                         SEQUENCE LISTING

<110>  Universitat de Barcelona
       Fundaci Institut de Recerca Biomdica (IRB Barcelona)
 
<120>  Actively transported and protease-resistant peptides as BBB 
       shuttles and shuttle-cargo constructs

<130>  WO-AVCRI220E

<150>  EP13382274.2
<151>  2013-07-04

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is an amino acid selected form C, D, E, K, O (Ornithine), Dap
       (diaminopropionic acid), Dab (diaminobutyric acid), Sec 
       (selenocystein) , Pen (penicillamine), AlGly (allyl Glycine) and 
       alpha-Me-alpha-alkGly (alpha-methyl, alpha-alkenylglycine)

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is an amino acid selected form C, D, E, K, O (Ornithine), Dap
       (diaminopropionic acid), Dab (diaminobutyric acid), Sec 
       (selenocystein) , Pen (penicillamine), AlGly (allyl Glycine) and 
       alpha-Me-alpha-alkGly (alpha-methyl, alpha-alkenylglycine)

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<400>  1

Xaa Lys Ala Pro Glu Thr Ala Leu Xaa Ala Ala Ala 
1               5                   10          


<210>  2
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<400>  2

Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala Ala 
1               5                   10          


<210>  3
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Alanine (A) amino acid can be repeated n times, wherein n is an 
       integer independently selected from 0 and 1

<400>  3

Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala 
1               5                   10      


<210>  4
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle

<400>  4

Cys Lys Ala Pro Glu Thr Ala Leu Cys 
1               5                   


<210>  5
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle

<400>  5

Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala 
1               5                   10  


<210>  6
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle

<400>  6

Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala 
1               5                   10      


<210>  7
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle

<400>  7

Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala Ala 
1               5                   10          


<210>  8
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is an amino acid selected from C, Sec (selenocystein), Pen 
       (penicillamine), Dab (diaminobutyric acid), Dap (diaminopropionic
       acid), K, O (Ornithine), D, E, AlGly (allyl Glycine) and 
       alpha-Me-alpha-alkGly (alpha-methyl, alpha-alkenylglycine)

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is an amino acid selected from the group consisting of H, N, 
       L, A, G, F, Y, W, I, V, P, S, T, D, E, K, and M.

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa is an amino acid selected from C, Sec (selenocystein), Pen 
       (penicillamine), Dab (diaminobutyric acid), Dap (diaminopropionic
       acid), K, O (Ornithine), D, E, AlGly (allyl Glycine) and 
       alpha-Me-alpha-alkGly (alpha-methyl, alpha-alkenylglycine).

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa is an amino acid selected from the group consisting in 
       hystidine (H), G,F Y, W, I, V, P, N, L, A, S, T, D, E, K and M.

<400>  8

Xaa Xaa Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala Ala Xaa Xaa 
1               5                   10                  15      


<210>  9
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle

<400>  9

Cys Asn Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala Ala Cys His 
1               5                   10                  15      


<210>  10
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle

<400>  10

Lys Ala Pro Glu Thr Ala Leu 
1               5           


<210>  11
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Drug

<400>  11

Leu Pro Phe Phe Asp 
1               5   


<210>  12
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  BBB shuttle


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is the amino acid 2,3-diaminopropionic acid (Dap)

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is the amino acid diaminopropionic acid (Dap)

<400>  12

Xaa Lys Ala Pro Glu Thr Ala Leu Asp 
1               5                   


<210>  13
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  construct

<400>  13

Leu Pro Phe Phe Asp Gly Cys Asn Cys Lys Ala Pro Glu Thr Ala Leu 
1               5                   10                  15      


Cys Ala Ala Ala Cys His 
            20          


<210>  14
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  construct

<400>  14

Cys Asn Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala Ala Cys His 
1               5                   10                  15      


Gly Leu Pro Phe Phe Asp 
            20          


<210>  15
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  construct

<400>  15

Leu Pro Phe Phe Asp Gly Cys Lys Ala Pro Glu Thr Ala Leu Cys 
1               5                   10                  15  


<210>  16
<211>  18
<212>  PRT
<213>  Apis mellifera

<400>  16

Cys Asn Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Arg Arg Cys Gln 
1               5                   10                  15      


Gln His 
        


<210>  17
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BBB shuttle


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is an amino acid selected from the group consisting of H, N, 
       L, A, G, F, Y, W, I, V, P, S, T, D, E, K, and M.

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa is an amino acid selected from the group consisting of H, 
       G,F, Y, W, I, V, P, N, L, A, S, T, D, E, K and M

<400>  17

Cys Xaa Cys Lys Ala Pro Glu Thr Ala Leu Cys Ala Ala Ala Cys Xaa 
1               5                   10                  15      


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BBB shuttle


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is the amino acid diaminopropionic acid (Dap)

<400>  18

Cys Xaa Lys Ala Pro Glu Thr Ala Leu Asp 
1               5                   10  


<210>  19
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is the amino acid diaminopropionic acid (Dap)

<400>  19

Xaa Lys Ala Pro Glu Thr Ala Leu Asp Lys Gln Ile Glu Ile Lys Lys 
1               5                   10                  15      


Phe Lys 
        


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  drug

<400>  20

Lys Gln Ile Glu Ile Lys Lys Phe Lys 
1               5                   


