                         SEQUENCE LISTING

<110>  H. Lee Moffitt Cancer Center and Research Institute
 
<120>  SOLUBLE CD33 FOR TREATING MYELODYSPLASTIC SYNDROMES (MDS)

<130>  10110-061WO1

<150>  US 61/843,274
<151>  2013-07-05

<150>  US 61/930,798
<151>  2014-01-23

<150>  US 61/931,366
<151>  2014-01-24

<150>  US . 61/978,009
<151>  2014-04-10

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  242
<212>  PRT
<213>  Homo sapiens

<400>  1

Asp Pro Asn Phe Trp Leu Gln Val Gln Glu Ser Val Thr Val Gln Glu 
1               5                   10                  15      


Gly Leu Cys Val Leu Val Pro Cys Thr Phe Phe His Pro Ile Pro Tyr 
            20                  25                  30          


Tyr Asp Lys Asn Ser Pro Val His Gly Tyr Trp Phe Arg Glu Gly Ala 
        35                  40                  45              


Ile Ile Ser Arg Asp Ser Pro Val Ala Thr Asn Lys Leu Asp Gln Glu 
    50                  55                  60                  


Val Gln Glu Glu Thr Gln Gly Arg Phe Arg Leu Leu Gly Asp Pro Ser 
65                  70                  75                  80  


Arg Asn Asn Cys Ser Leu Ser Ile Val Asp Ala Arg Arg Arg Asp Asn 
                85                  90                  95      


Gly Ser Tyr Phe Phe Arg Met Glu Arg Gly Ser Thr Lys Tyr Ser Tyr 
            100                 105                 110         


Lys Ser Pro Gln Leu Ser Val His Val Thr Asp Leu Thr His Arg Pro 
        115                 120                 125             


Lys Ile Leu Ile Pro Gly Thr Leu Glu Pro Gly His Ser Lys Asn Leu 
    130                 135                 140                 


Thr Cys Ser Val Ser Trp Ala Cys Glu Gln Gly Thr Pro Pro Ile Phe 
145                 150                 155                 160 


Ser Trp Leu Ser Ala Ala Pro Thr Ser Leu Gly Pro Arg Thr Thr His 
                165                 170                 175     


Ser Ser Val Leu Ile Ile Thr Pro Arg Pro Gln Asp His Gly Thr Asn 
            180                 185                 190         


Leu Thr Cys Gln Val Lys Phe Ala Gly Ala Gly Val Thr Thr Glu Arg 
        195                 200                 205             


Thr Ile Gln Leu Asn Val Thr Tyr Val Pro Gln Asn Pro Thr Thr Gly 
    210                 215                 220                 


Ile Phe Pro Gly Asp Gly Ser Gly Lys Gln Glu Thr Arg Ala Gly Val 
225                 230                 235                 240 


Val His 
        


<210>  2
<211>  242
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Trp or Arg

<220>
<221>  MISC_FEATURE
<222>  (52)..(52)
<223>  Xaa is Arg or Gly

<220>
<221>  MISC_FEATURE
<222>  (111)..(111)
<223>  Xaa is Ser or Asn

<400>  2

Asp Pro Asn Phe Xaa Leu Gln Val Gln Glu Ser Val Thr Val Gln Glu 
1               5                   10                  15      


Gly Leu Cys Val Leu Val Pro Cys Thr Phe Phe His Pro Ile Pro Tyr 
            20                  25                  30          


Tyr Asp Lys Asn Ser Pro Val His Gly Tyr Trp Phe Arg Glu Gly Ala 
        35                  40                  45              


Ile Ile Ser Xaa Asp Ser Pro Val Ala Thr Asn Lys Leu Asp Gln Glu 
    50                  55                  60                  


Val Gln Glu Glu Thr Gln Gly Arg Phe Arg Leu Leu Gly Asp Pro Ser 
65                  70                  75                  80  


Arg Asn Asn Cys Ser Leu Ser Ile Val Asp Ala Arg Arg Arg Asp Asn 
                85                  90                  95      


Gly Ser Tyr Phe Phe Arg Met Glu Arg Gly Ser Thr Lys Tyr Xaa Tyr 
            100                 105                 110         


Lys Ser Pro Gln Leu Ser Val His Val Thr Asp Leu Thr His Arg Pro 
        115                 120                 125             


Lys Ile Leu Ile Pro Gly Thr Leu Glu Pro Gly His Ser Lys Asn Leu 
    130                 135                 140                 


Thr Cys Ser Val Ser Trp Ala Cys Glu Gln Gly Thr Pro Pro Ile Phe 
145                 150                 155                 160 


Ser Trp Leu Ser Ala Ala Pro Thr Ser Leu Gly Pro Arg Thr Thr His 
                165                 170                 175     


Ser Ser Val Leu Ile Ile Thr Pro Arg Pro Gln Asp His Gly Thr Asn 
            180                 185                 190         


Leu Thr Cys Gln Val Lys Phe Ala Gly Ala Gly Val Thr Thr Glu Arg 
        195                 200                 205             


Thr Ile Gln Leu Asn Val Thr Tyr Val Pro Gln Asn Pro Thr Thr Gly 
    210                 215                 220                 


Ile Phe Pro Gly Asp Gly Ser Gly Lys Gln Glu Thr Arg Ala Gly Val 
225                 230                 235                 240 


Val His 
        


<210>  3
<211>  122
<212>  PRT
<213>  Homo sapiens

<400>  3

Pro Asn Phe Trp Leu Gln Val Gln Glu Ser Val Thr Val Gln Glu Gly 
1               5                   10                  15      


Leu Cys Val Leu Val Pro Cys Thr Phe Phe His Pro Ile Pro Tyr Tyr 
            20                  25                  30          


Asp Lys Asn Ser Pro Val His Gly Tyr Trp Phe Arg Glu Gly Ala Ile 
        35                  40                  45              


Ile Ser Arg Asp Ser Pro Val Ala Thr Asn Lys Leu Asp Gln Glu Val 
    50                  55                  60                  


Gln Glu Glu Thr Gln Gly Arg Phe Arg Leu Leu Gly Asp Pro Ser Arg 
65                  70                  75                  80  


Asn Asn Cys Ser Leu Ser Ile Val Asp Ala Arg Arg Arg Asp Asn Gly 
                85                  90                  95      


Ser Tyr Phe Phe Arg Met Glu Arg Gly Ser Thr Lys Tyr Ser Tyr Lys 
            100                 105                 110         


Ser Pro Gln Leu Ser Val His Val Thr Asp 
        115                 120         


<210>  4
<211>  122
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Trp or Arg

<220>
<221>  MISC_FEATURE
<222>  (51)..(51)
<223>  Xaa is Arg or Gly

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is Ser or Asn

<400>  4

Pro Asn Phe Xaa Leu Gln Val Gln Glu Ser Val Thr Val Gln Glu Gly 
1               5                   10                  15      


Leu Cys Val Leu Val Pro Cys Thr Phe Phe His Pro Ile Pro Tyr Tyr 
            20                  25                  30          


Asp Lys Asn Ser Pro Val His Gly Tyr Trp Phe Arg Glu Gly Ala Ile 
        35                  40                  45              


Ile Ser Xaa Asp Ser Pro Val Ala Thr Asn Lys Leu Asp Gln Glu Val 
    50                  55                  60                  


Gln Glu Glu Thr Gln Gly Arg Phe Arg Leu Leu Gly Asp Pro Ser Arg 
65                  70                  75                  80  


Asn Asn Cys Ser Leu Ser Ile Val Asp Ala Arg Arg Arg Asp Asn Gly 
                85                  90                  95      


Ser Tyr Phe Phe Arg Met Glu Arg Gly Ser Thr Lys Tyr Xaa Tyr Lys 
            100                 105                 110         


Ser Pro Gln Leu Ser Val His Val Thr Asp 
        115                 120         


<210>  5
<211>  608
<212>  PRT
<213>  Homo sapiens

<400>  5

Glu Ser Trp Glu Pro Cys Val Glu Val Val Pro Asn Ile Thr Tyr Gln 
1               5                   10                  15      


Cys Met Glu Leu Asn Phe Tyr Lys Ile Pro Asp Asn Leu Pro Phe Ser 
            20                  25                  30          


Thr Lys Asn Leu Asp Leu Ser Phe Asn Pro Leu Arg His Leu Gly Ser 
        35                  40                  45              


Tyr Ser Phe Phe Ser Phe Pro Glu Leu Gln Val Leu Asp Leu Ser Arg 
    50                  55                  60                  


Cys Glu Ile Gln Thr Ile Glu Asp Gly Ala Tyr Gln Ser Leu Ser His 
65                  70                  75                  80  


Leu Ser Thr Leu Ile Leu Thr Gly Asn Pro Ile Gln Ser Leu Ala Leu 
                85                  90                  95      


Gly Ala Phe Ser Gly Leu Ser Ser Leu Gln Lys Leu Val Ala Val Glu 
            100                 105                 110         


Thr Asn Leu Ala Ser Leu Glu Asn Phe Pro Ile Gly His Leu Lys Thr 
        115                 120                 125             


Leu Lys Glu Leu Asn Val Ala His Asn Leu Ile Gln Ser Phe Lys Leu 
    130                 135                 140                 


Pro Glu Tyr Phe Ser Asn Leu Thr Asn Leu Glu His Leu Asp Leu Ser 
145                 150                 155                 160 


Ser Asn Lys Ile Gln Ser Ile Tyr Cys Thr Asp Leu Arg Val Leu His 
                165                 170                 175     


Gln Met Pro Leu Leu Asn Leu Ser Leu Asp Leu Ser Leu Asn Pro Met 
            180                 185                 190         


Asn Phe Ile Gln Pro Gly Ala Phe Lys Glu Ile Arg Leu His Lys Leu 
        195                 200                 205             


Thr Leu Arg Asn Asn Phe Asp Ser Leu Asn Val Met Lys Thr Cys Ile 
    210                 215                 220                 


Gln Gly Leu Ala Gly Leu Glu Val His Arg Leu Val Leu Gly Glu Phe 
225                 230                 235                 240 


Arg Asn Glu Gly Asn Leu Glu Lys Phe Asp Lys Ser Ala Leu Glu Gly 
                245                 250                 255     


Leu Cys Asn Leu Thr Ile Glu Glu Phe Arg Leu Ala Tyr Leu Asp Tyr 
            260                 265                 270         


Tyr Leu Asp Asp Ile Ile Asp Leu Phe Asn Cys Leu Thr Asn Val Ser 
        275                 280                 285             


Ser Phe Ser Leu Val Ser Val Thr Ile Glu Arg Val Lys Asp Phe Ser 
    290                 295                 300                 


Tyr Asn Phe Gly Trp Gln His Leu Glu Leu Val Asn Cys Lys Phe Gly 
305                 310                 315                 320 


Gln Phe Pro Thr Leu Lys Leu Lys Ser Leu Lys Arg Leu Thr Phe Thr 
                325                 330                 335     


Ser Asn Lys Gly Gly Asn Ala Phe Ser Glu Val Asp Leu Pro Ser Leu 
            340                 345                 350         


Glu Phe Leu Asp Leu Ser Arg Asn Gly Leu Ser Phe Lys Gly Cys Cys 
        355                 360                 365             


Ser Gln Ser Asp Phe Gly Thr Thr Ser Leu Lys Tyr Leu Asp Leu Ser 
    370                 375                 380                 


Phe Asn Gly Val Ile Thr Met Ser Ser Asn Phe Leu Gly Leu Glu Gln 
385                 390                 395                 400 


Leu Glu His Leu Asp Phe Gln His Ser Asn Leu Lys Gln Met Ser Glu 
                405                 410                 415     


Phe Ser Val Phe Leu Ser Leu Arg Asn Leu Ile Tyr Leu Asp Ile Ser 
            420                 425                 430         


His Thr His Thr Arg Val Ala Phe Asn Gly Ile Phe Asn Gly Leu Ser 
        435                 440                 445             


Ser Leu Glu Val Leu Lys Met Ala Gly Asn Ser Phe Gln Glu Asn Phe 
    450                 455                 460                 


Leu Pro Asp Ile Phe Thr Glu Leu Arg Asn Leu Thr Phe Leu Asp Leu 
465                 470                 475                 480 


Ser Gln Cys Gln Leu Glu Gln Leu Ser Pro Thr Ala Phe Asn Ser Leu 
                485                 490                 495     


Ser Ser Leu Gln Val Leu Asn Met Ser His Asn Asn Phe Phe Ser Leu 
            500                 505                 510         


Asp Thr Phe Pro Tyr Lys Cys Leu Asn Ser Leu Gln Val Leu Asp Tyr 
        515                 520                 525             


Ser Leu Asn His Ile Met Thr Ser Lys Lys Gln Glu Leu Gln His Phe 
    530                 535                 540                 


Pro Ser Ser Leu Ala Phe Leu Asn Leu Thr Gln Asn Asp Phe Ala Cys 
545                 550                 555                 560 


Thr Cys Glu His Gln Ser Phe Leu Gln Trp Ile Lys Asp Gln Arg Gln 
                565                 570                 575     


Leu Leu Val Glu Val Glu Arg Met Glu Cys Ala Thr Pro Ser Asp Lys 
            580                 585                 590         


Gln Gly Met Pro Val Leu Ser Leu Asn Ile Thr Cys Gln Met Asn Lys 
        595                 600                 605             


<210>  6
<211>  320
<212>  PRT
<213>  Homo sapiens

<400>  6

Ala Gln Asn Ile Thr Ala Arg Ile Gly Glu Pro Leu Val Leu Lys Cys 
1               5                   10                  15      


Lys Gly Ala Pro Lys Lys Pro Pro Gln Arg Leu Glu Trp Lys Leu Asn 
            20                  25                  30          


Thr Gly Arg Thr Glu Ala Trp Lys Val Leu Ser Pro Gln Gly Gly Gly 
        35                  40                  45              


Pro Trp Asp Ser Val Ala Arg Val Leu Pro Asn Gly Ser Leu Phe Leu 
    50                  55                  60                  


Pro Ala Val Gly Ile Gln Asp Glu Gly Ile Phe Arg Cys Gln Ala Met 
65                  70                  75                  80  


Asn Arg Asn Gly Lys Glu Thr Lys Ser Asn Tyr Arg Val Arg Val Tyr 
                85                  90                  95      


Gln Ile Pro Gly Lys Pro Glu Ile Val Asp Ser Ala Ser Glu Leu Thr 
            100                 105                 110         


Ala Gly Val Pro Asn Lys Val Gly Thr Cys Val Ser Glu Gly Ser Tyr 
        115                 120                 125             


Pro Ala Gly Thr Leu Ser Trp His Leu Asp Gly Lys Pro Leu Val Pro 
    130                 135                 140                 


Asn Glu Lys Gly Val Ser Val Lys Glu Gln Thr Arg Arg His Pro Glu 
145                 150                 155                 160 


Thr Gly Leu Phe Thr Leu Gln Ser Glu Leu Met Val Thr Pro Ala Arg 
                165                 170                 175     


Gly Gly Asp Pro Arg Pro Thr Phe Ser Cys Ser Phe Ser Pro Gly Leu 
            180                 185                 190         


Pro Arg His Arg Ala Leu Arg Thr Ala Pro Ile Gln Pro Arg Val Trp 
        195                 200                 205             


Glu Pro Val Pro Leu Glu Glu Val Gln Leu Val Val Glu Pro Glu Gly 
    210                 215                 220                 


Gly Ala Val Ala Pro Gly Gly Thr Val Thr Leu Thr Cys Glu Val Pro 
225                 230                 235                 240 


Ala Gln Pro Ser Pro Gln Ile His Trp Met Lys Asp Gly Val Pro Leu 
                245                 250                 255     


Pro Leu Pro Pro Ser Pro Val Leu Ile Leu Pro Glu Ile Gly Pro Gln 
            260                 265                 270         


Asp Gln Gly Thr Tyr Ser Cys Val Ala Thr His Ser Ser His Gly Pro 
        275                 280                 285             


Gln Glu Ser Arg Ala Val Ser Ile Ser Ile Ile Glu Pro Gly Glu Glu 
    290                 295                 300                 


Gly Pro Thr Ala Gly Ser Val Gly Gly Ser Gly Leu Gly Thr Leu Ala 
305                 310                 315                 320 


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Asp Ile Glu Gly Arg Met Asp 
1               5           


