                         SEQUENCE LISTING

<110>  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
       INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE 
       (INSERM)
       UNIVERSITE DE RENNES 1
 
<120>  VECTORS FOR PRODUCING AND SECRETING SUBSTANCE OF INTEREST BY 
       BACTERIA AND APPLICATIONS THEREOF

<130>  366050D27981

<150>  EP 13306080.6
<151>  2013-07-25

<160>  71    

<170>  PatentIn version 3.5

<210>  1
<211>  99
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  1
atgtcaaaga cactctctcg gatcgcatcc gtcgcttcgg ttgccgcgct cgccggcagc       60

atcaccgtca tcgccgggca gaacgcgtcc gccgacagc                              99


<210>  2
<211>  90
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  2
gtgaagaacg gtctcaagac cctgctcatt ggtggagtcg gcatcgcgac ccttgcggtc       60

ggcggcgtcg gaactgccat cgcagacaat                                        90


<210>  3
<211>  105
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  3
atgcgatcca ccacgacgaa ggcgtttgcc ggtgtcgctg tgctggcgct ggcgcttgct       60

ggctgcggct cgaattcggg ctcgtccacc aagtcggccg acagc                      105


<210>  4
<211>  93
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  4
atgctcactc gcaagagagt ggttgcagcg ggagctgccg ccaccctgtc cctcacggcg       60

tttgccgggt tgcagcccgc cagcgccgcc acc                                    93


<210>  5
<211>  108
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  5
atgggattca gggttggccg tcgtcccctc atcggggcag ttctcgccgg gtcgatggca       60

acactcgtgg gctgttccac ctcgggtagc ggcagtggag cctccagc                   108


<210>  6
<211>  111
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  6
atgcaggccc tccaaggaag gcgccggtca cgacgggtga tggcggccgc ggtagcagcc       60

ctcaccgcca tgaccgtgct gccctcccag ctcaacgccg ttgctgcacc c               111


<210>  7
<211>  90
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  7
atgtccactg gccgcatgaa gttcatcaag ctggcagttc ctgtcatcgt tgcctgctgc       60

ttgacgccaa tggctgcctt agctgatgtg                                        90


<210>  8
<211>  114
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  8
atggcgatga gggcacgtca cggcgtcgtc cggcttggtc tggtctgtct caccgcattg       60

gcggtcttcg gcacggcaaa tgtgtcgggt caggttgcgg tgatggctga gggc            114


<210>  9
<211>  108
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  9
ttgaaccagg ccctgtcgac gatgcgcctg aagatcggcg actccaccaa gcgcatccgg       60

atcttcttcg tcgtgatggc cgtggcgatc accctgctcg cgggacgg                   108


<210>  10
<211>  75
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  10
atgcgacgtc gcaccacgat tgcagccctc gctgctgtct tgagtttcag tcccctggcc       60

gcccaggccg caccc                                                        75


<210>  11
<211>  132
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  11
atgcctagtc atgcggtgcg ggagacgcgg gcgaacaagt tgcgccggtt cctgcggccc       60

accgttgccc agggcgtgct cggtatcgcg ttctgcctcg tggccgccgt cggcgtggtg      120

cagatccgct cc                                                          132


<210>  12
<211>  111
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  12
atgtcacgga ttcaactccc ccggctgagc cggattgcga tcgcagcagc agcttccgct       60

gccctgatcg gcaccagctt catcgccccg gccacggcct ttgccgcgcc g               111


<210>  13
<211>  75
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  13
ctagccgacc ttctcggcct tgctggccag gtcgtcgggg atcgtgaccc ccatcaggga       60

ggcggccttc tcatt                                                        75


<210>  14
<211>  120
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  14
ttagttgttg gggacgagga gggagtggag ttcgatgacg tcgagggtgg gtgcggtggc       60

ggggcgggtg atggtttcgg tgccggtgtg tccttgggcg gtccaggtga tggtccaggt      120


<210>  15
<211>  60
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  15
tcagccagtt ggtgccggcc ttggcgtcgg cggcgtggga tacacgcgga actgggcgcc       60


<210>  16
<211>  114
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  16
atgttcattt cgcgcttccg tcgtgcggct gcggtcggcc tggccgccgt caccgcattg       60

tccgccactg cctgtagcgg ttcctcgtcg tcgtccagct catccgcgag ctcg            114


<210>  17
<211>  108
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  17
atgaagtccg cgacgcgacg cccgctgacg cgctggattg tcgccttcgg ggtggtgttg       60

gtgctggtca tcgccgggtc ggtggggctg catgcctccg gtgccctg                   108


<210>  18
<211>  108
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  18
gtgaatccct tcgtcaagac ggcgcgcgtg gctatcacct cgacgctggt ggcaggctcg       60

ctcgccactg ccagcctcgt gtttgcacca cttgcacagg ccgattac                   108


<210>  19
<211>  33
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  19

Met Ser Lys Thr Leu Ser Arg Ile Ala Ser Val Ala Ser Val Ala Ala 
1               5                   10                  15      


Leu Ala Gly Ser Ile Thr Val Ile Ala Gly Gln Asn Ala Ser Ala Asp 
            20                  25                  30          


Ser 
    


<210>  20
<211>  30
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  20

Met Lys Asn Gly Leu Lys Thr Leu Leu Ile Gly Gly Val Gly Ile Ala 
1               5                   10                  15      


Thr Leu Ala Val Gly Gly Val Gly Thr Ala Ile Ala Asp Asn 
            20                  25                  30  


<210>  21
<211>  35
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  21

Met Arg Ser Thr Thr Thr Lys Ala Phe Ala Gly Val Ala Val Leu Ala 
1               5                   10                  15      


Leu Ala Leu Ala Gly Cys Gly Ser Asn Ser Gly Ser Ser Thr Lys Ser 
            20                  25                  30          


Ala Asp Ser 
        35  


<210>  22
<211>  31
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  22

Met Leu Thr Arg Lys Arg Val Val Ala Ala Gly Ala Ala Ala Thr Leu 
1               5                   10                  15      


Ser Leu Thr Ala Phe Ala Gly Leu Gln Pro Ala Ser Ala Ala Thr 
            20                  25                  30      


<210>  23
<211>  36
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  23

Met Gly Phe Arg Val Gly Arg Arg Pro Leu Ile Gly Ala Val Leu Ala 
1               5                   10                  15      


Gly Ser Met Ala Thr Leu Val Gly Cys Ser Thr Ser Gly Ser Gly Ser 
            20                  25                  30          


Gly Ala Ser Ser 
        35      


<210>  24
<211>  37
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  24

Met Gln Ala Leu Gln Gly Arg Arg Arg Ser Arg Arg Val Met Ala Ala 
1               5                   10                  15      


Ala Val Ala Ala Leu Thr Ala Met Thr Val Leu Pro Ser Gln Leu Asn 
            20                  25                  30          


Ala Val Ala Ala Pro 
        35          


<210>  25
<211>  30
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  25

Met Ser Thr Gly Arg Met Lys Phe Ile Lys Leu Ala Val Pro Val Ile 
1               5                   10                  15      


Val Ala Cys Cys Leu Thr Pro Met Ala Ala Leu Ala Asp Val 
            20                  25                  30  


<210>  26
<211>  38
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  26

Met Ala Met Arg Ala Arg His Gly Val Val Arg Leu Gly Leu Val Cys 
1               5                   10                  15      


Leu Thr Ala Leu Ala Val Phe Gly Thr Ala Asn Val Ser Gly Gln Val 
            20                  25                  30          


Ala Val Met Ala Glu Gly 
        35              


<210>  27
<211>  36
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  27

Met Asn Gln Ala Leu Ser Thr Met Arg Leu Lys Ile Gly Asp Ser Thr 
1               5                   10                  15      


Lys Arg Ile Arg Ile Phe Phe Val Val Met Ala Val Ala Ile Thr Leu 
            20                  25                  30          


Leu Ala Gly Arg 
        35      


<210>  28
<211>  25
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  28

Met Arg Arg Arg Thr Thr Ile Ala Ala Leu Ala Ala Val Leu Ser Phe 
1               5                   10                  15      


Ser Pro Leu Ala Ala Gln Ala Ala Pro 
            20                  25  


<210>  29
<211>  44
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  29

Met Pro Ser His Ala Val Arg Glu Thr Arg Ala Asn Lys Leu Arg Arg 
1               5                   10                  15      


Phe Leu Arg Pro Thr Val Ala Gln Gly Val Leu Gly Ile Ala Phe Cys 
            20                  25                  30          


Leu Val Ala Ala Val Gly Val Val Gln Ile Arg Ser 
        35                  40                  


<210>  30
<211>  37
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  30

Met Ser Arg Ile Gln Leu Pro Arg Leu Ser Arg Ile Ala Ile Ala Ala 
1               5                   10                  15      


Ala Ala Ser Ala Ala Leu Ile Gly Thr Ser Phe Ile Ala Pro Ala Thr 
            20                  25                  30          


Ala Phe Ala Ala Pro 
        35          


<210>  31
<211>  25
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  31

Met Lys Arg Arg Thr Leu Leu Gly Thr Leu Gly Ile Met Gly Leu Ser 
1               5                   10                  15      


Val Pro Leu Ala Ala Cys Ser Ser Lys 
            20                  25  


<210>  32
<211>  40
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  32

Met Val Thr Gly Gly Asn Asp Met Pro Ser Lys Arg Ile Thr Thr Trp 
1               5                   10                  15      


Pro Gly Ile Ser Ala Leu Ser Ala Leu Ile Ala Gly Met Leu Leu Ala 
            20                  25                  30          


Pro Leu Pro Val Ala Ala Asp Gly 
        35                  40  


<210>  33
<211>  20
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  33

Met Ala Met Val Met Ala Ser Leu Ala Met Phe Gly Ala Ser Arg Ala 
1               5                   10                  15      


Ser Ala Ala Asp 
            20  


<210>  34
<211>  38
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  34

Met Phe Ile Ser Arg Phe Arg Arg Ala Ala Ala Val Gly Leu Ala Ala 
1               5                   10                  15      


Val Thr Ala Leu Ser Ala Thr Ala Cys Ser Gly Ser Ser Ser Ser Ser 
            20                  25                  30          


Ser Ser Ser Ala Ser Ser 
        35              


<210>  35
<211>  36
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  35

Met Lys Ser Ala Thr Arg Arg Pro Leu Thr Arg Trp Ile Val Ala Phe 
1               5                   10                  15      


Gly Val Val Leu Val Leu Val Ile Ala Gly Ser Val Gly Leu His Ala 
            20                  25                  30          


Ser Gly Ala Leu 
        35      


<210>  36
<211>  36
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  36

Met Asn Pro Phe Val Lys Thr Ala Arg Val Ala Ile Thr Ser Thr Leu 
1               5                   10                  15      


Val Ala Gly Ser Leu Ala Thr Ala Ser Leu Val Phe Ala Pro Leu Ala 
            20                  25                  30          


Gln Ala Asp Tyr 
        35      


<210>  37
<211>  572
<212>  PRT
<213>  Propionibacterium freudenreichii

<400>  37

Met Asn Pro Phe Val Lys Thr Ala Arg Val Ala Ile Thr Ser Thr Leu 
1               5                   10                  15      


Val Ala Gly Ser Leu Ala Thr Ala Ser Leu Val Phe Ala Pro Leu Ala 
            20                  25                  30          


Gln Ala Asp Tyr Ser Pro Leu Ala Ala Thr Ala Thr Val Asn Val Arg 
        35                  40                  45              


Gln Gly Pro Asp Thr Ser Ser Ser Val Leu Ala Thr Leu Ser Ser Gly 
    50                  55                  60                  


Asp Thr Val Thr Gln Arg Gly Ala Glu Gln Asp Gly Trp Leu Pro Ile 
65                  70                  75                  80  


Thr Tyr Asn Gly Ala Asn Ala Trp Ile Gln Ala Gln Tyr Val Ala Ser 
                85                  90                  95      


Thr Thr Ala Ala Thr Gln Lys Asp Gln Ile Ser Thr Ala Glu Leu Thr 
            100                 105                 110         


Ala Asp Ala Tyr Val Arg Thr Ala Ala Asn Ala Asn Ala Trp Val Leu 
        115                 120                 125             


Gly Thr Ala His Thr Gly Asp Lys Val Gly Ile Thr Gly Gln Ala Ser 
    130                 135                 140                 


Gly Asp Tyr Thr Pro Val Asn Phe Tyr Gly Arg Ala Gly Trp Ile Ala 
145                 150                 155                 160 


Thr Lys Leu Leu Ser Ala Ala Asp Ala Ser Val Thr Ser Ile Lys Ile 
                165                 170                 175     


Thr Thr Ala Ile Ser Ser Asp Tyr Leu Trp Val Arg Gly Gly Glu Ser 
            180                 185                 190         


Thr Ala Ala Gln Ser Ile Gly Met Leu Tyr Pro Gly Asp Arg Val Asp 
        195                 200                 205             


Val Thr Gly Asp Pro Val Gly Gly Trp Val Pro Ile Asn Phe Asn Gly 
    210                 215                 220                 


Lys Thr Ala Phe Val Ala Ala Asn Tyr Ser Arg Tyr Leu Thr Asp Pro 
225                 230                 235                 240 


Thr Val Val Thr Leu Ser Thr Lys Thr Asp Val Thr Asn Lys Asp Thr 
                245                 250                 255     


Ala Thr Ser Thr Gly Thr Asp Ser Ser Thr Ala Gly Gly Ser Thr Ala 
            260                 265                 270         


Thr Thr Pro Thr Thr Thr Ala Pro Thr Thr Thr Ala Pro Ala Thr Lys 
        275                 280                 285             


Pro Thr Thr Thr Pro Pro Ala Thr Thr Gln Ala Ala Ala Ser Thr Lys 
    290                 295                 300                 


Tyr Thr Thr Ala Asp Val Asn Val Arg Val Gly Pro Gly Ile Asp Gln 
305                 310                 315                 320 


Gln Pro Val Thr Val Leu Lys Glu Asn Ser Gln Val Ala Ala Thr Gly 
                325                 330                 335     


Lys Thr Ser Gly Asp Trp Thr Glu Val Ser Tyr Asp Gly Ala Ser Arg 
            340                 345                 350         


Trp Ile Ser Ser Gln Tyr Leu Ser Asp Thr Lys Gln Ala Glu Ala Pro 
        355                 360                 365             


Ala Pro Ala Pro Ala Pro Asp Pro Thr Pro Ala Gly Pro Thr Gly Ser 
    370                 375                 380                 


Arg Trp Thr Thr Ala Ala Leu Asn Ala Tyr Gly Ser Ser Thr Gln Pro 
385                 390                 395                 400 


Lys Pro Ala Thr Thr Val Val Pro Glu Gly Thr Gln Val Glu Leu Thr 
                405                 410                 415     


Gly Lys Gln Ala Asp Gly Arg Ser Glu Tyr Thr Trp Asn Gly Thr Thr 
            420                 425                 430         


Tyr Trp Ser Ala Thr Glu Tyr Leu Gly Thr Asn Ala Pro Ala Thr Asn 
        435                 440                 445             


Thr Ser Ala Asn Thr Ala Lys Pro Gly Ala Asn Ala Val Glu Thr Ala 
    450                 455                 460                 


Ile Asn Phe Ala Met Ser Lys Leu Gly Gly Pro Tyr Val Trp Gly Gly 
465                 470                 475                 480 


Thr Gly Pro Val Gly Tyr Asp Cys Ser Gly Leu Met Gln Ala Ala Tyr 
                485                 490                 495     


Ala Ala Ala Gly Val Thr Leu Pro Arg Val Thr Trp Asp Gln Val Asn 
            500                 505                 510         


Ala Gly Lys Gln Val Ser Val Gly Asp Leu Gln Pro Gly Asp Leu Val 
        515                 520                 525             


Phe Phe Tyr Asp Asn Gly His Val Gly Met Tyr Ile Gly Asn Gly Asn 
    530                 535                 540                 


Ile Val Asn Ala Leu Asn Glu Asp Ala Gly Ile Val Val Thr Pro Ile 
545                 550                 555                 560 


Ser Tyr Met Pro Ile Ser Ala Ala Val Arg Ile Ala 
                565                 570         


<210>  38
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  Primer P963Fw

<400>  38
atacatatgc caccgtgagc tgcacct                                           27


<210>  39
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  Primer P963Rv

<400>  39
gcaagctttc ggcctgtgca agtggtg                                           27


<210>  40
<211>  37
<212>  DNA
<213>  artificial

<220>
<223>  Primer TRAILFw

<400>  40
gcaagcttag tgagagaaag aggtcctcag agagtag                                37


<210>  41
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  Primer TRAILRv

<400>  41
actgcagtta gccaactaaa aaggccccga aaaaactgg                              39


<210>  42
<211>  9073
<212>  DNA
<213>  artificial

<220>
<223>  Sequence of the plasmid pFB4:TRAIL

<400>  42
ggtcgactct agagggatcc ggcggaactt cacgtcctgg cggtggagtt ggcgggcgcg       60

ttccagccgt tcctccagca cggtgatccg ggcctccaga cgctcacgct caccctgctc      120

caggtgccgg gtcaccgtca ccgtccgcac cggccgggcc tcggcctggg cggcccggcg      180

ttcctcactg gcccgcttcc ggcaatcgtc ggaacaccac acccggggcc gaccccgccc      240

accgtgggcc tccaccggcg ccccgcagtg gggacacgcc cgcagcgccg acgcatcctc      300

atccaaggcc atcaccgggt cggaatccat acccgaaacc atatcgtccg gacgatgaac      360

tgcgccagac agctaagaat gcacgaggtg tgtctccgat tctcaggaaa cgctcagcat      420

tttccgagac gttcggcgca cgcacacacc cccacaagaa ccgacccgcc cagcatccgc      480

cgacacgtcg atccgcaccc gcgatgggct ggccgaggcc gactacgacc gctagtcagc      540

acctgcgctg atctaccgtc gccctgaccg actctcccgt cgggattgtc gccggccgct      600

gccagcatgg acctgcggcc ccgccccctc gccctgcaac tcgagggagg cggggccgtc      660

caccccccac accaccccga caccgtgatg cgcccatgtc gcctaacggg ttgcccgacc      720

tccccgacat caagaaaacc tgacaccgtc gccgcaagcg ctacactgac tactagtagt      780

caggaggtgc gtgatgacca tcgccacatc ggtgaaactc tccgaagaga ccggccgcaa      840

actcgatgaa ctagcccggg ccaccgggcg atccaagtcc tactacctgc gcgaggccat      900

cgaggaccac atcgaccaga tggtccacga ctacgccatc gcccgactcg ccgacgacgt      960

gcgagccggc cgggccgcca cctacagcgc cgacgaagtg gaccagatcc ttggcctgga     1020

cgattgagta caccgacccc gccgtcaaag cactgcgcaa actcgaccga gcccaggccc     1080

gccgcatcac cgcctacata cgtgagctca ccggcctgga cgatccccac caacgcggga     1140

aaggaaaata aaaaagggga cctctagggt ccccaattaa ttagtaatat aatctattaa     1200

aggtcattca aaaggtcatc caccggatca attcccctgc tcgcgcaggc tgggtgccaa     1260

gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt     1320

gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgtaatgt     1380

gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt     1440

gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc     1500

caagctgtgg cgtacaccgt cgcctcggtc ggcccgtaga gattggcgat cccgaccgca     1560

gcaccaccga gaacgtcccc gacgtggccg accagcccgt catcgtcaac gcctgaccgc     1620

ggtgcggaca ggccgtgtcg cgaccggccg tgcggaatta agccggcccg taccctgtga     1680

atagaggtcc gctgtgacac aagaatccct gttacttctc gaccgtattg attcggatga     1740

ttcctacgcg agcctgcgga acgaccagga attctgggag ccgctggccc gccgagccct     1800

ggaggagctc gggctgccgg tgccgccggt gctgcgggtg cccggcgaga gcaccaaccc     1860

cgtactggtc ggcgagcccg acccggtgat caagctgttc ggcgagcact ggtgcggtcc     1920

ggagagcctc gcgtcggagt cggaggcgta cgcggtcctg gcggacgccc cggtgccggt     1980

gccccgcctc ctcggccgcg gcgagctgcg gcccggcacc ggagcctggc cgtggcccta     2040

cctggtgatg agccggatga ccggcaccac ctggcggtcc gcgatggacg gcacgaccga     2100

ccggaacgcg ctgctcgccc tggcccgcga actcggccgg gtgctcggcc ggctgcacag     2160

ggtgccgctg accgggaaca ccgtgctcac cccccattcc gaggtcttcc cggaactgct     2220

gcgggaacgc cgcgcggcga ccgtcgagga ccaccgcggg tggggctacc tctcgccccg     2280

gctgctggac cgcctggagg actggctgcc ggacgtggac acgctgctgg ccggccgcga     2340

accccggttc gtccacggcg acctgcacgg gaccaacatc ttcgtggacc tggccgcgac     2400

cgaggtcacc gggatcgtcg acttcaccga cgtctatgcg ggagactccc gctacagcct     2460

ggtgcaactg catctcaacg ccttccgggg cgaccgcgag atcctggccg cgctgctcga     2520

cggggcgcag tggaagcgga ccgaggactt cgcccgcgaa ctgctcgcct tcaccttcct     2580

gcacgacttc gaggtgttcg aggagacccc gctggatctc tccggcttca ccgatccgga     2640

ggaactggcg cagttcctct gggggccgcc ggacaccgcc cccggcgcct gacgccccgg     2700

gcctaccgct gacacgcaac cccgcaccct cggccaagac gtcacacacc acccgcccca     2760

ccgagcactg aggatgtcaa ctcgcccgag ccggcctgcc ggccgtctta cgggttgtct     2820

tggcgggcgg ggtgtctttg ccctggccca gcagccccac gatctcccgc agcgtgtcgg     2880

cggtggcggc gtcccgggcc gcctgacgct ccgcctccgc cctggcctgc tcggctgcct     2940

gcgcccgatc ctccgcggcg gcggcctgct ccctcgcctc ggccagctcg ccggtcaggg     3000

cctcgacccg ggcctgcacc tgccccaggc gcgcctccgc ctcctgctgc acctgctcgg     3060

cccgggcctc cgcctggtcc cgggccgcct cggcctcggc ccggtgctga tccgccaggg     3120

ccgcctcggc caccgcttcg gcctgcccat ccaccgcctg ctcggcccga gccccgaact     3180

cctcgcgggc cgcatcactc gcctgacgcc acgccgccgc ccacaccaga cccaacggct     3240

ccgacagatc cggcggggcc ggcgtctgga ccgacgccga gacgtcgcgc aggaaccccg     3300

ccgcagcgtc ggtggagcac cccgcctccg ccttcaacga ccgcaccgtc acccgccgac     3360

ccgcaccgct caaccgcgca taggccgccg ccaaccttga cccattcgac tccatgaccc     3420

accctcccat tctgtaccct gtacctgttc ctaggtacgt tcctaatgta cctcaccgga     3480

tgcagaaccc gcaacccccc tcacactccc cctgcacggg gcccgccccc tgcacccccg     3540

ctgccgcgcc cgctcctgcg tcgcggcctt gcccctgccc aacgccgggc cggcgggcag     3600

cccaccagag gctctgtgag acgtcggcgc ccccgtccac ctaccctaaa gaccaaccgg     3660

ccgtggaaac gtctgtgagg agccttgtag gagttcccag gacaagccag caaggccggg     3720

cctgacggcc cggaaaggaa gtcgctgcgc tcctacgaag aagcccctct ggggaccccc     3780

agaccccgga actatctgat ttggtttagc ggcgtacttc cgtcataccg gaatttatgg     3840

catgctgtgg tcatggcgac gacgacggtc gatgagcagt gggagcaggt gtggctgccc     3900

cgctggcccc tggcctccga cgacctggca gcgggcatct accggatggc ccgcccctcg     3960

gcgctggggg tccgatacat cgaggtcaac ccccaagcca tcagcaacct cctcgtggtc     4020

gactgcgacc accccgacgc tgccatgcgc gccgtctggg accgccacga ctggctgccc     4080

aacgccatcg tcgagaaccc cgacaacggc cacgcccacg ccgtgtgggc cctggaagca     4140

gccatcccgc gcaccgagta cgcccaccgc aagcccatcg cctacgccgc cgccgtcacc     4200

gagggcctgc gccgatccgt cgacggagac gcctcctacg ccggcctgat caccaagaac     4260

cccgaacacc ccgcctggaa caccacctgg tgcaccgacc acctctaccg gctggccgag     4320

ctcgacaccc acctggatgc cgccggcctc atgcccgccc cctcctggcg acgcacccgc     4380

cggcgcaacc ccgtcggcct gggccgcaac tgcgccatct tcgagaccgc ccgcacctgg     4440

gcctaccgcg acgcccgccg catccgacaa cgccacgaat acccgaccgc cgaggactcg     4500

gccgacctgc acgccgtcat cgcctccacc gtcgaggcgc tcaacgccgg ctacagcgaa     4560

cccctgccgg cccgcgaggc cgccggcatc gccgccagca tccaccgatg gatcacccac     4620

cgtttctacg gctggatcga ctcccacacc gtcaacgagg ccactttctc caccatccag     4680

agctacagag gacacaaggg agccggcaag gctcgtcctc gtgcccgccg tgctgcttct     4740

atcaccgatt gggaggcatg atggctgacg tccagcaccg cgtgaagcgt cggggcacgg     4800

cccgcgaggc cgcagaacgt gtaggggcct ccatccgaac cgcccagcgg tggacctcca     4860

tcccccgtga ggaatggatc actcagaagg ccgtcgagcg tgaggagatc cgggcctaca     4920

agtacgacga ggggcacacg tggggcgaga cctcgcgcca cttcgggatc gcgaagacca     4980

ccgcccagga gcgggcccgg cgggctcgaa gggagcgggc ggccgaagcg gagaaggctg     5040

ccgaggaggc cgaggccgcg ctgcgtccga cactcttcga gggccaggag caaggttctg     5100

catgagcaac cccgagtcct cgggtagacc gtctggcccg acgttaagca tggctgaagc     5160

ggcccgtgcc tgtggggttt cagtgtccac ggtgaggcgt caccgtgatg ccctggtggc     5220

ccacggtgct acccgtcatg acgcgtcatg ggtgataccc ctatcagcgt tgatttcatg     5280

cggtttgatg ccccgggtga caccccctga tgccccgtca cccaataacg tggcgcctgc     5340

catgacgtcc cacggtgacg cccccctgac gggggaagtc caagagctgc gcgagcgact     5400

ggccaacgct gagcatcgag ccgagctagc agtagaggtt ggggacgacg tctcggcgac     5460

tccggagaac accaagtcag ggtctcatga gtgtgcgata gcttgagctg tctaccaatc     5520

tggatatagc tatatcggtc gtttgtgtct gattcgccag tgagccaacg gcgggggcga     5580

cacgcggtgg cgaaaccccc tggcagaatt cgtaatcatg gtcatagctg tttcctgtgt     5640

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag     5700

cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt     5760

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag     5820

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     5880

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     5940

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     6000

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     6060

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     6120

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     6180

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     6240

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     6300

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     6360

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     6420

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     6480

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     6540

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     6600

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     6660

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     6720

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     6780

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     6840

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     6900

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     6960

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     7020

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     7080

acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat     7140

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag     7200

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac     7260

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt     7320

ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt     7380

gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc     7440

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat     7500

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca     7560

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga     7620

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg     7680

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg     7740

ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga     7800

cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg     7860

acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg     7920

atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct     7980

ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg ccaccgtgag     8040

ctgcacctga atcagctgag aatgccctga accctcaaac ccgcaccctg aacctcaacc     8100

ttgcgttgaa cccgaggggt gcgggttgga cgcgcacccc gaccactgca ccccgcgggg     8160

cgcccctgtg acgaccatgg tctgattcac gcctgaaatc actccccgcc ggggtggaga     8220

accacgtcaa cgcggccgtg gatcacatcg ggcgtcgaaa aacaaccccc catttcccca     8280

accctcaacc tgatcctgca ctgttgtcgg gtttgctgag agccgcctaa gctgccgcac     8340

gttgtcccag ttggggcgtg gcctgctgca tacggggccg ggaaagacgc ctcacctggg     8400

atgacgcgga ccattggaca cggcctttcc ggccgcggga aggaccagac gtgaatccct     8460

tcgtcaagac ggcgcgcgtg gctatcacct cgacgctggt ggcaggctcg ctcgccactg     8520

ccagcctcgt gtttgcacca cttgcacagg ccgaaagctt agtgagagaa agaggtcctc     8580

agagagtagc agctcacata actgggacca gaggaagaag caacacattg tcttctccaa     8640

actccaagaa tgaaaaggct ctgggccgca aaataaactc ctgggaatca tcaaggagtg     8700

ggcattcatt cctgagcaac ttgcacttga ggaatggtga actggtcatc catgaaaaag     8760

ggttttacta catctattcc caaacatact ttcgatttca ggaggaaata aaagaaaaca     8820

caaagaacga caaacaaatg gtccaatata tttacaaata cacaagttat cctgacccta     8880

tattgttgat gaaaagtgct agaaatagtt gttggtctaa agatgcagaa tatggactct     8940

attccatcta tcaaggggga atatttgagc ttaaggaaaa tgacagaatt tttgtttctg     9000

taacaaatga gcacttgata gacatggacc atgaagccag ttttttcggg gcctttttag     9060

ttggctaact gca                                                        9073


<210>  43
<211>  205
<212>  PRT
<213>  artificial

<220>
<223>  Fusion protein sequence encoded by pFB4:TRAIL

<400>  43

Met Asn Pro Phe Val Lys Thr Ala Arg Val Ala Ile Thr Ser Thr Leu 
1               5                   10                  15      


Val Ala Gly Ser Leu Ala Thr Ala Ser Leu Val Phe Ala Pro Leu Ala 
            20                  25                  30          


Gln Ala Glu Ser Leu Val Arg Glu Arg Gly Pro Gln Arg Val Ala Ala 
        35                  40                  45              


His Ile Thr Gly Thr Arg Gly Arg Ser Asn Thr Leu Ser Ser Pro Asn 
    50                  55                  60                  


Ser Lys Asn Glu Lys Ala Leu Gly Arg Lys Ile Asn Ser Trp Glu Ser 
65                  70                  75                  80  


Ser Arg Ser Gly His Ser Phe Leu Ser Asn Leu His Leu Arg Asn Gly 
                85                  90                  95      


Glu Leu Val Ile His Glu Lys Gly Phe Tyr Tyr Ile Tyr Ser Gln Thr 
            100                 105                 110         


Tyr Phe Arg Phe Gln Glu Glu Ile Lys Glu Asn Thr Lys Asn Asp Lys 
        115                 120                 125             


Gln Met Val Gln Tyr Ile Tyr Lys Tyr Thr Ser Tyr Pro Asp Pro Ile 
    130                 135                 140                 


Leu Leu Met Lys Ser Ala Arg Asn Ser Cys Trp Ser Lys Asp Ala Glu 
145                 150                 155                 160 


Tyr Gly Leu Tyr Ser Ile Tyr Gln Gly Gly Ile Phe Glu Leu Lys Glu 
                165                 170                 175     


Asn Asp Arg Ile Phe Val Ser Val Thr Asn Glu His Leu Ile Asp Met 
            180                 185                 190         


Asp His Glu Ala Ser Phe Phe Gly Ala Phe Leu Val Gly 
        195                 200                 205 


<210>  44
<211>  1719
<212>  DNA
<213>  Propionibacterium freudenreichii

<400>  44
gtgaatccct tcgtcaagac ggcgcgcgtg gctatcacct cgacgctggt ggcaggctcg       60

ctcgccactg ccagcctcgt gtttgcacca cttgcacagg ccgattactc cccccttgcg      120

gccaccgcca cggtgaatgt gcgccagggc cccgacacct ccagctcggt gctggcgacg      180

ctctcgtcgg gtgacacggt cacccagcgg ggagccgaac aggacggctg gctgccgatc      240

acctacaacg gcgccaacgc gtggatccag gcgcagtacg tggcctccac cacggccgcc      300

acccagaagg accagatctc caccgccgag ctcacggccg atgcctatgt gcgcaccgcg      360

gccaatgcga acgcctgggt gttgggcacg gcccacaccg gcgacaaggt gggcatcacc      420

ggccaggcca gcggcgacta cacgccggtg aacttctacg gccgcgccgg ttggatcgcc      480

accaagctgc tcagcgcggc cgacgcctcg gtgacatcga tcaagatcac caccgccatc      540

tcgagcgact acctgtgggt gcgtggtggc gagagcaccg cggcccagtc catcggcatg      600

ctctacccgg gtgaccgggt ggacgtgacc ggcgatcccg tcggcgggtg ggtgccgatc      660

aacttcaacg gcaagaccgc cttcgtggcc gcgaactact cgcgctatct cactgatccc      720

accgtggtca cgctgtcgac gaagaccgac gtcaccaaca aggacacggc cacctcgacg      780

ggcaccgatt cctcgacggc cggcggctcg accgccacca ccccgaccac cacggcgcca      840

accaccaccg ctcctgcaac caagccgacg accacgcccc cggccacgac gcaggccgct      900

gcgtccacga agtacacgac ggccgacgtc aacgtgcgcg tgggacccgg catcgaccag      960

cagccggtga cggtgctcaa ggagaactcg caggtggccg ccaccggcaa gacaagtggc     1020

gactggaccg aggtcagcta cgacggcgcc tcgcgctgga tcagcagcca gtacctctcg     1080

gacaccaagc aggccgaggc acccgccccg gcacctgcgc ccgatccgac gcccgccggc     1140

cccaccggaa gccggtggac cacggcggca ctgaacgcct atggcagctc cacccagccc     1200

aagccggcca ccacggtggt gcccgagggc acccaggtgg aactgaccgg caagcaggcc     1260

gatggacgct cggagtacac gtggaacggc acgacctatt ggtcggccac cgaatacctc     1320

ggcaccaatg cgccggccac gaacacctca gcgaacaccg ccaagccggg cgccaacgcg     1380

gtggagacgg cgatcaactt cgcgatgtcg aagctcggtg gcccctatgt ctggggcggc     1440

accggtccgg tgggctatga ctgctccgga ctgatgcagg ccgcgtacgc ggcggccggc     1500

gtcaccctgc cgcgcgtcac ctgggaccag gtgaatgccg gcaagcaggt gtcggtcggc     1560

gacctgcagc cgggcgacct ggtgttcttc tatgacaacg gccacgtggg catgtacatc     1620

ggcaacggca acatcgtcaa cgccctcaac gaggacgccg gcatcgtggt gaccccgatc     1680

agctatatgc cgatctcggc tgccgtccgg atcgcctga                            1719


<210>  45
<211>  26
<212>  PRT
<213>  Propionibacterium acnes

<400>  45

Met Ser Lys Val Val Ala Ser Ala Ile Ala Gly Ala Leu Ser Leu Thr 
1               5                   10                  15      


Ser Ala Gly Gly Leu Thr Met Val Gln Ala 
            20                  25      


<210>  46
<211>  30
<212>  PRT
<213>  Propionibacterium acnes

<400>  46

Met Arg Lys Ala Ile Val Thr Pro Val Ala Val Leu Ala Val Leu Val 
1               5                   10                  15      


Met Ala Leu Thr Gly Cys Gly Gln Lys Asn Gln Ser Gly Gly 
            20                  25                  30  


<210>  47
<211>  29
<212>  PRT
<213>  Propionibacterium acnes

<400>  47

Met Ala Ser Thr Pro Arg Arg Arg Trp Ala Trp Val Leu Leu Leu Val 
1               5                   10                  15      


Val Ala Ser Leu Val Ile Val Gly Val Tyr Arg Lys Ala 
            20                  25                  


<210>  48
<211>  26
<212>  PRT
<213>  Propionibacterium acnes

<400>  48

Met Ser Ser Met Lys Gly Leu Ser Leu Val Leu Ala Thr Ser Phe Met 
1               5                   10                  15      


Leu Ser Phe Ser Pro Gly Ser Ser Phe Ala 
            20                  25      


<210>  49
<211>  44
<212>  PRT
<213>  Propionibacterium acnes

<400>  49

Met Glu His Arg Tyr Gly Ala Ser Gln Val Ser Gly Ser Ala Pro Arg 
1               5                   10                  15      


Arg Gly Arg Gly Val Ala Phe Ala Ala Ile Thr Gly Ala Ile Leu Leu 
            20                  25                  30          


Gly Thr Val Ala Ser Val Asp Pro Gly Ala Gln Ala 
        35                  40                  


<210>  50
<211>  27
<212>  PRT
<213>  Propionibacterium acnes

<400>  50

Met Ser Ser Met Lys Gly Leu Ser Leu Val Leu Ala Thr Ser Phe Met 
1               5                   10                  15      


Leu Ser Phe Ser Pro Gly Ser Ser Phe Ala Ser 
            20                  25          


<210>  51
<211>  47
<212>  PRT
<213>  Propionibacterium acnes

<400>  51

Met Pro His Ser Asp Gln Pro Thr Ser Lys Arg Val Met Ser Ala Pro 
1               5                   10                  15      


Arg Arg Arg Met Pro Gly Trp Val Pro Val Thr Val Gly Ile Ala Val 
            20                  25                  30          


Val Val Ile Val Val Val Ala Val Ile Val Ser Ser Leu Arg Ser 
        35                  40                  45          


<210>  52
<211>  32
<212>  PRT
<213>  Propionibacterium acnes

<400>  52

Met Phe Gly Thr Pro Ser Arg Arg Thr Phe Leu Thr Ala Ser Ala Leu 
1               5                   10                  15      


Ser Ala Met Ala Leu Ala Ala Ser Pro Thr Val Thr Asp Ala Ile Ala 
            20                  25                  30          


<210>  53
<211>  26
<212>  PRT
<213>  Propionibacterium acnes

<400>  53

Met Lys Ile Asn Ala Arg Phe Ala Val Met Ala Ala Ser Val Ala Val 
1               5                   10                  15      


Leu Met Ala Ala Ala Pro Ile Ala Gln Ala 
            20                  25      


<210>  54
<211>  50
<212>  PRT
<213>  Propionibacterium acnes

<400>  54

Met Tyr Pro Val His Leu Pro Leu Arg Asn Glu Ser Glu Phe Ser Phe 
1               5                   10                  15      


Arg Ala His Asn His Gly Gly Thr Val Pro Ser Arg Leu Thr Arg Arg 
            20                  25                  30          


Ser Val Leu Ala Thr Gly Ala Val Ala Leu Pro Met Thr Ala Ala Ala 
        35                  40                  45              


Cys Ala 
    50  


<210>  55
<211>  27
<212>  PRT
<213>  Propionibacterium acnes

<400>  55

Met Arg His Met Arg Pro Leu Ile Ala Leu Ser Leu Ala Gly Leu Met 
1               5                   10                  15      


Thr Leu Ser Ala Cys Gly Glu Asp Val Ala Ala 
            20                  25          


<210>  56
<211>  31
<212>  PRT
<213>  Propionibacterium acnes

<400>  56

Met Asn Arg Thr Leu Lys Val Ala Ala Val Gly Ala Ile Ala Ile Leu 
1               5                   10                  15      


Cys Leu Ala Ala Cys Ser Asp Pro Gly Ser Asp Ser Ala Gln Ser 
            20                  25                  30      


<210>  57
<211>  24
<212>  PRT
<213>  Propionibacterium acnes

<400>  57

Met Glu Lys Ser Ser Phe Ala Ala Ala Asn Met Thr Ile Met Ser Glu 
1               5                   10                  15      


Pro Thr Thr Pro Thr Ser Gln Ala 
            20                  


<210>  58
<211>  281
<212>  PRT
<213>  Homo sapiens

<400>  58

Met Ala Met Met Glu Val Gln Gly Gly Pro Ser Leu Gly Gln Thr Cys 
1               5                   10                  15      


Val Leu Ile Val Ile Phe Thr Val Leu Leu Gln Ser Leu Cys Val Ala 
            20                  25                  30          


Val Thr Tyr Val Tyr Phe Thr Asn Glu Leu Lys Gln Met Gln Asp Lys 
        35                  40                  45              


Tyr Ser Lys Ser Gly Ile Ala Cys Phe Leu Lys Glu Asp Asp Ser Tyr 
    50                  55                  60                  


Trp Asp Pro Asn Asp Glu Glu Ser Met Asn Ser Pro Cys Trp Gln Val 
65                  70                  75                  80  


Lys Trp Gln Leu Arg Gln Leu Val Arg Lys Met Ile Leu Arg Thr Ser 
                85                  90                  95      


Glu Glu Thr Ile Ser Thr Val Gln Glu Lys Gln Gln Asn Ile Ser Pro 
            100                 105                 110         


Leu Val Arg Glu Arg Gly Pro Gln Arg Val Ala Ala His Ile Thr Gly 
        115                 120                 125             


Thr Arg Gly Arg Ser Asn Thr Leu Ser Ser Pro Asn Ser Lys Asn Glu 
    130                 135                 140                 


Lys Ala Leu Gly Arg Lys Ile Asn Ser Trp Glu Ser Ser Arg Ser Gly 
145                 150                 155                 160 


His Ser Phe Leu Ser Asn Leu His Leu Arg Asn Gly Glu Leu Val Ile 
                165                 170                 175     


His Glu Lys Gly Phe Tyr Tyr Ile Tyr Ser Gln Thr Tyr Phe Arg Phe 
            180                 185                 190         


Gln Glu Glu Ile Lys Glu Asn Thr Lys Asn Asp Lys Gln Met Val Gln 
        195                 200                 205             


Tyr Ile Tyr Lys Tyr Thr Ser Tyr Pro Asp Pro Ile Leu Leu Met Lys 
    210                 215                 220                 


Ser Ala Arg Asn Ser Cys Trp Ser Lys Asp Ala Glu Tyr Gly Leu Tyr 
225                 230                 235                 240 


Ser Ile Tyr Gln Gly Gly Ile Phe Glu Leu Lys Glu Asn Asp Arg Ile 
                245                 250                 255     


Phe Val Ser Val Thr Asn Glu His Leu Ile Asp Met Asp His Glu Ala 
            260                 265                 270         


Ser Phe Phe Gly Ala Phe Leu Val Gly 
        275                 280     


<210>  59
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein A of Propionibacterium 
       freudenreichii strain CIRM BIA N 118

<400>  59

Met Ala Thr Gly Ala Ala Ala Ala Met Phe Val Thr Thr Phe Ala Gly 
1               5                   10                  15      


Met Ala Pro Ala Asn Ala 
            20          


<210>  60
<211>  29
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein B of Propionibacterium 
       freudenreichii strain CIRM BIA N 118

<400>  60

Met Ser Val Arg Lys Ser Leu Thr Gly Met Ala Leu Gly Leu Ala Leu 
1               5                   10                  15      


Thr Ile Thr Pro Leu Ala Gly Ala Val Pro Ala Ser Ala 
            20                  25                  


<210>  61
<211>  28
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein D of Propionibacterium 
       freudenreichii strain CIRM BIA N 118

<400>  61

Met Arg Arg Phe Phe Ser Ala Ala Ile Ala Ile Leu Leu Ala Ala Thr 
1               5                   10                  15      


Leu Thr Pro Ala Leu Asn Ala Pro Met Ala Ser Ala 
            20                  25              


<210>  62
<211>  28
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein D of Propionibacterium 
       freudenreichii strain CIRM BIA N 121

<400>  62

Met Arg Arg Phe Phe Ser Ala Ala Ile Ala Ile Leu Leu Ala Ala Thr 
1               5                   10                  15      


Leu Thr Pro Ala Leu Asn Ala Pro Met Ala Ser Ala 
            20                  25              


<210>  63
<211>  29
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein B of Propionibacterium 
       freudenreichii strain CIRM BIA N 122

<400>  63

Met Ser Val Arg Lys Ser Leu Thr Gly Met Ala Leu Gly Leu Ala Leu 
1               5                   10                  15      


Thr Ile Thr Pro Leu Ala Gly Ala Val Pro Ala Ala Ala 
            20                  25                  


<210>  64
<211>  28
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein D of Propionibacterium 
       freudenreichii strain CIRM BIA N 122

<400>  64

Met Arg Arg Phe Phe Ser Ala Ala Ile Ala Ile Leu Leu Ala Ala Thr 
1               5                   10                  15      


Leu Thr Pro Ala Leu Asn Ala Pro Met Ala Ser Ala 
            20                  25              


<210>  65
<211>  33
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein E of Propionibacterium 
       freudenreichii strain CIRM BIA N 122

<400>  65

Met Lys Thr Arg Val Arg Ser Arg Lys Pro Ala Ala Gly Leu Ala Gly 
1               5                   10                  15      


Ile Ala Leu Phe Ala Ser Gly Leu Ser Leu Met Ser Thr Val Ala Ser 
            20                  25                  30          


Arg 
    


<210>  66
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein A of Propionibacterium 
       freudenreichii strain CIRM BIA N 125

<400>  66

Met Ala Thr Gly Ala Ala Ala Ala Met Phe Val Thr Thr Phe Ala Gly 
1               5                   10                  15      


Met Ala Pro Ala Asn Ala 
            20          


<210>  67
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein A of Propionibacterium 
       freudenreichii strain CIRM BIA N 129

<400>  67

Met Ala Thr Gly Ala Ala Ala Ala Met Phe Val Thr Thr Phe Ala Gly 
1               5                   10                  15      


Met Ala Pro Ala Asn Ala 
            20          


<210>  68
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  Signal peptide of Surface layer protein A of Propionibacterium 
       freudenreichii strain CIRM BIA N 512

<400>  68

Met Ala Thr Gly Ala Ala Ala Ala Met Phe Val Thr Thr Phe Ala Gly 
1               5                   10                  15      


Met Ala Pro Ala Asn Ala 
            20          


<210>  69
<211>  624
<212>  DNA
<213>  artificial

<220>
<223>  DNA fragment from Propionibacterium freudenreichii strain CIRM 
       BIA N 118 comprising the promotor and the signal peptide of the 
       Surface layer protein A

<400>  69
gatcccccga ggtccacacg ccgggccgag gcgggtacac ctgccgggca ggccgggggt       60

tccggctctg ggaatggagc tgttcggtta tccaccatgt ggacgacacg caggcaccat      120

gctcgtcttc gagcggaatc acgggccccg ataactgtgt gatttgtgcg tgaaggccga      180

ttcgggggcc gtttgagcgg cgcgccgcac ggataacagc gttctatgcc accgatacac      240

caatagtccc caagtgcttg acaggactct tcgaagcctc tttgaagatg gctctgacta      300

ggggaaacag tgctcagtag tcaccgcgaa ggagcagtgg ctctgttact gttctagcgg      360

ctggacgggt agttcgattg atgatacgct tgcctcggca tagtgggtcg ccaatcctag      420

ggaacccatc gaaggccatg aattttccca gaggacatgg gtgttcgaac atcccggcag      480

agctcttgaa ggtcggccgt gcacccttgt ccaggcttga aaggaaacaa gttgtctgtt      540

cgtaggattg cggtggccac aggtgctgcg gccgccatgt ttgtgacgac gtttgcgggc      600

atggcgcctg cgaatgccaa ggat                                             624


<210>  70
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  Forward primer PSlpA_fwd_BamHI

<400>  70
atgcggatcc ccccgaggtc cacacgccgg                                        30


<210>  71
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  Reverse primer PS_SlpA-EcoRV-Rev_bis

<400>  71
atgcgatatc ggcattcgca ggcgccatgc                                        30


