                         SEQUENCE LISTING

<110>  DANA-FARBER CANCER INSTITUTE, INC.
 
<120>  Anti-Galectin-1 Monoclonal Antibodies And Fragments Thereof

<130>  DFS-122.25

<140>  PCT/US2014/047783
<141>  2014-07-23

<150>  61/911,031
<151>  2013-12-03

<150>  61/857,839
<151>  2013-07-24

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  408
<212>  DNA
<213>  Homo sapiens

<400>  1
atggcttgtg gtctggtcgc cagcaacctg aatctcaaac ctggagagtg ccttcgagtg       60

cgaggcgagg tggctcctga cgctaagagc ttcgtgctga acctgggcaa agacagcaac      120

aacctgtgcc tgcacttcaa ccctcgcttc aacgcccacg gcgacgccaa caccatcgtg      180

tgcaacagca aggacggcgg ggcctggggg accgagcagc gggaggctgt ctttcccttc      240

cagcctggaa gtgttgcaga ggtgtgcatc accttcgacc aggccaacct gaccgtcaag      300

ctgccagatg gatacgaatt caagttcccc aaccgcctca acctggaggc catcaactac      360

atggcagctg acggtgactt caagatcaaa tgtgtggcct ttgactga                   408


<210>  2
<211>  135
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Arg Val Arg Gly Glu Val Ala Pro Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Gly Gly Ala Trp Gly Thr Glu Gln Arg Glu Ala Val Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Val Ala Glu Val Cys Ile Thr Phe Asp Gln Ala Asn 
                85                  90                  95      


Leu Thr Val Lys Leu Pro Asp Gly Tyr Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Asp 
    130                 135 


<210>  3
<211>  408
<212>  DNA
<213>  Mus musculus

<400>  3
atggcctgtg gtctggtcgc cagcaacctg aatctcaaac ctggggaatg tctcaaagtt       60

cggggagagg tggcctcgga cgccaagagc tttgtgctga acctgggaaa agacagcaac      120

aacctgtgcc tacacttcaa tcctcgcttc aatgcccatg gagacgccaa caccattgtg      180

tgtaacacca aggaagatgg gacctgggga accgaacacc gggaacctgc cttccccttc      240

cagcccggga gcatcacaga ggtgtgcatc acctttgacc aggctgacct gaccatcaag      300

ctgccagacg gacatgaatt caagttcccc aaccgcctca acatggaggc catcaactac      360

atggcggcgg atggagactt caagattaag tgcgtggcct ttgagtga                   408


<210>  4
<211>  135
<212>  PRT
<213>  Mus musculus

<400>  4

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Val Ala Ser Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Thr Lys 
    50                  55                  60                  


Glu Asp Gly Thr Trp Gly Thr Glu His Arg Glu Pro Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


<210>  5
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK amino acid 
       sequence"

<400>  5

Met Met Ser Pro Ala Gln Phe Leu Phe Leu Leu Val Leu Trp Ile Gln 
1               5                   10                  15      


Lys Thr Asn Gly Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser 
            20                  25                  30          


Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
        35                  40                  45              


Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg 
    50                  55                  60                  


Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Leu Ser Lys Leu Asp 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Gln Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Phe Tyr Tyr 
            100                 105                 110         


Cys Trp Gln Gly Thr His Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys 
    130         


<210>  6
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK cDNA sequence"

<400>  6
atgatgagtc ctgcccagtt cctgtttctg ttagtgctct ggattcagaa aaccaacggt       60

gatgttgtga tgactcagac cccactcact ttgtcggtta ccattggaca accagcctcc      120

atctcttgca agtcaagtca gagcctctta gatagtgatg gaaagacata tttgaattgg      180

ttgttacaga ggccaggcca gtctccaaag cgcctaatct atctgctgtc taaactggac      240

tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgcaaatc      300

tacacgttcg gaggggggac caagctggaa ataaaa                                336


<210>  7
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH amino acid 
       sequence"

<400>  7

Met Gly Trp Ser Gly Ile Phe Leu Phe Leu Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Arg Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Arg Tyr Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Arg Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Phe Cys Thr Val Trp Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
        115                 120                 125             


Val Ser Ser 
    130     


<210>  8
<211>  393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH cDNA sequence"

<400>  8
atgggatgga gcgggatctt tctcttcctc ctgtcagtaa ctacaggtgt ccactcccag       60

gcttatctac agcagtctgg ggctgagctg gtgaggcctg gggcctcagt gaggatgtcc      120

tgcaaggctt ctggctacac tttcaccagg tacaatatgc actgggtaaa gcagacacct      180

agacagggcc tggaatggat tggacgtatt tatccaggaa atggtgatac ttcctacaat      240

cagaagttca agggcaaggc cacactgact gtagacaaat cctccagcac agcctacatg      300

cagctcagca gcctgacatc tgaagactct gcggtctatt tctgtacagt ctgggactac      360

tggggccaag gcaccactct cacagtctcc tca                                   393


<210>  9
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK signal peptide
       cDNA sequence"

<400>  9
atgatgagtc ctgcccagtt cctgtttctg ttagtgctct ggattcagaa aaccaacggt       60


<210>  10
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK framework 1 
       cDNA sequence"

<400>  10
gatgttgtga tgactcagac cccactcact ttgtcggtta ccattggaca accagcctcc       60

atctcttgc                                                               69


<210>  11
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK CDR-L1 cDNA 
       sequence"

<400>  11
aagtcaagtc agagcctctt agatagtgat ggaaagacat atttgaat                    48


<210>  12
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK CDR-L1 amino 
       acid sequence"

<400>  12

Lys Ser Ser Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  13
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK framework 2 
       cDNA sequence"

<400>  13
tggttgttac agaggccagg ccagtctcca aagcgcctaa tctat                       45


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK CDR-L2 cDNA 
       sequence"

<400>  14
ctgctgtcta aactggactc t                                                 21


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK CDR-L2 amino 
       acid sequence"

<400>  15

Leu Leu Ser Lys Leu Asp Ser 
1               5           


<210>  16
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK framework 3 
       cDNA sequence"

<400>  16
ggagtccctg acaggttcac tggcagtgga tcagggacag atttcacact gcaaatcagc       60

agagtggagg ctgaggattt gggattttat tattgc                                 96


<210>  17
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK CDR-L3 cDNA 
       sequence"

<400>  17
tggcaaggta cacattttcc ttacacg                                           27


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK CDR-L3 amino 
       acid sequence"

<400>  18

Trp Gln Gly Thr His Phe Pro Tyr Thr 
1               5                   


<210>  19
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vK J segment cDNA
       sequence"

<400>  19
ttcggagggg ggaccaagct ggaaataaaa                                        30


<210>  20
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH signal peptide
       cDNA sequence"

<400>  20
atgggatgga gcgggatctt tctcttcctc ctgtcagtaa ctacaggtgt ccactcc          57


<210>  21
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH framework 1 
       cDNA sequence"

<400>  21
caggcttatc tacagcagtc tggggctgag ctggtgaggc ctggggcctc agtgaggatg       60

tcctgcaagg cttctggcta cactttcacc                                        90


<210>  22
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH CDR-H1 cDNA 
       sequence"

<400>  22
aggtacaata tgcac                                                        15


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH CDR-H1 amino 
       acid sequence"

<400>  23

Arg Tyr Asn Met His 
1               5   


<210>  24
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH framework 2 
       cDNA sequence"

<400>  24
tgggtaaagc agacacctag acagggcctg gaatggattg ga                          42


<210>  25
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH CDR-H2 cDNA 
       sequence"

<400>  25
cgtatttatc caggaaatgg tgatacttcc tacaatcaga agttcaaggg c                51


<210>  26
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH CDR-H2 amino 
       acid sequence"

<400>  26

Arg Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  27
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH framework 3 
       cDNA sequence"

<400>  27
aaggccacac tgactgtaga caaatcctcc agcacagcct acatgcagct cagcagcctg       60

acatctgaag actctgcggt ctatttctgt acagtc                                 96


<210>  28
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: 8A12 vH J segment cDNA
       sequence"

<400>  28
tggggccaag gcaccactct cacagtctcc tca                                    33


