                         SEQUENCE LISTING

<110>  BIOCON Limited
 
<120>  SIGNAL SEQUENCE FOR PROTEIN EXPRESSION IN PICHIA PASTORIS

<130>  unknown

<160>  27    

<170>  PatentIn version 3.3

<210>  1
<211>  18
<212>  PRT
<213>  Pichia pastoris

<400>  1

Met Phe Asn Leu Lys Thr Ile Leu Ile Ser Thr Leu Ala Ser Ile Ala 
1               5                   10                  15      


Val Ala 
        


<210>  2
<211>  54
<212>  DNA
<213>  Pichia pastoris

<400>  2
atgttcaacc tgaaaactat tctcatctca acacttgcat cgatcgctgt tgcc           54


<210>  3
<211>  12
<212>  PRT
<213>  Pichia pastoris

<400>  3

Asp Gln Thr Phe Gly Val Leu Leu Ile Arg Ser Gly 
1               5                   10          


<210>  4
<211>  289
<212>  PRT
<213>  Pichia pastoris

<400>  4

Met Phe Asn Leu Lys Thr Ile Leu Ile Ser Thr Leu Ala Ser Ile Ala 
1               5                   10                  15      


Val Ala Asp Gln Thr Phe Gly Val Leu Leu Ile Arg Ser Gly Ser Pro 
            20                  25                  30          


Tyr His Tyr Ser Thr Leu Thr Asn Arg Asp Glu Lys Ile Val Ala Gly 
        35                  40                  45              


Gly Gly Asn Lys Lys Val Thr Leu Thr Asp Glu Gly Ala Leu Lys Tyr 
    50                  55                  60                  


Asp Gly Gly Lys Trp Ile Gly Leu Asp Asp Asp Gly Tyr Ala Val Gln 
65                  70                  75                  80  


Thr Asp Lys Pro Val Thr Gly Trp Ser Thr Asn Gly Gly Tyr Leu Tyr 
                85                  90                  95      


Phe Asp Gln Gly Leu Ile Val Cys Thr Glu Asp Tyr Ile Gly Tyr Val 
            100                 105                 110         


Lys Lys His Gly Glu Cys Lys Gly Asp Ser Tyr Gly Met Ala Trp Lys 
        115                 120                 125             


Val Leu Pro Ala Asp Asp Asp Lys Asp Asp Asp Lys Asp Asp Asp Lys 
    130                 135                 140                 


Asp Asp Asp Lys Asp Tyr Asp Asp Asp Asn Asp His Gly Asp Gly Asp 
145                 150                 155                 160 


Tyr Tyr Cys Ser Ile Thr Gly Thr Tyr Ala Ile Lys Ser Lys Gly Ser 
                165                 170                 175     


Lys His Gln Tyr Glu Ala Ile Lys Lys Val Asp Ala His Pro His Val 
            180                 185                 190         


Phe Ser Val Gly Gly Asp Gln Gly Asn Asp Leu Ile Val Thr Phe Gln 
        195                 200                 205             


Lys Asp Cys Ser Leu Val Asp Gln Asp Asn Arg Gly Tyr Val Tyr Asp 
    210                 215                 220                 


Pro Asn Ser Gly Glu Val Gly Asn Val Asp Pro Trp Gly Glu Leu Thr 
225                 230                 235                 240 


Pro Ser Val Lys Trp Asp Ile Asp Asp Gly Tyr Leu Ile Phe Asn Gly 
                245                 250                 255     


Glu Ser Asn Phe Arg Ser Cys Pro Ser Gly Asn Gly Tyr Ser Leu Ser 
            260                 265                 270         


Ile Lys Asp Cys Val Gly Gly Thr Asp Ile Gly Leu Lys Val Trp Glu 
        275                 280                 285             


Lys 
    


<210>  5
<211>  271
<212>  PRT
<213>  Pichia pastoris

<400>  5

Asp Gln Thr Phe Gly Val Leu Leu Ile Arg Ser Gly Ser Pro Tyr His 
1               5                   10                  15      


Tyr Ser Thr Leu Thr Asn Arg Asp Glu Lys Ile Val Ala Gly Gly Gly 
            20                  25                  30          


Asn Lys Lys Val Thr Leu Thr Asp Glu Gly Ala Leu Lys Tyr Asp Gly 
        35                  40                  45              


Gly Lys Trp Ile Gly Leu Asp Asp Asp Gly Tyr Ala Val Gln Thr Asp 
    50                  55                  60                  


Lys Pro Val Thr Gly Trp Ser Thr Asn Gly Gly Tyr Leu Tyr Phe Asp 
65                  70                  75                  80  


Gln Gly Leu Ile Val Cys Thr Glu Asp Tyr Ile Gly Tyr Val Lys Lys 
                85                  90                  95      


His Gly Glu Cys Lys Gly Asp Ser Tyr Gly Met Ala Trp Lys Val Leu 
            100                 105                 110         


Pro Ala Asp Asp Asp Lys Asp Asp Asp Lys Asp Asp Asp Lys Asp Asp 
        115                 120                 125             


Asp Lys Asp Tyr Asp Asp Asp Asn Asp His Gly Asp Gly Asp Tyr Tyr 
    130                 135                 140                 


Cys Ser Ile Thr Gly Thr Tyr Ala Ile Lys Ser Lys Gly Ser Lys His 
145                 150                 155                 160 


Gln Tyr Glu Ala Ile Lys Lys Val Asp Ala His Pro His Val Phe Ser 
                165                 170                 175     


Val Gly Gly Asp Gln Gly Asn Asp Leu Ile Val Thr Phe Gln Lys Asp 
            180                 185                 190         


Cys Ser Leu Val Asp Gln Asp Asn Arg Gly Tyr Val Tyr Asp Pro Asn 
        195                 200                 205             


Ser Gly Glu Val Gly Asn Val Asp Pro Trp Gly Glu Leu Thr Pro Ser 
    210                 215                 220                 


Val Lys Trp Asp Ile Asp Asp Gly Tyr Leu Ile Phe Asn Gly Glu Ser 
225                 230                 235                 240 


Asn Phe Arg Ser Cys Pro Ser Gly Asn Gly Tyr Ser Leu Ser Ile Lys 
                245                 250                 255     


Asp Cys Val Gly Gly Thr Asp Ile Gly Leu Lys Val Trp Glu Lys 
            260                 265                 270     


<210>  6
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for amplification of P. pastoris DDDK open reading
       frame.

<400>  6
cgggatccat gttcaacctg aaaactattc tc                                   32


<210>  7
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for amplification of P. pastoris DDDK open reading
       frame.

<400>  7
cggaattctt atttctccca tactttaagg ccaat                                35


<210>  8
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for amplification of a truncated DDDK open reading
       frame.

<400>  8
gaagatctga ccaaaccttc ggtgtcctt                                       29


<210>  9
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for amplifying the P. pastoris DDDK gene.

<400>  9
atcgatcgct gttgccaaaa gagaccaaac cttcggtgtc                           40


<210>  10
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for amplifying the P. pastoris DDDK signal 
       sequence.

<400>  10
ggatccatgt tcaacctgaa aac                                             23


<210>  11
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for amplifying a truncated P. pastoris DDDK signal
       seqeunce.

<400>  11
tattctcatc tcaacacttg catcgatcgc                                      30


<210>  12
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for amplifying a truncated P. pastoris DDDK signal
       seqeunce.

<400>  12
tgttgccctc gagcgg                                                     16


<210>  13
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for amplifying the P. pastoris DDDK signal 
       sequence

<400>  13
ccgctcgagg gcaacagcga tcgatgcaag                                      30


<210>  14
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for amplifying a truncated P. pastoris DDDK signal
       sequence.

<400>  14
tgttgagatg agaatagttt tcaggttga                                       29


<210>  15
<211>  14
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for amplifying a truncated P. pastoris DDDK signal
       sequence.

<400>  15
acatatggaa ttcc                                                       14


<210>  16
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for amplifying the CPB coding seqeunce.

<400>  16
ccatcgatcg ctgttgccga agctgaggca catcactc                             38


<210>  17
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for amplifying the ETI coding sequence.

<400>  17
ccatcgatcg ctgttgccgt tttgttggat ggtaatgg                             38


<210>  18
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  18
tgcccaactt gaactgagga                                                 20


<210>  19
<211>  946
<212>  DNA
<213>  Pichia pastoris

<400>  19
agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag     60

gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt    120

tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc    180

agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta    240

acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta    300

tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg    360

agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct    420

gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg    480

ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt    540

cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct    600

ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct    660

ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact    720

gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat    780

atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt    840

actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga    900

caacttgaga agatcaaaaa acaactaatt attcgaagga tccgat                   946


<210>  20
<211>  1221
<212>  DNA
<213>  Sus sp.

<400>  20
atggaagctg aggcacatca ctctggtgaa cattttgaag gagagaaagt tttcagagtt     60

aatgttgaag atgagaacga catttctttg cttcatgagt tggcctccac tagacaaatt    120

gacttctgga agccagattc agtcactcaa atcaaaccac actctaccgt tgactttaga    180

gttaaggctg aggatatttt ggctgttgag gactttctgg aacagaatga gttgcaatac    240

gaggttttga tcaacaattt gagatctgtt cttgaggctc aatttgactc tagagtcaga    300

acaactggac attcctacga gaagtataat aactgggaga ctattgaagc ctggactaaa    360

caagtcactt cagagaatcc tgatctgatt tctagaactg ccattggtac cacatttctg    420

ggtaacaata tctacttgct gaaagttggt aagcctggac ctaataagcc agctatcttt    480

atggattgtg gatttcatgc tagagagtgg atttctcatg cattctgtca atggtttgtt    540

agagaggctg ttttgactta tggatacgag tctcacatga ctgagtttct taacaagttg    600

gacttctacg ttttgccagt tttgaacatt gatggttaca tctacacttg gactaagaat    660

agaatgtgga gaaagactag atccactaat gctggtacaa cttgtattgg tactgatcca    720

aacagaaact ttgacgctgg atggtgtact accggtgcct caactgatcc atgtgacgag    780

acttactgtg gttctgctgc cgagtctgag aaagagacta aagctttggc tgactttatc    840

agaaataact tgtcatccat taaagcttac ttgactattc attcttattc tcaaatgatt    900

ttgtatccat actcctacga ttacaagttg cctgagaaca atgcagagtt gaataacctg    960

gctaaggctg ccgtcaaaga gcttgccact ttgtacggta ctaagtacac ttacggtcca   1020

ggtgctacta ccatttaccc agctgcaggt ggatctgatg actgggcata cgatcaaggt   1080

atcaagtact cttttacctt tgagttgaga gacaaaggta gatacggatt tatcttgcct   1140

gagtctcaaa ttcaagctac ttgtgaagag actatgttgg ctattaagta cgtcactaac   1200

tacgttttgg gacacttgta a                                             1221


<210>  21
<211>  406
<212>  PRT
<213>  Sus sp.

<400>  21

Met Glu Ala Glu Ala His His Ser Gly Glu His Phe Glu Gly Glu Lys 
1               5                   10                  15      


Val Phe Arg Val Asn Val Glu Asp Glu Asn Asp Ile Ser Leu Leu His 
            20                  25                  30          


Glu Leu Ala Ser Thr Arg Gln Ile Asp Phe Trp Lys Pro Asp Ser Val 
        35                  40                  45              


Thr Gln Ile Lys Pro His Ser Thr Val Asp Phe Arg Val Lys Ala Glu 
    50                  55                  60                  


Asp Ile Leu Ala Val Glu Asp Phe Leu Glu Gln Asn Glu Leu Gln Tyr 
65                  70                  75                  80  


Glu Val Leu Ile Asn Asn Leu Arg Ser Val Leu Glu Ala Gln Phe Asp 
                85                  90                  95      


Ser Arg Val Arg Thr Thr Gly His Ser Tyr Glu Lys Tyr Asn Asn Trp 
            100                 105                 110         


Glu Thr Ile Glu Ala Trp Thr Lys Gln Val Thr Ser Glu Asn Pro Asp 
        115                 120                 125             


Leu Ile Ser Arg Thr Ala Ile Gly Thr Thr Phe Leu Gly Asn Asn Ile 
    130                 135                 140                 


Tyr Leu Leu Lys Val Gly Lys Pro Gly Pro Asn Lys Pro Ala Ile Phe 
145                 150                 155                 160 


Met Asp Cys Gly Phe His Ala Arg Glu Trp Ile Ser His Ala Phe Cys 
                165                 170                 175     


Gln Trp Phe Val Arg Glu Ala Val Leu Thr Tyr Gly Tyr Glu Ser His 
            180                 185                 190         


Met Thr Glu Phe Leu Asn Lys Leu Asp Phe Tyr Val Leu Pro Val Leu 
        195                 200                 205             


Asn Ile Asp Gly Tyr Ile Tyr Thr Trp Thr Lys Asn Arg Met Trp Arg 
    210                 215                 220                 


Lys Thr Arg Ser Thr Asn Ala Gly Thr Thr Cys Ile Gly Thr Asp Pro 
225                 230                 235                 240 


Asn Arg Asn Phe Asp Ala Gly Trp Cys Thr Thr Gly Ala Ser Thr Asp 
                245                 250                 255     


Pro Cys Asp Glu Thr Tyr Cys Gly Ser Ala Ala Glu Ser Glu Lys Glu 
            260                 265                 270         


Thr Lys Ala Leu Ala Asp Phe Ile Arg Asn Asn Leu Ser Ser Ile Lys 
        275                 280                 285             


Ala Tyr Leu Thr Ile His Ser Tyr Ser Gln Met Ile Leu Tyr Pro Tyr 
    290                 295                 300                 


Ser Tyr Asp Tyr Lys Leu Pro Glu Asn Asn Ala Glu Leu Asn Asn Leu 
305                 310                 315                 320 


Ala Lys Ala Ala Val Lys Glu Leu Ala Thr Leu Tyr Gly Thr Lys Tyr 
                325                 330                 335     


Thr Tyr Gly Pro Gly Ala Thr Thr Ile Tyr Pro Ala Ala Gly Gly Ser 
            340                 345                 350         


Asp Asp Trp Ala Tyr Asp Gln Gly Ile Lys Tyr Ser Phe Thr Phe Glu 
        355                 360                 365             


Leu Arg Asp Lys Gly Arg Tyr Gly Phe Ile Leu Pro Glu Ser Gln Ile 
    370                 375                 380                 


Gln Ala Thr Cys Glu Glu Thr Met Leu Ala Ile Lys Tyr Val Thr Asn 
385                 390                 395                 400 


Tyr Val Leu Gly His Leu 
                405     


<210>  22
<211>  1272
<212>  DNA
<213>  Artificial

<220>
<223>  coding sequence for porcine CPB comprising the P. pastoris DDDK 
       signal sequence.

<400>  22
atgttcaacc tgaaaactat tctcatctca acacttgcat cgatcgctgt tgccgaagct     60

gaggcacatc actctggtga acattttgaa ggagagaaag ttttcagagt taatgttgaa    120

gatgagaacg acatttcttt gcttcatgag ttggcctcca ctagacaaat tgacttctgg    180

aagccagatt cagtcactca aatcaaacca cactctaccg ttgactttag agttaaggct    240

gaggatattt tggctgttga ggactttctg gaacagaatg agttgcaata cgaggttttg    300

atcaacaatt tgagatctgt tcttgaggct caatttgact ctagagtcag aacaactgga    360

cattcctacg agaagtataa taactgggag actattgaag cctggactaa acaagtcact    420

tcagagaatc ctgatctgat ttctagaact gccattggta ccacatttct gggtaacaat    480

atctacttgc tgaaagttgg taagcctgga cctaataagc cagctatctt tatggattgt    540

ggatttcatg ctagagagtg gatttctcat gcattctgtc aatggtttgt tagagaggct    600

gttttgactt atggatacga gtctcacatg actgagtttc ttaacaagtt ggacttctac    660

gttttgccag ttttgaacat tgatggttac atctacactt ggactaagaa tagaatgtgg    720

agaaagacta gatccactaa tgctggtaca acttgtattg gtactgatcc aaacagaaac    780

tttgacgctg gatggtgtac taccggtgcc tcaactgatc catgtgacga gacttactgt    840

ggttctgctg ccgagtctga gaaagagact aaagctttgg ctgactttat cagaaataac    900

ttgtcatcca ttaaagctta cttgactatt cattcttatt ctcaaatgat tttgtatcca    960

tactcctacg attacaagtt gcctgagaac aatgcagagt tgaataacct ggctaaggct   1020

gccgtcaaag agcttgccac tttgtacggt actaagtaca cttacggtcc aggtgctact   1080

accatttacc cagctgcagg tggatctgat gactgggcat acgatcaagg tatcaagtac   1140

tcttttacct ttgagttgag agacaaaggt agatacggat ttatcttgcc tgagtctcaa   1200

attcaagcta cttgtgaaga gactatgttg gctattaagt acgtcactaa ctacgttttg   1260

ggacacttgt aa                                                       1272


<210>  23
<211>  423
<212>  PRT
<213>  Artificial

<220>
<223>  porcine carboxypeptidase B comprising the P. pastoris DDDK signal
       sequence.

<400>  23

Met Phe Asn Leu Lys Thr Ile Leu Ile Ser Thr Leu Ala Ser Ile Ala 
1               5                   10                  15      


Val Ala Glu Ala Glu Ala His His Ser Gly Glu His Phe Glu Gly Glu 
            20                  25                  30          


Lys Val Phe Arg Val Asn Val Glu Asp Glu Asn Asp Ile Ser Leu Leu 
        35                  40                  45              


His Glu Leu Ala Ser Thr Arg Gln Ile Asp Phe Trp Lys Pro Asp Ser 
    50                  55                  60                  


Val Thr Gln Ile Lys Pro His Ser Thr Val Asp Phe Arg Val Lys Ala 
65                  70                  75                  80  


Glu Asp Ile Leu Ala Val Glu Asp Phe Leu Glu Gln Asn Glu Leu Gln 
                85                  90                  95      


Tyr Glu Val Leu Ile Asn Asn Leu Arg Ser Val Leu Glu Ala Gln Phe 
            100                 105                 110         


Asp Ser Arg Val Arg Thr Thr Gly His Ser Tyr Glu Lys Tyr Asn Asn 
        115                 120                 125             


Trp Glu Thr Ile Glu Ala Trp Thr Lys Gln Val Thr Ser Glu Asn Pro 
    130                 135                 140                 


Asp Leu Ile Ser Arg Thr Ala Ile Gly Thr Thr Phe Leu Gly Asn Asn 
145                 150                 155                 160 


Ile Tyr Leu Leu Lys Val Gly Lys Pro Gly Pro Asn Lys Pro Ala Ile 
                165                 170                 175     


Phe Met Asp Cys Gly Phe His Ala Arg Glu Trp Ile Ser His Ala Phe 
            180                 185                 190         


Cys Gln Trp Phe Val Arg Glu Ala Val Leu Thr Tyr Gly Tyr Glu Ser 
        195                 200                 205             


His Met Thr Glu Phe Leu Asn Lys Leu Asp Phe Tyr Val Leu Pro Val 
    210                 215                 220                 


Leu Asn Ile Asp Gly Tyr Ile Tyr Thr Trp Thr Lys Asn Arg Met Trp 
225                 230                 235                 240 


Arg Lys Thr Arg Ser Thr Asn Ala Gly Thr Thr Cys Ile Gly Thr Asp 
                245                 250                 255     


Pro Asn Arg Asn Phe Asp Ala Gly Trp Cys Thr Thr Gly Ala Ser Thr 
            260                 265                 270         


Asp Pro Cys Asp Glu Thr Tyr Cys Gly Ser Ala Ala Glu Ser Glu Lys 
        275                 280                 285             


Glu Thr Lys Ala Leu Ala Asp Phe Ile Arg Asn Asn Leu Ser Ser Ile 
    290                 295                 300                 


Lys Ala Tyr Leu Thr Ile His Ser Tyr Ser Gln Met Ile Leu Tyr Pro 
305                 310                 315                 320 


Tyr Ser Tyr Asp Tyr Lys Leu Pro Glu Asn Asn Ala Glu Leu Asn Asn 
                325                 330                 335     


Leu Ala Lys Ala Ala Val Lys Glu Leu Ala Thr Leu Tyr Gly Thr Lys 
            340                 345                 350         


Tyr Thr Tyr Gly Pro Gly Ala Thr Thr Ile Tyr Pro Ala Ala Gly Gly 
        355                 360                 365             


Ser Asp Asp Trp Ala Tyr Asp Gln Gly Ile Lys Tyr Ser Phe Thr Phe 
    370                 375                 380                 


Glu Leu Arg Asp Lys Gly Arg Tyr Gly Phe Ile Leu Pro Glu Ser Gln 
385                 390                 395                 400 


Ile Gln Ala Thr Cys Glu Glu Thr Met Leu Ala Ile Lys Tyr Val Thr 
                405                 410                 415     


Asn Tyr Val Leu Gly His Leu 
            420             


<210>  24
<211>  522
<212>  DNA
<213>  Erythrina sp.

<400>  24
atggttttgt tggatggtaa tggtgaagtt gttcaaaatg gtggtactta ttatttgttg     60

ccacaagttt gggctcaagg tggtggtgtt caattggcta agactggtga agaaacttgt    120

ccattgactg ttgttcaatc tccaaatgaa ttgtctgatg gtaagccaat tagaattgaa    180

tctagattga gatctgcttt tattccagat gatgataagg ttagaattgg ttttgcttat    240

gctccaaagt gtgctccatc tccatggtgg actgttgttg aagatgaaca agaaggtttg    300

tctgttaagt tgtctgaaga tgaatctact caatttgatt atccatttaa gtttgaacaa    360

gtttctgatc aattgcattc ttataagttg ttgtattgtg aaggtaagca tgaaaagtgt    420

gcttctattg gtattaatag agatcaaaag ggttatagaa gattggttgt tactgaagat    480

tatccattga ctgttgtttt gaagaaggat gaatcttctt aa                       522


<210>  25
<211>  173
<212>  PRT
<213>  Erythrina sp.

<400>  25

Met Val Leu Leu Asp Gly Asn Gly Glu Val Val Gln Asn Gly Gly Thr 
1               5                   10                  15      


Tyr Tyr Leu Leu Pro Gln Val Trp Ala Gln Gly Gly Gly Val Gln Leu 
            20                  25                  30          


Ala Lys Thr Gly Glu Glu Thr Cys Pro Leu Thr Val Val Gln Ser Pro 
        35                  40                  45              


Asn Glu Leu Ser Asp Gly Lys Pro Ile Arg Ile Glu Ser Arg Leu Arg 
    50                  55                  60                  


Ser Ala Phe Ile Pro Asp Asp Asp Lys Val Arg Ile Gly Phe Ala Tyr 
65                  70                  75                  80  


Ala Pro Lys Cys Ala Pro Ser Pro Trp Trp Thr Val Val Glu Asp Glu 
                85                  90                  95      


Gln Glu Gly Leu Ser Val Lys Leu Ser Glu Asp Glu Ser Thr Gln Phe 
            100                 105                 110         


Asp Tyr Pro Phe Lys Phe Glu Gln Val Ser Asp Gln Leu His Ser Tyr 
        115                 120                 125             


Lys Leu Leu Tyr Cys Glu Gly Lys His Glu Lys Cys Ala Ser Ile Gly 
    130                 135                 140                 


Ile Asn Arg Asp Gln Lys Gly Tyr Arg Arg Leu Val Val Thr Glu Asp 
145                 150                 155                 160 


Tyr Pro Leu Thr Val Val Leu Lys Lys Asp Glu Ser Ser 
                165                 170             


<210>  26
<211>  573
<212>  DNA
<213>  Artificial

<220>
<223>  coding sequence for Erythrina Trypsin Inhibito comprising the P. 
       pastoris DDDK signal sequence.

<400>  26
atgttcaacc tgaaaactat tctcatctca acacttgcat cgatcgctgt tgccgttttg     60

ttggatggta atggtgaagt tgttcaaaat ggtggtactt attatttgtt gccacaagtt    120

tgggctcaag gtggtggtgt tcaattggct aagactggtg aagaaacttg tccattgact    180

gttgttcaat ctccaaatga attgtctgat ggtaagccaa ttagaattga atctagattg    240

agatctgctt ttattccaga tgatgataag gttagaattg gttttgctta tgctccaaag    300

tgtgctccat ctccatggtg gactgttgtt gaagatgaac aagaaggttt gtctgttaag    360

ttgtctgaag atgaatctac tcaatttgat tatccattta agtttgaaca agtttctgat    420

caattgcatt cttataagtt gttgtattgt gaaggtaagc atgaaaagtg tgcttctatt    480

ggtattaata gagatcaaaa gggttataga agattggttg ttactgaaga ttatccattg    540

actgttgttt tgaagaagga tgaatcttct taa                                 573


<210>  27
<211>  537
<212>  PRT
<213>  Artificial

<220>
<223>  Erythrina Trypsin Inhibitor comprising the P. pastoris DDDK 
       signal sequence.

<400>  27

Met Phe Asn Leu Lys Thr Ile Leu Ile Ser Thr Leu Ala Ser Ile Ala 
1               5                   10                  15      


Val Ala Gly Thr Thr Thr Thr Gly Thr Thr Gly Gly Ala Thr Gly Gly 
            20                  25                  30          


Thr Ala Ala Thr Gly Gly Thr Gly Ala Ala Gly Thr Thr Gly Thr Thr 
        35                  40                  45              


Cys Ala Ala Ala Ala Thr Gly Gly Thr Gly Gly Thr Ala Cys Thr Thr 
    50                  55                  60                  


Ala Thr Thr Ala Thr Thr Thr Gly Thr Thr Gly Cys Cys Ala Cys Ala 
65                  70                  75                  80  


Ala Gly Thr Thr Thr Gly Gly Gly Cys Thr Cys Ala Ala Gly Gly Thr 
                85                  90                  95      


Gly Gly Thr Gly Gly Thr Gly Thr Thr Cys Ala Ala Thr Thr Gly Gly 
            100                 105                 110         


Cys Thr Ala Ala Gly Ala Cys Thr Gly Gly Thr Gly Ala Ala Gly Ala 
        115                 120                 125             


Ala Ala Cys Thr Thr Gly Thr Cys Cys Ala Thr Thr Gly Ala Cys Thr 
    130                 135                 140                 


Gly Thr Thr Gly Thr Thr Cys Ala Ala Thr Cys Thr Cys Cys Ala Ala 
145                 150                 155                 160 


Ala Thr Gly Ala Ala Thr Thr Gly Thr Cys Thr Gly Ala Thr Gly Gly 
                165                 170                 175     


Thr Ala Ala Gly Cys Cys Ala Ala Thr Thr Ala Gly Ala Ala Thr Thr 
            180                 185                 190         


Gly Ala Ala Thr Cys Thr Ala Gly Ala Thr Thr Gly Ala Gly Ala Thr 
        195                 200                 205             


Cys Thr Gly Cys Thr Thr Thr Thr Ala Thr Thr Cys Cys Ala Gly Ala 
    210                 215                 220                 


Thr Gly Ala Thr Gly Ala Thr Ala Ala Gly Gly Thr Thr Ala Gly Ala 
225                 230                 235                 240 


Ala Thr Thr Gly Gly Thr Thr Thr Thr Gly Cys Thr Thr Ala Thr Gly 
                245                 250                 255     


Cys Thr Cys Cys Ala Ala Ala Gly Thr Gly Thr Gly Cys Thr Cys Cys 
            260                 265                 270         


Ala Thr Cys Thr Cys Cys Ala Thr Gly Gly Thr Gly Gly Ala Cys Thr 
        275                 280                 285             


Gly Thr Thr Gly Thr Thr Gly Ala Ala Gly Ala Thr Gly Ala Ala Cys 
    290                 295                 300                 


Ala Ala Gly Ala Ala Gly Gly Thr Thr Thr Gly Thr Cys Thr Gly Thr 
305                 310                 315                 320 


Thr Ala Ala Gly Thr Thr Gly Thr Cys Thr Gly Ala Ala Gly Ala Thr 
                325                 330                 335     


Gly Ala Ala Thr Cys Thr Ala Cys Thr Cys Ala Ala Thr Thr Thr Gly 
            340                 345                 350         


Ala Thr Thr Ala Thr Cys Cys Ala Thr Thr Thr Ala Ala Gly Thr Thr 
        355                 360                 365             


Thr Gly Ala Ala Cys Ala Ala Gly Thr Thr Thr Cys Thr Gly Ala Thr 
    370                 375                 380                 


Cys Ala Ala Thr Thr Gly Cys Ala Thr Thr Cys Thr Thr Ala Thr Ala 
385                 390                 395                 400 


Ala Gly Thr Thr Gly Thr Thr Gly Thr Ala Thr Thr Gly Thr Gly Ala 
                405                 410                 415     


Ala Gly Gly Thr Ala Ala Gly Cys Ala Thr Gly Ala Ala Ala Ala Gly 
            420                 425                 430         


Thr Gly Thr Gly Cys Thr Thr Cys Thr Ala Thr Thr Gly Gly Thr Ala 
        435                 440                 445             


Thr Thr Ala Ala Thr Ala Gly Ala Gly Ala Thr Cys Ala Ala Ala Ala 
    450                 455                 460                 


Gly Gly Gly Thr Thr Ala Thr Ala Gly Ala Ala Gly Ala Thr Thr Gly 
465                 470                 475                 480 


Gly Thr Thr Gly Thr Thr Ala Cys Thr Gly Ala Ala Gly Ala Thr Thr 
                485                 490                 495     


Ala Thr Cys Cys Ala Thr Thr Gly Ala Cys Thr Gly Thr Thr Gly Thr 
            500                 505                 510         


Thr Thr Thr Gly Ala Ala Gly Ala Ala Gly Gly Ala Thr Gly Ala Ala 
        515                 520                 525             


Thr Cys Thr Thr Cys Thr Thr Ala Ala 
    530                 535         


