                         SEQUENCE LISTING

<110>  Castelli, Maria
       Menzella, Hugo
       Peiru, Salvador
       Vetcher, Leandro
 
<120>  MODIFIED BACILLUS CEREUS PHOSPHOLIPASE C PROTEIN AND METHOD 
       OF PROCESSING VEGETABLE OIL

<130>  KCLN-001WO

<150>  61/842,880
<151>  2013-07-03

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  245
<212>  PRT
<213>  Bacillus cereus

<400>  1

Trp Ser Ala Glu Asp Lys His Lys Glu Gly Val Asn Ser His Leu Trp 
1               5                   10                  15      


Ile Val Asn Arg Ala Ile Asp Ile Met Ser Arg Asn Thr Thr Leu Val 
            20                  25                  30          


Lys Gln Asp Arg Val Ala Leu Leu Asn Glu Trp Arg Thr Glu Leu Glu 
        35                  40                  45              


Asn Gly Ile Tyr Ala Ala Asp Tyr Glu Asn Pro Tyr Tyr Asp Asn Ser 
    50                  55                  60                  


Thr Phe Ala Ser His Phe Tyr Asp Pro Asp Asn Gly Lys Thr Tyr Ile 
65                  70                  75                  80  


Pro Tyr Ala Lys Gln Ala Lys Glu Thr Gly Ala Lys Tyr Phe Lys Leu 
                85                  90                  95      


Ala Gly Glu Ser Tyr Lys Asn Lys Asp Met Lys Gln Ala Phe Phe Tyr 
            100                 105                 110         


Leu Gly Leu Ser Leu His Tyr Leu Gly Asp Val Asn Gln Pro Met His 
        115                 120                 125             


Ala Ala Asn Phe Thr Asn Leu Ser Tyr Pro Gln Gly Phe His Ser Lys 
    130                 135                 140                 


Tyr Glu Asn Phe Val Asp Thr Ile Lys Asp Asn Tyr Lys Val Thr Asp 
145                 150                 155                 160 


Gly Asn Gly Tyr Trp Asn Trp Lys Gly Thr Asn Pro Glu Asp Trp Ile 
                165                 170                 175     


His Gly Ala Ala Val Val Ala Lys Gln Asp Tyr Ala Gly Ile Val Asn 
            180                 185                 190         


Asp Asn Thr Lys Asp Trp Phe Val Arg Ala Ala Val Ser Gln Glu Tyr 
        195                 200                 205             


Ala Asp Lys Trp Arg Ala Glu Val Thr Pro Met Thr Gly Lys Arg Leu 
    210                 215                 220                 


Met Asp Ala Gln Arg Val Thr Ala Gly Tyr Ile Gln Leu Trp Phe Asp 
225                 230                 235                 240 


Thr Tyr Gly Asn Arg 
                245 


<210>  2
<211>  738
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  2
tggtccgcag aagacaaaca taaggaaggt gtgaatagcc atttgtggat tgtgaaccgt       60

gccattgaca tcatgtcccg taataccacc ctggttaaac aagatcgcgt ggccctgtta      120

aatgaatggc gtactgaatt agaaaacggt atctatgctg cagattacga aaatccatat      180

tacgatgact ctacctgggc gtcacatttc tatgatccag acaacggcaa aacatatatc      240

ccgtacgcta aacaagcaaa ggaaacgggt gctaaatatt ttaagctggc aggcgaatcc      300

tacaagaata aggatatgaa gcaagcattt ttctatctgg gtctgtcgtt acattactta      360

ggcgatgtca accaacctat gcacgccgcg agcttcactg acttgagtta tccacagggt      420

ttccattcaa agtacgaaaa cttcgtcgat acaattaaag acaactacaa agtaacggat      480

ggtaatggct actggaactg gaagggtaca aatccggaag attggattca cggcgctgca      540

gttgtggcca aacaagacta tgcgggtatc gttaatgata acacgaagga ctggtttgtc      600

agagccgctg tttctcagga atacgctgat aaatggcgcg cagaagttac ccctatgact      660

ggcaagcgtt tgatggatgc ccaaagagtg accgcgggtt atatccagct gtggtttgac      720

acttacggca accgttaa                                                    738


<210>  3
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered PLC

<400>  3

Trp Ser Ala Glu Asp Lys His Lys Glu Gly Val Asn Ser His Leu Trp 
1               5                   10                  15      


Ile Val Asn Arg Ala Ile Asp Ile Met Ser Arg Asn Thr Thr Leu Val 
            20                  25                  30          


Lys Gln Asp Arg Val Ala Leu Leu Asn Glu Trp Arg Thr Glu Leu Glu 
        35                  40                  45              


Asn Gly Ile Tyr Ala Ala Asp Tyr Glu Asn Pro Tyr Tyr Asp Asp Ser 
    50                  55                  60                  


Thr Trp Ala Ser His Phe Tyr Asp Pro Asp Asn Gly Lys Thr Tyr Ile 
65                  70                  75                  80  


Pro Tyr Ala Lys Gln Ala Lys Glu Thr Gly Ala Lys Tyr Phe Lys Leu 
                85                  90                  95      


Ala Gly Glu Ser Tyr Lys Asn Lys Asp Met Lys Gln Ala Phe Phe Tyr 
            100                 105                 110         


Leu Gly Leu Ser Leu His Tyr Leu Gly Asp Val Asn Gln Pro Met His 
        115                 120                 125             


Ala Ala Ser Phe Thr Asp Leu Ser Tyr Pro Gln Gly Phe His Ser Lys 
    130                 135                 140                 


Tyr Glu Asn Phe Val Asp Thr Ile Lys Asp Asn Tyr Lys Val Thr Asp 
145                 150                 155                 160 


Gly Asn Gly Tyr Trp Asn Trp Lys Gly Thr Asn Pro Glu Asp Trp Ile 
                165                 170                 175     


His Gly Ala Ala Val Val Ala Lys Gln Asp Tyr Ala Gly Ile Val Asn 
            180                 185                 190         


Asp Asn Thr Lys Asp Trp Phe Val Arg Ala Ala Val Ser Gln Glu Tyr 
        195                 200                 205             


Ala Asp Lys Trp Arg Ala Glu Val Thr Pro Met Thr Gly Lys Arg Leu 
    210                 215                 220                 


Met Asp Ala Gln Arg Val Thr Ala Gly Tyr Ile Gln Leu Trp Phe Asp 
225                 230                 235                 240 


Thr Tyr Gly Asn Arg 
                245 


<210>  4
<211>  738
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<400>  4
tggtccgcag aagacaaaca taaggaaggt gtgaatagcc atttgtggat tgtgaaccgt       60

gccattgaca tcatgtcccg taataccacc ctggttaaac aagatcgcgt ggccctgtta      120

aatgaatggc gtactgaatt agaaaacggt atctatgctg cagattacga aaatccatat      180

tacgatgact ctacctacgc gtcacatttc tatgatccag acaacggcaa aacatatatc      240

ccgtacgcta aacaagcaaa ggaaacgggt gctaaatatt ttaagctggc aggcgaatcc      300

tacaagaata aggatatgaa gcaagcattt ttctatctgg gtctgtcgtt acattactta      360

ggcgatgtca accaacctat gcacgccgcg agcttcactg acttgagtta tccacagggt      420

ttccattcaa agtacgaaaa cttcgtcgat acaattaaag acaactacaa agtaacggat      480

ggtaatggct actggaactg gaagggtaca aatccggaag attggattca cggcgctgca      540

gttgtggcca aacaagacta tgcgggtatc gttaatgata acacgaagga ctggtttgtc      600

agagccgctg tttctcagga atacgctgat aaatggcgcg cagaagttac ccctatgact      660

ggcaagcgtt tgatggatgc ccaaagagtg accgcgggtt atatccagct gtggtttgac      720

acttacggca accgttaa                                                    738


<210>  5
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered PLC

<400>  5

Trp Ser Ala Glu Asp Lys His Lys Glu Gly Val Asn Ser His Leu Trp 
1               5                   10                  15      


Ile Val Asn Arg Ala Ile Asp Ile Met Ser Arg Asn Thr Thr Leu Val 
            20                  25                  30          


Lys Gln Asp Arg Val Ala Leu Leu Asn Glu Trp Arg Thr Glu Leu Glu 
        35                  40                  45              


Asn Gly Ile Tyr Ala Ala Asp Tyr Glu Asn Pro Tyr Tyr Asp Asp Ser 
    50                  55                  60                  


Thr Tyr Ala Ser His Phe Tyr Asp Pro Asp Asn Gly Lys Thr Tyr Ile 
65                  70                  75                  80  


Pro Tyr Ala Lys Gln Ala Lys Glu Thr Gly Ala Lys Tyr Phe Lys Leu 
                85                  90                  95      


Ala Gly Glu Ser Tyr Lys Asn Lys Asp Met Lys Gln Ala Phe Phe Tyr 
            100                 105                 110         


Leu Gly Leu Ser Leu His Tyr Leu Gly Asp Val Asn Gln Pro Met His 
        115                 120                 125             


Ala Ala Ser Phe Thr Asp Leu Ser Tyr Pro Gln Gly Phe His Ser Lys 
    130                 135                 140                 


Tyr Glu Asn Phe Val Asp Thr Ile Lys Asp Asn Tyr Lys Val Thr Asp 
145                 150                 155                 160 


Gly Asn Gly Tyr Trp Asn Trp Lys Gly Thr Asn Pro Glu Asp Trp Ile 
                165                 170                 175     


His Gly Ala Ala Val Val Ala Lys Gln Asp Tyr Ala Gly Ile Val Asn 
            180                 185                 190         


Asp Asn Thr Lys Asp Trp Phe Val Arg Ala Ala Val Ser Gln Glu Tyr 
        195                 200                 205             


Ala Asp Lys Trp Arg Ala Glu Val Thr Pro Met Thr Gly Lys Arg Leu 
    210                 215                 220                 


Met Asp Ala Gln Arg Val Thr Ala Gly Tyr Ile Gln Leu Trp Phe Asp 
225                 230                 235                 240 


Thr Tyr Gly Asn Arg 
                245 


<210>  6
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered PLC

<400>  6

Trp Ser Ala Glu Asp Lys His Lys Glu Gly Val Asn Ser His Leu Trp 
1               5                   10                  15      


Ile Val Asn Arg Ala Ile Asp Ile Met Ser Arg Asn Thr Thr Leu Val 
            20                  25                  30          


Lys Gln Asp Arg Val Ala Leu Leu Asn Glu Trp Arg Thr Glu Leu Glu 
        35                  40                  45              


Asn Gly Ile Tyr Ala Ala Asp Tyr Glu Asn Pro Tyr Tyr Asp Asp Ser 
    50                  55                  60                  


Thr Phe Ala Ser His Phe Tyr Asp Pro Asp Asn Gly Lys Thr Tyr Ile 
65                  70                  75                  80  


Pro Tyr Ala Lys Gln Ala Lys Glu Thr Gly Ala Lys Tyr Phe Lys Leu 
                85                  90                  95      


Ala Gly Glu Ser Tyr Lys Asn Lys Asp Met Lys Gln Ala Phe Phe Tyr 
            100                 105                 110         


Leu Gly Leu Ser Leu His Tyr Leu Gly Asp Val Asn Gln Pro Met His 
        115                 120                 125             


Ala Ala Ser Phe Thr Asp Leu Ser Tyr Pro Gln Gly Phe His Ser Lys 
    130                 135                 140                 


Tyr Glu Asn Phe Val Asp Thr Ile Lys Asp Asn Tyr Lys Val Thr Asp 
145                 150                 155                 160 


Gly Asn Gly Tyr Trp Asn Trp Lys Gly Thr Asn Pro Glu Asp Trp Ile 
                165                 170                 175     


His Gly Ala Ala Val Val Ala Lys Gln Asp Tyr Ala Gly Ile Val Asn 
            180                 185                 190         


Asp Asn Thr Lys Asp Trp Phe Val Arg Ala Ala Val Ser Gln Glu Tyr 
        195                 200                 205             


Ala Asp Lys Trp Arg Ala Glu Val Thr Pro Met Thr Gly Lys Arg Leu 
    210                 215                 220                 


Met Asp Ala Gln Arg Val Thr Ala Gly Tyr Ile Gln Leu Trp Phe Asp 
225                 230                 235                 240 


Thr Tyr Gly Asn Arg 
                245 


<210>  7
<211>  20
<212>  PRT
<213>  Bacillus cereus

<400>  7

Glu Asn Pro Tyr Tyr Asp Asn Ser Thr Phe Ala Ser His Phe Tyr Asp 
1               5                   10                  15      


Pro Asp Asn Gly 
            20  


