacggcaaca aacggatgcg actttttgcc      780

ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt      840

gcccgtcccc gtttccccct caatcaacac cgccgcactg gagcgggcat acagcaaaat      900

agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg      960

agtacgcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat     1020

atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt     1080

cattccggct tcttctgcca aatcggtaat cagccctgcg ccgaccaccg cttcggtgcc     1140

gttagctttt agctcgttaa tctgcccgcg tgcgtcttcc tcggtaatgt agctacgttg     1200

gtcgaggcgc aaattaaagg ttttttgaaa cgccaccagc gctggaatgg tttcctgata     1260

ggtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc     1320

gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc     1380

gttagagcca gccgcgatga tggcgtcaca gcgttcgttt gccagtttct tgcggatgta     1440

ggtcactgct ttttcaaagc caagctgaat aggggtaatg ttcgccaggt gatcaaactc     1500

gaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg     1560

tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa     1620

tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa     1680

acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa     1740

ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg     1800

caaataacaa gttgataaca agctagcagg aggaattcac catgaccttt ggctcgggag     1860

aggcagactg tgggctgcga cctctgttcg agaagaagtc gctggaggac aaaaccgaaa     1920

gagagctcct ggaatcctac atcgacgggc gctaagtcga cctgcaggca tgcaagcttg     1980

gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa     2040

gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca     2100

tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga     2160

gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt     2220

cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg     2280

gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact     2340

gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa     2400

actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc     2460

ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt     2520

cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct     2580

ggtgaaagta aaagatgctg aagatcagtt gggtgcagca aactattaac tggcgaacta     2640

cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga     2700

ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt     2760

gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc     2820

gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct     2880

gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata     2940

ctttagattg atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac     3000

gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc     3060

ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt     3120

agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg     3180

ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac     3240

gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta     3300

ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat     3360

ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt aaaaggatct     3420

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc     3480

actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc     3540

gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg     3600

atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa     3660

atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc     3720

ctacatacct cgctctgcta atcctgttac cagtcaggca tttgagaagc acacggtcac     3780

actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg     3840

accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc     3900

ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa     3960

aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg     4020

ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc     4080

ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata     4140

ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac     4200

atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct     4260

tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa     4320

aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc     4380

agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga     4440

atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc     4500

gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca     4560

aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc     4620

tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt     4680

gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt     4740

cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct     4800

gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc     4860

aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat     4920

cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg     4980

acatcagcgc tagtagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc     5040

agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt     5100

gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc     5160

ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac     5220

ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga     5280

caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag     5340

ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt     5400

taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt     5460

ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc     5520

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac     5580

cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt     5640

taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg     5700

ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg     5760

ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat     5820

cagataaaat atttgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg     5880

tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt     5940

ccggcgtaga ggatctgctc atgtttgaca gcttatc                              5977


<210>  15
<211>  6016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPRO33_A+ expression construct

<400>  15
atcgattcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc       60

cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg      120

agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagttgca taaactgcgg      180

cgttaaatcc ggcgtcggtt ccacacttaa aaacagcgcc agtcgttcca tcatattgcg      240

cagttcacga atattgcctg gccagtcgta gtgcagcagc acagtttcac ttgcctgtaa      300

cccctggcgt aatgcagcag aaaatggggc ggagagcgcc gccagagaca ctttcaaaaa      360

gctttccgcc agcggaagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg      420

caaaatactc agccgataaa acagatcacg gcgaaaacgt ccttgctgca tatcttcttc      480

cagattgcag tgagtggcgc taatgacccg tacatctacc ggaacaggct gatgcccgcc      540

gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca aaggtagcgg      600

catttcgcca atctcatcca gaaacagcgt accgccgtgg gcaatttcga acagcccggc      660

gcgacctccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc      720

cagcagcgat tcggcaatcg ccccgcagtt gacggcaaca aacggatgcg actttttgcc      780

ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt      840

gcccgtcccc gtttccccct caatcaacac cgccgcactg gagcgggcat acagcaaaat      900

agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg      960

agtacgcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat     1020

atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt     1080

cattccggct tcttctgcca aatcggtaat cagccctgcg ccgaccaccg cttcggtgcc     1140

gttagctttt agctcgttaa tctgcccgcg tgcgtcttcc tcggtaatgt agctacgttg     1200

gtcgaggcgc aaattaaagg ttttttgaaa cgccaccagc gctggaatgg tttcctgata     1260

ggtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc     1320

gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc     1380

gttagagcca gccgcgatga tggcgtcaca gcgttcgttt gccagtttct tgcggatgta     1440

ggtcactgct ttttcaaagc caagctgaat aggggtaatg ttcgccaggt gatcaaactc     1500

gaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg     1560

tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa     1620

tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa     1680

acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa     1740

ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg     1800

caaataacaa gttgataaca agctagcagg aggaattcac catgacggca acgagcgagt     1860

accagacctt tttcgacggt agaacctttg gcagcggcga agcggattgc ggtctgcgtc     1920

cgctgttcga gaagaaatcc ctggaagata aaaccgagcg cgagttgctg gaaagctata     1980

tcgacggtcg ttaagtcgac ctgcaggcat gcaagcttgg ctgttttggc ggatgagaga     2040

agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt     2100

tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac     2160

gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat     2220

caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg     2280

gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa     2340

cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag     2400

aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta     2460

aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata     2520

ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc     2580

ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga     2640

agatcagttg ggtgcagcaa actattaact ggcgaactac ttactctagc ttcccggcaa     2700

caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt     2760

ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc     2820

attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg     2880

agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt     2940

aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc     3000

ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact     3060

tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc     3120

cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt     3180

acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc     3240

ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt     3300

gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat     3360

tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa     3420

ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata     3480

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     3540

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     3600

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     3660

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc     3720

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     3780

tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa     3840

accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac     3900

cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg     3960

tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc     4020

cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa     4080

acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat     4140

ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa     4200

ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta     4260

gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac     4320

tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg     4380

aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc     4440

atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa     4500

aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc     4560

tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat     4620

tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct     4680

cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga     4740

aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc     4800

ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca     4860

ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg     4920

atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc     4980

tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct agtagcggag     5040

tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca     5100

ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt     5160

cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg     5220

aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc     5280

cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg     5340

ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     5400

cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt     5460

atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca     5520

agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact     5580

atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta     5640

attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca     5700

agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag     5760

agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg     5820

cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttgctcatg     5880

agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca     5940

accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgctca     6000

tgtttgacag cttatc                                                     6016


<210>  16
<211>  5302
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBAD_WE-B expression construct

<400>  16
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac       60

tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca      120

ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta      180

aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata      240

ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag      300

cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag      360

caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg      420

tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct      480

tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc      540

ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc      600

gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca      660

tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga      720

tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa      780

acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata      840

taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc      900

ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt      960

tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat     1020

tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta     1080

accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt     1140

aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca     1200

ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta     1260

tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca     1320

tgattgttga aggttcggac gcagaaatcg gtatgtcacc atggcaagtt atgttgtttc     1380

gcaaaagccc gcaagagctg ctgtgtggtg cctccctgat ttccgaccgc tgggtcctga     1440

ccgcagcgca ctgtctgctg tatccgccgt gggataagaa tttcaccgag aatgacctgt     1500

tggtgcgcat tggcaaacac agccgtacgc gctacgaacg taacatcgag aagatcagca     1560

tgctggagaa aatctacatt caccctcgtt ataactggcg tgagaacttg gaccgtgaca     1620

tcgctctgat gaagctgaag aaaccggttg cgttcagcga ttacattcat ccggtttgtc     1680

tgccggatcg cgagactgcc gcgagcctgt tacaagccgg ctataagggt cgtgtaacgg     1740

gctggggtaa tctgaaggaa acctggaccg cgaatgtcgg taaaggtcag ccgagcgtgc     1800

tgcaagtggt taatctgccg attgttgaac gtccggtctg caaggatagc acccgcattc     1860

gtatcacgga caacatgttt tgcgcaggct acaagccaga tgaaggcaag cgtggtgatg     1920

cttgcgaggg cgacagcggt ggcccgtttg tgatgaaatc tccgttcaac aaccgttggt     1980

accagatggg tatcgtgagc gcgggtgcgg gttgcgaccg tgacggtaaa tacggcttct     2040

atacccatgt gtttcgcctg aaaaagtgga tccagaaagt cattgatcag ttcggcgagt     2100

aagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc     2160

tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca     2220

gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg     2280

atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga     2340

aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc     2400

ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg     2460

tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg     2520

acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa     2580

tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa     2640

gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct     2700

tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg     2760

tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg     2820

ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt     2880

atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga     2940

cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga     3000

attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac     3060

gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg     3120

ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac     3180

gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct     3240

agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct     3300

gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg     3360

gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat     3420

ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg     3480

tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat     3540

tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     3600

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     3660

tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc     3720

gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta     3780

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta     3840

atagtggact cttgttccaa acttgaacaa cactcaaccc tatctcgggc tattcttttg     3900

atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa     3960

aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat ctaggtgaag     4020

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg     4080

tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc     4140

tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag     4200

ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc     4260

cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac     4320

ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc     4380

gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt     4440

tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt     4500

gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc     4560

ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt     4620

tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca     4680

ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt     4740

tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt     4800

attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag     4860

tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc     4920

ggtatttcac accgcatagg gtcatggctg cgccccgaca cccgccaaca cccgctgacg     4980

cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg     5040

ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcaaggag     5100

atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg     5160

ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg     5220

ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc     5280

tgctcatgtt tgacagctta tc                                              5302


<210>  17
<211>  5449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBAD_A+/WE-B expression construct

<400>  17
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac       60

tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca      120

ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta      180

aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata      240

ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag      300

cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag      360

caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg      420

tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct      480

tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc      540

ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc      600

gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca      660

tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga      720

tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa      780

acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata      840

taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc      900

ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt      960

tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat     1020

tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta     1080

accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt     1140

aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca     1200

ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta     1260

tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca     1320

tgacggcaac tagcgaatac caaacttttt ttgacggtcg taccttcggc agcggcgagg     1380

ctgactgtgg cctgcgccca ctgtttgaaa agaaaagcct ggaagataaa accgagcgtg     1440

agctgctgga gtcttacatc gacggtcgta ttgttgaagg ttcggacgca gaaatcggta     1500

tgtcaccatg gcaagttatg ttgtttcgca aaagcccgca agagctgctg tgtggtgcct     1560

ccctgatttc cgaccgctgg gtcctgaccg cagcgcactg tctgctgtat ccgccgtggg     1620

ataagaattt caccgagaat gacctgttgg tgcgcattgg caaacacagc cgtacgcgct     1680

acgaacgtaa catcgagaag atcagcatgc tggagaaaat ctacattcac cctcgttata     1740

actggcgtga gaacttggac cgtgacatcg ctctgatgaa gctgaagaaa ccggttgcgt     1800

tcagcgatta cattcatccg gtttgtctgc cggatcgcga gactgccgcg agcctgttac     1860

aagccggcta taagggtcgt gtaacgggct ggggtaatct gaaggaaacc tggaccgcga     1920

atgtcggtaa aggtcagccg agcgtgctgc aagtggttaa tctgccgatt gttgaacgtc     1980

cggtctgcaa ggatagcacc cgcattcgta tcacggacaa catgttttgc gcaggctaca     2040

agccagatga aggcaagcgt ggtgatgctt gcgagggcga cagcggtggc ccgtttgtga     2100

tgaaatctcc gttcaacaac cgttggtacc agatgggtat cgtgagcgcg ggtgcgggtt     2160

gcgaccgtga cggtaaatac ggcttctata cccatgtgtt tcgcctgaaa aagtggatcc     2220

agaaagtcat tgatcagttc ggcgagtaag tcgacctgca ggcatgcaag cttggctgtt     2280

ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc     2340

tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga     2400

actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag     2460

ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt     2520

atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg     2580

aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg     2640

catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt     2700

ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata     2760

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct     2820

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa     2880

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa     2940

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt     3000

taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg     3060

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca     3120

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa     3180

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt     3240

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc     3300

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa     3360

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga     3420

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc     3480

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga     3540

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga     3600

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga     3660

ccaagtttac tcatatatac tttagattga tttacgcgcc ctgtagcggc gcattaagcg     3720

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg     3780

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc     3840

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa     3900

aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc     3960

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact tgaacaacac     4020

tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt     4080

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt     4140

ttacaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     4200

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     4260

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     4320

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     4380

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     4440

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     4500

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     4560

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     4620

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     4680

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     4740

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     4800

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     4860

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     4920

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     4980

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     5040

cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggtc atggctgcgc     5100

cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg     5160

cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat     5220

caccgaaacg cgcgaggcag caaggagatg gcgcccaaca gtcccccggc cacggggcct     5280

gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc     5340

ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg     5400

gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatc                 5449


<210>  18
<211>  7147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPRO33_ecarin expression construct

<400>  18
atcgattcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc       60

cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg      120

agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagttgca taaactgcgg      180

cgttaaatcc ggcgtcggtt ccacacttaa aaacagcgcc agtcgttcca tcatattgcg      240

cagttcacga atattgcctg gccagtcgta gtgcagcagc acagtttcac ttgcctgtaa      300

cccctggcgt aatgcagcag aaaatggggc ggagagcgcc gccagagaca ctttcaaaaa      360

gctttccgcc agcggaagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg      420

caaaatactc agccgataaa acagatcacg gcgaaaacgt ccttgctgca tatcttcttc      480

cagattgcag tgagtggcgc taatgacccg tacatctacc ggaacaggct gatgcccgcc      540

gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca aaggtagcgg      600

catttcgcca atctcatcca gaaacagcgt accgccgtgg gcaatttcga acagcccggc      660

gcgacctccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc      720

cagcagcgat tcggcaatcg ccccgcagtt gacggcaaca aacggatgcg actttttgcc      780

ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt      840

gcccgtcccc gtttccccct caatcaacac cgccgcactg gagcgggcat acagcaaaat      900

agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg      960

agtacgcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat     1020

atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt     1080

cattccggct tcttctgcca aatcggtaat cagccctgcg ccgaccaccg cttcggtgcc     1140

gttagctttt agctcgttaa tctgcccgcg tgcgtcttcc tcggtaatgt agctacgttg     1200

gtcgaggcgc aaattaaagg ttttttgaaa cgccaccagc gctggaatgg tttcctgata     1260

ggtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc     1320

gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc     1380

gttagagcca gccgcgatga tggcgtcaca gcgttcgttt gccagtttct tgcggatgta     1440

ggtcactgct ttttcaaagc caagctgaat aggggtaatg ttcgccaggt gatcaaactc     1500

gaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg     1560

tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa     1620

tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa     1680

acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa     1740

ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg     1800

caaataacaa gttgataaca agctagcagg aggaattcac catggtcccg ccacatgaac     1860

gcaaatttga gaaaaagttt atcgagctgg ttgttgttgt tgatcacagc atggtgacga     1920

aatacaataa cgacagcacc gcgattcgta cctggatcta cgagatgctg aataccgtta     1980

atgaaatcta tttgccgttt aacattagag tggccctggt gggtctggag ttttggtgca     2040

acggtgatct gattaacgtt acgtctaccg cggatgatac gctgcatagc tttggtgagt     2100

ggcgtgccag cgatctgttg aaccgcaagc gtcacgacca cgcacagctc cttaccaacg     2160

tcactctgga ccactccacg ctgggcatca ccttcgtcta cggcatgtgc aagtctgacc     2220

gtagcgtcga gctgattctg gactacagca atatcacctt taacatggca tacattatcg     2280

cacacgagat gggccatagc ctgggtatgc tgcacgatac caagttctgt acgtgcggtg     2340

cgaaaccgtg cattatgttc ggcaaagaaa gcattccgcc tccaaaagaa tttagctcct     2400

gcagctatga tcagtataac aaatacttgc tgaaatacaa tcctaaatgc atcctggacc     2460

cgccgttgcg taaagacatc gcgagcccgg cagtctgcgg taatgaaatt tgggaagagg     2520

gcgaagagtg tgattgtggt agcccggctg actgccgcaa cccgtgttgt gatgcggcga     2580

cgtgtaagct gaagccgggt gctgagtgcg gtaatggtga gtgctgtgac aaatgcaaaa     2640

tccgcaaagc gggcaccgaa tgtcgtccgg cacgtgatga ttgcgacgtt gcggagcatt     2700

gtaccggtca gagcgcggag tgcccgcgta atgaattcca acgtaacggt cagccgtgcc     2760

tgaataacag cggctattgt tacaatggcg attgcccgat catgctgaat cagtgcatcg     2820

cactgttctc cccgtcggct actgttgcgc aggacagctg cttccaacgc aacttacaag     2880

gtagctacta cggttattgc accaaagaaa ttggctacta tggtaagcgc ttcccgtgtg     2940

ccccgcaaga cgtgaagtgt ggtcgtctgt attgtctgga caattctttc aagaagaata     3000

tgcgttgtaa gaatgactat agctacgccg acgagaacaa gggcattgtg gaaccgggca     3060

cgaagtgcga ggacggtaaa gtgtgcatca accgtaagtg tgtggatgtc aacaccgcgt     3120

actaagtcga cctgcaggca tgcaagcttg gctgttttgg cggatgagag aagattttca     3180

gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg     3240

gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg     3300

ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa     3360

cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct     3420

ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga     3480

gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc     3540

ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc     3600

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag     3660

gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg     3720

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt     3780

gggtgcagca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     3840

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     3900

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     3960

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     4020

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     4080

taactgtcag accaagttta ctcatatata ctttagattg atttacgcgc cctgtagcgg     4140

cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc     4200

cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc     4260

ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct     4320

cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac     4380

ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac     4440

tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat     4500

ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa     4560

aatattaacg tttacaattt aaaaggatct aggtgaagat cctttttgat aatctcatga     4620

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca     4680

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     4740

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     4800

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     4860

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     4920

cagtcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa     4980

ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa     5040

tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca     5100

ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg     5160

cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga     5220

tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg     5280

gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa     5340

ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag     5400

gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa     5460

tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg     5520

taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat     5580

tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc     5640

ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag     5700

gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata     5760

tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat     5820

ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa     5880

cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta     5940

tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat     6000

tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt     6060

ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg     6120

ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagtagcgga gtgtatactg     6180

gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa     6240

aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca     6300

ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg     6360

gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa     6420

gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca     6480

gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct     6540

cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg     6600

tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact     6660

gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg     6720

agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta     6780

gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt     6840

gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc     6900

gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca     6960

aaacgatctc aagaagatca tcttattaat cagataaaat atttgctcat gagcccgaag     7020

tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct     7080

gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctgctc atgtttgaca     7140

gcttatc                                                               7147


<210>  19
<211>  5883
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPRO43 vector

<400>  19
atcgatttag cttttcagac gacgccaaaa ggtcgtacgt gaaataccca aatagttggc       60

cgcagccgtc ttgtcaccat taaacttctc aagcgcttgc tgcggggtca gcaaacgcgg      120

agccggcgtc tttgcgctct cacgcgccag ctccggcagc agcaactgca tgaattgcgg      180

agtcagatcc ggggtcggct caacggacag gaacagcgcc aggcgttcca tcatattacg      240

cagctcgcgg atgttacccg gccagtcata atgcagcagc accgtttcgc tcgcctgcag      300

accctggcgc agtgccgcag agaacggtgc gctcagggct gccagcgaga ctttcaggaa      360

agactccgcc agcggtaaaa tgtcggcgac acgttcacgc aacggcggga gctgcagacg      420

cagaatgctc aggcggtaga acaggtcgcg acgaaaacgg ccctgttgca tatcctcttc      480

cagattgcag tgggtcgcgc taatcacgcg cacgtctacc ggaaccggtt gatgaccacc      540

gacgcgggtc acttctttct cttccagcac acgcagcaga cgggtttgca atggcagcgg      600

catctcaccg atctcgtcca ggaacaaggt gccgccgtgg gcaatttcaa acaaaccagc      660

acggccaccg cgacggctac ccgtgaatgc gccctcttcg tagccaaaca gctcagcttc      720

cagcaggctt tccgcgattg caccgcaatt aactgccaca aacggatgag atttcttacc      780

ctggcgggca tcgtgacggg cgaaatactc acgatggatt gcttgcgcag ccagttcctt      840

acccgtacca gtctcgcctt cgatcagaac agccgcgctg ctacgtgcat acagcagaat      900

ggtctggcga acttgctcca tttgagggct ttggcccagc atatcaccca ggacataacg      960

ggtacgcagc gcattacgcg tcgcatcgtg ggtgttgtgg cgcaggctca ttctggtcat     1020

gtccagggcg tcgctgaacg cctgacgcac cgttgccgcg ctgtagataa agatgcccgt     1080

catgcccgct tcttcggcca agtccgtgat cagacccgca ccaaccacag cctcggtacc     1140

gttcgctttc agttcgttga tctggccacg tgcatcttcc tcggtaatgt agctgcgttg     1200

gtccaggcgc agattaaagg tcttttgaaa cgcgaccagc gcagggatcg tttcctggta     1260

ggtgacaacg ccaatcgagg aggtcagttt gcctgccttc gccagcgcct gcaagacatc     1320

gtaaccgctc ggcttaatca ggatcaccgg cacggacaga cgggatttca ggtaggcacc     1380

attgctaccc gctgcgataa tggcgtcaca acgctcgttg gccagctttt tgcgaatgta     1440

ggtaacggct ttctcgaaac ccagctgaat cggagtgatg ttcgccaggt gatcaaactc     1500

caggctaatg tcgcggaaca actcgaacag acgggtgacg ctaacggtcc aaataactgg     1560

tttatcatcg ttcaaacgcg gtgggtgtgc catggtgaat acctcctgtt aagaaaccga     1620

atattgggtt taaacttgtt tcataattgt tgcaatgaaa cgcggtgaaa cattgcctga     1680

aacgttaact gaaacgcata tttgcggatt agttcatgac tttatctcta acaaattgaa     1740

attaaacatt taattttatt aaggcaattg tggcacaccc cttgctttgt ctttatcaac     1800

gcaaataaca agttgataac aagctagcga attcgagctc ggtacccggg gatcctctag     1860

agtcgacctg caggcatgca agcttggctg ttttggcgga tgagagaaga ttttcagcct     1920

gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag     1980

tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga     2040

tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa     2100

aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc     2160

tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt     2220

ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga     2280

cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat     2340

atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag     2400

agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt     2460

cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt     2520

gcagcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact     2580

ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt     2640

ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg     2700

ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta     2760

tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac     2820

tgtcagacca agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca     2880

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta     2940

gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt     3000

caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac     3060

cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt     3120

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga     3180

acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg     3240

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata     3300

ttaacgttta caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa     3360

aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg     3420

atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc     3480

gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac     3540

tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca     3600

ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt     3660

caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag     3720

caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg     3780

ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caaccaggcg     3840

tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt     3900

actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa     3960

cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga     4020

aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca     4080

cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca     4140

ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt     4200

cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac     4260

aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg     4320

gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat     4380

ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac     4440

attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa     4500

cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg     4560

ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc     4620

ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa     4680

cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg     4740

cgcaaagtgc gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga     4800

ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg     4860

gtgcgtaacg gcaaaagcac cgccggacat cagcgctagt agcggagtgt atactggctt     4920

actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc     4980

tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga     5040

ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga     5100

tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg     5160

tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg     5220

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg     5280

cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg     5340

tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat     5400

gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     5460

caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg     5520

agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact     5580

gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa     5640

aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac     5700

gatctcaaga agatcatctt attaatcaga taaaatattt gctcatgagc ccgaagtggc     5760

gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg     5820

cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt     5880

atc                                                                   5883


<210>  20
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  J23104 promoter

<400>  20
ttgacagcta gctcagtcct aggtattgtg ctagc                                  35


<210>  21
<211>  6545
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pJ1231-03C plasmid

<400>  21
tcagaattgg ttaattggtt gtaacactga cccctatttg tttatttttc taaatacatt       60

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa      120

ggaagaatat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg      180

atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa      240

tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta      300

gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc      360

cacttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg      420

cgatccccgg aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata      480

ttgttgatgc gctggcagtg ttcctgcgcc ggttgcactc gattcctgtt tgtaattgtc      540

cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg aataacggtt      600

tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga      660

aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct      720

cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag      780

tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt      840

ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata      900

aattgcaatt tcatttgatg ctcgatgagt ttttctaact catgaccaaa atcccttaac      960

gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc     1020

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct     1080

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg     1140

cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt tagcccacca     1200

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc     1260

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga     1320

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac     1380

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga     1440

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag     1500

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg     1560

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag     1620

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc     1680

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc     1740

tcggggtcgt gcaggtagtt tatcattatc aatactcgcc atttcaaaga atacgtaaat     1800

aattaatagt agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc     1860

tgtaacccgt acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt     1920

gtctgggtga acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc     1980

tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca     2040

gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa     2100

cgggcacaac ctcaatggag tgatgcaacc agcctggagt aaatgatgac acaaggcaat     2160

tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct gctctctctg     2220

atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg     2280

actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa     2340

cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac cagaacttag     2400

tttcgacgga taaaatgaca gctttaactg aaggggccaa gcttttcgaa aaagagatcc     2460

catatatcac cgaacttgaa ggtgacgttg agggtatgaa gtttatcata aaaggagaag     2520

gcacaggtga tgcgacaacg ggtacaatca aggcaaagta catttgtaca actggggacc     2580

tgcctgtccc atgggccact ttggtgtcta ctttgtctta cggcgtacaa tgttttgcta     2640

agtacccttc acacatcaaa gatttcttta agtctgcaat gcctgaagga tacacacagg     2700

aacgtacaat ttcatttgag ggcgacggtg tctataaaac aagagctatg gttacttatg     2760

aaagaggttc catctacaac agagtgacac taacgggcga aaatttcaaa aaggatggac     2820

atattttgcg taaaaacgta gctttccaat gcccaccatc aatactatac attctgccag     2880

atactgtaaa caatggtatt agagtcgagt ttaatcaagc ttatgatata gaaggtgtca     2940

ctgaaaaatt ggttacaaaa tgcagccaaa tgaatagacc attggcagga tctgccgctg     3000

tgcatatccc tagataccat cacattacct accacaccaa attaagtaaa gacagggatg     3060

aacgaagaga tcatatgtgt ttagttgagg ttgttaaggc agttgatctc gacacttacc     3120

aataaatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc     3180

taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag     3240

ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac     3300

gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga     3360

aggctttaat ttgcggcccc tcacctgcac gcaaaatagg ataattatac tctatttctc     3420

aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga aatatcttga     3480

ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc     3540

aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca     3600

aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt     3660

caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag     3720

aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat     3780

ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc     3840

ttacctttta catttcagca atatatatat atatatttca aggatatacc attctaatgt     3900

ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg     3960

aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg     4020

aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg     4080

cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat     4140

ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat     4200

tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa     4260

tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta     4320

tttactttgg taagagaaag gaagatgatg gtgatggtgt cgcttgggat agtgaacaat     4380

acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg     4440

agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat     4500

ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc     4560

aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta     4620

taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt     4680

ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg     4740

gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta     4800

tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag     4860

gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggcatcaga actggtgatt     4920

taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa     4980

tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa     5040

ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt aacgctatga     5100

tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca     5160

gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg     5220

gataatatca caggaggtac tagactacct ttcatcctac ataaatagac gcatataagt     5280

acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca     5340

acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt     5400

ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct     5460

agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa gtcgcacttt     5520

caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga ataccgcttc     5580

cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat ccctatataa     5640

cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct acatttttta     5700

tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta ttcatagagt     5760

gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag agacaaaata     5820

gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc actttctgtt     5880

cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct ttatcttgaa     5940

aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt caggcttttt     6000

ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg acctacagtg     6060

caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa gtaatctaag     6120

atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa aaagaagtat     6180

agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta aaaatgcagc     6240

tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt acaaaaatga     6300

agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt ctgtaaaaat     6360

gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttctacaaa     6420

atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta     6480

tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat     6540

attgg                                                                 6545


<210>  22
<211>  6009
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pJ1234-03C plasmid

<400>  22
tcagaattgg ttaattggtt gtaacactga cccctatttg tttatttttc taaatacatt       60

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa      120

ggaagaatat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt      180

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt      240

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt      300

ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg      360

tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga      420

atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa      480

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga      540

caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa      600

ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca      660

ccacgatgcc tgtagcgatg gcaacaacgt tgcgcaaact attaactggc gaactactta      720

ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac      780

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatccgga gccggtgagc      840

gtggttctcg cggtatcatc gcagcgctgg ggccagatgg taagccctcc cgtatcgtag      900

ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga      960

taggtgcctc actgattaag cattggtaac tcatgaccaa aatcccttaa cgtgagttac     1020

gcgcgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga     1080

tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt     1140

ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag     1200

agcgcagata ccaaatactg ttcttctagt gtagccgtag ttagcccacc acttcaagaa     1260

ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag     1320

tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca     1380

gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac     1440

cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa     1500

ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc     1560

agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg     1620

tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc     1680

ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc     1740

ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcggggtcg     1800

tgcaggtagt ttatcattat caatactcgc catttcaaag aatacgtaaa taattaatag     1860

tagtgatttt cctaacttta tttagtcaaa aaattagcct tttaattctg ctgtaacccg     1920

tacatgccca aaataggggg cgggttacac agaatatata acatcgtagg tgtctgggtg     1980

aacagtttat tcctggcatc cactaaatat aatggagccc gctttttaag ctggcatcca     2040

gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc accaaccatc agttcatagg     2100

tccattctct tagcgcaact acagagaaca ggggcacaaa caggcaaaaa acgggcacaa     2160

cctcaatgga gtgatgcaac cagcctggag taaatgatga cacaaggcaa ttgacccacg     2220

catgtatcta tctcattttc ttacaccttc tattaccttc tgctctctct gatttggaaa     2280

aagctgaaaa aaaaggttga aaccagttcc ctgaaattat tcccctactt gactaataag     2340

tatataaaga cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa     2400

ttctactttt atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg     2460

ataaaatgac agctttaact gaaggggcca agcttttcga aaaagagatc ccatatatca     2520

ccgaacttga aggtgacgtt gagggtatga agtttatcat aaaaggagaa ggcacaggtg     2580

atgcgacaac gggtacaatc aaggcaaagt acatttgtac aactggggac ctgcctgtcc     2640

catgggccac tttggtgtct actttgtctt acggcgtaca atgttttgct aagtaccctt     2700

cacacatcaa agatttcttt aagtctgcaa tgcctgaagg atacacacag gaacgtacaa     2760

tttcatttga gggcgacggt gtctataaaa caagagctat ggttacttat gaaagaggtt     2820

ccatctacaa cagagtgaca ctaacgggcg aaaatttcaa aaaggatgga catattttgc     2880

gtaaaaacgt agctttccaa tgcccaccat caatactata cattctgcca gatactgtaa     2940

acaatggtat tagagtcgag tttaatcaag cttatgatat agaaggtgtc actgaaaaat     3000

tggttacaaa atgcagccaa atgaatagac cattggcagg atctgccgct gtgcatatcc     3060

ctagatacca tcacattacc taccacacca aattaagtaa agacagggat gaacgaagag     3120

atcatatgtg tttagttgag gttgttaagg cagttgatct cgacacttac caataaatca     3180

tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa     3240

aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag     3300

tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg     3360

catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa     3420

tttgcggccc ctcacctgca cgcaaaaagc ttttcaattc aattcatcat ttttttttta     3480

ttcttttttt tgatttcggt ttctttgaaa tttttttgat tcggtaatct ccgaacagaa     3540

ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga     3600

agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa ccagcaggaa     3660

acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc     3720

tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt     3780

ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg     3840

tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc     3900

gctaaaggca ttatccgcca agtacaattt tttactcttc gaagatagaa aatttgctga     3960

cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc     4020

agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc     4080

ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa     4140

gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga aaagcgacaa     4200

agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga     4260

ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagatgcat tgggtcaaca     4320

gtatagaacc gtggatgatg ttgtctctac aggatctgac attattattg ttggaagagg     4380

actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg     4440

ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat aagtaaatgc     4500

atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat tacccacgct     4560

atgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt     4620

ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga     4680

atgggataat atcacaggag gtactagact acctttcatc ctacataaat agacgcatat     4740

aagtacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca     4800

ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg     4860

cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt     4920

ctctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagtcgca     4980

ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg     5040

cttccacaaa cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat     5100

ataacctacc catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt     5160

tttatgttta tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata     5220

gagtgaatcg aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa     5280

aatagaagaa accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc     5340

tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct     5400

tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct     5460

ttttttatgg aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac     5520

agtgcaaaaa gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc     5580

taagatgctt tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa     5640

gtatagattc tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg     5700

cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa     5760

atgaagcaca gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa     5820

aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta     5880

caaaatgaag cacagatgct tcgttcaggt ggcacttttc ggggaaatgt gcgcggaacc     5940

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     6000

tgatattgg                                                             6009


