               SEQUENCE LISTING

<110> MEDIMMUNE LIMITED

<120> Amino acid derivatives

<130> ALLO-280WO1

<150> US61/862,497
<151> 2013-08-05

<160> 17

<170> BiSSAP 1.3

<210> 1
<211> 1365
<212> DNA
<213> Methanosarcina mazei

<220> 
<223> PylRS Methanosarcina mazei WT nucleotide sequence

<400> 1
atggataaaa aaccactaaa cactctgata tctgcaaccg ggctctggat gtccaggacc      60

ggaacaattc ataaaataaa acaccacgaa gtctctcgaa gcaaaatcta tattgaaatg     120

gcatgcggag accaccttgt tgtaaacaac tccaggagca gcaggactgc aagagcgctc     180

aggcaccaca aatacaggaa gacctgcaaa cgctgcaggg tttcggatga ggatctcaat     240

aagttcctca caaaggcaaa cgaagaccag acaagcgtaa aagtcaaggt cgtttctgcc     300

cctaccagaa cgaaaaaggc aatgccaaaa tccgttgcga gagccccgaa acctcttgag     360

aatacagaag cggcacaggc tcaaccttct ggatctaaat tttcacctgc gataccggtt     420

tccacccaag agtcagtttc tgtcccggca tctgtttcaa catcaatatc aagcatttct     480

acaggagcaa ctgcatccgc actggtaaaa gggaatacga accccattac atccatgtct     540

gcccctgttc aggcaagtgc ccccgcactt acgaagagcc agactgacag gcttgaagtc     600

ctgttaaacc caaaagatga gatttccctg aattccggca agcctttcag ggagcttgag     660

tccgaattgc tctctcgcag aaaaaaagac ctgcagcaga tctacgcgga agaaagggag     720

aattatctgg ggaaactcga gcgtgaaatt accaggttct ttgtggacag gggttttctg     780

gaaataaaat ccccgatcct gatccctctt gagtatatcg aaaggatggg cattgataat     840

gataccgaac tttcaaaaca gatcttcagg gttgacaaga acttctgcct gagacccatg     900

cttgctccaa acctttacaa ctacctgcgc aagcttgaca gggccctgcc tgatccaata     960

aaaatttttg aaataggccc atgctacaga aaagagtccg acggcaaaga acacctcgaa    1020

gagtttacca tgctgaactt ctgccagatg ggatcgggat gcacacggga aaatcttgaa    1080

agcataatta cggacttcct gaaccacctg ggaattgatt tcaagatcgt aggcgattcc    1140

tgcatggtct atggggatac ccttgatgta atgcacggag acctggaact ttcctctgca    1200

gtagtcggac ccataccgct tgaccgggaa tggggtattg ataaaccctg gataggggca    1260

ggtttcgggc tcgaacgcct tctaaaggtt aaacacgact ttaaaaatat caagagagct    1320

gcaaggtccg agtcttacta taacgggatt tctaccaacc tgtaa                    1365


<210> 2
<211> 454
<212> PRT
<213> Methanosarcina mazei

<220> 
<223> PylRS, Methanosarcina mazei WT amino acid sequence

<400> 2
Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      
Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          
Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              
Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  
Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  
Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      
Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         
Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             
Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 
Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 
Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     
Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         
Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             
Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 
Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 
Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     
Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         
Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             
Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 
Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 
Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     
Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         
Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             
His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 
Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 
Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     
Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         
Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             
Gly Ile Ser Thr Asn Leu 
    450                 

<210> 3
<211> 1365
<212> DNA
<213> Artificial Sequence

<220> 
<223> PylRS Methanosarcina mazei Y384F mutant nucleotide sequence

<400> 3
atggataaaa aaccactaaa cactctgata tctgcaaccg ggctctggat gtccaggacc      60

ggaacaattc ataaaataaa acaccacgaa gtctctcgaa gcaaaatcta tattgaaatg     120

gcatgcggag accaccttgt tgtaaacaac tccaggagca gcaggactgc aagagcgctc     180

aggcaccaca aatacaggaa gacctgcaaa cgctgcaggg tttcggatga ggatctcaat     240

aagttcctca caaaggcaaa cgaagaccag acaagcgtaa aagtcaaggt cgtttctgcc     300

cctaccagaa cgaaaaaggc aatgccaaaa tccgttgcga gagccccgaa acctcttgag     360

aatacagaag cggcacaggc tcaaccttct ggatctaaat tttcacctgc gataccggtt     420

tccacccaag agtcagtttc tgtcccggca tctgtttcaa catcaatatc aagcatttct     480

acaggagcaa ctgcatccgc actggtaaaa gggaatacga accccattac atccatgtct     540

gcccctgttc aggcaagtgc ccccgcactt acgaagagcc agactgacag gcttgaagtc     600

ctgttaaacc caaaagatga gatttccctg aattccggca agcctttcag ggagcttgag     660

tccgaattgc tctctcgcag aaaaaaagac ctgcagcaga tctacgcgga agaaagggag     720

aattatctgg ggaaactcga gcgtgaaatt accaggttct ttgtggacag gggttttctg     780

gaaataaaat ccccgatcct gatccctctt gagtatatcg aaaggatggg cattgataat     840

gataccgaac tttcaaaaca gatcttcagg gttgacaaga acttctgcct gagacccatg     900

cttgctccaa acctttacaa ctacctgcgc aagcttgaca gggccctgcc tgatccaata     960

aaaatttttg aaataggccc atgctacaga aaagagtccg acggcaaaga acacctcgaa    1020

gagtttacca tgctgaactt ctgccagatg ggatcgggat gcacacggga aaatcttgaa    1080

agcataatta cggacttcct gaaccacctg ggaattgatt tcaagatcgt aggcgattcc    1140

tgcatggtct ttggggatac ccttgatgta atgcacggag acctggaact ttcctctgca    1200

gtagtcggac ccataccgct tgaccgggaa tggggtattg ataaaccctg gataggggca    1260

ggtttcgggc tcgaacgcct tctaaaggtt aaacacgact ttaaaaatat caagagagct    1320

gcaaggtccg agtcttacta taacgggatt tctaccaacc tgtaa                    1365


<210> 4
<211> 454
<212> PRT
<213> Artificial Sequence

<220> 
<223> PylRS Methanosarcina mazei Y384F mutant amino acid sequence

<400> 4
Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      
Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          
Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              
Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  
Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  
Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      
Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         
Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             
Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 
Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 
Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     
Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         
Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             
Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 
Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 
Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     
Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         
Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             
Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 
Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 
Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     
Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         
Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             
His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Phe 
    370                 375                 380                 
Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 
Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     
Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         
Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             
Gly Ile Ser Thr Asn Leu 
    450                 

<210> 5
<211> 72
<212> DNA
<213> Methanosarcina mazei

<220> 
<223> tRNApyl Methanosarcina mazei Go1

<400> 5
ggaaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt tagattcccg     60

gggtttccgc ca                                                         72


<210> 6
<211> 250
<212> DNA
<213> Unknown

<220> 
<223> U6 snRNA promoter

<400> 6
agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga     60

gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag    120

aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca    180

tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg    240

acgaaacacc                                                           250


<210> 7
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> U6-tRNApyl construct

<400> 7
aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac      60

aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa     120

aatacgtgac gtagaaagta ataatttctt gggtagtttg cagtttttaa aattatgttt     180

taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata     240

tatcttgtgg aaaggacgaa acaccgaatt ctctagactc gagggaaacc tgatcatgta     300

gatcgaatgg actctaaatc cgttcagccg ggttagattc ccggggtttc cggacaagtg     360

cggtttttgt tt                                                         372


<210> 8
<211> 1104
<212> DNA
<213> Unknown

<220> 
<223> GFP nuclelotide sequence

<400> 8
atggcctcca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat      60

ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac     120

ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca     180

cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa     240

cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct     300

ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt     360

gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac     420

aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat     480

ggaatcaaag ctaacttcaa aattcgtcac aacattgaag atggatccgt tcaactagca     540

gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat     600

tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc     660

cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatcaggc caagcctttg     720

tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc     780

tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt     840

gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct     900

gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc     960

atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa    1020

gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc    1080

tctggttatg tgtgggaggg ctaa                                           1104


<210> 9
<211> 367
<212> PRT
<213> Unknown

<220> 
<223> GFP amino acid sequence

<400> 9
Met Ala Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Phe Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Gln Ala Lys Pro Leu 
225                 230                 235                 240 
Ser Gln Glu Glu Ser Thr Leu Ile Glu Arg Ala Thr Ala Thr Ile Asn 
                245                 250                 255     
Ser Ile Pro Ile Ser Glu Asp Tyr Ser Val Ala Ser Ala Ala Leu Ser 
            260                 265                 270         
Ser Asp Gly Arg Ile Phe Thr Gly Val Asn Val Tyr His Phe Thr Gly 
        275                 280                 285             
Gly Pro Cys Ala Glu Leu Val Val Leu Gly Thr Ala Ala Ala Ala Ala 
    290                 295                 300                 
Ala Gly Asn Leu Thr Cys Ile Val Ala Ile Gly Asn Glu Asn Arg Gly 
305                 310                 315                 320 
Ile Leu Ser Pro Cys Gly Arg Cys Arg Gln Val Leu Leu Asp Leu His 
                325                 330                 335     
Pro Gly Ile Lys Ala Ile Val Lys Asp Ser Asp Gly Gln Pro Thr Ala 
            340                 345                 350         
Val Gly Ile Arg Glu Leu Leu Pro Ser Gly Tyr Val Trp Glu Gly 
        355                 360                 365         

<210> 10
<211> 1104
<212> DNA
<213> Artificial Sequence

<220> 
<223> GFPY40 nucleotide sequence

<400> 10
atggcctcca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat      60

ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatag     120

ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca     180

cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa     240

cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct     300

ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt     360

gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac     420

aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat     480

ggaatcaaag ctaacttcaa aattcgtcac aacattgaag atggatccgt tcaactagca     540

gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat     600

tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc     660

cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatcaggc caagcctttg     720

tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc     780

tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt     840

gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct     900

gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc     960

atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa    1020

gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc    1080

tctggttatg tgtgggaggg ctaa                                           1104


<210> 11
<211> 367
<212> PRT
<213> Artificial Sequence

<220> 
<223> GFPY40 amino acid sequence

<220> 
<221> MUTAGEN
<222> 40
<223> Xaa = unspecified nnAA

<400> 11
Met Ala Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Xaa Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Phe Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Gln Ala Lys Pro Leu 
225                 230                 235                 240 
Ser Gln Glu Glu Ser Thr Leu Ile Glu Arg Ala Thr Ala Thr Ile Asn 
                245                 250                 255     
Ser Ile Pro Ile Ser Glu Asp Tyr Ser Val Ala Ser Ala Ala Leu Ser 
            260                 265                 270         
Ser Asp Gly Arg Ile Phe Thr Gly Val Asn Val Tyr His Phe Thr Gly 
        275                 280                 285             
Gly Pro Cys Ala Glu Leu Val Val Leu Gly Thr Ala Ala Ala Ala Ala 
    290                 295                 300                 
Ala Gly Asn Leu Thr Cys Ile Val Ala Ile Gly Asn Glu Asn Arg Gly 
305                 310                 315                 320 
Ile Leu Ser Pro Cys Gly Arg Cys Arg Gln Val Leu Leu Asp Leu His 
                325                 330                 335     
Pro Gly Ile Lys Ala Ile Val Lys Asp Ser Asp Gly Gln Pro Thr Ala 
            340                 345                 350         
Val Gly Ile Arg Glu Leu Leu Pro Ser Gly Tyr Val Trp Glu Gly 
        355                 360                 365         

<210> 12
<211> 1413
<212> DNA
<213> Artificial Sequence

<220> 
<223> anti-Her2 (4D5) gamma nucleotide sequence

<400> 12
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt      60

gaggtgcagc tggtggagtc tggcggtggc ttggtacagc cgggcgggtc cctgcgcctc     120

tcctgtgccg cttccggatt caacatcaaa gacacgtata ttcactgggt ccgtcaggca     180

cctggcaagg gtctggagtg ggtgagccgc atttatccta ccaatggtta cactcgctac     240

gccgactctg tgaagggccg cttcaccatc agcgccgaca cgtccaagaa caccctgtat     300

ctgcaaatga acagcctgcg tgccgaggac accgcggtgt attactgcag ccgctggggc     360

ggtgatggct tttacgcgat ggactactgg ggccagggca ccctggtcac cgtctcgagt     420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg     480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg     540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca     600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc     660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc     720

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga     780

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct     840

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg     900

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac     960

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag    1020

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc    1080

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag    1140

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc    1200

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg    1260

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg    1320

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg    1380

cagaagagcc tctccctgtc tccgggtaaa tga                                 1413


<210> 13
<211> 470
<212> PRT
<213> Artificial Sequence

<220> 
<223> anti-Her2 (4D5) gamma amino acid sequence

<400> 13
Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      
Asp Thr Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          
Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              
Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  
Leu Glu Trp Val Ser Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  
Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      
Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         
Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             
Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 
Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 
Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     
Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         
Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             
Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 
Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 
Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 
Ser Leu Ser Pro Gly Lys 
465                 470 

<210> 14
<211> 1413
<212> DNA
<213> Artificial Sequence

<220> 
<223> anti-Her2 (4D5) gamma_K274amber nucleotide sequence

<400> 14
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt      60

gaggtgcagc tggtggagtc tggcggtggc ttggtacagc cgggcgggtc cctgcgcctc     120

tcctgtgccg cttccggatt caacatcaaa gacacgtata ttcactgggt ccgtcaggca     180

cctggcaagg gtctggagtg ggtgagccgc atttatccta ccaatggtta cactcgctac     240

gccgactctg tgaagggccg cttcaccatc agcgccgaca cgtccaagaa caccctgtat     300

ctgcaaatga acagcctgcg tgccgaggac accgcggtgt attactgcag ccgctggggc     360

ggtgatggct tttacgcgat ggactactgg ggccagggca ccctggtcac cgtctcgagt     420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg     480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg     540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca     600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc     660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc     720

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga     780

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct     840

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcta gttcaactgg     900

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac     960

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag    1020

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc    1080

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag    1140

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc    1200

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg    1260

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg    1320

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg    1380

cagaagagcc tctccctgtc tccgggtaaa tga                                 1413


<210> 15
<211> 470
<212> PRT
<213> Artificial Sequence

<220> 
<223> anti-Her2 (4D5) gamma_K274amber amino acid sequence

<220> 
<221> MUTAGEN
<222> 297
<223> Xaa = pyrrolysine analogue of formula III.1 or 1A.1

<400> 15
Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      
Asp Thr Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          
Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              
Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  
Leu Glu Trp Val Ser Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  
Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      
Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         
Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             
Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 
Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 
Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     
Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         
Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             
Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 
Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 
Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             
Val Ser His Glu Asp Pro Glu Val Xaa Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 
Ser Leu Ser Pro Gly Lys 
465                 470 

<210> 16
<211> 705
<212> DNA
<213> Artificial Sequence

<220> 
<223> anti-Her2 (4D5) Kappa nucleotide sequence

<400> 16
atggaggctc ccgcccagct gctctttctg ctccttctct ggcttcccga cacaaccggt      60

gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtgggaga ccgtgtcaca     120

atcacttgcc gtgctagcca ggatgtgaat acagcggtgg cctggtatca gcagaaacct     180

ggcaaagccc ctaagctcct gatctattct gcatcctttt tgtacagcgg cgtgccgagc     240

cgcttcagcg gcagccgttc tggtaccgat ttcactctca ccatcagctc tctgcaaccg     300

gaagattttg caacttacta ctgtcaacag cactacacca ctcctccgac gttcggccaa     360

gggaccaagg tggaaatcga acgtacggtg gctgcaccat ctgtcttcat cttcccgcca     420

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat     480

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag     540

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg     600

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc     660

ctgagttcgc ccgtcacaaa gagcttcaac aggggagagt gttaa                     705


<210> 17
<211> 234
<212> PRT
<213> Artificial Sequence

<220> 
<223> anti-Her2 (4D5) Kappa amino acid sequence

<400> 17
Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      
Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          
Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
        35                  40                  45              
Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  
Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
65                  70                  75                  80  
Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      
Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
            100                 105                 110         
Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Glu Arg 
        115                 120                 125             
Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 
Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 
Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     
Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         
Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             
His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 
Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 

