                         SEQUENCE LISTING

<110>  CEA
 
<120>  LIGASE E3 RNF185 INHIBITORS AND USES THEREOF

<130>  B1661PC

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  1
gauauuugcc acagcauuu                                                    19


<210>  2
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  2
cuucuguugg ccguguuua                                                    19


<210>  3
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  3
gaagatctgc aagcaagggg ccctcggcc                                         29


<210>  4
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  4
ccgctcgagt taggcaatca ggagccagaa catg                                   34


<210>  5
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  5
ccgctcgagt tagcgtgaca ggaactgctc gtc                                    33


<210>  6
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  6
gaagatctag gggcagcact gggcaac                                           27


<210>  7
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  7
cgaggccaac atcgccttgg acacagccaa ggatgcc                                37


<210>  8
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  8
ccaaggcgat gttggcctcg aaagtgctgt cctgcc                                 36


<210>  9
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  9
uagcaaugac gaaugcgua                                                    19


<210>  10
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  10
tcttctgttg gccgtgttta ca                                                22


<210>  11
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  11
ttgcagactg gacacacttg tc                                                22


<210>  12
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  12
atggggaagg tgaaggtcg                                                    19


<210>  13
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  13
ggggtcattg atggcaacaa ta                                                22


<210>  14
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  14
cacagtggtg cctaccaaga                                                   20


<210>  15
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  15
tgtcttttgt caggggtctt t                                                 21


<210>  16
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  16
cgccgctaga ggtgaaattc                                                   20


<210>  17
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  17
ttggcaaatg ctttcgctc                                                    19


<210>  18
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  18
atggcaaatg ctggaccaaa                                                   20


<210>  19
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  19
gccttctttc accttcccaa a                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  20
ccaaagauug cuuucacaau u                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  21
gguggagucu ucuuucucau u                                                 21


<210>  22
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  22
caguaauggc agcauuugu                                                    19


<210>  23
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  23
gaucacagug gucgccugc                                                    19


<210>  24
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  24
ccaccgucuu caaugcccat t                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  25
cggcaagagu guccaguaut t                                                 21


<210>  26
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  26
cucacucagu aacguuguut t                                                 21


<210>  27
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  27
cugcucagag gcucacucat t                                                 21


<210>  28
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  28
gatccccagc tgggatcagc agagagttca agagactctc tgctgatccc agcttttttg       60

gaaa                                                                    64


<210>  29
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  29
agcttttcca aaaaagctgg gatcagcaga gagtctcttg aactctctgc tgatcccagc       60

tggg                                                                    64


<210>  30
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  30
gatcccgcgc gaccttcgaa tgtaattcaa gagattacat tcgaaggtcg cgcttttttg       60

gaaa                                                                    64


<210>  31
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  31
agcttttcca aaaaagcgcg accttcgaat gtaatctctt gaattacatt cgaaggtcgc       60

gcgg                                                                    64


<210>  32
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  32
gatcccgaga aggttgtccc gctttattca agagataaag cgggacaacc ttcttttttg       60

gaaa                                                                    64


<210>  33
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  33
agcttttcca aaaaagaagg ttgtcccgct ttatctcttg aataaagcgg gacaaccttc       60

tcgg                                                                    64


<210>  34
<211>  65
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  34
gatcccgcgc gaccttcgaa tgtaatttca agagaattac attcgaaggt cgcgtttttt       60

ggaaa                                                                   65


<210>  35
<211>  65
<212>  DNA
<213>  artificial sequence

<220>
<223>  shRNA

<400>  35
agcttttcca aaaaacgcga ccttcgaatg taattctctt gaaattacat tcgaaggtcg       60

cgcgg                                                                   65


<210>  36
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  36
agaccagacc uaacagacat t                                                 21


<210>  37
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  37
caucaguggu uggagaccat t                                                 21


<210>  38
<211>  180
<212>  PRT
<213>  artificial sequence

<220>
<223>  RNF5 Mus musculus

<400>  38

Met Ala Ala Ala Glu Glu Glu Asp Gly Gly Pro Glu Gly Pro Asn Arg 
1               5                   10                  15      


Glu Arg Gly Gly Ala Ser Ala Thr Phe Glu Cys Asn Ile Cys Leu Glu 
            20                  25                  30          


Thr Ala Arg Glu Ala Val Val Ser Val Cys Gly His Leu Tyr Cys Trp 
        35                  40                  45              


Pro Cys Leu His Gln Trp Leu Glu Thr Arg Pro Asp Arg Gln Glu Cys 
    50                  55                  60                  


Pro Val Cys Lys Ala Gly Ile Ser Arg Glu Lys Val Val Pro Leu Tyr 
65                  70                  75                  80  


Gly Arg Gly Ser Gln Lys Pro Gln Asp Pro Arg Leu Lys Thr Pro Pro 
                85                  90                  95      


Arg Pro Gln Gly Gln Arg Pro Ala Pro Glu Ser Arg Gly Gly Phe Gln 
            100                 105                 110         


Pro Phe Gly Asp Ala Gly Gly Phe His Phe Ser Phe Gly Val Gly Ala 
        115                 120                 125             


Phe Pro Phe Gly Phe Phe Thr Thr Val Phe Asn Ala His Glu Pro Phe 
    130                 135                 140                 


Arg Arg Gly Ala Gly Val Asp Leu Gly Gln Gly His Pro Ala Ser Ser 
145                 150                 155                 160 


Trp Gln Asp Ser Leu Phe Leu Phe Leu Ala Ile Phe Phe Phe Phe Trp 
                165                 170                 175     


Leu Leu Ser Ile 
            180 


<210>  39
<211>  180
<212>  PRT
<213>  artificial sequence

<220>
<223>  RNF5 Homo sapiens

<400>  39

Met Ala Ala Ala Glu Glu Glu Asp Gly Gly Pro Glu Gly Pro Asn Arg 
1               5                   10                  15      


Glu Arg Gly Gly Ala Gly Ala Thr Phe Glu Cys Asn Ile Cys Leu Glu 
            20                  25                  30          


Thr Ala Arg Glu Ala Val Val Ser Val Cys Gly His Leu Tyr Cys Trp 
        35                  40                  45              


Pro Cys Leu His Gln Trp Leu Glu Thr Arg Pro Glu Arg Gln Glu Cys 
    50                  55                  60                  


Pro Val Cys Lys Ala Gly Ile Ser Arg Glu Lys Val Val Pro Leu Tyr 
65                  70                  75                  80  


Gly Arg Gly Ser Gln Lys Pro Gln Asp Pro Arg Leu Lys Thr Pro Pro 
                85                  90                  95      


Arg Pro Gln Gly Gln Arg Pro Ala Pro Glu Ser Arg Gly Gly Phe Gln 
            100                 105                 110         


Pro Phe Gly Asp Thr Gly Gly Phe His Phe Ser Phe Gly Val Gly Ala 
        115                 120                 125             


Phe Pro Phe Gly Phe Phe Thr Thr Val Phe Asn Ala His Glu Pro Phe 
    130                 135                 140                 


Arg Arg Gly Thr Gly Val Asp Leu Gly Gln Gly His Pro Ala Ser Ser 
145                 150                 155                 160 


Trp Gln Asp Ser Leu Phe Leu Phe Leu Ala Ile Phe Phe Phe Phe Trp 
                165                 170                 175     


Leu Leu Ser Ile 
            180 


<210>  40
<211>  235
<212>  PRT
<213>  artificial sequence

<220>
<223>  RNF5 Caenorhabditis elegans

<400>  40

Met Ala Ser Glu Thr Lys Ala Pro Ser Glu Glu Pro Thr Ser Ser Ser 
1               5                   10                  15      


Asn Lys Asp Glu Ser Ala Arg Phe Glu Cys Asn Ile Cys Leu Asp Ala 
            20                  25                  30          


Ala Lys Asp Ala Val Val Ser Leu Cys Gly His Leu Phe Cys Trp Pro 
        35                  40                  45              


Cys Leu Ser Gln Trp Leu Asp Thr Arg Pro Asn Asn Gln Val Cys Pro 
    50                  55                  60                  


Val Cys Lys Ser Ala Ile Asp Gly Asn Lys Val Val Pro Ile Tyr Gly 
65                  70                  75                  80  


Arg Gly Gly Asp Ser Ser Asp Pro Arg Lys Lys Val Pro Pro Arg Pro 
                85                  90                  95      


Lys Gly Gln Arg Ser Glu Pro Pro Pro Gln Ser Phe Ala Gly Phe Asn 
            100                 105                 110         


Trp Gly Gly Asp Gly Gly Met Met Gly Gly Gly Gly Pro Asn Val His 
        115                 120                 125             


Phe Ser Phe Gly Ile Gly Thr Val Asn Gly Leu Phe Pro Leu Met Phe 
    130                 135                 140                 


Met Leu Pro Phe Ile Gln Gly Ile Phe Pro Leu Ser Phe Val Ala Ser 
145                 150                 155                 160 


Phe Phe Gly Asn Gly Asn Gln Gly Ala Ala Ala Ala Gly Gly Gly Asn 
                165                 170                 175     


Gly Gly Gly Asn Asp Gly Asn Asp Gly Thr His Ala His Thr His Gly 
            180                 185                 190         


His Thr His Gly Pro Arg Gly His Gly Glu Ser Ala Ala Pro Gly Ser 
        195                 200                 205             


Arg Met Ala Gln Glu Glu Glu Tyr Leu Ser Asn Ile Phe Lys Tyr Ile 
    210                 215                 220                 


Gly Phe Phe Met Leu Phe Trp Leu Leu Phe Val 
225                 230                 235 


<210>  41
<211>  192
<212>  PRT
<213>  artificial sequence

<220>
<223>  RNF185 Homo sapiens

<400>  41

Met Ala Ser Lys Gly Pro Ser Ala Ser Ala Ser Pro Glu Asn Ser Ser 
1               5                   10                  15      


Ala Gly Gly Pro Ser Gly Ser Ser Asn Gly Ala Gly Glu Ser Gly Gly 
            20                  25                  30          


Gln Asp Ser Thr Phe Glu Cys Asn Ile Cys Leu Asp Thr Ala Lys Asp 
        35                  40                  45              


Ala Val Ile Ser Leu Cys Gly His Leu Phe Cys Trp Pro Cys Leu His 
    50                  55                  60                  


Gln Trp Leu Glu Thr Arg Pro Asn Arg Gln Val Cys Pro Val Cys Lys 
65                  70                  75                  80  


Ala Gly Ile Ser Arg Asp Lys Val Ile Pro Leu Tyr Gly Arg Gly Ser 
                85                  90                  95      


Thr Gly Gln Gln Asp Pro Arg Glu Lys Thr Pro Pro Arg Pro Gln Gly 
            100                 105                 110         


Gln Arg Pro Glu Pro Glu Asn Arg Gly Gly Phe Gln Gly Phe Gly Phe 
        115                 120                 125             


Gly Asp Gly Gly Phe Gln Met Ser Phe Gly Ile Gly Ala Phe Pro Phe 
    130                 135                 140                 


Gly Ile Phe Ala Thr Ala Phe Asn Ile Asn Asp Gly Arg Pro Pro Pro 
145                 150                 155                 160 


Ala Val Pro Gly Thr Pro Gln Tyr Val Asp Glu Gln Phe Leu Ser Arg 
                165                 170                 175     


Leu Phe Leu Phe Val Ala Leu Val Ile Met Phe Trp Leu Leu Ile Ala 
            180                 185                 190         


<210>  42
<211>  192
<212>  PRT
<213>  artificial sequence

<220>
<223>  RNF185 Mus musculus

<400>  42

Met Ala Ser Lys Gly Pro Ser Ala Ser Ala Ser Thr Glu Asn Ser Asn 
1               5                   10                  15      


Ala Gly Gly Pro Ser Gly Ser Ser Asn Gly Thr Gly Glu Ser Gly Gly 
            20                  25                  30          


Gln Asp Ser Thr Phe Glu Cys Asn Ile Cys Leu Asp Thr Ala Lys Asp 
        35                  40                  45              


Ala Val Ile Ser Leu Cys Gly His Leu Phe Cys Trp Pro Cys Leu His 
    50                  55                  60                  


Gln Trp Leu Glu Thr Arg Pro Asn Arg Gln Val Cys Pro Val Cys Lys 
65                  70                  75                  80  


Ala Gly Ile Ser Arg Asp Lys Val Ile Pro Leu Tyr Gly Arg Gly Ser 
                85                  90                  95      


Thr Gly Gln Gln Asp Pro Arg Glu Lys Thr Pro Pro Arg Pro Gln Gly 
            100                 105                 110         


Gln Arg Pro Glu Pro Glu Asn Arg Gly Gly Phe Gln Gly Phe Gly Phe 
        115                 120                 125             


Gly Asp Gly Gly Phe Gln Met Ser Phe Gly Ile Gly Ala Phe Pro Phe 
    130                 135                 140                 


Gly Ile Phe Ala Thr Ala Phe Asn Ile Asn Asp Gly Arg Pro Pro Pro 
145                 150                 155                 160 


Ala Val Pro Gly Thr Pro Gln Tyr Val Asp Glu Gln Phe Leu Ser Arg 
                165                 170                 175     


Leu Phe Leu Phe Val Ala Leu Val Ile Met Phe Trp Leu Leu Ile Ala 
            180                 185                 190         


<210>  43
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  43
cagacuuugu cauggagcut t                                                 21


<210>  44
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  siRNA

<400>  44
gucauguggu gccucuggut t                                                 21


