SEQUENCE LISTING

<110>   NOVARTIS AG
        CHILDRENS MEDICAL CENTER CORPORATION
        BRANDEIS UNIVERSITY

<120>   ROTAVIRUS PARTICLES WITH CHIMERIC SURFACE PROTEINS

<130>   P064203WO

<141>   2014-07-07

<160>   83

<170>   SeqWin2010, version 1.0

<210>   1
<211>   28
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   HIV HXB2 strain HR2 sequence

<400>   1
Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile His 
1               5                   10                  15      

Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys 
            20                  25              

<210>   2
<211>   34
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   HIV HXB2 HR1 sequence

<400>   2
Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala 
1               5                   10                  15      

Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln 
            20                  25                  30          

Ala Arg 
        

<210>   3
<211>   30
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Nipah virus HR2 sequence

<400>   3
Asp Ile Ser Ser Gln Ile Ser Ser Met Asn Gln Ser Leu Gln Gln Ser 
1               5                   10                  15      

Lys Asp Tyr Ile Lys Glu Ala Gln Arg Leu Leu Asp Thr Val 
            20                  25                  30  

<210>   4
<211>   32
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Nipah virus HR1 sequence

<400>   4
Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val Val Lys 
1               5                   10                  15      

Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala Leu Gln 
            20                  25                  30          

<210>   5
<211>   5
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Linker

<400>   5
Gly Gly Ser Gly Gly 
1               5   

<210>   6
<211>   8
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Linker

<400>   6
Gly Ser Gly Ser Gly Ser Gly Thr 
1               5               

<210>   7
<211>   5
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Linker

<400>   7
Glu Ala Ala Ala Arg 
1               5   

<210>   8
<211>   12
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Human factor XI recognition site

<400>   8
Gln Thr Ser Lys Leu Thr Arg Ala Glu Ala Val Phe 
1               5                   10          

<210>   9
<211>   12
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Human factor XI recognition site

<400>   9
Ser Phe Asn Asp Phe Thr Arg Val Val Gly Gly Glu 
1               5                   10          

<210>   10
<211>   13
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Human kallikrein recognition site

<400>   10
Leu Phe Ser Ser Met Thr Arg Val Val Gly Gly Leu Val 
1               5                   10              

<210>   11
<211>   10
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Human facor XII recognition site

<400>   11
Lys Ile Lys Pro Arg Ile Val Gly Gly Thr 
1               5                   10  

<210>   12
<211>   8
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   FLAG-tag

<400>   12
Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               

<210>   13
<211>   9
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   HA-tag

<400>   13
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   

<210>   14
<211>   6
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   His-tag

<400>   14
His His His His His His 
1               5       

<210>   15
<211>   10
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Myc-tag

<400>   15
Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  

<210>   16
<211>   9
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Strep-tag I

<400>   16
Ala Trp Arg His Pro Gln Phe Gly Gly 
1               5                   

<210>   17
<211>   9
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Strep-tag II

<400>   17
Asn Trp Ser His Pro Gln Phe Glu Lys 
1               5                   

<210>   18
<211>   12
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   protein C-tag

<400>   18
Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys 
1               5                   10          

<210>   19
<211>   6
<212>   DNA
<213>   Artificial Sequence

<220>
<223>   Kozak sequence

<400>   19
gccacc                                                             6

<210>   20
<211>   15
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Linker

<400>   20
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
1               5                   10                  15  

<210>   21
<211>   17
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Linker

<400>   21
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      

Gly 
    

<210>   22
<211>   367
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   22
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Ile Glu Gly Arg Ser Gly Gly Glu Asp Gln Val 
                165                 170                 175     

Asp Pro Arg Leu Ile Asp Gly Lys Ser Gly Gly Thr Leu Gln Leu Asp 
            180                 185                 190         

Met Ser Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro 
        195                 200                 205             

Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys 
    210                 215                 220                 

Trp Ile Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn 
225                 230                 235                 240 

Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe 
                245                 250                 255     

Glu Glu Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp 
            260                 265                 270         

Gly Val Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg 
        275                 280                 285             

Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val 
    290                 295                 300                 

Gly Gly Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro 
305                 310                 315                 320 

Gln Thr Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val 
                325                 330                 335     

Phe Tyr Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser 
            340                 345                 350         

Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360                 365         

<210>   23
<211>   365
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   23
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Ile Glu Gly Arg Ser Gly Gly Glu Asp Gln Val 
                165                 170                 175     

Asp Pro Arg Leu Ile Asp Gly Lys Gly Thr Leu Gln Leu Asp Met Ser 
            180                 185                 190         

Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp 
        195                 200                 205             

Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile 
    210                 215                 220                 

Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln 
225                 230                 235                 240 

Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu 
                245                 250                 255     

Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val 
            260                 265                 270         

Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys 
        275                 280                 285             

Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly 
    290                 295                 300                 

Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr 
305                 310                 315                 320 

Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr 
                325                 330                 335     

Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg 
            340                 345                 350         

Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360                 365 

<210>   24
<211>   363
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   24
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Ser Gly Gly Glu Asp Gln Val Asp Pro Arg Leu 
                165                 170                 175     

Ile Asp Gly Lys Ser Gly Gly Thr Leu Gln Leu Asp Met Ser Glu Leu 
            180                 185                 190         

Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr 
        195                 200                 205             

Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met 
    210                 215                 220                 

Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu 
225                 230                 235                 240 

Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala 
                245                 250                 255     

Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His 
            260                 265                 270         

Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys 
        275                 280                 285             

Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp 
    290                 295                 300                 

Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg 
305                 310                 315                 320 

Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val 
                325                 330                 335     

Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg 
            340                 345                 350         

Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360             

<210>   25
<211>   361
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   25
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Ser Gly Gly Glu Asp Gln Val Asp Pro Arg Leu 
                165                 170                 175     

Ile Asp Gly Lys Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp 
            180                 185                 190         

Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr 
        195                 200                 205             

Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser 
    210                 215                 220                 

Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile 
225                 230                 235                 240 

Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala 
                245                 250                 255     

Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu 
            260                 265                 270         

Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly 
        275                 280                 285             

Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu 
    290                 295                 300                 

Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met 
305                 310                 315                 320 

Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp 
                325                 330                 335     

Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu 
            340                 345                 350         

Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360     

<210>   26
<211>   359
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   26
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Gly Glu Asp Gln Val Asp Pro Arg Leu Ile Asp 
                165                 170                 175     

Gly Lys Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp Leu Ile 
            180                 185                 190         

Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr 
        195                 200                 205             

Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser Ser Cys 
    210                 215                 220                 

Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys 
225                 230                 235                 240 

Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala Glu Lys 
                245                 250                 255     

Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu Asp Val 
            260                 265                 270         

Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg 
        275                 280                 285             

Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu Asp Ile 
    290                 295                 300                 

Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met Arg Ile 
305                 310                 315                 320 

Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp Tyr Val 
                325                 330                 335     

Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu Asn Ser 
            340                 345                 350         

Ala Ala Phe Tyr Tyr Arg Ile 
        355                 

<210>   27
<211>   377
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   27
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp 
                165                 170                 175     

Ala Ser Leu Ser Gly Gly Glu Asp Gln Val Asp Pro Arg Leu Ile Asp 
            180                 185                 190         

Gly Lys Ser Gly Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp 
        195                 200                 205             

Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr 
    210                 215                 220                 

Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser 
225                 230                 235                 240 

Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile 
                245                 250                 255     

Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala 
            260                 265                 270         

Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu 
        275                 280                 285             

Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly 
    290                 295                 300                 

Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu 
305                 310                 315                 320 

Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met 
                325                 330                 335     

Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp 
            340                 345                 350         

Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu 
        355                 360                 365             

Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
    370                 375         

<210>   28
<211>   373
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   28
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp 
                165                 170                 175     

Ala Ser Leu Gly Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys 
            180                 185                 190         

Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn 
        195                 200                 205             

Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln 
    210                 215                 220                 

Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile 
225                 230                 235                 240 

Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr 
                245                 250                 255     

Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val 
            260                 265                 270         

Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu Asp Val Thr Thr 
        275                 280                 285             

Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn 
    290                 295                 300                 

Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala 
305                 310                 315                 320 

Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp 
                325                 330                 335     

Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln 
            340                 345                 350         

Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala 
        355                 360                 365             

Phe Tyr Tyr Arg Ile 
    370             

<210>   29
<211>   373
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   29
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Gly Leu Glu Leu Asp Lys Trp Ala Ser Leu Ser 
                165                 170                 175     

Gly Gly Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Ser Gly 
            180                 185                 190         

Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn 
        195                 200                 205             

Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln 
    210                 215                 220                 

Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile 
225                 230                 235                 240 

Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr 
                245                 250                 255     

Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val 
            260                 265                 270         

Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu Asp Val Thr Thr 
        275                 280                 285             

Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn 
    290                 295                 300                 

Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala 
305                 310                 315                 320 

Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp 
                325                 330                 335     

Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln 
            340                 345                 350         

Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala 
        355                 360                 365             

Phe Tyr Tyr Arg Ile 
    370             

<210>   30
<211>   369
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   30
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Gly Leu Glu Leu Asp Lys Trp Ala Ser Leu Gly 
                165                 170                 175     

Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Gly Thr Leu Gln 
            180                 185                 190         

Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys 
        195                 200                 205             

Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala 
    210                 215                 220                 

Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys Pro 
225                 230                 235                 240 

Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala 
                245                 250                 255     

Thr Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp Val 
            260                 265                 270         

Val Asp Gly Val Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys Thr 
        275                 280                 285             

Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val Ile 
    290                 295                 300                 

Gln Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr Thr 
305                 310                 315                 320 

Ala Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp Trp 
                325                 330                 335     

Gln Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln Ala 
            340                 345                 350         

Met Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg 
        355                 360                 365             

Ile 
    

<210>   31
<211>   379
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   31
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp Arg Glu 
    130                 135                 140                 

Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln 
145                 150                 155                 160 

Asn Gln Gln Glu Lys Gly Leu Glu Leu Asp Lys Trp Ala Ser Leu Gly 
                165                 170                 175     

Ser Ile Glu Gly Arg Ser Gly Gly Glu Asp Gln Val Asp Pro Arg Leu 
            180                 185                 190         

Ile Asp Gly Lys Ser Gly Gly Thr Leu Gln Leu Asp Met Ser Glu Leu 
        195                 200                 205             

Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr 
    210                 215                 220                 

Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met 
225                 230                 235                 240 

Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu 
                245                 250                 255     

Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala 
            260                 265                 270         

Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His 
        275                 280                 285             

Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys 
    290                 295                 300                 

Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp 
305                 310                 315                 320 

Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg 
                325                 330                 335     

Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val 
            340                 345                 350         

Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg 
        355                 360                 365             

Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
    370                 375                 

<210>   32
<211>   370
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   32
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Lys Ile Glu Gly Arg Ser Gly Gly Glu 
                165                 170                 175     

Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Ser Gly Gly Thr Leu 
            180                 185                 190         

Gln Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu 
        195                 200                 205             

Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu 
    210                 215                 220                 

Ala Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys 
225                 230                 235                 240 

Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr 
                245                 250                 255     

Ala Thr Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp 
            260                 265                 270         

Val Val Asp Gly Val Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys 
        275                 280                 285             

Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val 
    290                 295                 300                 

Ile Gln Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr 
305                 310                 315                 320 

Thr Ala Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp 
                325                 330                 335     

Trp Gln Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln 
            340                 345                 350         

Ala Met Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr 
        355                 360                 365             

Arg Ile 
    370 

<210>   33
<211>   368
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   33
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Lys Ile Glu Gly Arg Ser Gly Gly Glu 
                165                 170                 175     

Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Gly Thr Leu Gln Leu 
            180                 185                 190         

Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn 
        195                 200                 205             

Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn 
    210                 215                 220                 

Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu 
225                 230                 235                 240 

Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr 
                245                 250                 255     

Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val 
            260                 265                 270         

Asp Gly Val Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile 
        275                 280                 285             

Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln 
    290                 295                 300                 

Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala 
305                 310                 315                 320 

Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln 
                325                 330                 335     

Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met 
            340                 345                 350         

Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360                 365             

<210>   34
<211>   365
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   34
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Ser Gly Gly Glu Asp Gln Val Asp Pro 
                165                 170                 175     

Arg Leu Ile Asp Gly Lys Ser Gly Gly Thr Leu Gln Leu Asp Met Ser 
            180                 185                 190         

Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp 
        195                 200                 205             

Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile 
    210                 215                 220                 

Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln 
225                 230                 235                 240 

Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu 
                245                 250                 255     

Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val 
            260                 265                 270         

Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys 
        275                 280                 285             

Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly 
    290                 295                 300                 

Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr 
305                 310                 315                 320 

Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr 
                325                 330                 335     

Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg 
            340                 345                 350         

Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360                 365 

<210>   35
<211>   363
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   35
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Ser Gly Gly Glu Asp Gln Val Asp Pro 
                165                 170                 175     

Arg Leu Ile Asp Gly Lys Gly Thr Leu Gln Leu Asp Met Ser Glu Leu 
            180                 185                 190         

Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr 
        195                 200                 205             

Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met 
    210                 215                 220                 

Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu 
225                 230                 235                 240 

Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala 
                245                 250                 255     

Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His 
            260                 265                 270         

Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys 
        275                 280                 285             

Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp 
    290                 295                 300                 

Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg 
305                 310                 315                 320 

Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val 
                325                 330                 335     

Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg 
            340                 345                 350         

Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360             

<210>   36
<211>   361
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   36
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Gly Glu Asp Gln Val Asp Pro Arg Leu 
                165                 170                 175     

Ile Asp Gly Lys Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp 
            180                 185                 190         

Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr 
        195                 200                 205             

Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser 
    210                 215                 220                 

Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile 
225                 230                 235                 240 

Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala 
                245                 250                 255     

Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu 
            260                 265                 270         

Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly 
        275                 280                 285             

Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu 
    290                 295                 300                 

Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met 
305                 310                 315                 320 

Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp 
                325                 330                 335     

Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu 
            340                 345                 350         

Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360     

<210>   37
<211>   379
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   37
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Asn Glu Gln Glu Leu Leu Glu Leu Asp 
                165                 170                 175     

Lys Trp Ala Ser Leu Ser Gly Gly Glu Asp Gln Val Asp Pro Arg Leu 
            180                 185                 190         

Ile Asp Gly Lys Ser Gly Gly Thr Leu Gln Leu Asp Met Ser Glu Leu 
        195                 200                 205             

Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr 
    210                 215                 220                 

Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met 
225                 230                 235                 240 

Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu 
                245                 250                 255     

Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala 
            260                 265                 270         

Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His 
        275                 280                 285             

Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys 
    290                 295                 300                 

Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp 
305                 310                 315                 320 

Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg 
                325                 330                 335     

Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val 
            340                 345                 350         

Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg 
        355                 360                 365             

Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
    370                 375                 

<210>   38
<211>   376
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   38
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Lys Asn Glu Gln Glu Leu Leu Glu Leu 
                165                 170                 175     

Asp Lys Trp Ala Ser Leu Gly Glu Asp Gln Val Asp Pro Arg Leu Ile 
            180                 185                 190         

Asp Gly Lys Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp Leu 
        195                 200                 205             

Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr 
    210                 215                 220                 

Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser Ser 
225                 230                 235                 240 

Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly 
                245                 250                 255     

Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala Glu 
            260                 265                 270         

Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu Asp 
        275                 280                 285             

Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro 
    290                 295                 300                 

Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu Asp 
305                 310                 315                 320 

Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met Arg 
                325                 330                 335     

Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp Tyr 
            340                 345                 350         

Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu Asn 
        355                 360                 365             

Ser Ala Ala Phe Tyr Tyr Arg Ile 
    370                 375     

<210>   39
<211>   375
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   39
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Gly Leu Glu Leu Asp Lys Trp Ala Ser 
                165                 170                 175     

Leu Ser Gly Gly Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys 
            180                 185                 190         

Ser Gly Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp Leu Ile 
        195                 200                 205             

Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr 
    210                 215                 220                 

Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser Ser Cys 
225                 230                 235                 240 

Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys 
                245                 250                 255     

Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala Glu Lys 
            260                 265                 270         

Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu Asp Val 
        275                 280                 285             

Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg 
    290                 295                 300                 

Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu Asp Ile 
305                 310                 315                 320 

Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met Arg Ile 
                325                 330                 335     

Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp Tyr Val 
            340                 345                 350         

Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu Asn Ser 
        355                 360                 365             

Ala Ala Phe Tyr Tyr Arg Ile 
    370                 375 

<210>   40
<211>   371
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   40
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Gly Leu Glu Leu Asp Lys Trp Ala Ser 
                165                 170                 175     

Leu Gly Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Gly Thr 
            180                 185                 190         

Leu Gln Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp 
        195                 200                 205             

Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp 
    210                 215                 220                 

Glu Ala Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile Lys Val 
225                 230                 235                 240 

Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp 
                245                 250                 255     

Thr Ala Thr Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val Ile Thr 
            260                 265                 270         

Asp Val Val Asp Gly Val Asn His Lys Leu Asp Val Thr Thr Ala Thr 
        275                 280                 285             

Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn Val Ala 
    290                 295                 300                 

Val Ile Gln Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala Asp Pro 
305                 310                 315                 320 

Thr Thr Ala Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp Lys Lys 
                325                 330                 335     

Trp Trp Gln Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln Ile Ile 
            340                 345                 350         

Gln Ala Met Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr 
        355                 360                 365             

Tyr Arg Ile 
    370     

<210>   41
<211>   381
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   41
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp Ser Ala Trp Ser His Pro Gln Phe Glu 
            20                  25                  30          

Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn Leu Pro 
        35                  40                  45              

Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln Glu Glu 
    50                  55                  60                  

Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu Ala Ala 
65                  70                  75                  80  

Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln Leu Phe 
                85                  90                  95      

Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu Tyr Thr 
            100                 105                 110         

Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp Tyr Asn 
        115                 120                 125             

Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln Ile Ser 
    130                 135                 140                 

Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala 
145                 150                 155                 160 

Gln Arg Leu Leu Asp Thr Val Gly Leu Glu Leu Asp Lys Trp Ala Ser 
                165                 170                 175     

Leu Gly Ser Ile Glu Gly Arg Ser Gly Gly Glu Asp Gln Val Asp Pro 
            180                 185                 190         

Arg Leu Ile Asp Gly Lys Ser Gly Gly Thr Leu Gln Leu Asp Met Ser 
        195                 200                 205             

Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp 
    210                 215                 220                 

Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile 
225                 230                 235                 240 

Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln 
                245                 250                 255     

Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu 
            260                 265                 270         

Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val 
        275                 280                 285             

Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys 
    290                 295                 300                 

Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly 
305                 310                 315                 320 

Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr 
                325                 330                 335     

Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr 
            340                 345                 350         

Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg 
        355                 360                 365             

Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
    370                 375                 380     

<210>   42
<211>   369
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   42
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      

Ala Val Phe Val Ser Pro Ser Glu Asp Ser Ala Trp Ser His Pro Gln 
            20                  25                  30          

Phe Glu Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn 
        35                  40                  45              

Leu Pro Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln 
    50                  55                  60                  

Glu Glu Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu 
65                  70                  75                  80  

Ala Ala Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln 
                85                  90                  95      

Leu Phe Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu 
            100                 105                 110         

Tyr Thr Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp 
        115                 120                 125             

Tyr Asn Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp 
    130                 135                 140                 

Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu 
145                 150                 155                 160 

Ser Gln Asn Gln Gln Glu Lys Ile Glu Gly Arg Ser Gly Gly Glu Asp 
                165                 170                 175     

Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Ser Gly Gly Thr Leu Gln 
            180                 185                 190         

Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys 
        195                 200                 205             

Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala 
    210                 215                 220                 

Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile Lys Val Cys Pro 
225                 230                 235                 240 

Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala 
                245                 250                 255     

Thr Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val Ile Thr Asp Val 
            260                 265                 270         

Val Asp Gly Val Asn His Lys Leu Asp Val Thr Thr Ala Thr Cys Thr 
        275                 280                 285             

Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn Val Ala Val Ile 
    290                 295                 300                 

Gln Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala Asp Pro Thr Thr 
305                 310                 315                 320 

Ala Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp Lys Lys Trp Trp 
                325                 330                 335     

Gln Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln Ile Ile Gln Ala 
            340                 345                 350         

Met Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg 
        355                 360                 365             

Ile 
    

<210>   43
<211>   361
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   43
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      

Ala Val Phe Val Ser Pro Ser Glu Asp Ser Ala Trp Ser His Pro Gln 
            20                  25                  30          

Phe Glu Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn 
        35                  40                  45              

Leu Pro Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln 
    50                  55                  60                  

Glu Glu Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu 
65                  70                  75                  80  

Ala Ala Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln 
                85                  90                  95      

Leu Phe Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu 
            100                 105                 110         

Tyr Thr Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp 
        115                 120                 125             

Tyr Asn Val Val Leu Met Lys Tyr Asp Ala Gly Trp Met Glu Trp Asp 
    130                 135                 140                 

Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu 
145                 150                 155                 160 

Ser Gln Asn Gln Gln Glu Lys Gly Glu Asp Gln Val Asp Pro Arg Leu 
                165                 170                 175     

Ile Asp Gly Lys Gly Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp 
            180                 185                 190         

Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr 
        195                 200                 205             

Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser 
    210                 215                 220                 

Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile 
225                 230                 235                 240 

Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala 
                245                 250                 255     

Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His Lys Leu 
            260                 265                 270         

Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly 
        275                 280                 285             

Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu 
    290                 295                 300                 

Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met 
305                 310                 315                 320 

Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp 
                325                 330                 335     

Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu 
            340                 345                 350         

Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360     

<210>   44
<211>   372
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   44
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      

Ala Val Phe Val Ser Pro Ser Glu Asp Ser Ala Trp Ser His Pro Gln 
            20                  25                  30          

Phe Glu Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn 
        35                  40                  45              

Leu Pro Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln 
    50                  55                  60                  

Glu Glu Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu 
65                  70                  75                  80  

Ala Ala Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln 
                85                  90                  95      

Leu Phe Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu 
            100                 105                 110         

Tyr Thr Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp 
        115                 120                 125             

Tyr Asn Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln 
    130                 135                 140                 

Ile Ser Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys 
145                 150                 155                 160 

Glu Ala Gln Arg Leu Leu Asp Thr Val Lys Ile Glu Gly Arg Ser Gly 
                165                 170                 175     

Gly Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Ser Gly Gly 
            180                 185                 190         

Thr Leu Gln Leu Asp Met Ser Glu Leu Ala Asp Leu Ile Leu Asn Glu 
        195                 200                 205             

Trp Leu Cys Asn Pro Met Asp Ile Thr Leu Tyr Tyr Tyr Gln Gln Thr 
    210                 215                 220                 

Asp Glu Ala Asn Lys Trp Ile Ser Met Gly Ser Ser Cys Thr Ile Lys 
225                 230                 235                 240 

Val Cys Pro Leu Asn Thr Gln Thr Leu Gly Ile Gly Cys Leu Thr Thr 
                245                 250                 255     

Asp Thr Ala Thr Phe Glu Glu Val Ala Thr Ala Glu Lys Leu Val Ile 
            260                 265                 270         

Thr Asp Val Val Asp Gly Val Asn His Lys Leu Asp Val Thr Thr Ala 
        275                 280                 285             

Thr Cys Thr Ile Arg Asn Cys Lys Lys Leu Gly Pro Arg Glu Asn Val 
    290                 295                 300                 

Ala Val Ile Gln Val Gly Gly Ser Asp Val Leu Asp Ile Thr Ala Asp 
305                 310                 315                 320 

Pro Thr Thr Ala Pro Gln Thr Glu Arg Met Met Arg Ile Asn Trp Lys 
                325                 330                 335     

Lys Trp Trp Gln Val Phe Tyr Thr Val Val Asp Tyr Val Asn Gln Ile 
            340                 345                 350         

Ile Gln Ala Met Ser Lys Arg Ser Arg Ser Leu Asn Ser Ala Ala Phe 
        355                 360                 365             

Tyr Tyr Arg Ile 
    370         

<210>   45
<211>   363
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2Nipah fusion protein

<400>   45
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      

Ala Val Phe Val Ser Pro Ser Glu Asp Ser Ala Trp Ser His Pro Gln 
            20                  25                  30          

Phe Glu Lys Glu Asn Leu Tyr Phe Gln Gly Gln Asn Tyr Gly Ile Asn 
        35                  40                  45              

Leu Pro Ile Thr Gly Ser Met Asp Thr Ala Tyr Ala Asn Ser Thr Gln 
    50                  55                  60                  

Glu Glu Thr Phe Leu Thr Ser Thr Leu Cys Leu Tyr Tyr Pro Thr Glu 
65                  70                  75                  80  

Ala Ala Thr Glu Ile Asn Asp Asn Ser Trp Lys Asp Thr Leu Ser Gln 
                85                  90                  95      

Leu Phe Leu Thr Lys Gly Trp Pro Thr Gly Ser Val Tyr Phe Lys Glu 
            100                 105                 110         

Tyr Thr Asp Ile Ala Ser Phe Ser Val Asp Pro Gln Leu Tyr Cys Asp 
        115                 120                 125             

Tyr Asn Val Val Leu Met Lys Tyr Asp Ala Gly Asp Ile Ser Ser Gln 
    130                 135                 140                 

Ile Ser Ser Met Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys 
145                 150                 155                 160 

Glu Ala Gln Arg Leu Leu Asp Thr Val Gly Glu Asp Gln Val Asp Pro 
                165                 170                 175     

Arg Leu Ile Asp Gly Lys Gly Thr Leu Gln Leu Asp Met Ser Glu Leu 
            180                 185                 190         

Ala Asp Leu Ile Leu Asn Glu Trp Leu Cys Asn Pro Met Asp Ile Thr 
        195                 200                 205             

Leu Tyr Tyr Tyr Gln Gln Thr Asp Glu Ala Asn Lys Trp Ile Ser Met 
    210                 215                 220                 

Gly Ser Ser Cys Thr Ile Lys Val Cys Pro Leu Asn Thr Gln Thr Leu 
225                 230                 235                 240 

Gly Ile Gly Cys Leu Thr Thr Asp Thr Ala Thr Phe Glu Glu Val Ala 
                245                 250                 255     

Thr Ala Glu Lys Leu Val Ile Thr Asp Val Val Asp Gly Val Asn His 
            260                 265                 270         

Lys Leu Asp Val Thr Thr Ala Thr Cys Thr Ile Arg Asn Cys Lys Lys 
        275                 280                 285             

Leu Gly Pro Arg Glu Asn Val Ala Val Ile Gln Val Gly Gly Ser Asp 
    290                 295                 300                 

Val Leu Asp Ile Thr Ala Asp Pro Thr Thr Ala Pro Gln Thr Glu Arg 
305                 310                 315                 320 

Met Met Arg Ile Asn Trp Lys Lys Trp Trp Gln Val Phe Tyr Thr Val 
                325                 330                 335     

Val Asp Tyr Val Asn Gln Ile Ile Gln Ala Met Ser Lys Arg Ser Arg 
            340                 345                 350         

Ser Leu Asn Ser Ala Ala Phe Tyr Tyr Arg Ile 
        355                 360             

<210>   46
<211>   629
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   46
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Arg Ser Leu Gln Glu Ala Gly Tyr Ile Pro Glu Ala Pro Arg 
                565                 570                 575     

Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
            580                 585                 590         

Thr Phe Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala 
        595                 600                 605             

Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys 
    610                 615                 620                 

Gln Leu Gln Ala Arg 
625                 

<210>   47
<211>   627
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1Nipah fusion protein

<400>   47
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Arg Ser Leu Gln Glu Ala Gly Tyr Ile Pro Glu Ala Pro Arg 
                565                 570                 575     

Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
            580                 585                 590         

Thr Asn Ile Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala 
        595                 600                 605             

Val Val Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr 
    610                 615                 620                 

Ala Leu Gln 
625         

<210>   48
<211>   596
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   48
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile 
                565                 570                 575     

Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
            580                 585                 590         

Leu Gln Ala Arg 
        595     

<210>   49
<211>   598
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   49
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Arg Ser Asn Ile Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr 
                565                 570                 575     

Asn Glu Ala Val Val Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr 
            580                 585                 590         

Val Leu Thr Ala Leu Gln 
        595             

<210>   50
<211>   595
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   50
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser 
    370                 375                 380                 

Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr 
385                 390                 395                 400 

Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly 
                405                 410                 415     

Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn Gly 
            420                 425                 430         

Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe 
        435                 440                 445             

Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu Asn 
    450                 455                 460                 

Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr Asn 
465                 470                 475                 480 

Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe His 
                485                 490                 495     

Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu Lys 
            500                 505                 510         

Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys 
        515                 520                 525             

Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr 
    530                 535                 540                 

Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp Gly 
545                 550                 555                 560 

Val Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu 
                565                 570                 575     

Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu 
            580                 585                 590         

Gln Ala Arg 
        595 

<210>   51
<211>   597
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1Nipah fusion protein

<400>   51
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser 
    370                 375                 380                 

Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr 
385                 390                 395                 400 

Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly 
                405                 410                 415     

Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn Gly 
            420                 425                 430         

Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe 
        435                 440                 445             

Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu Asn 
    450                 455                 460                 

Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr Asn 
465                 470                 475                 480 

Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe His 
                485                 490                 495     

Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu Lys 
            500                 505                 510         

Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys 
        515                 520                 525             

Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr 
    530                 535                 540                 

Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp Gly 
545                 550                 555                 560 

Val Arg Ser Asn Ile Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn 
                565                 570                 575     

Glu Ala Val Val Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val 
            580                 585                 590         

Leu Thr Ala Leu Gln 
        595         

<210>   52
<211>   595
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   52
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Ile Glu Gly 
    370                 375                 380                 

Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr 
385                 390                 395                 400 

Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly 
                405                 410                 415     

Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn Gly 
            420                 425                 430         

Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe 
        435                 440                 445             

Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu Asn 
    450                 455                 460                 

Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr Asn 
465                 470                 475                 480 

Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe His 
                485                 490                 495     

Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu Lys 
            500                 505                 510         

Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys 
        515                 520                 525             

Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr 
    530                 535                 540                 

Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp Gly 
545                 550                 555                 560 

Val Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu 
                565                 570                 575     

Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu 
            580                 585                 590         

Gln Ala Arg 
        595 

<210>   53
<211>   597
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1Nipah fusion protein

<400>   53
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Ile Glu Gly 
    370                 375                 380                 

Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr 
385                 390                 395                 400 

Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly 
                405                 410                 415     

Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn Gly 
            420                 425                 430         

Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe 
        435                 440                 445             

Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu Asn 
    450                 455                 460                 

Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr Asn 
465                 470                 475                 480 

Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe His 
                485                 490                 495     

Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu Lys 
            500                 505                 510         

Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys 
        515                 520                 525             

Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr 
    530                 535                 540                 

Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp Gly 
545                 550                 555                 560 

Val Arg Ser Asn Ile Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn 
                565                 570                 575     

Glu Ala Val Val Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val 
            580                 585                 590         

Leu Thr Ala Leu Gln 
        595         

<210>   54
<211>   596
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   54
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Ile Gly Glu Ala Arg Gln 
545                 550                 555                 560 

Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile 
                565                 570                 575     

Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
            580                 585                 590         

Leu Gln Ala Arg 
        595     

<210>   55
<211>   594
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1Nipah fusion protein

<400>   55
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Ile Gly Glu Ala Arg Gln 
545                 550                 555                 560 

Asn Ile Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val 
                565                 570                 575     

Val Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala 
            580                 585                 590         

Leu Gln 
        

<210>   56
<211>   603
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   56
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Arg Ser Ile Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln 
                565                 570                 575     

Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu 
            580                 585                 590         

Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
        595                 600             

<210>   57
<211>   601
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1Nipah fusion protein

<400>   57
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Arg Ser Ile Arg Gln Asn Ile Asn Lys Leu Lys Ser Ser Ile 
                565                 570                 575     

Glu Ser Thr Asn Glu Ala Val Val Lys Leu Gln Glu Thr Ala Glu Lys 
            580                 585                 590         

Thr Val Tyr Val Leu Thr Ala Leu Gln 
        595                 600     

<210>   58
<211>   610
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1HXB2 fusion protein

<400>   58
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Arg Ser Ile Lys Lys Leu Ile Gly Glu Ala Arg Gln Leu Leu 
                565                 570                 575     

Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala 
            580                 585                 590         

Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln 
        595                 600                 605             

Ala Arg 
    610 

<210>   59
<211>   608
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H1 Solomon Islands 2006) ectodomain-HR1Nipah fusion protein

<400>   59
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Thr Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Ser His 
                85                  90                  95      

Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile Ala Pro Leu Gln Leu Gly 
            100                 105                 110         

Asn Cys Ser Val Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Leu 
        115                 120                 125             

Leu Ile Ser Arg Glu Ser Trp Ser Tyr Ile Val Glu Lys Pro Asn Pro 
    130                 135                 140                 

Glu Asn Gly Thr Cys Tyr Pro Gly His Phe Ala Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
                165                 170                 175     

Pro Lys Glu Ser Ser Trp Pro Asn His Thr Thr Thr Gly Val Ser Ala 
            180                 185                 190         

Ser Cys Ser His Asn Gly Glu Ser Ser Phe Tyr Lys Asn Leu Leu Trp 
        195                 200                 205             

Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu Ser Lys Ser Tyr Ala 
    210                 215                 220                 

Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp Gly Val His His Pro 
225                 230                 235                 240 

Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr His Thr Glu Asn Ala Tyr 
                245                 250                 255     

Val Ser Val Val Ser Ser His Tyr Ser Arg Lys Phe Thr Pro Glu Ile 
            260                 265                 270         

Ala Lys Arg Pro Lys Val Arg Asp Arg Glu Gly Arg Ile Asn Tyr Tyr 
        275                 280                 285             

Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala Asn Gly 
    290                 295                 300                 

Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly Phe Gly 
305                 310                 315                 320 

Ser Gly Ile Ile Asn Ser Asn Ala Pro Met Asp Glu Cys Asp Ala Lys 
                325                 330                 335     

Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln Asn 
            340                 345                 350         

Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser Ala 
        355                 360                 365             

Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Asp Asp Asp 
    370                 375                 380                 

Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
385                 390                 395                 400 

Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln 
                405                 410                 415     

Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn 
            420                 425                 430         

Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln 
        435                 440                 445             

Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu 
    450                 455                 460                 

Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile Asp Ile Trp Thr Tyr 
465                 470                 475                 480 

Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe 
                485                 490                 495     

His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu 
            500                 505                 510         

Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His 
        515                 520                 525             

Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp 
    530                 535                 540                 

Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp 
545                 550                 555                 560 

Gly Val Arg Ser Ile Lys Lys Leu Ile Gly Glu Ala Arg Gln Asn Ile 
                565                 570                 575     

Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val Val Lys 
            580                 585                 590         

Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala Leu Gln 
        595                 600                 605             

<210>   60
<211>   606
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H5 Vietnam 2004) ectodomain-HR1HXB2 fusion protein

<400>   60
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Gln Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Lys His 
                85                  90                  95      

Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu Ile Leu Arg 
            100                 105                 110         

Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu 
        115                 120                 125             

Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro 
    130                 135                 140                 

Val Asn Asp Leu Cys Tyr Pro Gly Asp Phe Asn Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile 
                165                 170                 175     

Pro Lys Ser Ser Trp Ser Ser His Glu Ala Ser Leu Gly Val Ser Ser 
            180                 185                 190         

Ala Cys Pro Tyr Gln Gly Lys Ser Ser Phe Phe Arg Asn Val Val Trp 
        195                 200                 205             

Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn 
    210                 215                 220                 

Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro 
225                 230                 235                 240 

Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr 
                245                 250                 255     

Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Arg Ile 
            260                 265                 270         

Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe 
        275                 280                 285             

Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly 
    290                 295                 300                 

Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp 
305                 310                 315                 320 

Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly Asn Cys Asn Thr Lys 
                325                 330                 335     

Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn 
            340                 345                 350         

Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn 
        355                 360                 365             

Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Gln Arg Glu Arg 
    370                 375                 380                 

Asp Asp Asp Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu 
385                 390                 395                 400 

Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser 
                405                 410                 415     

Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys 
            420                 425                 430         

Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met 
        435                 440                 445             

Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg 
    450                 455                 460                 

Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val 
465                 470                 475                 480 

Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr 
                485                 490                 495     

Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg 
            500                 505                 510         

Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu 
        515                 520                 525             

Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn Gly 
    530                 535                 540                 

Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu 
545                 550                 555                 560 

Glu Ile Ser Gly Val Arg Ser Leu Arg Gln Leu Leu Ser Gly Ile Val 
                565                 570                 575     

Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu 
            580                 585                 590         

Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
        595                 600                 605     

<210>   61
<211>   632
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H5 Vietnam 2004) ectodomain-HR1HXB2 fusion protein

<400>   61
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Ala Asp Pro His His His His His His Glu 
        35                  40                  45              

Asn Leu Tyr Phe Gln Gly Gly Tyr Leu Leu Glu Asp Gln Ile Cys Ile 
    50                  55                  60                  

Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu 
65                  70                  75                  80  

Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Lys His 
                85                  90                  95      

Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu Ile Leu Arg 
            100                 105                 110         

Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu 
        115                 120                 125             

Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro 
    130                 135                 140                 

Val Asn Asp Leu Cys Tyr Pro Gly Asp Phe Asn Asp Tyr Glu Glu Leu 
145                 150                 155                 160 

Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile 
                165                 170                 175     

Pro Lys Ser Ser Trp Ser Ser His Glu Ala Ser Leu Gly Val Ser Ser 
            180                 185                 190         

Ala Cys Pro Tyr Gln Gly Lys Ser Ser Phe Phe Arg Asn Val Val Trp 
        195                 200                 205             

Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn 
    210                 215                 220                 

Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro 
225                 230                 235                 240 

Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr 
                245                 250                 255     

Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Arg Ile 
            260                 265                 270         

Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe 
        275                 280                 285             

Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly 
    290                 295                 300                 

Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp 
305                 310                 315                 320 

Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly Asn Cys Asn Thr Lys 
                325                 330                 335     

Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn 
            340                 345                 350         

Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn 
        355                 360                 365             

Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Gln Arg Glu Arg 
    370                 375                 380                 

Asp Asp Asp Asp Lys Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu 
385                 390                 395                 400 

Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser 
                405                 410                 415     

Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys 
            420                 425                 430         

Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met 
        435                 440                 445             

Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg 
    450                 455                 460                 

Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val 
465                 470                 475                 480 

Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr 
                485                 490                 495     

Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg 
            500                 505                 510         

Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu 
        515                 520                 525             

Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn Gly 
    530                 535                 540                 

Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu 
545                 550                 555                 560 

Glu Ile Ser Gly Val Arg Ser Leu Gln Glu Ala Gly Tyr Ile Pro Glu 
                565                 570                 575     

Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val 
            580                 585                 590         

Leu Leu Ser Thr Phe Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu 
        595                 600                 605             

Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp 
    610                 615                 620                 

Gly Ile Lys Gln Leu Gln Ala Arg 
625                 630         

<210>   62
<211>   602
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H3 Wisconsin 2005) ectodomain-HR1HXB2 fusion protein

<400>   62
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Gln Lys Leu Pro His His His His His His 
        35                  40                  45              

Glu Asn Leu Tyr Phe Gln Gly Gly Asn Asp Asn Ser Thr Ala Thr Leu 
    50                  55                  60                  

Cys Leu Gly His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile 
65                  70                  75                  80  

Thr Asn Asp Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser 
                85                  90                  95      

Ser Ser Thr Gly Gly Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly 
            100                 105                 110         

Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp 
        115                 120                 125             

Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala 
    130                 135                 140                 

Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg 
145                 150                 155                 160 

Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe Asn Asp Glu Ser Phe 
                165                 170                 175     

Asn Trp Thr Gly Val Thr Gln Asn Gly Thr Ser Ser Ser Cys Lys Arg 
            180                 185                 190         

Arg Ser Asn Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr Gln Leu 
        195                 200                 205             

Lys Phe Lys Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Lys 
    210                 215                 220                 

Phe Asp Lys Leu Tyr Ile Trp Gly Val His His Pro Val Thr Asp Asn 
225                 230                 235                 240 

Asp Gln Ile Phe Leu Tyr Ala Gln Ala Ser Gly Arg Ile Thr Val Ser 
                245                 250                 255     

Thr Lys Arg Ser Gln Gln Thr Val Ile Pro Asn Ile Gly Ser Arg Pro 
            260                 265                 270         

Arg Ile Arg Asn Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val 
        275                 280                 285             

Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala 
    290                 295                 300                 

Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg 
305                 310                 315                 320 

Ser Asp Ala Pro Ile Gly Lys Cys Asn Ser Glu Cys Ile Thr Pro Asn 
                325                 330                 335     

Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr 
            340                 345                 350         

Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala 
        355                 360                 365             

Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr Arg Asp Asp Asp Asp 
    370                 375                 380                 

Lys Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
385                 390                 395                 400 

Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
                405                 410                 415     

Ile Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asn Gln 
            420                 425                 430         

Ile Asn Gly Lys Leu Asn Arg Leu Ile Gly Lys Thr Asn Glu Lys Phe 
        435                 440                 445             

His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
    450                 455                 460                 

Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
465                 470                 475                 480 

Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
                485                 490                 495     

Asp Ser Glu Met Asn Lys Leu Phe Glu Arg Thr Lys Lys Gln Leu Arg 
            500                 505                 510         

Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
        515                 520                 525             

Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
    530                 535                 540                 

Asp Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
545                 550                 555                 560 

Val Arg Ser Leu Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn 
                565                 570                 575     

Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr 
            580                 585                 590         

Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
        595                 600         

<210>   63
<211>   628
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered influenza virus hemagglutinin (H3 Wisconsin 2005) ectodomain-HR1HXB2 fusion protein

<400>   63
Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      

Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          

Ala His Ser Ala Phe Ala Gln Lys Leu Pro His His His His His His 
        35                  40                  45              

Glu Asn Leu Tyr Phe Gln Gly Gly Asn Asp Asn Ser Thr Ala Thr Leu 
    50                  55                  60                  

Cys Leu Gly His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile 
65                  70                  75                  80  

Thr Asn Asp Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser 
                85                  90                  95      

Ser Ser Thr Gly Gly Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly 
            100                 105                 110         

Glu Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp 
        115                 120                 125             

Gly Phe Gln Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala 
    130                 135                 140                 

Tyr Ser Asn Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg 
145                 150                 155                 160 

Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe Asn Asp Glu Ser Phe 
                165                 170                 175     

Asn Trp Thr Gly Val Thr Gln Asn Gly Thr Ser Ser Ser Cys Lys Arg 
            180                 185                 190         

Arg Ser Asn Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr Gln Leu 
        195                 200                 205             

Lys Phe Lys Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Lys 
    210                 215                 220                 

Phe Asp Lys Leu Tyr Ile Trp Gly Val His His Pro Val Thr Asp Asn 
225                 230                 235                 240 

Asp Gln Ile Phe Leu Tyr Ala Gln Ala Ser Gly Arg Ile Thr Val Ser 
                245                 250                 255     

Thr Lys Arg Ser Gln Gln Thr Val Ile Pro Asn Ile Gly Ser Arg Pro 
            260                 265                 270         

Arg Ile Arg Asn Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val 
        275                 280                 285             

Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala 
    290                 295                 300                 

Pro Arg Gly Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg 
305                 310                 315                 320 

Ser Asp Ala Pro Ile Gly Lys Cys Asn Ser Glu Cys Ile Thr Pro Asn 
                325                 330                 335     

Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr 
            340                 345                 350         

Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala 
        355                 360                 365             

Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr Arg Asp Asp Asp Asp 
    370                 375                 380                 

Lys Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu 
385                 390                 395                 400 

Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly 
                405                 410                 415     

Ile Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asn Gln 
            420                 425                 430         

Ile Asn Gly Lys Leu Asn Arg Leu Ile Gly Lys Thr Asn Glu Lys Phe 
        435                 440                 445             

His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp 
    450                 455                 460                 

Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn 
465                 470                 475                 480 

Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr 
                485                 490                 495     

Asp Ser Glu Met Asn Lys Leu Phe Glu Arg Thr Lys Lys Gln Leu Arg 
            500                 505                 510         

Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys 
        515                 520                 525             

Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His 
    530                 535                 540                 

Asp Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly 
545                 550                 555                 560 

Val Arg Ser Leu Gln Glu Ala Gly Tyr Ile Pro Glu Ala Pro Arg Asp 
                565                 570                 575     

Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr 
            580                 585                 590         

Phe Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile 
        595                 600                 605             

Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
    610                 615                 620                 

Leu Gln Ala Arg 
625             

<210>   64
<211>   755
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   64
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val 
        35                  40                  45              

Pro Val Trp Lys Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala 
    50                  55                  60                  

Lys Ala Tyr Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys 
65                  70                  75                  80  

Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Tyr Met Glu Asn Val Thr 
                85                  90                  95      

Glu Glu Phe Asn Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr 
            100                 105                 110         

Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Gln Leu 
        115                 120                 125             

Thr Pro Leu Cys Val Thr Leu Asp Cys Ser Tyr Asn Ile Thr Asn Asn 
    130                 135                 140                 

Ile Thr Asn Ser Ile Thr Asn Ser Ser Val Asn Met Arg Glu Glu Ile 
145                 150                 155                 160 

Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg Asp Lys Asn Arg 
                165                 170                 175     

Lys Val Tyr Ser Leu Phe Tyr Lys Leu Asp Val Val Gln Ile Asn Asn 
            180                 185                 190         

Gly Asn Asn Ser Ser Asn Leu Tyr Arg Leu Ile Asn Cys Asn Thr Ser 
        195                 200                 205             

Ala Leu Thr Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile 
    210                 215                 220                 

Arg Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asp Lys 
225                 230                 235                 240 

Glu Phe Asn Gly Thr Gly Leu Cys Lys Asn Val Ser Thr Val Gln Cys 
                245                 250                 255     

Thr His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly 
            260                 265                 270         

Ser Leu Ala Glu Gly Lys Val Met Ile Arg Ser Glu Asn Ile Thr Asn 
        275                 280                 285             

Asn Val Lys Asn Ile Ile Val Gln Leu Asn Glu Thr Val Thr Ile Asn 
    290                 295                 300                 

Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Val Arg Ile Gly Pro 
305                 310                 315                 320 

Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln 
                325                 330                 335     

Ala His Cys Asn Val Ser Gly Ser Gln Trp Asn Arg Ala Leu His Gln 
            340                 345                 350         

Val Val Gly Gln Leu Arg Glu Tyr Trp Asn Thr Thr Ile Ile Phe Lys 
        355                 360                 365             

Asn Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys 
    370                 375                 380                 

Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Asn 
385                 390                 395                 400 

Trp Thr His Asn Asp Thr Ala Ser Met Lys Pro Asn Asp Thr Ile Thr 
                405                 410                 415     

Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Val Gly 
            420                 425                 430         

Gln Ala Ile Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Glu Ser 
        435                 440                 445             

Asn Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Gly Asn Ile Asn 
    450                 455                 460                 

Glu Ser Gln Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp 
465                 470                 475                 480 

Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu Pro Leu Gly 
                485                 490                 495     

Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg 
            500                 505                 510         

Ala Val Val Glu Leu Gly Ala Val Phe Ile Gly Phe Leu Gly Thr Ala 
        515                 520                 525             

Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Val Arg 
    530                 535                 540                 

Lys Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
545                 550                 555                 560 

Ile Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
                565                 570                 575     

Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln 
            580                 585                 590         

Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
        595                 600                 605             

Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Glu Arg Glu Ile 
    610                 615                 620                 

Trp Glu Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
625                 630                 635                 640 

Thr His Ile Ile Tyr Glu Leu Ile Glu Glu Ser Gln Lys Gln Gln Glu 
                645                 650                 655     

Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Asn Leu Trp 
            660                 665                 670         

Asn Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ser Arg Ile 
        675                 680                 685             

Glu Gly Arg Gly Ser Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly 
    690                 695                 700                 

Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe 
705                 710                 715                 720 

Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu 
                725                 730                 735     

Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu 
            740                 745                 750         

Gln Ala Arg 
        755 

<210>   65
<211>   763
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   65
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala Glu Thr 
    50                  55                  60                  

Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu Val His 
65                  70                  75                  80  

Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
                85                  90                  95      

Glu Ile Tyr Met Glu Asn Val Thr Glu Glu Phe Asn Met Trp Lys Asn 
            100                 105                 110         

Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser Leu Trp Asp Gln 
        115                 120                 125             

Ser Leu Lys Pro Cys Val Gln Leu Thr Pro Leu Cys Val Thr Leu Asp 
    130                 135                 140                 

Cys Ser Tyr Asn Ile Thr Asn Asn Ile Thr Asn Ser Ile Thr Asn Ser 
145                 150                 155                 160 

Ser Val Asn Met Arg Glu Glu Ile Lys Asn Cys Ser Phe Asn Met Thr 
                165                 170                 175     

Thr Glu Leu Arg Asp Lys Asn Arg Lys Val Tyr Ser Leu Phe Tyr Lys 
            180                 185                 190         

Leu Asp Val Val Gln Ile Asn Asn Gly Asn Asn Ser Ser Asn Leu Tyr 
        195                 200                 205             

Arg Leu Ile Asn Cys Asn Thr Ser Ala Leu Thr Gln Ala Cys Pro Lys 
    210                 215                 220                 

Val Thr Phe Glu Pro Ile Pro Ile Arg Tyr Cys Ala Pro Ala Gly Tyr 
225                 230                 235                 240 

Ala Ile Leu Lys Cys Asn Asp Lys Glu Phe Asn Gly Thr Gly Leu Cys 
                245                 250                 255     

Lys Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val 
            260                 265                 270         

Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Gly Lys Val Met 
        275                 280                 285             

Ile Arg Ser Glu Asn Ile Thr Asn Asn Val Lys Asn Ile Ile Val Gln 
    290                 295                 300                 

Leu Asn Glu Thr Val Thr Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr 
305                 310                 315                 320 

Arg Lys Ser Val Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly 
                325                 330                 335     

Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Gly Ser 
            340                 345                 350         

Gln Trp Asn Arg Ala Leu His Gln Val Val Gly Gln Leu Arg Glu Tyr 
        355                 360                 365             

Trp Asn Thr Thr Ile Ile Phe Lys Asn Ser Ser Gly Gly Asp Leu Glu 
    370                 375                 380                 

Ile Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn 
385                 390                 395                 400 

Thr Ser Gly Leu Phe Asn Ser Asn Trp Thr His Asn Asp Thr Ala Ser 
                405                 410                 415     

Met Lys Pro Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile 
            420                 425                 430         

Ile Asn Met Trp Gln Arg Val Gly Gln Ala Ile Tyr Ala Pro Pro Ile 
        435                 440                 445             

Gln Gly Val Ile Arg Cys Glu Ser Asn Ile Thr Gly Leu Ile Leu Thr 
    450                 455                 460                 

Arg Asp Gly Gly Gly Asn Ile Asn Glu Ser Gln Ile Phe Arg Pro Gly 
465                 470                 475                 480 

Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys 
                485                 490                 495     

Val Val Arg Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg 
            500                 505                 510         

Arg Val Val Glu Arg Glu Lys Arg Ala Val Val Glu Leu Gly Ala Val 
        515                 520                 525             

Phe Ile Gly Phe Leu Gly Thr Ala Gly Ser Thr Met Gly Ala Ala Ser 
    530                 535                 540                 

Ile Thr Leu Thr Val Gln Val Arg Lys Leu Leu Ser Gly Ile Val Gln 
545                 550                 555                 560 

Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu 
                565                 570                 575     

Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala 
            580                 585                 590         

Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys 
        595                 600                 605             

Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp 
    610                 615                 620                 

Ser Asn Lys Ser Glu Arg Glu Ile Trp Glu Asn Met Thr Trp Leu Gln 
625                 630                 635                 640 

Trp Asp Lys Glu Ile Ser Asn Tyr Thr His Ile Ile Tyr Glu Leu Ile 
                645                 650                 655     

Glu Glu Ser Gln Lys Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu 
            660                 665                 670         

Leu Asp Lys Trp Ala Asn Leu Trp Asn Trp Phe Asp Ile Ser Asn Trp 
        675                 680                 685             

Leu Trp Tyr Ile Lys Ser Arg Ile Glu Gly Arg Gly Ser Gly Gly Tyr 
    690                 695                 700                 

Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly 
705                 710                 715                 720 

Glu Trp Val Leu Leu Ser Thr Phe Leu Ser Gly Ile Val Gln Gln Gln 
                725                 730                 735     

Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu 
            740                 745                 750         

Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
        755                 760             

<210>   66
<211>   722
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   66
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val 
        35                  40                  45              

Pro Val Trp Lys Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala 
    50                  55                  60                  

Lys Ala Tyr Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys 
65                  70                  75                  80  

Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Tyr Met Glu Asn Val Thr 
                85                  90                  95      

Glu Glu Phe Asn Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr 
            100                 105                 110         

Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Gln Leu 
        115                 120                 125             

Thr Pro Leu Cys Val Thr Leu Asp Cys Ser Tyr Asn Ile Thr Asn Asn 
    130                 135                 140                 

Ile Thr Asn Ser Ile Thr Asn Ser Ser Val Asn Met Arg Glu Glu Ile 
145                 150                 155                 160 

Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg Asp Lys Asn Arg 
                165                 170                 175     

Lys Val Tyr Ser Leu Phe Tyr Lys Leu Asp Val Val Gln Ile Asn Asn 
            180                 185                 190         

Gly Asn Asn Ser Ser Asn Leu Tyr Arg Leu Ile Asn Cys Asn Thr Ser 
        195                 200                 205             

Ala Leu Thr Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile 
    210                 215                 220                 

Arg Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asp Lys 
225                 230                 235                 240 

Glu Phe Asn Gly Thr Gly Leu Cys Lys Asn Val Ser Thr Val Gln Cys 
                245                 250                 255     

Thr His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly 
            260                 265                 270         

Ser Leu Ala Glu Gly Lys Val Met Ile Arg Ser Glu Asn Ile Thr Asn 
        275                 280                 285             

Asn Val Lys Asn Ile Ile Val Gln Leu Asn Glu Thr Val Thr Ile Asn 
    290                 295                 300                 

Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Val Arg Ile Gly Pro 
305                 310                 315                 320 

Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln 
                325                 330                 335     

Ala His Cys Asn Val Ser Gly Ser Gln Trp Asn Arg Ala Leu His Gln 
            340                 345                 350         

Val Val Gly Gln Leu Arg Glu Tyr Trp Asn Thr Thr Ile Ile Phe Lys 
        355                 360                 365             

Asn Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys 
    370                 375                 380                 

Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Asn 
385                 390                 395                 400 

Trp Thr His Asn Asp Thr Ala Ser Met Lys Pro Asn Asp Thr Ile Thr 
                405                 410                 415     

Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Val Gly 
            420                 425                 430         

Gln Ala Ile Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Glu Ser 
        435                 440                 445             

Asn Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Gly Asn Ile Asn 
    450                 455                 460                 

Glu Ser Gln Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp 
465                 470                 475                 480 

Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu Pro Leu Gly 
                485                 490                 495     

Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg 
            500                 505                 510         

Ala Val Val Glu Leu Gly Ala Val Phe Ile Gly Phe Leu Gly Thr Ala 
        515                 520                 525             

Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Val Arg 
    530                 535                 540                 

Lys Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
545                 550                 555                 560 

Ile Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
                565                 570                 575     

Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln 
            580                 585                 590         

Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
        595                 600                 605             

Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Glu Arg Glu Ile 
    610                 615                 620                 

Trp Glu Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
625                 630                 635                 640 

Thr His Ile Ile Tyr Glu Leu Ile Glu Glu Ser Gln Lys Gln Gln Glu 
                645                 650                 655     

Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Asn Leu Trp 
            660                 665                 670         

Asn Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Gly Ser Gly 
        675                 680                 685             

Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala 
    690                 695                 700                 

Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln 
705                 710                 715                 720 

Ala Arg 
        

<210>   67
<211>   730
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   67
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala Glu Thr 
    50                  55                  60                  

Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu Val His 
65                  70                  75                  80  

Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
                85                  90                  95      

Glu Ile Tyr Met Glu Asn Val Thr Glu Glu Phe Asn Met Trp Lys Asn 
            100                 105                 110         

Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser Leu Trp Asp Gln 
        115                 120                 125             

Ser Leu Lys Pro Cys Val Gln Leu Thr Pro Leu Cys Val Thr Leu Asp 
    130                 135                 140                 

Cys Ser Tyr Asn Ile Thr Asn Asn Ile Thr Asn Ser Ile Thr Asn Ser 
145                 150                 155                 160 

Ser Val Asn Met Arg Glu Glu Ile Lys Asn Cys Ser Phe Asn Met Thr 
                165                 170                 175     

Thr Glu Leu Arg Asp Lys Asn Arg Lys Val Tyr Ser Leu Phe Tyr Lys 
            180                 185                 190         

Leu Asp Val Val Gln Ile Asn Asn Gly Asn Asn Ser Ser Asn Leu Tyr 
        195                 200                 205             

Arg Leu Ile Asn Cys Asn Thr Ser Ala Leu Thr Gln Ala Cys Pro Lys 
    210                 215                 220                 

Val Thr Phe Glu Pro Ile Pro Ile Arg Tyr Cys Ala Pro Ala Gly Tyr 
225                 230                 235                 240 

Ala Ile Leu Lys Cys Asn Asp Lys Glu Phe Asn Gly Thr Gly Leu Cys 
                245                 250                 255     

Lys Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val 
            260                 265                 270         

Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Gly Lys Val Met 
        275                 280                 285             

Ile Arg Ser Glu Asn Ile Thr Asn Asn Val Lys Asn Ile Ile Val Gln 
    290                 295                 300                 

Leu Asn Glu Thr Val Thr Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr 
305                 310                 315                 320 

Arg Lys Ser Val Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly 
                325                 330                 335     

Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Gly Ser 
            340                 345                 350         

Gln Trp Asn Arg Ala Leu His Gln Val Val Gly Gln Leu Arg Glu Tyr 
        355                 360                 365             

Trp Asn Thr Thr Ile Ile Phe Lys Asn Ser Ser Gly Gly Asp Leu Glu 
    370                 375                 380                 

Ile Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn 
385                 390                 395                 400 

Thr Ser Gly Leu Phe Asn Ser Asn Trp Thr His Asn Asp Thr Ala Ser 
                405                 410                 415     

Met Lys Pro Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile 
            420                 425                 430         

Ile Asn Met Trp Gln Arg Val Gly Gln Ala Ile Tyr Ala Pro Pro Ile 
        435                 440                 445             

Gln Gly Val Ile Arg Cys Glu Ser Asn Ile Thr Gly Leu Ile Leu Thr 
    450                 455                 460                 

Arg Asp Gly Gly Gly Asn Ile Asn Glu Ser Gln Ile Phe Arg Pro Gly 
465                 470                 475                 480 

Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys 
                485                 490                 495     

Val Val Arg Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg 
            500                 505                 510         

Arg Val Val Glu Arg Glu Lys Arg Ala Val Val Glu Leu Gly Ala Val 
        515                 520                 525             

Phe Ile Gly Phe Leu Gly Thr Ala Gly Ser Thr Met Gly Ala Ala Ser 
    530                 535                 540                 

Ile Thr Leu Thr Val Gln Val Arg Lys Leu Leu Ser Gly Ile Val Gln 
545                 550                 555                 560 

Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu 
                565                 570                 575     

Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala 
            580                 585                 590         

Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys 
        595                 600                 605             

Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp 
    610                 615                 620                 

Ser Asn Lys Ser Glu Arg Glu Ile Trp Glu Asn Met Thr Trp Leu Gln 
625                 630                 635                 640 

Trp Asp Lys Glu Ile Ser Asn Tyr Thr His Ile Ile Tyr Glu Leu Ile 
                645                 650                 655     

Glu Glu Ser Gln Lys Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu 
            660                 665                 670         

Leu Asp Lys Trp Ala Asn Leu Trp Asn Trp Phe Asp Ile Ser Asn Trp 
        675                 680                 685             

Leu Trp Tyr Ile Lys Gly Ser Gly Ser Gly Ile Val Gln Gln Gln Asn 
    690                 695                 700                 

Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr 
705                 710                 715                 720 

Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
                725                 730 

<210>   68
<211>   753
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1Nipah fusion protein

<400>   68
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val 
        35                  40                  45              

Pro Val Trp Lys Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala 
    50                  55                  60                  

Lys Ala Tyr Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys 
65                  70                  75                  80  

Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Tyr Met Glu Asn Val Thr 
                85                  90                  95      

Glu Glu Phe Asn Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr 
            100                 105                 110         

Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Gln Leu 
        115                 120                 125             

Thr Pro Leu Cys Val Thr Leu Asp Cys Ser Tyr Asn Ile Thr Asn Asn 
    130                 135                 140                 

Ile Thr Asn Ser Ile Thr Asn Ser Ser Val Asn Met Arg Glu Glu Ile 
145                 150                 155                 160 

Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg Asp Lys Asn Arg 
                165                 170                 175     

Lys Val Tyr Ser Leu Phe Tyr Lys Leu Asp Val Val Gln Ile Asn Asn 
            180                 185                 190         

Gly Asn Asn Ser Ser Asn Leu Tyr Arg Leu Ile Asn Cys Asn Thr Ser 
        195                 200                 205             

Ala Leu Thr Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile 
    210                 215                 220                 

Arg Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asp Lys 
225                 230                 235                 240 

Glu Phe Asn Gly Thr Gly Leu Cys Lys Asn Val Ser Thr Val Gln Cys 
                245                 250                 255     

Thr His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly 
            260                 265                 270         

Ser Leu Ala Glu Gly Lys Val Met Ile Arg Ser Glu Asn Ile Thr Asn 
        275                 280                 285             

Asn Val Lys Asn Ile Ile Val Gln Leu Asn Glu Thr Val Thr Ile Asn 
    290                 295                 300                 

Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Val Arg Ile Gly Pro 
305                 310                 315                 320 

Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln 
                325                 330                 335     

Ala His Cys Asn Val Ser Gly Ser Gln Trp Asn Arg Ala Leu His Gln 
            340                 345                 350         

Val Val Gly Gln Leu Arg Glu Tyr Trp Asn Thr Thr Ile Ile Phe Lys 
        355                 360                 365             

Asn Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys 
    370                 375                 380                 

Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Asn 
385                 390                 395                 400 

Trp Thr His Asn Asp Thr Ala Ser Met Lys Pro Asn Asp Thr Ile Thr 
                405                 410                 415     

Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Val Gly 
            420                 425                 430         

Gln Ala Ile Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Glu Ser 
        435                 440                 445             

Asn Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Gly Asn Ile Asn 
    450                 455                 460                 

Glu Ser Gln Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp 
465                 470                 475                 480 

Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu Pro Leu Gly 
                485                 490                 495     

Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg 
            500                 505                 510         

Ala Val Val Glu Leu Gly Ala Val Phe Ile Gly Phe Leu Gly Thr Ala 
        515                 520                 525             

Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Val Arg 
    530                 535                 540                 

Lys Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
545                 550                 555                 560 

Ile Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
                565                 570                 575     

Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln 
            580                 585                 590         

Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
        595                 600                 605             

Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Glu Arg Glu Ile 
    610                 615                 620                 

Trp Glu Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
625                 630                 635                 640 

Thr His Ile Ile Tyr Glu Leu Ile Glu Glu Ser Gln Lys Gln Gln Glu 
                645                 650                 655     

Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Asn Leu Trp 
            660                 665                 670         

Asn Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ser Arg Ile 
        675                 680                 685             

Glu Gly Arg Gly Ser Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly 
    690                 695                 700                 

Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe 
705                 710                 715                 720 

Leu Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val Val 
                725                 730                 735     

Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala Leu 
            740                 745                 750         

Gln 
    

<210>   69
<211>   761
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1Nipah fusion protein

<400>   69
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala Glu Thr 
    50                  55                  60                  

Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu Val His 
65                  70                  75                  80  

Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
                85                  90                  95      

Glu Ile Tyr Met Glu Asn Val Thr Glu Glu Phe Asn Met Trp Lys Asn 
            100                 105                 110         

Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser Leu Trp Asp Gln 
        115                 120                 125             

Ser Leu Lys Pro Cys Val Gln Leu Thr Pro Leu Cys Val Thr Leu Asp 
    130                 135                 140                 

Cys Ser Tyr Asn Ile Thr Asn Asn Ile Thr Asn Ser Ile Thr Asn Ser 
145                 150                 155                 160 

Ser Val Asn Met Arg Glu Glu Ile Lys Asn Cys Ser Phe Asn Met Thr 
                165                 170                 175     

Thr Glu Leu Arg Asp Lys Asn Arg Lys Val Tyr Ser Leu Phe Tyr Lys 
            180                 185                 190         

Leu Asp Val Val Gln Ile Asn Asn Gly Asn Asn Ser Ser Asn Leu Tyr 
        195                 200                 205             

Arg Leu Ile Asn Cys Asn Thr Ser Ala Leu Thr Gln Ala Cys Pro Lys 
    210                 215                 220                 

Val Thr Phe Glu Pro Ile Pro Ile Arg Tyr Cys Ala Pro Ala Gly Tyr 
225                 230                 235                 240 

Ala Ile Leu Lys Cys Asn Asp Lys Glu Phe Asn Gly Thr Gly Leu Cys 
                245                 250                 255     

Lys Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val 
            260                 265                 270         

Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Gly Lys Val Met 
        275                 280                 285             

Ile Arg Ser Glu Asn Ile Thr Asn Asn Val Lys Asn Ile Ile Val Gln 
    290                 295                 300                 

Leu Asn Glu Thr Val Thr Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr 
305                 310                 315                 320 

Arg Lys Ser Val Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly 
                325                 330                 335     

Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Gly Ser 
            340                 345                 350         

Gln Trp Asn Arg Ala Leu His Gln Val Val Gly Gln Leu Arg Glu Tyr 
        355                 360                 365             

Trp Asn Thr Thr Ile Ile Phe Lys Asn Ser Ser Gly Gly Asp Leu Glu 
    370                 375                 380                 

Ile Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn 
385                 390                 395                 400 

Thr Ser Gly Leu Phe Asn Ser Asn Trp Thr His Asn Asp Thr Ala Ser 
                405                 410                 415     

Met Lys Pro Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile 
            420                 425                 430         

Ile Asn Met Trp Gln Arg Val Gly Gln Ala Ile Tyr Ala Pro Pro Ile 
        435                 440                 445             

Gln Gly Val Ile Arg Cys Glu Ser Asn Ile Thr Gly Leu Ile Leu Thr 
    450                 455                 460                 

Arg Asp Gly Gly Gly Asn Ile Asn Glu Ser Gln Ile Phe Arg Pro Gly 
465                 470                 475                 480 

Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys 
                485                 490                 495     

Val Val Arg Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg 
            500                 505                 510         

Arg Val Val Glu Arg Glu Lys Arg Ala Val Val Glu Leu Gly Ala Val 
        515                 520                 525             

Phe Ile Gly Phe Leu Gly Thr Ala Gly Ser Thr Met Gly Ala Ala Ser 
    530                 535                 540                 

Ile Thr Leu Thr Val Gln Val Arg Lys Leu Leu Ser Gly Ile Val Gln 
545                 550                 555                 560 

Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu 
                565                 570                 575     

Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala 
            580                 585                 590         

Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys 
        595                 600                 605             

Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp 
    610                 615                 620                 

Ser Asn Lys Ser Glu Arg Glu Ile Trp Glu Asn Met Thr Trp Leu Gln 
625                 630                 635                 640 

Trp Asp Lys Glu Ile Ser Asn Tyr Thr His Ile Ile Tyr Glu Leu Ile 
                645                 650                 655     

Glu Glu Ser Gln Lys Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu 
            660                 665                 670         

Leu Asp Lys Trp Ala Asn Leu Trp Asn Trp Phe Asp Ile Ser Asn Trp 
        675                 680                 685             

Leu Trp Tyr Ile Lys Ser Arg Ile Glu Gly Arg Gly Ser Gly Gly Tyr 
    690                 695                 700                 

Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly 
705                 710                 715                 720 

Glu Trp Val Leu Leu Ser Thr Phe Leu Asn Lys Leu Lys Ser Ser Ile 
                725                 730                 735     

Glu Ser Thr Asn Glu Ala Val Val Lys Leu Gln Glu Thr Ala Glu Lys 
            740                 745                 750         

Thr Val Tyr Val Leu Thr Ala Leu Gln 
        755                 760     

<210>   70
<211>   720
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1Nipah fusion protein

<400>   70
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val 
        35                  40                  45              

Pro Val Trp Lys Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala 
    50                  55                  60                  

Lys Ala Tyr Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys 
65                  70                  75                  80  

Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Tyr Met Glu Asn Val Thr 
                85                  90                  95      

Glu Glu Phe Asn Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr 
            100                 105                 110         

Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Gln Leu 
        115                 120                 125             

Thr Pro Leu Cys Val Thr Leu Asp Cys Ser Tyr Asn Ile Thr Asn Asn 
    130                 135                 140                 

Ile Thr Asn Ser Ile Thr Asn Ser Ser Val Asn Met Arg Glu Glu Ile 
145                 150                 155                 160 

Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg Asp Lys Asn Arg 
                165                 170                 175     

Lys Val Tyr Ser Leu Phe Tyr Lys Leu Asp Val Val Gln Ile Asn Asn 
            180                 185                 190         

Gly Asn Asn Ser Ser Asn Leu Tyr Arg Leu Ile Asn Cys Asn Thr Ser 
        195                 200                 205             

Ala Leu Thr Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile 
    210                 215                 220                 

Arg Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asp Lys 
225                 230                 235                 240 

Glu Phe Asn Gly Thr Gly Leu Cys Lys Asn Val Ser Thr Val Gln Cys 
                245                 250                 255     

Thr His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly 
            260                 265                 270         

Ser Leu Ala Glu Gly Lys Val Met Ile Arg Ser Glu Asn Ile Thr Asn 
        275                 280                 285             

Asn Val Lys Asn Ile Ile Val Gln Leu Asn Glu Thr Val Thr Ile Asn 
    290                 295                 300                 

Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Val Arg Ile Gly Pro 
305                 310                 315                 320 

Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln 
                325                 330                 335     

Ala His Cys Asn Val Ser Gly Ser Gln Trp Asn Arg Ala Leu His Gln 
            340                 345                 350         

Val Val Gly Gln Leu Arg Glu Tyr Trp Asn Thr Thr Ile Ile Phe Lys 
        355                 360                 365             

Asn Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys 
    370                 375                 380                 

Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Asn 
385                 390                 395                 400 

Trp Thr His Asn Asp Thr Ala Ser Met Lys Pro Asn Asp Thr Ile Thr 
                405                 410                 415     

Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Val Gly 
            420                 425                 430         

Gln Ala Ile Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Glu Ser 
        435                 440                 445             

Asn Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Gly Asn Ile Asn 
    450                 455                 460                 

Glu Ser Gln Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp 
465                 470                 475                 480 

Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu Pro Leu Gly 
                485                 490                 495     

Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg 
            500                 505                 510         

Ala Val Val Glu Leu Gly Ala Val Phe Ile Gly Phe Leu Gly Thr Ala 
        515                 520                 525             

Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Val Arg 
    530                 535                 540                 

Lys Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
545                 550                 555                 560 

Ile Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
                565                 570                 575     

Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln 
            580                 585                 590         

Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
        595                 600                 605             

Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Glu Arg Glu Ile 
    610                 615                 620                 

Trp Glu Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
625                 630                 635                 640 

Thr His Ile Ile Tyr Glu Leu Ile Glu Glu Ser Gln Lys Gln Gln Glu 
                645                 650                 655     

Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Asn Leu Trp 
            660                 665                 670         

Asn Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ser Asn Ile 
        675                 680                 685             

Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val Val Lys 
    690                 695                 700                 

Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala Leu Gln 
705                 710                 715                 720 

<210>   71
<211>   728
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 (1992 Uganda, serotype 037.8, clade A) envelope glycoprotein ectodomain (gp140)-HR1Nipah fusion protein

<400>   71
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala Glu Thr 
    50                  55                  60                  

Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu Val His 
65                  70                  75                  80  

Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
                85                  90                  95      

Glu Ile Tyr Met Glu Asn Val Thr Glu Glu Phe Asn Met Trp Lys Asn 
            100                 105                 110         

Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser Leu Trp Asp Gln 
        115                 120                 125             

Ser Leu Lys Pro Cys Val Gln Leu Thr Pro Leu Cys Val Thr Leu Asp 
    130                 135                 140                 

Cys Ser Tyr Asn Ile Thr Asn Asn Ile Thr Asn Ser Ile Thr Asn Ser 
145                 150                 155                 160 

Ser Val Asn Met Arg Glu Glu Ile Lys Asn Cys Ser Phe Asn Met Thr 
                165                 170                 175     

Thr Glu Leu Arg Asp Lys Asn Arg Lys Val Tyr Ser Leu Phe Tyr Lys 
            180                 185                 190         

Leu Asp Val Val Gln Ile Asn Asn Gly Asn Asn Ser Ser Asn Leu Tyr 
        195                 200                 205             

Arg Leu Ile Asn Cys Asn Thr Ser Ala Leu Thr Gln Ala Cys Pro Lys 
    210                 215                 220                 

Val Thr Phe Glu Pro Ile Pro Ile Arg Tyr Cys Ala Pro Ala Gly Tyr 
225                 230                 235                 240 

Ala Ile Leu Lys Cys Asn Asp Lys Glu Phe Asn Gly Thr Gly Leu Cys 
                245                 250                 255     

Lys Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val 
            260                 265                 270         

Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Gly Lys Val Met 
        275                 280                 285             

Ile Arg Ser Glu Asn Ile Thr Asn Asn Val Lys Asn Ile Ile Val Gln 
    290                 295                 300                 

Leu Asn Glu Thr Val Thr Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr 
305                 310                 315                 320 

Arg Lys Ser Val Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly 
                325                 330                 335     

Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Gly Ser 
            340                 345                 350         

Gln Trp Asn Arg Ala Leu His Gln Val Val Gly Gln Leu Arg Glu Tyr 
        355                 360                 365             

Trp Asn Thr Thr Ile Ile Phe Lys Asn Ser Ser Gly Gly Asp Leu Glu 
    370                 375                 380                 

Ile Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn 
385                 390                 395                 400 

Thr Ser Gly Leu Phe Asn Ser Asn Trp Thr His Asn Asp Thr Ala Ser 
                405                 410                 415     

Met Lys Pro Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile 
            420                 425                 430         

Ile Asn Met Trp Gln Arg Val Gly Gln Ala Ile Tyr Ala Pro Pro Ile 
        435                 440                 445             

Gln Gly Val Ile Arg Cys Glu Ser Asn Ile Thr Gly Leu Ile Leu Thr 
    450                 455                 460                 

Arg Asp Gly Gly Gly Asn Ile Asn Glu Ser Gln Ile Phe Arg Pro Gly 
465                 470                 475                 480 

Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys 
                485                 490                 495     

Val Val Arg Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg 
            500                 505                 510         

Arg Val Val Glu Arg Glu Lys Arg Ala Val Val Glu Leu Gly Ala Val 
        515                 520                 525             

Phe Ile Gly Phe Leu Gly Thr Ala Gly Ser Thr Met Gly Ala Ala Ser 
    530                 535                 540                 

Ile Thr Leu Thr Val Gln Val Arg Lys Leu Leu Ser Gly Ile Val Gln 
545                 550                 555                 560 

Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu 
                565                 570                 575     

Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala 
            580                 585                 590         

Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys 
        595                 600                 605             

Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp 
    610                 615                 620                 

Ser Asn Lys Ser Glu Arg Glu Ile Trp Glu Asn Met Thr Trp Leu Gln 
625                 630                 635                 640 

Trp Asp Lys Glu Ile Ser Asn Tyr Thr His Ile Ile Tyr Glu Leu Ile 
                645                 650                 655     

Glu Glu Ser Gln Lys Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu 
            660                 665                 670         

Leu Asp Lys Trp Ala Asn Leu Trp Asn Trp Phe Asp Ile Ser Asn Trp 
        675                 680                 685             

Leu Trp Tyr Ile Lys Ser Asn Ile Asn Lys Leu Lys Ser Ser Ile Glu 
    690                 695                 700                 

Ser Thr Asn Glu Ala Val Val Lys Leu Gln Glu Thr Ala Glu Lys Thr 
705                 710                 715                 720 

Val Tyr Val Leu Thr Ala Leu Gln 
                725             

<210>   72
<211>   741
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 clade C 1997 envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   72
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Gly Asn Met Trp Val Thr 
        35                  40                  45              

Val Tyr Tyr Gly Val Pro Val Trp Thr Asp Ala Lys Thr Thr Leu Phe 
    50                  55                  60                  

Cys Ala Ser Asp Thr Lys Ala Tyr Asp Arg Glu Val His Asn Val Trp 
65                  70                  75                  80  

Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Val 
                85                  90                  95      

Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asp Met Val 
            100                 105                 110         

Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys 
        115                 120                 125             

Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu His Cys Thr Asn 
    130                 135                 140                 

Ala Thr Phe Lys Asn Asn Val Thr Asn Asp Met Asn Lys Glu Ile Arg 
145                 150                 155                 160 

Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg Asp Lys Lys Gln Gln 
                165                 170                 175     

Gly Tyr Ala Leu Phe Tyr Arg Pro Asp Ile Val Leu Leu Lys Glu Asn 
            180                 185                 190         

Arg Asn Asn Ser Asn Asn Ser Glu Tyr Ile Leu Ile Asn Cys Asn Ala 
        195                 200                 205             

Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Asn Phe Asp Pro Ile Pro 
    210                 215                 220                 

Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
225                 230                 235                 240 

Lys Thr Phe Ser Gly Lys Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
                245                 250                 255     

Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
            260                 265                 270         

Gly Ser Leu Ala Glu Lys Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
        275                 280                 285             

Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Lys Ser Val Glu Ile 
    290                 295                 300                 

Val Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Met Arg Ile Gly 
305                 310                 315                 320 

Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
                325                 330                 335     

Gln Ala Tyr Cys Asn Ile Ser Gly Ser Lys Trp Asn Glu Thr Leu Lys 
            340                 345                 350         

Arg Val Lys Glu Lys Leu Gln Glu Asn Tyr Asn Asn Asn Lys Thr Ile 
        355                 360                 365             

Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser 
    370                 375                 380                 

Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Thr Arg Leu Phe 
385                 390                 395                 400 

Asn Asn Asn Ala Thr Glu Asp Glu Thr Ile Thr Leu Pro Cys Arg Ile 
                405                 410                 415     

Lys Gln Ile Ile Asn Met Trp Gln Gly Val Gly Arg Ala Met Tyr Ala 
            420                 425                 430         

Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu 
        435                 440                 445             

Leu Leu Val Arg Asp Gly Gly Glu Asp Asn Lys Thr Glu Glu Ile Phe 
    450                 455                 460                 

Arg Pro Gly Gly Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr 
465                 470                 475                 480 

Lys Tyr Lys Val Ile Glu Leu Lys Pro Leu Gly Ile Ala Pro Thr Gly 
                485                 490                 495     

Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg Ala Val Gly Ile Gly 
            500                 505                 510         

Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala 
        515                 520                 525             

Ala Ser Leu Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Ser Ile 
    530                 535                 540                 

Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His 
545                 550                 555                 560 

Met Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val 
                565                 570                 575     

Leu Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp 
            580                 585                 590         

Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser 
        595                 600                 605             

Ser Trp Ser Asn Lys Ser Gln Thr Asp Ile Trp Asn Asn Met Thr Trp 
    610                 615                 620                 

Met Glu Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asp Thr Ile Tyr Arg 
625                 630                 635                 640 

Leu Leu Glu Asp Ser Gln Thr Gln Gln Glu Lys Asn Glu Lys Asp Leu 
                645                 650                 655     

Leu Ala Leu Asp Ser Trp Lys Asn Leu Trp Ser Trp Phe Asp Ile Ser 
            660                 665                 670         

Asn Trp Leu Trp Tyr Ile Lys Ser Gly Tyr Ile Pro Glu Ala Pro Arg 
        675                 680                 685             

Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
    690                 695                 700                 

Thr Phe Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala 
705                 710                 715                 720 

Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys 
                725                 730                 735     

Gln Leu Gln Ala Arg 
            740     

<210>   73
<211>   749
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 clade C 1997 envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   73
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Gly Asn Met Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp 
    50                  55                  60                  

Thr Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Thr Lys Ala Tyr 
65                  70                  75                  80  

Asp Arg Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr 
                85                  90                  95      

Asp Pro Asn Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe 
            100                 105                 110         

Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile 
        115                 120                 125             

Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu 
    130                 135                 140                 

Cys Val Thr Leu His Cys Thr Asn Ala Thr Phe Lys Asn Asn Val Thr 
145                 150                 155                 160 

Asn Asp Met Asn Lys Glu Ile Arg Asn Cys Ser Phe Asn Thr Thr Thr 
                165                 170                 175     

Glu Ile Arg Asp Lys Lys Gln Gln Gly Tyr Ala Leu Phe Tyr Arg Pro 
            180                 185                 190         

Asp Ile Val Leu Leu Lys Glu Asn Arg Asn Asn Ser Asn Asn Ser Glu 
        195                 200                 205             

Tyr Ile Leu Ile Asn Cys Asn Ala Ser Thr Ile Thr Gln Ala Cys Pro 
    210                 215                 220                 

Lys Val Asn Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
225                 230                 235                 240 

Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys Gly Pro 
                245                 250                 255     

Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
            260                 265                 270         

Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Lys Glu Ile 
        275                 280                 285             

Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val 
    290                 295                 300                 

His Leu Asn Lys Ser Val Glu Ile Val Cys Thr Arg Pro Asn Asn Asn 
305                 310                 315                 320 

Thr Arg Lys Ser Met Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr 
                325                 330                 335     

Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala Tyr Cys Asn Ile Ser Gly 
            340                 345                 350         

Ser Lys Trp Asn Glu Thr Leu Lys Arg Val Lys Glu Lys Leu Gln Glu 
        355                 360                 365             

Asn Tyr Asn Asn Asn Lys Thr Ile Lys Phe Ala Pro Ser Ser Gly Gly 
    370                 375                 380                 

Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
385                 390                 395                 400 

Tyr Cys Asn Thr Thr Arg Leu Phe Asn Asn Asn Ala Thr Glu Asp Glu 
                405                 410                 415     

Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
            420                 425                 430         

Gly Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
        435                 440                 445             

Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Val Arg Asp Gly Gly Glu 
    450                 455                 460                 

Asp Asn Lys Thr Glu Glu Ile Phe Arg Pro Gly Gly Gly Asn Met Lys 
465                 470                 475                 480 

Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Ile Glu Leu Lys 
                485                 490                 495     

Pro Leu Gly Ile Ala Pro Thr Gly Ala Lys Arg Arg Val Val Glu Arg 
            500                 505                 510         

Glu Lys Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly 
        515                 520                 525             

Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Leu Thr Leu Thr Val Gln 
    530                 535                 540                 

Ala Arg Gln Leu Leu Ser Ser Ile Val Gln Gln Gln Ser Asn Leu Leu 
545                 550                 555                 560 

Arg Ala Ile Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     

Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys 
            580                 585                 590         

Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
        595                 600                 605             

Thr Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Thr 
    610                 615                 620                 

Asp Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 

Asn Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp Ser Gln Thr Gln 
                645                 650                 655     

Gln Glu Lys Asn Glu Lys Asp Leu Leu Ala Leu Asp Ser Trp Lys Asn 
            660                 665                 670         

Leu Trp Ser Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ser 
        675                 680                 685             

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
    690                 695                 700                 

Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Ser Gly Ile Val Gln 
705                 710                 715                 720 

Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu 
                725                 730                 735     

Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
            740                 745                 

<210>   74
<211>   716
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 clade C 1997 envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   74
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Gly Asn Met Trp Val Thr 
        35                  40                  45              

Val Tyr Tyr Gly Val Pro Val Trp Thr Asp Ala Lys Thr Thr Leu Phe 
    50                  55                  60                  

Cys Ala Ser Asp Thr Lys Ala Tyr Asp Arg Glu Val His Asn Val Trp 
65                  70                  75                  80  

Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Val 
                85                  90                  95      

Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asp Met Val 
            100                 105                 110         

Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys 
        115                 120                 125             

Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu His Cys Thr Asn 
    130                 135                 140                 

Ala Thr Phe Lys Asn Asn Val Thr Asn Asp Met Asn Lys Glu Ile Arg 
145                 150                 155                 160 

Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg Asp Lys Lys Gln Gln 
                165                 170                 175     

Gly Tyr Ala Leu Phe Tyr Arg Pro Asp Ile Val Leu Leu Lys Glu Asn 
            180                 185                 190         

Arg Asn Asn Ser Asn Asn Ser Glu Tyr Ile Leu Ile Asn Cys Asn Ala 
        195                 200                 205             

Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Asn Phe Asp Pro Ile Pro 
    210                 215                 220                 

Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
225                 230                 235                 240 

Lys Thr Phe Ser Gly Lys Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
                245                 250                 255     

Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
            260                 265                 270         

Gly Ser Leu Ala Glu Lys Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
        275                 280                 285             

Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Lys Ser Val Glu Ile 
    290                 295                 300                 

Val Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Met Arg Ile Gly 
305                 310                 315                 320 

Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
                325                 330                 335     

Gln Ala Tyr Cys Asn Ile Ser Gly Ser Lys Trp Asn Glu Thr Leu Lys 
            340                 345                 350         

Arg Val Lys Glu Lys Leu Gln Glu Asn Tyr Asn Asn Asn Lys Thr Ile 
        355                 360                 365             

Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser 
    370                 375                 380                 

Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Thr Arg Leu Phe 
385                 390                 395                 400 

Asn Asn Asn Ala Thr Glu Asp Glu Thr Ile Thr Leu Pro Cys Arg Ile 
                405                 410                 415     

Lys Gln Ile Ile Asn Met Trp Gln Gly Val Gly Arg Ala Met Tyr Ala 
            420                 425                 430         

Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu 
        435                 440                 445             

Leu Leu Val Arg Asp Gly Gly Glu Asp Asn Lys Thr Glu Glu Ile Phe 
    450                 455                 460                 

Arg Pro Gly Gly Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr 
465                 470                 475                 480 

Lys Tyr Lys Val Ile Glu Leu Lys Pro Leu Gly Ile Ala Pro Thr Gly 
                485                 490                 495     

Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg Ala Val Gly Ile Gly 
            500                 505                 510         

Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala 
        515                 520                 525             

Ala Ser Leu Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Ser Ile 
    530                 535                 540                 

Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His 
545                 550                 555                 560 

Met Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val 
                565                 570                 575     

Leu Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp 
            580                 585                 590         

Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser 
        595                 600                 605             

Ser Trp Ser Asn Lys Ser Gln Thr Asp Ile Trp Asn Asn Met Thr Trp 
    610                 615                 620                 

Met Glu Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asp Thr Ile Tyr Arg 
625                 630                 635                 640 

Leu Leu Glu Asp Ser Gln Thr Gln Gln Glu Lys Asn Glu Lys Asp Leu 
                645                 650                 655     

Leu Ala Leu Asp Ser Trp Lys Asn Leu Trp Ser Trp Phe Asp Ile Ser 
            660                 665                 670         

Asn Trp Leu Trp Tyr Ile Lys Ser Gly Ile Ser Gly Ile Val Gln Gln 
        675                 680                 685             

Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
    690                 695                 700                 

Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
705                 710                 715     

<210>   75
<211>   724
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 clade C 1997 envelope glycoprotein ectodomain (gp140)-HR1HXB2 fusion protein

<400>   75
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Gly Asn Met Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp 
    50                  55                  60                  

Thr Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Thr Lys Ala Tyr 
65                  70                  75                  80  

Asp Arg Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr 
                85                  90                  95      

Asp Pro Asn Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe 
            100                 105                 110         

Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile 
        115                 120                 125             

Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu 
    130                 135                 140                 

Cys Val Thr Leu His Cys Thr Asn Ala Thr Phe Lys Asn Asn Val Thr 
145                 150                 155                 160 

Asn Asp Met Asn Lys Glu Ile Arg Asn Cys Ser Phe Asn Thr Thr Thr 
                165                 170                 175     

Glu Ile Arg Asp Lys Lys Gln Gln Gly Tyr Ala Leu Phe Tyr Arg Pro 
            180                 185                 190         

Asp Ile Val Leu Leu Lys Glu Asn Arg Asn Asn Ser Asn Asn Ser Glu 
        195                 200                 205             

Tyr Ile Leu Ile Asn Cys Asn Ala Ser Thr Ile Thr Gln Ala Cys Pro 
    210                 215                 220                 

Lys Val Asn Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
225                 230                 235                 240 

Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys Gly Pro 
                245                 250                 255     

Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
            260                 265                 270         

Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Lys Glu Ile 
        275                 280                 285             

Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val 
    290                 295                 300                 

His Leu Asn Lys Ser Val Glu Ile Val Cys Thr Arg Pro Asn Asn Asn 
305                 310                 315                 320 

Thr Arg Lys Ser Met Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr 
                325                 330                 335     

Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala Tyr Cys Asn Ile Ser Gly 
            340                 345                 350         

Ser Lys Trp Asn Glu Thr Leu Lys Arg Val Lys Glu Lys Leu Gln Glu 
        355                 360                 365             

Asn Tyr Asn Asn Asn Lys Thr Ile Lys Phe Ala Pro Ser Ser Gly Gly 
    370                 375                 380                 

Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
385                 390                 395                 400 

Tyr Cys Asn Thr Thr Arg Leu Phe Asn Asn Asn Ala Thr Glu Asp Glu 
                405                 410                 415     

Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
            420                 425                 430         

Gly Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
        435                 440                 445             

Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Val Arg Asp Gly Gly Glu 
    450                 455                 460                 

Asp Asn Lys Thr Glu Glu Ile Phe Arg Pro Gly Gly Gly Asn Met Lys 
465                 470                 475                 480 

Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Ile Glu Leu Lys 
                485                 490                 495     

Pro Leu Gly Ile Ala Pro Thr Gly Ala Lys Arg Arg Val Val Glu Arg 
            500                 505                 510         

Glu Lys Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly 
        515                 520                 525             

Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Leu Thr Leu Thr Val Gln 
    530                 535                 540                 

Ala Arg Gln Leu Leu Ser Ser Ile Val Gln Gln Gln Ser Asn Leu Leu 
545                 550                 555                 560 

Arg Ala Ile Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     

Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys 
            580                 585                 590         

Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
        595                 600                 605             

Thr Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Thr 
    610                 615                 620                 

Asp Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 

Asn Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp Ser Gln Thr Gln 
                645                 650                 655     

Gln Glu Lys Asn Glu Lys Asp Leu Leu Ala Leu Asp Ser Trp Lys Asn 
            660                 665                 670         

Leu Trp Ser Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ser 
        675                 680                 685             

Gly Ile Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile 
    690                 695                 700                 

Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
705                 710                 715                 720 

Leu Gln Ala Arg 
                

<210>   76
<211>   739
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 clade C 1997 envelope glycoprotein ectodomain (gp140)-HR1Nipah fusion protein

<400>   76
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Gly Asn Met Trp Val Thr 
        35                  40                  45              

Val Tyr Tyr Gly Val Pro Val Trp Thr Asp Ala Lys Thr Thr Leu Phe 
    50                  55                  60                  

Cys Ala Ser Asp Thr Lys Ala Tyr Asp Arg Glu Val His Asn Val Trp 
65                  70                  75                  80  

Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Val 
                85                  90                  95      

Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asp Met Val 
            100                 105                 110         

Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys 
        115                 120                 125             

Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu His Cys Thr Asn 
    130                 135                 140                 

Ala Thr Phe Lys Asn Asn Val Thr Asn Asp Met Asn Lys Glu Ile Arg 
145                 150                 155                 160 

Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg Asp Lys Lys Gln Gln 
                165                 170                 175     

Gly Tyr Ala Leu Phe Tyr Arg Pro Asp Ile Val Leu Leu Lys Glu Asn 
            180                 185                 190         

Arg Asn Asn Ser Asn Asn Ser Glu Tyr Ile Leu Ile Asn Cys Asn Ala 
        195                 200                 205             

Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Asn Phe Asp Pro Ile Pro 
    210                 215                 220                 

Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
225                 230                 235                 240 

Lys Thr Phe Ser Gly Lys Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
                245                 250                 255     

Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
            260                 265                 270         

Gly Ser Leu Ala Glu Lys Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
        275                 280                 285             

Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Lys Ser Val Glu Ile 
    290                 295                 300                 

Val Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Met Arg Ile Gly 
305                 310                 315                 320 

Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
                325                 330                 335     

Gln Ala Tyr Cys Asn Ile Ser Gly Ser Lys Trp Asn Glu Thr Leu Lys 
            340                 345                 350         

Arg Val Lys Glu Lys Leu Gln Glu Asn Tyr Asn Asn Asn Lys Thr Ile 
        355                 360                 365             

Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser 
    370                 375                 380                 

Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Thr Arg Leu Phe 
385                 390                 395                 400 

Asn Asn Asn Ala Thr Glu Asp Glu Thr Ile Thr Leu Pro Cys Arg Ile 
                405                 410                 415     

Lys Gln Ile Ile Asn Met Trp Gln Gly Val Gly Arg Ala Met Tyr Ala 
            420                 425                 430         

Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu 
        435                 440                 445             

Leu Leu Val Arg Asp Gly Gly Glu Asp Asn Lys Thr Glu Glu Ile Phe 
    450                 455                 460                 

Arg Pro Gly Gly Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr 
465                 470                 475                 480 

Lys Tyr Lys Val Ile Glu Leu Lys Pro Leu Gly Ile Ala Pro Thr Gly 
                485                 490                 495     

Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg Ala Val Gly Ile Gly 
            500                 505                 510         

Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala 
        515                 520                 525             

Ala Ser Leu Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Ser Ile 
    530                 535                 540                 

Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His 
545                 550                 555                 560 

Met Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val 
                565                 570                 575     

Leu Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp 
            580                 585                 590         

Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser 
        595                 600                 605             

Ser Trp Ser Asn Lys Ser Gln Thr Asp Ile Trp Asn Asn Met Thr Trp 
    610                 615                 620                 

Met Glu Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asp Thr Ile Tyr Arg 
625                 630                 635                 640 

Leu Leu Glu Asp Ser Gln Thr Gln Gln Glu Lys Asn Glu Lys Asp Leu 
                645                 650                 655     

Leu Ala Leu Asp Ser Trp Lys Asn Leu Trp Ser Trp Phe Asp Ile Ser 
            660                 665                 670         

Asn Trp Leu Trp Tyr Ile Lys Ser Gly Tyr Ile Pro Glu Ala Pro Arg 
        675                 680                 685             

Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
    690                 695                 700                 

Thr Phe Leu Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala 
705                 710                 715                 720 

Val Val Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr 
                725                 730                 735     

Ala Leu Gln 
            

<210>   77
<211>   747
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 clade C 1997 envelope glycoprotein ectodomain (gp140)-HR1Nipah fusion protein

<400>   77
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Gly Asn Met Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp 
    50                  55                  60                  

Thr Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Thr Lys Ala Tyr 
65                  70                  75                  80  

Asp Arg Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr 
                85                  90                  95      

Asp Pro Asn Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe 
            100                 105                 110         

Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile 
        115                 120                 125             

Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu 
    130                 135                 140                 

Cys Val Thr Leu His Cys Thr Asn Ala Thr Phe Lys Asn Asn Val Thr 
145                 150                 155                 160 

Asn Asp Met Asn Lys Glu Ile Arg Asn Cys Ser Phe Asn Thr Thr Thr 
                165                 170                 175     

Glu Ile Arg Asp Lys Lys Gln Gln Gly Tyr Ala Leu Phe Tyr Arg Pro 
            180                 185                 190         

Asp Ile Val Leu Leu Lys Glu Asn Arg Asn Asn Ser Asn Asn Ser Glu 
        195                 200                 205             

Tyr Ile Leu Ile Asn Cys Asn Ala Ser Thr Ile Thr Gln Ala Cys Pro 
    210                 215                 220                 

Lys Val Asn Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
225                 230                 235                 240 

Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys Gly Pro 
                245                 250                 255     

Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
            260                 265                 270         

Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Lys Glu Ile 
        275                 280                 285             

Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val 
    290                 295                 300                 

His Leu Asn Lys Ser Val Glu Ile Val Cys Thr Arg Pro Asn Asn Asn 
305                 310                 315                 320 

Thr Arg Lys Ser Met Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr 
                325                 330                 335     

Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala Tyr Cys Asn Ile Ser Gly 
            340                 345                 350         

Ser Lys Trp Asn Glu Thr Leu Lys Arg Val Lys Glu Lys Leu Gln Glu 
        355                 360                 365             

Asn Tyr Asn Asn Asn Lys Thr Ile Lys Phe Ala Pro Ser Ser Gly Gly 
    370                 375                 380                 

Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
385                 390                 395                 400 

Tyr Cys Asn Thr Thr Arg Leu Phe Asn Asn Asn Ala Thr Glu Asp Glu 
                405                 410                 415     

Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
            420                 425                 430         

Gly Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
        435                 440                 445             

Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Val Arg Asp Gly Gly Glu 
    450                 455                 460                 

Asp Asn Lys Thr Glu Glu Ile Phe Arg Pro Gly Gly Gly Asn Met Lys 
465                 470                 475                 480 

Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Ile Glu Leu Lys 
                485                 490                 495     

Pro Leu Gly Ile Ala Pro Thr Gly Ala Lys Arg Arg Val Val Glu Arg 
            500                 505                 510         

Glu Lys Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly 
        515                 520                 525             

Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Leu Thr Leu Thr Val Gln 
    530                 535                 540                 

Ala Arg Gln Leu Leu Ser Ser Ile Val Gln Gln Gln Ser Asn Leu Leu 
545                 550                 555                 560 

Arg Ala Ile Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     

Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys 
            580                 585                 590         

Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
        595                 600                 605             

Thr Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Thr 
    610                 615                 620                 

Asp Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 

Asn Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp Ser Gln Thr Gln 
                645                 650                 655     

Gln Glu Lys Asn Glu Lys Asp Leu Leu Ala Leu Asp Ser Trp Lys Asn 
            660                 665                 670         

Leu Trp Ser Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ser 
        675                 680                 685             

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
    690                 695                 700                 

Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Asn Lys Leu Lys Ser 
705                 710                 715                 720 

Ser Ile Glu Ser Thr Asn Glu Ala Val Val Lys Leu Gln Glu Thr Ala 
                725                 730                 735     

Glu Lys Thr Val Tyr Val Leu Thr Ala Leu Gln 
            740                 745         

<210>   78
<211>   714
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered HIV-1 clade C 1997 envelope glycoprotein ectodomain (gp140)-HR1Nipah fusion protein

<400>   78
Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      

Ser Tyr Ile Tyr Ala Glu Asp His His His His His His Glu Asn Leu 
            20                  25                  30          

Tyr Phe Gln Gly Ala Glu Asn Leu Trp Val Gly Asn Met Trp Val Thr 
        35                  40                  45              

Val Tyr Tyr Gly Val Pro Val Trp Thr Asp Ala Lys Thr Thr Leu Phe 
    50                  55                  60                  

Cys Ala Ser Asp Thr Lys Ala Tyr Asp Arg Glu Val His Asn Val Trp 
65                  70                  75                  80  

Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Val 
                85                  90                  95      

Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asp Met Val 
            100                 105                 110         

Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys 
        115                 120                 125             

Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu His Cys Thr Asn 
    130                 135                 140                 

Ala Thr Phe Lys Asn Asn Val Thr Asn Asp Met Asn Lys Glu Ile Arg 
145                 150                 155                 160 

Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg Asp Lys Lys Gln Gln 
                165                 170                 175     

Gly Tyr Ala Leu Phe Tyr Arg Pro Asp Ile Val Leu Leu Lys Glu Asn 
            180                 185                 190         

Arg Asn Asn Ser Asn Asn Ser Glu Tyr Ile Leu Ile Asn Cys Asn Ala 
        195                 200                 205             

Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Asn Phe Asp Pro Ile Pro 
    210                 215                 220                 

Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
225                 230                 235                 240 

Lys Thr Phe Ser Gly Lys Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
                245                 250                 255     

Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
            260                 265                 270         

Gly Ser Leu Ala Glu Lys Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
        275                 280                 285             

Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Lys Ser Val Glu Ile 
    290                 295                 300                 

Val Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Met Arg Ile Gly 
305                 310                 315                 320 

Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
                325                 330                 335     

Gln Ala Tyr Cys Asn Ile Ser Gly Ser Lys Trp Asn Glu Thr Leu Lys 
            340                 345                 350         

Arg Val Lys Glu Lys Leu Gln Glu Asn Tyr Asn Asn Asn Lys Thr Ile 
        355                 360                 365             

Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser 
    370                 375                 380                 

Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Thr Arg Leu Phe 
385                 390                 395                 400 

Asn Asn Asn Ala Thr Glu Asp Glu Thr Ile Thr Leu Pro Cys Arg Ile 
                405                 410                 415     

Lys Gln Ile Ile Asn Met Trp Gln Gly Val Gly Arg Ala Met Tyr Ala 
            420                 425                 430         

Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu 
        435                 440                 445             

Leu Leu Val Arg Asp Gly Gly Glu Asp Asn Lys Thr Glu Glu Ile Phe 
    450                 455                 460                 

Arg Pro Gly Gly Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr 
465                 470                 475                 480 

Lys Tyr Lys Val Ile Glu Leu Lys Pro Leu Gly Ile Ala Pro Thr Gly 
                485                 490                 495     

Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg Ala Val Gly Ile Gly 
            500                 505                 510         

Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala 
        515                 520                 525             

Ala Ser Leu Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Ser Ile 
    530                 535                 540                 

Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His 
545                 550                 555                 560 

Met Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val 
                565                 570                 575     

Leu Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp 
            580                 585                 590         

Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser 
        595                 600                 605             

Ser Trp Ser Asn Lys Ser Gln Thr Asp Ile Trp Asn Asn Met Thr Trp 
    610                 615                 620                 

Met Glu Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asp Thr Ile Tyr Arg 
625                 630                 635                 640 

Leu Leu Glu Asp Ser Gln Thr Gln Gln Glu Lys Asn Glu Lys Asp Leu 
                645                 650                 655     

Leu Ala Leu Asp Ser Trp Lys Asn Leu Trp Ser Trp Phe Asp Ile Ser 
            660                 665                 670         

Asn Trp Leu Trp Tyr Ile Lys Ser Asn Ile Asn Lys Leu Lys Ser Ser 
        675                 680                 685             

Ile Glu Ser Thr Asn Glu Ala Val Val Lys Leu Gln Glu Thr Ala Glu 
    690                 695                 700                 

Lys Thr Val Tyr Val Leu Thr Ala Leu Gln 
705                 710                 

<210>   79
<211>   722
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Engineered Rhesus Rotavirus VP7-HR2HXB2 fusion protein

<400>   79
Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      

Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala Glu Asp His 
            20                  25                  30          

His His His His His Glu Asn Leu Tyr Phe Gln Gly Ala Glu Asn Leu 
        35                  40                  45              

Trp Val Gly Asn Met Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp 
    50                  55                  60                  

Thr Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Thr Lys Ala Tyr 
65                  70                  75                  80  

Asp Arg Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr 
                85                  90                  95      

Asp Pro Asn Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe 
            100                 105                 110         

Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile 
        115                 120                 125             

Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu 
    130                 135                 140                 

Cys Val Thr Leu His Cys Thr Asn Ala Thr Phe Lys Asn Asn Val Thr 
145                 150                 155                 160 

Asn Asp Met Asn Lys Glu Ile Arg Asn Cys Ser Phe Asn Thr Thr Thr 
                165                 170                 175     

Glu Ile Arg Asp Lys Lys Gln Gln Gly Tyr Ala Leu Phe Tyr Arg Pro 
            180                 185                 190         

Asp Ile Val Leu Leu Lys Glu Asn Arg Asn Asn Ser Asn Asn Ser Glu 
        195                 200                 205             

Tyr Ile Leu Ile Asn Cys Asn Ala Ser Thr Ile Thr Gln Ala Cys Pro 
    210                 215                 220                 

Lys Val Asn Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
225                 230                 235                 240 

Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys Gly Pro 
                245                 250                 255     

Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
            260                 265                 270         

Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Lys Glu Ile 
        275                 280                 285             

Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val 
    290                 295                 300                 

His Leu Asn Lys Ser Val Glu Ile Val Cys Thr Arg Pro Asn Asn Asn 
305                 310                 315                 320 

Thr Arg Lys Ser Met Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr 
                325                 330                 335     

Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala Tyr Cys Asn Ile Ser Gly 
            340                 345                 350         

Ser Lys Trp Asn Glu Thr Leu Lys Arg Val Lys Glu Lys Leu Gln Glu 
        355                 360                 365             

Asn Tyr Asn Asn Asn Lys Thr Ile Lys Phe Ala Pro Ser Ser Gly Gly 
    370                 375                 380                 

Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
385                 390                 395                 400 

Tyr Cys Asn Thr Thr Arg Leu Phe Asn Asn Asn Ala Thr Glu Asp Glu 
                405                 410                 415     

Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
            420                 425                 430         

Gly Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
        435                 440                 445             

Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Val Arg Asp Gly Gly Glu 
    450                 455                 460                 

Asp Asn Lys Thr Glu Glu Ile Phe Arg Pro Gly Gly Gly Asn Met Lys 
465                 470                 475                 480 

Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Ile Glu Leu Lys 
                485                 490                 495     

Pro Leu Gly Ile Ala Pro Thr Gly Ala Lys Arg Arg Val Val Glu Arg 
            500                 505                 510         

Glu Lys Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly 
        515                 520                 525             

Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Leu Thr Leu Thr Val Gln 
    530                 535                 540                 

Ala Arg Gln Leu Leu Ser Ser Ile Val Gln Gln Gln Ser Asn Leu Leu 
545                 550                 555                 560 

Arg Ala Ile Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     

Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys 
            580                 585                 590         

Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
        595                 600                 605             

Thr Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Thr 
    610                 615                 620                 

Asp Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 

Asn Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp Ser Gln Thr Gln 
                645                 650                 655     

Gln Glu Lys Asn Glu Lys Asp Leu Leu Ala Leu Asp Ser Trp Lys Asn 
            660                 665                 670         

Leu Trp Ser Trp Phe Asp Ile Ser Asn Trp Leu Trp Tyr Ile Lys Ser 
        675                 680                 685             

Asn Ile Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val 
    690                 695                 700                 

Val Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala 
705                 710                 715                 720 

Leu Gln 
        

<210>   80
<211>   282
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   CR6261 ScFv (for bacterial expression)

<400>   80
Met Glu Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly 
1               5                   10                  15      

Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Pro Phe Arg Ser 
            20                  25                  30          

Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Pro Glu Trp 
        35                  40                  45              

Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Thr Lys Tyr Ala Pro Lys 
    50                  55                  60                  

Phe Gln Gly Arg Val Thr Ile Thr Ala Asp Asp Phe Ala Gly Thr Val 
65                  70                  75                  80  

Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr 
                85                  90                  95      

Cys Ala Lys His Met Gly Tyr Gln Val Arg Glu Thr Met Asp Val Trp 
            100                 105                 110         

Gly Lys Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             

Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro 
    130                 135                 140                 

Pro Ser Val Ser Ala Ala Pro Gly Gln Lys Val Thr Ile Ser Cys Ser 
145                 150                 155                 160 

Gly Ser Ser Ser Asn Ile Gly Asn Asp Tyr Val Ser Trp Tyr Gln Gln 
                165                 170                 175     

Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr Asp Asn Asn Lys Arg 
            180                 185                 190         

Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser 
        195                 200                 205             

Ala Thr Leu Gly Ile Thr Gly Leu Gln Thr Gly Asp Glu Ala Asn Tyr 
    210                 215                 220                 

Tyr Cys Ala Thr Trp Asp Arg Arg Pro Thr Ala Tyr Val Val Phe Gly 
225                 230                 235                 240 

Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Gly Gln Pro Lys 
                245                 250                 255     

Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln 
            260                 265                 270         

Arg Gly Ala Ala His His His His His His 
        275                 280         

<210>   81
<211>   301
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   CR6261 scFv (for mammalian expression) 

<400>   81
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      

Ala Val Phe Val Ser Pro Ser Gln Ser Val Leu Thr Gln Pro Pro Ser 
            20                  25                  30          

Val Ser Ala Ala Pro Gly Gln Lys Val Thr Ile Ser Cys Ser Gly Ser 
        35                  40                  45              

Ser Ser Asn Ile Gly Asn Asp Tyr Val Ser Trp Tyr Gln Gln Leu Pro 
    50                  55                  60                  

Gly Thr Ala Pro Lys Leu Leu Ile Tyr Asp Asn Asn Lys Arg Pro Ser 
65                  70                  75                  80  

Gly Ile Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Thr 
                85                  90                  95      

Leu Gly Ile Thr Gly Leu Gln Thr Gly Asp Glu Ala Asn Tyr Tyr Cys 
            100                 105                 110         

Ala Thr Trp Asp Arg Arg Pro Thr Ala Tyr Val Val Phe Gly Gly Gly 
        115                 120                 125             

Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Gly Gln Pro Lys Ala Ala 
    130                 135                 140                 

Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Gly Gly 
145                 150                 155                 160 

Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Glu Val Gln Leu Val Glu 
                165                 170                 175     

Ser Gly Ala Glu Val Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys 
            180                 185                 190         

Lys Ala Ser Gly Gly Pro Phe Arg Ser Tyr Ala Ile Ser Trp Val Arg 
        195                 200                 205             

Gln Ala Pro Gly Gln Gly Pro Glu Trp Met Gly Gly Ile Ile Pro Ile 
    210                 215                 220                 

Phe Gly Thr Thr Lys Tyr Ala Pro Lys Phe Gln Gly Arg Val Thr Ile 
225                 230                 235                 240 

Thr Ala Asp Asp Phe Ala Gly Thr Val Tyr Met Glu Leu Ser Ser Leu 
                245                 250                 255     

Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Lys His Met Gly Tyr 
            260                 265                 270         

Gln Val Arg Glu Thr Met Asp Val Trp Gly Lys Gly Thr Thr Val Thr 
        275                 280                 285             

Val Ser Ser Arg Gly Ala Ala His His His His His His 
    290                 295                 300     

<210>   82
<211>   244
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   CR6261 Lignt Chain (Fab) 

<400>   82
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      

Ala Val Phe Val Ser Pro Ser Gln Ser Val Leu Thr Gln Pro Pro Ser 
            20                  25                  30          

Val Ser Ala Ala Pro Gly Gln Lys Val Thr Ile Ser Cys Ser Gly Ser 
        35                  40                  45              

Ser Ser Asn Ile Gly Asn Asp Tyr Val Ser Trp Tyr Gln Gln Leu Pro 
    50                  55                  60                  

Gly Thr Ala Pro Lys Leu Leu Ile Tyr Asp Asn Asn Lys Arg Pro Ser 
65                  70                  75                  80  

Gly Ile Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Thr 
                85                  90                  95      

Leu Gly Ile Thr Gly Leu Gln Thr Gly Asp Glu Ala Asn Tyr Tyr Cys 
            100                 105                 110         

Ala Thr Trp Asp Arg Arg Pro Thr Ala Tyr Val Val Phe Gly Gly Gly 
        115                 120                 125             

Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Gly Gln Pro Lys Ala Ala 
    130                 135                 140                 

Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn 
145                 150                 155                 160 

Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val 
                165                 170                 175     

Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu 
            180                 185                 190         

Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser 
        195                 200                 205             

Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg Ser Tyr Ser 
    210                 215                 220                 

Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro 
225                 230                 235                 240 

Thr Glu Cys Ser 
                

<210>   83
<211>   249
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   CR6261 Heavy Chain (Fab) 

<400>   83
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      

Ala Val Phe Val Ser Pro Ser Glu Val Gln Leu Val Glu Ser Gly Ala 
            20                  25                  30          

Glu Val Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser 
        35                  40                  45              

Gly Gly Pro Phe Arg Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro 
    50                  55                  60                  

Gly Gln Gly Pro Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr 
65                  70                  75                  80  

Thr Lys Tyr Ala Pro Lys Phe Gln Gly Arg Val Thr Ile Thr Ala Asp 
                85                  90                  95      

Asp Phe Ala Gly Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu 
            100                 105                 110         

Asp Thr Ala Met Tyr Tyr Cys Ala Lys His Met Gly Tyr Gln Val Arg 
        115                 120                 125             

Glu Thr Met Asp Val Trp Gly Lys Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
145                 150                 155                 160 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
                165                 170                 175     

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
            180                 185                 190         

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
        195                 200                 205             

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
    210                 215                 220                 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
225                 230                 235                 240 

Arg Val Glu Pro Lys Ser Cys Asp Lys 
                245                 

