                         SEQUENCE LISTING

<110>  DANA-FARBER CANCER INSTITUTE, INC.
 
<120>  Anti-Galectin-1 (Gal1) Monoclonal Antibodies And Fragments 
       Thereof For Neutralizing Gal1

<130>  DFS-222.25

<140>  PCT/US2014/047784
<141>  2014-07-23

<150>  61/911,031
<151>  2013-12-03

<150>  61/857,839
<151>  2013-07-24

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  408
<212>  DNA
<213>  Homo sapiens

<400>  1
atggcttgtg gtctggtcgc cagcaacctg aatctcaaac ctggagagtg ccttcgagtg       60

cgaggcgagg tggctcctga cgctaagagc ttcgtgctga acctgggcaa agacagcaac      120

aacctgtgcc tgcacttcaa ccctcgcttc aacgcccacg gcgacgccaa caccatcgtg      180

tgcaacagca aggacggcgg ggcctggggg accgagcagc gggaggctgt ctttcccttc      240

cagcctggaa gtgttgcaga ggtgtgcatc accttcgacc aggccaacct gaccgtcaag      300

ctgccagatg gatacgaatt caagttcccc aaccgcctca acctggaggc catcaactac      360

atggcagctg acggtgactt caagatcaaa tgtgtggcct ttgactga                   408


<210>  2
<211>  135
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Arg Val Arg Gly Glu Val Ala Pro Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Gly Gly Ala Trp Gly Thr Glu Gln Arg Glu Ala Val Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Val Ala Glu Val Cys Ile Thr Phe Asp Gln Ala Asn 
                85                  90                  95      


Leu Thr Val Lys Leu Pro Asp Gly Tyr Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Asp 
    130                 135 


<210>  3
<211>  408
<212>  DNA
<213>  Mus musculus

<400>  3
atggcctgtg gtctggtcgc cagcaacctg aatctcaaac ctggggaatg tctcaaagtt       60

cggggagagg tggcctcgga cgccaagagc tttgtgctga acctgggaaa agacagcaac      120

aacctgtgcc tacacttcaa tcctcgcttc aatgcccatg gagacgccaa caccattgtg      180

tgtaacacca aggaagatgg gacctgggga accgaacacc gggaacctgc cttccccttc      240

cagcccggga gcatcacaga ggtgtgcatc acctttgacc aggctgacct gaccatcaag      300

ctgccagacg gacatgaatt caagttcccc aaccgcctca acatggaggc catcaactac      360

atggcggcgg atggagactt caagattaag tgcgtggcct ttgagtga                   408


<210>  4
<211>  135
<212>  PRT
<213>  Mus musculus

<400>  4

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Val Ala Ser Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Thr Lys 
    50                  55                  60                  


Glu Asp Gly Thr Trp Gly Thr Glu His Arg Glu Pro Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


<210>  5
<211>  408
<212>  DNA
<213>  Macaca mulatta

<400>  5
atggcttgtg gtctggtcgc cagcaacctg aatctcaaac ctggagagtg cctccgagtg       60

cggggcgagg tggcccccga cgccaagagc ttcgtgctga acctgggcaa agatagcaac      120

aacctgtgcc tgcacttcaa ccctcgcttc aacgcccacg gcgacgccaa caccatcgtg      180

tgcaacagca aggacggtgg ggcctggggg accgagcagc gggaggctgc ctttcctttc      240

cagcctggaa gtgtcgcaga ggtgtgcatc acctttgacc aggccgacct gaccatcaag      300

ctgccagatg gatacgaatt caagttcccc aaccgcctca acctggaggc catcaactac      360

atggcagctg acggtgactt caagatcaag tgtgtggcct ttgactga                   408


<210>  6
<211>  135
<212>  PRT
<213>  Macaca mulatta

<400>  6

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Arg Val Arg Gly Glu Val Ala Pro Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Gly Gly Ala Trp Gly Thr Glu Gln Arg Glu Ala Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Val Ala Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly Tyr Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Asp 
    130                 135 


<210>  7
<211>  408
<212>  DNA
<213>  Pan troglodytes

<400>  7
atggcttgtg gtctggtcgc cagcaacctg aatctcaaac ctggagagtg ccttcgagtg       60

cgaggcgagg tggcccctga cgctaagagc ttcgtgctga acctgggcaa agacagcaac      120

aacctgtgcc tgcacttcaa ccctcgcttc aacgcccacg gcgacgccaa caccatcgtg      180

tgcaacagca aggacggcgg ggcctggggg accgagcagc gggaggctgt ctttcccttc      240

cagcctggaa gtgttgcaga ggtgtgcatc accttcgacc aggccaacct gaccgtcaag      300

ctgccagatg gatacgaatt caagttcccc aaccgcctca acctggaggc catcaactac      360

atggcagctg acggtgactt caagatcaag tgtgtggcct ttgactga                   408


<210>  8
<211>  135
<212>  PRT
<213>  Pan troglodytes

<400>  8

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Arg Val Arg Gly Glu Val Ala Pro Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Gly Gly Ala Trp Gly Thr Glu Gln Arg Glu Ala Val Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Val Ala Glu Val Cys Ile Thr Phe Asp Gln Ala Asn 
                85                  90                  95      


Leu Thr Val Lys Leu Pro Asp Gly Tyr Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Asp 
    130                 135 


<210>  9
<211>  408
<212>  DNA
<213>  Rattus norvegicus

<400>  9
atggcctgtg gtctggtcgc cagcaacctg aatctcaaac ctggggaatg tctcaaagtt       60

cggggagagc tggccccgga cgccaagagc tttgtgttga acctggggaa agacagcaac      120

aacctgtgcc tacacttcaa cccccgcttc aacgcccacg gagatgccaa caccattgtg      180

tgtaacagca aggacgatgg gacctgggga acagaacaac gggagactgc cttccctttc      240

cagcctggga gcatcacgga ggtgtgcatc acctttgacc aggctgacct gaccatcaag      300

ctgccagacg ggcatgaatt caaattcccc aaccgcctca acatggaggc catcaactac      360

atggcggcgg atggtgactt caagattaag tgtgtggcct ttgagtga                   408


<210>  10
<211>  135
<212>  PRT
<213>  Rattus norvegicus

<400>  10

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Leu Ala Pro Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Asp Gly Thr Trp Gly Thr Glu Gln Arg Glu Thr Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


<210>  11
<211>  408
<212>  DNA
<213>  Canis familiaris

<400>  11
atggcttgtg gtctggtcgc cagcaatctg agtctcaaac ctgggcagtg cctcagagtg       60

caatgcgagg tggtccccga agccaagagc ttcgtgctga acctgggcaa agacggggac      120

aacctgtgcc tgcacttcaa ccctcgcttt gaagcccatg gcgacgtcaa caccattgtg      180

tgtaacagca aggatggcgg ggcctggggc gaggagcttc gagagtccgc cttccccttc      240

cagcccggga ctgtcacaga ggtgtgcatc tccttcgacc aggctgactt gaccatcaag      300

ctgccagatg gatacacctt caagttcccc aaccgcctca acctggaggc catcagctac      360

ctggcagctg atggtgacat gaagatcaag tgcctggcct ttgactaa                   408


<210>  12
<211>  135
<212>  PRT
<213>  Canis familiaris

<400>  12

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Ser Leu Lys Pro Gly Gln 
1               5                   10                  15      


Cys Leu Arg Val Gln Cys Glu Val Val Pro Glu Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Gly Asp Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Glu Ala His Gly Asp Val Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Gly Gly Ala Trp Gly Glu Glu Leu Arg Glu Ser Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Thr Val Thr Glu Val Cys Ile Ser Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly Tyr Thr Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Ser Tyr Leu Ala Ala Asp Gly Asp Met Lys 
        115                 120                 125             


Ile Lys Cys Leu Ala Phe Asp 
    130                 135 


<210>  13
<211>  405
<212>  DNA
<213>  Gallus gallus

<400>  13
atggagcaag gactggttgt tacccagctg gatgtacagc ctggagagtg tgtcaaggtc       60

aaagggaaga tcctatccga tgccaaaggg ttttctgtga atgtagggaa ggacagcagc      120

acactcatgc ttcatttcaa ccctcgcttt gactgccatg gggatgtcaa cactgttgtg      180

tgcaactcaa aggaggatgg cacgtggggt gaggaggaca ggaaggctga cttccctttc      240

cagcagggcg acaaggttga gatctgtatc tcctttgatg cagcagaggt caaggtgaag      300

gtgcctgaag tggagtttga gtttcccaat cggctgggca tggagaaaat tcaatacctg      360

gctgtggagg gtgactttaa agtgaaagct attaagttca gctaa                      405


<210>  14
<211>  134
<212>  PRT
<213>  Gallus gallus

<400>  14

Met Glu Gln Gly Leu Val Val Thr Gln Leu Asp Val Gln Pro Gly Glu 
1               5                   10                  15      


Cys Val Lys Val Lys Gly Lys Ile Leu Ser Asp Ala Lys Gly Phe Ser 
            20                  25                  30          


Val Asn Val Gly Lys Asp Ser Ser Thr Leu Met Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asp Cys His Gly Asp Val Asn Thr Val Val Cys Asn Ser Lys 
    50                  55                  60                  


Glu Asp Gly Thr Trp Gly Glu Glu Asp Arg Lys Ala Asp Phe Pro Phe 
65                  70                  75                  80  


Gln Gln Gly Asp Lys Val Glu Ile Cys Ile Ser Phe Asp Ala Ala Glu 
                85                  90                  95      


Val Lys Val Lys Val Pro Glu Val Glu Phe Glu Phe Pro Asn Arg Leu 
            100                 105                 110         


Gly Met Glu Lys Ile Gln Tyr Leu Ala Val Glu Gly Asp Phe Lys Val 
        115                 120                 125             


Lys Ala Ile Lys Phe Ser 
    130                 


<210>  15
<211>  408
<212>  DNA
<213>  Bos taurus

<400>  15
atggcttgtg gtctggtcgc cagcaacctg aatctcaaac ctggggagtg cctcagagtg       60

cggggcgagg tggccgcaga cgccaagagc ttcttgctga acctgggcaa agacgacaac      120

aacctgtgcc tccacttcaa ccctcgtttc aacgcgcatg gggacgtcaa caccatcgtg      180

tgtaacagca aggacgctgg ggcctggggg gccgagcaga gggaatctgc cttccccttc      240

cagcctggaa gtgtcgtgga ggtatgcatc tccttcaacc agacggacct aaccatcaag      300

ctgcctgatg gatacgaatt caagttcccc aaccgcctca acctggaggc catcaactac      360

ctgtctgcag gtggtgactt caagatcaag tgtgtggcct ttgagtga                   408


<210>  16
<211>  135
<212>  PRT
<213>  Bos taurus

<400>  16

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Arg Val Arg Gly Glu Val Ala Ala Asp Ala Lys Ser Phe Leu 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Asp Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Val Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Ala Gly Ala Trp Gly Ala Glu Gln Arg Glu Ser Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Val Val Glu Val Cys Ile Ser Phe Asn Gln Thr Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly Tyr Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Asn Tyr Leu Ser Ala Gly Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


<210>  17
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8B5, 8F4, and 8G3 vH 
       cDNA sequence"

<400>  17
gaggttcagc tgcagcagtc tgtggcagag tttgtgaggc caggggcctc agtcaggttg       60

tcctgcacag cttctggctt caacattaaa aacacctata tacactgggt gaggcagagg      120

cctgaacagg gcctggagtg gattggaaag attgatcctg cgaatggtaa tactaaatat      180

gtcccggagt tccagggcaa ggccactatg actgcggaca catcctccaa cacagtctac      240

ctgcacctca gcagcctgac atctgaggac actgccatct attactgtgt cgatggttac      300

tacggctggt atttcgctgt ctggggcaca gggaccacgg tcaccgtctc ctca            354


<210>  18
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8B5, 8F4, and 8G3 vK 
       cDNA sequence"

<400>  18
caggctgttg tgactcagga atctgcactc accacatcac ctggtgaaac agtcacactc       60

acttgtcgct caagcactgg ggctgttaca actagtaact atgccaactg ggtccaagaa      120

aaaccagatc atttattcac tggtctaata ggtgctacca acaaccgagc tccaggtgtt      180

cctgccagat tctcaggctc cctgattgga gacaaggctg tcctcaccat cacaggggca      240

caaactgagg atgaggcaat atatttctgt gctctatggt acagaaacca ttttattttc      300

ggcagtggaa ccaaggtcac tgtcctc                                          327


<210>  19
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8B5, 8F4, and 8G3 vH 
       amino acid sequence"

<400>  19

Glu Val Gln Leu Gln Gln Ser Val Ala Glu Phe Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Arg Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asn Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Lys Ile Asp Pro Ala Asn Gly Asn Thr Lys Tyr Val Pro Glu Phe 
    50                  55                  60                  


Gln Gly Lys Ala Thr Met Thr Ala Asp Thr Ser Ser Asn Thr Val Tyr 
65                  70                  75                  80  


Leu His Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Val Asp Gly Tyr Tyr Gly Trp Tyr Phe Ala Val Trp Gly Thr Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser 
        115             


<210>  20
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8B5, 8F4, and 8G3 vK 
       amino acid sequence"

<400>  20

Gln Ala Val Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
        35                  40                  45              


Leu Ile Gly Ala Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Ile Gly Asp Lys Ala Val Leu Thr Ile Thr Gly Ala 
65                  70                  75                  80  


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Tyr Arg Asn 
                85                  90                  95      


His Phe Ile Phe Gly Ser Gly Thr Lys Val Thr Val Leu 
            100                 105                 


