                         SEQUENCE LISTING

<110>  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
       CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
       INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE
       POMPON, Denis
       HONORINE, Romy
 
<120>  MTHODE D'INDUCTION D'UNE RECOMBINAISON MIOTIQUE CIBLE

<130>  VMA/ll-F539/163WO

<150>  FR1356664
<151>  2013-07-05

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  squence synthtique(NLS2)

<400>  1

Met Ala Ser Ser Pro Pro Lys Lys Lys Arg Lys Val Ser 
1               5                   10              


<210>  2
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 2

<400>  2

Trp Lys Asp Ala Ser Gly Ser Arg Met His Ala 
1               5                   10      


<210>  3
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  squence synthtique (TAL N)

<400>  3

Trp Ser Arg Met His Ala Asp Pro Ile Arg Pro Arg Arg Pro Ser Pro 
1               5                   10                  15      


Ala Arg Glu Leu Leu Pro Gly Pro Gln Pro Asp Arg Val Gln Pro Thr 
            20                  25                  30          


Ala Asp Arg Gly Val Ser Ala Pro Ala Gly Ser Pro Leu Asp Gly Leu 
        35                  40                  45              


Pro Ala Arg Arg Thr Val Ser Arg Thr Arg Leu Pro Ser Pro Pro Ala 
    50                  55                  60                  


Pro Ser Pro Ala Phe Ser Ala Gly Ser Phe Ser Asp Leu Leu Arg Pro 
65                  70                  75                  80  


Phe Asp Pro Ser Leu Leu Asp Thr Ser Leu Leu Asp Ser Met Pro Ala 
                85                  90                  95      


Val Gly Thr Pro His Thr Ala Ala Ala Pro Ala Glu Trp Asp Glu Ala 
            100                 105                 110         


Gln Ser Ala Leu Arg Ala Ala Asp Asp Pro Pro Pro Thr Val Arg Val 
        115                 120                 125             


Ala Val Thr Ala Ala Arg Pro Pro Arg Ala Lys Pro Ala Pro Arg Arg 
    130                 135                 140                 


Arg Ala Ala Gln Pro Ser Asp Ala Ser 
145                 150             


<210>  4
<211>  781
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  squence synthtique (TAL M)

<400>  4

Ala Ala Gln Val Asp Leu Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln 
1               5                   10                  15      


Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val Ala Gln His His Glu 
            20                  25                  30          


Ala Leu Val Gly His Gly Phe Thr His Ala His Ile Val Ala Leu Ser 
        35                  40                  45              


Gln His Pro Ala Ala Leu Gly Thr Val Ala Val Thr Tyr Gln His Ile 
    50                  55                  60                  


Ile Thr Ala Leu Pro Glu Ala Thr His Glu Asp Ile Val Gly Val Gly 
65                  70                  75                  80  


Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala 
                85                  90                  95      


Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp Thr Gly Gln Leu Val 
            100                 105                 110         


Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Met Glu Ala Val His Ala 
        115                 120                 125             


Ser Arg Asn Ala Leu Thr Gly Ala Pro Leu Glu Thr Leu Thr Pro Asp 
    130                 135                 140                 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
145                 150                 155                 160 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
                165                 170                 175     


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
            180                 185                 190         


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
        195                 200                 205             


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
    210                 215                 220                 


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
225                 230                 235                 240 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
                245                 250                 255     


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
            260                 265                 270         


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
        275                 280                 285             


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
    290                 295                 300                 


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
305                 310                 315                 320 


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
                325                 330                 335     


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
            340                 345                 350         


Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
        355                 360                 365             


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
    370                 375                 380                 


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
385                 390                 395                 400 


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
                405                 410                 415     


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
            420                 425                 430         


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
        435                 440                 445             


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala 
    450                 455                 460                 


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
465                 470                 475                 480 


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
                485                 490                 495     


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
            500                 505                 510         


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
        515                 520                 525             


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    530                 535                 540                 


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
545                 550                 555                 560 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                565                 570                 575     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            580                 585                 590         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        595                 600                 605             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    610                 615                 620                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
625                 630                 635                 640 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                645                 650                 655     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            660                 665                 670         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        675                 680                 685             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    690                 695                 700                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Ala Val 
705                 710                 715                 720 


Ser Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala 
                725                 730                 735     


Met Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg 
            740                 745                 750         


Arg Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Asp 
        755                 760                 765             


Tyr Ala Gln Val Val Arg Val Leu Glu Phe Phe Gln Cys 
    770                 775                 780     


<210>  5
<211>  154
<212>  PRT
<213>  Xanthomonas oryzae

<400>  5

His Ser His Pro Ala Tyr Ala Phe Asp Glu Ala Met Thr Gln Phe Gly 
1               5                   10                  15      


Met Ser Arg Asn Gly Leu Val Gln Leu Phe Arg Arg Val Gly Val Thr 
            20                  25                  30          


Glu Leu Glu Ala Arg Gly Gly Thr Leu Pro Pro Ala Ser Gln Arg Trp 
        35                  40                  45              


Asp Arg Ile Leu Gln Ala Ser Gly Met Lys Arg Ala Lys Pro Ser Pro 
    50                  55                  60                  


Thr Ser Ala Gln Thr Pro Asp Gln Ala Ser Leu His Ala Phe Ala Asp 
65                  70                  75                  80  


Ser Leu Glu Arg Asp Leu Asp Ala Pro Ser Pro Met His Glu Gly Asp 
                85                  90                  95      


Gln Thr Arg Ala Ser Ser Arg Lys Arg Ser Arg Ser Asp Arg Ala Val 
            100                 105                 110         


Thr Gly Pro Ser Ala Gln Gln Ala Val Glu Val Arg Val Pro Glu Gln 
        115                 120                 125             


Arg Asp Ala Leu His Leu Pro Leu Ser Trp Arg Val Lys Arg Pro Arg 
    130                 135                 140                 


Thr Arg Ile Trp Gly Gly Leu Pro Asp Pro 
145                 150                 


<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 3

<400>  6

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          


<210>  7
<211>  75
<212>  PRT
<213>  Clostridium thermocellum

<400>  7

Lys Val Pro Gly Thr Pro Ser Thr Lys Leu Tyr Gly Asp Val Asn Asp 
1               5                   10                  15      


Asp Gly Lys Val Asn Ser Thr Asp Ala Val Ala Leu Lys Arg Tyr Val 
            20                  25                  30          


Leu Arg Ser Gly Ile Ser Ile Asn Thr Asp Asn Ala Asp Leu Asn Glu 
        35                  40                  45              


Asp Gly Arg Val Asn Ser Thr Asp Leu Gly Ile Leu Lys Arg Tyr Ile 
    50                  55                  60                  


Leu Lys Glu Ile Asp Thr Leu Pro Tyr Lys Asn 
65                  70                  75  


<210>  8
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag2

<400>  8

Asp Tyr Lys Asp Asp Asp Asp Lys Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5                   10                  15      


Asp Tyr Lys Asp Asp Asp Asp Lys 
            20                  


<210>  9
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS1

<400>  9

Met Pro Pro Lys Lys Lys Arg Lys Val Ala Ser 
1               5                   10      


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag 1

<400>  10

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 1

<400>  11

Ser Arg Thr Ser 
1               


<210>  12
<211>  159
<212>  PRT
<213>  Clostridium thermocellum

<400>  12

Asp Gly Val Val Val Glu Ile Gly Lys Val Thr Gly Ser Val Gly Thr 
1               5                   10                  15      


Thr Val Glu Ile Pro Val Tyr Phe Arg Gly Val Pro Ser Lys Gly Ile 
            20                  25                  30          


Ala Asn Cys Asp Phe Val Phe Arg Tyr Asp Pro Asn Val Leu Glu Ile 
        35                  40                  45              


Ile Gly Ile Asp Pro Gly Asp Ile Ile Val Asp Pro Asn Pro Thr Lys 
    50                  55                  60                  


Ser Phe Asp Thr Ala Ile Tyr Pro Asp Arg Lys Ile Ile Val Phe Leu 
65                  70                  75                  80  


Phe Ala Glu Asp Ser Gly Thr Gly Ala Tyr Ala Ile Thr Lys Asp Gly 
                85                  90                  95      


Val Phe Ala Lys Ile Arg Ala Thr Val Lys Ser Ser Ala Pro Gly Tyr 
            100                 105                 110         


Ile Thr Phe Asp Glu Val Gly Gly Phe Ala Asp Asn Asp Leu Val Glu 
        115                 120                 125             


Gln Lys Val Ser Phe Ile Asp Gly Gly Val Asn Val Gly Asn Ala Thr 
    130                 135                 140                 


Pro Thr Lys Gly Ala Thr Pro Thr Asn Thr Ala Thr Pro Thr Lys 
145                 150                 155                 


<210>  13
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  squence synthtique (Spac 1)

<400>  13

Ser Ala Thr Ala Thr Pro Thr Arg Pro Ser Val Pro Thr Asn Thr Pro 
1               5                   10                  15      


Thr Asn Thr Pro Ala Asn Thr 
            20              


<210>  14
<211>  398
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  14

Met Ala Leu Glu Gly Leu Arg Lys Lys Tyr Lys Thr Arg Gln Glu Leu 
1               5                   10                  15      


Val Lys Ala Leu Thr Pro Lys Arg Arg Ser Ile His Leu Asn Ser Asn 
            20                  25                  30          


Gly His Ser Asn Gly Thr Pro Cys Ser Asn Ala Asp Val Leu Ala His 
        35                  40                  45              


Ile Lys His Phe Leu Ser Leu Ala Ala Asn Ser Leu Glu Gln His Gln 
    50                  55                  60                  


Gln Pro Ile Ser Ile Val Phe Gln Asn Lys Lys Lys Lys Gly Asp Thr 
65                  70                  75                  80  


Asn Ser Pro Asp Ile His Thr Thr Leu Asp Phe Pro Leu Asn Gly Pro 
                85                  90                  95      


His Leu Ser Thr His Gln Phe Lys Leu Lys Arg Cys Ala Ile Leu Leu 
            100                 105                 110         


Asn Leu Leu Lys Val Val Met Glu Lys Leu Pro Leu Gly Lys Asn Thr 
        115                 120                 125             


Thr Val Arg Asp Ile Phe Tyr Ser Asn Val Glu Leu Phe Gln Arg Gln 
    130                 135                 140                 


Ala Asn Val Val Gln Trp Leu Asp Val Ile Arg Phe Asn Phe Lys Leu 
145                 150                 155                 160 


Ser Pro Arg Lys Ser Leu Asn Ile Ile Pro Ala Gln Lys Gly Leu Val 
                165                 170                 175     


Tyr Ser Pro Phe Pro Ile Asp Ile Tyr Asp Asn Ile Leu Thr Cys Glu 
            180                 185                 190         


Asn Glu Pro Lys Met Gln Lys Gln Thr Ile Phe Ser Gly Lys Pro Cys 
        195                 200                 205             


Leu Ile Pro Phe Phe Gln Asp Asp Ala Val Ile Lys Leu Gly Thr Thr 
    210                 215                 220                 


Ser Met Cys Asn Ile Val Ile Val Glu Lys Glu Ala Val Phe Thr Lys 
225                 230                 235                 240 


Leu Val Asn Asn Tyr His Lys Leu Ser Thr Asn Thr Met Leu Ile Thr 
                245                 250                 255     


Gly Lys Gly Phe Pro Asp Phe Leu Thr Arg Leu Phe Leu Lys Lys Leu 
            260                 265                 270         


Glu Gln Tyr Cys Ser Asn Leu Ile Ser Asp Cys Ser Ile Phe Thr Asp 
        275                 280                 285             


Ala Asp Pro Tyr Gly Ile Ser Ile Ala Leu Asn Tyr Thr His Ser Asn 
    290                 295                 300                 


Glu Arg Asn Ala Tyr Ile Cys Thr Met Ala Asn Tyr Lys Gly Ile Arg 
305                 310                 315                 320 


Ile Thr Gln Val Leu Ala Gln Asn Asn Glu Val His Asn Lys Ser Ile 
                325                 330                 335     


Gln Leu Leu Ser Leu Asn Gln Arg Asp Tyr Ser Leu Ala Lys Asn Leu 
            340                 345                 350         


Ile Ala Ser Leu Thr Ala Asn Ser Trp Asp Ile Ala Thr Ser Pro Leu 
        355                 360                 365             


Lys Asn Val Val Ile Glu Cys Gln Arg Glu Ile Phe Phe Gln Lys Lys 
    370                 375                 380                 


Ala Glu Met Asn Glu Ile Asp Ala Gly Ile Phe Lys Tyr Lys 
385                 390                 395             


<210>  15
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificiel (T1D45)

<400>  15
tcctattgcg ttggctatcc gagtggatcc agcgaattgg tgccaccata gcggtatcgc       60

ctaatagcca acgcaatagg a                                                 81


<210>  16
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificiel (T1D25)

<400>  16
tcctattgcg ttggctatcc gagtggatcc agcgaattgg tgcatagcca acgcaatagg       60

a                                                                       61


<210>  17
<211>  28
<212>  DNA
<213>  Artificial Sequence (1M10)

<220>
<223>  squence artificielle (1M10)

<400>  17
tcctattgcg ttggctatcc gagtggat                                          28


