                         SEQUENCE LISTING

<110>  Myriant Corporation
       Yocum, R. Rogers
       Collard, Andrew
       Hermann, Theron
       Yu, Xiaohui
       Gong, Wei
 
<120>  Method of producing succinic acid and other chemicals using 
       facilitated diffusion for sugar import

<130>  MC2014-01PCT

<150>  US 61/857300
<151>  2013-07-23

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  9430
<212>  DNA
<213>  Escherichia coli


<220>
<221>  pAC19
<222>  (1)..(9430)
<223>  Plasmid used to install a glf-glk casette at the tdc locus of 
       bacterial strain KJ122

<400>  1
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc       60

agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag      120

gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc      180

tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa      240

aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg      300

aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca      360

gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt      420

gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta      480

gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc      540

tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta      600

cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac      660

ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata      720

gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa      780

atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct      840

gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt      900

tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat      960

tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta     1020

aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat     1080

tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata     1140

atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat     1200

taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat     1260

ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg     1320

ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata     1380

agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc     1440

tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca     1500

ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg     1560

caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg     1620

atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac     1680

tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca     1740

agactaacaa acaaaagtag aacaactgtt caccgttaca tatcaaaggg aaaactgtcc     1800

atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt     1860

ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta     1920

acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac     1980

ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg     2040

cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa     2100

tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga     2160

ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg     2220

cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca     2280

attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc     2340

cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc     2400

ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt     2460

gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct     2520

cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc     2580

gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct     2640

tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc     2700

gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt     2760

gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc     2820

gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac     2880

gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg     2940

tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga     3000

tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca     3060

tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca     3120

tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg     3180

gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg     3240

aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga     3300

gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc     3360

gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag     3420

gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag     3480

aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac     3540

aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg     3600

ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg     3660

gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt     3720

atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg     3780

cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg     3840

tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta     3900

actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc     3960

tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg     4020

aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta     4080

aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat     4140

agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt     4200

ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg     4260

tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat     4320

taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc     4380

ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg     4440

caggtcgact ctagaggatc ccccccgccg ccgacagagt aataggtttt acttaatagc     4500

tcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg     4560

ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact     4620

ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca     4680

tcctgcggac tgtttcctgc cagtcccatg atgatggcga catccgtggc atggcctttg     4740

cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc ggttaataag     4800

ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc     4860

gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat atccacaccc     4920

tcggattgcc gttcagtgaa gtggagcgga acgaccttac gaccgtcccg ctcacgaggc     4980

tttacgcact acgtactgcg atggcttcaa tttccagcgg gagggcggat ccactaatac     5040

aaaatatatc aaaagttaat aataatatta ttcttactta agactttttt gtcttcattt     5100

tttagtaaaa aatataaaaa aggccacctc ccgattttat cggaaggcag cctcttaaat     5160

tcagttcata atattaaaaa atattattca acttcagaat atttgttggc ataggcagct     5220

gccgcaccca acagtccagg ctgcggataa gtaatcaact taaccggaat cttggacatg     5280

acgcgttcaa agcgtccttt tgaaacaaag cgctgacgga aaccagattc tggcaaatgg     5340

gaagcgatac gaagaccgac accaccgcca ataacaacac tggttcgacc ctgtgccaaa     5400

gcaagatcac cagcgatagc gccaaggctc aagcagaagc gatccaaagc ggcttcagca     5460

aggttgtctt taccttccaa agccatctgc cataatttaa tatcatccag caagctgaac     5520

ggaacgcctt caatggcagc cagtgcttcg tagatattac caagacccgg gccagaaata     5580

atgcgttcga tagaaacgcg gcggaaacgt tcacgtaaac gtgccagaat tttgtcttca     5640

agtctgtcaa gcggagcaaa gtcgatatga ccgccttcag tttcgatgac gaaataacgg     5700

ccttcagtcc gcaacagatg ggcaacaccc aagcccgttc ccggaccaag aatagtgata     5760

acaccatcgc taggaagcgc ttcatcagga ccacaaatat gatccagata agaagaatcc     5820

atatgcgcaa ccgcgtgggc aaccgcgccg aagtcattga tcagaacatg cgtatcgatg     5880

tccagctttt cattcagagt agctggtctt aatacccaag ggttattggt aagttttaaa     5940

acttcaccat gaaccgggcc agcccatgca atagctgcgg cacgtggcag aggacgaccc     6000

agtttttcac cgaaacgttc ccaagctaac tgcaagctag catgttctgc cgttttaaaa     6060

gttgtttctt ctccaagaga aagaacccga ccattgctta cttccgcaat agagaaacgc     6120

gcatgcgttc caccgatgtc aatcgcaaca atttccataa taattccttt ctgaaatcag     6180

aaggctaccc aacaggtaaa ataagtccgc ccgctttata ccatcgttgt aaacaaaaag     6240

tataattggt taagacttat ctaaaaaaga caaaaggatt cagccaaagc aagtttaact     6300

acttctggga gcgccacatc tcctcgattt catccaggct ccgacctttg gtttccggca     6360

cgaagcgagc aacaatcaag ccacctaaga tacttaatgc tgcgaaaacg agataggaga     6420

aaccgtggtt gaaagtctga ttcaatgctg gagaaccatc ggcaacctta aacaggaagt     6480

taaccaagat attagctaac cattgtccgg taacagcgat aggcatagct gcgcccttga     6540

tggaactcgg gaacatttct gacagaacaa cccagcagac agggccccat gacataccaa     6600

agactgcaat ataaagaagc acagaagcca aaggcaaaac accaccgact ttgaaccaga     6660

aacagcagcc taaaacagcc atcattgcag ccataccgag agcaccccaa ataagcagag     6720

gtttacggcc gaagcggtca acaacacggg aagcaatcat ggtgaagatg aagttcacaa     6780

caccgataga gatggtctgc aataatgccg tatcagctcc aaaacctaaa ttctggaaca     6840

tctgcggtgc ataatacagc acggcgttaa taccgactaa ctgctggaag gcagcaacgg     6900

atacaccggc aaaaacaacg gtgataccaa aagcaaacaa acctgcgctg cttttgtcca     6960

tggctttatc aaagccagct ttaatctttt gaatcgtcag attaggatcg gcttgcggtt     7020

ccagacgagc aaggattttg ctagcctcgg aatgacgtcc cttcatcacc aaccaatgcg     7080

gcgtatccgg tgcggttaac agcagcaata agaaggcaat accgatcagg ccttctgaag     7140

ccggagacca gcaccaacca ctggcattaa cccaatcgat agaaccgaaa tgagccagta     7200

accaggtaaa gatataaccg gttaaagcac ccgtcacaat ggccatctgc tgaccagaaa     7260

ccatctgacc acgtttgtct ggcggagcaa tttcagcaat ataggttggg gtcaaggttg     7320

aaacgacacc gatacctaaa ccggcaagaa accggaaaaa gcaaaaaatt tgtaaagccg     7380

aaccaccggt tccaaataat ttttcggtta acgcagcacc aaaaccggcg gcgacgaaac     7440

aaatggaact catcaacaat ccgccgcgac gaccgaagcg aataccaatc cagccagaca     7500

gcaaagaacc ggtaacacaa ccgaccaaaa cagcaacaac gaccatccca gaaagggaag     7560

ccgcagccgt agcagacagg tgacgagggg caataaaatg gatatcaacc ggtgtaccga     7620

ttgcagcgat aaccgctgaa tcgtaaccga aaagcaagcc gcctatagca gcgattaggg     7680

ctagtcgcgt gactagaccc tgactacttt cagaactcat ggcgattcct ctccctctag     7740

agcgtcctgc tgttgttaag attattatac cacaccttgt agataaagtc aacaactttt     7800

tgcaaaattt ttcaggaatt ttagcagagg ttgttctgga tgtagaacaa aacatctttc     7860

cgctcttgtg ctgttaggat atctttcttg gaagctaggt aggcctcgag ttatggcagt     7920

tggttaaaag gaaacaaaaa gaccgttttc acacaaaacg gtctttttcg atttcttttt     7980

acagtcacag ccacttttgc accaattaag gccacgctgt catttaaact ccgtttttcc     8040

agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc     8100

tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac     8160

attaatactt cacagtcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag     8220

ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta     8280

aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt     8340

atccagacga ccacggtatt tcgccagaga cggcgcttta cggatggcat acatcggcaa     8400

caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt cgaggatgtt     8460

cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgagagtatt     8520

cagtttaccc agcgacactt tggttttgtc acctttataa ccgaacttca gaatcagacc     8580

attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag     8640

tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttgg taccggtcat     8700

ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc     8760

catgttggcc ggagacagag taaacaggca gctaaaggca aagaacatca ccactgcaac     8820

catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc     8880

gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa     8940

gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg     9000

accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg     9060

gatcagcgac aaagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcag     9120

ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg     9180

cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga     9240

agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct     9300

gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct     9360

cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc     9420

tgacgaattc                                                            9430


<210>  2
<211>  9462
<212>  DNA
<213>  Escherichia coli


<220>
<221>  pAC21
<222>  (1)..(9462)
<223>  Pplasmid used to install a slectable and counterselectable 
       cat-sacB cassette at the tdc locus of strain KJ122

<220>
<221>  pAC21
<222>  (1)..(9462)
<223>  Plasmid used to install a slectable and counterselectable 
       cat-sacB cassette at the tdc locus of strain KJ122

<220>
<221>  misc_feature
<222>  (5924)..(5924)
<223>  n is a, c, g, or t

<400>  2
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc       60

agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag      120

gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc      180

tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa      240

aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg      300

aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca      360

gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt      420

gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta      480

gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc      540

tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta      600

cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac      660

ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata      720

gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa      780

atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct      840

gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt      900

tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat      960

tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta     1020

aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat     1080

tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata     1140

atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat     1200

taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat     1260

ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg     1320

ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata     1380

agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc     1440

tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca     1500

ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg     1560

caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg     1620

atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac     1680

tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca     1740

agactaacaa acaaaagtag aacaactgtt caccgttaca tatcaaaggg aaaactgtcc     1800

atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt     1860

ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta     1920

acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac     1980

ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg     2040

cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa     2100

tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga     2160

ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg     2220

cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca     2280

attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc     2340

cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc     2400

ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt     2460

gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct     2520

cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc     2580

gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct     2640

tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc     2700

gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt     2760

gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc     2820

gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac     2880

gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg     2940

tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga     3000

tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca     3060

tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca     3120

tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg     3180

gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg     3240

aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga     3300

gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc     3360

gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag     3420

gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag     3480

aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac     3540

aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg     3600

ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg     3660

gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt     3720

atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg     3780

cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg     3840

tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta     3900

actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc     3960

tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg     4020

aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta     4080

aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat     4140

agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt     4200

ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg     4260

tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat     4320

taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc     4380

ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg     4440

caggtcgact ctagaggatc ccccccgccg ccgacagagt aataggtttt acttaatagc     4500

tcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg     4560

ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact     4620

ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca     4680

tcctgcggac tgtttcctgc cagtcccatg atgatggcga catccgtggc atggcctttg     4740

cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc ggttaataag     4800

ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc     4860

gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat gtgacggaag     4920

atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca     4980

acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg     5040

aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag     5100

ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta     5160

aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc     5220

tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct     5280

ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag     5340

acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa     5400

ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca     5460

tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta     5520

ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa     5580

acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc     5640

aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct     5700

tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg     5760

cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag     5820

tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca     5880

gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttantcggta cccggggatc     5940

gcggccgcgg accggatccc atcacatata cctgccgttc actattattt agtgaaatga     6000

gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca tgcaacagaa     6060

actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct ttaggcccgt     6120

agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga ccagttgcaa     6180

tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat     6240

aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg tcacccctta     6300

catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa caggagggct     6360

ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat gaacatcaaa     6420

aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc aggaggcgca     6480

actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata cggcatttcc     6540

catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa     6600

gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg cctggacgtt     6660

tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca cggctaccac     6720

atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat ttacatgttc     6780

tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg cgtctttaaa     6840

gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca agaatggtca     6900

ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga tttctccggt     6960

aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc atcagacagc     7020

tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga cggaaaaacg     7080

tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga caaccatacg     7140

ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt tgaagcaaac     7200

actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc atactatggc     7260

aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc     7320

acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga ttacacactg     7380

aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat tgaacgcgcg     7440

aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg atcaaaaatg     7500

acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc taattcttta     7560

actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga tcttgatcct     7620

aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc     7680

gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc aacgtttgcg     7740

ccgagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt     7800

gaacaaggac aattaacagt taacaaataa aaacgcaaaa gaaaatgcca atatcctatt     7860

ggcattttct tttatttctt ccatttaaat ggatgcatgc gctagcggag tgtatactgg     7920

cttactatgt tggcactgat gagggtgtca gtgaagtgct tcagcctcgt gagcgggacg     7980

gtcgtaaggt cgttccgctc cacttcactg aacggcaatc cgagggtgtg gatccaatta     8040

aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac     8100

cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat     8160

tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacagtcg ctggcatcga     8220

gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat     8280

tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg     8340

gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga     8400

gacggcgctt tacggatggc atacatcggc aacaggcaca gcagggatgc gataatcggt     8460

gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg     8520

gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg     8580

tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag     8640

tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt     8700

gtaatcgcga acgttgtttt ggtaccggtc atggacgcaa agtggttagc cagataagaa     8760

agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg     8820

cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt     8880

tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta     8940

gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc     9000

cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca     9060

acctggtcga taactgcaga gttccagtaa gggatcagcg acaaagaaat cagcaccagg     9120

ctggcgataa acggccatac caggtagctc agggtaccga gctcgaattc actggccgtc     9180

gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca     9240

catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa     9300

cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg     9360

tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag     9420

ttaagccagc cccgacaccc gccaacaccc gctgacgaat tc                        9462


<210>  3
<211>  1091
<212>  DNA
<213>  Escherichia coli


<220>
<221>  ptsHI
<222>  (1)..(1091)
<223>  Sequence surrounding the deletion of ptsHI. The fusion junction 
       is in between the nucleotide positions 425 and 426.

<400>  3
gcaacagtaa tgccagcttg ttaaaaatgc gtaaaaaagc acctttttag gtgctttttt       60

gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt      120

taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa      180

tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa      240

acgtcagcgg tcaacacccg ccagcaatgg actgtattgc gctcttcgtg cgtcgcgtct      300

gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc      360

cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc      420

gctccacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga gaagatcatg      480

ggtttgttcg ataaactgaa atctctggtt tccgacgaca agaaggatac cggaactatt      540

gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc ggatgtcgtt      600

tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa caaaatggtc      660

gcgccagtag acggcaccat tggtaaaatc tttgaaacca accacgcatt ctctatcgaa      720

tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga actgaaaggc      780

gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga tactgtcatt      840

gaatttgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc ggttgttatc      900

tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac cgtgggtgaa      960

accccggtta tccgcatcaa gaagtaattc tgccgcagtg aaaaatggcg cccatcggcg     1020

ccattttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc atgctgctgg     1080

gtgtagcgca t                                                          1091


<210>  4
<211>  66
<212>  DNA
<213>  Escherichia coli


<220>
<221>  galP
<222>  (1)..(66)
<223>  Sequence surrounding the deletion of galP gene. The fusion 
       junction is in between the nucelotide positions 48 and 49.

<400>  4
atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgtttat aggcgctcac       60

gattaa                                                                  66


<210>  5
<211>  8446
<212>  DNA
<213>  Escherichia coli


<220>
<221>  pSS2
<222>  (1)..(8446)
<223>  Plasmid used to install a glf cassette at the tdc lcous of 
       bacterial strain KJ122.

<400>  5
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc       60

agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag      120

gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc      180

tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa      240

aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg      300

aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca      360

gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt      420

gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta      480

gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc      540

tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta      600

cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac      660

ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata      720

gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa      780

atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct      840

gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt      900

tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat      960

tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta     1020

aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat     1080

tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata     1140

atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat     1200

taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat     1260

ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg     1320

ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata     1380

agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc     1440

tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca     1500

ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg     1560

caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg     1620

atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac     1680

tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca     1740

agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc     1800

atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt     1860

ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta     1920

acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac     1980

ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg     2040

cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa     2100

tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga     2160

ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg     2220

cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca     2280

attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc     2340

cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc     2400

ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt     2460

gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct     2520

cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc     2580

gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct     2640

tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc     2700

gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt     2760

gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc     2820

gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac     2880

gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg     2940

tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga     3000

tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca     3060

tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca     3120

tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg     3180

gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg     3240

aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga     3300

gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc     3360

gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag     3420

gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag     3480

aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac     3540

aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg     3600

ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg     3660

gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt     3720

atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg     3780

cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg     3840

tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta     3900

actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc     3960

tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg     4020

aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta     4080

aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat     4140

agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt     4200

ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg     4260

tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat     4320

taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc     4380

ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg     4440

caggtcgact ctagaggatc ccccccgccg ccgacagagt aataggtttt acttaatagc     4500

tcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg     4560

ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact     4620

ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca     4680

tcctgcggac tgtttcctgc cagtcccatg atgatggcga catccgtggc atggcctttg     4740

cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc ggttaataag     4800

ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc     4860

gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat atccacaccc     4920

tcggattgcc gttcagtgaa gtggagcgga acgaccttac gaccgtcccg ctcacgaggc     4980

tttacgcact acgtactgcg atggcttcaa tttccagcgg gagggcggat ccactaatac     5040

aaaatatatc aaaagttaat aataatatta ttcttactta agactttttt gtcttcattt     5100

tttagtaaaa aatataaaaa aggccacctc ccgattttat cggaaggcag cctcttaaat     5160

tcagttcata atattaaaaa atattattca acttcagatt atttgttggc ataggcagcg     5220

ctccgcccgc tttataccat cgttgtaaac aaaaagtata attggttaag acttatctaa     5280

aaaagacaaa aggattcagc caaagcaagt ttaactactt ctgggagcgc cacatctcct     5340

cgatttcatc caggctccga cctttggttt ccggcacgaa gcgagcaaca atcaagccac     5400

ctaagatact taatgctgcg aaaacgagat aggagaaacc gtggttgaaa gtctgattca     5460

atgctggaga accatcggca accttaaaca ggaagttaac caagatatta gctaaccatt     5520

gtccggtaac agcgataggc atagctgcgc ccttgatgga actcgggaac atttctgaca     5580

gaacaaccca gcagacaggg ccccatgaca taccaaagac tgcaatataa agaagcacag     5640

aagccaaagg caaaacacca ccgactttga accagaaaca gcagcctaaa acagccatca     5700

ttgcagccat accgagagca ccccaaataa gcagaggttt acggccgaag cggtcaacaa     5760

cacgggaagc aatcatggtg aagatgaagt tcacaacacc gatagagatg gtctgcaata     5820

atgccgtatc agctccaaaa cctaaattct ggaacatctg cggtgcataa tacagcacgg     5880

cgttaatacc gactaactgc tggaaggcag caacggatac accggcaaaa acaacggtga     5940

taccaaaagc aaacaaacct gcgctgcttt tgtccatggc tttatcaaag ccagctttaa     6000

tcttttgaat cgtcagatta ggatcggctt gcggttccag acgagcaagg attttgctag     6060

cctcggaatg acgtcccttc atcaccaacc aatgcggcgt atccggtgcg gttaacagca     6120

gcaataagaa ggcaataccg atcaggcctt ctgaagccgg agaccagcac caaccactgg     6180

cattaaccca atcgatagaa ccgaaatgag ccagtaacca ggtaaagata taaccggtta     6240

aagcacccgt cacaatggcc atctgctgac cagaaaccat ctgaccacgt ttgtctggcg     6300

gagcaatttc agcaatatag gttggggtca aggttgaaac gacaccgata cctaaaccgg     6360

caagaaaccg gaaaaagcaa aaaatttgta aagccgaacc accggttcca aataattttt     6420

cggttaacgc agcaccaaaa ccggcggcga cgaaacaaat ggaactcatc aacaatccgc     6480

cgcgacgacc gaagcgaata ccaatccagc cagacagcaa agaaccggta acacaaccga     6540

ccaaaacagc aacaacgacc atcccagaaa gggaagccgc agccgtagca gacaggtgac     6600

gaggggcaat aaaatggata tcaaccggtg taccgattgc agcgataacc gctgaatcgt     6660

aaccgaaaag caagccgcct atagcagcga ttagggctag tcgcgtgact agaccctgac     6720

tactttcaga actcatggcg attcctctcc ctctagagcg tcctgctgtt gttaagatta     6780

ttataccaca ccttgtagat aaagtcaaca actttttgca aaatttttca ggaattttag     6840

cagaggttgt tctggatgta gaacaaaaca tctttccgct cttgtgctgt taggatatct     6900

ttcttggaag ctaggtaggc ctcgagttat ggcagttggt taaaaggaaa caaaaagacc     6960

gttttcacac aaaacggtct ttttcgattt ctttttacag tcacagccac ttttgcacca     7020

attaaggcca cgctgtcatt taaactccgt ttttccagtt caaatgcaat tgccttcaat     7080

gcaccttcgt agctgtggtg agccagcggt gctggctctc ccccatttac ggataagaat     7140

gcattttccg agttaatacc gtcggcaata cctgacatta atacttcaca gtcgctggca     7200

tcgagtacgg aaaacttaat cgaagacgaa ccacagttaa taaccaaaac aaccggaaat     7260

tcattcatct cttttctcat cctgagttac ggattaaaac agtttgtata cgatgttcag     7320

gatggtcagc agaccaatca cggtaacaaa cacgttatcc agacgaccac ggtatttcgc     7380

cagagacggc gctttacgga tggcatacat cggcaacagg cacagcaggg atgcgataat     7440

cggtgcgccc atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg caacaaccca     7500

ggtggagccc atgatgaaga tcatgctgag agtattcagt ttacccagcg acactttggt     7560

tttgtcacct ttataaccga acttcagaat cagaccattc aagccttcca gcgtccccag     7620

atagtgaccg aagaaagatt tgaagatagc cacgagtgcg atgatggaag ccgcatattc     7680

cagtgtaatc gcgaacgttg ttttggtacc ggtcatggac gcaaagtggt tagccagata     7740

agaaagcact ggaatattct gcgctttggc ttccgccatg ttggccggag acagagtaaa     7800

caggcagcta aaggcaaaga acatcaccac tgcaaccatc agcatgctgg cacgagaaat     7860

gatttgggaa catttacgtt cggtgaagtc gcgaccgaag tctttctcat actcttcacg     7920

tttagaaacc acgaaggaag agacgattgg cgagaagtta aaggagaaaa ccatgatgga     7980

aatccccagc cagacagtga tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag     8040

gtcaacctgg tcgataactg cagagttcca gtaagggatc agcgacaaag aaatcagcac     8100

caggctggcg ataaacggcc ataccaggta gctcagggta ccgagctcga attcactggc     8160

cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc     8220

agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc     8280

ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca     8340

tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc     8400

atagttaagc cagccccgac acccgccaac acccgctgac gaattc                    8446


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer BY249 for amplifying and cloning the crr gene of 
       YSS41, together with BY249.

<400>  6
gggcaacagt aatgccagct tgttaaaaat g                                      31


<210>  7
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer BY250 for amplifying and cloning the crr gene of YSS41
       together with BY249.

<400>  7
gggcgctaca cccagcagca tgaga                                             25


<210>  8
<211>  8937
<212>  DNA
<213>  Escherichia coli


<220>
<221>  pMH68
<222>  (1)..(8937)
<223>  Plasmid used to install a second copy of teh crr gene from YSS41 
       at the pflD locus.

<400>  8
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc       60

agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag      120

gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc      180

tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa      240

aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg      300

aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca      360

gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt      420

gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta      480

gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc      540

tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta      600

cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac      660

ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata      720

gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa      780

atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct      840

gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt      900

tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat      960

tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta     1020

aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat     1080

tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata     1140

atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat     1200

taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat     1260

ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg     1320

ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata     1380

agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc     1440

tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca     1500

ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg     1560

caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg     1620

atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac     1680

tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca     1740

agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc     1800

atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt     1860

ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga acagcatgta     1920

acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga aattgaacac     1980

ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca ggcgatgctg     2040

cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa     2100

tcatggcaat tctggaagaa atagcgcttt cagccggcaa acctgaagcc ggatctgcga     2160

ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgttatg     2220

cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca     2280

attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc     2340

cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc     2400

ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt     2460

gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct     2520

cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc     2580

gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct     2640

tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc     2700

gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt     2760

gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc     2820

gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac     2880

gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg     2940

tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga     3000

tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca     3060

tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca     3120

tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg     3180

gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg     3240

aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga     3300

gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc     3360

gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag     3420

gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag     3480

aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac     3540

aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg     3600

ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg     3660

gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt     3720

atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg     3780

cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg     3840

tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta     3900

actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc     3960

tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg     4020

aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta     4080

aaatctagcg agggctttac taagctgatc cggtggatga ccttttgaat gacctttaat     4140

agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt     4200

ttcacaaaac ggtttacaag catacgttgg ccgattcatt aatgcagctg gcacgacagg     4260

tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat     4320

taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagt     4380

gccaataccc gtgaaatctc gctggagcgg gcgctgcttt ataccgccag ccatcggcaa     4440

accgaaggcg aaccggtgat attgcgccgg gcgaaagcaa cagcgtatat ccttgaacat     4500

gttgaaattt cgattcgtga tgaagaactg attgccggta accgcaccgt aaaaccgcgc     4560

gccgggatta tgtcgccgga aatggaccct tactggctgc tgaaagagct ggatcaattc     4620

ccgacgcgtc cgcaggaccg ctttgctatc agcgaagaag ataaacgtat ctaccgcgaa     4680

gagttgttcc cgtactggga aaaacgttcg atgaaagatt tcatcaacgg gcagatgaca     4740

gatgaagtaa aagccgcgac cagcacgcag attttcagca tcaaccagac agataaaggc     4800

caggggcaca ttattattga ttacccacgc ctgctgaatc acgggctggg ggagctggta     4860

gcacagatgc agcaacattg tcagcaacag ccggagaatc acttttatca ggcagcgctg     4920

ttactgctgg aagcctcgca gaaacacatt ttgcgttacg ccgaactggc ggaaacgatg     4980

gcggcaaact gcacagatgc ccagcgtcgc gaagagctgc tgactattgc ggagatctcc     5040

cgccataacg cgcaacataa gccgcagacg ttctggcagg cgtgccagtt attctggtac     5100

atgaacatca ttctgcaata cgaatccaac gccagttcgc tatcgttggg gcgcttcgac     5160

cagtatatgt tgccgttcta tcagacatca ttaacccagg gcgaagatgc ggcgttcctg     5220

aaagaactgc tcgaatcttt atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc     5280

agcagcgcgc gttatttcgc cggtttcccg accggctata ccgcactgct cggcgggtta     5340

accgagaacg gacgtagcgc ggtgaacgtg ctttcgttcc tttgccttga cgcctatcaa     5400

agcgtgcaat taccgcaacc gaacctcggc gtgcgcacta acgccttgat cgacacgccg     5460

ttcctgatga aaaccgccga aaccattcgc ctcggcaccg gtattccgca aatctttaac     5520

gatgaagtgg tggtgccagc gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac     5580

tattccgtag tgggctgtgt ggaattatct attcccggca gaacctacgg cttgcatgac     5640

atcgcgatgt ttaacctgct gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaat     5700

gccgcgctga cttatgaagg tttactggaa cagatccgtg ccaagatcag ccactacatc     5760

accctgatgg ttgaaggcag taatatttgc gatatcggcc atcgcgactg ggcacctgta     5820

ccgctgctct cgtcttttat cagcgattgt ctggaaaaag gccgcgatat taccgatggc     5880

ggcgcgcgtt ataacttctc cggcgtacag gggatcggta tcgccaacct gagcgattct     5940

ctccatgcgt tgaaagggat ggtttttgat caacagcgtt taagttttga cgaattgctg     6000

tcggtattaa aagccaactt tgcaacgcca gaaggcgaaa aagtccgcgc tcgcttaatt     6060

aaccgctttg agaaatacgg taacgatatc gacgaggtgg ataacattag cgccgaactg     6120

ttgcgccact actgcaaaga agtggaaaaa taccagaacc cgcgcggcgg ctacttcacg     6180

ccgggatcgt agggcttcaa actttcgccc ctcctggcat tgattcagcc tgtcggaact     6240

ggtatttaac cagactaatt attttgatgc gcgaaattaa tcgttacagg aaaagccaaa     6300

gctgaatcga ttttatgatt tggttcaatt cttcctttag cggcataatg tttaatgacg     6360

tacgaaacgt cagcggtcaa cacccgccag caatggactg tattgcgctc ttcgtgcgtc     6420

gcgtctgtta aaaactggcg ctaacaatac aggctaaagt cgaaccgcca ggctagactt     6480

tagttccaca acactaaacc tataagttgg ggaaatacaa tgttccagca agaagttacc     6540

attaccgctc cgacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa     6600

gatcatgggt ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg     6660

aactattgag atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga     6720

tgtcgttttt gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa     6780

aatggtcgcg ccagtagacg gcaccattgg taaaatcttt gaaaccaacc acgcattctc     6840

tatcgaatct gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact     6900

gaaaggcgaa ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac     6960

tgtcattgaa tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt     7020

tgttatctcc aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt     7080

gggtgaaacc ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc     7140

catcggcgcc atttttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat     7200

gctgctgggt gtagcgccct accgtttctg ctcacgttcc gttgggatcg gtggttggcg     7260

cgacgccaga cggtcgtttt gccggagaac agctggcaga cggcggcttg tcacctatgc     7320

tgggtcagga cgcacaaggg ccaacggcgg tactgaagtc agtcagtaag ctcgataaca     7380

cactgctgtc taacggtaca ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg     7440

aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt aagttacaac     7500

atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag cgcccacaag     7560

attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc gaactgtcga     7620

aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt tgtggaaacg     7680

cgccgcaatg atgtggcgcg cattttcaac attcagcgtt attcactgaa tgacggtgag     7740

ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg gtgtgctaat     7800

ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg tctgcactgt     7860

gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg gattggtcgc     7920

gatatcagcc ttgacgctct ggaacgggaa gtgatgaaag atgacatttt ttttcgcacg     7980

tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc     8040

cgttttttac agcgactgca gctgtggggt gtctcatgtg ccattgaaac tgccggagac     8100

gcgccagcca gcaagctgtt accgctggcg aaattgtgcg atgaagtgtt gttcgattta     8160

aaaatcatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc acgcgtgctg     8220

gagaatctgc gtttgctggt gagtgagggc gtcaacgtga tcccgcgttt accgctgatc     8280

cctggtttca cgctcagccg ggagaatatg cagcaggcgc tggatgtgct gatcccgctg     8340

aatatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa ataccgcctg     8400

ctggggaaaa catggtcgat gaaagaggtg cctgcgccgt cgtcagccga tgtggcaacg     8460

atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta aaatggcata     8520

cctggtggca gtaaccgcct gcgtcagtgg cgtggcgcat acttatatgg cggcggaacg     8580

gctggaaaag ttgtgcctgt tagagaagtg gggagtcagc attgaaactc agggcgctcg     8640

aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt     8700

aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc     8760

gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt     8820

ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc     8880

tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgaattc        8937


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer BY124 for amplifying and cloning teh pflDC geen of 
       Escherichia coli C, or cassettes embedded in pflD, together with 
       BY125.

<400>  9
tgccaatacc cgtgaaatct cgc                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer BY125 for amplifying and cloning the pflDC genes of E.
       coli C, or cassettes embedded in pflD, together with BY124.

<400>  10
cgccctgagt ttcaatgctg act                                               23


<210>  11
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer AC74 for amplifying and clonig the tdcC-tdcG region of
       E. coli C and derivatives or cassettes embedded in teh tdcC-tdcG 
       region,together with AC75.

<400>  11
tgagctacct ggtatggccg tttatc                                            26


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer Ac75 for amplifying and cloning the tdcC-tdcG region 
       of E. coli C and derivatives, or cassettes embedded in teh 
       tdcC-tdcG region, together with AC74.

<400>  12
cccgccgccg acagagtaat ag                                                22


