                         SEQUENCE LISTING

<110>  NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY
 
<120>  ASCORBATE TRANSPORTER

<130>  OP00867PCT

<150>  JP 2013-155640
<151>  2013-07-26

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  2128
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  CDS
<222>  (247)..(1872)

<400>  1
gaaatgagaa cagaacggag gttccaacga gtagaagatg atgtgccacg tgtcactata       60

tcgacccccc tttcctcttc cttccttccc caatagagag ggattttccc gcttcagcgt      120

ctctcagtct ctctctcggc tttatccaaa ggttccttcg tttactccgc ttctatggaa      180

gaagcttgaa tccttctctc ttcttcttct ccttcttccc ttgacatttt tgttctgaga      240

agcaac atg gcc ctc ggt ggc ttg att tca aat cgg aac ttc ggt tcc         288
       Met Ala Leu Gly Gly Leu Ile Ser Asn Arg Asn Phe Gly Ser            
       1               5                   10                             

ttc atc ggc tca ggc aat ggt tgt cag aga ctt gga aaa agt ggt gca        336
Phe Ile Gly Ser Gly Asn Gly Cys Gln Arg Leu Gly Lys Ser Gly Ala           
15                  20                  25                  30            

gag gtt tcc aag ctt ttt cca aat gca ttg ttg tgc cgt aat cat caa        384
Glu Val Ser Lys Leu Phe Pro Asn Ala Leu Leu Cys Arg Asn His Gln           
                35                  40                  45                

cct ctg caa gct agt tta cat cat gaa tcg gga cac atg aga aga agt        432
Pro Leu Gln Ala Ser Leu His His Glu Ser Gly His Met Arg Arg Ser           
            50                  55                  60                    

ttt gga tgt ttc ctt caa cca aga atg gac agt gta att agg ttt agg        480
Phe Gly Cys Phe Leu Gln Pro Arg Met Asp Ser Val Ile Arg Phe Arg           
        65                  70                  75                        

aac agt atc aag atc aac aga tct cgt gct tac tac aaa tca gag gag        528
Asn Ser Ile Lys Ile Asn Arg Ser Arg Ala Tyr Tyr Lys Ser Glu Glu           
    80                  85                  90                            

tca gat atc acg gaa gga gtt gtg cca tcg gct gat ggc tca gct gaa        576
Ser Asp Ile Thr Glu Gly Val Val Pro Ser Ala Asp Gly Ser Ala Glu           
95                  100                 105                 110           

gct att tta gtt gaa gga aat cta cag aac gca tct cca tgg tgg cag        624
Ala Ile Leu Val Glu Gly Asn Leu Gln Asn Ala Ser Pro Trp Trp Gln           
                115                 120                 125               

cag ttt cct aga cgc tgg gtg atc gtc ttg tta tgc ttt tcg tct ttt        672
Gln Phe Pro Arg Arg Trp Val Ile Val Leu Leu Cys Phe Ser Ser Phe           
            130                 135                 140                   

ctt ctt tgc aat atg gac cgt gtg aac atg agc att gca att ctt ccc        720
Leu Leu Cys Asn Met Asp Arg Val Asn Met Ser Ile Ala Ile Leu Pro           
        145                 150                 155                       

atg tca caa gaa tat aac tgg agt agt gca act gtt ggc tta att cag        768
Met Ser Gln Glu Tyr Asn Trp Ser Ser Ala Thr Val Gly Leu Ile Gln           
    160                 165                 170                           

tcc tct ttc ttc tgg ggc tat tta ctt act cag att ctg gga ggt ata        816
Ser Ser Phe Phe Trp Gly Tyr Leu Leu Thr Gln Ile Leu Gly Gly Ile           
175                 180                 185                 190           

tgg gca gat aag ttt ggc ggg aag gtt gtt tta ggt ttt ggt gtt gtc        864
Trp Ala Asp Lys Phe Gly Gly Lys Val Val Leu Gly Phe Gly Val Val           
                195                 200                 205               

tgg tgg tcc ttt gcc act att atg aca cct att gct gct aga ctt ggt        912
Trp Trp Ser Phe Ala Thr Ile Met Thr Pro Ile Ala Ala Arg Leu Gly           
            210                 215                 220                   

ctt cct ttt ttg ctt gta gtg cgg gcg ttc atg ggc att ggc gag ggt        960
Leu Pro Phe Leu Leu Val Val Arg Ala Phe Met Gly Ile Gly Glu Gly           
        225                 230                 235                       

gtt gca atg cct gct atg aac aac atg ctt tct aaa tgg atc cct gtc       1008
Val Ala Met Pro Ala Met Asn Asn Met Leu Ser Lys Trp Ile Pro Val           
    240                 245                 250                           

tca gag aga agc aga tcg ctt gca ctg gtc tat agt ggc atg tat ctt       1056
Ser Glu Arg Ser Arg Ser Leu Ala Leu Val Tyr Ser Gly Met Tyr Leu           
255                 260                 265                 270           

ggt tcc gtc aca gga ttg gca ttc tct cct atg cta atc acc aag ttc       1104
Gly Ser Val Thr Gly Leu Ala Phe Ser Pro Met Leu Ile Thr Lys Phe           
                275                 280                 285               

gga tgg cca tct gtt ttc tac tcg ttt ggc tcc ctc gga agt ata tgg       1152
Gly Trp Pro Ser Val Phe Tyr Ser Phe Gly Ser Leu Gly Ser Ile Trp           
            290                 295                 300                   

ttt ttg cta tgg cta aaa ttt gca tat agc tct ccc aag gat gac ccg       1200
Phe Leu Leu Trp Leu Lys Phe Ala Tyr Ser Ser Pro Lys Asp Asp Pro           
        305                 310                 315                       

gac cta agt gaa gaa gaa aaa aag gtt ata ctg gga ggt agc aaa cca       1248
Asp Leu Ser Glu Glu Glu Lys Lys Val Ile Leu Gly Gly Ser Lys Pro           
    320                 325                 330                           

agg gaa cct gtt aca gtg att cca tgg aag cta ata ctg tct aaa ccc       1296
Arg Glu Pro Val Thr Val Ile Pro Trp Lys Leu Ile Leu Ser Lys Pro           
335                 340                 345                 350           

cca gtc tgg gct ctc ata att tcc cac ttc tgc cat aat tgg gga act       1344
Pro Val Trp Ala Leu Ile Ile Ser His Phe Cys His Asn Trp Gly Thr           
                355                 360                 365               

ttc ata cta ttg aca tgg atg ccc aca tat tac aat cag gtc ttg aag       1392
Phe Ile Leu Leu Thr Trp Met Pro Thr Tyr Tyr Asn Gln Val Leu Lys           
            370                 375                 380                   

ttc aac ctc act gaa tcc ggg cta cta tgc gtt ttg cca tgg cta acc       1440
Phe Asn Leu Thr Glu Ser Gly Leu Leu Cys Val Leu Pro Trp Leu Thr           
        385                 390                 395                       

atg gct gtt ttt gct aat att gga ggt tgg att gct gat act ctt gtg       1488
Met Ala Val Phe Ala Asn Ile Gly Gly Trp Ile Ala Asp Thr Leu Val           
    400                 405                 410                           

agc aga ggt ctt tca att aca aac gtt cga aag att atg caa tca att       1536
Ser Arg Gly Leu Ser Ile Thr Asn Val Arg Lys Ile Met Gln Ser Ile           
415                 420                 425                 430           

ggt ttt cta ggc cct gcc ttc ttc ctg agt cag ctg agc cat gtc aaa       1584
Gly Phe Leu Gly Pro Ala Phe Phe Leu Ser Gln Leu Ser His Val Lys           
                435                 440                 445               

act cca gca atg gca gtt ctc tgc atg gca tgc agt cag ggc tct gat       1632
Thr Pro Ala Met Ala Val Leu Cys Met Ala Cys Ser Gln Gly Ser Asp           
            450                 455                 460                   

gca ttc tct cag tcg ggt ctc tac tct aat cat caa gac att ggc cca       1680
Ala Phe Ser Gln Ser Gly Leu Tyr Ser Asn His Gln Asp Ile Gly Pro           
        465                 470                 475                       

cga tac gct ggt gta ctc tta gga cta tca aac aca gca gga gtc cta       1728
Arg Tyr Ala Gly Val Leu Leu Gly Leu Ser Asn Thr Ala Gly Val Leu           
    480                 485                 490                           

gct ggt gtc ttt ggc act gca gct act ggt tac atc ctc caa cga ggt       1776
Ala Gly Val Phe Gly Thr Ala Ala Thr Gly Tyr Ile Leu Gln Arg Gly           
495                 500                 505                 510           

tca tgg gat gat gtg ttc aaa gta gca gtt gca ctg tat cta att gga       1824
Ser Trp Asp Asp Val Phe Lys Val Ala Val Ala Leu Tyr Leu Ile Gly           
                515                 520                 525               

act ctg gtc tgg aac ttg ttc gca acc gga gaa aaa att ctc gac tag       1872
Thr Leu Val Trp Asn Leu Phe Ala Thr Gly Glu Lys Ile Leu Asp               
            530                 535                 540                   

aaccaagaaa ctaatagctg cagtgaaatt gtgtgaggaa gctttatatt tgttctttct     1932

ctgcttataa atttgatctg atagtgagat caaaatggtt cgctggtctg tttcataaag     1992

taacatgtta aagaaaagat gtattttttt ggaacttgac tcttttgaag cttacaattt     2052

gatggggatg gagaaaaaga aaccaaaaca aaaacaatca aactttttta ctttaggaag     2112

aagagtattt gcttac                                                     2128


<210>  2
<211>  541
<212>  PRT
<213>  Arabidopsis thaliana

<400>  2

Met Ala Leu Gly Gly Leu Ile Ser Asn Arg Asn Phe Gly Ser Phe Ile 
1               5                   10                  15      


Gly Ser Gly Asn Gly Cys Gln Arg Leu Gly Lys Ser Gly Ala Glu Val 
            20                  25                  30          


Ser Lys Leu Phe Pro Asn Ala Leu Leu Cys Arg Asn His Gln Pro Leu 
        35                  40                  45              


Gln Ala Ser Leu His His Glu Ser Gly His Met Arg Arg Ser Phe Gly 
    50                  55                  60                  


Cys Phe Leu Gln Pro Arg Met Asp Ser Val Ile Arg Phe Arg Asn Ser 
65                  70                  75                  80  


Ile Lys Ile Asn Arg Ser Arg Ala Tyr Tyr Lys Ser Glu Glu Ser Asp 
                85                  90                  95      


Ile Thr Glu Gly Val Val Pro Ser Ala Asp Gly Ser Ala Glu Ala Ile 
            100                 105                 110         


Leu Val Glu Gly Asn Leu Gln Asn Ala Ser Pro Trp Trp Gln Gln Phe 
        115                 120                 125             


Pro Arg Arg Trp Val Ile Val Leu Leu Cys Phe Ser Ser Phe Leu Leu 
    130                 135                 140                 


Cys Asn Met Asp Arg Val Asn Met Ser Ile Ala Ile Leu Pro Met Ser 
145                 150                 155                 160 


Gln Glu Tyr Asn Trp Ser Ser Ala Thr Val Gly Leu Ile Gln Ser Ser 
                165                 170                 175     


Phe Phe Trp Gly Tyr Leu Leu Thr Gln Ile Leu Gly Gly Ile Trp Ala 
            180                 185                 190         


Asp Lys Phe Gly Gly Lys Val Val Leu Gly Phe Gly Val Val Trp Trp 
        195                 200                 205             


Ser Phe Ala Thr Ile Met Thr Pro Ile Ala Ala Arg Leu Gly Leu Pro 
    210                 215                 220                 


Phe Leu Leu Val Val Arg Ala Phe Met Gly Ile Gly Glu Gly Val Ala 
225                 230                 235                 240 


Met Pro Ala Met Asn Asn Met Leu Ser Lys Trp Ile Pro Val Ser Glu 
                245                 250                 255     


Arg Ser Arg Ser Leu Ala Leu Val Tyr Ser Gly Met Tyr Leu Gly Ser 
            260                 265                 270         


Val Thr Gly Leu Ala Phe Ser Pro Met Leu Ile Thr Lys Phe Gly Trp 
        275                 280                 285             


Pro Ser Val Phe Tyr Ser Phe Gly Ser Leu Gly Ser Ile Trp Phe Leu 
    290                 295                 300                 


Leu Trp Leu Lys Phe Ala Tyr Ser Ser Pro Lys Asp Asp Pro Asp Leu 
305                 310                 315                 320 


Ser Glu Glu Glu Lys Lys Val Ile Leu Gly Gly Ser Lys Pro Arg Glu 
                325                 330                 335     


Pro Val Thr Val Ile Pro Trp Lys Leu Ile Leu Ser Lys Pro Pro Val 
            340                 345                 350         


Trp Ala Leu Ile Ile Ser His Phe Cys His Asn Trp Gly Thr Phe Ile 
        355                 360                 365             


Leu Leu Thr Trp Met Pro Thr Tyr Tyr Asn Gln Val Leu Lys Phe Asn 
    370                 375                 380                 


Leu Thr Glu Ser Gly Leu Leu Cys Val Leu Pro Trp Leu Thr Met Ala 
385                 390                 395                 400 


Val Phe Ala Asn Ile Gly Gly Trp Ile Ala Asp Thr Leu Val Ser Arg 
                405                 410                 415     


Gly Leu Ser Ile Thr Asn Val Arg Lys Ile Met Gln Ser Ile Gly Phe 
            420                 425                 430         


Leu Gly Pro Ala Phe Phe Leu Ser Gln Leu Ser His Val Lys Thr Pro 
        435                 440                 445             


Ala Met Ala Val Leu Cys Met Ala Cys Ser Gln Gly Ser Asp Ala Phe 
    450                 455                 460                 


Ser Gln Ser Gly Leu Tyr Ser Asn His Gln Asp Ile Gly Pro Arg Tyr 
465                 470                 475                 480 


Ala Gly Val Leu Leu Gly Leu Ser Asn Thr Ala Gly Val Leu Ala Gly 
                485                 490                 495     


Val Phe Gly Thr Ala Ala Thr Gly Tyr Ile Leu Gln Arg Gly Ser Trp 
            500                 505                 510         


Asp Asp Val Phe Lys Val Ala Val Ala Leu Tyr Leu Ile Gly Thr Leu 
        515                 520                 525             


Val Trp Asn Leu Phe Ala Thr Gly Glu Lys Ile Leu Asp 
    530                 535                 540     


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
atggagatgc gttctgtaga tt                                                22


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
ggttccaacg agtagaagat ga                                                22


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
ccgtcccgca agttaaatat g                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
tacctcgggt tcgaaatcga t                                                 21


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
gacctgcctc atcatactcg                                                   20


<210>  8
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
ttcctcaatc tcatcttctt cc                                                22


