                         SEQUENCE LISTING

<110>  Larix Bioscience LLC
 
<120>  Methods and Compositions for Producing Double Allele Knock Outs

<130>  LRX.0004

<150>  US 61/856,579
<151>  2013-07-19

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  203
<212>  DNA
<213>  Cricetulus griseus

<400>  1
atgcgggcat ggactggttc ctggcgttgg attatgctca ttctttttgc ctgggggacc       60

ttattgtttt atataggtgg tcatttggtt cgagataatg accaccctga ccattctagc      120

agagaactct ccaagattct tgcaaagctg gagcgcttaa aacaacaaaa tgaagacttg      180

aggagaatgg ctgagtctct ccg                                              203


<210>  2
<211>  205
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  alpha 1,6 fucosyltransferase exon 1 mutant

<400>  2
atgcgggcat ggactggttc ctggcgttgg attatgctca ttctttttgc ctgggggacc       60

ttattgtttt atataggtgg tcatttggtt cgagataatg accaccctga ccccattcta      120

gcagagaact ctccaagatt cttgcaaagc tggagcgctt aaaacaacaa aatgaagact      180

tgaggagaat ggctgagtct ctccg                                            205


<210>  3
<211>  202
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  alpha 1,6 fucosyltransferase exon 1 mutant

<400>  3
atgcgggcat ggactggttc ctggcgttgg attatgctca ttctttttgc ctgggggacc       60

ttattgtttt atataggtgg tcatttggtt cgagataatg accaccctgc cattctagca      120

gagaactctc caagattctt gcaaagctgg agcgcttaaa acaacaaaat gaagacttga      180

ggagaatggc tgagtctctc cg                                               202


<210>  4
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  alpha 1,6 fucosyltransferase exon 1 mutant

<400>  4
atgcgggcat ggactggtcc attctagcag agaactctcc aagattcttg caaagctgga       60

gcgcttaaaa caacaaaatg aagacttgag gagaatggct gagtctctcc g               111


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  5
gcatggactg gttcctggcg ttgg                                              24


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  6
gttctctgct agaatggtca ggg                                               23


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  7
gctccagctt tgcaagaatc ttgg                                              24


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  8
ggagaatggc tgagtctctc cgg                                               23


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  9
gaataccaga aggccctatt gatcagg                                           27


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  10
gaagaaatta aagaaattag aagg                                              24


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  11
gaatttcatc tgcatgtctt tgg                                               23


<210>  12
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  12
gatctatact acctcagtca aacagatgg                                         29


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  13
gaagccaaag atctgacaga gctgg                                             25


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  14
gcagatatgt tattctccgc tgg                                               23


<210>  15
<211>  90
<212>  DNA
<213>  Streptococcus pyogenes

<400>  15
atcttgttgg aaccattcaa aacagcatag caagttaaaa taaggctagt ccgttatcaa       60

cttgaaaaag tggcaccgag tcggtgcttt                                        90


<210>  16
<211>  264
<212>  DNA
<213>  Homo sapiens

<400>  16
aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac       60

aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa      120

aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt      180

aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat      240

atcttgtgga aaggacgaaa cacc                                             264


<210>  17
<211>  4116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence encoding Cas9 Protein

<400>  17
atggacaaga aatattcaat cggacttgac attggaacaa actctgtcgg ctgggccgtc       60

attactgatg agtacaaagt gccatccaag aagttcaaag tattgggcaa cacagatcgg      120

cacagtatca aaaaaaacct gatcggcgca ctgctgtttg actctggaga aaccgctgag      180

gctactagac ttaagcgcac tgcccggcgt aggtacacca ggagaaagaa tcgcatctgt      240

tatctgcagg agatctttag caacgaaatg gctaaggttg acgatagttt tttccaccgc      300

ctggaggaga gtttcctcgt agaggaggac aagaaacacg agcgtcaccc tattttcgga      360

aacatcgtgg atgaagtcgc ttaccacgag aagtatccta caatttatca cttgcgcaag      420

aaactcgtgg atagcacaga caaagctgac ctgcgcctta tctatctggc actggcacat      480

atgataaagt ttcgcgggca tttccttatt gagggagatt tgaatcccga taacagtgac      540

gtggataaac ttttcataca attggtccag acctataatc aactctttga agagaatcca      600

atcaacgcat ctggtgtgga cgctaaggcc attctcagtg cccggctgtc aaaatctagg      660

cgccttgaga atctgattgc acagctgccc ggggaaaaga agaacggctt gtttggaaat      720

ctgatcgctc tgtctctggg tctcacacct aatttcaagt caaatttcga cctggccgag      780

gatgcaaagc ttcagcttag caaagatacc tacgatgatg acctcgacaa tcttctcgcc      840

cagatcggcg accaatatgc tgacctgttc ctggccgcta agaatctgtc tgatgccatc      900

ctgctttctg atatcctgcg cgtcaatacc gaaataacca aggcaccact ttctgcttcc      960

atgattaaaa ggtacgacga gcatcaccaa gatctcactc tccttaaggc ccttgtgcgt     1020

cagcaactgc ccgaaaagta caaagaaatc ttctttgacc agtctaagaa tgggtacgcc     1080

ggctacatcg atggaggcgc atcccaggaa gaattttata agttcattaa gcccatactg     1140

gaaaagatgg acggcactga ggaactgctt gtcaagctga acagagaaga tcttctgcgc     1200

aaacagcgta ccttcgataa tggttctatt ccacaccaaa ttcatctggg agagctccac     1260

gccattctcc ggagacaaga ggacttttat ccattcctga aggataatcg cgagaagatc     1320

gaaaagattc tcacatttcg gattccatac tacgttggcc cccttgcccg aggtaatagc     1380

cgttttgctt ggatgaccag gaagagtgag gaaaccatta ccccttggaa ctttgaagaa     1440

gtggtagata agggagcctc cgcacaaagc tttattgagc gaatgactaa tttcgataag     1500

aatctgccaa acgaaaaagt cttgcctaaa cactccctgc tgtacgagta tttcacagtg     1560

tataacgagt tgacaaaagt caagtatgtt actgagggca tgcgaaaacc cgctttcctg     1620

tccggtgaac agaaaaaagc cattgtcgac ctgctcttta aaactaaccg taaggtcacc     1680

gtcaaacaac ttaaggaaga ctattttaag aaaatcgagt gctttgactc cgttgagatt     1740

tccggagtag aagatcgatt caacgccagt ctgggtacat atcacgacct cctgaagata     1800

atcaaagaca aggattttct ggataatgag gagaatgagg acatactgga ggacattgtc     1860

ctgaccttga ccttgttcga agaccgggaa atgattgaag agaggctgaa aacctacgcc     1920

cacctgttcg acgataaggt tatgaagcaa ctgaaacgta ggaggtacac tgggtggggc     1980

cgactttctc gtaagcttat taacggaatt agagataagc agtctggtaa gaccattctc     2040

gacttcctga aaagcgatgg ctttgctaac cgtaacttta tgcagctgat tcacgacgat     2100

tctctgacat tcaaagaaga tatccagaaa gctcaagtct ctggtcaggg cgacagcctg     2160

cacgagcaca ttgcaaacct ggccggcagc ccagctatca agaagggaat acttcaaaca     2220

gtgaaggtgg tagacgagct ggtgaaggtc atgggtcgcc acaagccaga aaacatagta     2280

atcgagatgg cacgtgaaaa ccagacaacc cagaaggggc agaagaattc tagagagcgg     2340

atgaaaagaa ttgaggaagg gatcaaggaa ctgggctctc agatcctcaa ggagcaccca     2400

gtagagaata cacagctcca aaatgaaaaa ctgtacttgt attacctgca aaacggcagg     2460

gacatgtacg tcgaccagga actggacatt aatcgcctga gtgattatga cgtcgaccat     2520

atcgtccctc agtccttcct gaaagatgat agcatcgaca acaaagtcct cactcggtcc     2580

gacaaaaacc gtggcaagag cgacaatgtc ccttctgagg aagttgtgaa gaagatgaag     2640

aattattggc gtcagctcct gaatgcaaag ttgatcactc agaggaaatt cgacaatctc     2700

actaaggctg agcgaggagg gctgtccgag ctggacaaag ccggatttat caaacgtcaa     2760

ctcgttgaga ctcggcagat cactaagcac gtcgctcaaa tccttgattc cagaatgaat     2820

accaagtacg atgaaaacga taagttgatc cgcgaggtga aagtcattac actgaagtct     2880

aagctcgtgt ctgattttcg caaggacttc caattctata aagtgaggga gatcaacaat     2940

tatcaccacg cccacgacgc ttatctcaat gctgtggtgg gcactgccct gatcaagaaa     3000

tatcccaaac tggagtcaga gtttgtgtat ggagattaca aagtgtacga tgtgaggaag     3060

atgatcgcca agagcgagca ggagatcgga aaagcaacag ccaagtactt cttttacagc     3120

aatatcatga atttctttaa gaccgagatc accttggcca atggtgagat ccgaaaacgc     3180

cctctgatag agactaatgg cgaaactggt gagattgttt gggacaaggg aagagatttc     3240

gcaactgtga ggaaggtcct gtctatgccc caggtgaata tcgttaaaaa gaccgaggtg     3300

cagactggtg gcttcagtaa ggagtctatc ctgcctaagc gcaacagcga caaactgatt     3360

gcacgaaaga aggactggga ccccaagaaa tacggagggt tcgacagccc taccgtggct     3420

tacagcgtgc ttgtggtcgc aaaggtggag aaggggaaat ctaagaagct gaaaagtgtg     3480

aaagagctgc tgggcattac cattatggaa cggagttctt tcgagaagaa ccccatcgat     3540

tttctggaag ccaagggcta taaggaagtt aaaaaggact tgattatcaa gctgcctaag     3600

tatagtttgt tcgaacttga aaacggtaga aaaaggatgc tcgcctccgc aggtgagctg     3660

cagaagggaa acgaattggc tctcccatcc aaatacgtga acttcttgta cctggccagc     3720

cattacgaaa aactcaaagg ctccccagag gacaacgaac agaagcagct gtttgtggaa     3780

caacataaac attacttgga cgaaataatc gagcagatca gcgagttttc caagagagtt     3840

atcctggccg atgcaaacct cgataaggtc ctttccgcct acaacaagca cagagacaaa     3900

ccaatccggg agcaggccga gaatattatc cacctgttca ccctgacaaa ccttggagca     3960

cctgctgcat tcaaatattt cgatactaca atcgatcgaa agcgctacac aagtaccaag     4020

gaggtgctgg acgccactct gatccatcag tccataactg gactgtatga aactcgtatc     4080

gatctgagcc agttgggggg agattctcgg gcagat                               4116


<210>  18
<211>  1372
<212>  PRT
<213>  Streptococcus pyogenes

<400>  18

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Ser Arg  Ala Asp 
    1370         


<210>  19
<211>  21
<212>  DNA
<213>  Simian virus 40

<400>  19
cccaagaaga agaggaaggt g                                                 21


<210>  20
<211>  7
<212>  PRT
<213>  Simian virus 40

<400>  20

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  21
<211>  36
<212>  DNA
<213>  Streptococcus pyogenes

<400>  21
gttttagagc tatgctgttt tgaatggtcc caaaac                                 36


<210>  22
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rituximab heavy chain amino acid sequence

<400>  22

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser 
        115                 120                 125             


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
    130                 135                 140                 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
            180                 185                 190         


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
        195                 200                 205             


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
    210                 215                 220                 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
225                 230                 235                 240 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                245                 250                 255     


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
            260                 265                 270         


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
        275                 280                 285             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
    290                 295                 300                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
305                 310                 315                 320 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
                325                 330                 335     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            340                 345                 350         


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
        355                 360                 365             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    370                 375                 380                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
385                 390                 395                 400 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
                405                 410                 415     


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
            420                 425                 430         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        435                 440                 445             


Pro Gly Lys 
    450     


<210>  23
<211>  2051
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rituximab heavy chain nucleic acid sequence

<400>  23
atgtatctgg gattgaattg cgtcattatc gtgtttctgc tcaagggtgt gcaaagtcag       60

gtccagctgc agcagccagg cgcagagctg gttaagccag gagcctcagt gaaaatgagc      120

tgcaaagcct ctggctacac ctttaccagc tataacatgc attgggtgaa acagacaccc      180

ggcagagggc tggaatggat cggagccata taccccggga acggggacac ctcctataac      240

cagaagttca agggaaaggc cacactcact gctgacaagt ccagtagcac cgcttacatg      300

caactttcaa gcttgacatc agaggattct gcagtttact actgtgcccg gtctacttac      360

tatggcggcg attggtattt caatgtatgg ggtgctggca caacagtcac tgtgagcgca      420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg      480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttggtgag      720

aggccagcac agggagggag ggtgtctgct ggaagccagg ctcagcgctc ctgcctggac      780

gcatcccggc tatgcagccc cagtccaggg cagcaaggca ggccccgtct gcctcttcac      840

ccggaggcct ctgcccgccc cactcatgct cagggagagg gtcttctggc tttttcccca      900

ggctctgggc aggcacaggc taggtgcccc taacccaggc cctgcacaca aaggggcagg      960

tgctgggctc agacctgcca agagccatat ccgggaggac cctgcccctg acctaagccc     1020

accccaaagg ccaaactctc cactccctca gctcggacac cttctctcct cccagattcc     1080

agtaactccc aatcttctct ctgcagagcc caaatcttgt gacaaaactc acacatgccc     1140

accgtgccca ggtaagccag cccaggcctc gccctccagc tcaaggcggg acaggtgccc     1200

tagagtagcc tgcatccagg gacaggcccc agccgggtgc tgacacgtcc acctccatct     1260

cttcctcagc acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca     1320

aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc     1380

acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca     1440

agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg     1500

tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc aacaaagccc     1560

tcccagcccc catcgagaaa accatctcca aagccaaagg tgggacccgt ggggtgcgag     1620

ggccacatgg acagaggccg gctcggcccc ataacttcgt atagcataca ttatacgaag     1680

ttataccctc tgccctgaga gtgaccgctg taccaacctc tgtccctaca gggcagcccc     1740

gagaaccaca ggtgtacacc ctgcccccat cccgggatga gctgaccaag aaccaggtca     1800

gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca     1860

atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct     1920

tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct     1980

catgctccgt gatgcatgag gctctgcaca accactacac gcagaagagc ctctccctgt     2040

ctccgggtaa a                                                          2051


<210>  24
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rituximab light chain amino acid sequence

<400>  24

Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Ile 
            20                  25                  30          


His Trp Phe Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr 
        35                  40                  45              


Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Thr Ser Asn Pro Pro Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro 
            100                 105                 110         


Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr 
        115                 120                 125             


Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys 
    130                 135                 140                 


Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu 
145                 150                 155                 160 


Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser 
                165                 170                 175     


Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala 
            180                 185                 190         


Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe 
        195                 200                 205             


Asn Arg Gly Glu Cys 
    210             


<210>  25
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rituximab light chain nucleic acid sequence

<400>  25
atggatatga gggtaccagc acaacttctc ggattactat tgttatggct gcgaggtgcg       60

cgctgtcaga ttgtcttgag ccagtctccc gccattttgt ctgcctcccc tggggagaaa      120

gtaaccatga cttgtcgcgc atcctcaagc gtgagttaca tccactggtt tcagcagaag      180

cctggcagct cacccaagcc ctggatctat gctacctcca acctcgcttc cggagtgcct      240

gtgcggtttt ctgggtccgg tagtggtacc agctactcac tgactatttc aagagttgag      300

gctgaagatg ccgcaaccta ttactgccaa cagtggacaa gtaatccacc aacattcggt      360

ggcggcacta aactggagat caagcgtacg gtggctgcac catctgtctt catcttcccg      420

ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc      480

tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc      540

caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg      600

acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag      660

ggcctgagtt caccggtgac aaagagcttc aacaggggag agtgt                      705


<210>  26
<211>  469
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trastuzumab heavy chain amino acid sequence

<400>  26

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile 
        35                  40                  45              


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
                85                  90                  95      


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Lys 
465                 


<210>  27
<211>  2008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trastuzumab heavy chain nucleic acid sequence

<400>  27
atggagctgt atcatcctct tcttggtagc aacagctaca ggtgtccact ccgaggttca       60

gctggtggag tctggcggtg gcctggtgca gccagggggc tcactccgtt tgtcctgtgc      120

agcttctggc ttcaacatta aagacaccta tatacactgg gtgcgtcagg ccccgggtaa      180

gggcctggaa tgggttgcaa ggatttatcc tacgaatggt tatactagat atgccgatag      240

cgtcaagggc cgtttcacta taagcgcaga cacatccaaa aacacagcct acctgcagat      300

gaacagcctg cgtgctgagg acactgccgt ctattattgt tccagatggg gaggggacgg      360

cttctatgct atggactact ggggtcaagg aaccctggtc accgtctcct cggctagcac      420

caagggccca tcggtcttcc ccctggcacc ctcctccaag agcacctctg ggggcacagc      480

ggccctgggc tgcctggtca aggactactt ccccgaaccg gtgacggtgt cgtggaactc      540

aggcgccctg accagcggcg tgcacacctt cccggctgtc ctacagtcct caggactcta      600

ctccctcagc agcgtggtga ccgtgccctc cagcagcttg ggcacccaga cctacatctg      660

caacgtgaat cacaagccca gcaacaccaa ggtggacaag aaagttggtg agaggccagc      720

acagggaggg agggtgtctg ctggaagcca ggctcagcgc tcctgcctgg acgcatcccg      780

gctatgcagc cccagtccag ggcagcaagg caggccccgt ctgcctcttc acccggaggc      840

ctctgcccgc cccactcatg ctcagggaga gggtcttctg gctttttccc caggctctgg      900

gcaggcacag gctaggtgcc cctaacccag gccctgcaca caaaggggca ggtgctgggc      960

tcagacctgc caagagccat atccgggagg accctgcccc tgacctaagc ccaccccaaa     1020

ggccaaactc tccactccct cagctcggac accttctctc ctcccagatt ccagtaactc     1080

ccaatcttct ctctgcagag cccaaatctt gtgacaaaac tcacacatgc ccaccgtgcc     1140

caggtaagcc agcccaggcc tcgccctcca gctcaaggcg ggacaggtgc cctagagtag     1200

cctgcatcca gggacaggcc ccagccgggt gctgacacgt ccacctccat ctcttcctca     1260

gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc     1320

ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac     1380

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag     1440

ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac     1500

caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc     1560

cccatcgaga aaaccatctc caaagccaaa ggtgggaccc gtggggtgcg agggccacat     1620

ggacagaggc cggctcggcc caccctctgc cctgagagtg accgctgtac caacctctgt     1680

ccctacaggg cagccccgag aaccacaggt gtacaccctg cccccatccc gggaggagat     1740

gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc     1800

cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct     1860

ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca     1920

gcaggggaac gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca     1980

gaagagcctc tccctgtctc cgggtaaa                                        2008


<210>  28
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trastuzumab light chain amino acid sequence

<400>  28

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
            20                  25                  30          


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val 
        35                  40                  45              


Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
    50                  55                  60                  


Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                85                  90                  95      


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr 
            100                 105                 110         


Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
        115                 120                 125             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    130                 135                 140                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
145                 150                 155                 160 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                165                 170                 175     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            180                 185                 190         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        195                 200                 205             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    210                 215                 220                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230             


<210>  29
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trastuzumab light chain nucleic acid sequence

<400>  29
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctacaggtgt ccactccgat       60

attcagatga cccagtcccc gagctccctg tccgcctctg tgggcgatag ggtcaccatc      120

acctgccgtg ccagtcagga tgtgaatact gctgtagcct ggtatcaaca gaaaccagga      180

aaagctccga aactactgat ttactcggca tccttcctct actctggagt cccttctcgc      240

ttctctggtt ccagatctgg gacggatttc actctgacca tcagcagtct gcagccggaa      300

gacttcgcaa cttattactg tcagcaacat tatactactc ctcccacgtt cggacagggt      360

accaaggtgg agatcaaacg tacggtggct gcaccatctg tcttcatctt cccgccatct      420

gatgagcagt tgaaatctgg aactgcctct gttgtgtgcc tgctgaataa cttctatccc      480

agagaggcca aagtacagtg gaaggtggat aacgccctcc aatcgggtaa ctcccaggag      540

agtgtcacag agcaggacag caaggacagc acctacagcc tcagcagcac cctgacgctg      600

agcaaagcag actacgagaa acacaaagtc tacgcctgcg aagtcaccca tcagggcctg      660

agctcgcccg tcacaaagag cttcaacagg ggagagtgt                             699


