                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
 
<120>  MOLECULAR AND CELLULAR IMAGING USING ENGINEERED HEMODYNAMIC 
       RESPONSES

<130>  M0925.70388WO00

<140>  Not Yet Assigned
<141>  2014-07-15

<150>  US 61/846,232
<151>  2013-07-15

<160>  22   

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  AMIDATION

<400>  1

Ala Cys Asp Thr Ala Thr Cys Val Thr His Arg Leu Ala Gly Leu Leu 
1               5                   10                  15      


Ser Arg Ser Gly Gly Val Val Lys Asn Asn Phe Val Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Ala Phe 
        35          


<210>  2
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  2

Met Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Ala 
1               5                   10              


<210>  3
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  3

Gly Gly Ser Gly Gly Pro Leu Gly Ile Ala Gly 
1               5                   10      


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  4

Gly Gly Ser Gly Asp Glu Val Asp 
1               5               


<210>  5
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  5

Gly Gly Ser Gly Ile Glu Gly Arg 
1               5               


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  6

Gly Gly Ser Gly Glu Asn Leu Tyr Phe Gln Gly 
1               5                   10      


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  7

Gly Gly Ser Gly Asp Asp Asp Asp Lys 
1               5                   


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  8

Gly Pro Leu Gly Ile Ala Gly 
1               5           


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  9

Asp Glu Val Asp 
1               


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  10

Ile Glu Gly Arg 
1               


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  11

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  12

Asp Asp Asp Asp Lys 
1               5   


<210>  13
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  13

Gly Gly Ser Gly 
1               


<210>  14
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  StrepII-GFP


<220>
<221>  misc_feature
<222>  (1)..(13)
<223>  StrepII affinity tag

<220>
<221>  misc_feature
<222>  (14)..(251)
<223>  cfSGFP2 blocking domain

<400>  14

Met Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Ala Val Ser Lys 
1               5                   10                  15      


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
            20                  25                  30          


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
        35                  40                  45              


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Ser Thr Thr Gly 
    50                  55                  60                  


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
65                  70                  75                  80  


Val Gln Met Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
                85                  90                  95      


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
            100                 105                 110         


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
        115                 120                 125             


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
    130                 135                 140                 


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
145                 150                 155                 160 


His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala 
                165                 170                 175     


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala 
            180                 185                 190         


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
        195                 200                 205             


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro 
    210                 215                 220                 


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
225                 230                 235                 240 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                245                 250     


<210>  15
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  haCGRP-Gly

<400>  15

Ala Cys Asp Thr Ala Thr Cys Val Thr His Arg Leu Ala Gly Leu Leu 
1               5                   10                  15      


Ser Arg Ser Gly Gly Val Val Lys Asn Asn Phe Val Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Ala Phe Gly 
        35              


<210>  16
<211>  3154
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEXPR-T7-cfSGFP2-Linker-Cleavage-Site-CGRP-Gly


<220>
<221>  misc_feature
<222>  (1)..(117)
<223>  haCGRP-Gly

<220>
<221>  misc_feature
<222>  (172)..(215)
<223>  T7 terminator

<220>
<221>  misc_feature
<222>  (367)..(1176)
<223>  KanR: Kanamycin resistance gene

<220>
<221>  misc_feature
<222>  (1250)..(1869)
<223>  pBR322 origin of replication

<220>
<221>  misc_feature
<222>  (2022)..(2065)
<223>  rrnB T1 terminator

<220>
<221>  misc_feature
<222>  (2197)..(2224)
<223>  rrnB T2 terminator

<220>
<221>  misc_feature
<222>  (2297)..(2315)
<223>  T7 promoter	

<220>
<221>  misc_feature
<222>  (2353)..(2369)
<223>  RBS: ribosome binding site

<220>
<221>  misc_feature
<222>  (2378)..(2416)
<223>  StrepII affinity tag

<220>
<221>  misc_feature
<222>  (2417)..(3130)
<223>  cfSGFP2: Cysteine-free GFP 

<220>
<221>  misc_feature
<222>  (3131)..(3142)
<223>  Linker: GGSG

<220>
<221>  misc_feature
<222>  (3143)..(3154)
<223>  Caspase-3 cleavage site: DEVD

<400>  16
gcttgtgaca cagccacctg tgtcacccac cgcctggccg gtctgctgtc tcgctcgggt       60

ggcgttgtta aaaacaattt tgtgccaacc aatgttggtt caaaagcgtt tggctaacaa      120

agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct      180

tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata      240

tccacaggac ggaggtagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa      300

gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg      360

ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat      420

gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc      480

tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc      540

gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct      600

cttccgacca tcaagcattt tatccgtact cctggtgatg catggttact caccactgcg      660

atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt      720

gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct      780

tttaacagcg atcgcgtatt tcgcctcgct caggcgcaat cacgaatgaa taacggtttg      840

gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa      900

gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca      960

cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc     1020

ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct     1080

ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa     1140

ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa     1200

cattattcag attgggcccc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     1260

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     1320

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     1380

ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac     1440

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     1500

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     1560

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     1620

acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc     1680

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     1740

agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     1800

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc     1860

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     1920

cctgcgttat cccctgattc tgtggataac cgtattaccg ctagcatgga tctcggggac     1980

gtctaactac taagcgagag tagggaactg ccaggcatca aataaaacga aaggcccagt     2040

cttccgactg agcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga     2100

caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag     2160

gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc     2220

tttttgcgtt tctacaaact cttcctgtta gttagttact taagctcaga ggatcgagat     2280

ctcgatcccg cgaaattaat acgactcact atagggagag ccacaacggt ttccctctag     2340

aaataatttt gtttaacttt aagaaggaga tatacatatg agcgcgtgga gccatccgca     2400

gtttgaaaaa ggtgctgtat ccaagggcga agagctgttc acgggcgtag taccgatttt     2460

ggtggagctg gacggcgatg tgaacggcca taaatttagt gtgagcggcg aaggtgaggg     2520

cgatgcgaca tatggcaaac tgaccctgaa atttattagt accaccggta aactgccagt     2580

gccttggccg acgctggtta ccaccctgac ctacggtgtg caaatgttcg ctcgctaccc     2640

cgaccatatg aaacagcatg acttcttcaa gagcgcaatg ccggaaggtt acgtgcagga     2700

gcgtacgatt tttttcaaag atgatgggaa ttacaagaca cgtgcggaag tgaaattcga     2760

gggcgatacg ctggttaatc gcatcgaact gaaaggcatc gacttcaaag aagatggtaa     2820

cattctgggt cacaagcttg aatacaacta caactcacat aacgtatata tcacagcaga     2880

taagcagaag aatggtatta aggcgaattt caagatccgt cacaatattg aggatggcgg     2940

tgttcagtta gctgatcatt atcaacagaa cacccctatt ggagatggcc cagtgttgct     3000

gccagataat cattacctgt ctacccagag caaactgagc aaagacccta atgagaaacg     3060

tgatcatatg gtcttactgg agttcgttac agcggcgggc attacgctgg gtatggatga     3120

gctgtataag ggtggctcag gtgatgaagt agat                                 3154


<210>  17
<211>  12014
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLV-hEF1a-Glo22F-IRES-H2B-Cerulean-2A-puro


<220>
<221>  misc_feature
<222>  (41)..(1224)
<223>  hEF1a: Human elongation factor 1 alpha promoter

<220>
<221>  misc_feature
<222>  (1269)..(3374)
<223>  Glo22F: Engineered cAMP-responsive luciferase

<220>
<221>  misc_feature
<222>  (3375)..(3976)
<223>  IRES: Internal ribosome entry site

<220>
<221>  misc_feature
<222>  (3977)..(5095)
<223>  DNA binding domain of histone 2B fused to Cerulean

<220>
<221>  misc_feature
<222>  (5096)..(5149)
<223>  2A: Autoproteolytic viral amino acid sequence

<220>
<221>  misc_feature
<222>  (5150)..(5749)
<223>  Puro: Puromycin resistance gene

<220>
<221>  misc_feature
<222>  (7307)..(7359)
<223>  delta_U3: Deletion in the U3 region of 3'LTR	

<220>
<221>  misc_feature
<222>  (7360)..(7540)
<223>  LTR: HIV long terminal repeat

<220>
<221>  misc_feature
<222>  (7917)..(7945)
<223>  AmpR promoter

<220>
<221>  misc_feature
<222>  (7987)..(8847)
<223>  AmpR: Ampicillin resistance gene

<220>
<221>  misc_feature
<222>  (8998)..(9662)
<223>  pUC ori: pUC origin of replication

<220>
<221>  misc_feature
<222>  (10067)..(10486)
<223>  Rous Sarcoma Virus / HIV 5'LTR hybrid promoter

<220>
<221>  misc_feature
<222>  (10303)..(10483)
<223>  LTR: HIV long terminal repeat

<220>
<221>  misc_feature
<222>  (10591)..(10635)
<223>  Psi: Lentiviral Psi packaging sequence

<220>
<221>  misc_feature
<222>  (11145)..(11378)
<223>  RRE: Rev response element

<220>
<221>  misc_feature
<222>  (11867)..(11984)
<223>  CPPT: Central polypurine tract

<400>  17
ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt       60

cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat      120

tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg      180

ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac      240

gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc      300

gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg      360

cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct      420

tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc      480

cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta      540

gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta      600

aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg      660

gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga      720

atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg      780

tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa      840

agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga      900

gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct      960

tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt     1020

tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact     1080

gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt     1140

gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt     1200

tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactatagcc     1260

tggccaccat gcctggcgca gtaggcaagg tggtgccctt catcgaggct aaggtggtgg     1320

acttggacac tggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc     1380

ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg     1440

acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg     1500

tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg     1560

agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg     1620

acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg     1680

agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg     1740

gtgttgtgtt agtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga     1800

tccgcgagat tctcattaag gccaagaagg gatccaattg ggattctggg tgctccagag     1860

aaggtatgta tgaaagcttt attgagtcac tgccattcct taaatctttg gagttttctg     1920

cacgcctgaa agtagtagat gtgataggca ccaaagtata caacgatgga gaacaaatca     1980

ttgctcaggg agattcggct gattcttttt tcattattga atctggagaa gtgaaaatta     2040

ctatgaaaag aaagggtaaa tcagaagtgg aagagaatgg tgcagtagaa atcgctcgat     2100

gctcgcgggg acagtacttt ggagagcttg ccctggtaac taacaaacct cgagcagctt     2160

ctgcccacgc cattgggact gtcaaatgtt tagcaatgga tgtgcaagca tttgaaaggc     2220

ttctgggacc ttgcatggaa attatgaaaa ggaacatcgc tacctatgaa gaacagttag     2280

ttgccctgta tggaacgaac atggatattg tagccaaaaa cattaagaag ggcccagcgc     2340

cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct     2400

acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct     2460

acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga     2520

atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt     2580

tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg     2640

agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc     2700

tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg     2760

atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc     2820

cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg     2880

ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc     2940

gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc     3000

ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc     3060

tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat     3120

tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta     3180

gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg     3240

ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc     3300

taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcactc     3360

cagaaggggt ttaagactgg gatccgcccc tctccctccc ccccccctaa cgttactggc     3420

cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg     3480

ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct     3540

aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca     3600

gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg     3660

aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct     3720

gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa     3780

tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt     3840

atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa     3900

aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa     3960

tatggccaca gccaccatgc cagagccagc gaagtctgct cccgccccga aaaagggctc     4020

caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa     4080

ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat     4140

ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc     4200

aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat     4260

ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg     4320

tactaaggcc atcaccaagt acaccagcgc taaggatccc cgggtaccgg tcgccaccat     4380

ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg     4440

cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg     4500

caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct     4560

cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca     4620

gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt     4680

caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt     4740

gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa     4800

gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg     4860

catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga     4920

ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta     4980

cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct     5040

gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaggg     5100

ccgcggcagc ctgctgacct gcggcgacgt ggaggaaaac cccggcccca tgaccgagta     5160

caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc     5220

cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga     5280

gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt     5340

gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc     5400

gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc     5460

cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt     5520

cctggccacc gtcggcgtgt cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt     5580

gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc     5640

gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt     5700

gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag ggacaggtga     5760

tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga     5820

ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg     5880

cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc     5940

tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc     6000

actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt     6060

tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt     6120

gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg     6180

aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg     6240

tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg     6300

ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt     6360

tgggccgcct ccccgcctgg aattctgcag atatccggtt agtaatgagt ttggaattaa     6420

ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga     6480

agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc     6540

ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc     6600

ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc     6660

tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag     6720

aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cctgttgaca     6780

attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca     6840

tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg     6900

agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg     6960

tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca     7020

acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg     7080

tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc     7140

cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg     7200

aggagcagga ctgacacgtg ctacgagatt taaatggtac ctttaagacc aatgacttac     7260

aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct     7320

cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag     7380

atctgagcct gggagctctc tggctgccta gggaacccac tgcttaagcc tcaataaagc     7440

ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga     7500

tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt     7560

attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt     7620

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     7680

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     7740

tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct     7800

gactaatttt ttttatttat gcagaggccg aggccgacgt gtgtttctta gacgtcaggt     7860

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     7920

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg     7980

aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc     8040

cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg     8100

ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt     8160

cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta     8220

ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat     8280

gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga     8340

gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca     8400

acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact     8460

cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc     8520

acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact     8580

ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt     8640

ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt     8700

gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt     8760

atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     8820

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     8880

attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat     8940

ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     9000

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     9060

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     9120

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg     9180

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     9240

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     9300

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     9360

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc     9420

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     9480

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     9540

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     9600

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     9660

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     9720

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     9780

gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc     9840

agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg     9900

agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg     9960

tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc    10020

aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt    10080

cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac    10140

aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct    10200

tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt    10260

gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct ctggttagac    10320

cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa    10380

agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag    10440

agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg    10500

acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt gctgaagcgc    10560

gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag    10620

gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc    10680

gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag    10740

tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag    10800

aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac    10860

ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa    10920

aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg    10980

cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga    11040

agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag    11100

gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt    11160

gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct gacggtacag    11220

gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag    11280

gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc    11340

ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga    11400

aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa    11460

cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct    11520

taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat    11580

tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt    11640

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg    11700

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc    11760

tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag    11820

acagagacag atccattcga ttagtgaacg gatctcgacg gttaactttt aaaagaaaag    11880

gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac    11940

aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttattcca gtgtggtgga    12000

attctgcagt ctgc                                                      12014


<210>  18
<211>  11354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLV-hEF1a-HA-CALCRL-IRES-EYFP-2A-Hygro

<220>
<221>  misc_feature
<222>  (41)..(1224)
<223>  hEF1a: Human elongation factor 1 alpha promoter

<220>
<221>  misc_feature
<222>  (1269)..(2669)
<223>  HA-CALCRL

<220>
<221>  misc_feature
<222>  (2670)..(3271)
<223>  IRES: Internal ribosome entry site

<220>
<221>  misc_feature
<222>  (3272)..(3988)
<223>  EYFP: Enhanced yellow fluorescent protein

<220>
<221>  misc_feature
<222>  (3995)..(4048)
<223>  2A: Autoproteolytic viral amino acid sequence

<220>
<221>  misc_feature
<222>  (4049)..(5089)
<223>  Hygro: Hygromycin resistance gene

<220>
<221>  misc_feature
<222>  (6647)..(6699)
<223>  delta_U3: Deletion in the U3 region of 3'LTR

<220>
<221>  misc_feature
<222>  (6700)..(6880)
<223>  LTR: HIV long terminal repeat

<220>
<221>  misc_feature
<222>  (7257)..(7285)
<223>  AmpR promoter

<220>
<221>  misc_feature
<222>  (7327)..(8187)
<223>  AmpR: Ampicillin resistance gene

<220>
<221>  misc_feature
<222>  (8338)..(9002)
<223>  pUC ori: pUC origin of replication

<220>
<221>  misc_feature
<222>  (9407)..(9826)
<223>  Rous Sarcoma Virus / HIV 5'LTR hybrid promoter

<220>
<221>  misc_feature
<222>  (9643)..(9823)
<223>  LTR: HIV long terminal repeat

<220>
<221>  misc_feature
<222>  (9931)..(9975)
<223>  Psi: Lentiviral Psi packaging sequence

<220>
<221>  misc_feature
<222>  (10485)..(10718)
<223>  RRE: Rev response element

<220>
<221>  misc_feature
<222>  (11207)..(11324)
<223>  CPPT: Central polypurine tract

<400>  18
ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt       60

cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat      120

tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg      180

ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac      240

gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc      300

gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg      360

cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct      420

tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc      480

cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta      540

gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta      600

aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg      660

gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga      720

atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg      780

tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa      840

agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga      900

gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct      960

tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt     1020

tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact     1080

gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt     1140

gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt     1200

tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactatagcc     1260

tggccaccat gaagaccatc ctggccctga gcacctacat cttctgcctg gtgttcgcct     1320

acccctacga cgtgcccgtg tacgccgcag aattagaaga gagtcctgag gactcaattc     1380

agttgggagt tactagaaat aaaatcatga cagctcaata tgaatgttac caaaagatta     1440

tgcaagaccc cattcaacaa gcagaaggcg tttactgcaa cagaacctgg gatggatggc     1500

tctgctggaa cgatgttgca gcaggaactg aatcaatgca gctctgccct gattactttc     1560

aggactttga tccatcagaa aaagttacaa agatctgtga ccaagatgga aactggttta     1620

gacatccagc aagcaacaga acatggacaa attataccca gtgtaatgtt aacacccacg     1680

agaaagtgaa gactgcacta aatttgtttt acctgaccat aattggacac ggattgtcta     1740

ttgcatcact gcttatctcg cttggcatat tcttttattt caagagccta agttgccaaa     1800

ggattacctt acacaaaaat ctgttcttct catttgtttg taactctgtt gtaacaatca     1860

ttcacctcac tgcagtggcc aacaaccagg ccttagtagc cacaaatcct gttagttgca     1920

aagtgtccca gttcattcat ctttacctga tgggctgtaa ttacttttgg atgctctgtg     1980

aaggcattta cctacacaca ctcattgtgg tggccgtgtt tgcagagaag caacatttaa     2040

tgtggtatta ttttcttggc tggggatttc cactgattcc tgcttgtata catgccattg     2100

ctagaagctt atattacaat gacaattgct ggatcagttc tgatacccat ctcctctaca     2160

ttatccatgg cccaatttgt gctgctttac tggtgaatct ttttttcttg ttaaatattg     2220

tacgcgttct catcaccaag ttaaaagtta cacaccaagc ggaatccaat ctgtacatga     2280

aagctgtgag agctactctt atcttggtgc cattgcttgg cattgaattt gtgctgattc     2340

catggcgacc tgaaggaaag attgcagagg aggtatatga ctacatcatg cacatcctta     2400

tgcacttcca gggtcttttg gtgtctacca ttttctgctt ctttaatgga gaggttcaag     2460

caattctgag aagaaactgg aatcaataca aaatccaatt tggaaacagc ttttccaact     2520

cagaagctct tcgtagtgcg tcttacacag tgtcaacaat cagtgatggt ccaggttata     2580

gtcatgactg tcctagtgaa cacttaaatg gaaaaagcat ccatgatatt gaaaatgttc     2640

tcttaaaacc agaaaattta tataattgag actgggatcc gcccctctcc ctcccccccc     2700

cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta     2760

ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc     2820

ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat     2880

gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc     2940

ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt     3000

gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt     3060

gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag     3120

aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt     3180

tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga     3240

aaaacacgat gataatatgg ccacagccac catggtgagc aagggcgagg agctgttcac     3300

cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt     3360

gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac     3420

caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca     3480

gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc     3540

cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg     3600

cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga     3660

cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa     3720

cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca     3780

caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg     3840

cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa     3900

agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat     3960

cactctcggc atggacgagc tgtacaagca attggagggc cgcggcagcc tgctgacctg     4020

cggcgacgtg gaggaaaacc ccggccccat gaaaaagcct gaactcaccg cgacgtctgt     4080

cgagaagttt ctgatcgaaa agttcgacag cgtgtccgac ctgatgcagc tctcggaggg     4140

cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa     4200

tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc     4260

gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat     4320

ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt     4380

tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag     4440

cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat     4500

atgcgcgatt gctgatcccc atgtgtatca ctcctggcaa actgtgatgg acgacaccgt     4560

cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga     4620

agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg     4680

cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc     4740

caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga     4800

gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg     4860

tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca     4920

gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc     4980

ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa     5040

ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgag ggacaggtga     5100

tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga     5160

ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg     5220

cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc     5280

tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc     5340

actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt     5400

tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt     5460

gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg     5520

aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg     5580

tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg     5640

ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt     5700

tgggccgcct ccccgcctgg aattctgcag atatccggtt agtaatgagt ttggaattaa     5760

ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga     5820

agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc     5880

ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc     5940

ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc     6000

tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag     6060

aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cctgttgaca     6120

attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca     6180

tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg     6240

agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg     6300

tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca     6360

acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg     6420

tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc     6480

cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg     6540

aggagcagga ctgacacgtg ctacgagatt taaatggtac ctttaagacc aatgacttac     6600

aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct     6660

cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag     6720

atctgagcct gggagctctc tggctgccta gggaacccac tgcttaagcc tcaataaagc     6780

ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga     6840

tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt     6900

attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt     6960

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     7020

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     7080

tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct     7140

gactaatttt ttttatttat gcagaggccg aggccgacgt gtgtttctta gacgtcaggt     7200

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     7260

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg     7320

aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc     7380

cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg     7440

ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt     7500

cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta     7560

ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat     7620

gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga     7680

gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca     7740

acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact     7800

cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc     7860

acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact     7920

ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt     7980

ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt     8040

gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt     8100

atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     8160

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     8220

attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat     8280

ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     8340

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     8400

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     8460

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg     8520

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     8580

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     8640

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     8700

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc     8760

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     8820

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     8880

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     8940

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     9000

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     9060

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     9120

gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc     9180

agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg     9240

agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg     9300

tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc     9360

aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt     9420

cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac     9480

aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct     9540

tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt     9600

gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct ctggttagac     9660

cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa     9720

agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag     9780

agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg     9840

acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt gctgaagcgc     9900

gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag     9960

gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc    10020

gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag    10080

tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag    10140

aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac    10200

ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa    10260

aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg    10320

cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga    10380

agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag    10440

gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt    10500

gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct gacggtacag    10560

gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag    10620

gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc    10680

ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga    10740

aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa    10800

cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct    10860

taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat    10920

tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt    10980

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg    11040

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc    11100

tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag    11160

acagagacag atccattcga ttagtgaacg gatctcgacg gttaactttt aaaagaaaag    11220

gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac    11280

aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttattcca gtgtggtgga    11340

attctgcagt ctgc                                                      11354


<210>  19
<211>  9749
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLV-hEF1a-myc-RAMP1-IRES-mKate-2A-Bla


<220>
<221>  misc_feature
<222>  (41)..(1224)
<223>  hEF1a: Human elongation factor 1 alpha promoter

<220>
<221>  misc_feature
<222>  (1269)..(1721)
<223>  myc-RAMP

<220>
<221>  misc_feature
<222>  (1722)..(2323)
<223>  IRES: Internal ribosome entry site

<220>
<221>  misc_feature
<222>  (2324)..(3028)
<223>  mKate2: Red fluorescent prote

<220>
<221>  misc_feature
<222>  (3029)..(3085)
<223>  2A: Autoproteolytic viral amino acid sequence

<220>
<221>  misc_feature
<222>  (3086)..(3484)
<223>  Bla: Blasticidin resistance gene

<220>
<221>  misc_feature
<222>  (5042)..(5094)
<223>  delta_U3: Deletion in the U3 region of 3'LTR

<220>
<221>  misc_feature
<222>  (5095)..(5275)
<223>  LTR: HIV long terminal repeat

<220>
<221>  misc_feature
<222>  (5652)..(5680)
<223>  AmpR promoter

<220>
<221>  misc_feature
<222>  (5722)..(6582)
<223>  AmpR: Ampicillin resistance gene

<220>
<221>  misc_feature
<222>  (6733)..(7397)
<223>  pUC ori: pUC origin of replication

<220>
<221>  misc_feature
<222>  (7802)..(8221)
<223>  Rous Sarcoma Virus / HIV 5'LTR hybrid promoter

<220>
<221>  misc_feature
<222>  (8038)..(8218)
<223>  LTR: HIV long terminal repeat

<220>
<221>  misc_feature
<222>  (8326)..(8370)
<223>  Psi: Lentiviral Psi packaging sequence

<220>
<221>  misc_feature
<222>  (8880)..(9113)
<223>  RRE: Rev response element

<220>
<221>  misc_feature
<222>  (9602)..(9719)
<223>  CPPT: Central polypurine tract

<400>  19
ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt       60

cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat      120

tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg      180

ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac      240

gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc      300

gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg      360

cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct      420

tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc      480

cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta      540

gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta      600

aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg      660

gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga      720

atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg      780

tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa      840

agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga      900

gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct      960

tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt     1020

tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact     1080

gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt     1140

gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt     1200

tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactatagcc     1260

tggccaccat gaagaccatc ctggccctga gcacctacat cttctgcctg gtgttcgccg     1320

agcagaagct gatcagcgag gaggacctgg cctgccagga ggctaactac ggtgccctcc     1380

tccgggagct ctgcctcacc cagttccagg tagacatgga ggccgtcggg gagactctgt     1440

ggtgtgactg gggcaggacc atcaggagct acagggagct ggccgactgc acctggcaca     1500

tggcggagaa gctgggctgc ttctggccca atgcagaggt ggacaggttc ttcctggcag     1560

tgcatggccg ctacttcagg agctgcccca tctcaggcag ggccgtgcgg gacccgcccg     1620

gcagcatcct ctaccccttc atcgtggtcc ccatcacggt gaccctgctg gtgacggcac     1680

tggtggtctg gcagagcaag cgcactgagg gcattgtgta ggactgggat ccgcccctct     1740

ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt     1800

gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct     1860

ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa     1920

ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg     1980

tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc     2040

caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg     2100

agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg     2160

aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc     2220

tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg     2280

gttttccttt gaaaaacacg atgataatat ggccacagcc accatggtga gcgagctgat     2340

taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa     2400

gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggc     2460

ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg     2520

cagcaaaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc     2580

cgagggcttc acatgggaga gagtcaccac atacgaagat gggggcgtgc tgaccgctac     2640

ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa     2700

cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga     2760

gacactgtac cccgctgacg gcggcctgga aggcagagcc gacatggccc tgaagctcgt     2820

gggcgggggc cacctgatct gcaaccttaa gaccacatac agatccaaga aacccgctaa     2880

gaacctcaag atgcccggcg tctactatgt ggacaggaga ctggaaagaa tcaaggaggc     2940

cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc     3000

tagcaaactg gggcacaaac ttaattccgc tgagggccgc ggcagcctgc tgacctgcgg     3060

cgacgtggag gaaaacccag gcccaatggc taagcctttg tctcaagaag aatccaccct     3120

cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc     3180

cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac     3240

tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa     3300

cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg     3360

gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga     3420

tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg     3480

ataagggaca ggtgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac     3540

aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga     3600

tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc     3660

tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa     3720

cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc     3780

acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc     3840

atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc     3900

gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg     3960

attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct     4020

tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg     4080

agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc cggttagtaa     4140

tgagtttgga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct     4200

ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga     4260

aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca     4320

accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat     4380

tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc     4440

tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag     4500

ctccccctgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg     4560

tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg     4620

tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg     4680

acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc     4740

aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg     4800

ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg     4860

agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg     4920

tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttaaat ggtaccttta     4980

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga     5040

ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct     5100

ctctggttag accagatctg agcctgggag ctctctggct gcctagggaa cccactgctt     5160

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     5220

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt     5280

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt     5340

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat     5400

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat     5460

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt     5520

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gacgtgtgtt     5580

tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt     5640

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa     5700

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt     5760

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat     5820

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag     5880

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg     5940

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata     6000

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat     6060

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc     6120

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg     6180

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac     6240

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact     6300

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     6360

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     6420

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     6480

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     6540

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac     6600

tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag     6660

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg     6720

tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc     6780

tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag     6840

ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc     6900

cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac     6960

ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc     7020

gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt     7080

tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt     7140

gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc     7200

ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt     7260

tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca     7320

ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt     7380

tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt     7440

attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag     7500

tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg     7560

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc     7620

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt     7680

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat     7740

gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg     7800

caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt     7860

agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt     7920

ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca     7980

ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg     8040

tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg     8100

cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt     8160

gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt     8220

ggcgcccgaa cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc     8280

ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     8340

ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     8400

ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     8460

aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     8520

tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     8580

caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     8640

aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     8700

aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg     8760

agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga     8820

gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata     8880

ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg     8940

acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg     9000

ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag     9060

ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt     9120

tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt     9180

aataaatctc tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta     9240

acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga     9300

atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa     9360

caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa     9420

gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat     9480

cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag     9540

aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa     9600

cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa     9660

tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta     9720

ttccagtgtg gtggaattct gcagtctgc                                       9749


<210>  20
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  20

Gly Ser Gly Gly Ser 
1               5   


<210>  21
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  21

Gly Gly Gly Gly Ser 
1               5   


<210>  22
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  22

Gly Gly Gly Ser 
1               
