                         SEQUENCE LISTING

<110>  St Vincent's Institute of Medical Research
 
<120>  An antibody therapy for amyloid beta disease

<130>  35221550/EJH

<150>  AU 2013902922
<151>  2013-08-05

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  215
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of heavy chain of Fab 2286 antibody

<400>  1

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser 
        115                 120                 125             


Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr 
                165                 170                 175     


Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr 
            180                 185                 190         


Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
        195                 200                 205             


Lys Ile Val Pro Arg Asp Cys 
    210                 215 


<210>  2
<211>  215
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of heavy chain of Fab 2286-like antibody

<400>  2

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser 
        115                 120                 125             


Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr 
                165                 170                 175     


Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr 
            180                 185                 190         


Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
        195                 200                 205             


Lys Ile Val Pro Arg Asp Cys 
    210                 215 


<210>  3
<211>  214
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of light chain (hkappa)

<400>  3

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Phe Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Arg Lys Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  4
<211>  28
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of Abeta1-28

<400>  4

Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys 
1               5                   10                  15      


Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys 
            20                  25              


<210>  5
<211>  40
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of Abeta1-40

<400>  5

Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys 
1               5                   10                  15      


Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala Ile Ile 
            20                  25                  30          


Gly Leu Met Val Gly Gly Val Val 
        35                  40  


<210>  6
<211>  42
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of Abeta1-42

<400>  6

Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys 
1               5                   10                  15      


Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala Ile Ile 
            20                  25                  30          


Gly Leu Met Val Gly Gly Val Val Ile Ala 
        35                  40          


<210>  7
<211>  43
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of Abeta1-43

<400>  7

Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys 
1               5                   10                  15      


Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala Ile Ile 
            20                  25                  30          


Gly Leu Met Val Gly Gly Val Val Ile Ala Thr 
        35                  40              


<210>  8
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of Abeta35-40

<400>  8

Met Val Gly Gly Val Val 
1               5       


<210>  9
<211>  437
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of murinized Fab 2286-like antibody heavy 
       chain conjugated to human gamma-1 immunoglobulin (hG1) hevy 
       constant region with Glu50Arg substitution

<400>  9

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser 
        115                 120                 125             


Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr 
                165                 170                 175     


Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr 
            180                 185                 190         


Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
        195                 200                 205             


Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val 
    210                 215                 220                 


Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val 
225                 230                 235                 240 


Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile 
                245                 250                 255     


Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val 
            260                 265                 270         


Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser 
        275                 280                 285             


Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu 
    290                 295                 300                 


Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala 
305                 310                 315                 320 


Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro 
                325                 330                 335     


Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys 
            340                 345                 350         


Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr 
        355                 360                 365             


Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr 
    370                 375                 380                 


Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu 
385                 390                 395                 400 


Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser 
                405                 410                 415     


Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser 
            420                 425                 430         


His Ser Pro Gly Lys 
        435         


<210>  10
<211>  216
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of chimeric Fab2286 antibody heavy chain 
       variable region

<400>  10

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             


Lys Lys Val Glu Pro Lys Ser Cys 
    210                 215     


<210>  11
<211>  222
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid seuqnece of chimeric Fab 2286-like antibody heavy 
       chain variable region

<400>  11

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             


Lys Lys Val Glu Pro Lys Ser Cys His His His His His His 
    210                 215                 220         


<210>  12
<211>  645
<212>  DNA
<213>  artificial

<220>
<223>  Monoclonal antibody 2286 nucleic acid seuqence: heavy chain

<400>  12
gaggtgaagc ttctcgagtc tggaggtggc ctggtgcagc ctggaggatc cctgaaactc       60

tcctgtgcag cctcaggatt cgattttagt agatactgga tgaattgggt ccggcaggct      120

ccagggaaag ggctagaatg gattggagaa attaatccag atagcagtac gataaactat      180

acgccatctc taaaggataa attcatcatc tccagagaca acgccaaaaa tacgctgtac      240

ctgcaaatga gcaaagtgag atctgaggac acagcccttt attactgtgc aagacaaatg      300

ggctactggg gccaaggcac cactctcaca gtctcctcag ccaaaacgac acccccatct      360

gtctatccac tggcccctgg atctgctgcc caaactaact ccatggtgac cctgggatgc      420

ctggtcaagg gctatttccc tgagccagtg acagtgacct ggaactctgg atccctgtcc      480

agcggtgtgc acaccttccc agctgtcctg cagtctgacc tctacactct gagcagctca      540

gtgactgtcc cctccagcac ctggcccagc gagaccgtca cctgcaacgt tgcccacccg      600

gccagcagca ccaaggtgga caagaaaatt gtgcccaggg attgt                      645


<210>  13
<211>  215
<212>  PRT
<213>  artificial

<220>
<223>  Mab2286 amino acid sequence: heavy chain

<400>  13

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser 
        115                 120                 125             


Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr 
                165                 170                 175     


Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr 
            180                 185                 190         


Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
        195                 200                 205             


Lys Ile Val Pro Arg Asp Cys 
    210                 215 


<210>  14
<211>  642
<212>  DNA
<213>  artificial

<220>
<223>  Monoclonal antibody 2286 nucleic acid seuqence: light chain

<400>  14
gatatccaga tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc       60

atcagttgca gtgcaagtca gggcattagc aattatttaa actggtttca gcagaaacca      120

gatggaactg ttaaactcct gatctattac acatcaagtt tacactcagg agtcccatca      180

aggttcagtg gcagtgggtc tgggacagat tattctctca ccatcagcaa cctggaacct      240

gaagatattg ccacttacta ttgtcagcag tataggaagc ttccgtacac gttcggaggg      300

gggaccaagc tggaaataaa acgggctgat gctgcaccaa ctgtatccat cttcccacca      360

tccagtgagc agttaacatc tggaggtgcc tcagtcgtgt gcttcttgaa caacttctac      420

cccaaagaca tcaatgtcaa gtggaagatt gatggcagtg aacgacaaaa tggcgtcctg      480

aacagttgga ctgatcagga cagcaaagac agcacctaca gcatgagcag caccctcacg      540

ttgaccaagg acgagtatga acgacataac agctatacct gtgaggccac tcacaagaca      600

tcaacttcac ccattgtcaa gagcttcaac aggaatgagt gt                         642


<210>  15
<211>  214
<212>  PRT
<213>  artificial

<220>
<223>  Mab 2886 amino acid sequence: light chain

<400>  15

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Phe Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Arg Lys Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly 
        115                 120                 125             


Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile 
    130                 135                 140                 


Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu 
145                 150                 155                 160 


Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr 
            180                 185                 190         


Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser 
        195                 200                 205             


Phe Asn Arg Asn Glu Cys 
    210                 


<210>  16
<211>  1311
<212>  DNA
<213>  artificial

<220>
<223>  Murine heavy A_Opt, Length: 1391 bp

<400>  16
gaggtcaaac tgctggagag tggaggggga ctggtgcagc caggcgggtc actgaagctg       60

agctgcgccg cttccggctt cgacttttcc cggtactgga tgaattgggt gagacaggct      120

cccggaaaag gcctggagtg gatcggggaa attaatcctg atagctccac catcaactac      180

acaccaagtc tgaaggacaa attcatcatt tcacgcgata acgcaaagaa tactctgtat      240

ctgcagatgt ctaaagtgcg aagtgaggac accgcactgt actattgtgc aagacagatg      300

ggatactggg gacagggaac cacactgacc gtgtctagtg ctaagactac ccctcccagc      360

gtgtatcctc tggcacctgg ctccgcagca cagaccaatt ctatggtgac actgggctgt      420

ctggtcaagg ggtacttccc tgagccagtc acagtgactt ggaacagcgg cagcctgtca      480

agcggcgtgc acacctttcc tgccgtcctg cagagcgatc tgtatacact gtcctctagt      540

gtcactgtgc cctcaagcac ctggccttcc gagaccgtga catgcaacgt cgcccatcct      600

gcttcctcta caaaggtgga caagaaaatc gtcccacgag attgcggctg taaaccatgc      660

atttgtactg tccccgaagt gagttcagtc ttcatctttc cacccaagcc aaaagacgtg      720

ctgactatta ccctgacacc caaggtcaca tgcgtggtcg tggatatcag caaagacgat      780

cccgaggtgc agttctcctg gtttgtcgac gatgtcgaag tgcacacagc ccagactcag      840

cccagggagg aacagttcaa ttctaccttt cgctctgtga gtgagctgcc tattatgcat      900

caggactggc tgaatggaaa ggaattcaaa tgcagagtga actctgctgc atttcccgct      960

cctatcgaga agactattag caagaccaaa ggcaggccta aagccccaca ggtgtacaca     1020

atccctccac ccaaggaaca gatggctaag gataaagtga gcctgacatg tatgatcact     1080

gacttctttc ccgaggatat taccgtggaa tggcagtgga acgggcagcc cgcagagaac     1140

tataagaata cacagcctat tatggacact gatggatcat acttcgtgta tagcaagctg     1200

aacgtccaga aatctaattg ggaagccggc aacactttta cctgtagtgt gctgcatgaa     1260

ggactgcata accatcatac tgaaaagtca ctgtctcatt caccaggcaa a              1311


<210>  17
<211>  437
<212>  PRT
<213>  artificial

<220>
<223>  Murine heavy A_Opt amino acid sequence

<400>  17

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser 
        115                 120                 125             


Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr 
                165                 170                 175     


Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr 
            180                 185                 190         


Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
        195                 200                 205             


Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val 
    210                 215                 220                 


Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val 
225                 230                 235                 240 


Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile 
                245                 250                 255     


Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val 
            260                 265                 270         


Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser 
        275                 280                 285             


Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu 
    290                 295                 300                 


Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala 
305                 310                 315                 320 


Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro 
                325                 330                 335     


Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys 
            340                 345                 350         


Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr 
        355                 360                 365             


Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr 
    370                 375                 380                 


Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu 
385                 390                 395                 400 


Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser 
                405                 410                 415     


Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser 
            420                 425                 430         


His Ser Pro Gly Lys 
        435         


<210>  18
<211>  1311
<212>  DNA
<213>  artificial

<220>
<223>  Murine heavy B, variant sequence

<400>  18
gaggtcaaac tgctggagag tggaggggga ctggtgcagc caggcgggtc actgaagctg       60

agctgcgccg cttccggctt cgacttttcc cggtactgga tgaattgggt gagacaggct      120

cccggaaaag gcctggagtg gatcgggcgt attaatcctg atagctccac catcaactac      180

acaccaagtc tgaaggacaa attcatcatt tcacgcgata acgcaaagaa tactctgtat      240

ctgcagatgt ctaaagtgcg aagtgaggac accgcactgt actattgtgc aagacagatg      300

ggatactggg gacagggaac cacactgacc gtgtctagtg ctaagactac ccctcccagc      360

gtgtatcctc tggcacctgg ctccgcagca cagaccaatt ctatggtgac actgggctgt      420

ctggtcaagg ggtacttccc tgagccagtc acagtgactt ggaacagcgg cagcctgtca      480

agcggcgtgc acacctttcc tgccgtcctg cagagcgatc tgtatacact gtcctctagt      540

gtcactgtgc cctcaagcac ctggccttcc gagaccgtga catgcaacgt cgcccatcct      600

gcttcctcta caaaggtgga caagaaaatc gtcccacgag attgcggctg taaaccatgc      660

atttgtactg tccccgaagt gagttcagtc ttcatctttc cacccaagcc aaaagacgtg      720

ctgactatta ccctgacacc caaggtcaca tgcgtggtcg tggatatcag caaagacgat      780

cccgaggtgc agttctcctg gtttgtcgac gatgtcgaag tgcacacagc ccagactcag      840

cccagggagg aacagttcaa ttctaccttt cgctctgtga gtgagctgcc tattatgcat      900

caggactggc tgaatggaaa ggaattcaaa tgcagagtga actctgctgc atttcccgct      960

cctatcgaga agactattag caagaccaaa ggcaggccta aagccccaca ggtgtacaca     1020

atccctccac ccaaggaaca gatggctaag gataaagtga gcctgacatg tatgatcact     1080

gacttctttc ccgaggatat taccgtggaa tggcagtgga acgggcagcc cgcagagaac     1140

tataagaata cacagcctat tatggacact gatggatcat acttcgtgta tagcaagctg     1200

aacgtccaga aatctaattg ggaagccggc aacactttta cctgtagtgt gctgcatgaa     1260

ggactgcata accatcatac tgaaaagtca ctgtctcatt caccaggcaa a              1311


<210>  19
<211>  437
<212>  PRT
<213>  artificial

<220>
<223>  Murine heavy B amino acid sequence

<400>  19

Glu Val Lys Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu 
    50                  55                  60                  


Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Lys Val Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Met Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser 
        115                 120                 125             


Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr 
                165                 170                 175     


Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr 
            180                 185                 190         


Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
        195                 200                 205             


Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val 
    210                 215                 220                 


Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val 
225                 230                 235                 240 


Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile 
                245                 250                 255     


Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val 
            260                 265                 270         


Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser 
        275                 280                 285             


Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu 
    290                 295                 300                 


Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala 
305                 310                 315                 320 


Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro 
                325                 330                 335     


Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys 
            340                 345                 350         


Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr 
        355                 360                 365             


Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr 
    370                 375                 380                 


Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu 
385                 390                 395                 400 


Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser 
                405                 410                 415     


Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser 
            420                 425                 430         


His Ser Pro Gly Lys 
        435         


<210>  20
<211>  642
<212>  DNA
<213>  artificial

<220>
<223>  Murine light_Opt length: 725bp

<400>  20
gatattcaga tgactcagac tacttcttcc ctgtctgcaa gtctggggga ccgagtgaca       60

atctcatgca gcgcctccca gggaatttcc aactacctga attggttcca gcagaagcct      120

gatggcacag tgaaactgct gatctactat actagctccc tgcacagtgg ggtcccatca      180

agattttctg gaagtggctc agggaccgac tatagcctga caatctccaa cctggagcca      240

gaagatattg ccacttacta ttgccagcag taccggaagc tgccctatac tttcggcggg      300

ggaaccaagc tggagatcaa aagagctgac gccgctccca ccgtgagcat ttttccccct      360

tctagtgaac agctgacctc tggcggggca agtgtggtct gtttcctgaa caacttctac      420

cctaaagaca tcaacgtgaa gtggaaaatt gatggaagcg agaggcagaa cggcgtcctg      480

aattcctgga ccgaccagga tagcaaggac tccacatatt ctatgtcaag caccctgaca      540

ctgactaaag atgagtacga acgccataat agctatacat gtgaagctac tcataagacc      600

tctacctctc ctattgtgaa atcttttaac cgaaatgaat gt                         642


<210>  21
<211>  214
<212>  PRT
<213>  artificial

<220>
<223>  Murine light_Opt amino acid sequence

<400>  21

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Phe Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Arg Lys Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly 
        115                 120                 125             


Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile 
    130                 135                 140                 


Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu 
145                 150                 155                 160 


Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr 
            180                 185                 190         


Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser 
        195                 200                 205             


Phe Asn Arg Asn Glu Cys 
    210                 


