               SEQUENCE LISTING

<110> Global Bioenergies
      Scientist of Fortune S.A.

<120> Mevalonate diphosphate decarboxylase variants

<130> T2557 PCT S3

<150> EP 13 17 5790.8
<151> 2013-07-09

<160> 4

<170> BiSSAP 1.2

<210> 1
<211> 317
<212> PRT
<213> Streptococcus mitis B6


<400> 1
Met Asp Arg Glu Pro Val Thr Val Arg Ser Tyr Ala Asn Ile Ala Ile 
1               5                   10                  15      
Ile Lys Tyr Trp Gly Lys Lys Lys Glu Lys Glu Met Val Pro Ala Thr 
            20                  25                  30          
Ser Ser Ile Ser Leu Thr Leu Glu Asn Met Tyr Thr Glu Thr Thr Leu 
        35                  40                  45              
Ser Ser Leu Pro Thr Asp Ala Thr Ala Asp Ala Phe Tyr Ile Asn Gly 
    50                  55                  60                  
Gln Leu Gln Asn Glu Ala Glu His Val Lys Met Ser Lys Ile Ile Asp 
65                  70                  75                  80  
Arg Tyr Arg Pro Asp Gly Asp Gly Phe Val Arg Ile Asp Thr Gln Asn 
                85                  90                  95      
Ser Met Pro Thr Ala Ala Gly Leu Ser Ser Ser Ser Ser Gly Leu Ser 
            100                 105                 110         
Ala Leu Val Lys Ala Cys Asn Ala Tyr Phe Lys Leu Gly Leu Asn Arg 
        115                 120                 125             
Ser Gln Leu Ala Gln Glu Ala Lys Phe Ala Ser Gly Ser Ser Ser Arg 
    130                 135                 140                 
Ser Phe Tyr Gly Pro Leu Gly Ala Trp Asp Lys Asp Ser Gly Glu Ile 
145                 150                 155                 160 
Tyr Pro Val Glu Thr Gly Leu Lys Leu Ala Met Ile Met Leu Val Leu 
                165                 170                 175     
Glu Asp Lys Lys Lys Pro Ile Ser Ser Arg Asp Gly Met Lys Leu Cys 
            180                 185                 190         
Val Glu Thr Ser Thr Thr Phe Asp Asp Trp Val Arg Gln Ser Glu Lys 
        195                 200                 205             
Asp Tyr Gln Asp Met Leu Val Tyr Leu Lys Ala Asn Asp Phe Ala Lys 
    210                 215                 220                 
Val Gly Glu Leu Thr Glu Lys Asn Ala Leu Ala Met His Ala Thr Thr 
225                 230                 235                 240 
Lys Thr Ala Ser Pro Ala Phe Ser Tyr Leu Thr Asp Ala Ser Tyr Glu 
                245                 250                 255     
Ala Met Asp Phe Val Arg Gln Leu Arg Glu Gln Gly Glu Ala Cys Tyr 
            260                 265                 270         
Phe Thr Met Asp Ala Gly Pro Asn Val Lys Val Leu Cys Gln Glu Lys 
        275                 280                 285             
Asp Leu Glu His Leu Ser Glu Ile Phe Gly Gln Arg Tyr Arg Leu Ile 
    290                 295                 300                 
Val Ser Lys Thr Lys Asp Leu Ser Gln Asp Gly Cys Cys 
305                 310                 315         

<210> 2
<211> 317
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutant 2B4 of MDP decarboxylase

<400> 2
Met Asp Arg Glu Pro Val Thr Val Arg Ser Tyr Ala Asn Ile Ala Ile 
1               5                   10                  15      
Ile Lys Tyr Trp Gly Lys Lys Lys Glu Lys Glu Met Val Pro Ala Thr 
            20                  25                  30          
Ser Ser Ile Ser Leu Thr Leu Glu Asn Met Tyr Thr Glu Thr Thr Leu 
        35                  40                  45              
Ser Ser Leu Pro Thr Asp Ala Thr Ala Asp Ala Phe Tyr Ile Asn Gly 
    50                  55                  60                  
Gln Leu Gln Asn Glu Ala Glu His Val Lys Met Ser Lys Ile Ile Asp 
65                  70                  75                  80  
Arg Tyr Arg Pro Asp Gly Asp Gly Phe Val Arg Ile Asp Thr Gln Asn 
                85                  90                  95      
Ser Met Pro Thr Ala Ala Gly Leu Ser Ser Ser Ser Ser Gly Leu Ser 
            100                 105                 110         
Ala Leu Val Lys Ala Cys Asn Ala Arg Phe Lys Leu Gly Leu Asn Arg 
        115                 120                 125             
Ser Gln Leu Ala Gln Glu Ala Lys Phe Ala Ser Gly Ser Ser Ser Arg 
    130                 135                 140                 
Ser Phe Tyr Gly Pro Leu Gly Ala Trp Asp Lys Asp Ser Gly Leu Ile 
145                 150                 155                 160 
Tyr Pro Val Glu Thr Gly Leu Lys Leu Ala Met Ile Cys Leu Val Leu 
                165                 170                 175     
Glu Asp Lys Lys Lys Pro Ile Ser Ser Arg Asp Gly Met Lys Leu Cys 
            180                 185                 190         
Val Glu Thr Ser Thr Thr Phe Asp Asp Trp Val Arg Gln Ser Glu Lys 
        195                 200                 205             
Asp Tyr Gln Asp Met Leu Val Tyr Leu Lys Ala Asn Asp Phe Ala Lys 
    210                 215                 220                 
Val Gly Glu Leu Thr Glu Lys Asn Ala Leu Ala Met His Ala Thr Thr 
225                 230                 235                 240 
Lys Thr Ala Ser Pro Ala Phe Ser Tyr Leu Thr Asp Ala Ser Tyr Glu 
                245                 250                 255     
Ala Met Asp Phe Val Arg Gln Leu Arg Glu Gln Gly Glu Ala Cys Tyr 
            260                 265                 270         
Phe Thr Met Asp Ala Gly Pro Asn Val Cys Val Leu Cys Gln Glu Lys 
        275                 280                 285             
Asp Leu Glu His Leu Ser Glu Ile Phe Gly Gln Arg Tyr Arg Met Ile 
    290                 295                 300                 
Val Ser Lys Ser Lys Asp Leu Ser Gln Asp Gly Cys Cys 
305                 310                 315         

<210> 3
<211> 317
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutant F9 of MDP decarboxylase

<400> 3
Met Asp Arg Glu Pro Val Thr Val Arg Ser Tyr Ala Asn Ile Ala Ile 
1               5                   10                  15      
Ile Lys Tyr Trp Gly Lys Lys Arg Glu Lys Glu Met Val Pro Ala Thr 
            20                  25                  30          
Ser Ser Ile Ser Leu Thr Leu Glu Asn Met Tyr Thr Glu Thr Thr Leu 
        35                  40                  45              
Ser Ser Leu Pro Thr Asp Ala Thr Ala Asp Ala Phe Tyr Ile Asn Gly 
    50                  55                  60                  
Gln Leu Gln Asn Glu Ala Glu His Val Lys Met Ser Lys Ile Ile Asp 
65                  70                  75                  80  
Arg Tyr Arg Pro Asp Gly Asp Gly Phe Val Arg Ile Asp Thr Gln Asn 
                85                  90                  95      
Ser Met Pro Thr Ala Ala Gly Leu Ser Ser Ser Ser Ser Gly Leu Ser 
            100                 105                 110         
Ala Leu Val Lys Ala Leu Asn Ala Arg Phe Lys Leu Gly Leu Asn Arg 
        115                 120                 125             
Ser Gln Leu Ala Gln Glu Ala Lys Phe Ala Ser Gly Ser Ser Ser Arg 
    130                 135                 140                 
Ser Phe Tyr Gly Pro Leu Gly Ala Trp Asp Lys Asp Ser Gly Leu Ile 
145                 150                 155                 160 
Tyr Pro Val Glu Thr Gly Leu Lys Leu Ala Met Ile Cys Leu Val Leu 
                165                 170                 175     
Cys Asp Lys Lys Lys Pro Ile Ser Ser Arg Asp Gly Met Lys Leu Cys 
            180                 185                 190         
Val Glu Thr Ser Thr Thr Phe Asp Asp Trp Val Arg Gln Ser Glu Lys 
        195                 200                 205             
Asp Tyr Gln Asp Met Leu Val Tyr Leu Lys Ala Asn Asp Phe Ala Lys 
    210                 215                 220                 
Val Gly Glu Leu Thr Glu Lys Asn Ala Leu Ala Met His Ala Thr Thr 
225                 230                 235                 240 
Lys Thr Ala Ser Pro Ala Phe Ser Tyr Leu Thr Asp Ala Ser Tyr Glu 
                245                 250                 255     
Ala Met Asp Phe Val Arg Gln Leu Arg Glu Gln Gly Glu Ala Cys Tyr 
            260                 265                 270         
Phe Thr Met Asp Ala Gly Pro Asn Val Cys Val Leu Cys Gln Glu Lys 
        275                 280                 285             
Asp Leu Asp His Leu Ser Glu Ile Leu Gly Gln Arg Tyr Arg Met Ile 
    290                 295                 300                 
Val Ser Lys Ser Lys Asp Leu Ser Gln Asp Gly Cys Cys 
305                 310                 315         

<210> 4
<211> 957
<212> DNA
<213> Streptococcus mitis

<220> 
<221> source
<222> 1..957
<223> /mol_type="unassigned DNA"
      /organism="Streptococcus mitis"

<400> 4
atggatcgtg aaccggttac cgttcgtagc tatgcaaata ttgccatcat caaatattgg      60

ggcaaaaaaa aagaaaaaga aatggttccg gcaaccagca gcattagcct gaccctggaa     120

aatatgtata ccgaaaccac cctgagcagc ctgccgaccg atgcaaccgc agatgcattt     180

tatattaatg gtcagctgca gaacgaagcc gaacatgtta aaatgagcaa aatcatcgat     240

cgctatcgtc cggatggtga tggttttgtt cgtattgata cccagaatag tatgccgacc     300

gcagcaggtc tgagcagcag cagcagtggt ctgagcgcac tggttaaagc atgtaatgcc     360

tattttaaac tgggtctgaa tcgtagccag ctggcacaag aagcaaaatt tgcaagcggt     420

agcagcagcc gtagctttta tggtccgctg ggtgcatggg ataaagatag cggtgaaatt     480

tatccggttg aaaccggtct gaaactggca atgattatgc tggttctgga agataaaaaa     540

aaaccgatta gcagccgtga tggtatgaaa ctgtgtgttg aaaccagcac cacctttgat     600

gattgggttc gtcagagcga aaaagattat caggatatgc tggtgtacct gaaagcaaat     660

gattttgcca aagttggtga gctgaccgaa aaaaatgcac tggcaatgca cgcaaccacc     720

aaaaccgcaa gtccggcatt tagctatctg accgatgcaa gctatgaagc aatggatttt     780

gttcgtcagc tgcgtgaaca gggtgaagca tgttacttta caatggatgc aggtccgaat     840

gttaaagttc tgtgccaaga aaaagacctg gaacatctga gcgaaatttt tggtcagcgt     900

tatcgtctga ttgtgagcaa aaccaaagat ctgagccagg atggttgctg ttaataa        957


