                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Jarjour, Jordan
       Astrakhan, Alexander
       Certo, Michael
 
<120>  MULTIPARTITE SIGNALING PROTEINS AND USES THEREOF

<130>  BLBD-036/02WO

<150>  US 61/934,092
<151>  2014-01-31

<150>  US 61/859,697
<151>  2013-07-29

<160>  75    

<170>  PatentIn version 3.5

<210>  1
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFvCD19-DmrA protein

<400>  1

Met Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala 
1               5                   10                  15      


Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile 
            20                  25                  30          


Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys 
        35                  40                  45              


Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg 
    50                  55                  60                  


Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn 
65                  70                  75                  80  


Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr 
                85                  90                  95      


Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser 
            100                 105                 110         


Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly 
        115                 120                 125             


Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
    130                 135                 140                 


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
145                 150                 155                 160 


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
                165                 170                 175     


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
            180                 185                 190         


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
        195                 200                 205             


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
    210                 215                 220                 


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Ser Val Thr Val Ser Ser Ala Ser Gly Gly Gly Gly Ser Gly 
                245                 250                 255     


Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys 
            260                 265                 270         


Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly 
        275                 280                 285             


Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met 
    290                 295                 300                 


Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln 
305                 310                 315                 320 


Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala 
                325                 330                 335     


Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu 
            340                 345                 350         


Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        355                 360             


<210>  2
<211>  1149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA mRNA

<400>  2
augccccugg gccugcugug gcugggccug gcccugcugg gcgcccugca cgcccaggcc       60

ggauccgaua uccagaugac ccagaccacc agcagccuga gcgccagccu gggcgauaga      120

gugaccauca gcugcagagc cagccaggac aucagcaagu accugaacug guaucagcag      180

aaacccgacg gcaccgugaa gcugcugauc uaccacacca gcagacugca cagcggcgug      240

cccagcagau uuucuggcag cggcuccggc accgacuaca gccugaccau cuccaaccug      300

gaacaggaag auaucgcuac cuacuucugu cagcaaggca acacccugcc cuacaccuuc      360

ggcggaggca ccaagcugga aaucaccggc agcacaagcg gcagcggcaa gccuggaucu      420

ggcgagggaa gcaccaaggg cgaagugaaa cugcaggaaa gcggcccugg acugguggcc      480

ccaagccagu cucugagcgu gaccuguacc guguccggcg ugucccugcc ugacuauggc      540

guguccugga ucagacagcc ccccagaaag ggccuggaau ggcugggagu gaucuggggc      600

agcgagacaa ccuacuacaa cagcgcccug aagucccggc ugaccaucau caaggacaac      660

uccaagagcc agguguuccu gaagaugaac agccugcaga ccgacgacac cgccaucuac      720

uacugcgcca agcacuacua cuacggcggc agcuacgcca uggacuacug gggccagggc      780

acaagcguga ccguguccag cgcuagcggc ggagguggga gcggagugca gguggaaacc      840

aucuccccag gagacgggcg caccuucccc aagcgcggcc agaccugcgu ggugcacuac      900

accgggaugc uugaagaugg aaagaaauuu gauuccuccc gggacagaaa caagcccuuu      960

aaguuuaugc uaggcaagca ggaggugauc cgaggcuggg aagaaggggu ugcccagaug     1020

aguguggguc agagagccaa acugacuaua ucuccagauu augccuaugg ugccacuggg     1080

cacccaggca ucaucccacc acaugccacu cucgucuucg auguggagcu ucuaaaacug     1140

gaaggcuga                                                             1149


<210>  3
<211>  1149
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA DNA

<400>  3
atgcccctgg gcctgctgtg gctgggcctg gccctgctgg gcgccctgca cgcccaggcc       60

ggatccgata tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgataga      120

gtgaccatca gctgcagagc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

cccagcagat tttctggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgctac ctacttctgt cagcaaggca acaccctgcc ctacaccttc      360

ggcggaggca ccaagctgga aatcaccggc agcacaagcg gcagcggcaa gcctggatct      420

ggcgagggaa gcaccaaggg cgaagtgaaa ctgcaggaaa gcggccctgg actggtggcc      480

ccaagccagt ctctgagcgt gacctgtacc gtgtccggcg tgtccctgcc tgactatggc      540

gtgtcctgga tcagacagcc ccccagaaag ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

acaagcgtga ccgtgtccag cgctagcggc ggaggtggga gcggagtgca ggtggaaacc      840

atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac      900

accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt      960

aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg     1020

agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg     1080

cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg     1140

gaaggctga                                                             1149


<210>  4
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFvCD19-DmrB protein

<400>  4

Met Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala 
1               5                   10                  15      


Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile 
            20                  25                  30          


Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys 
        35                  40                  45              


Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg 
    50                  55                  60                  


Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn 
65                  70                  75                  80  


Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr 
                85                  90                  95      


Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser 
            100                 105                 110         


Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly 
        115                 120                 125             


Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
    130                 135                 140                 


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
145                 150                 155                 160 


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
                165                 170                 175     


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
            180                 185                 190         


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
        195                 200                 205             


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
    210                 215                 220                 


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Ser Val Thr Val Ser Ser Ala Ser Gly Gly Gly Gly Ser Gly 
                245                 250                 255     


Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys 
            260                 265                 270         


Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly 
        275                 280                 285             


Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met 
    290                 295                 300                 


Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln 
305                 310                 315                 320 


Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala 
                325                 330                 335     


Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu 
            340                 345                 350         


Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        355                 360             


<210>  5
<211>  1149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrB mRNA

<400>  5
augccccugg gccugcugug gcugggccug gcccugcugg gcgcccugca cgcccaggcc       60

ggauccgaua uccagaugac ccagaccacc agcagccuga gcgccagccu gggcgauaga      120

gugaccauca gcugcagagc cagccaggac aucagcaagu accugaacug guaucagcag      180

aaacccgacg gcaccgugaa gcugcugauc uaccacacca gcagacugca cagcggcgug      240

cccagcagau uuucuggcag cggcuccggc accgacuaca gccugaccau cuccaaccug      300

gaacaggaag auaucgcuac cuacuucugu cagcaaggca acacccugcc cuacaccuuc      360

ggcggaggca ccaagcugga aaucaccggc agcacaagcg gcagcggcaa gccuggaucu      420

ggcgagggaa gcaccaaggg cgaagugaaa cugcaggaaa gcggcccugg acugguggcc      480

ccaagccagu cucugagcgu gaccuguacc guguccggcg ugucccugcc ugacuauggc      540

guguccugga ucagacagcc ccccagaaag ggccuggaau ggcugggagu gaucuggggc      600

agcgagacaa ccuacuacaa cagcgcccug aagucccggc ugaccaucau caaggacaac      660

uccaagagcc agguguuccu gaagaugaac agccugcaga ccgacgacac cgccaucuac      720

uacugcgcca agcacuacua cuacggcggc agcuacgcca uggacuacug gggccagggc      780

acaagcguga ccguguccag cgcuagcggc ggagguggga gcggagugca gguggaaacc      840

aucuccccag gagacgggcg caccuucccc aagcgcggcc agaccugcgu ggugcacuac      900

accgggaugc uugaagaugg aaagaaaguu gauuccuccc gggacagaaa caagcccuuu      960

aaguuuaugc uaggcaagca ggaggugauc cgaggcuggg aagaaggggu ugcccagaug     1020

aguguggguc agagagccaa acugacuaua ucuccagauu augccuaugg ugccacuggg     1080

cacccaggca ucaucccacc acaugccacu cucgucuucg auguggagcu ucuaaaacug     1140

gaaggcuga                                                             1149


<210>  6
<211>  1149
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrB DNA

<400>  6
atgcccctgg gcctgctgtg gctgggcctg gccctgctgg gcgccctgca cgcccaggcc       60

ggatccgata tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgataga      120

gtgaccatca gctgcagagc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

cccagcagat tttctggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgctac ctacttctgt cagcaaggca acaccctgcc ctacaccttc      360

ggcggaggca ccaagctgga aatcaccggc agcacaagcg gcagcggcaa gcctggatct      420

ggcgagggaa gcaccaaggg cgaagtgaaa ctgcaggaaa gcggccctgg actggtggcc      480

ccaagccagt ctctgagcgt gacctgtacc gtgtccggcg tgtccctgcc tgactatggc      540

gtgtcctgga tcagacagcc ccccagaaag ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

acaagcgtga ccgtgtccag cgctagcggc ggaggtggga gcggagtgca ggtggaaacc      840

atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac      900

accgggatgc ttgaagatgg aaagaaagtt gattcctccc gggacagaaa caagcccttt      960

aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg     1020

agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg     1080

cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg     1140

gaaggctga                                                             1149


<210>  7
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFvCD19-DmrC protein

<400>  7

Met Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala 
1               5                   10                  15      


Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile 
            20                  25                  30          


Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys 
        35                  40                  45              


Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg 
    50                  55                  60                  


Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn 
65                  70                  75                  80  


Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr 
                85                  90                  95      


Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser 
            100                 105                 110         


Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly 
        115                 120                 125             


Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
    130                 135                 140                 


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
145                 150                 155                 160 


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
                165                 170                 175     


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
            180                 185                 190         


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
        195                 200                 205             


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
    210                 215                 220                 


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Ser Val Thr Val Ser Ser Ala Ser Gly Gly Gly Gly Ser Ile 
                245                 250                 255     


Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu 
            260                 265                 270         


Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro 
        275                 280                 285             


Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser 
    290                 295                 300                 


Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys 
305                 310                 315                 320 


Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp 
                325                 330                 335     


Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys Gly 
            340                 345                 


<210>  8
<211>  1107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrC mRNA

<400>  8
augccccugg gccugcugug gcugggccug gcccugcugg gcgcccugca cgcccaggcc       60

ggauccgaua uccagaugac ccagaccacc agcagccuga gcgccagccu gggcgauaga      120

gugaccauca gcugcagagc cagccaggac aucagcaagu accugaacug guaucagcag      180

aaacccgacg gcaccgugaa gcugcugauc uaccacacca gcagacugca cagcggcgug      240

cccagcagau uuucuggcag cggcuccggc accgacuaca gccugaccau cuccaaccug      300

gaacaggaag auaucgcuac cuacuucugu cagcaaggca acacccugcc cuacaccuuc      360

ggcggaggca ccaagcugga aaucaccggc agcacaagcg gcagcggcaa gccuggaucu      420

ggcgagggaa gcaccaaggg cgaagugaaa cugcaggaaa gcggcccugg acugguggcc      480

ccaagccagu cucugagcgu gaccuguacc guguccggcg ugucccugcc ugacuauggc      540

guguccugga ucagacagcc ccccagaaag ggccuggaau ggcugggagu gaucuggggc      600

agcgagacaa ccuacuacaa cagcgcccug aagucccggc ugaccaucau caaggacaac      660

uccaagagcc agguguuccu gaagaugaac agccugcaga ccgacgacac cgccaucuac      720

uacugcgcca agcacuacua cuacggcggc agcuacgcca uggacuacug gggccagggc      780

acaagcguga ccguguccag cgcuagcggc ggagguggga gcauccucug gcaugagaug      840

uggcaugaag gccuggaaga ggcaucucgu uuguacuuug gggaaaggaa cgugaaaggc      900

auguuugagg ugcuggagcc cuugcaugcu augauggaac ggggccccca gacucugaag      960

gaaacauccu uuaaucaggc cuauggucga gauuuaaugg aggcccaaga guggugcagg     1020

aaguacauga aaucagggaa ugucaaggac cuccuccaag ccugggaccu cuauuaucau     1080

guguuccgac gaaucucaaa gggcuga                                         1107


<210>  9
<211>  1107
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrC DNA

<400>  9
atgcccctgg gcctgctgtg gctgggcctg gccctgctgg gcgccctgca cgcccaggcc       60

ggatccgata tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgataga      120

gtgaccatca gctgcagagc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

cccagcagat tttctggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgctac ctacttctgt cagcaaggca acaccctgcc ctacaccttc      360

ggcggaggca ccaagctgga aatcaccggc agcacaagcg gcagcggcaa gcctggatct      420

ggcgagggaa gcaccaaggg cgaagtgaaa ctgcaggaaa gcggccctgg actggtggcc      480

ccaagccagt ctctgagcgt gacctgtacc gtgtccggcg tgtccctgcc tgactatggc      540

gtgtcctgga tcagacagcc ccccagaaag ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

acaagcgtga ccgtgtccag cgctagcggc ggaggtggga gcatcctctg gcatgagatg      840

tggcatgaag gcctggaaga ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc      900

atgtttgagg tgctggagcc cttgcatgct atgatggaac ggggccccca gactctgaag      960

gaaacatcct ttaatcaggc ctatggtcga gatttaatgg aggcccaaga gtggtgcagg     1020

aagtacatga aatcagggaa tgtcaaggac ctcctccaag cctgggacct ctattatcat     1080

gtgttccgac gaatctcaaa gggctga                                         1107


<210>  10
<211>  7255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_scFvCD19-DmrA-BFP lentiviral vector

<400>  10
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccccccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcggag tgcaggtgga aaccatctcc ccaggagacg     3120

ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag     3180

atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca     3240

agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag     3300

ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc     3360

caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc ggccgctccg     3420

gtgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccg ggcccctcta     3480

gaagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca     3540

accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca     3600

tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta     3660

gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca     3720

agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg     3780

tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga     3840

tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg     3900

aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg     3960

ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca     4020

agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa     4080

gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat     4140

actgcgacct ccctagcaaa ctggggcaca agcttaattg agtcgaccga gcatcttacc     4200

gccatttata cccatatttg ttctgttttt cttgatttgg gtatacattt aaatgttaat     4260

agaacaaaat ggtggggcaa tcatttacat ttttagggat atgtaattac tagttcaggt     4320

gtattgccac aagacaaaca tgttaagaaa ctttcccgtt atttacgctc tgttcctgtt     4380

aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct     4440

ccttttacgc tgtgtggata tgctgcttta tagcctctgt atctagctat tgcttcccgt     4500

acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctctttt agaggagttg     4560

tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact     4620

ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg     4680

atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg     4740

ctgggcactg ataattccgt ggtgttgtca tcgaattcgg taccttttta aaagaaaagg     4800

ggggactgga agggctaatt cactcccaac gaagacaaga tatcataact tcgtatagca     4860

tacattatac gaagttataa tttatttgtg aaatttgtga tgctattgct ttatttgtaa     4920

ccatatgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttgctttttg     4980

cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag     5040

ggaacccact gcttaagcct caataaagct tgcctcgacc agcctcgact gtgccttcta     5100

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca     5160

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc     5220

attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata     5280

gcaggcatgc tggggatgcg gtgggctcta tggcctgcag ctgcattaat gaatcggcca     5340

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     5400

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     5460

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     5520

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     5580

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     5640

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     5700

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     5760

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     5820

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     5880

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     5940

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     6000

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     6060

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     6120

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     6180

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     6240

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     6300

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     6360

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     6420

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     6480

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     6540

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     6600

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     6660

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     6720

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     6780

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     6840

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     6900

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     6960

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     7020

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     7080

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     7140

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     7200

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttag          7255


<210>  11
<211>  7255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_scFvCD19-DmrB-BFP lentiviral vector

<400>  11
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccccccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcggag tgcaggtgga aaccatctcc ccaggagacg     3120

ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag     3180

atggaaagaa agttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca     3240

agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag     3300

ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc     3360

caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc ggccgctccg     3420

gtgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccg ggcccctcta     3480

gaagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca     3540

accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca     3600

tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta     3660

gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca     3720

agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg     3780

tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga     3840

tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg     3900

aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg     3960

ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca     4020

agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa     4080

gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat     4140

actgcgacct ccctagcaaa ctggggcaca agcttaattg agtcgaccga gcatcttacc     4200

gccatttata cccatatttg ttctgttttt cttgatttgg gtatacattt aaatgttaat     4260

agaacaaaat ggtggggcaa tcatttacat ttttagggat atgtaattac tagttcaggt     4320

gtattgccac aagacaaaca tgttaagaaa ctttcccgtt atttacgctc tgttcctgtt     4380

aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct     4440

ccttttacgc tgtgtggata tgctgcttta tagcctctgt atctagctat tgcttcccgt     4500

acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctctttt agaggagttg     4560

tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact     4620

ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg     4680

atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg     4740

ctgggcactg ataattccgt ggtgttgtca tcgaattcgg taccttttta aaagaaaagg     4800

ggggactgga agggctaatt cactcccaac gaagacaaga tatcataact tcgtatagca     4860

tacattatac gaagttataa tttatttgtg aaatttgtga tgctattgct ttatttgtaa     4920

ccatatgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttgctttttg     4980

cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag     5040

ggaacccact gcttaagcct caataaagct tgcctcgacc agcctcgact gtgccttcta     5100

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca     5160

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc     5220

attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata     5280

gcaggcatgc tggggatgcg gtgggctcta tggcctgcag ctgcattaat gaatcggcca     5340

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     5400

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     5460

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     5520

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     5580

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     5640

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     5700

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     5760

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     5820

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     5880

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     5940

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     6000

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     6060

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     6120

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     6180

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     6240

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     6300

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     6360

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     6420

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     6480

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     6540

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     6600

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     6660

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     6720

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     6780

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     6840

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     6900

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     6960

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     7020

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     7080

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     7140

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     7200

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttag          7255


<210>  12
<211>  7213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_scFvCD19-DmrC-BFP lentiviral vector

<400>  12
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccccccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcatcc tctggcatga gatgtggcat gaaggcctgg     3120

aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg     3180

agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc     3240

aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag     3300

ggaatgtcaa ggacctcctc caagcctggg acctctatta tcatgtgttc cgacgaatct     3360

caaagggcgg ccgctccggt gagggcagag gaagtcttct aacatgcggt gacgtggagg     3420

agaatccggg cccctctaga agcgagctga ttaaggagaa catgcacatg aagctgtaca     3480

tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct     3540

acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct     3600

tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg     3660

gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca     3720

catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc     3780

tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga     3840

agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg     3900

aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca     3960

agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg     4020

tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg     4080

aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattgag     4140

tcgaccgagc atcttaccgc catttatacc catatttgtt ctgtttttct tgatttgggt     4200

atacatttaa atgttaatag aacaaaatgg tggggcaatc atttacattt ttagggatat     4260

gtaattacta gttcaggtgt attgccacaa gacaaacatg ttaagaaact ttcccgttat     4320

ttacgctctg ttcctgttaa tcaacctctg gattacaaaa tttgtgaaag attgactgat     4380

attcttaact atgttgctcc ttttacgctg tgtggatatg ctgctttata gcctctgtat     4440

ctagctattg cttcccgtac ggctttcgtt ttctcctcct tgtataaatc ctggttgctg     4500

tctcttttag aggagttgtg gcccgttgtc cgtcaacgtg gcgtggtgtg ctctgtgttt     4560

gctgacgcaa cccccactgg ctggggcatt gccaccacct gtcaactcct ttctgggact     4620

ttcgctttcc ccctcccgat cgccacggca gaactcatcg ccgcctgcct tgcccgctgc     4680

tggacagggg ctaggttgct gggcactgat aattccgtgg tgttgtcatc gaattcggta     4740

cctttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata     4800

tcataacttc gtatagcata cattatacga agttataatt tatttgtgaa atttgtgatg     4860

ctattgcttt atttgtaacc atatgtttat ttgtgaaatt tgtgatgcta ttgctttatt     4920

tgtaaccatt gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg     4980

agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg cctcgaccag     5040

cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct     5100

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc     5160

attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg     5220

aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcctgcagct     5280

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc     5340

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca     5400

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg     5460

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     5520

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     5580

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     5640

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     5700

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     5760

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     5820

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     5880

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     5940

cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     6000

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     6060

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     6120

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     6180

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     6240

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc     6300

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     6360

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc     6420

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag     6480

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag     6540

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt     6600

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg     6660

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt     6720

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc     6780

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc     6840

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa     6900

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg     6960

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc     7020

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag     7080

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt     7140

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     7200

tgaatgtatt tag                                                        7213


<210>  13
<211>  2259
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  scFvCD19-TM-41BB-CD3z-BFP mRNA

<400>  13
auggcucugc cugugacagc ucugcugcug ccucuggccc ugcugcucca ugccgccaga       60

cccggauccg auauccagau gacccagacc accagcagcc ugagcgccag ccugggcgau      120

agagugacca ucagcugcag agccagccag gacaucagca aguaccugaa cugguaucag      180

cagaaacccg acggcaccgu gaagcugcug aucuaccaca ccagcagacu gcacagcggc      240

gugcccagca gauuuucugg cagcggcucc ggcaccgacu acagccugac caucuccaac      300

cuggaacagg aagauaucgc uaccuacuuc ugucagcaag gcaacacccu gcccuacacc      360

uucggcggag gcaccaagcu ggaaaucacc ggcagcacaa gcggcagcgg caagccugga      420

ucuggcgagg gaagcaccaa gggcgaagug aaacugcagg aaagcggccc uggacuggug      480

gccccaagcc agucucugag cgugaccugu accguguccg gcgugucccu gccugacuau      540

ggcguguccu ggaucagaca gccacccaga aagggccugg aauggcuggg agugaucugg      600

ggcagcgaga caaccuacua caacagcgcc cugaaguccc ggcugaccau caucaaggac      660

aacuccaaga gccagguguu ccugaagaug aacagccugc agaccgacga caccgccauc      720

uacuacugcg ccaagcacua cuacuacggc ggcagcuacg ccauggacua cuggggccag      780

ggcacaagcg ugaccguguc cagcgcuagc gccaagccua ccaccacccc ugccccuaga      840

ccuccaacac ccgccccaac aaucgccagc cagccucugu cucugaggcc cgaggcuugu      900

agaccagcug cuggcggagc cgugcacacc agaggacugg auuucgccug cgacaucuac      960

aucugggccc cucuggccgg cacauguggc gugcugcugc ugagccucgu gaucaccaug     1020

cauaaacggg gcagaaagaa acuccuguau auauucaaac aaccauuuau gagaccagua     1080

caaacuacuc aagaggaaga uggcuguagc ugccgauuuc cagaagaaga agaaggagga     1140

ugugaacugc gggugaaguu cagcagaagc gccgacgccc cugccuacca gcagggccag     1200

aaucagcugu acaacgagcu gaaccugggc agaagggaag aguacgacgu ccuggauaag     1260

cggagaggcc gggacccuga gaugggcggc aagccucggc ggaagaaccc ccaggaaggc     1320

cuguauaacg aacugcagaa agacaagaug gccgaggccu acagcgagau cggcaugaag     1380

ggcgagcgga ggcggggcaa gggccacgac ggccuguauc agggccuguc caccgccacc     1440

aaggauaccu acgacgcccu gcacaugcag gcccugcccc caaggggcgg ccgcuccggu     1500

gagggcagag gaagucuucu aacaugcggu gacguggagg agaauccggg ccccucuaga     1560

agcgagcuga uuaaggagaa caugcacaug aagcuguaca uggagggcac cguggacaac     1620

caucacuuca agugcacauc cgagggcgaa ggcaagcccu acgagggcac ccagaccaug     1680

agaaucaagg uggucgaggg cggcccucuc cccuucgccu ucgacauccu ggcuacuagc     1740

uuccucuacg gcagcaagac cuucaucaac cacacccagg gcauccccga cuucuucaag     1800

caguccuucc cugagggcuu cacaugggag agagucacca cauacgaaga cgggggcgug     1860

cugaccgcua cccaggacac cagccuccag gacggcugcc ucaucuacaa cgucaagauc     1920

agagggguga acuucacauc caacggcccu gugaugcaga agaaaacacu cggcugggag     1980

gccuucaccg agacgcugua ccccgcugac ggcggccugg aaggcagaaa cgacauggcc     2040

cugaagcucg ugggcgggag ccaucugauc gcaaacauca agaccacaua uagauccaag     2100

aaacccgcua agaaccucaa gaugccuggc gucuacuaug uggacuacag acuggaaaga     2160

aucaaggagg ccaacaacga gaccuacguc gagcagcacg agguggcagu ggccagauac     2220

ugcgaccucc cuagcaaacu ggggcacaag cuuaauuga                            2259


<210>  14
<211>  7597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_scFvCD19-TM-41BB-CD3z-BFP lentiviral vector

<400>  14
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccgatatcc     2340

agatgaccca gaccaccagc agcctgagcg ccagcctggg cgatagagtg accatcagct     2400

gcagagccag ccaggacatc agcaagtacc tgaactggta tcagcagaaa cccgacggca     2460

ccgtgaagct gctgatctac cacaccagca gactgcacag cggcgtgccc agcagatttt     2520

ctggcagcgg ctccggcacc gactacagcc tgaccatctc caacctggaa caggaagata     2580

tcgctaccta cttctgtcag caaggcaaca ccctgcccta caccttcggc ggaggcacca     2640

agctggaaat caccggcagc acaagcggca gcggcaagcc tggatctggc gagggaagca     2700

ccaagggcga agtgaaactg caggaaagcg gccctggact ggtggcccca agccagtctc     2760

tgagcgtgac ctgtaccgtg tccggcgtgt ccctgcctga ctatggcgtg tcctggatca     2820

gacagccacc cagaaagggc ctggaatggc tgggagtgat ctggggcagc gagacaacct     2880

actacaacag cgccctgaag tcccggctga ccatcatcaa ggacaactcc aagagccagg     2940

tgttcctgaa gatgaacagc ctgcagaccg acgacaccgc catctactac tgcgccaagc     3000

actactacta cggcggcagc tacgccatgg actactgggg ccagggcaca agcgtgaccg     3060

tgtccagcgc tagcgccaag cctaccacca cccctgcccc tagacctcca acacccgccc     3120

caacaatcgc cagccagcct ctgtctctga ggcccgaggc ttgtagacca gctgctggcg     3180

gagccgtgca caccagagga ctggatttcg cctgcgacat ctacatctgg gcccctctgg     3240

ccggcacatg tggcgtgctg ctgctgagcc tcgtgatcac catgcataaa cggggcagaa     3300

agaaactcct gtatatattc aaacaaccat ttatgagacc agtacaaact actcaagagg     3360

aagatggctg tagctgccga tttccagaag aagaagaagg aggatgtgaa ctgcgggtga     3420

agttcagcag aagcgccgac gcccctgcct accagcaggg ccagaatcag ctgtacaacg     3480

agctgaacct gggcagaagg gaagagtacg acgtcctgga taagcggaga ggccgggacc     3540

ctgagatggg cggcaagcct cggcggaaga acccccagga aggcctgtat aacgaactgc     3600

agaaagacaa gatggccgag gcctacagcg agatcggcat gaagggcgag cggaggcggg     3660

gcaagggcca cgacggcctg tatcagggcc tgtccaccgc caccaaggat acctacgacg     3720

ccctgcacat gcaggccctg cccccaaggg gcggccgctc cggtgagggc agaggaagtc     3780

ttctaacatg cggtgacgtg gaggagaatc cgggcccctc tagaagcgag ctgattaagg     3840

agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca     3900

catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg     3960

agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca     4020

agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg     4080

gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg     4140

acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca     4200

catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc     4260

tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg     4320

ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc     4380

tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca     4440

acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca     4500

aactggggca caagcttaat tgagtcgacc gagcatctta ccgccattta tacccatatt     4560

tgttctgttt ttcttgattt gggtatacat ttaaatgtta atagaacaaa atggtggggc     4620

aatcatttac atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa     4680

catgttaaga aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac     4740

aaaatttgtg aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga     4800

tatgctgctt tatagcctct gtatctagct attgcttccc gtacggcttt cgttttctcc     4860

tccttgtata aatcctggtt gctgtctctt ttagaggagt tgtggcccgt tgtccgtcaa     4920

cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc     4980

acctgtcaac tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc     5040

atcgccgcct gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc     5100

gtggtgttgt catcgaattc ggtacctttt taaaagaaaa ggggggactg gaagggctaa     5160

ttcactccca acgaagacaa gatatcataa cttcgtatag catacattat acgaagttat     5220

aatttatttg tgaaatttgt gatgctattg ctttatttgt aaccatatgt ttatttgtga     5280

aatttgtgat gctattgctt tatttgtaac cattgctttt tgcttgtact gggtctctct     5340

ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc     5400

ctcaataaag cttgcctcga ccagcctcga ctgtgccttc tagttgccag ccatctgttg     5460

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct     5520

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg     5580

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg     5640

cggtgggctc tatggcctgc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     5700

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     5760

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     5820

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     5880

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     5940

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     6000

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     6060

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc     6120

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     6180

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     6240

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     6300

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     6360

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     6420

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     6480

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     6540

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     6600

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     6660

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     6720

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     6780

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     6840

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     6900

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     6960

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     7020

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     7080

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     7140

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     7200

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     7260

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     7320

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     7380

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     7440

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     7500

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     7560

ttgtctcatg agcggataca tatttgaatg tatttag                              7597


<210>  15
<211>  324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DmrC-TM-41BB-CD3z protein

<400>  15

Met Gly Ser Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90                  95      


Ala Ser Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
            100                 105                 110         


Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
        115                 120                 125             


Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
    130                 135                 140                 


Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu 
145                 150                 155                 160 


Leu Leu Ser Leu Val Ile Thr Met His Lys Arg Gly Arg Lys Lys Leu 
                165                 170                 175     


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
            180                 185                 190         


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
        195                 200                 205             


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    210                 215                 220                 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
225                 230                 235                 240 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                245                 250                 255     


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
            260                 265                 270         


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
        275                 280                 285             


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
    290                 295                 300                 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
305                 310                 315                 320 


Pro Pro Arg Gly 
                


<210>  16
<211>  1032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SS-DmrC-TM-41BB-CD3z mRNA

<400>  16
auggcucugc cugugacagc ucugcugcug ccucuggccc ugcugcucca ugccgccaga       60

cccggaucca uccucuggca ugagaugugg caugaaggcc uggaagaggc aucucguuug      120

uacuuugggg aaaggaacgu gaaaggcaug uuugaggugc uggagcccuu gcaugcuaug      180

auggaacggg gcccccagac ucugaaggaa acauccuuua aucaggccua uggucgagau      240

uuaauggagg cccaagagug gugcaggaag uacaugaaau cagggaaugu caaggaccuc      300

cuccaagccu gggaccucua uuaucaugug uuccgacgaa ucucaaaggc uagcgccaag      360

ccuaccacca ccccugcccc uagaccucca acacccgccc caacaaucgc cagccagccu      420

cugucucuga ggcccgaggc uuguagacca gcugcuggcg gagccgugca caccagagga      480

cuggauuucg ccugcgacau cuacaucugg gccccucugg ccggcacaug uggcgugcug      540

cugcugagcc ucgugaucac caugcauaaa cggggcagaa agaaacuccu guauauauuc      600

aaacaaccau uuaugagacc aguacaaacu acucaagagg aagauggcug uagcugccga      660

uuuccagaag aagaagaagg aggaugugaa cugcggguga aguucagcag aagcgccgac      720

gccccugccu accagcaggg ccagaaucag cuguacaacg agcugaaccu gggcagaagg      780

gaagaguacg acguccugga uaagcggaga ggccgggacc cugagauggg cggcaagccu      840

cggcggaaga acccccagga aggccuguau aacgaacugc agaaagacaa gauggccgag      900

gccuacagcg agaucggcau gaagggcgag cggaggcggg gcaagggcca cgacggccug      960

uaucagggcc uguccaccgc caccaaggau accuacgacg cccugcacau gcaggcccug     1020

cccccaaggg gc                                                         1032


<210>  17
<211>  1032
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-DmrC-TM-41BB-CD3z DNA

<400>  17
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatcca tcctctggca tgagatgtgg catgaaggcc tggaagaggc atctcgtttg      120

tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt gcatgctatg      180

atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta tggtcgagat      240

ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt caaggacctc      300

ctccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaaggc tagcgccaag      360

cctaccacca cccctgcccc tagacctcca acacccgccc caacaatcgc cagccagcct      420

ctgtctctga ggcccgaggc ttgtagacca gctgctggcg gagccgtgca caccagagga      480

ctggatttcg cctgcgacat ctacatctgg gcccctctgg ccggcacatg tggcgtgctg      540

ctgctgagcc tcgtgatcac catgcataaa cggggcagaa agaaactcct gtatatattc      600

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga      660

tttccagaag aagaagaagg aggatgtgaa ctgcgggtga agttcagcag aagcgccgac      720

gcccctgcct accagcaggg ccagaatcag ctgtacaacg agctgaacct gggcagaagg      780

gaagagtacg acgtcctgga taagcggaga ggccgggacc ctgagatggg cggcaagcct      840

cggcggaaga acccccagga aggcctgtat aacgaactgc agaaagacaa gatggccgag      900

gcctacagcg agatcggcat gaagggcgag cggaggcggg gcaagggcca cgacggcctg      960

tatcagggcc tgtccaccgc caccaaggat acctacgacg ccctgcacat gcaggccctg     1020

cccccaaggg gc                                                         1032


<210>  18
<211>  1512
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-DmrC-spacer-TM-41BB-CD3z DNA

<400>  18
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatcca tcctctggca tgagatgtgg catgaaggcc tggaagaggc atctcgtttg      120

tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt gcatgctatg      180

atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta tggtcgagat      240

ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt caaggacctc      300

ctccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaaggc tagcgagagc      360

aagtacggac cgccctgccc accttgccct gcccccgagt tcctgggcgg acccagcgtg      420

ttcctgttcc cacccaagcc caaggacacc ctgatgatca gccggacccc cgaggtgacc      480

tgcgtggtgg tggacgtgag ccaggaagat cccgaggtcc agttcaattg gtacgtggac      540

ggcgtggaag tgcacaacgc caagaccaag cccagagagg aacagttcaa cagcacctac      600

cgggtggtgt ctgtgctgac cgtgctgcac caggactggc tgaacggcaa agaatacaag      660

tgcaaggtgt ccaacaaggg cctgcccagc agcatcgaaa agaccatcag caaggccaag      720

ggccagcctc gcgagcccca ggtgtacacc ctgcctccct cccaggaaga gatgaccaag      780

aaccaggtgt ccctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag      840

tgggagagca acggccagcc tgagaacaac tacaagacca cccctcccgt gctggacagc      900

gacggcagct tcttcctgta cagccggctg accgtggaca agagccggtg gcaggaaggc      960

aacgtcttta gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc     1020

ctgagcctgt ccctgggcaa gatgcataaa cggggcagaa agaaactcct gtatatattc     1080

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga     1140

tttccagaag aagaagaagg aggatgtgaa ctgcgggtga agttcagcag aagcgccgac     1200

gcccctgcct accagcaggg ccagaatcag ctgtacaacg agctgaacct gggcagaagg     1260

gaagagtacg acgtcctgga taagcggaga ggccgggacc ctgagatggg cggcaagcct     1320

cggcggaaga acccccagga aggcctgtat aacgaactgc agaaagacaa gatggccgag     1380

gcctacagcg agatcggcat gaagggcgag cggaggcggg gcaagggcca cgacggcctg     1440

tatcagggcc tgtccaccgc caccaaggat acctacgacg ccctgcacat gcaggccctg     1500

cccccaaggg gc                                                         1512


<210>  19
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DmrB-TM-41BB-CD3z protein

<400>  19

Met Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ala Ser 
            100                 105                 110         


Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
        115                 120                 125             


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
    130                 135                 140                 


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
145                 150                 155                 160 


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
                165                 170                 175     


Ser Leu Val Ile Thr Met His Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            180                 185                 190         


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        195                 200                 205             


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    210                 215                 220                 


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
225                 230                 235                 240 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
                245                 250                 255     


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
            260                 265                 270         


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
        275                 280                 285             


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
    290                 295                 300                 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
305                 310                 315                 320 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
                325                 330                 335     


Arg Gly 
        


<210>  20
<211>  1074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SS-DmrB-TM-41BB-CD3z mRNA

<400>  20
auggcucugc cugugacagc ucugcugcug ccucuggccc ugcugcucca ugccgccaga       60

cccggauccg gagugcaggu ggaaaccauc uccccaggag acgggcgcac cuuccccaag      120

cgcggccaga ccugcguggu gcacuacacc gggaugcuug aagauggaaa gaaaguugau      180

uccucccggg acagaaacaa gcccuuuaag uuuaugcuag gcaagcagga ggugauccga      240

ggcugggaag aagggguugc ccagaugagu gugggucaga gagccaaacu gacuauaucu      300

ccagauuaug ccuauggugc cacugggcac ccaggcauca ucccaccaca ugccacucuc      360

gucuucgaug uggagcuucu aaaacuggaa gcuagcgcca agccuaccac caccccugcc      420

ccuagaccuc caacacccgc cccaacaauc gccagccagc cucugucucu gaggcccgag      480

gcuuguagac cagcugcugg cggagccgug cacaccagag gacuggauuu cgccugcgac      540

aucuacaucu gggccccucu ggccggcaca uguggcgugc ugcugcugag ccucgugauc      600

accaugcaua aacggggcag aaagaaacuc cuguauauau ucaaacaacc auuuaugaga      660

ccaguacaaa cuacucaaga ggaagauggc uguagcugcc gauuuccaga agaagaagaa      720

ggaggaugug aacugcgggu gaaguucagc agaagcgccg acgccccugc cuaccagcag      780

ggccagaauc agcuguacaa cgagcugaac cugggcagaa gggaagagua cgacguccug      840

gauaagcgga gaggccggga cccugagaug ggcggcaagc cucggcggaa gaacccccag      900

gaaggccugu auaacgaacu gcagaaagac aagauggccg aggccuacag cgagaucggc      960

augaagggcg agcggaggcg gggcaagggc cacgacggcc uguaucaggg ccuguccacc     1020

gccaccaagg auaccuacga cgcccugcac augcaggccc ugcccccaag gggc           1074


<210>  21
<211>  1074
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-DmrB-TM-41BB-CD3z DNA

<400>  21
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatccg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag      120

cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaagttgat      180

tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga      240

ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct      300

ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc      360

gtcttcgatg tggagcttct aaaactggaa gctagcgcca agcctaccac cacccctgcc      420

cctagacctc caacacccgc cccaacaatc gccagccagc ctctgtctct gaggcccgag      480

gcttgtagac cagctgctgg cggagccgtg cacaccagag gactggattt cgcctgcgac      540

atctacatct gggcccctct ggccggcaca tgtggcgtgc tgctgctgag cctcgtgatc      600

accatgcata aacggggcag aaagaaactc ctgtatatat tcaaacaacc atttatgaga      660

ccagtacaaa ctactcaaga ggaagatggc tgtagctgcc gatttccaga agaagaagaa      720

ggaggatgtg aactgcgggt gaagttcagc agaagcgccg acgcccctgc ctaccagcag      780

ggccagaatc agctgtacaa cgagctgaac ctgggcagaa gggaagagta cgacgtcctg      840

gataagcgga gaggccggga ccctgagatg ggcggcaagc ctcggcggaa gaacccccag      900

gaaggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag cgagatcggc      960

atgaagggcg agcggaggcg gggcaagggc cacgacggcc tgtatcaggg cctgtccacc     1020

gccaccaagg atacctacga cgccctgcac atgcaggccc tgcccccaag gggc           1074


<210>  22
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-DmrB-spacer-TM-41BB-CD3z DNA

<400>  22
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatccg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag      120

cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaagttgat      180

tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga      240

ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct      300

ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc      360

gtcttcgatg tggagcttct aaaactggaa gctagcgaga gcaagtacgg accgccctgc      420

ccaccttgcc ctgcccccga gttcctgggc ggacccagcg tgttcctgtt cccacccaag      480

cccaaggaca ccctgatgat cagccggacc cccgaggtga cctgcgtggt ggtggacgtg      540

agccaggaag atcccgaggt ccagttcaat tggtacgtgg acggcgtgga agtgcacaac      600

gccaagacca agcccagaga ggaacagttc aacagcacct accgggtggt gtctgtgctg      660

accgtgctgc accaggactg gctgaacggc aaagaataca agtgcaaggt gtccaacaag      720

ggcctgccca gcagcatcga aaagaccatc agcaaggcca agggccagcc tcgcgagccc      780

caggtgtaca ccctgcctcc ctcccaggaa gagatgacca agaaccaggt gtccctgacc      840

tgcctggtga agggcttcta ccccagcgac atcgccgtgg agtgggagag caacggccag      900

cctgagaaca actacaagac cacccctccc gtgctggaca gcgacggcag cttcttcctg      960

tacagccggc tgaccgtgga caagagccgg tggcaggaag gcaacgtctt tagctgcagc     1020

gtgatgcacg aggccctgca caaccactac acccagaaga gcctgagcct gtccctgggc     1080

aagatgcata aacggggcag aaagaaactc ctgtatatat tcaaacaacc atttatgaga     1140

ccagtacaaa ctactcaaga ggaagatggc tgtagctgcc gatttccaga agaagaagaa     1200

ggaggatgtg aactgcgggt gaagttcagc agaagcgccg acgcccctgc ctaccagcag     1260

ggccagaatc agctgtacaa cgagctgaac ctgggcagaa gggaagagta cgacgtcctg     1320

gataagcgga gaggccggga ccctgagatg ggcggcaagc ctcggcggaa gaacccccag     1380

gaaggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag cgagatcggc     1440

atgaagggcg agcggaggcg gggcaagggc cacgacggcc tgtatcaggg cctgtccacc     1500

gccaccaagg atacctacga cgccctgcac atgcaggccc tgcccccaag gggc           1554


<210>  23
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DmrA-TM-41BB-CD3z protein

<400>  23

Met Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ala Ser 
            100                 105                 110         


Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
        115                 120                 125             


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
    130                 135                 140                 


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
145                 150                 155                 160 


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
                165                 170                 175     


Ser Leu Val Ile Thr Met His Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            180                 185                 190         


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        195                 200                 205             


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    210                 215                 220                 


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
225                 230                 235                 240 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
                245                 250                 255     


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
            260                 265                 270         


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
        275                 280                 285             


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
    290                 295                 300                 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
305                 310                 315                 320 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
                325                 330                 335     


Arg Gly 
        


<210>  24
<211>  1074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DmrA-TM-41BB-CD3z mRNA

<400>  24
auggcucugc cugugacagc ucugcugcug ccucuggccc ugcugcucca ugccgccaga       60

cccggauccg gagugcaggu ggaaaccauc uccccaggag acgggcgcac cuuccccaag      120

cgcggccaga ccugcguggu gcacuacacc gggaugcuug aagauggaaa gaaauuugau      180

uccucccggg acagaaacaa gcccuuuaag uuuaugcuag gcaagcagga ggugauccga      240

ggcugggaag aagggguugc ccagaugagu gugggucaga gagccaaacu gacuauaucu      300

ccagauuaug ccuauggugc cacugggcac ccaggcauca ucccaccaca ugccacucuc      360

gucuucgaug uggagcuucu aaaacuggaa gcuagcgcca agccuaccac caccccugcc      420

ccuagaccuc caacacccgc cccaacaauc gccagccagc cucugucucu gaggcccgag      480

gcuuguagac cagcugcugg cggagccgug cacaccagag gacuggauuu cgccugcgac      540

aucuacaucu gggccccucu ggccggcaca uguggcgugc ugcugcugag ccucgugauc      600

accaugcaua aacggggcag aaagaaacuc cuguauauau ucaaacaacc auuuaugaga      660

ccaguacaaa cuacucaaga ggaagauggc uguagcugcc gauuuccaga agaagaagaa      720

ggaggaugug aacugcgggu gaaguucagc agaagcgccg acgccccugc cuaccagcag      780

ggccagaauc agcuguacaa cgagcugaac cugggcagaa gggaagagua cgacguccug      840

gauaagcgga gaggccggga cccugagaug ggcggcaagc cucggcggaa gaacccccag      900

gaaggccugu auaacgaacu gcagaaagac aagauggccg aggccuacag cgagaucggc      960

augaagggcg agcggaggcg gggcaagggc cacgacggcc uguaucaggg ccuguccacc     1020

gccaccaagg auaccuacga cgcccugcac augcaggccc ugcccccaag gggc           1074


<210>  25
<211>  1074
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DmrA-TM-41BB-CD3z DNA

<400>  25
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatccg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag      120

cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat      180

tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga      240

ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct      300

ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc      360

gtcttcgatg tggagcttct aaaactggaa gctagcgcca agcctaccac cacccctgcc      420

cctagacctc caacacccgc cccaacaatc gccagccagc ctctgtctct gaggcccgag      480

gcttgtagac cagctgctgg cggagccgtg cacaccagag gactggattt cgcctgcgac      540

atctacatct gggcccctct ggccggcaca tgtggcgtgc tgctgctgag cctcgtgatc      600

accatgcata aacggggcag aaagaaactc ctgtatatat tcaaacaacc atttatgaga      660

ccagtacaaa ctactcaaga ggaagatggc tgtagctgcc gatttccaga agaagaagaa      720

ggaggatgtg aactgcgggt gaagttcagc agaagcgccg acgcccctgc ctaccagcag      780

ggccagaatc agctgtacaa cgagctgaac ctgggcagaa gggaagagta cgacgtcctg      840

gataagcgga gaggccggga ccctgagatg ggcggcaagc ctcggcggaa gaacccccag      900

gaaggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag cgagatcggc      960

atgaagggcg agcggaggcg gggcaagggc cacgacggcc tgtatcaggg cctgtccacc     1020

gccaccaagg atacctacga cgccctgcac atgcaggccc tgcccccaag gggc           1074


<210>  26
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DmrA-spacer-TM-41BB-CD3z DNA

<400>  26
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatccg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag      120

cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat      180

tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga      240

ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct      300

ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc      360

gtcttcgatg tggagcttct aaaactggaa gctagcgaga gcaagtacgg accgccctgc      420

ccaccttgcc ctgcccccga gttcctgggc ggacccagcg tgttcctgtt cccacccaag      480

cccaaggaca ccctgatgat cagccggacc cccgaggtga cctgcgtggt ggtggacgtg      540

agccaggaag atcccgaggt ccagttcaat tggtacgtgg acggcgtgga agtgcacaac      600

gccaagacca agcccagaga ggaacagttc aacagcacct accgggtggt gtctgtgctg      660

accgtgctgc accaggactg gctgaacggc aaagaataca agtgcaaggt gtccaacaag      720

ggcctgccca gcagcatcga aaagaccatc agcaaggcca agggccagcc tcgcgagccc      780

caggtgtaca ccctgcctcc ctcccaggaa gagatgacca agaaccaggt gtccctgacc      840

tgcctggtga agggcttcta ccccagcgac atcgccgtgg agtgggagag caacggccag      900

cctgagaaca actacaagac cacccctccc gtgctggaca gcgacggcag cttcttcctg      960

tacagccggc tgaccgtgga caagagccgg tggcaggaag gcaacgtctt tagctgcagc     1020

gtgatgcacg aggccctgca caaccactac acccagaaga gcctgagcct gtccctgggc     1080

aagatgcata aacggggcag aaagaaactc ctgtatatat tcaaacaacc atttatgaga     1140

ccagtacaaa ctactcaaga ggaagatggc tgtagctgcc gatttccaga agaagaagaa     1200

ggaggatgtg aactgcgggt gaagttcagc agaagcgccg acgcccctgc ctaccagcag     1260

ggccagaatc agctgtacaa cgagctgaac ctgggcagaa gggaagagta cgacgtcctg     1320

gataagcgga gaggccggga ccctgagatg ggcggcaagc ctcggcggaa gaacccccag     1380

gaaggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag cgagatcggc     1440

atgaagggcg agcggaggcg gggcaagggc cacgacggcc tgtatcaggg cctgtccacc     1500

gccaccaagg atacctacga cgccctgcac atgcaggccc tgcccccaag gggc           1554


<210>  27
<211>  7141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrC-TM-41BB-CD3z lentiviral vector

<400>  27
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccatcctct     2340

ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt ggggaaagga     2400

acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa cggggccccc     2460

agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg gaggcccaag     2520

agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa gcctgggacc     2580

tctattatca tgtgttccga cgaatctcaa aggctagcgc caagcctacc accacccctg     2640

cccctagacc tccaacaccc gccccaacaa tcgccagcca gcctctgtct ctgaggcccg     2700

aggcttgtag accagctgct ggcggagccg tgcacaccag aggactggat ttcgcctgcg     2760

acatctacat ctgggcccct ctggccggca catgtggcgt gctgctgctg agcctcgtga     2820

tcaccatgca taaacggggc agaaagaaac tcctgtatat attcaaacaa ccatttatga     2880

gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca gaagaagaag     2940

aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct gcctaccagc     3000

agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag tacgacgtcc     3060

tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg aagaaccccc     3120

aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac agcgagatcg     3180

gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag ggcctgtcca     3240

ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca aggggcggcc     3300

gctccggtga gggcagagga agtcttctaa catgcggtga cgtggaggag aatccgggcc     3360

cctctagaag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg     3420

tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc     3480

agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg     3540

ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact     3600

tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg     3660

ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg     3720

tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg     3780

gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg     3840

acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata     3900

gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac     3960

tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg     4020

ccagatactg cgacctccct agcaaactgg ggcacaagct taattgagtc gaccgagcat     4080

cttaccgcca tttataccca tatttgttct gtttttcttg atttgggtat acatttaaat     4140

gttaatagaa caaaatggtg gggcaatcat ttacattttt agggatatgt aattactagt     4200

tcaggtgtat tgccacaaga caaacatgtt aagaaacttt cccgttattt acgctctgtt     4260

cctgttaatc aacctctgga ttacaaaatt tgtgaaagat tgactgatat tcttaactat     4320

gttgctcctt ttacgctgtg tggatatgct gctttatagc ctctgtatct agctattgct     4380

tcccgtacgg ctttcgtttt ctcctccttg tataaatcct ggttgctgtc tcttttagag     4440

gagttgtggc ccgttgtccg tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc     4500

cccactggct ggggcattgc caccacctgt caactccttt ctgggacttt cgctttcccc     4560

ctcccgatcg ccacggcaga actcatcgcc gcctgccttg cccgctgctg gacaggggct     4620

aggttgctgg gcactgataa ttccgtggtg ttgtcatcga attcggtacc tttttaaaag     4680

aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc ataacttcgt     4740

atagcataca ttatacgaag ttataattta tttgtgaaat ttgtgatgct attgctttat     4800

ttgtaaccat atgtttattt gtgaaatttg tgatgctatt gctttatttg taaccattgc     4860

tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct     4920

aactagggaa cccactgctt aagcctcaat aaagcttgcc tcgaccagcc tcgactgtgc     4980

cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag     5040

gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta     5100

ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag     5160

acaatagcag gcatgctggg gatgcggtgg gctctatggc ctgcagctgc attaatgaat     5220

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     5280

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     5340

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     5400

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     5460

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     5520

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     5580

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     5640

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5700

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5760

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     5820

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     5880

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     5940

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     6000

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     6060

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     6120

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     6180

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     6240

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     6300

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     6360

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     6420

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     6480

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     6540

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     6600

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     6660

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     6720

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     6780

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     6840

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     6900

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     6960

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     7020

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     7080

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     7140

g                                                                     7141


<210>  28
<211>  7621
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrC-spacer-TM-41BB-CD3z lentiviral vector

<400>  28
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccatcctct     2340

ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt ggggaaagga     2400

acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa cggggccccc     2460

agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg gaggcccaag     2520

agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa gcctgggacc     2580

tctattatca tgtgttccga cgaatctcaa aggctagcga gagcaagtac ggaccgccct     2640

gcccaccttg ccctgccccc gagttcctgg gcggacccag cgtgttcctg ttcccaccca     2700

agcccaagga caccctgatg atcagccgga cccccgaggt gacctgcgtg gtggtggacg     2760

tgagccagga agatcccgag gtccagttca attggtacgt ggacggcgtg gaagtgcaca     2820

acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg gtgtctgtgc     2880

tgaccgtgct gcaccaggac tggctgaacg gcaaagaata caagtgcaag gtgtccaaca     2940

agggcctgcc cagcagcatc gaaaagacca tcagcaaggc caagggccag cctcgcgagc     3000

cccaggtgta caccctgcct ccctcccagg aagagatgac caagaaccag gtgtccctga     3060

cctgcctggt gaagggcttc taccccagcg acatcgccgt ggagtgggag agcaacggcc     3120

agcctgagaa caactacaag accacccctc ccgtgctgga cagcgacggc agcttcttcc     3180

tgtacagccg gctgaccgtg gacaagagcc ggtggcagga aggcaacgtc tttagctgca     3240

gcgtgatgca cgaggccctg cacaaccact acacccagaa gagcctgagc ctgtccctgg     3300

gcaagatgca taaacggggc agaaagaaac tcctgtatat attcaaacaa ccatttatga     3360

gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca gaagaagaag     3420

aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct gcctaccagc     3480

agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag tacgacgtcc     3540

tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg aagaaccccc     3600

aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac agcgagatcg     3660

gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag ggcctgtcca     3720

ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca aggggcggcc     3780

gctccggtga gggcagagga agtcttctaa catgcggtga cgtggaggag aatccgggcc     3840

cctctagaag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg     3900

tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc     3960

agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg     4020

ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact     4080

tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg     4140

ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg     4200

tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg     4260

gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg     4320

acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata     4380

gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac     4440

tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg     4500

ccagatactg cgacctccct agcaaactgg ggcacaagct taattgagtc gaccgagcat     4560

cttaccgcca tttataccca tatttgttct gtttttcttg atttgggtat acatttaaat     4620

gttaatagaa caaaatggtg gggcaatcat ttacattttt agggatatgt aattactagt     4680

tcaggtgtat tgccacaaga caaacatgtt aagaaacttt cccgttattt acgctctgtt     4740

cctgttaatc aacctctgga ttacaaaatt tgtgaaagat tgactgatat tcttaactat     4800

gttgctcctt ttacgctgtg tggatatgct gctttatagc ctctgtatct agctattgct     4860

tcccgtacgg ctttcgtttt ctcctccttg tataaatcct ggttgctgtc tcttttagag     4920

gagttgtggc ccgttgtccg tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc     4980

cccactggct ggggcattgc caccacctgt caactccttt ctgggacttt cgctttcccc     5040

ctcccgatcg ccacggcaga actcatcgcc gcctgccttg cccgctgctg gacaggggct     5100

aggttgctgg gcactgataa ttccgtggtg ttgtcatcga attcggtacc tttttaaaag     5160

aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc ataacttcgt     5220

atagcataca ttatacgaag ttataattta tttgtgaaat ttgtgatgct attgctttat     5280

ttgtaaccat atgtttattt gtgaaatttg tgatgctatt gctttatttg taaccattgc     5340

tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct     5400

aactagggaa cccactgctt aagcctcaat aaagcttgcc tcgaccagcc tcgactgtgc     5460

cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag     5520

gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta     5580

ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag     5640

acaatagcag gcatgctggg gatgcggtgg gctctatggc ctgcagctgc attaatgaat     5700

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     5760

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     5820

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     5880

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     5940

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     6000

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     6060

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     6120

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     6180

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     6240

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     6300

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     6360

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     6420

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     6480

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     6540

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     6600

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     6660

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     6720

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     6780

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     6840

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     6900

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     6960

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     7020

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     7080

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     7140

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     7200

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     7260

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     7320

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     7380

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     7440

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     7500

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     7560

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     7620

g                                                                     7621


<210>  29
<211>  7183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrB-TM-41BB-CD3z lentiviral vector

<400>  29
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccggagtgc     2340

aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg     2400

tggtgcacta caccgggatg cttgaagatg gaaagaaagt tgattcctcc cgggacagaa     2460

acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg     2520

ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg     2580

gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc     2640

ttctaaaact ggaagctagc gccaagccta ccaccacccc tgcccctaga cctccaacac     2700

ccgccccaac aatcgccagc cagcctctgt ctctgaggcc cgaggcttgt agaccagctg     2760

ctggcggagc cgtgcacacc agaggactgg atttcgcctg cgacatctac atctgggccc     2820

ctctggccgg cacatgtggc gtgctgctgc tgagcctcgt gatcaccatg cataaacggg     2880

gcagaaagaa actcctgtat atattcaaac aaccatttat gagaccagta caaactactc     2940

aagaggaaga tggctgtagc tgccgatttc cagaagaaga agaaggagga tgtgaactgc     3000

gggtgaagtt cagcagaagc gccgacgccc ctgcctacca gcagggccag aatcagctgt     3060

acaacgagct gaacctgggc agaagggaag agtacgacgt cctggataag cggagaggcc     3120

gggaccctga gatgggcggc aagcctcggc ggaagaaccc ccaggaaggc ctgtataacg     3180

aactgcagaa agacaagatg gccgaggcct acagcgagat cggcatgaag ggcgagcgga     3240

ggcggggcaa gggccacgac ggcctgtatc agggcctgtc caccgccacc aaggatacct     3300

acgacgccct gcacatgcag gccctgcccc caaggggcgg ccgctccggt gagggcagag     3360

gaagtcttct aacatgcggt gacgtggagg agaatccggg cccctctaga agcgagctga     3420

ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca     3480

agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg     3540

tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg     3600

gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc     3660

ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta     3720

cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga     3780

acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg     3840

agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg     3900

tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta     3960

agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg     4020

ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc     4080

ctagcaaact ggggcacaag cttaattgag tcgaccgagc atcttaccgc catttatacc     4140

catatttgtt ctgtttttct tgatttgggt atacatttaa atgttaatag aacaaaatgg     4200

tggggcaatc atttacattt ttagggatat gtaattacta gttcaggtgt attgccacaa     4260

gacaaacatg ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg     4320

gattacaaaa tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg     4380

tgtggatatg ctgctttata gcctctgtat ctagctattg cttcccgtac ggctttcgtt     4440

ttctcctcct tgtataaatc ctggttgctg tctcttttag aggagttgtg gcccgttgtc     4500

cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt     4560

gccaccacct gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca     4620

gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat     4680

aattccgtgg tgttgtcatc gaattcggta cctttttaaa agaaaagggg ggactggaag     4740

ggctaattca ctcccaacga agacaagata tcataacttc gtatagcata cattatacga     4800

agttataatt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atatgtttat     4860

ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt gctttttgct tgtactgggt     4920

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     4980

ttaagcctca ataaagcttg cctcgaccag cctcgactgt gccttctagt tgccagccat     5040

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     5100

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     5160

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     5220

gggatgcggt gggctctatg gcctgcagct gcattaatga atcggccaac gcgcggggag     5280

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt     5340

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     5400

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     5460

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     5520

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     5580

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     5640

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct     5700

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     5760

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     5820

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     5880

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat     5940

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     6000

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     6060

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     6120

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct     6180

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     6240

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     6300

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     6360

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     6420

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     6480

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     6540

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     6600

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     6660

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     6720

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     6780

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     6840

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     6900

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     6960

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     7020

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     7080

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca     7140

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tag                       7183


<210>  30
<211>  7663
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrB-spacer-TM-41BB-CD3z lentiviral vector

<400>  30
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccggagtgc     2340

aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg     2400

tggtgcacta caccgggatg cttgaagatg gaaagaaagt tgattcctcc cgggacagaa     2460

acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg     2520

ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg     2580

gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc     2640

ttctaaaact ggaagctagc gagagcaagt acggaccgcc ctgcccacct tgccctgccc     2700

ccgagttcct gggcggaccc agcgtgttcc tgttcccacc caagcccaag gacaccctga     2760

tgatcagccg gacccccgag gtgacctgcg tggtggtgga cgtgagccag gaagatcccg     2820

aggtccagtt caattggtac gtggacggcg tggaagtgca caacgccaag accaagccca     2880

gagaggaaca gttcaacagc acctaccggg tggtgtctgt gctgaccgtg ctgcaccagg     2940

actggctgaa cggcaaagaa tacaagtgca aggtgtccaa caagggcctg cccagcagca     3000

tcgaaaagac catcagcaag gccaagggcc agcctcgcga gccccaggtg tacaccctgc     3060

ctccctccca ggaagagatg accaagaacc aggtgtccct gacctgcctg gtgaagggct     3120

tctaccccag cgacatcgcc gtggagtggg agagcaacgg ccagcctgag aacaactaca     3180

agaccacccc tcccgtgctg gacagcgacg gcagcttctt cctgtacagc cggctgaccg     3240

tggacaagag ccggtggcag gaaggcaacg tctttagctg cagcgtgatg cacgaggccc     3300

tgcacaacca ctacacccag aagagcctga gcctgtccct gggcaagatg cataaacggg     3360

gcagaaagaa actcctgtat atattcaaac aaccatttat gagaccagta caaactactc     3420

aagaggaaga tggctgtagc tgccgatttc cagaagaaga agaaggagga tgtgaactgc     3480

gggtgaagtt cagcagaagc gccgacgccc ctgcctacca gcagggccag aatcagctgt     3540

acaacgagct gaacctgggc agaagggaag agtacgacgt cctggataag cggagaggcc     3600

gggaccctga gatgggcggc aagcctcggc ggaagaaccc ccaggaaggc ctgtataacg     3660

aactgcagaa agacaagatg gccgaggcct acagcgagat cggcatgaag ggcgagcgga     3720

ggcggggcaa gggccacgac ggcctgtatc agggcctgtc caccgccacc aaggatacct     3780

acgacgccct gcacatgcag gccctgcccc caaggggcgg ccgctccggt gagggcagag     3840

gaagtcttct aacatgcggt gacgtggagg agaatccggg cccctctaga agcgagctga     3900

ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca     3960

agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg     4020

tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg     4080

gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc     4140

ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta     4200

cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga     4260

acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg     4320

agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg     4380

tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta     4440

agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg     4500

ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc     4560

ctagcaaact ggggcacaag cttaattgag tcgaccgagc atcttaccgc catttatacc     4620

catatttgtt ctgtttttct tgatttgggt atacatttaa atgttaatag aacaaaatgg     4680

tggggcaatc atttacattt ttagggatat gtaattacta gttcaggtgt attgccacaa     4740

gacaaacatg ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg     4800

gattacaaaa tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg     4860

tgtggatatg ctgctttata gcctctgtat ctagctattg cttcccgtac ggctttcgtt     4920

ttctcctcct tgtataaatc ctggttgctg tctcttttag aggagttgtg gcccgttgtc     4980

cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt     5040

gccaccacct gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca     5100

gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat     5160

aattccgtgg tgttgtcatc gaattcggta cctttttaaa agaaaagggg ggactggaag     5220

ggctaattca ctcccaacga agacaagata tcataacttc gtatagcata cattatacga     5280

agttataatt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atatgtttat     5340

ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt gctttttgct tgtactgggt     5400

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     5460

ttaagcctca ataaagcttg cctcgaccag cctcgactgt gccttctagt tgccagccat     5520

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     5580

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     5640

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     5700

gggatgcggt gggctctatg gcctgcagct gcattaatga atcggccaac gcgcggggag     5760

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt     5820

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     5880

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     5940

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     6000

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     6060

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     6120

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct     6180

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     6240

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     6300

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     6360

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat     6420

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     6480

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     6540

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     6600

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct     6660

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     6720

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     6780

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     6840

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     6900

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     6960

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     7020

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     7080

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     7140

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     7200

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     7260

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     7320

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     7380

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     7440

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     7500

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     7560

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca     7620

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tag                       7663


<210>  31
<211>  7183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrA-TM-41BB-CD3z lentiviral vector

<400>  31
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccggagtgc     2340

aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg     2400

tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctcc cgggacagaa     2460

acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg     2520

ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg     2580

gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc     2640

ttctaaaact ggaagctagc gccaagccta ccaccacccc tgcccctaga cctccaacac     2700

ccgccccaac aatcgccagc cagcctctgt ctctgaggcc cgaggcttgt agaccagctg     2760

ctggcggagc cgtgcacacc agaggactgg atttcgcctg cgacatctac atctgggccc     2820

ctctggccgg cacatgtggc gtgctgctgc tgagcctcgt gatcaccatg cataaacggg     2880

gcagaaagaa actcctgtat atattcaaac aaccatttat gagaccagta caaactactc     2940

aagaggaaga tggctgtagc tgccgatttc cagaagaaga agaaggagga tgtgaactgc     3000

gggtgaagtt cagcagaagc gccgacgccc ctgcctacca gcagggccag aatcagctgt     3060

acaacgagct gaacctgggc agaagggaag agtacgacgt cctggataag cggagaggcc     3120

gggaccctga gatgggcggc aagcctcggc ggaagaaccc ccaggaaggc ctgtataacg     3180

aactgcagaa agacaagatg gccgaggcct acagcgagat cggcatgaag ggcgagcgga     3240

ggcggggcaa gggccacgac ggcctgtatc agggcctgtc caccgccacc aaggatacct     3300

acgacgccct gcacatgcag gccctgcccc caaggggcgg ccgctccggt gagggcagag     3360

gaagtcttct aacatgcggt gacgtggagg agaatccggg cccctctaga agcgagctga     3420

ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca     3480

agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg     3540

tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg     3600

gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc     3660

ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta     3720

cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga     3780

acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg     3840

agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg     3900

tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta     3960

agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg     4020

ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc     4080

ctagcaaact ggggcacaag cttaattgag tcgaccgagc atcttaccgc catttatacc     4140

catatttgtt ctgtttttct tgatttgggt atacatttaa atgttaatag aacaaaatgg     4200

tggggcaatc atttacattt ttagggatat gtaattacta gttcaggtgt attgccacaa     4260

gacaaacatg ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg     4320

gattacaaaa tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg     4380

tgtggatatg ctgctttata gcctctgtat ctagctattg cttcccgtac ggctttcgtt     4440

ttctcctcct tgtataaatc ctggttgctg tctcttttag aggagttgtg gcccgttgtc     4500

cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt     4560

gccaccacct gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca     4620

gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat     4680

aattccgtgg tgttgtcatc gaattcggta cctttttaaa agaaaagggg ggactggaag     4740

ggctaattca ctcccaacga agacaagata tcataacttc gtatagcata cattatacga     4800

agttataatt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atatgtttat     4860

ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt gctttttgct tgtactgggt     4920

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     4980

ttaagcctca ataaagcttg cctcgaccag cctcgactgt gccttctagt tgccagccat     5040

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     5100

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     5160

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     5220

gggatgcggt gggctctatg gcctgcagct gcattaatga atcggccaac gcgcggggag     5280

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt     5340

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     5400

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     5460

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     5520

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     5580

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     5640

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct     5700

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     5760

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     5820

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     5880

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat     5940

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     6000

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     6060

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     6120

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct     6180

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     6240

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     6300

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     6360

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     6420

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     6480

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     6540

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     6600

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     6660

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     6720

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     6780

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     6840

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     6900

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     6960

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     7020

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     7080

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca     7140

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tag                       7183


<210>  32
<211>  7663
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrA-spacer-TM-41BB-CD3z lentiviral vector

<400>  32
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccggagtgc     2340

aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg     2400

tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctcc cgggacagaa     2460

acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg     2520

ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg     2580

gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc     2640

ttctaaaact ggaagctagc gagagcaagt acggaccgcc ctgcccacct tgccctgccc     2700

ccgagttcct gggcggaccc agcgtgttcc tgttcccacc caagcccaag gacaccctga     2760

tgatcagccg gacccccgag gtgacctgcg tggtggtgga cgtgagccag gaagatcccg     2820

aggtccagtt caattggtac gtggacggcg tggaagtgca caacgccaag accaagccca     2880

gagaggaaca gttcaacagc acctaccggg tggtgtctgt gctgaccgtg ctgcaccagg     2940

actggctgaa cggcaaagaa tacaagtgca aggtgtccaa caagggcctg cccagcagca     3000

tcgaaaagac catcagcaag gccaagggcc agcctcgcga gccccaggtg tacaccctgc     3060

ctccctccca ggaagagatg accaagaacc aggtgtccct gacctgcctg gtgaagggct     3120

tctaccccag cgacatcgcc gtggagtggg agagcaacgg ccagcctgag aacaactaca     3180

agaccacccc tcccgtgctg gacagcgacg gcagcttctt cctgtacagc cggctgaccg     3240

tggacaagag ccggtggcag gaaggcaacg tctttagctg cagcgtgatg cacgaggccc     3300

tgcacaacca ctacacccag aagagcctga gcctgtccct gggcaagatg cataaacggg     3360

gcagaaagaa actcctgtat atattcaaac aaccatttat gagaccagta caaactactc     3420

aagaggaaga tggctgtagc tgccgatttc cagaagaaga agaaggagga tgtgaactgc     3480

gggtgaagtt cagcagaagc gccgacgccc ctgcctacca gcagggccag aatcagctgt     3540

acaacgagct gaacctgggc agaagggaag agtacgacgt cctggataag cggagaggcc     3600

gggaccctga gatgggcggc aagcctcggc ggaagaaccc ccaggaaggc ctgtataacg     3660

aactgcagaa agacaagatg gccgaggcct acagcgagat cggcatgaag ggcgagcgga     3720

ggcggggcaa gggccacgac ggcctgtatc agggcctgtc caccgccacc aaggatacct     3780

acgacgccct gcacatgcag gccctgcccc caaggggcgg ccgctccggt gagggcagag     3840

gaagtcttct aacatgcggt gacgtggagg agaatccggg cccctctaga agcgagctga     3900

ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca     3960

agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg     4020

tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg     4080

gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc     4140

ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta     4200

cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga     4260

acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg     4320

agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg     4380

tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta     4440

agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg     4500

ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc     4560

ctagcaaact ggggcacaag cttaattgag tcgaccgagc atcttaccgc catttatacc     4620

catatttgtt ctgtttttct tgatttgggt atacatttaa atgttaatag aacaaaatgg     4680

tggggcaatc atttacattt ttagggatat gtaattacta gttcaggtgt attgccacaa     4740

gacaaacatg ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg     4800

gattacaaaa tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg     4860

tgtggatatg ctgctttata gcctctgtat ctagctattg cttcccgtac ggctttcgtt     4920

ttctcctcct tgtataaatc ctggttgctg tctcttttag aggagttgtg gcccgttgtc     4980

cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt     5040

gccaccacct gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca     5100

gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat     5160

aattccgtgg tgttgtcatc gaattcggta cctttttaaa agaaaagggg ggactggaag     5220

ggctaattca ctcccaacga agacaagata tcataacttc gtatagcata cattatacga     5280

agttataatt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atatgtttat     5340

ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt gctttttgct tgtactgggt     5400

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     5460

ttaagcctca ataaagcttg cctcgaccag cctcgactgt gccttctagt tgccagccat     5520

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     5580

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     5640

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     5700

gggatgcggt gggctctatg gcctgcagct gcattaatga atcggccaac gcgcggggag     5760

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt     5820

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     5880

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     5940

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     6000

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     6060

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     6120

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct     6180

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     6240

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     6300

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     6360

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat     6420

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     6480

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     6540

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     6600

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct     6660

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     6720

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     6780

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     6840

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     6900

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     6960

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     7020

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     7080

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     7140

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     7200

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     7260

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     7320

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     7380

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     7440

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     7500

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     7560

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca     7620

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tag                       7663


<210>  33
<211>  7477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrB-DmrC-TM-41BB-CD3z lentiviral vector

<400>  33
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccggagtgc     2340

aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg     2400

tggtgcacta caccgggatg cttgaagatg gaaagaaagt tgattcctcc cgggacagaa     2460

acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg     2520

ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg     2580

gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc     2640

ttctaaaact ggaaggcgga ggtgggagca tcctctggca tgagatgtgg catgaaggcc     2700

tggaagaggc atctcgtttg tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc     2760

tggagccctt gcatgctatg atggaacggg gcccccagac tctgaaggaa acatccttta     2820

atcaggccta tggtcgagat ttaatggagg cccaagagtg gtgcaggaag tacatgaaat     2880

cagggaatgt caaggacctc ctccaagcct gggacctcta ttatcatgtg ttccgacgaa     2940

tctcaaaggc tagcgccaag cctaccacca cccctgcccc tagacctcca acacccgccc     3000

caacaatcgc cagccagcct ctgtctctga ggcccgaggc ttgtagacca gctgctggcg     3060

gagccgtgca caccagagga ctggatttcg cctgcgacat ctacatctgg gcccctctgg     3120

ccggcacatg tggcgtgctg ctgctgagcc tcgtgatcac catgcataaa cggggcagaa     3180

agaaactcct gtatatattc aaacaaccat ttatgagacc agtacaaact actcaagagg     3240

aagatggctg tagctgccga tttccagaag aagaagaagg aggatgtgaa ctgcgggtga     3300

agttcagcag aagcgccgac gcccctgcct accagcaggg ccagaatcag ctgtacaacg     3360

agctgaacct gggcagaagg gaagagtacg acgtcctgga taagcggaga ggccgggacc     3420

ctgagatggg cggcaagcct cggcggaaga acccccagga aggcctgtat aacgaactgc     3480

agaaagacaa gatggccgag gcctacagcg agatcggcat gaagggcgag cggaggcggg     3540

gcaagggcca cgacggcctg tatcagggcc tgtccaccgc caccaaggat acctacgacg     3600

ccctgcacat gcaggccctg cccccaaggg gcggccgctc cggtgagggc agaggaagtc     3660

ttctaacatg cggtgacgtg gaggagaatc cgggcccctc tagaagcgag ctgattaagg     3720

agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca     3780

catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg     3840

agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca     3900

agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg     3960

gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg     4020

acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca     4080

catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc     4140

tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg     4200

ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc     4260

tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca     4320

acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca     4380

aactggggca caagcttaat tgagtcgacc gagcatctta ccgccattta tacccatatt     4440

tgttctgttt ttcttgattt gggtatacat ttaaatgtta atagaacaaa atggtggggc     4500

aatcatttac atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa     4560

catgttaaga aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac     4620

aaaatttgtg aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga     4680

tatgctgctt tatagcctct gtatctagct attgcttccc gtacggcttt cgttttctcc     4740

tccttgtata aatcctggtt gctgtctctt ttagaggagt tgtggcccgt tgtccgtcaa     4800

cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc     4860

acctgtcaac tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc     4920

atcgccgcct gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc     4980

gtggtgttgt catcgaattc ggtacctttt taaaagaaaa ggggggactg gaagggctaa     5040

ttcactccca acgaagacaa gatatcataa cttcgtatag catacattat acgaagttat     5100

aatttatttg tgaaatttgt gatgctattg ctttatttgt aaccatatgt ttatttgtga     5160

aatttgtgat gctattgctt tatttgtaac cattgctttt tgcttgtact gggtctctct     5220

ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc     5280

ctcaataaag cttgcctcga ccagcctcga ctgtgccttc tagttgccag ccatctgttg     5340

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct     5400

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg     5460

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg     5520

cggtgggctc tatggcctgc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     5580

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     5640

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     5700

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     5760

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     5820

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     5880

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     5940

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc     6000

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     6060

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     6120

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     6180

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     6240

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     6300

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     6360

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     6420

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     6480

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     6540

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     6600

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     6660

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     6720

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     6780

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     6840

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     6900

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     6960

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     7020

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     7080

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     7140

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     7200

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     7260

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     7320

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     7380

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     7440

ttgtctcatg agcggataca tatttgaatg tatttag                              7477


<210>  34
<211>  7519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_DmrB-DmrA-TM-41BB-CD3z lentiviral vector

<400>  34
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccggagtgc     2340

aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg     2400

tggtgcacta caccgggatg cttgaagatg gaaagaaagt tgattcctcc cgggacagaa     2460

acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg     2520

ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg     2580

gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc     2640

ttctaaaact ggaaggcgga ggtgggagcg gagtgcaggt ggaaaccatc tccccaggag     2700

acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg     2760

aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag tttatgctag     2820

gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga     2880

gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca     2940

tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa gctagcgcca     3000

agcctaccac cacccctgcc cctagacctc caacacccgc cccaacaatc gccagccagc     3060

ctctgtctct gaggcccgag gcttgtagac cagctgctgg cggagccgtg cacaccagag     3120

gactggattt cgcctgcgac atctacatct gggcccctct ggccggcaca tgtggcgtgc     3180

tgctgctgag cctcgtgatc accatgcata aacggggcag aaagaaactc ctgtatatat     3240

tcaaacaacc atttatgaga ccagtacaaa ctactcaaga ggaagatggc tgtagctgcc     3300

gatttccaga agaagaagaa ggaggatgtg aactgcgggt gaagttcagc agaagcgccg     3360

acgcccctgc ctaccagcag ggccagaatc agctgtacaa cgagctgaac ctgggcagaa     3420

gggaagagta cgacgtcctg gataagcgga gaggccggga ccctgagatg ggcggcaagc     3480

ctcggcggaa gaacccccag gaaggcctgt ataacgaact gcagaaagac aagatggccg     3540

aggcctacag cgagatcggc atgaagggcg agcggaggcg gggcaagggc cacgacggcc     3600

tgtatcaggg cctgtccacc gccaccaagg atacctacga cgccctgcac atgcaggccc     3660

tgcccccaag gggcggccgc tccggtgagg gcagaggaag tcttctaaca tgcggtgacg     3720

tggaggagaa tccgggcccc tctagaagcg agctgattaa ggagaacatg cacatgaagc     3780

tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca     3840

agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct     3900

tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca     3960

cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag     4020

tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg     4080

gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga     4140

tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg     4200

gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa     4260

acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct     4320

actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc     4380

agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta     4440

attgagtcga ccgagcatct taccgccatt tatacccata tttgttctgt ttttcttgat     4500

ttgggtatac atttaaatgt taatagaaca aaatggtggg gcaatcattt acatttttag     4560

ggatatgtaa ttactagttc aggtgtattg ccacaagaca aacatgttaa gaaactttcc     4620

cgttatttac gctctgttcc tgttaatcaa cctctggatt acaaaatttg tgaaagattg     4680

actgatattc ttaactatgt tgctcctttt acgctgtgtg gatatgctgc tttatagcct     4740

ctgtatctag ctattgcttc ccgtacggct ttcgttttct cctccttgta taaatcctgg     4800

ttgctgtctc ttttagagga gttgtggccc gttgtccgtc aacgtggcgt ggtgtgctct     4860

gtgtttgctg acgcaacccc cactggctgg ggcattgcca ccacctgtca actcctttct     4920

gggactttcg ctttccccct cccgatcgcc acggcagaac tcatcgccgc ctgccttgcc     4980

cgctgctgga caggggctag gttgctgggc actgataatt ccgtggtgtt gtcatcgaat     5040

tcggtacctt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac     5100

aagatatcat aacttcgtat agcatacatt atacgaagtt ataatttatt tgtgaaattt     5160

gtgatgctat tgctttattt gtaaccatat gtttatttgt gaaatttgtg atgctattgc     5220

tttatttgta accattgctt tttgcttgta ctgggtctct ctggttagac cagatctgag     5280

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctc     5340

gaccagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc     5400

cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg     5460

catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca     5520

agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggcct     5580

gcagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     5640

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     5700

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     5760

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     5820

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     5880

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     5940

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     6000

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     6060

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     6120

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     6180

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     6240

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac     6300

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     6360

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     6420

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     6480

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     6540

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     6600

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     6660

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     6720

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     6780

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     6840

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     6900

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     6960

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     7020

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     7080

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     7140

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     7200

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     7260

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     7320

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     7380

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     7440

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     7500

catatttgaa tgtatttag                                                  7519


<210>  35
<211>  7672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a_CD19-Puro lentiviral vector

<400>  35
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggg ccgccaccat gccacctcct cgcctcctct tcttcctcct cttcctcacc     2280

cccatggaag tcaggcccga ggaacctcta gtggtgaagg tggaagaggg agataacgct     2340

gtgctgcagt gcctcaaggg gacctcagat ggccccactc agcagctgac ctggtctcgg     2400

gagtccccgc ttaaaccctt cttaaaactc agcctgggat tacctgggtt aggcatccac     2460

atgaggcccc tggccatctg gcttttcatc ttcaacgtct ctcaacagat gggaggcttc     2520

tacctgtgcc agccggggcc tccctctgag aaggcctggc agcctggctg gacagtcaat     2580

gtggagggca gcggggagct gttccggtgg aatgtttcgg acctaggtgg cctgggctgt     2640

ggcctgaaga acaggtcctc agagggcccc agctcccctt ccgggaagct catgagcccc     2700

aagctgtatg tgtgggccaa agaccgccct gagatctggg agggagagcc tccgtgtctc     2760

ccaccgaggg acagcctgaa ccagagcctc agccaggacc tcaccatggc ccctggctcc     2820

acactctggc tgtcctgtgg ggtaccccct gactctgtgt ccaggggccc cctctcctgg     2880

acccatgtgc accccaaggg gcctaagtca ttgctgagcc tagagctgaa ggacgatcgc     2940

ccggccagag atatgtgggt aatggagacg ggtctgttgt tgccccgggc cacagctcaa     3000

gacgctggaa agtattattg tcaccgtggc aacctgacca tgtcattcca cctggagatc     3060

actgctcggc cagtactatg gcactggctg ctgaggactg gtggctggaa ggtctcagct     3120

gtgactttgg cttatctgat cttctgcctg tgttcccttg tgggcattct tcatcttcaa     3180

agagccctgg tcctgaggag gaagaggaag cgtatgactg accccaccag gagatttttc     3240

aaagtgacgc ctcccccagg aagcgggccc cagaaccagt acgggaacgt gctgtctctc     3300

cccacaccca cctcaggcct cggacgcgcc cagcgttggg ccgcaggcct gggaggcact     3360

gccccgtctt atggaaaccc gagcagcgac gtccaggcgg atggagcctt ggggtcccgg     3420

agcccgccgg gagtgggccc agaagaagag gaaggggagg gctacgagga acctgacagt     3480

gaggaggact ccgagttcta tgagaacgac tccaaccttg ggcaggacca gctctcccag     3540

gatggcagcg gctacgagaa ccctgaggat gagcccctgg gtcctgagga cgaggactcc     3600

ttctccaacg ctgagagcta tgaaaatgaa gatgaggagc tcacacaacc cgtcgccagg     3660

acaatggact tcctgagccc tcatgggtca gcatgggatc cgagccggga agcaacctcc     3720

ctggcagggt cccagtccta tgaggatatg agaggaatcc tgtatgcagc ccctcagctc     3780

cgctccattc ggggccagcc tggacccaat catgaggaag atgcagactc ttatgagaac     3840

atggataatc ccgatgggcc agacccagcc tggggaggag ggggccgcat gggcacctgg     3900

agcactcgcg gcggccgctc cggtgagggc agaggaagtc ttctaacatg cggtgacgtg     3960

gaggagaatc cgggcccctc tagaaccgag tacaagccca cggtgcgcct cgccacccgc     4020

gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg     4080

cgccacaccg tcgatcccga ccgccacatc gagcgggtca ccgagctgca agaactcttc     4140

ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg     4200

gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg     4260

cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg     4320

gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac     4380

caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc     4440

gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg     4500

ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg     4560

acccgcaagc ccggtgccct cgagtgatga gagtcatcgt cgaccgagca tcttaccgcc     4620

atttataccc atatttgttc tgtttttctt gatttgggta tacatttaaa tgttaataga     4680

acaaaatggt ggggcaatca tttacatttt tagggatatg taattactag ttcaggtgta     4740

ttgccacaag acaaacatgt taagaaactt tcccgttatt tacgctctgt tcctgttaat     4800

caacctctgg attacaaaat ttgtgaaaga ttgactgata ttcttaacta tgttgctcct     4860

tttacgctgt gtggatatgc tgctttatag cctctgtatc tagctattgc ttcccgtacg     4920

gctttcgttt tctcctcctt gtataaatcc tggttgctgt ctcttttaga ggagttgtgg     4980

cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac ccccactggc     5040

tggggcattg ccaccacctg tcaactcctt tctgggactt tcgctttccc cctcccgatc     5100

gccacggcag aactcatcgc cgcctgcctt gcccgctgct ggacaggggc taggttgctg     5160

ggcactgata attccgtggt gttgtcatcg aattcggtac ctttttaaaa gaaaaggggg     5220

gactggaagg gctaattcac tcccaacgaa gacaagatat cataacttcg tatagcatac     5280

attatacgaa gttataattt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca     5340

tatgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccattg ctttttgctt     5400

gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga     5460

acccactgct taagcctcaa taaagcttgc ctcgaccagc ctcgactgtg ccttctagtt     5520

gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc     5580

ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt     5640

ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca     5700

ggcatgctgg ggatgcggtg ggctctatgg cctgcagctg cattaatgaa tcggccaacg     5760

cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct     5820

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     5880

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     5940

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     6000

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     6060

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     6120

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     6180

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     6240

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     6300

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     6360

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     6420

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     6480

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     6540

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     6600

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     6660

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     6720

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     6780

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     6840

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     6900

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     6960

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     7020

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg     7080

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     7140

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     7200

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     7260

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     7320

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     7380

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     7440

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     7500

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     7560

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     7620

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt ag             7672


<210>  36
<211>  6892
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a_CD20-Puro lentiviral vector

<400>  36
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggg ccgccaccat gacaacaccc agaaattcag taaatgggac tttcccggca     2280

gagccaatga aaggccctat tgctatgcaa tctggtccaa aaccactctt caggaggatg     2340

tcttcactgg tgggccccac gcaaagcttc ttcatgaggg aatctaagac tttgggggct     2400

gtccagatta tgaatgggct cttccacatt gccctgggag gtcttctgat gatcccagca     2460

gggatctatg cacccatctg tgtgactgtg tggtaccctc tctggggagg cattatgtat     2520

attatttccg gatcactcct ggcagcaacg gagaaaaact ccaggaagtg tttggtcaaa     2580

ggaaaaatga taatgaattc attgagcctc tttgctgcca tttctggaat gattctttca     2640

atcatggaca tacttaatat taaaatttcc cattttttaa aaatggagag tctgaatttt     2700

attagagctc acacaccata tattaacata tacaactgtg aaccagctaa tccctctgag     2760

aaaaactccc catctaccca atactgttac agcatacaat ctctgttctt gggcattttg     2820

tcagtgatgc tgatctttgc cttcttccag gaacttgtaa tagctggcat cgttgagaat     2880

gaatggaaaa gaacgtgctc cagacccaaa tctaacatag ttctcctgtc agcagaagaa     2940

aaaaaagaac agactattga aataaaagaa gaagtggttg ggctaactga aacatcttcc     3000

caaccaaaga atgaagaaga cattgaaatt attccaatcc aagaagagga agaagaagaa     3060

acagagacga actttccaga acctccccaa gatcaggaat cctcaccaat agaaaatgac     3120

agctctcctg gcggccgctc cggtgagggc agaggaagtc ttctaacatg cggtgacgtg     3180

gaggagaatc cgggcccctc tagaaccgag tacaagccca cggtgcgcct cgccacccgc     3240

gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg     3300

cgccacaccg tcgatcccga ccgccacatc gagcgggtca ccgagctgca agaactcttc     3360

ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg     3420

gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg     3480

cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg     3540

gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac     3600

caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc     3660

gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg     3720

ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg     3780

acccgcaagc ccggtgccct cgagtgatga gagtcatcgt cgaccgagca tcttaccgcc     3840

atttataccc atatttgttc tgtttttctt gatttgggta tacatttaaa tgttaataga     3900

acaaaatggt ggggcaatca tttacatttt tagggatatg taattactag ttcaggtgta     3960

ttgccacaag acaaacatgt taagaaactt tcccgttatt tacgctctgt tcctgttaat     4020

caacctctgg attacaaaat ttgtgaaaga ttgactgata ttcttaacta tgttgctcct     4080

tttacgctgt gtggatatgc tgctttatag cctctgtatc tagctattgc ttcccgtacg     4140

gctttcgttt tctcctcctt gtataaatcc tggttgctgt ctcttttaga ggagttgtgg     4200

cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac ccccactggc     4260

tggggcattg ccaccacctg tcaactcctt tctgggactt tcgctttccc cctcccgatc     4320

gccacggcag aactcatcgc cgcctgcctt gcccgctgct ggacaggggc taggttgctg     4380

ggcactgata attccgtggt gttgtcatcg aattcggtac ctttttaaaa gaaaaggggg     4440

gactggaagg gctaattcac tcccaacgaa gacaagatat cataacttcg tatagcatac     4500

attatacgaa gttataattt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca     4560

tatgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccattg ctttttgctt     4620

gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga     4680

acccactgct taagcctcaa taaagcttgc ctcgaccagc ctcgactgtg ccttctagtt     4740

gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc     4800

ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt     4860

ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca     4920

ggcatgctgg ggatgcggtg ggctctatgg cctgcagctg cattaatgaa tcggccaacg     4980

cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct     5040

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     5100

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     5160

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     5220

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     5280

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     5340

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     5400

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     5460

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     5520

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     5580

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     5640

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     5700

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     5760

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     5820

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     5880

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     5940

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     6000

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     6060

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     6120

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     6180

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     6240

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg     6300

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     6360

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     6420

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     6480

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     6540

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     6600

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     6660

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     6720

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     6780

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     6840

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt ag             6892


<210>  37
<211>  570
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-2xDmrB-DmrC-TM-41BB-CD3z protein

<400>  37

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser Gly Gly Val Gln Val Glu Thr Ile Ser 
            20                  25                  30          


Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val 
        35                  40                  45              


His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg 
    50                  55                  60                  


Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile 
65                  70                  75                  80  


Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala 
                85                  90                  95      


Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro 
            100                 105                 110         


Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Phe Leu 
        115                 120                 125             


Lys Leu Glu Ser Gly Thr Ser Gly Thr Ser Gly Val Gln Val Glu Thr 
    130                 135                 140                 


Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys 
145                 150                 155                 160 


Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser 
                165                 170                 175     


Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu 
            180                 185                 190         


Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln 
        195                 200                 205             


Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly 
    210                 215                 220                 


His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu 
225                 230                 235                 240 


Leu Leu Lys Leu Glu Gly Ser Arg Ser Ile Leu Trp His Glu Met Trp 
                245                 250                 255     


His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn 
            260                 265                 270         


Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu 
        275                 280                 285             


Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly 
    290                 295                 300                 


Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser 
305                 310                 315                 320 


Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val 
                325                 330                 335     


Phe Arg Arg Ile Ser Lys Ala Ser Ala Lys Pro Thr Thr Thr Pro Ala 
            340                 345                 350         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        355                 360                 365             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    370                 375                 380                 


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
385                 390                 395                 400 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Met His Lys 
                405                 410                 415     


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
            420                 425                 430         


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
        435                 440                 445             


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
    450                 455                 460                 


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
465                 470                 475                 480 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
                485                 490                 495     


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
            500                 505                 510         


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        515                 520                 525             


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    530                 535                 540                 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
545                 550                 555                 560 


Leu His Met Gln Ala Leu Pro Pro Arg Gly 
                565                 570 


<210>  38
<211>  1710
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SS-2xDmrB-DmrC-TM-41BB-CD3z mRNA

<400>  38
auggcucugc cugugacagc ucugcugcug ccucuggccc ugcugcucca ugccgccaga       60

cccggauccg gcggugucca agucgaaacu auaucgccug gcgauggcag aacguuuccc      120

aaacguggcc agaccugugu cguacacuau accggcaugc uagaggaugg gaaaaagguu      180

gauuccaguc gcgaucggaa caaaccguuu aaauucaugu uggggaagca agagguuauc      240

aggggauggg aagagggugu cgcgcaaaug ucgguugggc aacgugcgaa acucacaauu      300

uccccggauu acgcauacgg agcuaccgga cacccuggga uuaucccacc gcaugcgacg      360

cuaguguuug acguagaguu cuugaagcuc gaaucaggua caagcggcac uucuggcgua      420

cagguugaga caauuagucc cggagacgga cguacauucc caaagagagg gcaaacuugc      480

guaguccauu acacuggaau guuggaagac ggcaagaaag uggacaguuc aagagaccgc      540

aauaagccuu ucaaguuuau gcucggaaaa caggaaguca uacgcgguug ggaggaaggc      600

guggcucaga ugagcgucgg acagagggca aaguugacca ucagucccga cuaugcguau      660

ggcgcgacag gccaucccgg aaucauaccu ccccacgcaa ccuugguauu cgaugucgaa      720

cugcucaaau uagaggguag uagauccauc cucuggcaug agauguggca ugaaggccug      780

gaagaggcau cucguuugua cuuuggggaa aggaacguga aaggcauguu ugaggugcug      840

gagcccuugc augcuaugau ggaacggggc ccccagacuc ugaaggaaac auccuuuaau      900

caggccuaug gucgagauuu aauggaggcc caagaguggu gcaggaagua caugaaauca      960

gggaauguca aggaccuccu ccaagccugg gaccucuauu aucauguguu ccgacgaauc     1020

ucaaaggcua gcgccaagcc uaccaccacc ccugccccua gaccuccaac acccgcccca     1080

acaaucgcca gccagccucu gucucugagg cccgaggcuu guagaccagc ugcuggcgga     1140

gccguacaca ccagaggacu ggauuucgcc ugcgacaucu acaucugggc cccucuggcc     1200

ggcacaugug gcgugcugcu gcugagccuc gugaucacca ugcauaaacg gggcagaaag     1260

aaacuccugu auauauucaa acaaccauuu augagaccag uacaaacuac ucaagaggaa     1320

gauggcugua gcugccgauu uccagaagaa gaagaaggag gaugugaacu gcgggugaag     1380

uucagcagaa gcgccgacgc cccugccuac cagcagggcc agaaucagcu guacaacgag     1440

cugaaccugg gcagaaggga agaguacgac guccuggaua agcggagagg ccgggacccu     1500

gagaugggcg gcaagccucg gcggaagaac ccccaggaag gccuguauaa cgaacugcag     1560

aaagacaaga uggccgaggc cuacagcgag aucggcauga agggcgagcg gaggcggggc     1620

aagggccacg acggccugua ucagggccug uccaccgcca ccaaggauac cuacgacgcc     1680

cugcacaugc aggcccugcc cccaaggggc                                      1710


<210>  39
<211>  1710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-2xDmrB-DmrC-TM-41BB-CD3z DNA

<400>  39
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatccg gcggtgtcca agtcgaaact atatcgcctg gcgatggcag aacgtttccc      120

aaacgtggcc agacctgtgt cgtacactat accggcatgc tagaggatgg gaaaaaggtt      180

gattccagtc gcgatcggaa caaaccgttt aaattcatgt tggggaagca agaggttatc      240

aggggatggg aagagggtgt cgcgcaaatg tcggttgggc aacgtgcgaa actcacaatt      300

tccccggatt acgcatacgg agctaccgga caccctggga ttatcccacc gcatgcgacg      360

ctagtgtttg acgtagagtt cttgaagctc gaatcaggta caagcggcac ttctggcgta      420

caggttgaga caattagtcc cggagacgga cgtacattcc caaagagagg gcaaacttgc      480

gtagtccatt acactggaat gttggaagac ggcaagaaag tggacagttc aagagaccgc      540

aataagcctt tcaagtttat gctcggaaaa caggaagtca tacgcggttg ggaggaaggc      600

gtggctcaga tgagcgtcgg acagagggca aagttgacca tcagtcccga ctatgcgtat      660

ggcgcgacag gccatcccgg aatcatacct ccccacgcaa ccttggtatt cgatgtcgaa      720

ctgctcaaat tagagggtag tagatccatc ctctggcatg agatgtggca tgaaggcctg      780

gaagaggcat ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg      840

gagcccttgc atgctatgat ggaacggggc ccccagactc tgaaggaaac atcctttaat      900

caggcctatg gtcgagattt aatggaggcc caagagtggt gcaggaagta catgaaatca      960

gggaatgtca aggacctcct ccaagcctgg gacctctatt atcatgtgtt ccgacgaatc     1020

tcaaaggcta gcgccaagcc taccaccacc cctgccccta gacctccaac acccgcccca     1080

acaatcgcca gccagcctct gtctctgagg cccgaggctt gtagaccagc tgctggcgga     1140

gccgtacaca ccagaggact ggatttcgcc tgcgacatct acatctgggc ccctctggcc     1200

ggcacatgtg gcgtgctgct gctgagcctc gtgatcacca tgcataaacg gggcagaaag     1260

aaactcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa     1320

gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact gcgggtgaag     1380

ttcagcagaa gcgccgacgc ccctgcctac cagcagggcc agaatcagct gtacaacgag     1440

ctgaacctgg gcagaaggga agagtacgac gtcctggata agcggagagg ccgggaccct     1500

gagatgggcg gcaagcctcg gcggaagaac ccccaggaag gcctgtataa cgaactgcag     1560

aaagacaaga tggccgaggc ctacagcgag atcggcatga agggcgagcg gaggcggggc     1620

aagggccacg acggcctgta tcagggcctg tccaccgcca ccaaggatac ctacgacgcc     1680

ctgcacatgc aggccctgcc cccaaggggc                                      1710


<210>  40
<211>  7819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a_T7_2xDmrB-DmrC-TM-41BB-CD3z lentiviral vector

<400>  40
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccggcggtg     2340

tccaagtcga aactatatcg cctggcgatg gcagaacgtt tcccaaacgt ggccagacct     2400

gtgtcgtaca ctataccggc atgctagagg atgggaaaaa ggttgattcc agtcgcgatc     2460

ggaacaaacc gtttaaattc atgttgggga agcaagaggt tatcagggga tgggaagagg     2520

gtgtcgcgca aatgtcggtt gggcaacgtg cgaaactcac aatttccccg gattacgcat     2580

acggagctac cggacaccct gggattatcc caccgcatgc gacgctagtg tttgacgtag     2640

agttcttgaa gctcgaatca ggtacaagcg gcacttctgg cgtacaggtt gagacaatta     2700

gtcccggaga cggacgtaca ttcccaaaga gagggcaaac ttgcgtagtc cattacactg     2760

gaatgttgga agacggcaag aaagtggaca gttcaagaga ccgcaataag cctttcaagt     2820

ttatgctcgg aaaacaggaa gtcatacgcg gttgggagga aggcgtggct cagatgagcg     2880

tcggacagag ggcaaagttg accatcagtc ccgactatgc gtatggcgcg acaggccatc     2940

ccggaatcat acctccccac gcaaccttgg tattcgatgt cgaactgctc aaattagagg     3000

gtagtagatc catcctctgg catgagatgt ggcatgaagg cctggaagag gcatctcgtt     3060

tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt gctggagccc ttgcatgcta     3120

tgatggaacg gggcccccag actctgaagg aaacatcctt taatcaggcc tatggtcgag     3180

atttaatgga ggcccaagag tggtgcagga agtacatgaa atcagggaat gtcaaggacc     3240

tcctccaagc ctgggacctc tattatcatg tgttccgacg aatctcaaag gctagcgcca     3300

agcctaccac cacccctgcc cctagacctc caacacccgc cccaacaatc gccagccagc     3360

ctctgtctct gaggcccgag gcttgtagac cagctgctgg cggagccgta cacaccagag     3420

gactggattt cgcctgcgac atctacatct gggcccctct ggccggcaca tgtggcgtgc     3480

tgctgctgag cctcgtgatc accatgcata aacggggcag aaagaaactc ctgtatatat     3540

tcaaacaacc atttatgaga ccagtacaaa ctactcaaga ggaagatggc tgtagctgcc     3600

gatttccaga agaagaagaa ggaggatgtg aactgcgggt gaagttcagc agaagcgccg     3660

acgcccctgc ctaccagcag ggccagaatc agctgtacaa cgagctgaac ctgggcagaa     3720

gggaagagta cgacgtcctg gataagcgga gaggccggga ccctgagatg ggcggcaagc     3780

ctcggcggaa gaacccccag gaaggcctgt ataacgaact gcagaaagac aagatggccg     3840

aggcctacag cgagatcggc atgaagggcg agcggaggcg gggcaagggc cacgacggcc     3900

tgtatcaggg cctgtccacc gccaccaagg atacctacga cgccctgcac atgcaggccc     3960

tgcccccaag gggcggccgc tccggtgagg gcagaggaag tcttctaaca tgcggtgacg     4020

tggaggagaa tccgggcccc tctagaagcg agctgattaa ggagaacatg cacatgaagc     4080

tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca     4140

agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct     4200

tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca     4260

cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag     4320

tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg     4380

gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga     4440

tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg     4500

gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa     4560

acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct     4620

actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc     4680

agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta     4740

attgagtcga ccgagcatct taccgccatt tatacccata tttgttctgt ttttcttgat     4800

ttgggtatac atttaaatgt taatagaaca aaatggtggg gcaatcattt acatttttag     4860

ggatatgtaa ttactagttc aggtgtattg ccacaagaca aacatgttaa gaaactttcc     4920

cgttatttac gctctgttcc tgttaatcaa cctctggatt acaaaatttg tgaaagattg     4980

actgatattc ttaactatgt tgctcctttt acgctgtgtg gatatgctgc tttatagcct     5040

ctgtatctag ctattgcttc ccgtacggct ttcgttttct cctccttgta taaatcctgg     5100

ttgctgtctc ttttagagga gttgtggccc gttgtccgtc aacgtggcgt ggtgtgctct     5160

gtgtttgctg acgcaacccc cactggctgg ggcattgcca ccacctgtca actcctttct     5220

gggactttcg ctttccccct cccgatcgcc acggcagaac tcatcgccgc ctgccttgcc     5280

cgctgctgga caggggctag gttgctgggc actgataatt ccgtggtgtt gtcatcgaat     5340

tcggtacctt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac     5400

aagatatcat aacttcgtat agcatacatt atacgaagtt ataatttatt tgtgaaattt     5460

gtgatgctat tgctttattt gtaaccatat gtttatttgt gaaatttgtg atgctattgc     5520

tttatttgta accattgctt tttgcttgta ctgggtctct ctggttagac cagatctgag     5580

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctc     5640

gaccagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc     5700

cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg     5760

catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca     5820

agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggcct     5880

gcagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     5940

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     6000

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     6060

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     6120

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     6180

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     6240

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     6300

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     6360

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     6420

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     6480

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     6540

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac     6600

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     6660

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     6720

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     6780

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     6840

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     6900

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     6960

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     7020

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     7080

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     7140

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     7200

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     7260

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     7320

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     7380

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     7440

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     7500

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     7560

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     7620

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     7680

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     7740

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     7800

catatttgaa tgtatttag                                                  7819


<210>  41
<211>  741
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-DmrC-TM-41BB-CD3z protein

<400>  41

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Tyr Lys Asp Glu Gly Ser Asp Ile Gln Met Thr 
            20                  25                  30          


Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 
        35                  40                  45              


Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln 
    50                  55                  60                  


Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg 
65                  70                  75                  80  


Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                85                  90                  95      


Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr 
            100                 105                 110         


Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly 
        115                 120                 125             


Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly 
    130                 135                 140                 


Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly 
145                 150                 155                 160 


Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val 
                165                 170                 175     


Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro 
            180                 185                 190         


Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr 
        195                 200                 205             


Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp 
    210                 215                 220                 


Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 
225                 230                 235                 240 


Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser 
                245                 250                 255     


Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
            260                 265                 270         


Ala Ser Gly Ser Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp 
        275                 280                 285             


Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr 
    290                 295                 300                 


Gly Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn 
305                 310                 315                 320 


Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp 
                325                 330                 335     


Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr 
            340                 345                 350         


Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile 
        355                 360                 365             


Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
    370                 375                 380                 


Gly Gly Arg Ala Arg Tyr Lys Arg Ser Val Ser Gly Ser Gly Ala Thr 
385                 390                 395                 400 


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
                405                 410                 415     


Pro Ser Arg Ser Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu 
            420                 425                 430         


Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe 
        435                 440                 445             


Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr 
    450                 455                 460                 


Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu 
465                 470                 475                 480 


Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp 
                485                 490                 495     


Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser 
            500                 505                 510         


Lys Ala Ser Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
        515                 520                 525             


Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
    530                 535                 540                 


Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
545                 550                 555                 560 


Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
                565                 570                 575     


Leu Leu Leu Ser Leu Val Ile Thr Met His Lys Arg Gly Arg Lys Lys 
            580                 585                 590         


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
        595                 600                 605             


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
    610                 615                 620                 


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
625                 630                 635                 640 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                645                 650                 655     


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            660                 665                 670         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        675                 680                 685             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    690                 695                 700                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
705                 710                 715                 720 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                725                 730                 735     


Leu Pro Pro Arg Gly 
            740     


<210>  42
<211>  2223
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-DmrC-TM-41BB-CD3z DNA

<400>  42
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt       60

gactacaagg acgagggatc cgatatccag atgacccaga ccaccagcag cctgagcgcc      120

agcctgggcg atagagtgac catcagctgc agagccagcc aggacatcag caagtacctg      180

aactggtatc agcagaaacc cgacggcacc gtgaagctgc tgatctacca caccagcaga      240

ctgcacagcg gcgtgcccag cagattttct ggcagcggct ccggcaccga ctacagcctg      300

accatctcca acctggaaca ggaagatatc gctacctact tctgtcagca aggcaacacc      360

ctgccctaca ccttcggcgg aggcaccaag ctggaaatca ccggcagcac aagcggcagc      420

ggcaagcctg gatctggcga gggaagcacc aagggcgaag tgaaactgca ggaaagcggc      480

cctggactgg tggccccaag ccagtctctg agcgtgacct gtaccgtgtc cggcgtgtcc      540

ctgcctgact atggcgtgtc ctggatcaga cagccaccca gaaagggcct ggaatggctg      600

ggagtgatct ggggcagcga gacaacctac tacaacagcg ccctgaagtc ccggctgacc      660

atcatcaagg acaactccaa gagccaggtg ttcctgaaga tgaacagcct gcagaccgac      720

gacaccgcca tctactactg cgccaagcac tactactacg gcggcagcta cgccatggac      780

tactggggcc agggcacaag cgtgaccgtg tccagcgcta gcggctcagg aggagtgcag      840

gttgaaacca tctccccagg agacgggcgc accttcccga agcgcggaca gacatgcgtg      900

gtgcactaca ccgggatgct tgaagatgga aagaaattcg attcatcgcg ggacagaaac      960

aagcccttta agtttatgct gggcaagcag gaggtcatcc gaggctggga agaaggggtt     1020

gcccagatga gtgtcggcca gagagccaaa ctgactatat cacctgacta cgcctatggg     1080

gccactgggc accctggcat aattccgcca cacgccactc tcgtcttcga tgtggagctt     1140

ctaaaactgg aaggcggccg cgctcgttac aagcgaagtg tctcaggatc tggcgccacg     1200

aacttctctc tgttaaagca agcaggagat gttgaagaaa accccgggcc ttcaagatcc     1260

atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg     1320

gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg     1380

ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag     1440

gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cctccaagcc     1500

tgggacctct attatcatgt gttccgacga atctcaaagg ctagcgccaa gcctaccacc     1560

acccctgccc ctagacctcc aacacccgcc ccaacaatcg ccagccagcc tctgtctctg     1620

aggcccgagg cttgtagacc agctgctggc ggagccgtac acaccagagg actggatttc     1680

gcctgcgaca tctacatctg ggcccctctg gccggcacat gtggcgtgct gctgctgagc     1740

ctcgtgatca ccatgcataa acggggcaga aagaaactcc tgtatatatt caaacaacca     1800

tttatgagac cagtacaaac tactcaagag gaagatggct gtagctgccg atttccagaa     1860

gaagaagaag gaggatgtga actgcgggtg aagttcagca gaagcgccga cgcccctgcc     1920

taccagcagg gccagaatca gctgtacaac gagctgaacc tgggcagaag ggaagagtac     1980

gacgtcctgg ataagcggag aggccgggac cctgagatgg gcggcaagcc tcggcggaag     2040

aacccccagg aaggcctgta taacgaactg cagaaagaca agatggccga ggcctacagc     2100

gagatcggca tgaagggcga gcggaggcgg ggcaagggcc acgacggcct gtatcagggc     2160

ctgtccaccg ccaccaagga tacctacgac gccctgcaca tgcaggccct gcccccaagg     2220

ggc                                                                   2223


<210>  43
<211>  8306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-DmrC-TM-41BB-CD3z lentiviral vector

<400>  43
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggg ccgccaccat ggagacagac acactcctgc tatgggtact gctgctctgg     2280

gttccaggtt ccactggtga ctacaaggac gagggatccg atatccagat gacccagacc     2340

accagcagcc tgagcgccag cctgggcgat agagtgacca tcagctgcag agccagccag     2400

gacatcagca agtacctgaa ctggtatcag cagaaacccg acggcaccgt gaagctgctg     2460

atctaccaca ccagcagact gcacagcggc gtgcccagca gattttctgg cagcggctcc     2520

ggcaccgact acagcctgac catctccaac ctggaacagg aagatatcgc tacctacttc     2580

tgtcagcaag gcaacaccct gccctacacc ttcggcggag gcaccaagct ggaaatcacc     2640

ggcagcacaa gcggcagcgg caagcctgga tctggcgagg gaagcaccaa gggcgaagtg     2700

aaactgcagg aaagcggccc tggactggtg gccccaagcc agtctctgag cgtgacctgt     2760

accgtgtccg gcgtgtccct gcctgactat ggcgtgtcct ggatcagaca gccacccaga     2820

aagggcctgg aatggctggg agtgatctgg ggcagcgaga caacctacta caacagcgcc     2880

ctgaagtccc ggctgaccat catcaaggac aactccaaga gccaggtgtt cctgaagatg     2940

aacagcctgc agaccgacga caccgccatc tactactgcg ccaagcacta ctactacggc     3000

ggcagctacg ccatggacta ctggggccag ggcacaagcg tgaccgtgtc cagcgctagc     3060

ggctcaggag gagtgcaggt tgaaaccatc tccccaggag acgggcgcac cttcccgaag     3120

cgcggacaga catgcgtggt gcactacacc gggatgcttg aagatggaaa gaaattcgat     3180

tcatcgcggg acagaaacaa gccctttaag tttatgctgg gcaagcagga ggtcatccga     3240

ggctgggaag aaggggttgc ccagatgagt gtcggccaga gagccaaact gactatatca     3300

cctgactacg cctatggggc cactgggcac cctggcataa ttccgccaca cgccactctc     3360

gtcttcgatg tggagcttct aaaactggaa ggcggccgcg ctcgttacaa gcgaagtgtc     3420

tcaggatctg gcgccacgaa cttctctctg ttaaagcaag caggagatgt tgaagaaaac     3480

cccgggcctt caagatccat cctctggcat gagatgtggc atgaaggcct ggaagaggca     3540

tctcgtttgt actttgggga aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg     3600

catgctatga tggaacgggg cccccagact ctgaaggaaa catcctttaa tcaggcctat     3660

ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc     3720

aaggacctcc tccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaaaggct     3780

agcgccaagc ctaccaccac ccctgcccct agacctccaa cacccgcccc aacaatcgcc     3840

agccagcctc tgtctctgag gcccgaggct tgtagaccag ctgctggcgg agccgtacac     3900

accagaggac tggatttcgc ctgcgacatc tacatctggg cccctctggc cggcacatgt     3960

ggcgtgctgc tgctgagcct cgtgatcacc atgcataaac ggggcagaaa gaaactcctg     4020

tatatattca aacaaccatt tatgagacca gtacaaacta ctcaagagga agatggctgt     4080

agctgccgat ttccagaaga agaagaagga ggatgtgaac tgcgggtgaa gttcagcaga     4140

agcgccgacg cccctgccta ccagcagggc cagaatcagc tgtacaacga gctgaacctg     4200

ggcagaaggg aagagtacga cgtcctggat aagcggagag gccgggaccc tgagatgggc     4260

ggcaagcctc ggcggaagaa cccccaggaa ggcctgtata acgaactgca gaaagacaag     4320

atggccgagg cctacagcga gatcggcatg aagggcgagc ggaggcgggg caagggccac     4380

gacggcctgt atcagggcct gtccaccgcc accaaggata cctacgacgc cctgcacatg     4440

caggccctgc ccccaagggg cggccgctcc ggtgagggca gaggaagtct tctaacatgc     4500

ggtgacgtgg aggagaatcc gggcccctct agaagcgagc tgattaagga gaacatgcac     4560

atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc     4620

gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct     4680

ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc     4740

aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg     4800

gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc     4860

caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc     4920

cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct     4980

gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg     5040

atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct     5100

ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac     5160

gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac     5220

aagcttaatt gagtcgaccg agcatcttac cgccatttat acccatattt gttctgtttt     5280

tcttgatttg ggtatacatt taaatgttaa tagaacaaaa tggtggggca atcatttaca     5340

tttttaggga tatgtaatta ctagttcagg tgtattgcca caagacaaac atgttaagaa     5400

actttcccgt tatttacgct ctgttcctgt taatcaacct ctggattaca aaatttgtga     5460

aagattgact gatattctta actatgttgc tccttttacg ctgtgtggat atgctgcttt     5520

atagcctctg tatctagcta ttgcttcccg tacggctttc gttttctcct ccttgtataa     5580

atcctggttg ctgtctcttt tagaggagtt gtggcccgtt gtccgtcaac gtggcgtggt     5640

gtgctctgtg tttgctgacg caacccccac tggctggggc attgccacca cctgtcaact     5700

cctttctggg actttcgctt tccccctccc gatcgccacg gcagaactca tcgccgcctg     5760

ccttgcccgc tgctggacag gggctaggtt gctgggcact gataattccg tggtgttgtc     5820

atcgaattcg gtaccttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa     5880

cgaagacaag atatcataac ttcgtatagc atacattata cgaagttata atttatttgt     5940

gaaatttgtg atgctattgc tttatttgta accatatgtt tatttgtgaa atttgtgatg     6000

ctattgcttt atttgtaacc attgcttttt gcttgtactg ggtctctctg gttagaccag     6060

atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc     6120

ttgcctcgac cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     6180

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     6240

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     6300

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     6360

atggcctgca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg     6420

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag     6480

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag     6540

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc     6600

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc     6660

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc     6720

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt     6780

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg     6840

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat     6900

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     6960

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     7020

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     7080

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     7140

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     7200

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     7260

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     7320

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     7380

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     7440

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     7500

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     7560

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     7620

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     7680

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     7740

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     7800

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     7860

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     7920

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     7980

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     8040

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     8100

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     8160

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     8220

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     8280

gcggatacat atttgaatgt atttag                                          8306


<210>  44
<211>  741
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-FRB-TM-41BB-CD3z protein

<400>  44

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Tyr Lys Asp Glu Gly Ser Asp Ile Gln Met Thr 
            20                  25                  30          


Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 
        35                  40                  45              


Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln 
    50                  55                  60                  


Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg 
65                  70                  75                  80  


Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                85                  90                  95      


Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr 
            100                 105                 110         


Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly 
        115                 120                 125             


Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly 
    130                 135                 140                 


Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly 
145                 150                 155                 160 


Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val 
                165                 170                 175     


Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro 
            180                 185                 190         


Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr 
        195                 200                 205             


Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp 
    210                 215                 220                 


Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 
225                 230                 235                 240 


Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser 
                245                 250                 255     


Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
            260                 265                 270         


Ala Ser Gly Ser Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp 
        275                 280                 285             


Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr 
    290                 295                 300                 


Gly Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn 
305                 310                 315                 320 


Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp 
                325                 330                 335     


Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr 
            340                 345                 350         


Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile 
        355                 360                 365             


Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
    370                 375                 380                 


Gly Gly Arg Ala Arg Tyr Lys Arg Ser Val Ser Gly Ser Gly Ala Thr 
385                 390                 395                 400 


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
                405                 410                 415     


Pro Ser Arg Ser Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu 
            420                 425                 430         


Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe 
        435                 440                 445             


Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr 
    450                 455                 460                 


Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu 
465                 470                 475                 480 


Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp 
                485                 490                 495     


Leu Thr Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser 
            500                 505                 510         


Lys Ala Ser Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
        515                 520                 525             


Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
    530                 535                 540                 


Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
545                 550                 555                 560 


Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
                565                 570                 575     


Leu Leu Leu Ser Leu Val Ile Thr Met His Lys Arg Gly Arg Lys Lys 
            580                 585                 590         


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
        595                 600                 605             


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
    610                 615                 620                 


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
625                 630                 635                 640 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                645                 650                 655     


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            660                 665                 670         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        675                 680                 685             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    690                 695                 700                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
705                 710                 715                 720 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                725                 730                 735     


Leu Pro Pro Arg Gly 
            740     


<210>  45
<211>  2223
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-FRB-TM-41BB-CD3z DNA

<400>  45
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt       60

gactacaagg acgagggatc cgatatccag atgacccaga ccaccagcag cctgagcgcc      120

agcctgggcg atagagtgac catcagctgc agagccagcc aggacatcag caagtacctg      180

aactggtatc agcagaaacc cgacggcacc gtgaagctgc tgatctacca caccagcaga      240

ctgcacagcg gcgtgcccag cagattttct ggcagcggct ccggcaccga ctacagcctg      300

accatctcca acctggaaca ggaagatatc gctacctact tctgtcagca aggcaacacc      360

ctgccctaca ccttcggcgg aggcaccaag ctggaaatca ccggcagcac aagcggcagc      420

ggcaagcctg gatctggcga gggaagcacc aagggcgaag tgaaactgca ggaaagcggc      480

cctggactgg tggccccaag ccagtctctg agcgtgacct gtaccgtgtc cggcgtgtcc      540

ctgcctgact atggcgtgtc ctggatcaga cagccaccca gaaagggcct ggaatggctg      600

ggagtgatct ggggcagcga gacaacctac tacaacagcg ccctgaagtc ccggctgacc      660

atcatcaagg acaactccaa gagccaggtg ttcctgaaga tgaacagcct gcagaccgac      720

gacaccgcca tctactactg cgccaagcac tactactacg gcggcagcta cgccatggac      780

tactggggcc agggcacaag cgtgaccgtg tccagcgcta gcggctcagg aggagtgcag      840

gttgaaacca tctccccagg agacgggcgc accttcccga agcgcggaca gacatgcgtg      900

gtgcactaca ccgggatgct tgaagatgga aagaaattcg attcatcgcg ggacagaaac      960

aagcccttta agtttatgct gggcaagcag gaggtcatcc gaggctggga agaaggggtt     1020

gcccagatga gtgtcggcca gagagccaaa ctgactatat cacctgacta cgcctatggg     1080

gccactgggc accctggcat aattccgcca cacgccactc tcgtcttcga tgtggagctt     1140

ctaaaactgg aaggcggccg cgctcgttac aagcgaagtg tctcaggatc tggcgccacg     1200

aacttctctc tgttaaagca agcaggagat gttgaagaaa accccgggcc ttcaagatcc     1260

atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg     1320

gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg     1380

ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag     1440

gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cacccaagcc     1500

tgggacctct attatcatgt gttccgacga atctcaaagg ctagcgccaa gcctaccacc     1560

acccctgccc ctagacctcc aacacccgcc ccaacaatcg ccagccagcc tctgtctctg     1620

aggcccgagg cttgtagacc agctgctggc ggagccgtac acaccagagg actggatttc     1680

gcctgcgaca tctacatctg ggcccctctg gccggcacat gtggcgtgct gctgctgagc     1740

ctcgtgatca ccatgcataa acggggcaga aagaaactcc tgtatatatt caaacaacca     1800

tttatgagac cagtacaaac tactcaagag gaagatggct gtagctgccg atttccagaa     1860

gaagaagaag gaggatgtga actgcgggtg aagttcagca gaagcgccga cgcccctgcc     1920

taccagcagg gccagaatca gctgtacaac gagctgaacc tgggcagaag ggaagagtac     1980

gacgtcctgg ataagcggag aggccgggac cctgagatgg gcggcaagcc tcggcggaag     2040

aacccccagg aaggcctgta taacgaactg cagaaagaca agatggccga ggcctacagc     2100

gagatcggca tgaagggcga gcggaggcgg ggcaagggcc acgacggcct gtatcagggc     2160

ctgtccaccg ccaccaagga tacctacgac gccctgcaca tgcaggccct gcccccaagg     2220

ggc                                                                   2223


<210>  46
<211>  8306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-FRB-TM-41BB-CD3z lentiviral vector

<400>  46
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggg ccgccaccat ggagacagac acactcctgc tatgggtact gctgctctgg     2280

gttccaggtt ccactggtga ctacaaggac gagggatccg atatccagat gacccagacc     2340

accagcagcc tgagcgccag cctgggcgat agagtgacca tcagctgcag agccagccag     2400

gacatcagca agtacctgaa ctggtatcag cagaaacccg acggcaccgt gaagctgctg     2460

atctaccaca ccagcagact gcacagcggc gtgcccagca gattttctgg cagcggctcc     2520

ggcaccgact acagcctgac catctccaac ctggaacagg aagatatcgc tacctacttc     2580

tgtcagcaag gcaacaccct gccctacacc ttcggcggag gcaccaagct ggaaatcacc     2640

ggcagcacaa gcggcagcgg caagcctgga tctggcgagg gaagcaccaa gggcgaagtg     2700

aaactgcagg aaagcggccc tggactggtg gccccaagcc agtctctgag cgtgacctgt     2760

accgtgtccg gcgtgtccct gcctgactat ggcgtgtcct ggatcagaca gccacccaga     2820

aagggcctgg aatggctggg agtgatctgg ggcagcgaga caacctacta caacagcgcc     2880

ctgaagtccc ggctgaccat catcaaggac aactccaaga gccaggtgtt cctgaagatg     2940

aacagcctgc agaccgacga caccgccatc tactactgcg ccaagcacta ctactacggc     3000

ggcagctacg ccatggacta ctggggccag ggcacaagcg tgaccgtgtc cagcgctagc     3060

ggctcaggag gagtgcaggt tgaaaccatc tccccaggag acgggcgcac cttcccgaag     3120

cgcggacaga catgcgtggt gcactacacc gggatgcttg aagatggaaa gaaattcgat     3180

tcatcgcggg acagaaacaa gccctttaag tttatgctgg gcaagcagga ggtcatccga     3240

ggctgggaag aaggggttgc ccagatgagt gtcggccaga gagccaaact gactatatca     3300

cctgactacg cctatggggc cactgggcac cctggcataa ttccgccaca cgccactctc     3360

gtcttcgatg tggagcttct aaaactggaa ggcggccgcg ctcgttacaa gcgaagtgtc     3420

tcaggatctg gcgccacgaa cttctctctg ttaaagcaag caggagatgt tgaagaaaac     3480

cccgggcctt caagatccat cctctggcat gagatgtggc atgaaggcct ggaagaggca     3540

tctcgtttgt actttgggga aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg     3600

catgctatga tggaacgggg cccccagact ctgaaggaaa catcctttaa tcaggcctat     3660

ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc     3720

aaggacctca cccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaaaggct     3780

agcgccaagc ctaccaccac ccctgcccct agacctccaa cacccgcccc aacaatcgcc     3840

agccagcctc tgtctctgag gcccgaggct tgtagaccag ctgctggcgg agccgtacac     3900

accagaggac tggatttcgc ctgcgacatc tacatctggg cccctctggc cggcacatgt     3960

ggcgtgctgc tgctgagcct cgtgatcacc atgcataaac ggggcagaaa gaaactcctg     4020

tatatattca aacaaccatt tatgagacca gtacaaacta ctcaagagga agatggctgt     4080

agctgccgat ttccagaaga agaagaagga ggatgtgaac tgcgggtgaa gttcagcaga     4140

agcgccgacg cccctgccta ccagcagggc cagaatcagc tgtacaacga gctgaacctg     4200

ggcagaaggg aagagtacga cgtcctggat aagcggagag gccgggaccc tgagatgggc     4260

ggcaagcctc ggcggaagaa cccccaggaa ggcctgtata acgaactgca gaaagacaag     4320

atggccgagg cctacagcga gatcggcatg aagggcgagc ggaggcgggg caagggccac     4380

gacggcctgt atcagggcct gtccaccgcc accaaggata cctacgacgc cctgcacatg     4440

caggccctgc ccccaagggg cggccgctcc ggtgagggca gaggaagtct tctaacatgc     4500

ggtgacgtgg aggagaatcc gggcccctct agaagcgagc tgattaagga gaacatgcac     4560

atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc     4620

gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct     4680

ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc     4740

aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg     4800

gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc     4860

caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc     4920

cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct     4980

gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg     5040

atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct     5100

ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac     5160

gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac     5220

aagcttaatt gagtcgaccg agcatcttac cgccatttat acccatattt gttctgtttt     5280

tcttgatttg ggtatacatt taaatgttaa tagaacaaaa tggtggggca atcatttaca     5340

tttttaggga tatgtaatta ctagttcagg tgtattgcca caagacaaac atgttaagaa     5400

actttcccgt tatttacgct ctgttcctgt taatcaacct ctggattaca aaatttgtga     5460

aagattgact gatattctta actatgttgc tccttttacg ctgtgtggat atgctgcttt     5520

atagcctctg tatctagcta ttgcttcccg tacggctttc gttttctcct ccttgtataa     5580

atcctggttg ctgtctcttt tagaggagtt gtggcccgtt gtccgtcaac gtggcgtggt     5640

gtgctctgtg tttgctgacg caacccccac tggctggggc attgccacca cctgtcaact     5700

cctttctggg actttcgctt tccccctccc gatcgccacg gcagaactca tcgccgcctg     5760

ccttgcccgc tgctggacag gggctaggtt gctgggcact gataattccg tggtgttgtc     5820

atcgaattcg gtaccttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa     5880

cgaagacaag atatcataac ttcgtatagc atacattata cgaagttata atttatttgt     5940

gaaatttgtg atgctattgc tttatttgta accatatgtt tatttgtgaa atttgtgatg     6000

ctattgcttt atttgtaacc attgcttttt gcttgtactg ggtctctctg gttagaccag     6060

atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc     6120

ttgcctcgac cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     6180

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     6240

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     6300

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     6360

atggcctgca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg     6420

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag     6480

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag     6540

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc     6600

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc     6660

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc     6720

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt     6780

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg     6840

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat     6900

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     6960

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     7020

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     7080

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     7140

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     7200

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     7260

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     7320

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     7380

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     7440

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     7500

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     7560

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     7620

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     7680

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     7740

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     7800

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     7860

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     7920

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     7980

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     8040

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     8100

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     8160

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     8220

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     8280

gcggatacat atttgaatgt atttag                                          8306


<210>  47
<211>  967
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-2xDmrB-DmrC-TM-41BB-CD3z protein

<400>  47

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Tyr Lys Asp Glu Gly Ser Asp Ile Gln Met Thr 
            20                  25                  30          


Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 
        35                  40                  45              


Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln 
    50                  55                  60                  


Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg 
65                  70                  75                  80  


Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                85                  90                  95      


Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr 
            100                 105                 110         


Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly 
        115                 120                 125             


Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly 
    130                 135                 140                 


Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly 
145                 150                 155                 160 


Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val 
                165                 170                 175     


Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro 
            180                 185                 190         


Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr 
        195                 200                 205             


Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp 
    210                 215                 220                 


Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 
225                 230                 235                 240 


Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser 
                245                 250                 255     


Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
            260                 265                 270         


Ala Ser Gly Ser Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp 
        275                 280                 285             


Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr 
    290                 295                 300                 


Gly Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn 
305                 310                 315                 320 


Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp 
                325                 330                 335     


Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr 
            340                 345                 350         


Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile 
        355                 360                 365             


Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
    370                 375                 380                 


Gly Gly Arg Ala Arg Tyr Lys Arg Ser Val Ser Gly Ser Gly Ala Thr 
385                 390                 395                 400 


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
                405                 410                 415     


Pro Ser Arg Ser Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp 
            420                 425                 430         


Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr 
        435                 440                 445             


Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn 
    450                 455                 460                 


Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp 
465                 470                 475                 480 


Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr 
                485                 490                 495     


Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile 
            500                 505                 510         


Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Phe Leu Lys Leu Glu 
        515                 520                 525             


Ser Gly Thr Ser Gly Thr Ser Gly Val Gln Val Glu Thr Ile Ser Pro 
    530                 535                 540                 


Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His 
545                 550                 555                 560 


Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp 
                565                 570                 575     


Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg 
            580                 585                 590         


Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys 
        595                 600                 605             


Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly 
    610                 615                 620                 


Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys 
625                 630                 635                 640 


Leu Glu Gly Ser Arg Ser Ile Leu Trp His Glu Met Trp His Glu Gly 
                645                 650                 655     


Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly 
            660                 665                 670         


Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro 
        675                 680                 685             


Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu 
    690                 695                 700                 


Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val 
705                 710                 715                 720 


Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg 
                725                 730                 735     


Ile Ser Lys Ala Ser Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro 
            740                 745                 750         


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
        755                 760                 765             


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
    770                 775                 780                 


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
785                 790                 795                 800 


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Met His Lys Arg Gly Arg 
                805                 810                 815     


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
            820                 825                 830         


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
        835                 840                 845             


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    850                 855                 860                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
865                 870                 875                 880 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                885                 890                 895     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            900                 905                 910         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        915                 920                 925             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    930                 935                 940                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
945                 950                 955                 960 


Gln Ala Leu Pro Pro Arg Gly 
                965         


<210>  48
<211>  2901
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-2xDmrB-DmrC-TM-41BB-CD3z DNA

<400>  48
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt       60

gactacaagg acgagggatc cgatatccag atgacccaga ccaccagcag cctgagcgcc      120

agcctgggcg atagagtgac catcagctgc agagccagcc aggacatcag caagtacctg      180

aactggtatc agcagaaacc cgacggcacc gtgaagctgc tgatctacca caccagcaga      240

ctgcacagcg gcgtgcccag cagattttct ggcagcggct ccggcaccga ctacagcctg      300

accatctcca acctggaaca ggaagatatc gctacctact tctgtcagca aggcaacacc      360

ctgccctaca ccttcggcgg aggcaccaag ctggaaatca ccggcagcac aagcggcagc      420

ggcaagcctg gatctggcga gggaagcacc aagggcgaag tgaaactgca ggaaagcggc      480

cctggactgg tggccccaag ccagtctctg agcgtgacct gtaccgtgtc cggcgtgtcc      540

ctgcctgact atggcgtgtc ctggatcaga cagccaccca gaaagggcct ggaatggctg      600

ggagtgatct ggggcagcga gacaacctac tacaacagcg ccctgaagtc ccggctgacc      660

atcatcaagg acaactccaa gagccaggtg ttcctgaaga tgaacagcct gcagaccgac      720

gacaccgcca tctactactg cgccaagcac tactactacg gcggcagcta cgccatggac      780

tactggggcc agggcacaag cgtgaccgtg tccagcgcta gcggctcagg aggagtgcag      840

gttgaaacca tctccccagg agacgggcgc accttcccga agcgcggaca gacatgcgtg      900

gtgcactaca ccgggatgct tgaagatgga aagaaattcg attcatcgcg ggacagaaac      960

aagcccttta agtttatgct gggcaagcag gaggtcatcc gaggctggga agaaggggtt     1020

gcccagatga gtgtcggcca gagagccaaa ctgactatat cacctgacta cgcctatggg     1080

gccactgggc accctggcat aattccgcca cacgccactc tcgtcttcga tgtggagctt     1140

ctaaaactgg aaggcggccg cgctcgttac aagcgaagtg tctcaggatc tggcgccacg     1200

aacttctctc tgttaaagca agcaggagat gttgaagaaa accccgggcc ttcaagatcc     1260

ggcggtgtcc aagtcgaaac tatatcgcct ggcgatggca gaacgtttcc caaacgtggc     1320

cagacctgtg tcgtacacta taccggcatg ctagaggatg ggaaaaaggt tgattccagt     1380

cgcgatcgga acaaaccgtt taaattcatg ttggggaagc aagaggttat caggggatgg     1440

gaagagggtg tcgcgcaaat gtcggttggg caacgtgcga aactcacaat ttccccggat     1500

tacgcatacg gagctaccgg acaccctggg attatcccac cgcatgcgac gctagtgttt     1560

gacgtagagt tcttgaagct cgaatcaggt acaagcggca cttctggcgt acaggttgag     1620

acaattagtc ccggagacgg acgtacattc ccaaagagag ggcaaacttg cgtagtccat     1680

tacactggaa tgttggaaga cggcaagaaa gtggacagtt caagagaccg caataagcct     1740

ttcaagttta tgctcggaaa acaggaagtc atacgcggtt gggaggaagg cgtggctcag     1800

atgagcgtcg gacagagggc aaagttgacc atcagtcccg actatgcgta tggcgcgaca     1860

ggccatcccg gaatcatacc tccccacgca accttggtat tcgatgtcga actgctcaaa     1920

ttagagggta gtagatccat cctctggcat gagatgtggc atgaaggcct ggaagaggca     1980

tctcgtttgt actttgggga aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg     2040

catgctatga tggaacgggg cccccagact ctgaaggaaa catcctttaa tcaggcctat     2100

ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc     2160

aaggacctcc tccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaaaggct     2220

agcgccaagc ctaccaccac ccctgcccct agacctccaa cacccgcccc aacaatcgcc     2280

agccagcctc tgtctctgag gcccgaggct tgtagaccag ctgctggcgg agccgtacac     2340

accagaggac tggatttcgc ctgcgacatc tacatctggg cccctctggc cggcacatgt     2400

ggcgtgctgc tgctgagcct cgtgatcacc atgcataaac ggggcagaaa gaaactcctg     2460

tatatattca aacaaccatt tatgagacca gtacaaacta ctcaagagga agatggctgt     2520

agctgccgat ttccagaaga agaagaagga ggatgtgaac tgcgggtgaa gttcagcaga     2580

agcgccgacg cccctgccta ccagcagggc cagaatcagc tgtacaacga gctgaacctg     2640

ggcagaaggg aagagtacga cgtcctggat aagcggagag gccgggaccc tgagatgggc     2700

ggcaagcctc ggcggaagaa cccccaggaa ggcctgtata acgaactgca gaaagacaag     2760

atggccgagg cctacagcga gatcggcatg aagggcgagc ggaggcgggg caagggccac     2820

gacggcctgt atcagggcct gtccaccgcc accaaggata cctacgacgc cctgcacatg     2880

caggccctgc ccccaagggg c                                               2901


<210>  49
<211>  8984
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-scFvCD19-DmrA-fuP2A-2xDmrB-DmrC-TM-41BB-CD3z lentiviral vector

<400>  49
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggg ccgccaccat ggagacagac acactcctgc tatgggtact gctgctctgg     2280

gttccaggtt ccactggtga ctacaaggac gagggatccg atatccagat gacccagacc     2340

accagcagcc tgagcgccag cctgggcgat agagtgacca tcagctgcag agccagccag     2400

gacatcagca agtacctgaa ctggtatcag cagaaacccg acggcaccgt gaagctgctg     2460

atctaccaca ccagcagact gcacagcggc gtgcccagca gattttctgg cagcggctcc     2520

ggcaccgact acagcctgac catctccaac ctggaacagg aagatatcgc tacctacttc     2580

tgtcagcaag gcaacaccct gccctacacc ttcggcggag gcaccaagct ggaaatcacc     2640

ggcagcacaa gcggcagcgg caagcctgga tctggcgagg gaagcaccaa gggcgaagtg     2700

aaactgcagg aaagcggccc tggactggtg gccccaagcc agtctctgag cgtgacctgt     2760

accgtgtccg gcgtgtccct gcctgactat ggcgtgtcct ggatcagaca gccacccaga     2820

aagggcctgg aatggctggg agtgatctgg ggcagcgaga caacctacta caacagcgcc     2880

ctgaagtccc ggctgaccat catcaaggac aactccaaga gccaggtgtt cctgaagatg     2940

aacagcctgc agaccgacga caccgccatc tactactgcg ccaagcacta ctactacggc     3000

ggcagctacg ccatggacta ctggggccag ggcacaagcg tgaccgtgtc cagcgctagc     3060

ggctcaggag gagtgcaggt tgaaaccatc tccccaggag acgggcgcac cttcccgaag     3120

cgcggacaga catgcgtggt gcactacacc gggatgcttg aagatggaaa gaaattcgat     3180

tcatcgcggg acagaaacaa gccctttaag tttatgctgg gcaagcagga ggtcatccga     3240

ggctgggaag aaggggttgc ccagatgagt gtcggccaga gagccaaact gactatatca     3300

cctgactacg cctatggggc cactgggcac cctggcataa ttccgccaca cgccactctc     3360

gtcttcgatg tggagcttct aaaactggaa ggcggccgcg ctcgttacaa gcgaagtgtc     3420

tcaggatctg gcgccacgaa cttctctctg ttaaagcaag caggagatgt tgaagaaaac     3480

cccgggcctt caagatccgg cggtgtccaa gtcgaaacta tatcgcctgg cgatggcaga     3540

acgtttccca aacgtggcca gacctgtgtc gtacactata ccggcatgct agaggatggg     3600

aaaaaggttg attccagtcg cgatcggaac aaaccgttta aattcatgtt ggggaagcaa     3660

gaggttatca ggggatggga agagggtgtc gcgcaaatgt cggttgggca acgtgcgaaa     3720

ctcacaattt ccccggatta cgcatacgga gctaccggac accctgggat tatcccaccg     3780

catgcgacgc tagtgtttga cgtagagttc ttgaagctcg aatcaggtac aagcggcact     3840

tctggcgtac aggttgagac aattagtccc ggagacggac gtacattccc aaagagaggg     3900

caaacttgcg tagtccatta cactggaatg ttggaagacg gcaagaaagt ggacagttca     3960

agagaccgca ataagccttt caagtttatg ctcggaaaac aggaagtcat acgcggttgg     4020

gaggaaggcg tggctcagat gagcgtcgga cagagggcaa agttgaccat cagtcccgac     4080

tatgcgtatg gcgcgacagg ccatcccgga atcatacctc cccacgcaac cttggtattc     4140

gatgtcgaac tgctcaaatt agagggtagt agatccatcc tctggcatga gatgtggcat     4200

gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt     4260

gaggtgctgg agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca     4320

tcctttaatc aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac     4380

atgaaatcag ggaatgtcaa ggacctcctc caagcctggg acctctatta tcatgtgttc     4440

cgacgaatct caaaggctag cgccaagcct accaccaccc ctgcccctag acctccaaca     4500

cccgccccaa caatcgccag ccagcctctg tctctgaggc ccgaggcttg tagaccagct     4560

gctggcggag ccgtacacac cagaggactg gatttcgcct gcgacatcta catctgggcc     4620

cctctggccg gcacatgtgg cgtgctgctg ctgagcctcg tgatcaccat gcataaacgg     4680

ggcagaaaga aactcctgta tatattcaaa caaccattta tgagaccagt acaaactact     4740

caagaggaag atggctgtag ctgccgattt ccagaagaag aagaaggagg atgtgaactg     4800

cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca gaatcagctg     4860

tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa gcggagaggc     4920

cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg cctgtataac     4980

gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg     5040

aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac caaggatacc     5100

tacgacgccc tgcacatgca ggccctgccc ccaaggggcg gccgctccgg tgagggcaga     5160

ggaagtcttc taacatgcgg tgacgtggag gagaatccgg gcccctctag aagcgagctg     5220

attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc     5280

aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag     5340

gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac     5400

ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc     5460

cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct     5520

acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg     5580

aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc     5640

gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc     5700

gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct     5760

aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag     5820

gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc     5880

cctagcaaac tggggcacaa gcttaattga gtcgaccgag catcttaccg ccatttatac     5940

ccatatttgt tctgtttttc ttgatttggg tatacattta aatgttaata gaacaaaatg     6000

gtggggcaat catttacatt tttagggata tgtaattact agttcaggtg tattgccaca     6060

agacaaacat gttaagaaac tttcccgtta tttacgctct gttcctgtta atcaacctct     6120

ggattacaaa atttgtgaaa gattgactga tattcttaac tatgttgctc cttttacgct     6180

gtgtggatat gctgctttat agcctctgta tctagctatt gcttcccgta cggctttcgt     6240

tttctcctcc ttgtataaat cctggttgct gtctctttta gaggagttgt ggcccgttgt     6300

ccgtcaacgt ggcgtggtgt gctctgtgtt tgctgacgca acccccactg gctggggcat     6360

tgccaccacc tgtcaactcc tttctgggac tttcgctttc cccctcccga tcgccacggc     6420

agaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctaggttgc tgggcactga     6480

taattccgtg gtgttgtcat cgaattcggt acctttttaa aagaaaaggg gggactggaa     6540

gggctaattc actcccaacg aagacaagat atcataactt cgtatagcat acattatacg     6600

aagttataat ttatttgtga aatttgtgat gctattgctt tatttgtaac catatgttta     6660

tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tgctttttgc ttgtactggg     6720

tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg     6780

cttaagcctc aataaagctt gcctcgacca gcctcgactg tgccttctag ttgccagcca     6840

tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc     6900

ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg     6960

gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct     7020

ggggatgcgg tgggctctat ggcctgcagc tgcattaatg aatcggccaa cgcgcgggga     7080

gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg     7140

tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag     7200

aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc     7260

gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca     7320

aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt     7380

ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc     7440

tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc     7500

tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc     7560

ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact     7620

tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg     7680

ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta     7740

tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca     7800

aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa     7860

aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg     7920

aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc     7980

ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg     8040

acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat     8100

ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg     8160

gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa     8220

taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca     8280

tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc     8340

gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt     8400

cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa     8460

aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat     8520

cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct     8580

tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga     8640

gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag     8700

tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga     8760

gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca     8820

ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg     8880

cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc     8940

agggttattg tctcatgagc ggatacatat ttgaatgtat ttag                      8984


<210>  50
<211>  406
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-CD4TM protein

<400>  50

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr 
        275                 280                 285             


Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu 
    290                 295                 300                 


Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe 
305                 310                 315                 320 


Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly 
                325                 330                 335     


Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro 
            340                 345                 350         


Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His 
        355                 360                 365             


Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Arg 
    370                 375                 380                 


Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile 
385                 390                 395                 400 


Gly Leu Gly Ile Phe Phe 
                405     


<210>  51
<211>  1218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-CD4TM DNA

<400>  51
atgcccctgg gcctgctgtg gctgggcctg gccctgctgg gcgccctgca cgcccaggcc       60

ggatccgata tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgataga      120

gtgaccatca gctgcagagc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

cccagcagat tttctggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgctac ctacttctgt cagcaaggca acaccctgcc ctacaccttc      360

ggcggaggca ccaagctgga aatcaccggc agcacaagcg gcagcggcaa gcctggatct      420

ggcgagggaa gcaccaaggg cgaagtgaaa ctgcaggaaa gcggccctgg actggtggcc      480

ccaagccagt ctctgagcgt gacctgtacc gtgtccggcg tgtccctgcc tgactatggc      540

gtgtcctgga tcagacagcc acccagaaag ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

acaagcgtga ccgtgtccag cgctagcggc ggaggtggga gcggagtgca ggtggaaacc      840

atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac      900

accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt      960

aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg     1020

agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg     1080

cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg     1140

gaaggcggcc gcatggccct gattgtgctg gggggcgtcg ccggcctcct gcttttcatt     1200

gggctaggca tcttcttc                                                   1218


<210>  52
<211>  7336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a-T7-hScnSS-CD19scFv-DmrA-CD4TM lentiviral vector

<400>  52
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccacccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcggag tgcaggtgga aaccatctcc ccaggagacg     3120

ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag     3180

atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca     3240

agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag     3300

ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc     3360

caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc ggccgcatgg     3420

ccctgattgt gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct     3480

tcatgcattc cggtgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc     3540

cgggcccctc tagagtgagc aagggcgagg aggataacat ggccatcatc aaggagttca     3600

tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg     3660

agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg     3720

gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct     3780

acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca     3840

agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct     3900

ctctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg     3960

acggccccgt aatgcagaag aagactatgg gctgggaggc ctcctccgag cggatgtacc     4020

ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc     4080

actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg     4140

cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg     4200

aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt     4260

aagtcgaccg agcatcttac cgccatttat acccatattt gttctgtttt tcttgatttg     4320

ggtatacatt taaatgttaa tagaacaaaa tggtggggca atcatttaca tttttaggga     4380

tatgtaatta ctagttcagg tgtattgcca caagacaaac atgttaagaa actttcccgt     4440

tatttacgct ctgttcctgt taatcaacct ctggattaca aaatttgtga aagattgact     4500

gatattctta actatgttgc tccttttacg ctgtgtggat atgctgcttt atagcctctg     4560

tatctagcta ttgcttcccg tacggctttc gttttctcct ccttgtataa atcctggttg     4620

ctgtctcttt tagaggagtt gtggcccgtt gtccgtcaac gtggcgtggt gtgctctgtg     4680

tttgctgacg caacccccac tggctggggc attgccacca cctgtcaact cctttctggg     4740

actttcgctt tccccctccc gatcgccacg gcagaactca tcgccgcctg ccttgcccgc     4800

tgctggacag gggctaggtt gctgggcact gataattccg tggtgttgtc atcgaattcg     4860

gtaccttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag     4920

atatcataac ttcgtatagc atacattata cgaagttata atttatttgt gaaatttgtg     4980

atgctattgc tttatttgta accatatgtt tatttgtgaa atttgtgatg ctattgcttt     5040

atttgtaacc attgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct     5100

gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgcctcgac     5160

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     5220

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     5280

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg     5340

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcctgca     5400

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc     5460

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     5520

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     5580

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     5640

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     5700

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     5760

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     5820

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     5880

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     5940

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     6000

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     6060

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     6120

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     6180

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     6240

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     6300

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     6360

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     6420

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta     6480

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga     6540

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg     6600

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc     6660

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat     6720

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag     6780

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat     6840

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa     6900

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa     6960

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga     7020

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg     7080

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc     7140

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg     7200

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact     7260

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat     7320

atttgaatgt atttag                                                     7336


<210>  53
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-CD8hingeTM protein

<400>  53

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr 
        275                 280                 285             


Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu 
    290                 295                 300                 


Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe 
305                 310                 315                 320 


Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly 
                325                 330                 335     


Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro 
            340                 345                 350         


Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His 
        355                 360                 365             


Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Arg 
    370                 375                 380                 


Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
385                 390                 395                 400 


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
                405                 410                 415     


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
            420                 425                 430         


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
        435                 440                 445             


Ser Leu Val Ile Thr 
    450             


<210>  54
<211>  1359
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-CD8hingeTM DNA

<400>  54
atgcccctgg gcctgctgtg gctgggcctg gccctgctgg gcgccctgca cgcccaggcc       60

ggatccgata tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgataga      120

gtgaccatca gctgcagagc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

cccagcagat tttctggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgctac ctacttctgt cagcaaggca acaccctgcc ctacaccttc      360

ggcggaggca ccaagctgga aatcaccggc agcacaagcg gcagcggcaa gcctggatct      420

ggcgagggaa gcaccaaggg cgaagtgaaa ctgcaggaaa gcggccctgg actggtggcc      480

ccaagccagt ctctgagcgt gacctgtacc gtgtccggcg tgtccctgcc tgactatggc      540

gtgtcctgga tcagacagcc acccagaaag ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

acaagcgtga ccgtgtccag cgctagcggc ggaggtggga gcggagtgca ggtggaaacc      840

atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac      900

accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt      960

aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg     1020

agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg     1080

cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg     1140

gaaggcggcc gcgccaagcc taccaccacc cctgccccta gacctccaac acccgcccca     1200

acaatcgcca gccagcctct gtctctgagg cccgaggctt gtagaccagc tgctggcgga     1260

gccgtgcaca ccagaggact ggatttcgcc tgcgacatct acatctgggc ccctctggcc     1320

ggcacatgtg gcgtgctgct gctgagcctc gtgatcacc                            1359


<210>  55
<211>  7477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a-T7-hScnSS-CD19scFv-DmrA-CD8hingeTM lentiviral vector

<400>  55
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccacccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcggag tgcaggtgga aaccatctcc ccaggagacg     3120

ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag     3180

atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca     3240

agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag     3300

ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc     3360

caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc ggccgcgcca     3420

agcctaccac cacccctgcc cctagacctc caacacccgc cccaacaatc gccagccagc     3480

ctctgtctct gaggcccgag gcttgtagac cagctgctgg cggagccgtg cacaccagag     3540

gactggattt cgcctgcgac atctacatct gggcccctct ggccggcaca tgtggcgtgc     3600

tgctgctgag cctcgtgatc accatgcatt ccggtgaggg cagaggaagt cttctaacat     3660

gcggtgacgt ggaggagaat ccgggcccct ctagagtgag caagggcgag gaggataaca     3720

tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc     3780

acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca     3840

agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt     3900

tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc     3960

tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg     4020

tgaccgtgac ccaggactcc tctctgcagg acggcgagtt catctacaag gtgaagctgc     4080

gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg     4140

cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc     4200

tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga     4260

agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca     4320

acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg     4380

gcatggacga gctgtacaag taagtcgacc gagcatctta ccgccattta tacccatatt     4440

tgttctgttt ttcttgattt gggtatacat ttaaatgtta atagaacaaa atggtggggc     4500

aatcatttac atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa     4560

catgttaaga aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac     4620

aaaatttgtg aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga     4680

tatgctgctt tatagcctct gtatctagct attgcttccc gtacggcttt cgttttctcc     4740

tccttgtata aatcctggtt gctgtctctt ttagaggagt tgtggcccgt tgtccgtcaa     4800

cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc     4860

acctgtcaac tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc     4920

atcgccgcct gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc     4980

gtggtgttgt catcgaattc ggtacctttt taaaagaaaa ggggggactg gaagggctaa     5040

ttcactccca acgaagacaa gatatcataa cttcgtatag catacattat acgaagttat     5100

aatttatttg tgaaatttgt gatgctattg ctttatttgt aaccatatgt ttatttgtga     5160

aatttgtgat gctattgctt tatttgtaac cattgctttt tgcttgtact gggtctctct     5220

ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc     5280

ctcaataaag cttgcctcga ccagcctcga ctgtgccttc tagttgccag ccatctgttg     5340

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct     5400

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg     5460

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg     5520

cggtgggctc tatggcctgc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     5580

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     5640

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     5700

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     5760

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     5820

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     5880

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     5940

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc     6000

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     6060

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     6120

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     6180

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     6240

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     6300

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     6360

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     6420

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     6480

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     6540

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     6600

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     6660

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     6720

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     6780

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     6840

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     6900

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     6960

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     7020

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     7080

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     7140

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     7200

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     7260

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     7320

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     7380

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     7440

ttgtctcatg agcggataca tatttgaatg tatttag                              7477


<210>  56
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-Spacer-CD4TM protein

<400>  56

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr 
        275                 280                 285             


Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu 
    290                 295                 300                 


Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe 
305                 310                 315                 320 


Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly 
                325                 330                 335     


Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro 
            340                 345                 350         


Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His 
        355                 360                 365             


Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Arg 
    370                 375                 380                 


Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
385                 390                 395                 400 


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
                405                 410                 415     


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
            420                 425                 430         


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
        435                 440                 445             


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
    450                 455                 460                 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
465                 470                 475                 480 


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
                485                 490                 495     


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
            500                 505                 510         


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
        515                 520                 525             


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
    530                 535                 540                 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
545                 550                 555                 560 


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
                565                 570                 575     


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
            580                 585                 590         


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
        595                 600                 605             


Leu Ser Leu Gly Lys Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly 
    610                 615                 620                 


Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe 
625                 630                 635 


<210>  57
<211>  1905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-Spacer-CD4TM DNA

<400>  57
atgcccctgg gcctgctgtg gctgggcctg gccctgctgg gcgccctgca cgcccaggcc       60

ggatccgata tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgataga      120

gtgaccatca gctgcagagc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

cccagcagat tttctggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgctac ctacttctgt cagcaaggca acaccctgcc ctacaccttc      360

ggcggaggca ccaagctgga aatcaccggc agcacaagcg gcagcggcaa gcctggatct      420

ggcgagggaa gcaccaaggg cgaagtgaaa ctgcaggaaa gcggccctgg actggtggcc      480

ccaagccagt ctctgagcgt gacctgtacc gtgtccggcg tgtccctgcc tgactatggc      540

gtgtcctgga tcagacagcc acccagaaag ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

acaagcgtga ccgtgtccag cgctagcggc ggaggtggga gcggagtgca ggtggaaacc      840

atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac      900

accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt      960

aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg     1020

agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg     1080

cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg     1140

gaaggcggcc gcgagagcaa gtacggaccg ccctgcccac cttgccctgc ccccgagttc     1200

ctgggcggac ccagcgtgtt cctgttccca cccaagccca aggacaccct gatgatcagc     1260

cggacccccg aggtgacctg cgtggtggtg gacgtgagcc aggaagatcc cgaggtccag     1320

ttcaattggt acgtggacgg cgtggaagtg cacaacgcca agaccaagcc cagagaggaa     1380

cagttcaaca gcacctaccg ggtggtgtct gtgctgaccg tgctgcacca ggactggctg     1440

aacggcaaag aatacaagtg caaggtgtcc aacaagggcc tgcccagcag catcgaaaag     1500

accatcagca aggccaaggg ccagcctcgc gagccccagg tgtacaccct gcctccctcc     1560

caggaagaga tgaccaagaa ccaggtgtcc ctgacctgcc tggtgaaggg cttctacccc     1620

agcgacatcg ccgtggagtg ggagagcaac ggccagcctg agaacaacta caagaccacc     1680

cctcccgtgc tggacagcga cggcagcttc ttcctgtaca gccggctgac cgtggacaag     1740

agccggtggc aggaaggcaa cgtctttagc tgcagcgtga tgcacgaggc cctgcacaac     1800

cactacaccc agaagagcct gagcctgtcc ctgggcaaga tggccctgat tgtgctgggg     1860

ggcgtcgccg gcctcctgct tttcattggg ctaggcatct tcttc                     1905


<210>  58
<211>  8023
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a-T7-hScnSS-CD19scFv-DmrA-Spacer-CD4TM lentiviral vector

<400>  58
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccacccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcggag tgcaggtgga aaccatctcc ccaggagacg     3120

ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag     3180

atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca     3240

agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag     3300

ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc     3360

caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc ggccgcgaga     3420

gcaagtacgg accgccctgc ccaccttgcc ctgcccccga gttcctgggc ggacccagcg     3480

tgttcctgtt cccacccaag cccaaggaca ccctgatgat cagccggacc cccgaggtga     3540

cctgcgtggt ggtggacgtg agccaggaag atcccgaggt ccagttcaat tggtacgtgg     3600

acggcgtgga agtgcacaac gccaagacca agcccagaga ggaacagttc aacagcacct     3660

accgggtggt gtctgtgctg accgtgctgc accaggactg gctgaacggc aaagaataca     3720

agtgcaaggt gtccaacaag ggcctgccca gcagcatcga aaagaccatc agcaaggcca     3780

agggccagcc tcgcgagccc caggtgtaca ccctgcctcc ctcccaggaa gagatgacca     3840

agaaccaggt gtccctgacc tgcctggtga agggcttcta ccccagcgac atcgccgtgg     3900

agtgggagag caacggccag cctgagaaca actacaagac cacccctccc gtgctggaca     3960

gcgacggcag cttcttcctg tacagccggc tgaccgtgga caagagccgg tggcaggaag     4020

gcaacgtctt tagctgcagc gtgatgcacg aggccctgca caaccactac acccagaaga     4080

gcctgagcct gtccctgggc aagatggccc tgattgtgct ggggggcgtc gccggcctcc     4140

tgcttttcat tgggctaggc atcttcttca tgcattccgg tgagggcaga ggaagtcttc     4200

taacatgcgg tgacgtggag gagaatccgg gcccctctag agtgagcaag ggcgaggagg     4260

ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga     4320

acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga     4380

ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc     4440

ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact     4500

tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg     4560

gcgtggtgac cgtgacccag gactcctctc tgcaggacgg cgagttcatc tacaaggtga     4620

agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct     4680

gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc     4740

agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg     4800

ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct     4860

cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca     4920

ccggcggcat ggacgagctg tacaagtaag tcgaccgagc atcttaccgc catttatacc     4980

catatttgtt ctgtttttct tgatttgggt atacatttaa atgttaatag aacaaaatgg     5040

tggggcaatc atttacattt ttagggatat gtaattacta gttcaggtgt attgccacaa     5100

gacaaacatg ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg     5160

gattacaaaa tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg     5220

tgtggatatg ctgctttata gcctctgtat ctagctattg cttcccgtac ggctttcgtt     5280

ttctcctcct tgtataaatc ctggttgctg tctcttttag aggagttgtg gcccgttgtc     5340

cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt     5400

gccaccacct gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca     5460

gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat     5520

aattccgtgg tgttgtcatc gaattcggta cctttttaaa agaaaagggg ggactggaag     5580

ggctaattca ctcccaacga agacaagata tcataacttc gtatagcata cattatacga     5640

agttataatt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atatgtttat     5700

ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt gctttttgct tgtactgggt     5760

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     5820

ttaagcctca ataaagcttg cctcgaccag cctcgactgt gccttctagt tgccagccat     5880

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     5940

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     6000

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     6060

gggatgcggt gggctctatg gcctgcagct gcattaatga atcggccaac gcgcggggag     6120

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt     6180

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     6240

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     6300

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     6360

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     6420

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     6480

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct     6540

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     6600

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     6660

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     6720

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat     6780

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     6840

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     6900

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     6960

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct     7020

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     7080

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     7140

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     7200

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     7260

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     7320

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     7380

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     7440

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     7500

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     7560

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     7620

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     7680

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     7740

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     7800

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     7860

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     7920

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca     7980

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tag                       8023


<210>  59
<211>  417
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-CD52 GPI anchor protein

<400>  59

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr 
        275                 280                 285             


Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu 
    290                 295                 300                 


Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe 
305                 310                 315                 320 


Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly 
                325                 330                 335     


Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro 
            340                 345                 350         


Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His 
        355                 360                 365             


Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Arg 
    370                 375                 380                 


Thr Ser Gln Thr Ser Ser Pro Ser Ala Ser Ser Asn Ile Ser Gly Gly 
385                 390                 395                 400 


Ile Phe Leu Phe Phe Val Ala Asn Ala Ile Ile His Leu Phe Cys Phe 
                405                 410                 415     


Ser 
    


<210>  60
<211>  1251
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-CD19scFv-DmrA-CD52 GPI anchor DNA

<400>  60
atgcccctgg gcctgctgtg gctgggcctg gccctgctgg gcgccctgca cgcccaggcc       60

ggatccgata tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgataga      120

gtgaccatca gctgcagagc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

cccagcagat tttctggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgctac ctacttctgt cagcaaggca acaccctgcc ctacaccttc      360

ggcggaggca ccaagctgga aatcaccggc agcacaagcg gcagcggcaa gcctggatct      420

ggcgagggaa gcaccaaggg cgaagtgaaa ctgcaggaaa gcggccctgg actggtggcc      480

ccaagccagt ctctgagcgt gacctgtacc gtgtccggcg tgtccctgcc tgactatggc      540

gtgtcctgga tcagacagcc acccagaaag ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

acaagcgtga ccgtgtccag cgctagcggc ggaggtggga gcggagtgca ggtggaaacc      840

atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac      900

accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt      960

aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg     1020

agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg     1080

cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg     1140

gaaggcggcc gcaccagcca aaccagcagc ccctcagcat ccagcaacat aagcggaggc     1200

attttccttt tcttcgtggc caatgccata atccacctct tctgcttcag t              1251


<210>  61
<211>  7369
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a-T7-hScnSS-CD19scFv-DmrA-CD52 GPI anchor lentiviral 
       vector

<400>  61
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccacccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcggag tgcaggtgga aaccatctcc ccaggagacg     3120

ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag     3180

atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca     3240

agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag     3300

ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc     3360

caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc ggccgcacca     3420

gccaaaccag cagcccctca gcatccagca acataagcgg aggcattttc cttttcttcg     3480

tggccaatgc cataatccac ctcttctgct tcagtatgca ttccggtgag ggcagaggaa     3540

gtcttctaac atgcggtgac gtggaggaga atccgggccc ctctagagtg agcaagggcg     3600

aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct     3660

ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca     3720

cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc     3780

tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg     3840

actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg     3900

acggcggcgt ggtgaccgtg acccaggact cctctctgca ggacggcgag ttcatctaca     3960

aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacta     4020

tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga     4080

tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct     4140

acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca     4200

tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc     4260

actccaccgg cggcatggac gagctgtaca agtaagtcga ccgagcatct taccgccatt     4320

tatacccata tttgttctgt ttttcttgat ttgggtatac atttaaatgt taatagaaca     4380

aaatggtggg gcaatcattt acatttttag ggatatgtaa ttactagttc aggtgtattg     4440

ccacaagaca aacatgttaa gaaactttcc cgttatttac gctctgttcc tgttaatcaa     4500

cctctggatt acaaaatttg tgaaagattg actgatattc ttaactatgt tgctcctttt     4560

acgctgtgtg gatatgctgc tttatagcct ctgtatctag ctattgcttc ccgtacggct     4620

ttcgttttct cctccttgta taaatcctgg ttgctgtctc ttttagagga gttgtggccc     4680

gttgtccgtc aacgtggcgt ggtgtgctct gtgtttgctg acgcaacccc cactggctgg     4740

ggcattgcca ccacctgtca actcctttct gggactttcg ctttccccct cccgatcgcc     4800

acggcagaac tcatcgccgc ctgccttgcc cgctgctgga caggggctag gttgctgggc     4860

actgataatt ccgtggtgtt gtcatcgaat tcggtacctt tttaaaagaa aaggggggac     4920

tggaagggct aattcactcc caacgaagac aagatatcat aacttcgtat agcatacatt     4980

atacgaagtt ataatttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatat     5040

gtttatttgt gaaatttgtg atgctattgc tttatttgta accattgctt tttgcttgta     5100

ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc     5160

cactgcttaa gcctcaataa agcttgcctc gaccagcctc gactgtgcct tctagttgcc     5220

agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca     5280

ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta     5340

ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc     5400

atgctgggga tgcggtgggc tctatggcct gcagctgcat taatgaatcg gccaacgcgc     5460

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg     5520

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     5580

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     5640

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     5700

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     5760

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     5820

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     5880

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     5940

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     6000

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     6060

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt     6120

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     6180

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     6240

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     6300

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     6360

gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg     6420

gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg     6480

ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc     6540

atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc     6600

agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc     6660

ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag     6720

tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat     6780

ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg     6840

caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt     6900

gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag     6960

atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg     7020

accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt     7080

aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct     7140

gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac     7200

tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat     7260

aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat     7320

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttag                 7369


<210>  62
<211>  7141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_FRB-TM-41BB-CD3z lentiviral vector

<400>  62
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatggct ctgcctgtga     2280

cagctctgct gctgcctctg gccctgctgc tccatgccgc cagacccgga tccatcctct     2340

ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt ggggaaagga     2400

acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa cggggccccc     2460

agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg gaggcccaag     2520

agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcacccaa gcctgggacc     2580

tctattatca tgtgttccga cgaatctcaa aggctagcgc caagcctacc accacccctg     2640

cccctagacc tccaacaccc gccccaacaa tcgccagcca gcctctgtct ctgaggcccg     2700

aggcttgtag accagctgct ggcggagccg tgcacaccag aggactggat ttcgcctgcg     2760

acatctacat ctgggcccct ctggccggca catgtggcgt gctgctgctg agcctcgtga     2820

tcaccatgca taaacggggc agaaagaaac tcctgtatat attcaaacaa ccatttatga     2880

gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca gaagaagaag     2940

aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct gcctaccagc     3000

agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag tacgacgtcc     3060

tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg aagaaccccc     3120

aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac agcgagatcg     3180

gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag ggcctgtcca     3240

ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca aggggcggcc     3300

gctccggtga gggcagagga agtcttctaa catgcggtga cgtggaggag aatccgggcc     3360

cctctagaag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg     3420

tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc     3480

agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg     3540

ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact     3600

tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg     3660

ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg     3720

tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg     3780

gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg     3840

acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata     3900

gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac     3960

tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg     4020

ccagatactg cgacctccct agcaaactgg ggcacaagct taattgagtc gaccgagcat     4080

cttaccgcca tttataccca tatttgttct gtttttcttg atttgggtat acatttaaat     4140

gttaatagaa caaaatggtg gggcaatcat ttacattttt agggatatgt aattactagt     4200

tcaggtgtat tgccacaaga caaacatgtt aagaaacttt cccgttattt acgctctgtt     4260

cctgttaatc aacctctgga ttacaaaatt tgtgaaagat tgactgatat tcttaactat     4320

gttgctcctt ttacgctgtg tggatatgct gctttatagc ctctgtatct agctattgct     4380

tcccgtacgg ctttcgtttt ctcctccttg tataaatcct ggttgctgtc tcttttagag     4440

gagttgtggc ccgttgtccg tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc     4500

cccactggct ggggcattgc caccacctgt caactccttt ctgggacttt cgctttcccc     4560

ctcccgatcg ccacggcaga actcatcgcc gcctgccttg cccgctgctg gacaggggct     4620

aggttgctgg gcactgataa ttccgtggtg ttgtcatcga attcggtacc tttttaaaag     4680

aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc ataacttcgt     4740

atagcataca ttatacgaag ttataattta tttgtgaaat ttgtgatgct attgctttat     4800

ttgtaaccat atgtttattt gtgaaatttg tgatgctatt gctttatttg taaccattgc     4860

tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct     4920

aactagggaa cccactgctt aagcctcaat aaagcttgcc tcgaccagcc tcgactgtgc     4980

cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag     5040

gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta     5100

ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag     5160

acaatagcag gcatgctggg gatgcggtgg gctctatggc ctgcagctgc attaatgaat     5220

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     5280

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     5340

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     5400

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     5460

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     5520

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     5580

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     5640

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5700

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5760

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     5820

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     5880

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     5940

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     6000

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     6060

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     6120

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     6180

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     6240

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     6300

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     6360

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     6420

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     6480

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     6540

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     6600

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     6660

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     6720

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     6780

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     6840

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     6900

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     6960

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     7020

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     7080

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     7140

g                                                                     7141


<210>  63
<211>  7264
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_T7_scFvCD19-DmrA-mCherry lentiviral vector

<400>  63
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat       60

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc      120

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg      180

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc      240

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt      300

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca      360

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa      420

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac      480

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct      540

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact      600

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt      660

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa      720

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa      780

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca      840

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata      900

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag      960

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     1020

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     1080

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     1140

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     1200

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     1260

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     1320

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     1380

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     1440

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     1500

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     1560

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     1620

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     1680

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     1740

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     1800

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact     1860

tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata     1920

gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc     1980

gattacgcgt aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2040

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2100

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2160

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2220

acctgcaggt aatacgactc actatagggt ccactgccgc caccatgccc ctgggcctgc     2280

tgtggctggg cctggccctg ctgggcgccc tgcacgccca ggccggatcc gatatccaga     2340

tgacccagac caccagcagc ctgagcgcca gcctgggcga tagagtgacc atcagctgca     2400

gagccagcca ggacatcagc aagtacctga actggtatca gcagaaaccc gacggcaccg     2460

tgaagctgct gatctaccac accagcagac tgcacagcgg cgtgcccagc agattttctg     2520

gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag gaagatatcg     2580

ctacctactt ctgtcagcaa ggcaacaccc tgccctacac cttcggcgga ggcaccaagc     2640

tggaaatcac cggcagcaca agcggcagcg gcaagcctgg atctggcgag ggaagcacca     2700

agggcgaagt gaaactgcag gaaagcggcc ctggactggt ggccccaagc cagtctctga     2760

gcgtgacctg taccgtgtcc ggcgtgtccc tgcctgacta tggcgtgtcc tggatcagac     2820

agccacccag aaagggcctg gaatggctgg gagtgatctg gggcagcgag acaacctact     2880

acaacagcgc cctgaagtcc cggctgacca tcatcaagga caactccaag agccaggtgt     2940

tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc gccaagcact     3000

actactacgg cggcagctac gccatggact actggggcca gggcacaagc gtgaccgtgt     3060

ccagcgctag cggcggaggt gggagcggag tgcaggtgga aaccatctcc ccaggagacg     3120

ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag     3180

atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca     3240

agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag     3300

ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc     3360

caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc ggccgctccg     3420

gtgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccg ggcccctcta     3480

gagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg     3540

tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc     3600

gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct     3660

tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc     3720

ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg     3780

tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctct ctgcaggacg     3840

gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa     3900

tgcagaagaa gactatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg     3960

ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg     4020

aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca     4080

acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac     4140

gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa gtcgaccgag     4200

catcttaccg ccatttatac ccatatttgt tctgtttttc ttgatttggg tatacattta     4260

aatgttaata gaacaaaatg gtggggcaat catttacatt tttagggata tgtaattact     4320

agttcaggtg tattgccaca agacaaacat gttaagaaac tttcccgtta tttacgctct     4380

gttcctgtta atcaacctct ggattacaaa atttgtgaaa gattgactga tattcttaac     4440

tatgttgctc cttttacgct gtgtggatat gctgctttat agcctctgta tctagctatt     4500

gcttcccgta cggctttcgt tttctcctcc ttgtataaat cctggttgct gtctctttta     4560

gaggagttgt ggcccgttgt ccgtcaacgt ggcgtggtgt gctctgtgtt tgctgacgca     4620

acccccactg gctggggcat tgccaccacc tgtcaactcc tttctgggac tttcgctttc     4680

cccctcccga tcgccacggc agaactcatc gccgcctgcc ttgcccgctg ctggacaggg     4740

gctaggttgc tgggcactga taattccgtg gtgttgtcat cgaattcggt acctttttaa     4800

aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atcataactt     4860

cgtatagcat acattatacg aagttataat ttatttgtga aatttgtgat gctattgctt     4920

tatttgtaac catatgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat     4980

tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg     5040

gctaactagg gaacccactg cttaagcctc aataaagctt gcctcgacca gcctcgactg     5100

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg     5160

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga     5220

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg     5280

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcctgcagc tgcattaatg     5340

aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct     5400

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     5460

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     5520

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     5580

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     5640

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     5700

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     5760

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     5820

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     5880

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     5940

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     6000

tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     6060

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     6120

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     6180

gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     6240

aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     6300

atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     6360

gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     6420

acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     6480

ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     6540

tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     6600

ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg     6660

ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     6720

atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     6780

taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     6840

catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     6900

atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     6960

acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     7020

aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     7080

ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     7140

cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     7200

atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     7260

ttag                                                                  7264


<210>  64
<211>  740
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8ss-DmrC-CD8TM-41BB-CD3z-P2A-IgKss-CD19scFv-DmrA-CD4TM protein

<400>  64

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser Ile Leu Trp His Glu Met Trp His Glu 
            20                  25                  30          


Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys 
        35                  40                  45              


Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly 
    50                  55                  60                  


Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp 
65                  70                  75                  80  


Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn 
                85                  90                  95      


Val Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg 
            100                 105                 110         


Arg Ile Ser Lys Ala Ser Ala Gly Thr Gly Ser Asp Ile Tyr Ile Trp 
        115                 120                 125             


Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile 
    130                 135                 140                 


Thr Met His Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
145                 150                 155                 160 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                165                 170                 175     


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            180                 185                 190         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        195                 200                 205             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    210                 215                 220                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
225                 230                 235                 240 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                245                 250                 255     


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            260                 265                 270         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        275                 280                 285             


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ser Gly Ser 
    290                 295                 300                 


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
305                 310                 315                 320 


Asn Pro Gly Pro Ser Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu 
                325                 330                 335     


Leu Leu Trp Val Pro Gly Ser Thr Gly Ser Asp Ile Gln Met Thr Gln 
            340                 345                 350         


Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser 
        355                 360                 365             


Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln 
    370                 375                 380                 


Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu 
385                 390                 395                 400 


His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                405                 410                 415     


Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr 
            420                 425                 430         


Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr 
        435                 440                 445             


Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser 
    450                 455                 460                 


Gly Glu Gly Ser Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro 
465                 470                 475                 480 


Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser 
                485                 490                 495     


Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro 
            500                 505                 510         


Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr 
        515                 520                 525             


Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn 
    530                 535                 540                 


Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 
545                 550                 555                 560 


Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr 
                565                 570                 575     


Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala 
            580                 585                 590         


Ser Gly Gly Gly Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly 
        595                 600                 605             


Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr 
    610                 615                 620                 


Thr Gly Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg 
625                 630                 635                 640 


Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly 
                645                 650                 655     


Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu 
            660                 665                 670         


Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile 
        675                 680                 685             


Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu 
    690                 695                 700                 


Glu Gly Gly Arg Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu 
705                 710                 715                 720 


Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe Cys Val Arg Cys Arg His 
                725                 730                 735     


Arg Arg Arg Gln 
            740 


<210>  65
<211>  2223
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8ss-DmrC-CD8TM-41BB-CD3z-P2A-IgKss-CD19scFv-DmrA-CD4TM DNA

<400>  65
atggctctgc ctgtgacagc tctgctgctg cctctggccc tgctgctcca tgccgccaga       60

cccggatcca tcctctggca tgagatgtgg catgaaggcc tggaagaggc atctcgtttg      120

tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt gcatgctatg      180

atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta tggtcgagat      240

ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt caaggacctc      300

ctccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaaggc tagcgccggc      360

actggttccg acatctacat ctgggcccct ctggccggca catgtggcgt gctgctgctg      420

agcctcgtga tcaccatgca taaacggggc agaaagaaac tcctgtatat attcaaacaa      480

ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca      540

gaagaagaag aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct      600

gcctaccagc agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag      660

tacgacgtcc tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg      720

aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac      780

agcgagatcg gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag      840

ggcctgtcca ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca      900

aggtcaggat ctggcgccac gaacttctct ctgttaaagc aagcaggaga tgttgaagaa      960

aaccccgggc cttcaatgga gacagacaca ctcctgctat gggtactgct gctctgggtt     1020

ccaggttcca ctggttccga tatccagatg acccagacca ccagcagcct gagcgccagc     1080

ctgggcgata gagtgaccat cagctgcaga gccagccagg acatcagcaa gtacctgaac     1140

tggtatcagc agaaacccga cggcaccgtg aagctgctga tctaccacac cagcagactg     1200

cacagcggcg tgcccagcag attttctggc agcggctccg gcaccgacta cagcctgacc     1260

atctccaacc tggaacagga agatatcgct acctacttct gtcagcaagg caacaccctg     1320

ccctacacct tcggcggagg caccaagctg gaaatcaccg gcagcacaag cggcagcggc     1380

aagcctggat ctggcgaggg aagcaccaag ggcgaagtga aactgcagga aagcggccct     1440

ggactggtgg ccccaagcca gtctctgagc gtgacctgta ccgtgtccgg cgtgtccctg     1500

cctgactatg gcgtgtcctg gatcagacag ccacccagaa agggcctgga atggctggga     1560

gtgatctggg gcagcgagac aacctactac aacagcgccc tgaagtcccg gctgaccatc     1620

atcaaggaca actccaagag ccaggtgttc ctgaagatga acagcctgca gaccgacgac     1680

accgccatct actactgcgc caagcactac tactacggcg gcagctacgc catggactac     1740

tggggccagg gcacaagcgt gaccgtgtcc agcgctagcg gcggaggtgg gagcggagtg     1800

caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc     1860

gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga     1920

aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg     1980

gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat     2040

ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag     2100

cttctaaaac tggaaggcgg ccgcatggcc ctgattgtgc tggggggcgt cgccggcctc     2160

ctgcttttca ttgggctagg catcttcttc tgtgtcaggt gccggcaccg aaggcgccaa     2220

taa                                                                   2223


<210>  66
<211>  7651
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_MND-CD8ss-DmrC-CD8TM-41BB-CD3z-P2A-IgKss-CD19scFv-DmrA-CD4TM
       lentiviral vector

<400>  66
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg      240

gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc      300

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      360

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      420

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      480

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      540

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      600

attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta      660

aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta      720

gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga      780

tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg      840

atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt      900

aagaccaccg cacagcaagc ggccctgatc ttcagacctg gaggaggaga tatgagggac      960

aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca     1020

cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct     1080

ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg     1140

acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg     1200

gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag     1260

gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt     1320

tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaatgaa     1380

tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat     1440

tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa     1500

caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat     1560

tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata     1620

gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt     1680

cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt     1740

ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta     1800

acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata     1860

atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt     1920

atcgattacg cgtgtacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa     1980

gcagttcctg ccccggctca gggccaagaa cagttggaac agcagaatat gggccaaaca     2040

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga     2100

tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg     2160

acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt     2220

cgcgcgcttc tgctccccga gctctatata agcagagctc gtttagtgaa ccgtcagatc     2280

cctgcagggc cgccaccatg gctctgcctg tgacagctct gctgctgcct ctggccctgc     2340

tgctccatgc cgccagaccc ggatccatcc tctggcatga gatgtggcat gaaggcctgg     2400

aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg     2460

agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc     2520

aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag     2580

ggaatgtcaa ggacctcctc caagcctggg acctctatta tcatgtgttc cgacgaatct     2640

caaaggctag cgccggcact ggttccgaca tctacatctg ggcccctctg gccggcacat     2700

gtggcgtgct gctgctgagc ctcgtgatca ccatgcataa acggggcaga aagaaactcc     2760

tgtatatatt caaacaacca tttatgagac cagtacaaac tactcaagag gaagatggct     2820

gtagctgccg atttccagaa gaagaagaag gaggatgtga actgcgggtg aagttcagca     2880

gaagcgccga cgcccctgcc taccagcagg gccagaatca gctgtacaac gagctgaacc     2940

tgggcagaag ggaagagtac gacgtcctgg ataagcggag aggccgggac cctgagatgg     3000

gcggcaagcc tcggcggaag aacccccagg aaggcctgta taacgaactg cagaaagaca     3060

agatggccga ggcctacagc gagatcggca tgaagggcga gcggaggcgg ggcaagggcc     3120

acgacggcct gtatcagggc ctgtccaccg ccaccaagga tacctacgac gccctgcaca     3180

tgcaggccct gcccccaagg tcaggatctg gcgccacgaa cttctctctg ttaaagcaag     3240

caggagatgt tgaagaaaac cccgggcctt caatggagac agacacactc ctgctatggg     3300

tactgctgct ctgggttcca ggttccactg gttccgatat ccagatgacc cagaccacca     3360

gcagcctgag cgccagcctg ggcgatagag tgaccatcag ctgcagagcc agccaggaca     3420

tcagcaagta cctgaactgg tatcagcaga aacccgacgg caccgtgaag ctgctgatct     3480

accacaccag cagactgcac agcggcgtgc ccagcagatt ttctggcagc ggctccggca     3540

ccgactacag cctgaccatc tccaacctgg aacaggaaga tatcgctacc tacttctgtc     3600

agcaaggcaa caccctgccc tacaccttcg gcggaggcac caagctggaa atcaccggca     3660

gcacaagcgg cagcggcaag cctggatctg gcgagggaag caccaagggc gaagtgaaac     3720

tgcaggaaag cggccctgga ctggtggccc caagccagtc tctgagcgtg acctgtaccg     3780

tgtccggcgt gtccctgcct gactatggcg tgtcctggat cagacagcca cccagaaagg     3840

gcctggaatg gctgggagtg atctggggca gcgagacaac ctactacaac agcgccctga     3900

agtcccggct gaccatcatc aaggacaact ccaagagcca ggtgttcctg aagatgaaca     3960

gcctgcagac cgacgacacc gccatctact actgcgccaa gcactactac tacggcggca     4020

gctacgccat ggactactgg ggccagggca caagcgtgac cgtgtccagc gctagcggcg     4080

gaggtgggag cggagtgcag gtggaaacca tctccccagg agacgggcgc accttcccca     4140

agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaatttg     4200

attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc     4260

gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat     4320

ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca catgccactc     4380

tcgtcttcga tgtggagctt ctaaaactgg aaggcggccg catggccctg attgtgctgg     4440

ggggcgtcgc cggcctcctg cttttcattg ggctaggcat cttcttctgt gtcaggtgcc     4500

ggcaccgaag gcgccaataa gtcgaccgag catcttaccg ccatttatac ccatatttgt     4560

tctgtttttc ttgatttggg tatacattta aatgttaata gaacaaaatg gtggggcaat     4620

catttacatt tttagggata tgtaattact agttcaggtg tattgccaca agacaaacat     4680

gttaagaaac tttcccgtta tttacgctct gttcctgtta atcaacctct ggattacaaa     4740

atttgtgaaa gattgactga tattcttaac tatgttgctc cttttacgct gtgtggatat     4800

gctgctttat agcctctgta tctagctatt gcttcccgta cggctttcgt tttctcctcc     4860

ttgtataaat cctggttgct gtctctttta gaggagttgt ggcccgttgt ccgtcaacgt     4920

ggcgtggtgt gctctgtgtt tgctgacgca acccccactg gctggggcat tgccaccacc     4980

tgtcaactcc tttctgggac tttcgctttc cccctcccga tcgccacggc agaactcatc     5040

gccgcctgcc ttgcccgctg ctggacaggg gctaggttgc tgggcactga taattccgtg     5100

gtgttgtcat cgaattcggt acctttttaa aagaaaaggg gggactggaa gggctaattc     5160

actcccaacg aagacaagat atcataactt cgtatagcat acattatacg aagttataat     5220

ttatttgtga aatttgtgat gctattgctt tatttgtaac catatgttta tttgtgaaat     5280

ttgtgatgct attgctttat ttgtaaccat tgctttttgc ttgtactggg tctctctggt     5340

tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc     5400

aataaagctt gcctcgacca gcctcgactg tgccttctag ttgccagcca tctgttgttt     5460

gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat     5520

aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg     5580

tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg     5640

tgggctctat ggcctgcagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt     5700

gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     5760

gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     5820

taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc     5880

cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg     5940

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     6000

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     6060

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     6120

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     6180

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     6240

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     6300

cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct     6360

gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac     6420

cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc     6480

tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg     6540

ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta     6600

aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca     6660

atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc     6720

ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc     6780

tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc     6840

agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat     6900

taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt     6960

tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc     7020

cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag     7080

ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt     7140

tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac     7200

tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg     7260

cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat     7320

tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc     7380

gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc     7440

tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa     7500

atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg     7560

tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     7620

cacatttccc cgaaaagtgc cacctgacgt c                                    7651


<210>  67
<211>  739
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8ss-DmrC-CD8TM-41BB-CD3z-P2A-IgKss-CD19scFv-DmrA-CD4TM codon 
       optimized protein

<400>  67

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser Ile Leu Trp His Glu Met Trp His Glu 
            20                  25                  30          


Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys 
        35                  40                  45              


Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly 
    50                  55                  60                  


Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp 
65                  70                  75                  80  


Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn 
                85                  90                  95      


Val Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg 
            100                 105                 110         


Arg Ile Ser Lys Ala Ser Ala Gly Thr Gly Ser Asp Ile Tyr Ile Trp 
        115                 120                 125             


Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile 
    130                 135                 140                 


Thr Met His Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
145                 150                 155                 160 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                165                 170                 175     


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            180                 185                 190         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        195                 200                 205             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    210                 215                 220                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
225                 230                 235                 240 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                245                 250                 255     


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            260                 265                 270         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        275                 280                 285             


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ser Gly Ser 
    290                 295                 300                 


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
305                 310                 315                 320 


Asn Pro Gly Pro Ser Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu 
                325                 330                 335     


Leu Leu Trp Val Pro Gly Ser Thr Gly Asp Ile Gln Met Thr Gln Thr 
            340                 345                 350         


Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys 
        355                 360                 365             


Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys 
    370                 375                 380                 


Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His 
385                 390                 395                 400 


Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr 
                405                 410                 415     


Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe 
            420                 425                 430         


Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys 
        435                 440                 445             


Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly 
    450                 455                 460                 


Glu Gly Ser Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
465                 470                 475                 480 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                485                 490                 495     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            500                 505                 510         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        515                 520                 525             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    530                 535                 540                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
545                 550                 555                 560 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                565                 570                 575     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Pro Arg 
            580                 585                 590         


Gly Gly Gly Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp 
        595                 600                 605             


Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr 
    610                 615                 620                 


Gly Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn 
625                 630                 635                 640 


Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp 
                645                 650                 655     


Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr 
            660                 665                 670         


Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile 
        675                 680                 685             


Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
    690                 695                 700                 


Gly Gly Arg Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu 
705                 710                 715                 720 


Leu Phe Ile Gly Leu Gly Ile Phe Phe Cys Val Arg Cys Arg His Arg 
                725                 730                 735     


Arg Arg Gln 
            


<210>  68
<211>  2220
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8ss-DmrC-CD8TM-41BB-CD3z-P2A-IgKss-CD19scFv-DmrA-CD4TM codon 
       optimized DNA

<400>  68
atggccctcc ctgtgaccgc cctgctgctc cccctcgccc tgttgctcca tgctgcccga       60

cctggatcca tcctttggca cgagatgtgg cacgagggac tcgaagaagc gtcccggctg      120

tacttcggag agcggaacgt gaaggggatg ttcgaagtgc tggaacccct gcacgccatg      180

atggagcggg gtcctcagac ccttaaagaa acaagcttca accaggcgta cgggcgcgac      240

ctgatggaag cccaggagtg gtgccgcaag tacatgaagt ccggaaacgt gaaggatctg      300

ctgcaagcct gggatctgta ctaccacgtg ttcagaagga tctcaaaggc tagcgccggc      360

actggttcgg atatctacat ttgggcaccg ctcgccggca cttgtggagt gctgttgctg      420

tccctcgtga tcaccatgca taagagggga cggaagaagc tgctgtacat tttcaagcag      480

ccattcatgc ggcctgtgca aaccacccag gaggaggacg ggtgcagctg ccggttccct      540

gaggaagagg agggcggatg cgaactgcgc gtgaagttca gccggagcgc agatgctccc      600

gcataccaac agggacagaa ccagctgtat aacgagctga acctgggcag aagggaagag      660

tacgacgtcc tcgacaagcg gcggggacgc gacccagaaa tgggaggaaa gccccgccgg      720

aagaacccgc aggaaggcct gtacaacgag ttgcagaaag acaagatggc tgaagcttac      780

tcggagattg gcatgaaggg ggagagaaga agagggaagg gccacgacgg cctttaccaa      840

ggactgagca ctgccaccaa ggacacctac gatgcgctgc acatgcaggc cctgcccccg      900

cggtccggtt cgggcgcgac taacttcagc ctgctgaagc aggccggaga tgtggaggaa      960

aaccctggac cgtccatgga gactgatacc ctgcttctgt gggtcctgct cctctgggtg     1020

ccgggctcca ccggtgacat ccagatgacc cagaccacct catccctgag cgcctctctg     1080

ggtgatcgcg tgactatctc ctgccgggcg tcgcaggata tctccaagta cctgaactgg     1140

taccagcaaa aaccggacgg gaccgtgaaa ctgctgatct accatacttc ccgccttcat     1200

tccggagtgc cctcccggtt ttccggctcg ggttcaggga ctgattattc gctgaccatt     1260

tccaacctgg agcaggagga cattgcgacc tacttctgcc aacaaggaaa caccctgccc     1320

tacactttcg gtggtggaac caagctcgag atcaccggat caacctcggg cagcgggaag     1380

ccgggcagcg gagagggatc gacgaaagga gaagtcaagc tgcaggaatc cggcccggga     1440

ctggtggccc cgagccagtc gctctccgtc acttgcaccg tgtcgggagt gtccttgccc     1500

gactacggag tgtcatggat tcggcagcca cctcgcaagg gcctggaatg gctcggcgtg     1560

atttggggct cagaaaccac atactacaac agcgccctga agtctcggct caccatcatc     1620

aaggacaatt ccaagtccca agtgttcctg aagatgaata gcttgcagac tgacgacacc     1680

gcgatctact actgtgccaa gcactactac tacggcggtt cctacgccat ggactactgg     1740

ggacaaggaa cttccgtgac tgtctcctcc cctagggggg gtggtggttc gggggtccag     1800

gtggaaacca tttcccccgg cgacgggcgc accttcccga agcgcggaca gacctgtgtg     1860

gtgcactata ccggaatgct cgaagatgga aagaagtttg acagctccag ggaccgcaac     1920

aagcctttca agtttatgct tggaaagcag gaagtcatcc ggggctggga agagggagtc     1980

gcccagatga gcgtcggcca gcgggccaag ctgacgatct cccctgacta tgcctacggc     2040

gctaccggcc atcccggaat cattccgccg cacgcaaccc tcgtgttcga cgtggaattg     2100

ctcaagctgg aaggcggccg catggcgctg atagtgctcg gcggagtggc cggactgctg     2160

ctgttcatcg gcctgggcat cttcttctgc gtgagatgcc gccatagaag gcggcaatga     2220


<210>  69
<211>  7648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL-MND-CD8ss-DmrC-CD8TM-41BB-Zeta-P2A-IgKss-CD19scFv-DmrA-CD4TM
       codon optimized lentiviral vector

<400>  69
taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca       60

taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt      120

ttatcgatta cgcgtgtaca gagagacagc agaatatggg ccaaacagga tatctgtggt      180

aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat atgggccaaa      240

caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca      300

gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa      360

ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg      420

ttcgcgcgct tctgctcccc gagctctata taagcagagc tcgtttagtg aaccgtcaga      480

tccctgcagg gccgccacca tggccctccc tgtgaccgcc ctgctgctcc ccctcgccct      540

gttgctccat gctgcccgac ctggatccat cctttggcac gagatgtggc acgagggact      600

cgaagaagcg tcccggctgt acttcggaga gcggaacgtg aaggggatgt tcgaagtgct      660

ggaacccctg cacgccatga tggagcgggg tcctcagacc cttaaagaaa caagcttcaa      720

ccaggcgtac gggcgcgacc tgatggaagc ccaggagtgg tgccgcaagt acatgaagtc      780

cggaaacgtg aaggatctgc tgcaagcctg ggatctgtac taccacgtgt tcagaaggat      840

ctcaaaggct agcgccggca ctggttcgga tatctacatt tgggcaccgc tcgccggcac      900

ttgtggagtg ctgttgctgt ccctcgtgat caccatgcat aagaggggac ggaagaagct      960

gctgtacatt ttcaagcagc cattcatgcg gcctgtgcaa accacccagg aggaggacgg     1020

gtgcagctgc cggttccctg aggaagagga gggcggatgc gaactgcgcg tgaagttcag     1080

ccggagcgca gatgctcccg cataccaaca gggacagaac cagctgtata acgagctgaa     1140

cctgggcaga agggaagagt acgacgtcct cgacaagcgg cggggacgcg acccagaaat     1200

gggaggaaag ccccgccgga agaacccgca ggaaggcctg tacaacgagt tgcagaaaga     1260

caagatggct gaagcttact cggagattgg catgaagggg gagagaagaa gagggaaggg     1320

ccacgacggc ctttaccaag gactgagcac tgccaccaag gacacctacg atgcgctgca     1380

catgcaggcc ctgcccccgc ggtccggttc gggcgcgact aacttcagcc tgctgaagca     1440

ggccggagat gtggaggaaa accctggacc gtccatggag actgataccc tgcttctgtg     1500

ggtcctgctc ctctgggtgc cgggctccac cggtgacatc cagatgaccc agaccacctc     1560

atccctgagc gcctctctgg gtgatcgcgt gactatctcc tgccgggcgt cgcaggatat     1620

ctccaagtac ctgaactggt accagcaaaa accggacggg accgtgaaac tgctgatcta     1680

ccatacttcc cgccttcatt ccggagtgcc ctcccggttt tccggctcgg gttcagggac     1740

tgattattcg ctgaccattt ccaacctgga gcaggaggac attgcgacct acttctgcca     1800

acaaggaaac accctgccct acactttcgg tggtggaacc aagctcgaga tcaccggatc     1860

aacctcgggc agcgggaagc cgggcagcgg agagggatcg acgaaaggag aagtcaagct     1920

gcaggaatcc ggcccgggac tggtggcccc gagccagtcg ctctccgtca cttgcaccgt     1980

gtcgggagtg tccttgcccg actacggagt gtcatggatt cggcagccac ctcgcaaggg     2040

cctggaatgg ctcggcgtga tttggggctc agaaaccaca tactacaaca gcgccctgaa     2100

gtctcggctc accatcatca aggacaattc caagtcccaa gtgttcctga agatgaatag     2160

cttgcagact gacgacaccg cgatctacta ctgtgccaag cactactact acggcggttc     2220

ctacgccatg gactactggg gacaaggaac ttccgtgact gtctcctccc ctaggggggg     2280

tggtggttcg ggggtccagg tggaaaccat ttcccccggc gacgggcgca ccttcccgaa     2340

gcgcggacag acctgtgtgg tgcactatac cggaatgctc gaagatggaa agaagtttga     2400

cagctccagg gaccgcaaca agcctttcaa gtttatgctt ggaaagcagg aagtcatccg     2460

gggctgggaa gagggagtcg cccagatgag cgtcggccag cgggccaagc tgacgatctc     2520

ccctgactat gcctacggcg ctaccggcca tcccggaatc attccgccgc acgcaaccct     2580

cgtgttcgac gtggaattgc tcaagctgga aggcggccgc atggcgctga tagtgctcgg     2640

cggagtggcc ggactgctgc tgttcatcgg cctgggcatc ttcttctgcg tgagatgccg     2700

ccatagaagg cggcaatgag tcgaccgagc atcttaccgc catttatacc catatttgtt     2760

ctgtttttct tgatttgggt atacatttaa atgttaatag aacaaaatgg tggggcaatc     2820

atttacattt ttagggatat gtaattacta gttcaggtgt attgccacaa gacaaacatg     2880

ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg gattacaaaa     2940

tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg tgtggatatg     3000

ctgctttata gcctctgtat ctagctattg cttcccgtac ggctttcgtt ttctcctcct     3060

tgtataaatc ctggttgctg tctcttttag aggagttgtg gcccgttgtc cgtcaacgtg     3120

gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt gccaccacct     3180

gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca gaactcatcg     3240

ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat aattccgtgg     3300

tgttgtcatc gaattcggta cctttttaaa agaaaagggg ggactggaag ggctaattca     3360

ctcccaacga agacaagata tcataacttc gtatagcata cattatacga agttataatt     3420

tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atatgtttat ttgtgaaatt     3480

tgtgatgcta ttgctttatt tgtaaccatt gctttttgct tgtactgggt ctctctggtt     3540

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca     3600

ataaagcttg cctcgaccag cctcgactgt gccttctagt tgccagccat ctgttgtttg     3660

cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata     3720

aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt     3780

ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt     3840

gggctctatg gcctgcagct gcattaatga atcggccaac gcgcggggag aggcggtttg     3900

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg     3960

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat     4020

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc     4080

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     4140

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     4200

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     4260

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     4320

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     4380

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     4440

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     4500

ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg     4560

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     4620

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     4680

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     4740

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     4800

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     4860

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     4920

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     4980

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     5040

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     5100

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     5160

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     5220

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     5280

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     5340

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     5400

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     5460

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     5520

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     5580

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     5640

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     5700

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt     5760

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc     5820

acatttcccc gaaaagtgcc acctgacgtc aatgtagtct tatgcaatac tcttgtagtc     5880

ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc     5940

atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg     6000

tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg     6060

cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc     6120

tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt     6180

agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc     6240

agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca     6300

gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg     6360

cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg     6420

tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta     6480

aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta     6540

gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg     6600

ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca     6660

gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta     6720

gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccctgatc     6780

ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa     6840

gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag     6900

agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga     6960

agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt     7020

atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa     7080

ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta     7140

aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct     7200

gtgccttgga atgctagttg gagtaatgaa tctctggaac agatttggaa tcacacgacc     7260

tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa     7320

gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca     7380

agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg     7440

atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga     7500

gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc     7560

gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga     7620

ttagtgaacg gatctcgacg gtatcggt                                        7648


<210>  70
<211>  762
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS-DmrC-CD8TM-41BB-CD3z-P2A-SS-CD123scFv-DmrA-CD4TM protein

<400>  70

Met Arg Pro Thr Trp Ala Trp Trp Leu Phe Leu Val Leu Leu Leu Ala 
1               5                   10                  15      


Leu Trp Ala Pro Ala Arg Gly Gly Ser Ile Leu Trp His Glu Met Trp 
            20                  25                  30          


His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn 
        35                  40                  45              


Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu 
    50                  55                  60                  


Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly 
65                  70                  75                  80  


Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser 
                85                  90                  95      


Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val 
            100                 105                 110         


Phe Arg Arg Ile Ser Lys Ala Ser Ala Gly Thr Gly Ser Asp Ile Tyr 
        115                 120                 125             


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
    130                 135                 140                 


Val Ile Thr Met His Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
145                 150                 155                 160 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                165                 170                 175     


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
            180                 185                 190         


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
        195                 200                 205             


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    210                 215                 220                 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
225                 230                 235                 240 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                245                 250                 255     


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            260                 265                 270         


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        275                 280                 285             


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly 
    290                 295                 300                 


Gly Arg Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala 
305                 310                 315                 320 


Gly Asp Val Glu Glu Asn Pro Gly Pro Ser Leu Trp Trp Arg Leu Trp 
                325                 330                 335     


Trp Leu Leu Leu Leu Leu Leu Leu Leu Trp Pro Met Val Trp Ala Pro 
            340                 345                 350         


Arg Ala Asp Tyr Lys Asp Ile Val Met Thr Gln Ser His Lys Phe Met 
        355                 360                 365             


Ser Thr Ser Val Gly Asp Arg Val Asn Ile Thr Cys Lys Ala Ser Gln 
    370                 375                 380                 


Asn Val Asp Ser Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser 
385                 390                 395                 400 


Pro Lys Ala Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro 
                405                 410                 415     


Asp Arg Phe Thr Gly Arg Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
            420                 425                 430         


Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln Tyr 
        435                 440                 445             


Tyr Ser Thr Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
    450                 455                 460                 


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
465                 470                 475                 480 


Gly Gly Gly Gly Ser Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu 
                485                 490                 495     


Val Gln Pro Gly Gly Ser Leu Ser Leu Ser Cys Ala Ala Ser Gly Phe 
            500                 505                 510         


Thr Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Pro Pro Gly Lys 
        515                 520                 525             


Ala Leu Glu Trp Leu Ala Leu Ile Arg Ser Lys Ala Asp Gly Tyr Thr 
    530                 535                 540                 


Thr Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Leu Ser Arg Asp 
545                 550                 555                 560 


Asp Ser Gln Ser Ile Leu Tyr Leu Gln Met Asn Ala Leu Arg Pro Glu 
                565                 570                 575     


Asp Ser Ala Thr Tyr Tyr Cys Ala Arg Asp Ala Ala Tyr Tyr Ser Tyr 
            580                 585                 590         


Tyr Ser Pro Glu Gly Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
        595                 600                 605             


Thr Val Ser Ser Ser Ala Ser Gly Gly Gly Gly Ser Gly Val Gln Val 
    610                 615                 620                 


Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln 
625                 630                 635                 640 


Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Phe 
                645                 650                 655     


Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys 
            660                 665                 670         


Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val 
        675                 680                 685             


Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala 
    690                 695                 700                 


Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp 
705                 710                 715                 720 


Val Glu Leu Leu Lys Leu Glu Gly Gly Arg Met Ala Leu Ile Val Leu 
                725                 730                 735     


Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe 
            740                 745                 750         


Cys Val Arg Cys Arg His Arg Arg Arg Gln 
        755                 760         


<210>  71
<211>  2289
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS-DmrC-CD8TM-41BB-CD3z-P2A-SS-CD123scFv-DmrA-CD4TM DNA

<400>  71
atgcgcccca cctgggcctg gtggctgttc ctggtgctgc tgctggccct gtgggcaccc       60

gctcgcggcg gatccatcct ctggcatgag atgtggcatg aaggcctgga agaggcatct      120

cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat      180

gctatgatgg aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt      240

cgagatttaa tggaggccca agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag      300

gacctcctcc aagcctggga cctctattat catgtgttcc gacgaatctc aaaggctagc      360

gccggcactg gttccgacat ctacatctgg gcccctctgg ccggcacatg tggcgtgctg      420

ctgctgagcc tcgtgatcac catgcataaa cggggcagaa agaaactcct gtatatattc      480

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga      540

tttccagaag aagaagaagg aggatgtgaa ctgcgggtga agttcagcag aagcgccgac      600

gcccctgcct accagcaggg ccagaatcag ctgtacaacg agctgaacct gggcagaagg      660

gaagagtacg acgtcctgga taagcggaga ggccgggacc ctgagatggg cggcaagcct      720

cggcggaaga acccccagga aggcctgtat aacgaactgc agaaagacaa gatggccgag      780

gcctacagcg agatcggcat gaagggcgag cggaggcggg gcaagggcca cgacggcctg      840

tatcagggcc tgtccaccgc caccaaggat acctacgacg ccctgcacat gcaggccctg      900

cccccaaggg gcggccgctc aggatctggc gccacgaact tctctctgtt aaagcaagca      960

ggagatgttg aagaaaaccc cgggccttca ctgtggtggc gcctgtggtg gctgctcctg     1020

cttctgttgc tcctgtggcc catggtgtgg gcccctaggg cggactacaa agatattgtg     1080

atgacccagt ctcacaaatt catgtccaca tcagtaggag acagggtcaa catcacctgc     1140

aaggccagtc agaatgtgga tagtgctgta gcctggtatc aacagaaacc agggcaatct     1200

cctaaagcac tgatttactc ggcatcctac cggtacagtg gagtccctga tcgcttcaca     1260

ggcaggggat ctgggacaga tttcactctc accatcagca gtgtgcaggc tgaagacctg     1320

gcagtttatt actgtcagca atattatagc actccgtgga cgttcggtgg aggcaccaag     1380

ctggaaatca aacgtggtgg tggtggttct ggtggtggtg gttctggcgg cggcggctcc     1440

ggtggtggtg gatccgaggt gaagctggtg gagtctggag gaggcttggt acagcctggg     1500

ggttctctga gtctctcctg tgcagcttct ggattcacct tcactgatta ctacatgagc     1560

tgggtccgcc agcctccagg gaaggcactt gagtggttgg ctttgattag aagcaaagct     1620

gatggttaca caacagaata cagtgcatct gtgaagggtc ggttcaccct ctccagagat     1680

gattcccaaa gcatcctcta tcttcaaatg aatgccctga gacctgaaga cagtgccact     1740

tattactgtg caagagatgc ggcctactat agttactata gtcccgaggg ggctatggac     1800

tactggggtc aaggaacctc agtcaccgtc tcctcgagcg ctagcggcgg aggtgggagc     1860

ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag     1920

acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg     1980

gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa     2040

gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat     2100

gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat     2160

gtggagcttc taaaactgga aggcggccgc atggccctga ttgtgctggg gggcgtcgcc     2220

ggcctcctgc ttttcattgg gctaggcatc ttcttctgtg tcaggtgccg gcaccgaagg     2280

cgccaataa                                                             2289


<210>  72
<211>  7717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_MND-SS34-DmrC-CD8TM-41BB-CD3z-P2A-SS38-CD123scFv-DmrA-CD4TM 
       lentiviral vector

<400>  72
gtacagagag acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatccct gcagggccgc      360

caccatgcgc cccacctggg cctggtggct gttcctggtg ctgctgctgg ccctgtgggc      420

acccgctcgc ggcggatcca tcctctggca tgagatgtgg catgaaggcc tggaagaggc      480

atctcgtttg tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt      540

gcatgctatg atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta      600

tggtcgagat ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt      660

caaggacctc ctccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaaggc      720

tagcgccggc actggttccg acatctacat ctgggcccct ctggccggca catgtggcgt      780

gctgctgctg agcctcgtga tcaccatgca taaacggggc agaaagaaac tcctgtatat      840

attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg      900

ccgatttcca gaagaagaag aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc      960

cgacgcccct gcctaccagc agggccagaa tcagctgtac aacgagctga acctgggcag     1020

aagggaagag tacgacgtcc tggataagcg gagaggccgg gaccctgaga tgggcggcaa     1080

gcctcggcgg aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc     1140

cgaggcctac agcgagatcg gcatgaaggg cgagcggagg cggggcaagg gccacgacgg     1200

cctgtatcag ggcctgtcca ccgccaccaa ggatacctac gacgccctgc acatgcaggc     1260

cctgccccca aggggcggcc gctcaggatc tggcgccacg aacttctctc tgttaaagca     1320

agcaggagat gttgaagaaa accccgggcc ttcactgtgg tggcgcctgt ggtggctgct     1380

cctgcttctg ttgctcctgt ggcccatggt gtgggcccct agggcggact acaaagatat     1440

tgtgatgacc cagtctcaca aattcatgtc cacatcagta ggagacaggg tcaacatcac     1500

ctgcaaggcc agtcagaatg tggatagtgc tgtagcctgg tatcaacaga aaccagggca     1560

atctcctaaa gcactgattt actcggcatc ctaccggtac agtggagtcc ctgatcgctt     1620

cacaggcagg ggatctggga cagatttcac tctcaccatc agcagtgtgc aggctgaaga     1680

cctggcagtt tattactgtc agcaatatta tagcactccg tggacgttcg gtggaggcac     1740

caagctggaa atcaaacgtg gtggtggtgg ttctggtggt ggtggttctg gcggcggcgg     1800

ctccggtggt ggtggatccg aggtgaagct ggtggagtct ggaggaggct tggtacagcc     1860

tgggggttct ctgagtctct cctgtgcagc ttctggattc accttcactg attactacat     1920

gagctgggtc cgccagcctc cagggaaggc acttgagtgg ttggctttga ttagaagcaa     1980

agctgatggt tacacaacag aatacagtgc atctgtgaag ggtcggttca ccctctccag     2040

agatgattcc caaagcatcc tctatcttca aatgaatgcc ctgagacctg aagacagtgc     2100

cacttattac tgtgcaagag atgcggccta ctatagttac tatagtcccg agggggctat     2160

ggactactgg ggtcaaggaa cctcagtcac cgtctcctcg agcgctagcg gcggaggtgg     2220

gagcggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg     2280

ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc     2340

ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg     2400

ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga     2460

ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt     2520

cgatgtggag cttctaaaac tggaaggcgg ccgcatggcc ctgattgtgc tggggggcgt     2580

cgccggcctc ctgcttttca ttgggctagg catcttcttc tgtgtcaggt gccggcaccg     2640

aaggcgccaa taagtcgacc gagcatctta ccgccattta tacccatatt tgttctgttt     2700

ttcttgattt gggtatacat ttaaatgtta atagaacaaa atggtggggc aatcatttac     2760

atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa catgttaaga     2820

aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac aaaatttgtg     2880

aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga tatgctgctt     2940

tatagcctct gtatctagct attgcttccc gtacggcttt cgttttctcc tccttgtata     3000

aatcctggtt gctgtctctt ttagaggagt tgtggcccgt tgtccgtcaa cgtggcgtgg     3060

tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc acctgtcaac     3120

tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc atcgccgcct     3180

gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc gtggtgttgt     3240

catcgaattc ggtacctttt taaaagaaaa ggggggactg gaagggctaa ttcactccca     3300

acgaagacaa gatatcataa cttcgtatag catacattat acgaagttat aatttatttg     3360

tgaaatttgt gatgctattg ctttatttgt aaccatatgt ttatttgtga aatttgtgat     3420

gctattgctt tatttgtaac cattgctttt tgcttgtact gggtctctct ggttagacca     3480

gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag     3540

cttgcctcga ccagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc     3600

ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga     3660

ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca     3720

ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc     3780

tatggcctgc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     3840

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     3900

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     3960

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     4020

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     4080

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     4140

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     4200

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     4260

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     4320

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     4380

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     4440

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     4500

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     4560

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     4620

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     4680

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     4740

agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     4800

atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     4860

cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     4920

ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     4980

agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     5040

tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     5100

gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     5160

caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     5220

ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     5280

gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     5340

tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     5400

tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     5460

cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     5520

cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     5580

gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     5640

atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     5700

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     5760

ccccgaaaag tgccacctga cgtcaatgta gtcttatgca atactcttgt agtcttgcaa     5820

catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg     5880

attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac     5940

atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc     6000

tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta     6060

actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg     6120

tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg     6180

gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag     6240

ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga     6300

ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag     6360

agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca     6420

gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga     6480

ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag     6540

ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca     6600

accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag     6660

atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccct gatcttcaga     6720

cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta     6780

aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa     6840

aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact     6900

atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg     6960

cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca     7020

gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat     7080

caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct     7140

tggaatgcta gttggagtaa tgaatctctg gaacagattt ggaatcacac gacctggatg     7200

gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg     7260

caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg     7320

tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta     7380

ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg     7440

cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg     7500

cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg     7560

aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt     7620

gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa     7680

caaattacaa aaattcaaaa ttttatcgat tacgcgt                              7717


<210>  73
<211>  8230
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBB_REV-CMV-IgKss-CD19scFv-DmrA-CD4TM_FOR-MND-CD8ss-DmrC-CD8TM-41
       BB-CD3z lentiviral vector

<400>  73
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatcatat gccagcctat ggtgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      600

gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      660

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      720

caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag      780

cagagctcgt ttagtgaacc gggtctctct ggttagacca gatctgagcc tgggagctct      840

ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag      900

tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt      960

cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc     1020

agaggagatc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg     1080

gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagtagg     1140

gtgcgagagc gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa     1200

ggccaggggg aaagaaacaa tataaactaa aacatatagt tagggcaagc agggagctag     1260

aacgattcgc agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg     1320

gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacaa     1380

tagcagtcct ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag     1440

ataagataga ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca     1500

caggaaacaa cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa     1560

tggtacatca ggccatatca cctagaactt taaattaaga cagcagtaca aatggcagta     1620

ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata     1680

gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt     1740

caaaattttc gggtttatta cagggacagc agagatccag tttggaaagg accagcaaag     1800

ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat aaaagtagtg     1860

ccaagaagaa aagcaaagat catcagggat tatggaaaac agatggcagg tgatgattgt     1920

gtggcaagta gacaggatga ggattaacac atggaaaaga ttagtaaaac accatagctc     1980

tagagcgatc ccgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga     2040

attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa     2100

gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt     2160

cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag     2220

acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca     2280

acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc     2340

tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact     2400

catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat     2460

ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttggt     2520

aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc     2580

accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat     2640

agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcctc     2700

acacaaaaaa ccaacacaca gatgtctagt agctctgatc ttttattcta gcggccgcgt     2760

accgaagcta tcattgccgc cttctatggc ggcatctcac gcagaagaag atgcccaggc     2820

cgatgaacag cagcagtccg gccactccgc cgagcactat cagcgccatg cggccgcctt     2880

ccagcttgag caattccacg tcgaacacga gggttgcgtg cggcggaatg attccgggat     2940

ggccggtagc gccgtaggca tagtcagggg agatcgtcag cttggcccgc tggccgacgc     3000

tcatctgggc gactccctct tcccagcccc ggatgacttc ctgctttcca agcataaact     3060

tgaaaggctt gttgcggtcc ctggagctgt caaacttctt tccatcttcg agcattccgg     3120

tatagtgcac cacacaggtc tgtccgcgct tcgggaaggt gcgcccgtcg ccgggggaaa     3180

tggtttccac ctggaccccc gaaccaccac cccccctagg ggaggagaca gtcacggaag     3240

ttccttgtcc ccagtagtcc atggcgtagg aaccgccgta gtagtagtgc ttggcacagt     3300

agtagatcgc ggtgtcgtca gtctgcaagc tattcatctt caggaacact tgggacttgg     3360

aattgtcctt gatgatggtg agccgagact tcagggcgct gttgtagtat gtggtttctg     3420

agccccaaat cacgccgagc cattccaggc ccttgcgagg tggctgccga atccatgaca     3480

ctccgtagtc gggcaaggac actcccgaca cggtgcaagt gacggagagc gactggctcg     3540

gggccaccag tcccgggccg gattcctgca gcttgacttc tcctttcgtc gatccctctc     3600

cgctgcccgg cttcccgctg cccgaggttg atccggtgat ctcgagcttg gttccaccac     3660

cgaaagtgta gggcagggtg tttccttgtt ggcagaagta ggtcgcaatg tcctcctgct     3720

ccaggttgga aatggtcagc gaataatcag tccctgaacc cgagccggaa aaccgggagg     3780

gcactccgga atgaaggcgg gaagtatggt agatcagcag tttcacggtc ccgtccggtt     3840

tttgctggta ccagttcagg tacttggaga tatcctgcga cgcccggcag gagatagtca     3900

cgcgatcacc cagagaggcg ctcagggatg aggtggtctg ggtcatctgg atgtcaccgg     3960

tggagcccgg cacccagagg agcaggaccc acagaagcag ggtatcagtc tccatggtgg     4020

cacgcgcctt gctagctaga caaaagtgtt gtggaattgc tccaggcgat ctgacggttc     4080

actaaacgag ctctgctttt ataggcgccc accgtacacg cctaagcgtc gacggaatga     4140

aagaccccac ctgtaggttt ggcaagctag gatcaaggtt aggaacagag agacagcaga     4200

atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac     4260

agttggaaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     4320

ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa     4380

ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact     4440

aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa     4500

gagcccacaa cccctcactc ggcgcgattc acctgacgcg taccatggcc ctccctgtga     4560

ccgccctgct gctccccctc gccctgttgc tccatgctgc ccgacctgga tccatccttt     4620

ggcacgagat gtggcacgag ggactcgaag aagcgtcccg gctgtacttc ggagagcgga     4680

acgtgaaggg gatgttcgaa gtgctggaac ccctgcacgc catgatggag cggggtcctc     4740

agacccttaa agaaacaagc ttcaaccagg cgtacgggcg cgacctgatg gaagcccagg     4800

agtggtgccg caagtacatg aagtccggaa acgtgaagga tctgctgcaa gcctgggatc     4860

tgtactacca cgtgttcaga aggatctcaa aggctagcgc cggcactggt tcggatatct     4920

acatttgggc accgctcgcc ggcacttgtg gagtgctgtt gctgtccctc gtgatcacca     4980

tgcataagag gggacggaag aagctgctgt acattttcaa gcagccattc atgcggcctg     5040

tgcaaaccac ccaggaggag gacgggtgca gctgccggtt ccctgaggaa gaggagggcg     5100

gatgcgaact gcgcgtgaag ttcagccgga gcgcagatgc tcccgcatac caacagggac     5160

agaaccagct gtataacgag ctgaacctgg gcagaaggga agagtacgac gtcctcgaca     5220

agcggcgggg acgcgaccca gaaatgggag gaaagccccg ccggaagaac ccgcaggaag     5280

gcctgtacaa cgagttgcag aaagacaaga tggctgaagc ttactcggag attggcatga     5340

agggggagag aagaagaggg aagggccacg acggccttta ccaaggactg agcactgcca     5400

ccaaggacac ctacgatgcg ctgcacatgc aggccctgcc cccgcggtga agcggccaac     5460

tcgacgttat tcccttcgaa ggaaacctgc aggtaccttt aagaccaatg acttacaagg     5520

cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact     5580

cccaaagaag acaagatctg ctttttgcct gtactgggtc tctctggtta gaccagatct     5640

gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc     5700

cttgagtgct tcaatgtgtg tgttggtttt ttgtgtgtcg aaattctagc gattctagct     5760

tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac     5820

acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac     5880

tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc     5940

tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg     6000

cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc     6060

actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt     6120

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     6180

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     6240

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     6300

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     6360

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     6420

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     6480

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     6540

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     6600

acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg     6660

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt     6720

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     6780

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga     6840

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa     6900

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac     6960

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga     7020

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc     7080

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca     7140

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta     7200

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg     7260

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc     7320

gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg     7380

ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt     7440

ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt     7500

cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata     7560

ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc     7620

gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac     7680

ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa     7740

ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct     7800

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat     7860

ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc     7920

cacctgggac tagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct     7980

ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc     8040

catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg     8100

gcgggactat ggttgctgac taattgagat gagcttgcat gccgacattg attattgact     8160

agtccctaag aaaccattct tatcatgaca ttaacctata aaaataggcg tatcacgagg     8220

ccctttcgtc                                                            8230


<210>  74
<211>  7135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_sEF1a-CD123target-T2A-Puro lentiviral vector

<400>  74
aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg       60

gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag      120

tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc      180

agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac acctgcaggg      240

ccgccaccat ggtcctcctt tggctcacgc tgctcctgat cgccctgccc tgtctcctgc      300

aaacgaagga agatccaaac ccaccaatca cgaacctaag gatgaaagca aaggctcagc      360

agttgacctg ggaccttaac agaaatgtga ccgatatcga gtgtgttaaa gacgccgact      420

attctatgcc ggcagtgaac aatagctatt gccagtttgg agcaatttcc ttatgtgaag      480

tgaccaacta caccgtccga gtggccaacc caccattctc cacgtggatc ctcttccctg      540

agaacagtgg gaagccttgg gcaggtgcgg agaatctgac ctgctggatt catgacgtgg      600

atttcttgag ctgcagctgg gcggtaggcc cgggggcccc cgcggacgtc cagtacgacc      660

tgtacttgaa cgttgccaac aggcgtcaac agtacgagtg tcttcactac aaaacggatg      720

ctcagggaac acgtatcggg tgtcgtttcg atgacatctc tcgactctcc agcggttctc      780

aaagttccca catcctggtg cggggcagga gcgcagcctt cggtatcccc tgcacagata      840

agtttgtcgt cttttcacag attgagatat taactccacc caacatgact gcaaagtgta      900

ataagacaca ttcctttatg cactggaaaa tgagaagtca tttcaatcgc aaatttcgct      960

atgagcttca gatacaaaag agaatgcagc ctgtaatcac agaacaggtc agagacagaa     1020

cctccttcca gctactcaat cctggaacgt acacagtaca aataagagcc cgggaaagag     1080

tgtatgaatt cttgagcgcc tggagcaccc cccagcgctt cgagtgcgac caggaggagg     1140

gcgcaaacac acgtgcctgg cggacgtcgc tgctgatcgc gctggggacg ctgctggccc     1200

tggtctgtgt cttcgtgatc tgcagaaggt atctggtgat gcagagactc tttccccgca     1260

tccctcacat gaaagacccc atcggtgaca gcttccaaaa cgacaagctg gtggtctggg     1320

aggcgggcaa agccggcctg gaggagtgtc tggtgactga agtacaggtc gtgcagaaaa     1380

ctggcggccg ctccggtgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga     1440

atccgggccc ctctagaacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg     1500

tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca     1560

ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc     1620

gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct     1680

ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg     1740

ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc     1800

accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg     1860

gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg     1920

tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct     1980

tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca     2040

agcccggtgc cctcgagtga tgagagtcat cgtcgaccga gcatcttacc gccatttata     2100

cccatatttg ttctgttttt cttgatttgg gtatacattt aaatgttaat agaacaaaat     2160

ggtggggcaa tcatttacat ttttagggat atgtaattac tagttcaggt gtattgccac     2220

aagacaaaca tgttaagaaa ctttcccgtt atttacgctc tgttcctgtt aatcaacctc     2280

tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct ccttttacgc     2340

tgtgtggata tgctgcttta tagcctctgt atctagctat tgcttcccgt acggctttcg     2400

ttttctcctc cttgtataaa tcctggttgc tgtctctttt agaggagttg tggcccgttg     2460

tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact ggctggggca     2520

ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg atcgccacgg     2580

cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg ctgggcactg     2640

ataattccgt ggtgttgtca tcgaattcgg taccttttta aaagaaaagg ggggactgga     2700

agggctaatt cactcccaac gaagacaaga tatcataact tcgtatagca tacattatac     2760

gaagttataa tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatatgttt     2820

atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttgctttttg cttgtactgg     2880

gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact     2940

gcttaagcct caataaagct tgcctcgacc agcctcgact gtgccttcta gttgccagcc     3000

atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt     3060

cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct     3120

ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc     3180

tggggatgcg gtgggctcta tggcctgcag ctgcattaat gaatcggcca acgcgcgggg     3240

agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg     3300

gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca     3360

gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac     3420

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac     3480

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg     3540

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     3600

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     3660

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     3720

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     3780

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     3840

gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt     3900

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     3960

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     4020

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     4080

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc     4140

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct     4200

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     4260

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     4320

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     4380

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     4440

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     4500

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     4560

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     4620

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     4680

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     4740

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     4800

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     4860

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     4920

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     4980

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     5040

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     5100

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     5160

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcaatgtagt cttatgcaat     5220

actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa     5280

aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag     5340

gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat     5400

tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc     5460

ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg     5520

agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag     5580

acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg     5640

aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca     5700

agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag     5760

gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa     5820

aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca     5880

agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt     5940

agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca     6000

ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc     6060

aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa     6120

gcggccctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta     6180

tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga     6240

agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg     6300

ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa     6360

ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag     6420

catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg     6480

gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt     6540

tgcaccactg ctgtgccttg gaatgctagt tggagtaatg aatctctgga acagatttgg     6600

aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac     6660

tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta     6720

gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa     6780

ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct     6840

atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc     6900

ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac     6960

agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg     7020

gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa     7080

actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatta cgcgt          7135


<210>  75
<211>  7735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCVL_MND-CD8ss-DmrC-CD3scFv-P2A-IgKss-CD19scFv-DmrA lentiviral 
       vector

<400>  75
taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca       60

taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt      120

ttatcgatta cgcgtgtaca gagagacagc agaatatggg ccaaacagga tatctgtggt      180

aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat atgggccaaa      240

caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca      300

gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa      360

ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg      420

ttcgcgcgct tctgctcccc gagctctata taagcagagc tcgtttagtg aaccgtcaga      480

tccctgcagg gccgccacca tggccctccc tgtgaccgcc ctgctgctcc ccctcgccct      540

gttgctccat gctgcccgac ctggatccat cctttggcac gagatgtggc acgagggact      600

cgaagaagcg tcccggctgt acttcggaga gcggaacgtg aaggggatgt tcgaagtgct      660

ggaacccctg cacgccatga tggagcgggg tcctcagacc cttaaagaaa caagcttcaa      720

ccaggcgtac gggcgcgacc tgatggaagc ccaggagtgg tgccgcaagt acatgaagtc      780

cggaaacgtg aaggatctgc tgcaagcctg ggatctgtac taccacgtgt tcagaaggat      840

ctcaaaggct agccaggtgc aactggtgca gagcggcggt ggcgttgtgc agccgggccg      900

cagcctgcgc ctgtcttgca aagcgagcgg ctataccttt acgcgctata ccatgcactg      960

ggtgcgccag gcgccgggca aaggtctgga atggattggc tatattaacc cgtctcgcgg     1020

ctataccaac tataatcaga aagtgaaaga tcgctttacc attagccgcg ataactctaa     1080

aaacaccgcg tttctgcaga tggatagcct gcgcccggaa gataccggcg tgtatttttg     1140

cgcgcgctac tatgatgacc attatagcct ggattattgg ggccagggca ccccggtgac     1200

cgttagctcg ggcagcacaa gcggcagcgg caagcctgga tctggcgagg gaagcaccaa     1260

gggcgatatc cagatgaccc agagcccgag ctctctgagc gcgagcgtgg gcgatcgcgt     1320

gaccattacg tgcagcgcgt ctagctctgt gagctatatg aactggtacc agcaaacccc     1380

aggcaaagcg ccgaaacgct ggatttatga taccagcaaa ctggcgagcg gcgtgccgag     1440

ccgctttagc ggctctggta gcggcaccga ttatacgttt accattagct ctctgcagcc     1500

ggaagatatt gcgacctatt actgccagca atggagctct aacccgttta cctttggcca     1560

gggtaccaaa ctgcagatta cccgctccgg ttcgggcgcg actaacttca gcctgctgaa     1620

gcaggccgga gatgtggagg aaaaccctgg accgtccatg gagactgata ccctgcttct     1680

gtgggtcctg ctcctctggg tgccgggctc caccggtgac atccagatga cccagaccac     1740

ctcatccctg agcgcctctc tgggtgatcg cgtgactatc tcctgccggg cgtcgcagga     1800

tatctccaag tacctgaact ggtaccagca aaaaccggac gggaccgtga aactgctgat     1860

ctaccatact tcccgccttc attccggagt gccctcccgg ttttccggct cgggttcagg     1920

gactgattat tcgctgacca tttccaacct ggagcaggag gacattgcga cctacttctg     1980

ccaacaagga aacaccctgc cctacacttt cggtggtgga accaagctcg agatcaccgg     2040

atcaacctcg ggcagcggga agccgggcag cggagaggga tcgacgaaag gagaagtcaa     2100

gctgcaggaa tccggcccgg gactggtggc cccgagccag tcgctctccg tcacttgcac     2160

cgtgtcggga gtgtccttgc ccgactacgg agtgtcatgg attcggcagc cacctcgcaa     2220

gggcctggaa tggctcggcg tgatttgggg ctcagaaacc acatactaca acagcgccct     2280

gaagtctcgg ctcaccatca tcaaggacaa ttccaagtcc caagtgttcc tgaagatgaa     2340

tagcttgcag actgacgaca ccgcgatcta ctactgtgcc aagcactact actacggcgg     2400

ttcctacgcc atggactact ggggacaagg aacttccgtg actgtctcct cccctagggg     2460

gggtggtggt tcgggggtcc aggtggaaac catttccccc ggcgacgggc gcaccttccc     2520

gaagcgcgga cagacctgtg tggtgcacta taccggaatg ctcgaagatg gaaagaagtt     2580

tgacagctcc agggaccgca acaagccttt caagtttatg cttggaaagc aggaagtcat     2640

ccggggctgg gaagagggag tcgcccagat gagcgtcggc cagcgggcca agctgacgat     2700

ctcccctgac tatgcctacg gcgctaccgg ccatcccgga atcattccgc cgcacgcaac     2760

cctcgtgttc gacgtggaat tgctcaagct ggaatgaggc ggccgcgtcg accgagcatc     2820

ttaccgccat ttatacccat atttgttctg tttttcttga tttgggtata catttaaatg     2880

ttaatagaac aaaatggtgg ggcaatcatt tacattttta gggatatgta attactagtt     2940

caggtgtatt gccacaagac aaacatgtta agaaactttc ccgttattta cgctctgttc     3000

ctgttaatca acctctggat tacaaaattt gtgaaagatt gactgatatt cttaactatg     3060

ttgctccttt tacgctgtgt ggatatgctg ctttatagcc tctgtatcta gctattgctt     3120

cccgtacggc tttcgttttc tcctccttgt ataaatcctg gttgctgtct cttttagagg     3180

agttgtggcc cgttgtccgt caacgtggcg tggtgtgctc tgtgtttgct gacgcaaccc     3240

ccactggctg gggcattgcc accacctgtc aactcctttc tgggactttc gctttccccc     3300

tcccgatcgc cacggcagaa ctcatcgccg cctgccttgc ccgctgctgg acaggggcta     3360

ggttgctggg cactgataat tccgtggtgt tgtcatcgaa ttcggtacct ttttaaaaga     3420

aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatca taacttcgta     3480

tagcatacat tatacgaagt tataatttat ttgtgaaatt tgtgatgcta ttgctttatt     3540

tgtaaccata tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccattgct     3600

ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta     3660

actagggaac ccactgctta agcctcaata aagcttgcct cgaccagcct cgactgtgcc     3720

ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg     3780

tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag     3840

gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga     3900

caatagcagg catgctgggg atgcggtggg ctctatggcc tgcagctgca ttaatgaatc     3960

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact     4020

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta     4080

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag     4140

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc     4200

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta     4260

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg     4320

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc     4380

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac     4440

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac     4500

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     4560

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     4620

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     4680

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     4740

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     4800

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg     4860

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat     4920

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc     4980

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg     5040

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct     5100

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca     5160

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg     5220

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg     5280

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc     5340

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag     5400

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg     5460

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag     5520

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat     5580

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg     5640

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca     5700

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca     5760

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat     5820

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag     5880

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat     5940

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc     6000

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg     6060

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc     6120

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt     6180

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca     6240

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa     6300

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac     6360

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct     6420

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact     6480

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt     6540

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa     6600

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa     6660

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca     6720

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata     6780

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag     6840

accaccgcac agcaagcggc cctgatcttc agacctggag gaggagatat gagggacaat     6900

tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     6960

accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     7020

ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     7080

gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     7140

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     7200

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     7260

tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taatgaatct     7320

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     7380

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     7440

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     7500

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     7560

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     7620

acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     7680

gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggt          7735


