                               SEQUENCE LISTING                               

<110> Aeras Global TB Vaccine Foundation

<120> Tuberculosis Compositions And Methods Of Using The Same

<130> 139268.01402 WO

<140> 
<141> 

<150> 61/838,872
<151> 2013-06-25

<160> 98

<170> PatentIn Ver. 2.1

<210> 1
<211> 977
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Ag85B 

<400> 1
atgacagacg tgagccgaaa gattcgagct tggggacgcc gattgatgat cggcacggca 60

gcggctgtag tccttccggg cctggtgggg cttgccgcgg agcggcaacc gcgggcgcgt 120

tctcccggcc ggggctgccg gtcgagtacc tgcaggtgcc gtcgccgtcg atgggccgcg 180

acatcaaggt tcagttccag agcggtggga acaactcacc tgcggtttat ctgctcgacg 240

gcctgcgcgc ccaagacgac tacaacggct gggatatcaa caccccggcg ttcgagtggt 300

actaccagtc gggactgtcg atagtcatgc cggtcggcgg gcagtccagc ttctacagcg 360

actggtacag cccggcctgc ggtaaggctg gctgccagac ttacaagtgg gaaaccttcc 420

tgaccagcga gctgccgcaa tggttgtccg ccaacagggc cgtgaagccc accggcagcg 480

ctgcaatcgg cttgtcgatg gccggctcgt cggcaatgat cttggccgcc taccaccccc 540

agcagttcat ctacgccggc tcgctgtcgg ccctgctgga cccctctcag gggatggggc 600

ctagcctgat cggcctcgcg atgggtgacg ccggcggtta caaggccgca gacatgtggg 660

gtccctcgag tgacccggca tgggagcgca acgaccctac gcagcagatc cccaagctgg 720

tcgcaaacaa cacccggcta tgggtttatt gcgggaacgg caccccgaac gagttgggcg 780

gtgccaacat acccgccgag ttcttggaga acttcgttcg tagcagcaac ctgaagttcc 840

aggatgcgta caacgccgcg ggcgggcaca acgccgtgtt caacttcccg cccaacggca 900

cgcacagctg ggagtactgg ggcgctcagc tcaacgccat gaagggtgac ctgcagagtt 960

cgttaggcgc cggctga                                                977


<210> 2
<211> 858
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Ag85B (E. coli 
      optimized) 

<400> 2
atgtttagcc gtcctggcct gccagttgaa tacctgcaag ttccgagccc gtccatgggt 60

cgtgacatta aggtgcagtt ccagagcggc ggtaacaata gcccggctgt gtacctgctg 120

gacggtctgc gtgcgcagga tgattacaac ggctgggaca tcaatacccc ggcatttgag 180

tggtattacc agtcgggtct gagcattgtg atgccggttg gcggtcaaag cagcttctat 240

agcgattggt acagcccggc atgcggcaag gctggttgcc aaacctacaa gtgggaaact 300

ttcttgacca gcgagctgcc gcaatggttg agcgccaacc gtgcggtcaa accgaccggt 360

agcgctgcta ttggcctgtc catggccggc agcagcgcga tgatcttggc ggcataccat 420

ccgcagcagt ttatctacgc cggtagcctg agcgcattgc tggacccgag ccaaggcatg 480

ggtccgagcc tgattggtct ggcaatgggt gacgcaggtg gttacaaagc ggccgatatg 540

tggggcccat ctagcgaccc ggcatgggag cgtaatgacc cgacccagca aattccgaaa 600

ctggtggcga ataacacgcg cctgtgggtc tactgtggca atggtacgcc gaacgagctg 660

ggtggcgcga atatccctgc ggagtttctg gaaaactttg ttcgcagcag caacctgaaa 720

ttccaggacg cgtataacgc agccggtggt cacaatgcgg ttttcaattt cccgccaaat 780

ggcactcata gctgggagta ctggggtgcg cagttgaacg caatgaaagg cgatctgcaa 840

tcctctctgg gtgcgggc                                               858


<210> 3
<211> 858
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Ag85B (human 
      optimized) 

<400> 3
atgttctcca ggcccggcct gcctgtcgag tatctgcagg tcccctcccc ctccatgggc 60

agagacatca aggtgcagtt ccaatccgga ggcaacaaca gccccgccgt gtatctcctc 120

gacggcctga gggctcagga cgactacaac ggctgggaca tcaacacccc cgccttcgag 180

tggtactacc agtccggact gagcatcgtc atgcccgtgg gcggccagag ctccttctac 240

agcgactggt atagccctgc ctgcggcaaa gccggatgcc agacctacaa gtgggagacc 300

tttctgacca gcgaactgcc ccagtggctg tccgccaata gggccgtcaa acctaccggc 360

tccgctgcca tcggactcag catggccgga agctccgcta tgatcctggc cgcctaccac 420

ccccagcaat ttatctacgc tggcagcctg tccgctctgc tggatcctag ccaaggcatg 480

ggccctagcc tcattggcct ggccatgggc gatgctggcg gctataaggc cgccgatatg 540

tggggcccta gctccgatcc tgcctgggag aggaatgacc ccacccagca gatccccaag 600

ctggtggcca acaacacaag gctctgggtg tactgcggca atggcacccc caacgaactg 660

ggcggagcca acattcccgc cgagttcctg gagaacttcg tcaggagcag caacctgaag 720

ttccaggacg cctacaatgc cgccggaggc cacaacgctg tgttcaactt ccctcccaac 780

ggcacccaca gctgggagta ttggggcgct cagctgaacg ccatgaaagg cgacctccag 840

agctccctgg gagctgga                                               858


<210> 4
<211> 325
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Ag85B 

<400> 4
Met Thr Asp Val Ser Arg Lys Ile Arg Ala Trp Gly Arg Arg Leu Met
1               5                   10                  15

Ile Gly Thr Ala Ala Ala Val Val Leu Pro Gly Leu Val Gly Leu Ala 
            20                  25                  30

Gly Gly Ala Ala Thr Ala Gly Ala Phe Ser Arg Pro Gly Leu Pro Val 
        35                  40                  45

Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp Ile Lys Val 
    50                  55                  60

Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr Leu Leu Asp 
65                  70                  75                  80

Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile Asn Thr Pro 
                85                  90                  95

Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val Met Pro Val 
            100                 105                 110

Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro Ala Cys Gly 
        115                 120                 125

Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu Thr Ser Glu
    130                 135                 140

Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro Thr Gly Ser 
145                 150                 155                 160

Ala Ala Ile Gly Leu Ser Met Ala Gly Ser Ser Ala Met Ile Leu Ala 
                165                 170                 175

Ala Tyr His Pro Gln Gln Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu
            180                 185                 190

Leu Asp Pro Ser Gln Gly Met Gly Pro Ser Leu Ile Gly Leu Ala Met 
        195                 200                 205

Gly Asp Ala Gly Gly Tyr Lys Ala Ala Asp Met Trp Gly Pro Ser Ser 
    210                 215                 220

Asp Pro Ala Trp Glu Arg Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu 
225                 230                 235                 240

Val Ala Asn Asn Thr Arg Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro 
                245                 250                 255

Asn Glu Leu Gly Gly Ala Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe 
            260                 265                 270

Val Arg Ser Ser Asn Leu Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly 
        275                 280                 285

Gly His Asn Ala Val Phe Asn Phe Pro Pro Asn Gly Thr His Ser Trp 
    290                 295                 300

Glu Tyr Trp Gly Ala Gln Leu Asn Ala Met Lys Gly Asp Leu Gln Ser 
305                 310                 315                 320

Ser Leu Gly Ala Gly
                325

<210> 5
<211> 286
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Ag85B (E. coli 
      and human optimized)

<400> 5
Met Phe Ser Arg Pro Gly Leu Pro Val Glu Tyr Leu Gln Val Pro Ser
1               5                   10                  15

Pro Ser Met Gly Arg Asp Ile Lys Val Gln Phe Gln Ser Gly Gly Asn
            20                  25                  30

Asn Ser Pro Ala Val Tyr Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp
        35                  40                  45

Tyr Asn Gly Trp Asp Ile Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln  
    50                  55                  60

Ser Gly Leu Ser Ile Val Met Pro Val Gly Gly Gln Ser Ser Phe Tyr  
65                  70                  75                  80

Ser Asp Trp Tyr Ser Pro Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr  
                85                  90                  95

Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala  
            100                 105                 110

Asn Arg Ala Val Lys Pro Thr Gly Ser Ala Ala Ile Gly Leu Ser Met  
        115                 120                 125

Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe  
    130                 135                 140

Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met  
145                 150                 155                 160

Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys  
                165                 170                 175

Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn 
           180                 185                 190

Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu  
        195                 200                 205

Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn 
    210                 215                 220

Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu Lys  
225                 230                 235                 240

Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn 
                245                 250                 255

Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu  
            260                 265                 270

Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly 
        275                 280                 285


<210> 6
<211> 288
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium ESAT-6

<400> 6
atgacagagc agcagtggaa tttcgcgggt atcgaggccg cggcaagcgc aatccaggga  60

aatgtcacgt ccattcattc cctccttgac gaggggaagc agtccctgac caagctcgca  120  

gcggcctggg gcggtagcgg ttcggaggcg taccagggtg tccagcaaaa atgggacgcc  180

acggctaccg agctgaacaa cgcgctgcag aacctggcgc ggacgatcag cgaagccggt  240

caggcaatgg cttcgaccga aggcaacgtc actgggatgt tcgcatag               288


<210> 7
<211> 282
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium ESAT-6 (human 
      optimized)

<400> 7
accgagcagc agtggaactt cgccggcatc gaagctgccg ctagcgccat ccaaggcaac  60

gtgaccagca tccacagcct gctggacgag ggcaagcaga gcctgaccaa gctggctgct  120

gcttggggcg gatccggaag cgaagcctac cagggcgtgc agcagaagtg ggacgccaca  180

gccaccgagc tgaacaacgc cctgcagaac ctcgccagaa ccatcagcga ggccggacag  240

gctatggcca gcacagaggg caatgtgacc ggcatgttcg cc                     282



<210> 8
<211> 94
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT-6

<400> 8
Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala 
1               5                   10                  15

Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys 
            20                  25                  30

Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu 
        35                  40                  45

Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu 
    50                  55                  60

Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln 
65                  70                  75                  80

Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala
                85                  90                 


<210> 9
<211> 687
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1980c

<400> 9
gtgcgcatca agatcttcat gctggtcacg gctgtcgttt tgctctgttg ttcgggtgtg  60

gccacggccg cgcccaagac ctactgcgag gagttgaaag gcaccgatac cggccaggcg  120

tgccagattc aaatgtccga cccggcctac aacatcaaca tcagcctgcc cagttactac  180

cccgaccaga agtcgctgga aaattacatc gcccagacgc gcgacaagtt cctcagcgcg  240

gccacatcgt ccactccacg cgaagccccc tacgaattga atatcacctc ggccacatac  300

cagtccgcga taccgccgcg tggtacgcag gccgtggtgc tcaaggtcta ccagaacgcc  360

ggcggcacgc acccaacgac cacgtacaag gccttcgatt gggaccaggc ctatcgcaag  420

ccaatcacct atgacacgct gtggcaggct gacaccgatc cgctgccagt cgtcttcccc  480

attgtgcaag gtgaactgag caagcagacc ggacaacagg tatcgatagc gccgaatgcc  540

ggcttggacc cggtgaatta tcagaacttc gcagtcacga acgacggggt gattttcttc  600

ttcaacccgg gggagttgct gcccgaagca gccggcccaa cccaggtatt ggtcccacgt  660

tccgcgatcg actcgatgct ggcctag                                      687


<210> 10
<211> 690
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1980c (human 
      optimized)

<400> 10
atggtcagga tcaagatctt catgctcgtg accgccgtgg tgctcctgtg ttgttccggc  60

gtggctaccg ctgctcccaa gacctactgc gaggagctga agggaaccga caccggccag  120

gcctgccaga tccaaatgag cgaccccgcc tacaacatca acatctccct cccctcctac  180

taccccgatc agaagtccct cgagaactac atcgctcaga ccagggacaa gttcctgagc  240

gccgccacaa gcagcacacc cagagaggcc ccctacgagc tgaacatcac ctccgccacc  300

taccagtccg ctattcctcc cagaggcacc caggctgtgg tgctcaaggt ctaccaaaac  360

gctggcggaa cacaccccac caccacctac aaggccttcg actgggacca ggcctacagg  420

aagcccatca catacgacac cctgtggcag gctgataccg atcccctgcc cgtggtgttc  480

cccatcgtgc agggcgagct ctccaagcag accggccagc aagtgagcat cgcccccaat  540

gctggactgg accccgtgaa ctaccagaac ttcgccgtca ccaacgacgg cgtgatcttc  600

ttcttcaatc ccggcgaact gctgcctgaa gctgctggcc ccacccaagt gctggtgcct  660

agaagcgcca tcgactccat gctggcctga                                   690


<210> 11
<211> 228
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv1980c 

<400> 11
Val Arg Ile Lys Ile Phe Met Leu Val Thr Ala Val Val Leu Leu Cys
1               5                   10                  15

Cys Ser Gly Val Ala Thr Ala Ala Pro Lys Thr Tyr Cys Glu Glu Leu 
            20                  25                  30

Lys Gly Thr Asp Thr Gly Gln Ala Cys Gln Ile Gln Met Ser Asp Pro 
        35                  40                  45

Ala Tyr Asn Ile Asn Ile Ser Leu Pro Ser Tyr Tyr Pro Asp Gln Lys 
    50                  55                  60

Ser Leu Glu Asn Tyr Ile Ala Gln Thr Arg Asp Lys Phe Leu Ser Ala 
65                  70                  75                  80

Ala Thr Ser Ser Thr Pro Arg Glu Ala Pro Tyr Glu Leu Asn Ile Thr 
                85                  90                  95

Ser Ala Thr Tyr Gln Ser Ala Ile Pro Pro Arg Gly Thr Gln Ala Val 
            100                 105                 110

Val Leu Lys Val Tyr Gln Asn Ala Gly Gly Thr His Pro Thr Thr Thr 
        115                 120                 125

Tyr Lys Ala Phe Asp Trp Asp Gln Ala Tyr Arg Lys Pro Ile Thr Tyr 
    130                 135                 140

Asp Thr Leu Trp Gln Ala Asp Thr Asp Pro Leu Pro Val Val Phe Pro 
145                 150                 155                 160

Ile Val Gln Gly Glu Leu Ser Lys Gln Thr Gly Gln Gln Val Ser Ile 
                165                 170                 175

Ala Pro Asn Ala Gly Leu Asp Pro Val Asn Tyr Gln Asn Phe Ala Val 
            180                 185                 190

Thr Asn Asp Gly Val Ile Phe Phe Phe Asn Pro Gly Glu Leu Leu Pro 
        195                 200                 205

Glu Ala Ala Gly Pro Thr Gln Val Leu Val Pro Arg Ser Ala Ile Asp 
    210                 215                 220

Ser Met Leu Ala 
225


<210> 12
<211> 1176
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1039c

<400> 12
atggatttcg gagctttacc ccctgagatc aactccgcac gcatgtacgc cggcgcgggt  60

gcaggaccga tgatggccgc cggggccgca tggaacggcc tggccgccga gttgggtacg  120

acggccgcgt cgtatgagtc ggtgatcacc cggctgacca ccgagtcgtg gatgggtccg  180

gcctcgatgg cgatggtcgc cgcagcccag ccctatctgg cttggttgac ctacaccgcc  240

gaagccgctg cgcatgccgg ctcgcaggcc atggcgtcgg cggccgccta cgaggcggcc  300

tatgcgatga cagtgccgcc ggaggtggtc gcggccaacc gggcgctgct ggcggccctg  360

gtcgcgacga acgtcctggg gatcaacaca ccggcaatca tggcgaccga agccctctat  420

gccgagatgt gggctcagga cgctctggct atgtacggct acgcggccgc ttcgggagcc  480

gccgggatgc tgcaaccgtt aagcccgccg tcgcagacca ccaacccggg cgggctggcc  540

gcccagtccg ccgcggtcgg ctcggctgcc gccaccgccg ccgtcaacca ggtgagcgta  600

gcggacctga tcagtagcct gcccaacgcg gtgagtgggc tcgcctcccc agtcacatcg  660

gttctcgact cgacggggct gagcggaatc attgccgaca tcgacgccct gctcgcgacc  720

ccgttcgtgg caaacatcat caacagcgca gtcaacaccg ccgcttggta tgtcaacgcc  780

gccatcccca ccgcgatatt cctagcaaat gccctgaaca gtggggcgcc ggtagcgatc  840

gccgaaggcg ccatcgaggc tgccgagggt gccgccagtg cggccgccgc ggggttggcg  900

gactcggtga cgccagcggg gctcggcgca agtttaggcg aggccaccct ggtcggccgg  960

ctgtcagtgc cggcggcctg gtctacggcc gcaccggcga caaccgccgg cgccacagcg 1020

ctcgaaggca gcggctggac cgtcgccgcc gaagaagccg gcccagttac cgggatgatg 1080

ccgggaatgg cctcggccgc caagggcacc ggtgcctatg ccgggccgcg gtacggattc 1140

aagcccactg tcatgcccaa acaggtcgtc gtgtga                           1176


<210> 13
<211> 1176
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1039c (human 
      optimized)

<400> 13
atggattttg gcgccctgcc tcccgagatc aacagcgcta ggatgtatgc tggcgctgga  60

gccggaccta tgatggccgc tggagccgcc tggaatggac tggctgccga actgggcaca  120

acagccgctt cctacgagtc cgtgatcacc agactcacca cagagtcctg gatgggacct  180

gccagcatgg ctatggtcgc cgctgctcaa ccctacctgg cctggctgac ctatacagct  240

gaagccgctg ctcacgccgg aagccaagct atggctagcg ccgccgctta tgaggccgct  300

tatgccatga ccgtgcctcc cgaggtcgtg gctgccaaca gagctctcct ggccgccctc  360

gtggctacca acgtgctggg aatcaacacc cccgctatta tggccaccga ggctctgtac  420

gctgagatgt gggcccagga tgccctcgcc atgtacggat acgccgctgc ttccggagct  480

gctggaatgc tgcagcccct gtccccccct tcccagacca ccaaccccgg aggactggct  540

gctcaaagcg ctgctgtggg atccgctgct gctaccgctg ccgtcaatca ggtcagcgtc  600

gccgacctca tctccagcct gcctaacgct gtgagcggac tggcctcccc tgtcacatcc  660

gtgctcgata gcaccggcct gtccggcatc atcgccgaca ttgatgctct cctcgccacc  720

ccctttgtcg ccaacatcat caattccgcc gtgaacaccg ctgcctggta cgtcaacgct  780

gccattccca ccgccatctt cctcgccaac gccctgaact ccggagctcc tgtcgccatc  840

gctgagggcg ctattgaggc tgctgaagga gccgctagcg ctgctgctgc tggactggct  900

gatagcgtca cccctgctgg actcggagct agcctgggag aagccaccct ggtcggcaga  960

ctgtccgtgc ctgctgcttg gagcaccgct gctcctgcta caaccgctgg agctaccgct 1020

ctggagggat ccggatggac agtggctgct gaggaagctg gacccgtgac cggaatgatg 1080

cctggcatgg ccagcgctgc taagggaacc ggcgcctatg ccggacccag atacggattc 1140

aagcccaccg tcatgcccaa gcaggtcgtc gtctaa                           1176


<210> 14
<211> 401
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv1039c 

<400> 14
Met Asp Phe Gly Ala Leu Pro Pro Glu Ile Asn Ser Ala Arg Met Tyr 
1               5                   10                  15

Ala Gly Ala Gly Ala Gly Pro Met Met Ala Ala Gly Ala Ala Trp Asn 
            20                  25                  30

Gly Leu Ala Ala Glu Leu Gly Thr Thr Ala Ala Ser Tyr Glu Ser Val 
        35                  40                  45

Ile Thr Arg Leu Thr Thr Glu Ser Trp Met Gly Pro Ala Ser Met Ala 
    50                  55                  60

Met Val Ala Ala Ala Gln Pro Tyr Leu Ala Trp Leu Thr Tyr Thr Ala 
65                  70                  75                  80

Glu Ala Ala Ala His Ala Gly Ser Gln Ala Met Ala Ser Ala Ala Ala 
                85                  90                  95

Tyr Glu Ala Ala Tyr Ala Met Thr Val Pro Pro Glu Val Val Ala Ala 
            100                 105                 110

Asn Arg Ala Leu Leu Ala Ala Leu Val Ala Thr Asn Val Leu Gly Ile 
        115                 120                 125

Asn Thr Pro Ala Ile Met Ala Thr Glu Ala Leu Tyr Ala Glu Met Trp 
    130                 135                 140

Ala Gln Asp Ala Leu Ala Met Tyr Gly Tyr Ala Ala Ala Ser Gly Ala 
145                 150                 155                 160

Ala Gly Met Leu Gln Pro Leu Ser Pro Pro Ser Gln Thr Thr Asn Pro 
                165                 170                 175

Gly Gly Leu Ala Ala Gln Ser Ala Ala Val Gly Ser Ala Ala Ala Thr 
            180                 185                 190

Ala Ala Val Asn Gln Val Ser Val Ala Asp Leu Ile Ser Ser Leu Pro 
        195                 200                 205

Asn Ala Val Ser Gly Leu Ala Ser Pro Val Thr Ser Val Leu Asp Ser 
    210                 215                 220

Thr Gly Leu Ser Gly Ile Ile Ala Asp Ile Asp Ala Leu Leu Ala Thr 
225                 230                 235                 240

Pro Phe Val Ala Asn Ile Ile Asn Ser Ala Val Asn Thr Ala Ala Trp 
                245                 250                 255

Tyr Val Asn Ala Ala Ile Pro Thr Ala Ile Phe Leu Ala Asn Ala Leu 
            260                 265                 270

Asn Ser Gly Ala Pro Val Ala Ile Ala Glu Gly Ala Ile Glu Ala Ala 
        275                 280                 285

Glu Gly Ala Ala Ser Ala Ala Ala Ala Gly Leu Ala Asp Ser Val Thr 
    290                 295                 300

Pro Ala Gly Leu Gly Ala Ser Leu Gly Glu Ala Thr Leu Val Gly Arg 
305                 310                 315                 320

Leu Ser Val Pro Ala Ala Trp Ser Thr Ala Ala Pro Ala Thr Thr Ala 
                325                 330                 335

Gly Ala Thr Ala Leu Glu Gly Ser Gly Trp Thr Val Ala Ala Glu Glu 
            340                 345                 350

Ala Gly Pro Val Thr Gly Met Met Pro Gly Met Ala Ser Ala Ala Lys 
        355                 360                 365

Gly Thr Gly Ala Tyr Ala Gly Pro Arg Tyr Gly Phe Lys Pro Thr Val 
    370                 375                 380

Met Pro Lys Gln Val Val Val 
385                 400


<210> 15
<211> 1143
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3136

<400> 15
atggatttcg cactgttacc accggaagtc aactccgccc ggatgtacac cggccctggg  60

gcaggatcgc tgttggctgc cgcgggcggc tgggattcgc tggccgccga gttggccacc  120

acagccgagg catatggatc ggtgctgtcc ggactggccg ccttgcattg gcgtggaccg  180

gcagcggaat cgatggcggt gacggccgct ccctatatcg gttggctgta cacgaccgcc  240

gaaaagacac agcaaacagc gatccaagcc agggcggcag cgctggcctt cgagcaagca  300

tacgcaatga ccctgccgcc accggtggta gcggccaacc ggatacagct gctagcactg  360

atcgcgacga acttcttcgg ccagaacact gcggcgatcg cggccaccga ggcacagtac  420

gccgagatgt gggcccagga cgccgccgcg atgtacggtt acgccaccgc ctcagcggct  480

gcggccctgc tgacaccgtt ctccccgccg cggcagacca ccaacccggc cggcctgacc  540

gctcaggccg ccgcggtcag ccaggccacc gacccactgt cgctgctgat tgagacggtg  600

acccaagcgc tgcaagcgct gacgattccg agcttcatcc ctgaggactt caccttcctt  660

gacgccatat tcgctggata tgccacggta ggtgtgacgc aggatgtcga gtcctttgtt  720

gccgggacca tcggggccga gagcaaccta ggccttttga acgtcggcga cgagaatccc  780

gcggaggtga caccgggcga ctttgggatc ggcgagttgg tttccgcgac cagtcccggc  840

ggtggggtgt ctgcgtcggg tgccggcggt gcggcgagcg tcggcaacac ggtgctcgcg  900

agtgtcggcc gggcaaactc gattgggcaa ctatcggtcc caccgagctg ggccgcgccc  960

tcgacgcgcc ctgtctcggc attgtcgccc gccggcctga ccacactccc ggggaccgac 1020

gtggccgagc acgggatgcc aggtgtaccg ggggtgccag tggcagcagg gcgagcctcc 1080

ggcgtcctac ctcgatacgg ggttcggctc acggtgatgg cccacccacc cgcggcaggg 1140

taa                                                               1143


<210> 16
<211> 1146
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3136 (E. coli 
      optimized) 

<400> 16
atggattttg cgctgctgcc gccggaagtg aacagcgcgc gcatgtatac cggcccgggc  60 

gcgggcagcc tgctggcggc ggcgggcggc tgggatagcc tggcggcgga actggcgacc  120

accgcggaag cgtatggcag cgtgctgagc ggcctggcgg cgctgcattg gcgcggcccg  180

gcggcggaaa gcatggcggt gaccgcggcg ccgtatattg gctggctgta taccaccgcg  240

gaaaaaaccc agcagaccgc gattcaggcg cgcgcggcgg cgctggcgtt tgaacaggcg  300

tatgcgatga ccctgccgcc gccggtggtg gcggcgaacc gcattcagct gctggcgctg  360

attgcgacca acttttttgg ccagaacacc gcggcgattg cggcgaccga agcgcagtat  420

gcggaaatgt gggcgcagga tgcggcggcg atgtatggct atgcgaccgc gagcgcggcg  480

gcggcgctgc tgaccccgtt tagcccgccg cgccagacca ccaacccggc gggcctgacc  540

gcgcaggcgg cggcggtgag ccaggcgacc gatccgctga gcctgctgat tgaaaccgtg  600

acccaggcgc tgcaggcgct gaccattccg agctttattc cggaagattt tacctttctg  660

gatgcgattt ttgcgggcta tgcgaccgtg ggcgtgaccc aggatgtgga aagctttgtg  720

gcgggcacca ttggcgcgga aagcaacctg ggcctgctga acgtgggcga tgaaaacccg  780

gcggaagtga ccccgggcga ttttggcatt ggcgaactgg tgagcgcgac cagcccgggc  840

ggcggcgtga gcgcgagcgg cgcgggcggc gcggcgagcg tgggcaacac cgtgctggcg  900

agcgtgggcc gcgcgaacag cattggccag ctgagcgtgc cgccgagctg ggcggcgccg  960

agcacccgcc cggtgagcgc gctgagcccg gcgggcctga ccaccctgcc gggcaccgat 1020

gtggcggaac atggcatgcc gggcgtgccg ggcgtgccgg tggcggcggg ccgcgcgagc 1080

ggcgtgctgc cgcgctatgg cgtgcgcctg accgtgatgg cgcatccgcc ggcggcgggc 1140

gaattt                                                            1146


<210> 17
<211> 1143
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3136 (human 
      optimized)

<400> 17
atggatttcg ctctgctccc ccccgaggtg aatagcgcta ggatgtacac aggacccgga  60

gctggaagcc tcctggctgc tgctggagga tgggactccc tggctgccga gctcgctaca  120

accgctgagg cttacggaag cgtgctctcc ggcctggctg ctctccattg gagaggccct  180

gctgccgagt ccatggctgt cacagccgct ccctacattg gatggctgta caccaccgcc  240

gagaagaccc agcaaaccgc tattcaggcc agagctgccg ccctggcctt cgaacaggcc  300

tacgctatga cactcccccc ccctgtcgtg gctgccaata ggatccagct cctggccctc  360

atcgccacca acttcttcgg ccaaaacacc gctgccatcg ctgccaccga agcccagtac  420

gccgaaatgt gggcccagga tgccgctgct atgtacggct atgccacagc tagcgctgcc  480

gctgctctgc tcacaccctt cagccccccc aggcaaacaa ccaaccctgc cggactgaca  540

gcccaagctg ctgccgtcag ccaagctacc gaccccctga gcctcctgat cgaaaccgtg  600

acacaggccc tgcaggccct gaccattccc agctttatcc ccgaggactt cacctttctg  660

gacgctatct tcgctggcta cgccaccgtg ggcgtgacac aagacgtcga gtccttcgtc  720

gccggcacaa tcggagccga gtccaacctc ggactcctca acgtcggcga cgaaaatccc  780

gccgaagtga cacctggaga cttcggcatt ggagaactcg tcagcgccac atcccctggc  840

ggaggagtga gcgcttccgg agctggagga gctgcttccg tgggcaatac cgtgctggcc  900

agcgtgggaa gggccaactc cattggccag ctcagcgtcc ccccttcctg ggctgcccct  960

tccacaaggc ctgtgtccgc tctcagccct gctggactga ccacactccc tggcacagac 1020

gtggctgagc atggcatgcc cggagtgcct ggagtccctg tggctgctgg cagagcttcc 1080

ggagtcctcc ctaggtatgg cgtgaggctg acagtgatgg ctcatccccc cgctgccgga 1140

taa                                                               1143


<210> 18
<211> 380
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv3136

<400> 18
Met Asp Phe Ala Leu Leu Pro Pro Glu Val Asn Ser Ala Arg Met Tyr 
1               5                   10                  15

Thr Gly Pro Gly Ala Gly Ser Leu Leu Ala Ala Ala Gly Gly Trp Asp 
            20                  25                  30

Ser Leu Ala Ala Glu Leu Ala Thr Thr Ala Glu Ala Tyr Gly Ser Val 
        35                  40                  45

Leu Ser Gly Leu Ala Ala Leu His Trp Arg Gly Pro Ala Ala Glu Ser 
    50                  55                  60

Met Ala Val Thr Ala Ala Pro Tyr Ile Gly Trp Leu Tyr Thr Thr Ala 
65                  70                  75                  80

Glu Lys Thr Gln Gln Thr Ala Ile Gln Ala Arg Ala Ala Ala Leu Ala 
                85                  90                  95

Phe Glu Gln Ala Tyr Ala Met Thr Leu Pro Pro Pro Val Val Ala Ala 
            100                 105                 110

Asn Arg Ile Gln Leu Leu Ala Leu Ile Ala Thr Asn Phe Phe Gly Gln 
        115                 120                 125

Asn Thr Ala Ala Ile Ala Ala Thr Glu Ala Gln Tyr Ala Glu Met Trp 
    130                 135                 140

Ala Gln Asp Ala Ala Ala Met Tyr Gly Tyr Ala Thr Ala Ser Ala Ala 
145                 150                 155                 160

Ala Ala Leu Leu Thr Pro Phe Ser Pro Pro Arg Gln Thr Thr Asn Pro 
                165                 170                 175

Ala Gly Leu Thr Ala Gln Ala Ala Ala Val Ser Gln Ala Thr Asp Pro 
            180                 185                 190

Leu Ser Leu Leu Ile Glu Thr Val Thr Gln Ala Leu Gln Ala Leu Thr 
        195                 200                 205

Ile Pro Ser Phe Ile Pro Glu Asp Phe Thr Phe Leu Asp Ala Ile Phe 
    210                 215                 220

Ala Gly Tyr Ala Thr Val Gly Val Thr Gln Asp Val Glu Ser Phe Val 
225                 230                 235                 240

Ala Gly Thr Ile Gly Ala Glu Ser Asn Leu Gly Leu Leu Asn Val Gly 
                245                 250                 255

Asp Glu Asn Pro Ala Glu Val Thr Pro Gly Asp Phe Gly Ile Gly Glu 
            260                 265                 270

Leu Val Ser Ala Thr Ser Pro Gly Gly Gly Val Ser Ala Ser Gly Ala 
        275                 280                 285

Gly Gly Ala Ala Ser Val Gly Asn Thr Val Leu Ala Ser Val Gly Arg 
    290                 295                 300

Ala Asn Ser Ile Gly Gln Leu Ser Val Pro Pro Ser Trp Ala Ala Pro 
305                 310                 315                 320

Ser Thr Arg Pro Val Ser Ala Leu Ser Pro Ala Gly Leu Thr Thr Leu 
                325                 330                 335

Pro Gly Thr Asp Val Ala Glu His Gly Met Pro Gly Val Pro Gly Val 
            340                 345                 350

Pro Val Ala Ala Gly Arg Ala Ser Gly Val Leu Pro Arg Tyr Gly Val 
        355                 360                 365

Arg Leu Thr Val Met Ala His Pro Pro Ala Ala Gly
    370                 375                 380


<210> 19
<211> 312
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3615c

<400> 19
atgacggaaa acttgaccgt ccagcccgag cgtctcggtg tactggcgtc gcaccatgac  60

aacgcggcgg tcgatgcctc ctcgggcgtc gaagctgccg ctggcctagg cgaatctgtg  120

gcgatcactc acggtccgta ctgctcacag ttcaacgaca cgttaaatgt gtacttgact  180

gcccacaatg ccctgggctc gtccttgcat acggccggtg tcgatctcgc caaaagtctt  240

cgaattgcgg cgaagatata tagcgaggcc gacgaagcgt ggcgcaaggc tatcgacggg  300

ttgtttacct ga                                                      312


<210> 20
<211> 312
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3615c (human 
      optimized)

<400> 20
atgaccgaga acctgaccgt gcagcctgag aggctgggag tgctggccag ccaccacgac  60

aacgctgccg tggacgcttc cagcggagtg gaggctgctg ctggactggg agagagcgtg  120

gccatcaccc acggacccta ctgcagccag ttcaacgaca ccctgaacgt gtacctgaca  180

gcccacaacg ccctgggaag cagcctgcat acagccggcg tggacctggc taagtccctg  240

aggatcgccg ccaagatcta cagcgaggcc gacgaggcct ggaggaaagc catcgacggc  300

ctgttcacct aa                                                      312


<210> 21
<211> 103
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv3615c 

<400> 21
Met Thr Glu Asn Leu Thr Val Gln Pro Glu Arg Leu Gly Val Leu Ala 
1               5                   10                  15

Ser His His Asp Asn Ala Ala Val Asp Ala Ser Ser Gly Val Glu Ala 
            20                  25                  30

Ala Ala Gly Leu Gly Glu Ser Val Ala Ile Thr His Gly Pro Tyr Cys 
        35                  40                  45

Ser Gln Phe Asn Asp Thr Leu Asn Val Tyr Leu Thr Ala His Asn Ala 
    50                  55                  60

Leu Gly Ser Ser Leu His Thr Ala Gly Val Asp Leu Ala Lys Ser Leu 
65                  70                  75                  80

Arg Ile Ala Ala Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys 
                85                  90                  95

Ala Ile Asp Gly Leu Phe Thr 
            100


<210> 22
<211> 633
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1733c

<400> 22
atgatcgcca caacccgcga tcgtgaagga gccaccatga tcacgtttag gctgcgcttg  60

ccgtgccgga cgatactgcg ggtgttcagc cgcaatccgc tggtgcgtgg gacggatcga  120

ctcgaggcgg tcgtcatgct gctggccgtc acggtctcgc tgctgactat cccgttcgcc  180

gccgcggccg gcaccgcagt ccaggattcc cgcagccacg tctatgccca ccaggcccag  240

acccgccatc ccgcaaccgc gaccgtgatc gatcacgagg gggtgatcga cagcaacacg  300

accgccacgt cagcgccgcc gcgcacgaag atcaccgtgc ctgcccgatg ggtcgtgaac  360

ggaatagaac gcagcggtga ggtcaacgcg aagccgggaa ccaaatccgg tgaccgcgtc  420

ggcatttggg tcgacagtgc cggtcagctg gtcgatgaac cagctccgcc ggcccgtgcc  480

attgcggatg cggccctggc cgccttggga ctctggttga gcgtcgccgc ggttgcgggc  540

gccctgctgg cgctcactcg ggcgattctg atccgcgttc gcaacgccag ttggcaacac  600

gacatcgaca gcctgttctg cacgcagcgg tga                               633


<210> 23
<211> 630
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1733c (E. coli 
      optimized)

<400> 23
atgattgcga ctacccgtga tcgtgagggc gcgaccatga tcacgttccg tctgcgtctg  60

ccgtgtcgca ccattttgcg cgtgttttcg cgtaacccgc tggtccgcgg taccgaccgt  120

ctggaggccg ttgtcatgct gctggcggtt accgtgagcc tgctgacgat cccattcgca  180

gcggcagctg gcacggccgt ccaagacagc cgtagccatg tgtatgctca ccaggctcaa  240

acccgtcacc cggctactgc cactgttatc gatcacgaag gcgtgattga ctccaatacc  300

acggcaacct ccgcaccgcc tcgcaccaag attacggttc ctgcgcgttg ggtggtgaat  360

ggtattgaac gcagcggcga agttaatgcc aaaccgggta ccaaaagcgg tgaccgtgtg  420

ggcatctggg tcgatagcgc cggtcagctg gtcgacgagc cggcaccgcc agcgcgtgcg  480

atcgccgatg cggcgctggc tgccctgggt ctgtggctga gcgtggcagc ggtcgccggt  540

gcgttgctgg cgctgacgcg cgcaattctg atccgcgttc gcaatgcgag ctggcagcac  600

gatattgata gcctgttttg cacccaacgt                                   630


<210> 24
<211> 210
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv1733c 

<400> 24
Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe 
1               5                   10                  15

Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn 
            20                  25                  30

Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Val Val Met Leu Leu 
        35                  40                  45

Ala Val Thr Val Ser Leu Leu Thr Ile Pro Phe Ala Ala Ala Ala Gly 
    50                  55                  60

Thr Ala Val Gln Asp Ser Arg Ser His Val Tyr Ala His Gln Ala Gln 
65                  70                  75                  80

Thr Arg His Pro Ala Thr Ala Thr Val Ile Asp His Glu Gly Val Ile 
                85                  90                  95

Asp Ser Asn Thr Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr 
            100                 105                 110

Val Pro Ala Arg Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val 
        115                 120                 125

Asn Ala Lys Pro Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val 
    130                 135                 140

Asp Ser Ala Gly Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala 
145                 150                 155                 160

Ile Ala Asp Ala Ala Leu Ala Ala Leu Gly Leu Trp Leu Ser Val Ala 
                165                 170                 175

Ala Val Ala Gly Ala Leu Leu Ala Leu Thr Arg Ala Ile Leu Ile Arg 
            180                 185                 190

Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys Thr 
        195                 200                 205

Gln Arg 
    210


<210> 25
<211> 454
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1733c 
      (transmembrane deleted; E. coli optimized) 

<400> 25
atgattgcga ctacccgtga tcgtgagggc gcgaccatga tcacgttccg tctgcgtctg  60

ccgtgtcgca ccattttgcg cgtgttttcg cgtaacccgc tggtccgcgg taccgaccgt  120

ctggaggccc ccggggtcca agacagccgt agccatgtgt atgctcacca ggctcaaacc  180

cgtcacccgg ctactgccac tgttatcgat cacgaaggcg tgattgactc caataccacg  240

gcaacctccg caccgcctcg caccaagatt acggttcctg cgcgttgggt ggtgaatggt  300

attgaacgca gcggcgaagt taatgccaaa ccgggtacca aaagcggtga ccgtgtgggc  360

atctgggtcg atagcgccgg tcagctggtc gacgagccgg caccgccagc gcgtgcgatc  420

gccgattcta gacgcgcaat tctgatccgc gttcgcaatg cgagctggca gcacgatatt  480

gatagcctgt tttgcaccca acgt                                         454


<210> 26
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1733c 
      (transmembrane deleted; human optimized)

<400> 26
atcgccacca ccagggacag ggaaggcgct accatgatca ccttcaggct gaggctcccc  60

tgcaggacca tcctgagggt gttcagcagg aaccccctgg tgaggggcac cgacagactg  120

gaagccgtgc aggacagcag gagccacgtg tatgcccacc aggctcagac caggcaccct  180

gctaccgcca ccgtgatcga ccacgagggc gtgatcgact ccaacaccac cgccaccagc  240

gctcctccca gaaccaagat cacagtgccc gccaggtggg tggtgaacgg catcgagagg  300

agcggcgagg tgaacgccaa gcctggaacc aagagcggcg acagggtggg catttgggtc  360

gatagcgccg gccagctggt ggatgaacct gctccccctg ccagagccat cgccgatagg  420

gccatcctga tcagggtgag gaacgccagc tggcagcacg acatcgacag cctgttctgc  480

acccaaagg                                                          489


<210> 27
<211> 168
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv1733c (both transmembrane 
      regions deleted; E. coli optimized)

<400> 27
Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe 
1               5                   10                  15

Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn 
            20                  25                  30

Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Pro Gly Val Gln Asp 
        35                  40                  45

Ser Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro Ala 
    50                  55                  60

Thr Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr Thr 
65                  70                  75                  80

Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg Trp 
                85                  90                  95

Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro Gly 
            100                 105                 110

Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly Gln 
        115                 120                 125

Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Ser Arg 
    130                 135                 140

Arg Ala Ile Leu Ile Arg Val Arg Asn Ala Ser Trp Gln His Asp Ile 
145                 150                 155                 160

Asp Ser Leu Phe Cys Thr Gln Arg
                165  


<210> 28
<211> 163
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv1733c (both transmembrane 
      regions deleted; human optimized)

<400> 28
Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe Arg 
1               5                   10                  15

Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn Pro 
            20                  25                  30

Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Val Gln Asp Ser Arg Ser 
        35                  40                  45

His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro Ala Thr Ala Thr 
    50                  55                  60

Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr Thr Ala Thr Ser 
65                  70                  75                  80

Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg Trp Val Val Asn 
                85                  90                  95

Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro Gly Thr Lys Ser 
            100                 105                 110

Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly Gln Leu Val Asp 
        115                 120                 125

Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Arg Ala Ile Leu Ile 
    130                 135                 140

Arg Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys 
145                 150                 155                 160

Thr Gln Arg 


<210> 29
<211> 432
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2626c

<400> 29
atgaccaccg cacgcgacat catgaacgca ggtgtgacct gtgttggcga acacgagacg  60 

ctaaccgctg ccgctcaata catgcgtgag cacgacatcg gcgcgttgcc gatctgcggg  120

gacgacgacc ggctgcacgg catgctcacc gaccgcgaca ttgtgatcaa aggcctggct  180

gcgggcctag acccgaatac cgccacggct ggcgagttgg cccgggacag catctactac  240

gtcgatgcga acgcaagcat ccaggagatg ctcaacgtca tggaagaaca tcaggtccgc  300

cgtgttccgg tcatctcaga gcaccgcttg gtcggaatcg tcaccgaagc cgacatcgcc  360

cgacacctgc ccgagcacgc cattgtgcag ttcgtcaagg caatctgctc gcccatggcc  420

ctcgccagct ag                                                      432


<210> 30
<211> 428
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2626c (E. coli 
      optimized)

<400> 30
atgaccacgg cgcgtgatat catgaatgcg ggtgtcacct gtgttggcga gcacgaaacg  60

ttgaccgcag cagcacagta catgcgcgaa catgatatcg gcgcattgcc gatttgcggc  120

gacgatgatc gtctgcacgg tatgctgacc gaccgcgata tcgttatcaa gggtctggcc  180

gcaggcttgg acccgaacac cgcgaccgcc ggtgaactgg cacgtgacag catctattac  240

gtcgacgcga acgccagcat tcaagagatg ctgaacgtga tggaagagca tcaggtgcgt  300

cgtgtcccgg ttatcagcga acatcgtctg gttggtatcg ttaccgaagc cgacatcgca  360

cgtcacctgc cggagcacgc gattgttcag ttcgtgaaag cgatttgcag cccgatggcg  420

ttggcgtc                                                           428


<210> 31
<211> 426
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2626c (human 
      optimized)

<400> 31
acaacagcca gggacatcat gaacgccggc gtgacctgcg tgggagagca tgaaaccctc  60

accgccgccg cccaatacat gagggagcac gacatcggcg ccctgcccat ctgtggagac  120

gacgacaggc tgcacggcat gctgaccgac agggacatcg tgatcaaggg cctggctgcc  180

ggcctcgatc ctaacaccgc tacagccggc gagctggcca gagacagcat ctactacgtg  240

gacgccaacg ccagcatcca ggagatgctc aacgtgatgg aggagcacca ggtgagaagg  300

gtgcctgtga tcagcgagca caggctggtg ggcatcgtga ccgaggccga tatcgctagg  360

cacctgcccg agcacgccat cgtgcagttc gtgaaggcca tctgcagccc catggctctg  420

gccagc                                                             426


<210> 32
<211> 143
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv2626c 

<400> 32
Met Thr Thr Ala Arg Asp Ile Met Asn Ala Gly Val Thr Cys Val Gly 
1               5                   10                  15

Glu His Glu Thr Leu Thr Ala Ala Ala Gln Tyr Met Arg Glu His Asp 
            20                  25                  30

Ile Gly Ala Leu Pro Ile Cys Gly Asp Asp Asp Arg Leu His Gly Met 
        35                  40                  45

Leu Thr Asp Arg Asp Ile Val Ile Lys Gly Leu Ala Ala Gly Leu Asp 
    50                  55                  60

Pro Asn Thr Ala Thr Ala Gly Glu Leu Ala Arg Asp Ser Ile Tyr Tyr 
65                  70                  75                  80

Val Asp Ala Asn Ala Ser Ile Gln Glu Met Leu Asn Val Met Glu Glu 
                85                  90                  95

His Gln Val Arg Arg Val Pro Val Ile Ser Glu His Arg Leu Val Gly 
            100                 105                 110

Ile Val Thr Glu Ala Asp Ile Ala Arg His Leu Pro Glu His Ala Ile 
        115                 120                 125

Val Gln Phe Val Lys Ala Ile Cys Ser Pro Met Ala Leu Ala Ser 
    130                 135                 140


<210> 33
<211> 300
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3407

<400> 33
atgcgtgcta ccgttgggct tgtggaggca atcggaatcc gagaactaag acagcacgca  60

tcgcgatacc tcgcccgggt tgaagccggc gaggaacttg gcgtcaccaa caaaggaaga  120

cttgtggccc gactcatccc ggtgcaggcc gcggagcgtt ctcgcgaagc cctgattgaa  180

tcaggtgtcc tgattccggc tcgtcgtcca caaaaccttc tcgacgtcac cgccgaaccg  240

gcgcgcggcc gcaagcgcac cctgtccgat gttctcaacg aaatgcgcga cgagcagtga  300


<210> 34
<211> 297
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3407 (E. coli 
      optimized)

<400> 34
atgcgtgcga ctgtgggtct ggttgaggcg attggcattc gcgagctgcg ccaacatgcc  60

agccgttact tggctcgtgt cgaggcgggt gaagaactgg gcgtgacgaa taagggtcgt  120

ctggtcgccc gtctgattcc ggttcaggca gctgagcgtt ctcgcgaggc gctgattgaa  180

tccggcgtcc tgatcccggc tcgccgtccg caaaacctgc tggacgtgac ggcggagcca  240

gctcgtggtc gcaaacgcac gctgtctgat gtcctgaacg aaatgcgcga cgagcag     297


<210> 35
<211> 297
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3407 (human 
      optimized)

<400> 35
atgagggcga ccgtcgggct ggtggaggcg ataggtatcc gggagttgcg acagcacgca  60

tcacgatatc tggcacgggt ggaagctggg gaggaactgg gcgtgaccaa caaggggcgg  120

ctggtcgcga ggctgatccc cgtgcaggcc gccgagcggt cccgcgaagc cctcatcgag  180

tctggggtgc tcattccagc acgcaggccg caaaatctcc tggacgtcac tgcggagccc  240

gccagaggca gaaagaggac gctgagtgac gtgctgaacg agatgaggga cgaacag     297


<210> 36
<211> 99
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv3407

<400> 36
Met Arg Ala Thr Val Gly Leu Val Glu Ala Ile Gly Ile Arg Glu Leu 
1               5                   10                  15

Arg Gln His Ala Ser Arg Tyr Leu Ala Arg Val Glu Ala Gly Glu Glu 
            20                  25                  30

Leu Gly Val Thr Asn Lys Gly Arg Leu Val Ala Arg Leu Ile Pro Val 
        35                  40                  45

Gln Ala Ala Glu Arg Ser Arg Glu Ala Leu Ile Glu Ser Gly Val Leu 
    50                  55                  60

Ile Pro Ala Arg Arg Pro Gln Asn Leu Leu Asp Val Thr Ala Glu Pro 
65                  70                  75                  80

Ala Arg Gly Arg Lys Arg Thr Leu Ser Asp Val Leu Asn Glu Met Arg 
                85                  90                  95

Asp Glu Gln 


<210> 37
<211> 363
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2628c

<400> 37
atgtccacgc aacgaccgag gcactccggt attcgggctg ttggccccta cgcatgggcc  60

ggccgatgtg gtcggatagg caggtggggg gtgcaccagg aggcgatgat gaatctagcg  120

atatggcacc cgcgcaaggt gcaatccgcc accatctatc aggtgaccga tcgctcgcac  180

gacgggcgca cagcacgggt gcctggtgac gagatcacta gcaccgtgtc cggttggttg  240

tcggagttgg gcacccaaag cccgttggcc gatgagcttg cgcgtgcggt gcggatcggc  300

gactggcccg ctgcgtacgc aatcggtgag cacctgtccg ttgagattgc cgttgcggtc  360

taa                                                                363


<210> 38
<211> 363
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2628c (human 
      optimized)

<400> 38
atgagcaccc agagacccag gcacagcggc attagggccg tgggacctta tgcttgggcc  60

ggcagatgcg gaaggatcgg cagatggggc gtgcaccaag aggccatgat gaacctggcc  120

atctggcacc ccaggaaggt gcagagcgcc accatctacc aggtgaccga caggagccat  180

gacggaagga ccgccagagt gcccggcgat gagatcacca gcaccgtgag cggctggctg  240

agcgaactgg gcacccaatc ccccctggct gatgaactgg ccagggctgt gaggatcggc  300

gattggcctg ccgcctatgc catcggcgag catctgagcg tggagatcgc cgtggccgtg  360

taa                                                                363


<210> 39
<211> 120
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv2628c 

<400> 39
Met Ser Thr Gln Arg Pro Arg His Ser Gly Ile Arg Ala Val Gly Pro 
1               5                   10                  15

Tyr Ala Trp Ala Gly Arg Cys Gly Arg Ile Gly Arg Trp Gly Val His 
            20                  25                  30

Gln Glu Ala Met Met Asn Leu Ala Ile Trp His Pro Arg Lys Val Gln 
        35                  40                  45

Ser Ala Thr Ile Tyr Gln Val Thr Asp Arg Ser His Asp Gly Arg Thr 
    50                  55                  60

Ala Arg Val Pro Gly Asp Glu Ile Thr Ser Thr Val Ser Gly Trp Leu 
65                  70                  75                  80

Ser Glu Leu Gly Thr Gln Ser Pro Leu Ala Asp Glu Leu Ala Arg Ala 
                85                  90                  95

Val Arg Ile Gly Asp Trp Pro Ala Ala Tyr Ala Ile Gly Glu His Leu 
            100                 105                 110

Ser Val Glu Ile Ala Val Ala Val 
        115                 120

<210> 40
<211> 1089
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1009

<400> 40
atgttgcgcc tggtagtcgg tgcgctgctg ctggtgttgg cgttcgccgg tggctatgcg  60

gtcgccgcat gcaaaacggt gacgttgacc gtcgacggaa ccgcgatgcg ggtgaccacg  120

atgaaatcgc gggtgatcga catcgtcgaa gagaacgggt tctcagtcga cgaccgcgac  180

gacctgtatc ccgcggccgg cgtgcaggtc catgacgccg acaccatcgt gctgcggcgt  240

agccgtccgc tgcagatctc gctggatggt cacgacgcta agcaggtgtg gacgaccgcg  300

tcgacggtgg acgaggcgct ggcccaactc gcgatgaccg acacggcgcc ggccgcggct  360

tctcgcgcca gccgcgtccc gctgtccggg atggcgctac cggtcgtcag cgccaagacg  420

gtgcagctca acgacggcgg gttggtgcgc acggtgcact tgccggcccc caatgtcgcg  480

gggctgctga gtgcggccgg cgtgccgctg ttgcaaagcg accacgtggt gcccgccgcg  540

acggccccga tcgtcgaagg catgcagatc caggtgaccc gcaatcggat caagaaggtc  600

accgagcggc tgccgctgcc gccgaacgcg cgtcgtgtcg aggacccgga gatgaacatg  660

agccgggagg tcgtcgaaga cccgggggtt ccggggaccc aggatgtgac gttcgcggta  720

gctgaggtca acggcgtcga gaccggccgt ttgcccgtcg ccaacgtcgt ggtgaccccg  780

gcccacgaag ccgtggtgcg ggtgggcacc aagcccggta ccgaggtgcc cccggtgatc  840

gacggaagca tctgggacgc gatcgccggc tgtgaggccg gtggcaactg ggcgatcaac  900

accggcaacg ggtattacgg tggtgtgcag tttgaccagg gcacctggga ggccaacggc  960

gggctgcggt atgcaccccg cgctgacctc gccacccgcg aagagcagat cgccgttgcc  1020

gaggtgaccc gactgcgtca aggttggggc gcctggccgg tatgtgctgc acgagcgggt  1080

gcgcgctga                                                          1089


<210> 41
<211> 1023
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv1009 (signal 
      sequence deleted)

<400> 41
gcatgcaaaa cggtgacgtt gaccgtcgac ggaaccgcga tgcgggtgac cacgatgaaa  60

tcgcgggtga tcgacatcgt cgaagagaac gggttctcag tcgacgaccg cgacgacctg  120

tatcccgcgg ccggcgtgca ggtccatgac gccgacacca tcgtgctgcg gcgtagccgt  180

ccgctgcaga tctcgctgga tggtcacgac gctaagcagg tgtggacgac cgcgtcgacg  240

gtggacgagg cgctggccca actcgcgatg accgacacgg cgccggccgc ggcttctcgc  300

gccagccgcg tcccgctgtc cgggatggcg ctaccggtcg tcagcgccaa gacggtgcag  360

ctcaacgacg gcgggttggt gcgcacggtg cacttgccgg cccccaatgt cgcggggctg  420

ctgagtgcgg ccggcgtgcc gctgttgcaa agcgaccacg tggtgcccgc cgcgacggcc  480

ccgatcgtcg aaggcatgca gatccaggtg acccgcaatc ggatcaagaa ggtcaccgag  540

cggctgccgc tgccgccgaa cgcgcgtcgt gtcgaggacc cggagatgaa catgagccgg  600

gaggtcgtcg aagacccggg ggttccgggg acccaggatg tgacgttcgc ggtagctgag  660

gtcaacggcg tcgagaccgg ccgtttgccc gtcgccaacg tcgtggtgac cccggcccac  720

gaagccgtgg tgcgggtggg caccaagccc ggtaccgagg tgcccccggt gatcgacgga  780

agcatctggg acgcgatcgc cggctgtgag gccggtggca actgggcgat caacaccggc  840

aacgggtatt acggtggtgt gcagtttgac cagggcacct gggaggccaa cggcgggctg  900

cggtatgcac cccgcgctga cctcgccacc cgcgaagagc agatcgccgt tgccgaggtg  960

acccgactgc gtcaaggttg gggcgcctgg ccggtatgtg ctgcacgagc gggtgcgcgc  1020

tga                                                                1023


<210> 42
<211> 362
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfB (mycobacterial sequence)

<400> 42
Met Leu Arg Leu Val Val Gly Ala Leu Leu Leu Val Leu Ala Phe Ala 
1               5                   10                  15

Gly Gly Tyr Ala Val Ala Ala Cys Lys Thr Val Thr Leu Thr Val Asp 
            20                  25                  30

Gly Thr Ala Met Arg Val Thr Thr Met Lys Ser Arg Val Ile Asp Ile 
        35                  40                  45

Val Glu Glu Asn Gly Phe Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro 
    50                  55                  60

Ala Ala Gly Val Gln Val His Asp Ala Asp Thr Ile Val Leu Arg Arg
65                  70                  75                  80

Ser Arg Pro Leu Gln Ile Ser Leu Asp Gly His Asp Ala Lys Gln Val 
                85                  90                  95

Trp Thr Thr Ala Ser Thr Val Asp Glu Ala Leu Ala Gln Leu Ala Met 
            100                 105                 110

Thr Asp Thr Ala Pro Ala Ala Ala Ser Arg Ala Ser Arg Val Pro Leu 
        115                 120                 125

Ser Gly Met Ala Leu Pro Val Val Ser Ala Lys Thr Val Gln Leu Asn 
    130                 135                 140

Asp Gly Gly Leu Val Arg Thr Val His Leu Pro Ala Pro Asn Val Ala 
145                 150                 155                 160

Gly Leu Leu Ser Ala Ala Gly Val Pro Leu Leu Gln Ser Asp His Val 
                165                 170                 175

Val Pro Ala Ala Thr Ala Pro Ile Val Glu Gly Met Gln Ile Gln Val 
            180                 185                 190

Thr Arg Asn Arg Ile Lys Lys Val Thr Glu Arg Leu Pro Leu Pro Pro 
        195                 200                 205

Asn Ala Arg Arg Val Glu Asp Pro Glu Met Asn Met Ser Arg Glu Val 
    210                 215                 220

Val Glu Asp Pro Gly Val Pro Gly Thr Gln Asp Val Thr Phe Ala Val 
225                 230                 235                 240

Ala Glu Val Asn Gly Val Glu Thr Gly Arg Leu Pro Val Ala Asn Val 
                245                 250                 255

Val Val Thr Pro Ala His Glu Ala Val Val Arg Val Gly Thr Lys Pro 
            260                 265                 270

Gly Thr Glu Val Pro Pro Val Ile Asp Gly Ser Ile Trp Asp Ala Ile 
        275                 280                 285

Ala Gly Cys Glu Ala Gly Gly Asn Trp Ala Ile Asn Thr Gly Asn Gly 
    290                 295                 300

Tyr Tyr Gly Gly Val Gln Phe Asp Gln Gly Thr Trp Glu Ala Asn Gly 
305                 310                 315                 320

Gly Leu Arg Tyr Ala Pro Arg Ala Asp Leu Ala Thr Arg Glu Glu Gln 
                325                 330                 335

Ile Ala Val Ala Glu Val Thr Arg Leu Arg Gln Gly Trp Gly Ala Trp 
            340                 345                 350

Pro Val Cys Ala Ala Arg Ala Gly Ala Arg 
        355                 360


<210> 43
<211> 340
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfB (mycobacterial sequence; 
      signal sequence deleted)

<400> 43
Ala Cys Lys Thr Val Thr Leu Thr Val Asp Gly Thr Ala Met Arg Val 
1               5                   10                  15

Thr Thr Met Lys Ser Arg Val Ile Asp Ile Val Glu Glu Asn Gly Phe 
            20                  25                  30

Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro Ala Ala Gly Val Gln Val 
        35                  40                  45

His Asp Ala Asp Thr Ile Val Leu Arg Arg Ser Arg Pro Leu Gln Ile 
    50                  55                  60

Ser Leu Asp Gly His Asp Ala Lys Gln Val Trp Thr Thr Ala Ser Thr 
65                  70                  75                  80

Val Asp Glu Ala Leu Ala Gln Leu Ala Met Thr Asp Thr Ala Pro Ala 
                85                  90                  95

Ala Ala Ser Arg Ala Ser Arg Val Pro Leu Ser Gly Met Ala Leu Pro 
            100                 105                 110

Val Val Ser Ala Lys Thr Val Gln Leu Asn Asp Gly Gly Leu Val Arg 
        115                 120                 125

Thr Val His Leu Pro Ala Pro Asn Val Ala Gly Leu Leu Ser Ala Ala 
    130                 135                 140

Gly Val Pro Leu Leu Gln Ser Asp His Val Val Pro Ala Ala Thr Ala 
145                 150                 155                 160

Pro Ile Val Glu Gly Met Gln Ile Gln Val Thr Arg Asn Arg Ile Lys 
                165                 170                 175

Lys Val Thr Glu Arg Leu Pro Leu Pro Pro Asn Ala Arg Arg Val Glu 
            180                 185                 190

Asp Pro Glu Met Asn Met Ser Arg Glu Val Val Glu Asp Pro Gly Val 
        195                 200                 205

Pro Gly Thr Gln Asp Val Thr Phe Ala Val Ala Glu Val Asn Gly Val 
    210                 215                 220

Glu Thr Gly Arg Leu Pro Val Ala Asn Val Val Val Thr Pro Ala His 
225                 230                 235                 240

Glu Ala Val Val Arg Val Gly Thr Lys Pro Gly Thr Glu Val Pro Pro 
                245                 250                 255

Val Ile Asp Gly Ser Ile Trp Asp Ala Ile Ala Gly Cys Glu Ala Gly 
            260                 265                 270

Gly Asn Trp Ala Ile Asn Thr Gly Asn Gly Tyr Tyr Gly Gly Val Gln 
        275                 280                 285

Phe Asp Gln Gly Thr Trp Glu Ala Asn Gly Gly Leu Arg Tyr Ala Pro 
    290                 295                 300

Arg Ala Asp Leu Ala Thr Arg Glu Glu Gln Ile Ala Val Ala Glu Val 
305                 310                 315                 320

Thr Arg Leu Arg Gln Gly Trp Gly Ala Trp Pro Val Cys Ala Ala Arg 
                325                 330                 335

Ala Gly Ala Arg 
            340


<210> 44
<211> 465
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2389c

<400> 44
atgacaccgg gtttgcttac tactgcgggt gctggccgac cacgtgacag gtgcgccagg  60

atcgtatgca cggtgttcat cgaaaccgcc gttgtcgcga ccatgtttgt cgcgttgttg  120

ggtctgtcca ccatcagctc gaaagccgac gacatcgatt gggacgccat cgcgcaatgc  180

gaatccggcg gcaattgggc ggccaacacc ggtaacgggt tatacggtgg tctgcagatc  240

agccaggcga cgtgggattc caacggtggt gtcgggtcgc cggcggccgc gagtccccag  300

caacagatcg aggtcgcaga caacattatg aaaacccaag gcccgggtgc gtggccgaaa  360

tgtagttctt gtagtcaggg agacgcaccg ctgggctcgc tcacccacat cctgacgttc  420

ctcgcggccg agactggagg ttgttcgggg agcagggacg attga                  465


<210> 45
<211> 354
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2389c (E. coli 
      optimized; leader sequence deleted)

<400> 45
aagcttttgc tgggcctgag caccattagc agcaaagcgg atgacatcga ctgggatgcg  60

attgcgcagt gtgagagcgg tggcaattgg gcagcgaata ccggcaatgg cctgtacggc  120

ggtctgcaga tctcccaggc gacgtgggac agcaatggtg gcgtcggcag cccggctgcc  180

gcgtccccac aacaacagat cgaggtggca gataacatta tgaaaacgca gggtccgggt  240

gcttggccaa aatgctccag ctgcagccag ggtgacgcac cgctgggcag cctgacccac  300

attctgacgt tcctggcagc ggaaaccggt ggttgtagcg gtagccgcga tgac        354


<210> 46
<211> 465
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2389c (human 
      optimized; leader sequence present)

<400> 46
acccccggac tcctcaccac agctggagct ggcaggccca gagacagatg cgccaggatc  60

gtgtgcaccg tgttcatcga gaccgccgtg gtggctacca tgttcgtggc cctgctgggc  120

ctgagcacca tcagcagcaa ggccgacgac atcgactggg acgccatcgc ccagtgtgaa  180

tccggcggaa actgggccgc caataccggc aatggcctgt acggcggcct gcagatcagc  240

caggctacct gggactccaa cggaggagtg ggaagccctg ccgctgcttc ccctcagcag  300

cagatcgagg tggccgacaa catcatgaag acccaaggcc ctggcgcctg gcctaagtgt  360

tccagctgta gccagggcga tgctcctctg ggcagcctga cccacatcct gacctttctc  420

gccgccgaga caggcggatg tagcggaagc agggacgact aatga                  465


<210> 47
<211> 116
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfD (E. coli optimized)

<400> 47
Leu Leu Gly Leu Ser Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp 
1               5                   10                  15

Asp Ala Ile Ala Gln Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr 
            20                  25                  30

Gly Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp 
        35                  40                  45

Ser Asn Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln 
    50                  55                  60

Ile Glu Val Ala Asp Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp 
65                  70                  75                  80

Pro Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu 
                85                  90                  95

Thr His Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly 
            100                 105                 110

Ser Arg Asp Asp
        115


<210> 48
<211> 153
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfD (mycobacterial sequence 
      and human optimized)

<400> 48
Thr Pro Gly Leu Leu Thr Thr Ala Gly Ala Gly Arg Pro Arg Asp Arg 
1               5                   10                  15

Cys Ala Arg Ile Val Cys Thr Val Phe Ile Glu Thr Ala Val Val Ala 
            20                  25                  30

Thr Met Phe Val Ala Leu Leu Gly Leu Ser Thr Ile Ser Ser Lys Ala 
        35                  40                  45

Asp Asp Ile Asp Trp Asp Ala Ile Ala Gln Cys Glu Ser Gly Gly Asn 
    50                  55                  60

Trp Ala Ala Asn Thr Gly Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser 
65                  70                  75                  80

Gln Ala Thr Trp Asp Ser Asn Gly Gly Val Gly Ser Pro Ala Ala Ala 
                85                  90                  95

Ser Pro Gln Gln Gln Ile Glu Val Ala Asp Asn Ile Met Lys Thr Gln 
            100                 105                 110

Gly Pro Gly Ala Trp Pro Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala 
        115                 120                 125

Pro Leu Gly Ser Leu Thr His Ile Leu Thr Phe Leu Ala Ala Glu Thr 
    130                 135                 140

Gly Gly Cys Ser Gly Ser Arg Asp Asp 
145                 150


<210> 49
<211> 519
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv2450c

<400> 49
ttgaagaacg cccgtacgac gctcatcgcc gccgcgattg ccgggacgtt ggtgaccacg  60

tcaccagccg gtatcgccaa tgccgacgac gcgggcttgg acccaaacgc cgcagccggc  120

ccggatgccg tgggctttga cccgaacctg ccgccggccc cggacgctgc acccgtcgat  180

actccgccgg ctccggagga cgcgggcttt gatcccaacc tccccccgcc gctggccccg  240

gacttcctgt ccccgcctgc ggaggaagcg cctcccgtgc ccgtggccta cagcgtgaac  300

tgggacgcga tcgcgcagtg cgagtccggt ggaaactggt cgatcaacac cggtaacggt  360

tactacggcg gcctgcggtt caccgccggc acctggcgtg ccaacggtgg ctcggggtcc  420

gcggccaacg cgagccggga ggagcagatc cgggtggctg agaacgtgct gcgttcgcag  480

ggtatccgcg cctggccggt ctgcggccgc cgcggctga                         519


<210> 50
<211> 172
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfE 

<400> 50
Leu Lys Asn Ala Arg Thr Thr Leu Ile Ala Ala Ala Ile Ala Gly Thr 
1               5                   10                  15

Leu Val Thr Thr Ser Pro Ala Gly Ile Ala Asn Ala Asp Asp Ala Gly 
            20                  25                  30

Leu Asp Pro Asn Ala Ala Ala Gly Pro Asp Ala Val Gly Phe Asp Pro 
        35                  40                  45

Asn Leu Pro Pro Ala Pro Asp Ala Ala Pro Val Asp Thr Pro Pro Ala 
    50                  55                  60

Pro Glu Asp Ala Gly Phe Asp Pro Asn Leu Pro Pro Pro Leu Ala Pro 
65                  70                  75                  80

Asp Phe Leu Ser Pro Pro Ala Glu Glu Ala Pro Pro Val Pro Val Ala 
                85                  90                  95

Tyr Ser Val Asn Trp Asp Ala Ile Ala Gln Cys Glu Ser Gly Gly Asn 
            100                 105                 110

Trp Ser Ile Asn Thr Gly Asn Gly Tyr Tyr Gly Gly Leu Arg Phe Thr 
        115                 120                 125

Ala Gly Thr Trp Arg Ala Asn Gly Gly Ser Gly Ser Ala Ala Asn Ala 
    130                 135                 140

Ser Arg Glu Glu Gln Ile Arg Val Ala Glu Asn Val Leu Arg Ser Gln 
145                 150                 155                 160

Gly Ile Arg Ala Trp Pro Val Cys Gly Arg Arg Gly 
                165                 170


<210> 51
<211> 1584
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium ESAT6-Rv1733c-
      Rv2626c-RpfD fusion protein (E. coli optimized)

<400> 51
atgacagagc agcagtggaa tttcgcgggt atcgaggccg cggcaagcgc aatccaggga  60

aatgtcacgt ccattcattc cctccttgac gaggggaagc agtccctgac caagctcgca  120

gcggcctggg gcggtagcgg ttcggaggcg taccagggtg tccagcaaaa atgggacgcc  180

acggctaccg agctgaacaa cgcgctgcag aacctggcgc ggacgatcag cgaagccggt  240

caggcaatgg cttcgaccga aggcaacgtc actgggatgt tcgcagaatt catgattgcg  300

actacccgtg atcgtgaggg cgcgaccatg atcacgttcc gtctgcgtct gccgtgtcgc  360

accattttgc gcgtgttttc gcgtaacccg ctggtccgcg gtaccgaccg tctggaggcc  420

cccggggtcc aagacagccg tagccatgtg tatgctcacc aggctcaaac ccgtcacccg  480

gctactgcca ctgttatcga tcacgaaggc gtgattgact ccaataccac ggcaacctcc  540

gcaccgcctc gcaccaagat tacggttcct gcgcgttggg tggtgaatgg tattgaacgc  600

agcggcgaag ttaatgccaa accgggtacc aaaagcggtg accgtgtggg catctgggtc  660

gatagcgccg gtcagctggt cgacgagccg gcaccgccag cgcgtgcgat cgccgattct  720

agacgcgcaa ttctgatccg cgttcgcaat gcgagctggc agcacgatat tgatagcctg  780

ttttgcaccc aacgtgagct catgaccacg gcgcgtgata tcatgaatgc gggtgtcacc  840

tgtgttggcg agcacgaaac gttgaccgca gcagcacagt acatgcgcga acatgatatc  900

ggcgcattgc cgatttgcgg cgacgatgat cgtctgcacg gtatgctgac cgaccgcgat  960

atcgttatca agggtctggc cgcaggcttg gacccgaaca ccgcgaccgc cggtgaactg  1020

gcacgtgaca gcatctatta cgtcgacgcg aacgccagca ttcaagagat gctgaacgtg  1080

atggaagagc atcaggtgcg tcgtgtcccg gttatcagcg aacatcgtct ggttggtatc  1140

gttaccgaag ccgacatcgc acgtcacctg ccggagcacg cgattgttca gttcgtgaaa  1200

gcgatttgca gcccgatggc gttggcgtct aagcttttgc tgggcctgag caccattagc  1260

agcaaagcgg atgacatcga ctgggatgcg attgcgcagt gtgagagcgg tggcaattgg  1320

gcagcgaata ccggcaatgg cctgtacggc ggtctgcaga tctcccaggc gacgtgggac  1380

agcaatggtg gcgtcggcag cccggctgcc gcgtccccac aacaacagat cgaggtggca  1440

gataacatta tgaaaacgca gggtccgggt gcttggccaa aatgctccag ctgcagccag  1500

ggtgacgcac cgctgggcag cctgacccac attctgacgt tcctggcagc ggaaaccggt  1560

ggttgtagcg gtagccgcga tgac                                         1584


<210> 52
<211> 1689
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium ESAT6-Rv1733c-
      Rv2626c-RpfD fusion protein (human optimized)

<400> 52
atgaccgagc agcagtggaa cttcgccggc atcgaagctg ccgctagcgc catccaaggc  60

aacgtgacca gcatccacag cctgctggac gagggcaagc agagcctgac caagctggct  120

gctgcttggg gcggatccgg aagcgaagcc taccagggcg tgcagcagaa gtgggacgcc  180

acagccaccg agctgaacaa cgccctgcag aacctcgcca gaaccatcag cgaggccgga  240

caggctatgg ccagcacaga gggcaatgtg accggcatgt tcgccttcga aatcgccacc  300

accagggaca gggaaggcgc taccatgatc accttcaggc tgaggctccc ctgcaggacc  360

atcctgaggg tgttcagcag gaaccccctg gtgaggggca ccgacagact ggaagccgtg  420

caggacagca ggagccacgt gtatgcccac caggctcaga ccaggcaccc tgctaccgcc  480

accgtgatcg accacgaggg cgtgatcgac tccaacacca ccgccaccag cgctcctccc  540

agaaccaaga tcacagtgcc cgccaggtgg gtggtgaacg gcatcgagag gagcggcgag  600

gtgaacgcca agcctggaac caagagcggc gacagggtgg gcatttgggt cgatagcgcc  660

ggccagctgg tggatgaacc tgctccccct gccagagcca tcgccgatag ggccatcctg  720

atcagggtga ggaacgccag ctggcagcac gacatcgaca gcctgttctg cacccaaagg  780

cgatcgacaa cagccaggga catcatgaac gccggcgtga cctgcgtggg agagcatgaa  840

accctcaccg ccgccgccca atacatgagg gagcacgaca tcggcgccct gcccatctgt  900

ggagacgacg acaggctgca cggcatgctg accgacaggg acatcgtgat caagggcctg  960

gctgccggcc tcgatcctaa caccgctaca gccggcgagc tggccagaga cagcatctac  1020

tacgtggacg ccaacgccag catccaggag atgctcaacg tgatggagga gcaccaggtg  1080

agaagggtgc ctgtgatcag cgagcacagg ctggtgggca tcgtgaccga ggccgatatc  1140

gctaggcacc tgcccgagca cgccatcgtg cagttcgtga aggccatctg cagccccatg  1200

gctctggcca gcggcgcgcc cacccccgga ctcctcacca cagctggagc tggcaggccc  1260

agagacagat gcgccaggat cgtgtgcacc gtgttcatcg agaccgccgt ggtggctacc  1320

atgttcgtgg ccctgctggg cctgagcacc atcagcagca aggccgacga catcgactgg  1380

gacgccatcg cccagtgtga atccggcgga aactgggccg ccaataccgg caatggcctg  1440

tacggcggcc tgcagatcag ccaggctacc tgggactcca acggaggagt gggaagccct  1500

gccgctgctt cccctcagca gcagatcgag gtggccgaca acatcatgaa gacccaaggc  1560

cctggcgcct ggcctaagtg ttccagctgt agccagggcg atgctcctct gggcagcctg  1620

acccacatcc tgacctttct cgccgccgag acaggcggat gtagcggaag cagggacgac  1680

taatgatag                                                          1689


<210> 53
<211> 528
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT6-Rv1733c-Rv2626c-RpfD 
      (E. coli optimized)

<400> 53
Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
1               5                   10                  15

Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
            20                  25                  30

Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
        35                  40                  45

Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
    50                  55                  60

Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly 
65                  70                  75                  80

Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala Glu 
                85                  90                  95

Phe Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr 
            100                 105                 110

Phe Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg 
        115                 120                 125

Asn Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Pro Gly Val Gln 
    130                 135                 140

Asp Ser Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro 
145                 150                 155                 160

Ala Thr Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr 
                165                 170                 175

Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg 
            180                 185                 190

Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro 
        195                 200                 205

Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly 
    210                 215                 220

Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Ser 
225                 230                 235                 240

Arg Arg Ala Ile Leu Ile Arg Val Arg Asn Ala Ser Trp Gln His Asp 
                245                 250                 255

Ile Asp Ser Leu Phe Cys Thr Gln Arg Glu Leu Met Thr Thr Ala Arg 
            260                 265                 270

Asp Ile Met Asn Ala Gly Val Thr Cys Val Gly Glu His Glu Thr Leu 
        275                 280                 285

Thr Ala Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly Ala Leu Pro 
    290                 295                 300

Ile Cys Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr Asp Arg Asp 
305                 310                 315                 320

Ile Val Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn Thr Ala Thr 
                325                 330                 335

Ala Gly Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp Ala Asn Ala 
            340                 345                 350

Ser Ile Gln Glu Met Leu Asn Val Met Glu Glu His Gln Val Arg Arg 
        355                 360                 365

Val Pro Val Ile Ser Glu His Arg Leu Val Gly Ile Val Thr Glu Ala 
    370                 375                 380

Asp Ile Ala Arg His Leu Pro Glu His Ala Ile Val Gln Phe Val Lys 
385                 390                 395                 400

Ala Ile Cys Ser Pro Met Ala Leu Ala Ser Lys Leu Leu Leu Gly Leu 
                405                 410                 415

Ser Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp Asp Ala Ile Ala 
            420                 425                 430

Gln Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu 
        435                 440                 445

Tyr Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp Ser Asn Gly Gly 
    450                 455                 460

Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu Val Ala 
465                 470                 475                 480

Asp Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp Pro Lys Cys Ser 
                485                 490                 495

Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His Ile Leu 
            500                 505                 510

Thr Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly Ser Arg Asp Asp 
        515                 520                 525


<210> 54
<211> 559
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT6-Rv1733c-Rv2626c-RpfD 
      (human optimized)

<400> 54
Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
1               5                   10                  15

Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
            20                  25                  30

Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
        35                  40                  45

Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
    50                  55                  60

Leu Asn Asn Ala Leu Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln 
65                  70                  75                  80

Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala Phe Glu 
                85                  90                  95

Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe Arg 
            100                 105                 110

Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn Pro 
        115                 120                 125

Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Val Gln Asp Ser Arg Ser 
    130                 135                 140

His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro Ala Thr Ala Thr 
145                 150                 155                 160

Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr Thr Ala Thr Ser 
                165                 170                 175

Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg Trp Val Val Asn 
            180                 185                 190

Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro Gly Thr Lys Ser 
        195                 200                 205

Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly Gln Leu Val Asp 
    210                 215                 220

Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Arg Ala Ile Leu Ile 
225                 230                 235                 240

Arg Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys 
                245                 250                 255

Thr Gln Arg Arg Ser Thr Thr Ala Arg Asp Ile Met Asn Ala Gly Val 
            260                 265                 270

Thr Cys Val Gly Glu His Glu Thr Leu Thr Ala Ala Ala Gln Tyr Met 
        275                 280                 285

Arg Glu His Asp Ile Gly Ala Leu Pro Ile Cys Gly Asp Asp Asp Arg 
    290                 295                 300

Leu His Gly Met Leu Thr Asp Arg Asp Ile Val Ile Lys Gly Leu Ala 
305                 310                 315                 320

Ala Gly Leu Asp Pro Asn Thr Ala Thr Ala Gly Glu Leu Ala Arg Asp 
                325                 330                 335

Ser Ile Tyr Tyr Val Asp Ala Asn Ala Ser Ile Gln Glu Met Leu Asn 
            340                 345                 350

Val Met Glu Glu His Gln Val Arg Arg Val Pro Val Ile Ser Glu His 
        355                 360                 365

Arg Leu Val Gly Ile Val Thr Glu Ala Asp Ile Ala Arg His Leu Pro 
    370                 375                 380

Glu His Ala Ile Val Gln Phe Val Lys Ala Ile Cys Ser Pro Met Ala 
385                 390                 395                 400

Leu Ala Ser Gly Ala Pro Thr Pro Gly Leu Leu Thr Thr Ala Gly Ala 
                405                 410                 415

Gly Arg Pro Arg Asp Arg Cys Ala Arg Ile Val Cys Thr Val Phe Ile 
            420                 425                 430

Glu Thr Ala Val Val Ala Thr Met Phe Val Ala Leu Leu Gly Leu Ser 
        435                 440                 445

Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp Asp Ala Ile Ala Gln 
    450                 455                 460

Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu Tyr 
465                 470                 475                 480

Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp Ser Asn Gly Gly Val 
                485                 490                 495

Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu Val Ala Asp 
            500                 505                 510

Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp Pro Lys Cys Ser Ser 
        515                 520                 525

Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His Ile Leu Thr 
    530                 535                 540

Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly Ser Arg Asp Asp
545                 550                 555


<210> 55
<211> 2421
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium ESAT6-Rv1733c-
      Rv2626c-RpfB fusion protein

<400> 55
atgacagagc agcagtggaa tttcgcgggt atcgaggccg cggcaagcgc aatccaggga  60

aatgtcacgt ccattcattc cctccttgac gaggggaagc agtccctgac caagctcgca  120

gcggcctggg gcggtagcgg ttcggaggcg taccagggtg tccagcaaaa atgggacgcc  180

acggctaccg agctgaacaa cgcgctgcag aacctggcgc ggacgatcag cgaagccggt  240

caggcaatgg cttcgaccga aggcaacgtc actgggatgt tcgcagaatt catgattgcg  300

actacccgtg atcgtgaggg cgcgaccatg atcacgttcc gtctgcgtct gccgtgtcgc  360

accattttgc gcgtgttttc gcgtaacccg ctggtccgcg gtaccgaccg tctggaggcc  420

gttgtcatgc tgctggcggt taccgtgagc ctgctgacga tcccattcgc agcggcagct  480

ggcacggccg tccaagacag ccgtagccat gtgtatgctc accaggctca aacccgtcac  540

ccggctactg ccactgttat cgatcacgaa ggcgtgattg actccaatac cacggcaacc  600

tccgcaccgc ctcgcaccaa gattacggtt cctgcgcgtt gggtggtgaa tggtattgaa  660

cgcagcggcg aagttaatgc caaaccgggt accaaaagcg gtgaccgtgt gggcatctgg  720

gtcgatagcg ccggtcagct ggtcgacgag ccggcaccgc cagcgcgtgc gatcgccgat  780

gcggcgctgg ctgccctggg tctgtggctg agcgtggcag cggtcgccgg tgcgttgctg  840

gcgctgacgc gcgcaattct gatccgcgtt cgcaatgcga gctggcagca cgatattgat  900

agcctgtttt gcacccaacg tgagctcatg accacggcgc gtgatatcat gaatgcgggt  960

gtcacctgtg ttggcgagca cgaaacgttg accgcagcag cacagtacat gcgcgaacat  1020

gatatcggcg cattgccgat ttgcggcgac gatgatcgtc tgcacggtat gctgaccgac  1080

cgcgatatcg ttatcaaggg tctggccgca ggcttggacc cgaacaccgc gaccgccggt  1140

gaactggcac gtgacagcat ctattacgtc gacgcgaacg ccagcattca agagatgctg  1200

aacgtgatgg aagagcatca ggtgcgtcgt gtcccggtta tcagcgaaca tcgtctggtt  1260

ggtatcgtta ccgaagccga catcgcacgt cacctgccgg agcacgcgat tgttcagttc  1320

gtgaaagcga tttgcagccc gatggcgttg gcgtctcgtc aaaagggcga cacaaaattt  1380

attctaaatg caaagcttgc atgcaaaacg gtgacgttga ccgtcgacgg aaccgcgatg  1440

cgggtgacca cgatgaaatc gcgggtgatc gacatcgtcg aagagaacgg gttctcagtc  1500

gacgaccgcg acgacctgta tcccgcggcc ggcgtgcagg tccatgacgc cgacaccatc  1560

gtgctgcggc gtagccgtcc gctgcagatc tcgctggatg gtcacgacgc taagcaggtg  1620

tggacgaccg cgtcgacggt ggacgaggcg ctggcccaac tcgcgatgac cgacacggcg  1680

ccggccgcgg cttctcgcgc cagccgcgtc ccgctgtccg ggatggcgct accggtcgtc  1740

agcgccaaga cggtgcagct caacgacggc gggttggtgc gcacggtgca cttgccggcc  1800

cccaatgtcg cggggctgct gagtgcggcc ggcgtgccgc tgttgcaaag cgaccacgtg  1860

gtgcccgccg cgacggcccc gatcgtcgaa ggcatgcaga tccaggtgac ccgcaatcgg  1920

atcaagaagg tcaccgagcg gctgccgctg ccgccgaacg cgcgtcgtgt cgaggacccg  1980

gagatgaaca tgagccggga ggtcgtcgaa gacccggggg ttccggggac ccaggatgtg  2040

acgttcgcgg tagctgaggt caacggcgtc gagaccggcc gtttgcccgt cgccaacgtc  2100

gtggtgaccc cggcccacga agccgtggtg cgggtgggca ccaagcccgg taccgaggtg  2160

cccccggtga tcgacggaag catctgggac gcgatcgccg gctgtgaggc cggtggcaac  2220

tgggcgatca acaccggcaa cgggtattac ggtggtgtgc agtttgacca gggcacctgg  2280

gaggccaacg gcgggctgcg gtatgcaccc cgcgctgacc tcgccacccg cgaagagcag  2340

atcgccgttg ccgaggtgac ccgactgcgt caaggttggg gcgcctggcc ggtatgtgct  2400

gcacgagcgg gtgcgcgctg a                                            2421


<210> 56
<211> 806
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT6-Rv1733c-Rv2626c-RpfB 

<400> 56
Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
1               5                   10                  15

Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
            20                  25                  30

Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
        35                  40                  45

Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
    50                  55                  60

Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly 
65                  70                  75                  80

Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala Glu 
                85                  90                  95

Phe Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr 
            100                 105                 110

Phe Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg 
        115                 120                 125

Asn Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Val Val Met Leu 
    130                 135                 140

Leu Ala Val Thr Val Ser Leu Leu Thr Ile Pro Phe Ala Ala Ala Ala 
145                 150                 155                 160

Gly Thr Ala Val Gln Asp Ser Arg Ser His Val Tyr Ala His Gln Ala 
                165                 170                 175

Gln Thr Arg His Pro Ala Thr Ala Thr Val Ile Asp His Glu Gly Val 
            180                 185                 190

Ile Asp Ser Asn Thr Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile 
        195                 200                 205

Thr Val Pro Ala Arg Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu 
    210                 215                 220

Val Asn Ala Lys Pro Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp 
225                 230                 235                 240

Val Asp Ser Ala Gly Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg 
                245                 250                 255

Ala Ile Ala Asp Ala Ala Leu Ala Ala Leu Gly Leu Trp Leu Ser Val 
            260                 265                 270

Ala Ala Val Ala Gly Ala Leu Leu Ala Leu Thr Arg Ala Ile Leu Ile 
        275                 280                 285

Arg Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys 
    290                 295                 300

Thr Gln Arg Glu Leu Met Thr Thr Ala Arg Asp Ile Met Asn Ala Gly 
305                 310                 315                 320

Val Thr Cys Val Gly Glu His Glu Thr Leu Thr Ala Ala Ala Gln Tyr 
                325                 330                 335

Met Arg Glu His Asp Ile Gly Ala Leu Pro Ile Cys Gly Asp Asp Asp 
            340                 345                 350

Arg Leu His Gly Met Leu Thr Asp Arg Asp Ile Val Ile Lys Gly Leu 
        355                 360                 365

Ala Ala Gly Leu Asp Pro Asn Thr Ala Thr Ala Gly Glu Leu Ala Arg 
    370                 375                 380

Asp Ser Ile Tyr Tyr Val Asp Ala Asn Ala Ser Ile Gln Glu Met Leu 
385                 390                 395                 400

Asn Val Met Glu Glu His Gln Val Arg Arg Val Pro Val Ile Ser Glu 
                405                 410                 415

His Arg Leu Val Gly Ile Val Thr Glu Ala Asp Ile Ala Arg His Leu 
            420                 425                 430

Pro Glu His Ala Ile Val Gln Phe Val Lys Ala Ile Cys Ser Pro Met 
        435                 440                 445

Ala Leu Ala Ser Arg Gln Lys Gly Asp Thr Lys Phe Ile Leu Asn Ala 
    450                 455                 460

Lys Leu Ala Cys Lys Thr Val Thr Leu Thr Val Asp Gly Thr Ala Met 
465                 470                 475                 480

Arg Val Thr Thr Met Lys Ser Arg Val Ile Asp Ile Val Glu Glu Asn 
                485                 490                 495

Gly Phe Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro Ala Ala Gly Val 
            500                 505                 510

Gln Val His Asp Ala Asp Thr Ile Val Leu Arg Arg Ser Arg Pro Leu 
        515                 520                 525

Gln Ile Ser Leu Asp Gly His Asp Ala Lys Gln Val Trp Thr Thr Ala 
    530                 535                 540

Ser Thr Val Asp Glu Ala Leu Ala Gln Leu Ala Met Thr Asp Thr Ala 
545                 550                 555                 560

Pro Ala Ala Ala Ser Arg Ala Ser Arg Val Pro Leu Ser Gly Met Ala 
                565                 570                 575

Leu Pro Val Val Ser Ala Lys Thr Val Gln Leu Asn Asp Gly Gly Leu 
            580                 585                 590

Val Arg Thr Val His Leu Pro Ala Pro Asn Val Ala Gly Leu Leu Ser 
        595                 600                 605

Ala Ala Gly Val Pro Leu Leu Gln Ser Asp His Val Val Pro Ala Ala 
    610                 615                 620

Thr Ala Pro Ile Val Glu Gly Met Gln Ile Gln Val Thr Arg Asn Arg 
625                 630                 635                 640

Ile Lys Lys Val Thr Glu Arg Leu Pro Leu Pro Pro Asn Ala Arg Arg 
                645                 650                 655

Val Glu Asp Pro Glu Met Asn Met Ser Arg Glu Val Val Glu Asp Pro 
            660                 665                 670

Gly Val Pro Gly Thr Gln Asp Val Thr Phe Ala Val Ala Glu Val Asn 
        675                 680                 685

Gly Val Glu Thr Gly Arg Leu Pro Val Ala Asn Val Val Val Thr Pro 
    690                 695                 700

Ala His Glu Ala Val Val Arg Val Gly Thr Lys Pro Gly Thr Glu Val 
705                 710                 715                 720

Pro Pro Val Ile Asp Gly Ser Ile Trp Asp Ala Ile Ala Gly Cys Glu 
                725                 730                 735

Ala Gly Gly Asn Trp Ala Ile Asn Thr Gly Asn Gly Tyr Tyr Gly Gly 
            740                 745                 750

Val Gln Phe Asp Gln Gly Thr Trp Glu Ala Asn Gly Gly Leu Arg Tyr 
        755                 760                 765

Ala Pro Arg Ala Asp Leu Ala Thr Arg Glu Glu Gln Ile Ala Val Ala 
    770                 775                 780

Glu Val Thr Arg Leu Arg Gln Gly Trp Gly Ala Trp Pro Val Cys Ala 
785                 790                 795                 800

Ala Arg Ala Gly Ala Arg 
                805


<210> 57
<211> 2430
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium RpfB-ESAT6-
      Rv1733c-Rv2626c fusion protein

<400> 57
atgaagcttg catgcaaaac ggtgacgttg accgtcgacg gaaccgcgat gcgggtgacc  60

acgatgaaat cgcgggtgat cgacatcgtc gaagagaacg ggttctcagt cgacgaccgc  120

gacgacctgt atcccgcggc cggcgtgcag gtccatgacg ccgacaccat cgtgctgcgg  180

cgtagccgtc cgctgcagat ctcgctggat ggtcacgacg ctaagcaggt gtggacgacc  240

gcgtcgacgg tggacgaggc gctggcccaa ctcgcgatga ccgacacggc gccggccgcg  300

gcttctcgcg ccagccgcgt cccgctgtcc gggatggcgc taccggtcgt cagcgccaag  360

acggtgcagc tcaacgacgg cgggttggtg cgcacggtgc acttgccggc ccccaatgtc  420

gcggggctgc tgagtgcggc cggcgtgccg ctgttgcaaa gcgaccacgt ggtgcccgcc  480

gcgacggccc cgatcgtcga aggcatgcag atccaggtga cccgcaatcg gatcaagaag  540

gtcaccgagc ggctgccgct gccgccgaac gcgcgtcgtg tcgaggaccc ggagatgaac  600

atgagccggg aggtcgtcga agacccgggg gttccgggga cccaggatgt gacgttcgcg  660

gtagctgagg tcaacggcgt cgagaccggc cgtttgcccg tcgccaacgt cgtggtgacc  720

ccggcccacg aagccgtggt gcgggtgggc accaagcccg gtaccgaggt gcccccggtg  780

atcgacggaa gcatctggga cgcgatcgcc ggctgtgagg ccggtggcaa ctgggcgatc  840

aacaccggca acgggtatta cggtggtgtg cagtttgacc agggcacctg ggaggccaac  900

ggcgggctgc ggtatgcacc ccgcgctgac ctcgccaccc gcgaagagca gatcgccgtt  960

gccgaggtga cccgactgcg tcaaggttgg ggcgcctggc cggtatgtgc tgcacgagcg  1020

ggtgcgcgcg gatccatgac agagcagcag tggaatttcg cgggtatcga ggccgcggca  1080

agcgcaatcc agggaaatgt cacgtccatt cattccctcc ttgacgaggg gaagcagtcc  1140

ctgaccaagc tcgcagcggc ctggggcggt agcggttcgg aggcgtacca gggtgtccag  1200

caaaaatggg acgccacggc taccgagctg aacaacgcgc tgcagaacct ggcgcggacg  1260

atcagcgaag ccggtcaggc aatggcttcg accgaaggca acgtcactgg gatgttcgca  1320

gaattcatga ttgcgactac ccgtgatcgt gagggcgcga ccatgatcac gttccgtctg  1380

cgtctgccgt gtcgcaccat tttgcgcgtg ttttcgcgta acccgctggt ccgcggtacc  1440

gaccgtctgg aggccgttgt catgctgctg gcggttaccg tgagcctgct gacgatccca  1500

ttcgcagcgg cagctggcac ggccgtccaa gacagccgta gccatgtgta tgctcaccag  1560

gctcaaaccc gtcacccggc tactgccact gttatcgatc acgaaggcgt gattgactcc  1620

aataccacgg caacctccgc accgcctcgc accaagatta cggttcctgc gcgttgggtg  1680

gtgaatggta ttgaacgcag cggcgaagtt aatgccaaac cgggtaccaa aagcggtgac  1740

cgtgtgggca tctgggtcga tagcgccggt cagctggtcg acgagccggc accgccagcg  1800

cgtgcgatcg ccgatgcggc gctggctgcc ctgggtctgt ggctgagcgt ggcagcggtc  1860

gccggtgcgt tgctggcgct gacgcgcgca attctgatcc gcgttcgcaa tgcgagctgg  1920

cagcacgata ttgatagcct gttttgcacc caacgtgagc tcatgaccac ggcgcgtgat  1980

atcatgaatg cgggtgtcac ctgtgttggc gagcacgaaa cgttgaccgc agcagcacag  2040

tacatgcgcg aacatgatat cggcgcattg ccgatttgcg gcgacgatga tcgtctgcac  2100

ggtatgctga ccgaccgcga tatcgttatc aagggtctgg ccgcaggctt ggacccgaac  2160

accgcgaccg ccggtgaact ggcacgtgac agcatctatt acgtcgacgc gaacgccagc  2220

attcaagaga tgctgaacgt gatggaagag catcaggtgc gtcgtgtccc ggttatcagc  2280

gaacatcgtc tggttggtat cgttaccgaa gccgacatcg cacgtcacct gccggagcac  2340

gcgattgttc agttcgtgaa agcgatttgc agcccgatgg cgttggcgtc tcgtcaaaag  2400

ggcgacacaa aatttattct aaatgcatga                                   2430


<210> 58
<211> 809
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfB-ESAT6-Rv1733c-Rv2626c 

<400> 58
Met Lys Leu Ala Cys Lys Thr Val Thr Leu Thr Val Asp Gly Thr Ala 
1               5                   10                  15

Met Arg Val Thr Thr Met Lys Ser Arg Val Ile Asp Ile Val Glu Glu 
            20                  25                  30

Asn Gly Phe Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro Ala Ala Gly 
        35                  40                  45

Val Gln Val His Asp Ala Asp Thr Ile Val Leu Arg Arg Ser Arg Pro 
    50                  55                  60

Leu Gln Ile Ser Leu Asp Gly His Asp Ala Lys Gln Val Trp Thr Thr 
65                  70                  75                  80

Ala Ser Thr Val Asp Glu Ala Leu Ala Gln Leu Ala Met Thr Asp Thr 
                85                  90                  95

Ala Pro Ala Ala Ala Ser Arg Ala Ser Arg Val Pro Leu Ser Gly Met 
            100                 105                 110

Ala Leu Pro Val Val Ser Ala Lys Thr Val Gln Leu Asn Asp Gly Gly 
        115                 120                 125

Leu Val Arg Thr Val His Leu Pro Ala Pro Asn Val Ala Gly Leu Leu 
    130                 135                 140

Ser Ala Ala Gly Val Pro Leu Leu Gln Ser Asp His Val Val Pro Ala 
145                 150                 155                 160

Ala Thr Ala Pro Ile Val Glu Gly Met Gln Ile Gln Val Thr Arg Asn 
                165                 170                 175

Arg Ile Lys Lys Val Thr Glu Arg Leu Pro Leu Pro Pro Asn Ala Arg 
            180                 185                 190

Arg Val Glu Asp Pro Glu Met Asn Met Ser Arg Glu Val Val Glu Asp 
        195                 200                 205

Pro Gly Val Pro Gly Thr Gln Asp Val Thr Phe Ala Val Ala Glu Val 
    210                 215                 220

Asn Gly Val Glu Thr Gly Arg Leu Pro Val Ala Asn Val Val Val Thr 
225                 230                 235                 240

Pro Ala His Glu Ala Val Val Arg Val Gly Thr Lys Pro Gly Thr Glu 
                245                 250                 255

Val Pro Pro Val Ile Asp Gly Ser Ile Trp Asp Ala Ile Ala Gly Cys 
            260                 265                 270

Glu Ala Gly Gly Asn Trp Ala Ile Asn Thr Gly Asn Gly Tyr Tyr Gly 
        275                 280                 285

Gly Val Gln Phe Asp Gln Gly Thr Trp Glu Ala Asn Gly Gly Leu Arg 
    290                 295                 300

Tyr Ala Pro Arg Ala Asp Leu Ala Thr Arg Glu Glu Gln Ile Ala Val 
305                 310                 315                 320

Ala Glu Val Thr Arg Leu Arg Gln Gly Trp Gly Ala Trp Pro Val Cys 
                325                 330                 335

Ala Ala Arg Ala Gly Ala Arg Gly Ser Met Thr Glu Gln Gln Trp Asn 
            340                 345                 350

Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr 
        355                 360                 365

Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu 
    370                 375                 380

Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln 
385                 390                 395                 400

Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn 
                405                 410                 415

Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu 
            420                 425                 430

Gly Asn Val Thr Gly Met Phe Ala Glu Phe Met Ile Ala Thr Thr Arg 
        435                 440                 445

Asp Arg Glu Gly Ala Thr Met Ile Thr Phe Arg Leu Arg Leu Pro Cys 
    450                 455                 460

Arg Thr Ile Leu Arg Val Phe Ser Arg Asn Pro Leu Val Arg Gly Thr 
465                 470                 475                 480

Asp Arg Leu Glu Ala Val Val Met Leu Leu Ala Val Thr Val Ser Leu 
                485                 490                 495

Leu Thr Ile Pro Phe Ala Ala Ala Ala Gly Thr Ala Val Gln Asp Ser 
            500                 505                 510

Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro Ala Thr 
        515                 520                 525

Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr Thr Ala 
    530                 535                 540

Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg Trp Val 
545                 550                 555                 560

Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro Gly Thr 
                565                 570                 575

Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly Gln Leu 
            580                 585                 590

Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Ala Ala Leu 
        595                 600                 605

Ala Ala Leu Gly Leu Trp Leu Ser Val Ala Ala Val Ala Gly Ala Leu 
    610                 615                 620

Leu Ala Leu Thr Arg Ala Ile Leu Ile Arg Val Arg Asn Ala Ser Trp 
625                 630                 635                 640

Gln His Asp Ile Asp Ser Leu Phe Cys Thr Gln Arg Glu Leu Met Thr 
                645                 650                 655

Thr Ala Arg Asp Ile Met Asn Ala Gly Val Thr Cys Val Gly Glu His 
            660                 665                 670

Glu Thr Leu Thr Ala Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly 
        675                 680                 685

Ala Leu Pro Ile Cys Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr 
    690                 695                 700

Asp Arg Asp Ile Val Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn 
705                 710                 715                 720

Thr Ala Thr Ala Gly Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp 
                725                 730                 735

Ala Asn Ala Ser Ile Gln Glu Met Leu Asn Val Met Glu Glu His Gln 
            740                 745                 750

Val Arg Arg Val Pro Val Ile Ser Glu His Arg Leu Val Gly Ile Val 
        755                 760                 765

Thr Glu Ala Asp Ile Ala Arg His Leu Pro Glu His Ala Ile Val Gln 
    770                 775                 780

Phe Val Lys Ala Ile Cys Ser Pro Met Ala Leu Ala Ser Arg Gln Lys 
785                 790                 795                 800

Gly Asp Thr Lys Phe Ile Leu Asn Ala 
                805


<210> 59
<211> 2448
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Ag85B-ESAT6-
      Rv1733c-Rv2626c-RpfD fusion protein (E. coli optimized)

<400> 59
atgtttagcc gtcctggcct gccagttgaa tacctgcaag ttccgagccc gtccatgggt  60

cgtgacatta aggtgcagtt ccagagcggc ggtaacaata gcccggctgt gtacctgctg  120

gacggtctgc gtgcgcagga tgattacaac ggctgggaca tcaatacccc ggcatttgag  180

tggtattacc agtcgggtct gagcattgtg atgccggttg gcggtcaaag cagcttctat  240

agcgattggt acagcccggc atgcggcaag gctggttgcc aaacctacaa gtgggaaact  300

ttcttgacca gcgagctgcc gcaatggttg agcgccaacc gtgcggtcaa accgaccggt  360

agcgctgcta ttggcctgtc catggccggc agcagcgcga tgatcttggc ggcataccat  420

ccgcagcagt ttatctacgc cggtagcctg agcgcattgc tggacccgag ccaaggcatg  480

ggtccgagcc tgattggtct ggcaatgggt gacgcaggtg gttacaaagc ggccgatatg  540

tggggcccat ctagcgaccc ggcatgggag cgtaatgacc cgacccagca aattccgaaa  600

ctggtggcga ataacacgcg cctgtgggtc tactgtggca atggtacgcc gaacgagctg  660

ggtggcgcga atatccctgc ggagtttctg gaaaactttg ttcgcagcag caacctgaaa  720

ttccaggacg cgtataacgc agccggtggt cacaatgcgg ttttcaattt cccgccaaat  780

ggcactcata gctgggagta ctggggtgcg cagttgaacg caatgaaagg cgatctgcaa  840

tcctctctgg gtgcgggcgg atccatgaca gagcagcagt ggaatttcgc gggtatcgag  900

gccgcggcaa gcgcaatcca gggaaatgtc acgtccattc attccctcct tgacgagggg  960

aagcagtccc tgaccaagct cgcagcggcc tggggcggta gcggttcgga ggcgtaccag  1020

ggtgtccagc aaaaatggga cgccacggct accgagctga acaacgcgct gcagaacctg  1080

gcgcggacga tcagcgaagc cggtcaggca atggcttcga ccgaaggcaa cgtcactggg  1140

atgttcgcag aattcatgat tgcgactacc cgtgatcgtg agggcgcgac catgatcacg  1200

ttccgtctgc gtctgccgtg tcgcaccatt ttgcgcgtgt tttcgcgtaa cccgctggtc  1260

cgcggtaccg accgtctgga ggcccccggg gtccaagaca gccgtagcca tgtgtatgct  1320

caccaggctc aaacccgtca cccggctact gccactgtta tcgatcacga aggcgtgatt  1380

gactccaata ccacggcaac ctccgcaccg cctcgcacca agattacggt tcctgcgcgt  1440

tgggtggtga atggtattga acgcagcggc gaagttaatg ccaaaccggg taccaaaagc  1500

ggtgaccgtg tgggcatctg ggtcgatagc gccggtcagc tggtcgacga gccggcaccg  1560

ccagcgcgtg cgatcgccga ttctagacgc gcaattctga tccgcgttcg caatgcgagc  1620

tggcagcacg atattgatag cctgttttgc acccaacgtg agctcatgac cacggcgcgt  1680

gatatcatga atgcgggtgt cacctgtgtt ggcgagcacg aaacgttgac cgcagcagca  1740

cagtacatgc gcgaacatga tatcggcgca ttgccgattt gcggcgacga tgatcgtctg  1800

cacggtatgc tgaccgaccg cgatatcgtt atcaagggtc tggccgcagg cttggacccg  1860

aacaccgcga ccgccggtga actggcacgt gacagcatct attacgtcga cgcgaacgcc  1920

agcattcaag agatgctgaa cgtgatggaa gagcatcagg tgcgtcgtgt cccggttatc  1980

agcgaacatc gtctggttgg tatcgttacc gaagccgaca tcgcacgtca cctgccggag  2040

cacgcgattg ttcagttcgt gaaagcgatt tgcagcccga tggcgttggc gtctaagctt  2100

ttgctgggcc tgagcaccat tagcagcaaa gcggatgaca tcgactggga tgcgattgcg  2160

cagtgtgaga gcggtggcaa ttgggcagcg aataccggca atggcctgta cggcggtctg  2220

cagatctccc aggcgacgtg ggacagcaat ggtggcgtcg gcagcccggc tgccgcgtcc  2280

ccacaacaac agatcgaggt ggcagataac attatgaaaa cgcagggtcc gggtgcttgg  2340

ccaaaatgct ccagctgcag ccagggtgac gcaccgctgg gcagcctgac ccacattctg  2400

acgttcctgg cagcggaaac cggtggttgt agcggtagcc gcgatgac               2448


<210> 60
<211> 2550
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Ag85B-ESAT6-
      Rv1733c-Rv2626c-RpfD fusion protein (human optimized)

<400> 60
atgttctcca ggcccggcct gcctgtcgag tatctgcagg tcccctcccc ctccatgggc  60

agagacatca aggtgcagtt ccaatccgga ggcaacaaca gccccgccgt gtatctcctc  120

gacggcctga gggctcagga cgactacaac ggctgggaca tcaacacccc cgccttcgag  180

tggtactacc agtccggact gagcatcgtc atgcccgtgg gcggccagag ctccttctac  240

agcgactggt atagccctgc ctgcggcaaa gccggatgcc agacctacaa gtgggagacc  300

tttctgacca gcgaactgcc ccagtggctg tccgccaata gggccgtcaa acctaccggc  360

tccgctgcca tcggactcag catggccgga agctccgcta tgatcctggc cgcctaccac  420

ccccagcaat ttatctacgc tggcagcctg tccgctctgc tggatcctag ccaaggcatg  480

ggccctagcc tcattggcct ggccatgggc gatgctggcg gctataaggc cgccgatatg  540

tggggcccta gctccgatcc tgcctgggag aggaatgacc ccacccagca gatccccaag  600

ctggtggcca acaacacaag gctctgggtg tactgcggca atggcacccc caacgaactg  660

ggcggagcca acattcccgc cgagttcctg gagaacttcg tcaggagcag caacctgaag  720

ttccaggacg cctacaatgc cgccggaggc cacaacgctg tgttcaactt ccctcccaac  780

ggcacccaca gctgggagta ttggggcgct cagctgaacg ccatgaaagg cgacctccag  840

agctccctgg gagctggacc cgggaccgag cagcagtgga acttcgccgg catcgaagct  900

gccgctagcg ccatccaagg caacgtgacc agcatccaca gcctgctgga cgagggcaag  960

cagagcctga ccaagctggc tgctgcttgg ggcggatccg gaagcgaagc ctaccagggc  1020

gtgcagcaga agtgggacgc cacagccacc gagctgaaca acgccctgca gaacctcgcc  1080

agaaccatca gcgaggccgg acaggctatg gccagcacag agggcaatgt gaccggcatg  1140

ttcgccttcg aaatcgccac caccagggac agggaaggcg ctaccatgat caccttcagg  1200

ctgaggctcc cctgcaggac catcctgagg gtgttcagca ggaaccccct ggtgaggggc  1260

accgacagac tggaagccgt gcaggacagc aggagccacg tgtatgccca ccaggctcag  1320

accaggcacc ctgctaccgc caccgtgatc gaccacgagg gcgtgatcga ctccaacacc  1380

accgccacca gcgctcctcc cagaaccaag atcacagtgc ccgccaggtg ggtggtgaac  1440

ggcatcgaga ggagcggcga ggtgaacgcc aagcctggaa ccaagagcgg cgacagggtg  1500

ggcatttggg tcgatagcgc cggccagctg gtggatgaac ctgctccccc tgccagagcc  1560

atcgccgata gggccatcct gatcagggtg aggaacgcca gctggcagca cgacatcgac  1620

agcctgttct gcacccaaag gcgatcgaca acagccaggg acatcatgaa cgccggcgtg  1680

acctgcgtgg gagagcatga aaccctcacc gccgccgccc aatacatgag ggagcacgac  1740

atcggcgccc tgcccatctg tggagacgac gacaggctgc acggcatgct gaccgacagg  1800

gacatcgtga tcaagggcct ggctgccggc ctcgatccta acaccgctac agccggcgag  1860

ctggccagag acagcatcta ctacgtggac gccaacgcca gcatccagga gatgctcaac  1920

gtgatggagg agcaccaggt gagaagggtg cctgtgatca gcgagcacag gctggtgggc  1980

atcgtgaccg aggccgatat cgctaggcac ctgcccgagc acgccatcgt gcagttcgtg  2040

aaggccatct gcagccccat ggctctggcc agcggcgcgc ccacccccgg actcctcacc  2100

acagctggag ctggcaggcc cagagacaga tgcgccagga tcgtgtgcac cgtgttcatc  2160

gagaccgccg tggtggctac catgttcgtg gccctgctgg gcctgagcac catcagcagc  2220

aaggccgacg acatcgactg ggacgccatc gcccagtgtg aatccggcgg aaactgggcc  2280

gccaataccg gcaatggcct gtacggcggc ctgcagatca gccaggctac ctgggactcc  2340

aacggaggag tgggaagccc tgccgctgct tcccctcagc agcagatcga ggtggccgac  2400

aacatcatga agacccaagg ccctggcgcc tggcctaagt gttccagctg tagccagggc  2460

gatgctcctc tgggcagcct gacccacatc ctgacctttc tcgccgccga gacaggcgga  2520

tgtagcggaa gcagggacga ctaatgatag                                   2550


<210> 61
<211> 816
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Ag85B-ESAT6-Rv1733c-Rv2626c-
      RpfD  (E. coli optimized)

<400> 61
Met Phe Ser Arg Pro Gly Leu Pro Val Glu Tyr Leu Gln Val Pro Ser 
1               5                   10                  15

Pro Ser Met Gly Arg Asp Ile Lys Val Gln Phe Gln Ser Gly Gly Asn 
            20                  25                  30

Asn Ser Pro Ala Val Tyr Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp 
        35                  40                  45

Tyr Asn Gly Trp Asp Ile Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln 
    50                  55                  60

Ser Gly Leu Ser Ile Val Met Pro Val Gly Gly Gln Ser Ser Phe Tyr 
65                  70                  75                  80

Ser Asp Trp Tyr Ser Pro Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr 
                85                  90                  95

Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala 
            100                 105                 110

Asn Arg Ala Val Lys Pro Thr Gly Ser Ala Ala Ile Gly Leu Ser Met 
        115                 120                 125

Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe 
    130                 135                 140

Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met 
145                 150                 155                 160

Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys 
                165                 170                 175

Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn 
            180                 185                 190

Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu 
        195                 200                 205

Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn 
    210                 215                 220

Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu Lys 
225                 230                 235                 240

Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn 
                245                 250                 255

Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu 
            260                 265                 270

Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly Ser 
        275                 280                 285

Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
    290                 295                 300

Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
305                 310                 315                 320

Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
                325                 330                 335

Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
            340                 345                 350

Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly 
        355                 360                 365

Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala Glu 
    370                 375                 380

Phe Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr 
385                 390                 395                 400

Phe Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg 
                405                 410                 415

Asn Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Pro Gly Val Gln 
            420                 425                 430

Asp Ser Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro 
        435                 440                 445

Ala Thr Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr 
    450                 455                 460

Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg 
465                 470                 475                 480

Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro 
                485                 490                 495

Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly 
            500                 505                 510

Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Ser 
        515                 520                 525

Arg Arg Ala Ile Leu Ile Arg Val Arg Asn Ala Ser Trp Gln His Asp 
    530                 535                 540

Ile Asp Ser Leu Phe Cys Thr Gln Arg Glu Leu Met Thr Thr Ala Arg 
545                 550                 555                 560

Asp Ile Met Asn Ala Gly Val Thr Cys Val Gly Glu His Glu Thr Leu 
                565                 570                 575

Thr Ala Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly Ala Leu Pro 
            580                 585                 590

Ile Cys Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr Asp Arg Asp 
        595                 600                 605

Ile Val Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn Thr Ala Thr 
    610                 615                 620

Ala Gly Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp Ala Asn Ala 
625                 630                 635                 640

Ser Ile Gln Glu Met Leu Asn Val Met Glu Glu His Gln Val Arg Arg 
                645                 650                 655

Val Pro Val Ile Ser Glu His Arg Leu Val Gly Ile Val Thr Glu Ala 
            660                 665                 670

Asp Ile Ala Arg His Leu Pro Glu His Ala Ile Val Gln Phe Val Lys 
        675                 680                 685

Ala Ile Cys Ser Pro Met Ala Leu Ala Ser Lys Leu Leu Leu Gly Leu 
    690                 695                 700

Ser Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp Asp Ala Ile Ala 
705                 710                 715                 720

Gln Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu 
                725                 730                 735

Tyr Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp Ser Asn Gly Gly 
            740                 745                 750

Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu Val Ala 
        755                 760                 765

Asp Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp Pro Lys Cys Ser 
    770                 775                 780

Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His Ile Leu 
785                 790                 795                 800

Thr Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly Ser Arg Asp Asp  
                805                 810                 815


<210> 62
<211> 847
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Ag85B-ESAT6-Rv1733c-Rv2626c-
      RpfD  (human optimized)

<400> 62
Met Phe Ser Arg Pro Gly Leu Pro Val Glu Tyr Leu Gln Val Pro Ser 
1               5                   10                  15

Pro Ser Met Gly Arg Asp Ile Lys Val Gln Phe Gln Ser Gly Gly Asn 
            20                  25                  30

Asn Ser Pro Ala Val Tyr Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp 
        35                  40                  45

Tyr Asn Gly Trp Asp Ile Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln 
    50                  55                  60

Ser Gly Leu Ser Ile Val Met Pro Val Gly Gly Gln Ser Ser Phe Tyr 
65                  70                  75                  80

Ser Asp Trp Tyr Ser Pro Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr 
                85                  90                  95

Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala 
            100                 105                 110

Asn Arg Ala Val Lys Pro Thr Gly Ser Ala Ala Ile Gly Leu Ser Met 
        115                 120                 125

Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe 
    130                 135                 140

Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met 
145                 150                 155                 160

Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys 
                165                 170                 175

Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn 
            180                 185                 190

Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu 
        195                 200                 205

Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn 
    210                 215                 220

Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu Lys 
225                 230                 235                 240

Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn 
                245                 250                 255

Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu 
            260                 265                 270

Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Pro Gly 
        275                 280                 285

Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala 
    290                 295                 300

Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys 
305                 310                 315                 320

Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu 
                325                 330                 335

Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu 
            340                 345                 350

Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln 
        355                 360                 365

Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala Phe Glu 
    370                 375                 380

Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe Arg 
385                 390                 395                 400

Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn Pro 
                405                 410                 415

Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Val Gln Asp Ser Arg Ser 
            420                 425                 430

His Val Tyr Ala His Gln Ala Gln Thr Arg His Pro Ala Thr Ala Thr 
        435                 440                 445

Val Ile Asp His Glu Gly Val Ile Asp Ser Asn Thr Thr Ala Thr Ser 
    450                 455                 460

Ala Pro Pro Arg Thr Lys Ile Thr Val Pro Ala Arg Trp Val Val Asn 
465                 470                 475                 480

Gly Ile Glu Arg Ser Gly Glu Val Asn Ala Lys Pro Gly Thr Lys Ser 
                485                 490                 495

Gly Asp Arg Val Gly Ile Trp Val Asp Ser Ala Gly Gln Leu Val Asp 
            500                 505                 510

Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala Asp Arg Ala Ile Leu Ile 
        515                 520                 525

Arg Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys 
    530                 535                 540

Thr Gln Arg Arg Ser Thr Thr Ala Arg Asp Ile Met Asn Ala Gly Val 
545                 550                 555                 560

Thr Cys Val Gly Glu His Glu Thr Leu Thr Ala Ala Ala Gln Tyr Met 
                565                 570                 575

Arg Glu His Asp Ile Gly Ala Leu Pro Ile Cys Gly Asp Asp Asp Arg 
            580                 585                 590

Leu His Gly Met Leu Thr Asp Arg Asp Ile Val Ile Lys Gly Leu Ala 
        595                 600                 605

Ala Gly Leu Asp Pro Asn Thr Ala Thr Ala Gly Glu Leu Ala Arg Asp 
    610                 615                 620

Ser Ile Tyr Tyr Val Asp Ala Asn Ala Ser Ile Gln Glu Met Leu Asn 
625                 630                 635                 640

Val Met Glu Glu His Gln Val Arg Arg Val Pro Val Ile Ser Glu His 
                645                 650                 655

Arg Leu Val Gly Ile Val Thr Glu Ala Asp Ile Ala Arg His Leu Pro 
            660                 665                 670

Glu His Ala Ile Val Gln Phe Val Lys Ala Ile Cys Ser Pro Met Ala 
        675                 680                 685

Leu Ala Ser Gly Ala Pro Thr Pro Gly Leu Leu Thr Thr Ala Gly Ala 
    690                 695                 700

Gly Arg Pro Arg Asp Arg Cys Ala Arg Ile Val Cys Thr Val Phe Ile 
705                 710                 715                 720

Glu Thr Ala Val Val Ala Thr Met Phe Val Ala Leu Leu Gly Leu Ser 
                725                 730                 735

Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp Asp Ala Ile Ala Gln 
            740                 745                 750

Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu Tyr 
        755                 760                 765

Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp Ser Asn Gly Gly Val 
    770                 775                 780

Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu Val Ala Asp 
785                 790                 795                 800

Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp Pro Lys Cys Ser Ser 
                805                 810                 815

Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His Ile Leu Thr 
            820                 825                 830

Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly Ser Arg Asp Asp
        835                 840                 845


<210> 63
<211> 2499
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium PPE51-Rv1733c-
      Rv2628c-RpfD fusion protein

<400> 63
atggattttg cgctgctgcc gccggaagtg aacagcgcgc gcatgtatac cggcccgggc  60

gcgggcagcc tgctggcggc ggcgggcggc tgggatagcc tggcggcgga actggcgacc  120

accgcggaag cgtatggcag cgtgctgagc ggcctggcgg cgctgcattg gcgcggcccg  180

gcggcggaaa gcatggcggt gaccgcggcg ccgtatattg gctggctgta taccaccgcg  240

gaaaaaaccc agcagaccgc gattcaggcg cgcgcggcgg cgctggcgtt tgaacaggcg  300

tatgcgatga ccctgccgcc gccggtggtg gcggcgaacc gcattcagct gctggcgctg  360

attgcgacca acttttttgg ccagaacacc gcggcgattg cggcgaccga agcgcagtat  420

gcggaaatgt gggcgcagga tgcggcggcg atgtatggct atgcgaccgc gagcgcggcg  480

gcggcgctgc tgaccccgtt tagcccgccg cgccagacca ccaacccggc gggcctgacc  540

gcgcaggcgg cggcggtgag ccaggcgacc gatccgctga gcctgctgat tgaaaccgtg  600

acccaggcgc tgcaggcgct gaccattccg agctttattc cggaagattt tacctttctg  660

gatgcgattt ttgcgggcta tgcgaccgtg ggcgtgaccc aggatgtgga aagctttgtg  720

gcgggcacca ttggcgcgga aagcaacctg ggcctgctga acgtgggcga tgaaaacccg  780

gcggaagtga ccccgggcga ttttggcatt ggcgaactgg tgagcgcgac cagcccgggc  840

ggcggcgtga gcgcgagcgg cgcgggcggc gcggcgagcg tgggcaacac cgtgctggcg  900

agcgtgggcc gcgcgaacag cattggccag ctgagcgtgc cgccgagctg ggcggcgccg  960

agcacccgcc cggtgagcgc gctgagcccg gcgggcctga ccaccctgcc gggcaccgat  1020

gtggcggaac atggcatgcc gggcgtgccg ggcgtgccgg tggcggcggg ccgcgcgagc  1080

ggcgtgctgc cgcgctatgg cgtgcgcctg accgtgatgg cgcatccgcc ggcggcgggc  1140

gaattcatga ttgcgactac ccgtgatcgt gagggcgcga ccatgatcac gttccgtctg  1200

cgtctgccgt gtcgcaccat tttgcgcgtg ttttcgcgta acccgctggt ccgcggtacc  1260

gaccgtctgg aggccgttgt catgctgctg gcggttaccg tgagcctgct gacgatccca  1320

ttcgcagcgg cagctggcac ggccgtccaa gacagccgta gccatgtgta tgctcaccag  1380

gctcaaaccc gtcacccggc tactgccact gttatcgatc acgaaggcgt gattgactcc  1440

aataccacgg caacctccgc accgcctcgc accaagatta cggttcctgc gcgttgggtg  1500

gtgaatggta ttgaacgcag cggcgaagtt aatgccaaac cgggtaccaa aagcggtgac  1560

cgtgtgggca tctgggtcga tagcgccggt cagctggtcg acgagccggc accgccagcg  1620

cgtgcgatcg ccgatgcggc gctggctgcc ctgggtctgt ggctgagcgt ggcagcggtc  1680

gccggtgcgt tgctggcgct gacgcgcgca attctgatcc gcgttcgcaa tgcgagctgg  1740

cagcacgata ttgatagcct gttttgcacc caacgtgagc tcatgtccac gcaacgaccg  1800

aggcactccg gtattcgggc tgttggcccc tacgcatggg ccggccgatg tggtcggata  1860

ggcaggtggg gggtgcacca ggaggcgatg atgaatctag cgatatggca cccgcgcaag  1920

gtgcaatccg ccaccatcta tcaggtgacc gatcgctcgc acgacgggcg cacagcacgg  1980

gtgcctggtg acgagatcac tagcaccgtg tccggttggt tgtcggagtt gggcacccaa  2040

agcccgttgg ccgatgagct tgcgcgtgcg gtgcggatcg gcgactggcc cgctgcgtac  2100

gcaatcggtg agcacctgtc cgttgagatt gccgttgcgg tcaagctttt gctgggcctg  2160

agcaccatta gcagcaaagc ggatgacatc gactgggatg cgattgcgca gtgtgagagc  2220

ggtggcaatt gggcagcgaa taccggcaat ggcctgtacg gcggtctgca gatctcccag  2280

gcgacgtggg acagcaatgg tggcgtcggc agcccggctg ccgcgtcccc acaacaacag  2340

atcgaggtgg cagataacat tatgaaaacg cagggtccgg gtgcttggcc aaaatgctcc  2400

agctgcagcc agggtgacgc accgctgggc agcctgaccc acattctgac gttcctggca  2460

gcggaaaccg gtggttgtag cggtagccgc gatgactga                         2499


<210> 64
<211> 834
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: PPE51-Rv1733c-Rv2628c-RpfD 

<400> 64
Met Asp Phe Ala Leu Leu Pro Pro Glu Val Asn Ser Ala Arg Met Tyr 
1               5                   10                  15

Thr Gly Pro Gly Ala Gly Ser Leu Leu Ala Ala Ala Gly Gly Trp Asp 
            20                  25                  30

Ser Leu Ala Ala Glu Leu Ala Thr Thr Ala Glu Ala Tyr Gly Ser Val 
        35                  40                  45

Leu Ser Gly Leu Ala Ala Leu His Trp Arg Gly Pro Ala Ala Glu Ser 
    50                  55                  60

Met Ala Val Thr Ala Ala Pro Tyr Ile Gly Trp Leu Tyr Thr Thr Ala 
65                  70                  75                  80

Glu Lys Thr Gln Gln Thr Ala Ile Gln Ala Arg Ala Ala Ala Leu Ala 
                85                  90                  95

Phe Glu Gln Ala Tyr Ala Met Thr Leu Pro Pro Pro Val Val Ala Ala 
            100                 105                 110

Asn Arg Ile Gln Leu Leu Ala Leu Ile Ala Thr Asn Phe Phe Gly Gln 
        115                 120                 125

Asn Thr Ala Ala Ile Ala Ala Thr Glu Ala Gln Tyr Ala Glu Met Trp 
    130                 135                 140

Ala Gln Asp Ala Ala Ala Met Tyr Gly Tyr Ala Thr Ala Ser Ala Ala 
145                 150                 155                 160

Ala Ala Leu Leu Thr Pro Phe Ser Pro Pro Arg Gln Thr Thr Asn Pro 
                165                 170                 175

Ala Gly Leu Thr Ala Gln Ala Ala Ala Val Ser Gln Ala Thr Asp Pro 
            180                 185                 190

Leu Ser Leu Leu Ile Glu Thr Val Thr Gln Ala Leu Gln Ala Leu Thr 
        195                 200                 205

Ile Pro Ser Phe Ile Pro Glu Asp Phe Thr Phe Leu Asp Ala Ile Phe 
    210                 215                 220

Ala Gly Tyr Ala Thr Val Gly Val Thr Gln Asp Val Glu Ser Phe Val 
225                 230                 235                 240

Ala Gly Thr Ile Gly Ala Glu Ser Asn Leu Gly Leu Leu Asn Val Gly 
                245                 250                 255

Asp Glu Asn Pro Ala Glu Val Thr Pro Gly Asp Phe Gly Ile Gly Glu 
            260                 265                 270

Leu Val Ser Ala Thr Ser Pro Gly Gly Gly Val Ser Ala Ser Gly Ala 
        275                 280                 285

Gly Gly Ala Ala Ser Val Gly Asn Thr Val Leu Ala Ser Val Gly Arg 
    290                 295                 300

Ala Asn Ser Ile Gly Gln Leu Ser Val Pro Pro Ser Trp Ala Ala Pro 
305                 310                 315                 320

Ser Thr Arg Pro Val Ser Ala Leu Ser Pro Ala Gly Leu Thr Thr Leu 
                325                 330                 335

Pro Gly Thr Asp Val Ala Glu His Gly Met Pro Gly Val Pro Gly Val 
            340                 345                 350

Pro Val Ala Ala Gly Arg Ala Ser Gly Val Leu Pro Arg Tyr Gly Val 
        355                 360                 365

Arg Leu Thr Val Met Ala His Pro Pro Ala Ala Gly Glu Phe Glu Phe 
    370                 375                 380

Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe 
385                 390                 395                 400

Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn 
                405                 410                 415

Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala Val Val Met Leu Leu 
            420                 425                 430

Ala Val Thr Val Ser Leu Leu Thr Ile Pro Phe Ala Ala Ala Ala Gly 
        435                 440                 445

Thr Ala Val Gln Asp Ser Arg Ser His Val Tyr Ala His Gln Ala Gln 
    450                 455                 460

Thr Arg His Pro Ala Thr Ala Thr Val Ile Asp His Glu Gly Val Ile 
465                 470                 475                 480

Asp Ser Asn Thr Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr 
                485                 490                 495

Val Pro Ala Arg Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val 
            500                 505                 510

Asn Ala Lys Pro Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val 
        515                 520                 525

Asp Ser Ala Gly Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala 
    530                 535                 540

Ile Ala Asp Ala Ala Leu Ala Ala Leu Gly Leu Trp Leu Ser Val Ala 
545                 550                 555                 560

Ala Val Ala Gly Ala Leu Leu Ala Leu Thr Arg Ala Ile Leu Ile Arg 
                565                 570                 575

Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys Thr 
            580                 585                 590

Gln Arg Glu Leu Met Ser Thr Gln Arg Pro Arg His Ser Gly Ile Arg 
        595                 600                 605

Ala Val Gly Pro Tyr Ala Trp Ala Gly Arg Cys Gly Arg Ile Gly Arg 
    610                 615                 620

Trp Gly Val His Gln Glu Ala Met Met Asn Leu Ala Ile Trp His Pro 
625                 630                 635                 640

Arg Lys Val Gln Ser Ala Thr Ile Tyr Gln Val Thr Asp Arg Ser His 
                645                 650                 655

Asp Gly Arg Thr Ala Arg Val Pro Gly Asp Glu Ile Thr Ser Thr Val 
            660                 665                 670

Ser Gly Trp Leu Ser Glu Leu Gly Thr Gln Ser Pro Leu Ala Asp Glu 
        675                 680                 685

Leu Ala Arg Ala Val Arg Ile Gly Asp Trp Pro Ala Ala Tyr Ala Ile 
    690                 695                 700

Gly Glu His Leu Ser Val Glu Ile Ala Val Ala Val Lys Leu Leu Leu 
705                 710                 715                 720

Gly Leu Ser Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp Asp Ala 
                725                 730                 735

Ile Ala Gln Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly Asn 
            740                 745                 750

Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp Ser Asn 
        755                 760                 765

Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile Glu 
    770                 775                 780

Val Ala Asp Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp Pro Lys 
785                 790                 795                 800

Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr His 
                805                 810                 815

Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly Ser Arg 
            820                 825                 830

Asp Asp 


<210> 65
<211> 3171
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium PPE51-Rv1733c-
      Rv2628c-RpfB fusion protein

<400> 65
atggattttg cgctgctgcc gccggaagtg aacagcgcgc gcatgtatac cggcccgggc  60

gcgggcagcc tgctggcggc ggcgggcggc tgggatagcc tggcggcgga actggcgacc  120

accgcggaag cgtatggcag cgtgctgagc ggcctggcgg cgctgcattg gcgcggcccg  180

gcggcggaaa gcatggcggt gaccgcggcg ccgtatattg gctggctgta taccaccgcg  240

gaaaaaaccc agcagaccgc gattcaggcg cgcgcggcgg cgctggcgtt tgaacaggcg  300

tatgcgatga ccctgccgcc gccggtggtg gcggcgaacc gcattcagct gctggcgctg  360

attgcgacca acttttttgg ccagaacacc gcggcgattg cggcgaccga agcgcagtat  420

gcggaaatgt gggcgcagga tgcggcggcg atgtatggct atgcgaccgc gagcgcggcg  480

gcggcgctgc tgaccccgtt tagcccgccg cgccagacca ccaacccggc gggcctgacc  540

gcgcaggcgg cggcggtgag ccaggcgacc gatccgctga gcctgctgat tgaaaccgtg  600

acccaggcgc tgcaggcgct gaccattccg agctttattc cggaagattt tacctttctg  660

gatgcgattt ttgcgggcta tgcgaccgtg ggcgtgaccc aggatgtgga aagctttgtg  720

gcgggcacca ttggcgcgga aagcaacctg ggcctgctga acgtgggcga tgaaaacccg  780

gcggaagtga ccccgggcga ttttggcatt ggcgaactgg tgagcgcgac cagcccgggc  840

ggcggcgtga gcgcgagcgg cgcgggcggc gcggcgagcg tgggcaacac cgtgctggcg  900

agcgtgggcc gcgcgaacag cattggccag ctgagcgtgc cgccgagctg ggcggcgccg  960

agcacccgcc cggtgagcgc gctgagcccg gcgggcctga ccaccctgcc gggcaccgat  1020

gtggcggaac atggcatgcc gggcgtgccg ggcgtgccgg tggcggcggg ccgcgcgagc  1080

ggcgtgctgc cgcgctatgg cgtgcgcctg accgtgatgg cgcatccgcc ggcggcgggc  1140

gaattcatga ttgcgactac ccgtgatcgt gagggcgcga ccatgatcac gttccgtctg  1200

cgtctgccgt gtcgcaccat tttgcgcgtg ttttcgcgta acccgctggt ccgcggtacc  1260

gaccgtctgg aggccgttgt catgctgctg gcggttaccg tgagcctgct gacgatccca  1320

ttcgcagcgg cagctggcac ggccgtccaa gacagccgta gccatgtgta tgctcaccag  1380

gctcaaaccc gtcacccggc tactgccact gttatcgatc acgaaggcgt gattgactcc  1440

aataccacgg caacctccgc accgcctcgc accaagatta cggttcctgc gcgttgggtg  1500

gtgaatggta ttgaacgcag cggcgaagtt aatgccaaac cgggtaccaa aagcggtgac  1560

cgtgtgggca tctgggtcga tagcgccggt cagctggtcg acgagccggc accgccagcg  1620

cgtgcgatcg ccgatgcggc gctggctgcc ctgggtctgt ggctgagcgt ggcagcggtc  1680

gccggtgcgt tgctggcgct gacgcgcgca attctgatcc gcgttcgcaa tgcgagctgg  1740

cagcacgata ttgatagcct gttttgcacc caacgtgagc tcatgtccac gcaacgaccg  1800

aggcactccg gtattcgggc tgttggcccc tacgcatggg ccggccgatg tggtcggata  1860

ggcaggtggg gggtgcacca ggaggcgatg atgaatctag cgatatggca cccgcgcaag  1920

gtgcaatccg ccaccatcta tcaggtgacc gatcgctcgc acgacgggcg cacagcacgg  1980

gtgcctggtg acgagatcac tagcaccgtg tccggttggt tgtcggagtt gggcacccaa  2040

agcccgttgg ccgatgagct tgcgcgtgcg gtgcggatcg gcgactggcc cgctgcgtac  2100

gcaatcggtg agcacctgtc cgttgagatt gccgttgcgg tcaagcttgc atgcaaaacg  2160

gtgacgttga ccgtcgacgg aaccgcgatg cgggtgacca cgatgaaatc gcgggtgatc  2220

gacatcgtcg aagagaacgg gttctcagtc gacgaccgcg acgacctgta tcccgcggcc  2280

ggcgtgcagg tccatgacgc cgacaccatc gtgctgcggc gtagccgtcc gctgcagatc  2340

tcgctggatg gtcacgacgc taagcaggtg tggacgaccg cgtcgacggt ggacgaggcg  2400

ctggcccaac tcgcgatgac cgacacggcg ccggccgcgg cttctcgcgc cagccgcgtc  2460

ccgctgtccg ggatggcgct accggtcgtc agcgccaaga cggtgcagct caacgacggc  2520

gggttggtgc gcacggtgca cttgccggcc cccaatgtcg cggggctgct gagtgcggcc  2580

ggcgtgccgc tgttgcaaag cgaccacgtg gtgcccgccg cgacggcccc gatcgtcgaa  2640

ggcatgcaga tccaggtgac ccgcaatcgg atcaagaagg tcaccgagcg gctgccgctg  2700

ccgccgaacg cgcgtcgtgt cgaggacccg gagatgaaca tgagccggga ggtcgtcgaa  2760

gacccggggg ttccggggac ccaggatgtg acgttcgcgg tagctgaggt caacggcgtc  2820

gagaccggcc gtttgcccgt cgccaacgtc gtggtgaccc cggcccacga agccgtggtg  2880

cgggtgggca ccaagcccgg taccgaggtg cccccggtga tcgacggaag catctgggac  2940

gcgatcgccg gctgtgaggc cggtggcaac tgggcgatca acaccggcaa cgggtattac  3000

ggtggtgtgc agtttgacca gggcacctgg gaggccaacg gcgggctgcg gtatgcaccc  3060

cgcgctgacc tcgccacccg cgaagagcag atcgccgttg ccgaggtgac ccgactgcgt  3120

caaggttggg gcgcctggcc ggtatgtgct gcacgagcgg gtgcgcgctg a           3171


<210> 66
<211> 1056
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: PPE51-Rv1733c-Rv2628c-RpfB 

<400> 66
Met Asp Phe Ala Leu Leu Pro Pro Glu Val Asn Ser Ala Arg Met Tyr 
1               5                   10                  15

Thr Gly Pro Gly Ala Gly Ser Leu Leu Ala Ala Ala Gly Gly Trp Asp 
            20                  25                  30

Ser Leu Ala Ala Glu Leu Ala Thr Thr Ala Glu Ala Tyr Gly Ser Val 
        35                  40                  45

Leu Ser Gly Leu Ala Ala Leu His Trp Arg Gly Pro Ala Ala Glu Ser 
    50                  55                  60

Met Ala Val Thr Ala Ala Pro Tyr Ile Gly Trp Leu Tyr Thr Thr Ala 
65                  70                  75                  80

Glu Lys Thr Gln Gln Thr Ala Ile Gln Ala Arg Ala Ala Ala Leu Ala 
                85                  90                  95

Phe Glu Gln Ala Tyr Ala Met Thr Leu Pro Pro Pro Val Val Ala Ala 
            100                 105                 110

Asn Arg Ile Gln Leu Leu Ala Leu Ile Ala Thr Asn Phe Phe Gly Gln 
        115                 120                 125

Asn Thr Ala Ala Ile Ala Ala Thr Glu Ala Gln Tyr Ala Glu Met Trp 
    130                 135                 140

Ala Gln Asp Ala Ala Ala Met Tyr Gly Tyr Ala Thr Ala Ser Ala Ala 
145                 150                 155                 160

Ala Ala Leu Leu Thr Pro Phe Ser Pro Pro Arg Gln Thr Thr Asn Pro 
                165                 170                 175

Ala Gly Leu Thr Ala Gln Ala Ala Ala Val Ser Gln Ala Thr Asp Pro 
            180                 185                 190

Leu Ser Leu Leu Ile Glu Thr Val Thr Gln Ala Leu Gln Ala Leu Thr 
        195                 200                 205

Ile Pro Ser Phe Ile Pro Glu Asp Phe Thr Phe Leu Asp Ala Ile Phe 
    210                 215                 220

Ala Gly Tyr Ala Thr Val Gly Val Thr Gln Asp Val Glu Ser Phe Val 
225                 230                 235                 240

Ala Gly Thr Ile Gly Ala Glu Ser Asn Leu Gly Leu Leu Asn Val Gly 
                245                 250                 255

Asp Glu Asn Pro Ala Glu Val Thr Pro Gly Asp Phe Gly Ile Gly Glu 
            260                 265                 270

Leu Val Ser Ala Thr Ser Pro Gly Gly Gly Val Ser Ala Ser Gly Ala 
        275                 280                 285

Gly Gly Ala Ala Ser Val Gly Asn Thr Val Leu Ala Ser Val Gly Arg 
    290                 295                 300

Ala Asn Ser Ile Gly Gln Leu Ser Val Pro Pro Ser Trp Ala Ala Pro 
305                 310                 315                 320

Ser Thr Arg Pro Val Ser Ala Leu Ser Pro Ala Gly Leu Thr Thr Leu 
                325                 330                 335

Pro Gly Thr Asp Val Ala Glu His Gly Met Pro Gly Val Pro Gly Val 
            340                 345                 350

Pro Val Ala Ala Gly Arg Ala Ser Gly Val Leu Pro Arg Tyr Gly Val 
        355                 360                 365

Arg Leu Thr Val Met Ala His Pro Pro Ala Ala Gly Glu Phe Met Ile 
    370                 375                 380

Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe Arg Leu 
385                 390                 395                 400

Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn Pro Leu 
                405                 410                 415

Val Arg Gly Thr Asp Arg Leu Glu Ala Val Val Met Leu Leu Ala Val 
            420                 425                 430

Thr Val Ser Leu Leu Thr Ile Pro Phe Ala Ala Ala Ala Gly Thr Ala 
        435                 440                 445

Val Gln Asp Ser Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg 
    450                 455                 460

His Pro Ala Thr Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser 
465                 470                 475                 480

Asn Thr Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro 
                485                 490                 495

Ala Arg Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala 
            500                 505                 510

Lys Pro Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser 
        515                 520                 525

Ala Gly Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala 
    530                 535                 540

Asp Ala Ala Leu Ala Ala Leu Gly Leu Trp Leu Ser Val Ala Ala Val 
545                 550                 555                 560

Ala Gly Ala Leu Leu Ala Leu Thr Arg Ala Ile Leu Ile Arg Val Arg 
                565                 570                 575

Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys Thr Gln Arg 
            580                 585                 590

Glu Leu Met Ser Thr Gln Arg Pro Arg His Ser Gly Ile Arg Ala Val 
        595                 600                 605

Gly Pro Tyr Ala Trp Ala Gly Arg Cys Gly Arg Ile Gly Arg Trp Gly 
    610                 615                 620

Val His Gln Glu Ala Met Met Asn Leu Ala Ile Trp His Pro Arg Lys 
625                 630                 635                 640

Val Gln Ser Ala Thr Ile Tyr Gln Val Thr Asp Arg Ser His Asp Gly 
                645                 650                 655

Arg Thr Ala Arg Val Pro Gly Asp Glu Ile Thr Ser Thr Val Ser Gly 
            660                 665                 670

Trp Leu Ser Glu Leu Gly Thr Gln Ser Pro Leu Ala Asp Glu Leu Ala 
        675                 680                 685

Arg Ala Val Arg Ile Gly Asp Trp Pro Ala Ala Tyr Ala Ile Gly Glu 
    690                 695                 700

His Leu Ser Val Glu Ile Ala Val Ala Val Lys Leu Ala Cys Lys Thr 
705                 710                 715                 720

Val Thr Leu Thr Val Asp Gly Thr Ala Met Arg Val Thr Thr Met Lys 
                725                 730                 735

Ser Arg Val Ile Asp Ile Val Glu Glu Asn Gly Phe Ser Val Asp Asp 
            740                 745                 750

Arg Asp Asp Leu Tyr Pro Ala Ala Gly Val Gln Val His Asp Ala Asp 
        755                 760                 765

Thr Ile Val Leu Arg Arg Ser Arg Pro Leu Gln Ile Ser Leu Asp Gly 
    770                 775                 780

His Asp Ala Lys Gln Val Trp Thr Thr Ala Ser Thr Val Asp Glu Ala 
785                 790                 795                 800

Leu Ala Gln Leu Ala Met Thr Asp Thr Ala Pro Ala Ala Ala Ser Arg 
                805                 810                 815

Ala Ser Arg Val Pro Leu Ser Gly Met Ala Leu Pro Val Val Ser Ala 
            820                 825                 830

Lys Thr Val Gln Leu Asn Asp Gly Gly Leu Val Arg Thr Val His Leu 
        835                 840                 845

Pro Ala Pro Asn Val Ala Gly Leu Leu Ser Ala Ala Gly Val Pro Leu 
    850                 855                 860

Leu Gln Ser Asp His Val Val Pro Ala Ala Thr Ala Pro Ile Val Glu 
865                 870                 875                 880

Gly Met Gln Ile Gln Val Thr Arg Asn Arg Ile Lys Lys Val Thr Glu 
                885                 890                 895

Arg Leu Pro Leu Pro Pro Asn Ala Arg Arg Val Glu Asp Pro Glu Met 
            900                 905                 910

Asn Met Ser Arg Glu Val Val Glu Asp Pro Gly Val Pro Gly Thr Gln 
        915                 920                 925

Asp Val Thr Phe Ala Val Ala Glu Val Asn Gly Val Glu Thr Gly Arg 
    930                 935                 940

Leu Pro Val Ala Asn Val Val Val Thr Pro Ala His Glu Ala Val Val 
945                 950                 955                 960

Arg Val Gly Thr Lys Pro Gly Thr Glu Val Pro Pro Val Ile Asp Gly 
                965                 970                 975

Ser Ile Trp Asp Ala Ile Ala Gly Cys Glu Ala Gly Gly Asn Trp Ala 
            980                 985                 990

Ile Asn Thr Gly Asn Gly Tyr Tyr Gly Gly Val Gln Phe Asp Gln Gly 
        995                 1000                1005

Thr Trp Glu Ala Asn Gly Gly Leu Arg Tyr Ala Pro Arg Ala Asp Leu 
    1010                1015                1020

Ala Thr Arg Glu Glu Gln Ile Ala Val Ala Glu Val Thr Arg Leu Arg 
1025                1030                1035                1040

Gln Gly Trp Gly Ala Trp Pro Val Cys Ala Ala Arg Ala Gly Ala Arg
                1045                1050                1055


<210> 67
<211> 2433
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3407-Rv1733c-
      Rv2626c-RpfB fusion protein

<400> 67
atgcgtgcga ctgtgggtct ggttgaggcg attggcattc gcgagctgcg ccaacatgcc  60

agccgttact tggctcgtgt cgaggcgggt gaagaactgg gcgtgacgaa taagggtcgt  120

ctggtcgccc gtctgattcc ggttcaggca gctgagcgtt ctcgcgaggc gctgattgaa  180

tccggcgtcc tgatcccggc tcgccgtccg caaaacctgc tggacgtgac ggcggagcca  240

gctcgtggtc gcaaacgcac gctgtctgat gtcctgaacg aaatgcgcga cgagcaggaa  300

ttcatgattg cgactacccg tgatcgtgag ggcgcgacca tgatcacgtt ccgtctgcgt  360

ctgccgtgtc gcaccatttt gcgcgtgttt tcgcgtaacc cgctggtccg cggtaccgac  420

cgtctggagg ccgttgtcat gctgctggcg gttaccgtga gcctgctgac gatcccattc  480

gcagcggcag ctggcacggc cgtccaagac agccgtagcc atgtgtatgc tcaccaggct  540

caaacccgtc acccggctac tgccactgtt atcgatcacg aaggcgtgat tgactccaat  600

accacggcaa cctccgcacc gcctcgcacc aagattacgg ttcctgcgcg ttgggtggtg  660

aatggtattg aacgcagcgg cgaagttaat gccaaaccgg gtaccaaaag cggtgaccgt  720

gtgggcatct gggtcgatag cgccggtcag ctggtcgacg agccggcacc gccagcgcgt  780

gcgatcgccg atgcggcgct ggctgccctg ggtctgtggc tgagcgtggc agcggtcgcc  840

ggtgcgttgc tggcgctgac gcgcgcaatt ctgatccgcg ttcgcaatgc gagctggcag  900

cacgatattg atagcctgtt ttgcacccaa cgtgagctca tgaccacggc gcgtgatatc  960

atgaatgcgg gtgtcacctg tgttggcgag cacgaaacgt tgaccgcagc agcacagtac  1020

atgcgcgaac atgatatcgg cgcattgccg atttgcggcg acgatgatcg tctgcacggt  1080

atgctgaccg accgcgatat cgttatcaag ggtctggccg caggcttgga cccgaacacc  1140

gcgaccgccg gtgaactggc acgtgacagc atctattacg tcgacgcgaa cgccagcatt  1200

caagagatgc tgaacgtgat ggaagagcat caggtgcgtc gtgtcccggt tatcagcgaa  1260

catcgtctgg ttggtatcgt taccgaagcc gacatcgcac gtcacctgcc ggagcacgcg  1320

attgttcagt tcgtgaaagc gatttgcagc ccgatggcgt tggcgtctcg tcaaaagggc  1380

gacacaaaat ttattctaaa tgcaaagctt gcatgcaaaa cggtgacgtt gaccgtcgac  1440

ggaaccgcga tgcgggtgac cacgatgaaa tcgcgggtga tcgacatcgt cgaagagaac  1500

gggttctcag tcgacgaccg cgacgacctg tatcccgcgg ccggcgtgca ggtccatgac  1560

gccgacacca tcgtgctgcg gcgtagccgt ccgctgcaga tctcgctgga tggtcacgac  1620

gctaagcagg tgtggacgac cgcgtcgacg gtggacgagg cgctggccca actcgcgatg  1680

accgacacgg cgccggccgc ggcttctcgc gccagccgcg tcccgctgtc cgggatggcg  1740

ctaccggtcg tcagcgccaa gacggtgcag ctcaacgacg gcgggttggt gcgcacggtg  1800

cacttgccgg cccccaatgt cgcggggctg ctgagtgcgg ccggcgtgcc gctgttgcaa  1860

agcgaccacg tggtgcccgc cgcgacggcc ccgatcgtcg aaggcatgca gatccaggtg  1920

acccgcaatc ggatcaagaa ggtcaccgag cggctgccgc tgccgccgaa cgcgcgtcgt  1980

gtcgaggacc cggagatgaa catgagccgg gaggtcgtcg aagacccggg ggttccgggg  2040

acccaggatg tgacgttcgc ggtagctgag gtcaacggcg tcgagaccgg ccgtttgccc  2100

gtcgccaacg tcgtggtgac cccggcccac gaagccgtgg tgcgggtggg caccaagccc  2160

ggtaccgagg tgcccccggt gatcgacgga agcatctggg acgcgatcgc cggctgtgag  2220

gccggtggca actgggcgat caacaccggc aacgggtatt acggtggtgt gcagtttgac  2280

cagggcacct gggaggccaa cggcgggctg cggtatgcac cccgcgctga cctcgccacc  2340

cgcgaagagc agatcgccgt tgccgaggtg acccgactgc gtcaaggttg gggcgcctgg  2400

ccggtatgtg ctgcacgagc gggtgcgcgc tga                               2433


<210> 68
<211> 810
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv3407-Rv1733c-Rv2626c-RpfB 

<400> 68
Met Arg Ala Thr Val Gly Leu Val Glu Ala Ile Gly Ile Arg Glu Leu 
1               5                   10                  15

Arg Gln His Ala Ser Arg Tyr Leu Ala Arg Val Glu Ala Gly Glu Glu 
            20                  25                  30

Leu Gly Val Thr Asn Lys Gly Arg Leu Val Ala Arg Leu Ile Pro Val 
        35                  40                  45

Gln Ala Ala Glu Arg Ser Arg Glu Ala Leu Ile Glu Ser Gly Val Leu 
    50                  55                  60

Ile Pro Ala Arg Arg Pro Gln Asn Leu Leu Asp Val Thr Ala Glu Pro 
65                  70                  75                  80

Ala Arg Gly Arg Lys Arg Thr Leu Ser Asp Val Leu Asn Glu Met Arg 
                85                  90                  95

Asp Glu Gln Glu Phe Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala 
            100                 105                 110

Thr Met Ile Thr Phe Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg 
        115                 120                 125

Val Phe Ser Arg Asn Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala 
    130                 135                 140

Val Val Met Leu Leu Ala Val Thr Val Ser Leu Leu Thr Ile Pro Phe
145                 150                 155                 160

Ala Ala Ala Ala Gly Thr Ala Val Gln Asp Ser Arg Ser His Val Tyr 
                165                 170                 175

Ala His Gln Ala Gln Thr Arg His Pro Ala Thr Ala Thr Val Ile Asp 
            180                 185                 190

His Glu Gly Val Ile Asp Ser Asn Thr Thr Ala Thr Ser Ala Pro Pro 
        195                 200                 205

Arg Thr Lys Ile Thr Val Pro Ala Arg Trp Val Val Asn Gly Ile Glu 
    210                 215                 220

Arg Ser Gly Glu Val Asn Ala Lys Pro Gly Thr Lys Ser Gly Asp Arg 
225                 230                 235                 240

Val Gly Ile Trp Val Asp Ser Ala Gly Gln Leu Val Asp Glu Pro Ala 
                245                 250                 255

Pro Pro Ala Arg Ala Ile Ala Asp Ala Ala Leu Ala Ala Leu Gly Leu 
            260                 265                 270

Trp Leu Ser Val Ala Ala Val Ala Gly Ala Leu Leu Ala Leu Thr Arg 
        275                 280                 285

Ala Ile Leu Ile Arg Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp 
    290                 295                 300

Ser Leu Phe Cys Thr Gln Arg Glu Leu Met Thr Thr Ala Arg Asp Ile 
305                 310                 315                 320

Met Asn Ala Gly Val Thr Cys Val Gly Glu His Glu Thr Leu Thr Ala 
                325                 330                 335

Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly Ala Leu Pro Ile Cys 
            340                 345                 350

Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr Asp Arg Asp Ile Val 
        355                 360                 365

Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn Thr Ala Thr Ala Gly 
    370                 375                 380

Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp Ala Asn Ala Ser Ile 
385                 390                 395                 400

Gln Glu Met Leu Asn Val Met Glu Glu His Gln Val Arg Arg Val Pro 
                405                 410                 415

Val Ile Ser Glu His Arg Leu Val Gly Ile Val Thr Glu Ala Asp Ile 
            420                 425                 430

Ala Arg His Leu Pro Glu His Ala Ile Val Gln Phe Val Lys Ala Ile 
        435                 440                 445

Cys Ser Pro Met Ala Leu Ala Ser Arg Gln Lys Gly Asp Thr Lys Phe 
    450                 455                 460

Ile Leu Asn Ala Lys Leu Ala Cys Lys Thr Val Thr Leu Thr Val Asp 
465                 470                 475                 480

Gly Thr Ala Met Arg Val Thr Thr Met Lys Ser Arg Val Ile Asp Ile 
                485                 490                 495

Val Glu Glu Asn Gly Phe Ser Val Asp Asp Arg Asp Asp Leu Tyr Pro 
            500                 505                 510

Ala Ala Gly Val Gln Val His Asp Ala Asp Thr Ile Val Leu Arg Arg 
        515                 520                 525

Ser Arg Pro Leu Gln Ile Ser Leu Asp Gly His Asp Ala Lys Gln Val 
    530                 535                 540

Trp Thr Thr Ala Ser Thr Val Asp Glu Ala Leu Ala Gln Leu Ala Met 
545                 550                 555                 560

Thr Asp Thr Ala Pro Ala Ala Ala Ser Arg Ala Ser Arg Val Pro Leu 
                565                 570                 575

Ser Gly Met Ala Leu Pro Val Val Ser Ala Lys Thr Val Gln Leu Asn 
            580                 585                 590

Asp Gly Gly Leu Val Arg Thr Val His Leu Pro Ala Pro Asn Val Ala 
        595                 600                 605

Gly Leu Leu Ser Ala Ala Gly Val Pro Leu Leu Gln Ser Asp His Val 
    610                 615                 620

Val Pro Ala Ala Thr Ala Pro Ile Val Glu Gly Met Gln Ile Gln Val 
625                 630                 635                 640

Thr Arg Asn Arg Ile Lys Lys Val Thr Glu Arg Leu Pro Leu Pro Pro 
                645                 650                 655

Asn Ala Arg Arg Val Glu Asp Pro Glu Met Asn Met Ser Arg Glu Val 
            660                 665                 670

Val Glu Asp Pro Gly Val Pro Gly Thr Gln Asp Val Thr Phe Ala Val 
        675                 680                 685

Ala Glu Val Asn Gly Val Glu Thr Gly Arg Leu Pro Val Ala Asn Val 
    690                 695                 700

Val Val Thr Pro Ala His Glu Ala Val Val Arg Val Gly Thr Lys Pro 
705                 710                 715                 720

Gly Thr Glu Val Pro Pro Val Ile Asp Gly Ser Ile Trp Asp Ala Ile 
                725                 730                 735

Ala Gly Cys Glu Ala Gly Gly Asn Trp Ala Ile Asn Thr Gly Asn Gly 
            740                 745                 750

Tyr Tyr Gly Gly Val Gln Phe Asp Gln Gly Thr Trp Glu Ala Asn Gly 
        755                 760                 765

Gly Leu Arg Tyr Ala Pro Arg Ala Asp Leu Ala Thr Arg Glu Glu Gln 
    770                 775                 780

Ile Ala Val Ala Glu Val Thr Arg Leu Arg Gln Gly Trp Gly Ala Trp 
785                 790                 795                 800

Pro Val Cys Ala Ala Arg Ala Gly Ala Arg 
                805                 810


<210> 69
<211> 1761
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium Rv3407-Rv1733c-
      Rv2626c-RpfD fusion protein

<400> 69
atgcgtgcga ctgtgggtct ggttgaggcg attggcattc gcgagctgcg ccaacatgcc  60

agccgttact tggctcgtgt cgaggcgggt gaagaactgg gcgtgacgaa taagggtcgt  120

ctggtcgccc gtctgattcc ggttcaggca gctgagcgtt ctcgcgaggc gctgattgaa  180

tccggcgtcc tgatcccggc tcgccgtccg caaaacctgc tggacgtgac ggcggagcca  240

gctcgtggtc gcaaacgcac gctgtctgat gtcctgaacg aaatgcgcga cgagcaggaa  300

ttcatgattg cgactacccg tgatcgtgag ggcgcgacca tgatcacgtt ccgtctgcgt  360

ctgccgtgtc gcaccatttt gcgcgtgttt tcgcgtaacc cgctggtccg cggtaccgac  420

cgtctggagg ccgttgtcat gctgctggcg gttaccgtga gcctgctgac gatcccattc  480

gcagcggcag ctggcacggc cgtccaagac agccgtagcc atgtgtatgc tcaccaggct  540

caaacccgtc acccggctac tgccactgtt atcgatcacg aaggcgtgat tgactccaat  600

accacggcaa cctccgcacc gcctcgcacc aagattacgg ttcctgcgcg ttgggtggtg  660

aatggtattg aacgcagcgg cgaagttaat gccaaaccgg gtaccaaaag cggtgaccgt  720

gtgggcatct gggtcgatag cgccggtcag ctggtcgacg agccggcacc gccagcgcgt  780

gcgatcgccg atgcggcgct ggctgccctg ggtctgtggc tgagcgtggc agcggtcgcc  840

ggtgcgttgc tggcgctgac gcgcgcaatt ctgatccgcg ttcgcaatgc gagctggcag  900

cacgatattg atagcctgtt ttgcacccaa cgtgagctca tgaccacggc gcgtgatatc  960

atgaatgcgg gtgtcacctg tgttggcgag cacgaaacgt tgaccgcagc agcacagtac  1020

atgcgcgaac atgatatcgg cgcattgccg atttgcggcg acgatgatcg tctgcacggt  1080

atgctgaccg accgcgatat cgttatcaag ggtctggccg caggcttgga cccgaacacc  1140

gcgaccgccg gtgaactggc acgtgacagc atctattacg tcgacgcgaa cgccagcatt  1200

caagagatgc tgaacgtgat ggaagagcat caggtgcgtc gtgtcccggt tatcagcgaa  1260

catcgtctgg ttggtatcgt taccgaagcc gacatcgcac gtcacctgcc ggagcacgcg  1320

attgttcagt tcgtgaaagc gatttgcagc ccgatggcgt tggcgtctcg tcaaaagggc  1380

gacacaaaat ttattctaaa tgcaaagctt ttgctgggcc tgagcaccat tagcagcaaa  1440

gcggatgaca tcgactggga tgcgattgcg cagtgtgaga gcggtggcaa ttgggcagcg  1500

aataccggca atggcctgta cggcggtctg cagatctccc aggcgacgtg ggacagcaat  1560

ggtggcgtcg gcagcccggc tgccgcgtcc ccacaacaac agatcgaggt ggcagataac  1620

attatgaaaa cgcagggtcc gggtgcttgg ccaaaatgct ccagctgcag ccagggtgac  1680

gcaccgctgg gcagcctgac ccacattctg acgttcctgg cagcggaaac cggtggttgt  1740

agcggtagcc gcgatgactg a                                            1761


<210> 70
<211> 586
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv3407-Rv1733c-Rv2626c-RpfD 

<400> 70
Met Arg Ala Thr Val Gly Leu Val Glu Ala Ile Gly Ile Arg Glu Leu 
1               5                   10                  15

Arg Gln His Ala Ser Arg Tyr Leu Ala Arg Val Glu Ala Gly Glu Glu 
            20                  25                  30

Leu Gly Val Thr Asn Lys Gly Arg Leu Val Ala Arg Leu Ile Pro Val 
        35                  40                  45

Gln Ala Ala Glu Arg Ser Arg Glu Ala Leu Ile Glu Ser Gly Val Leu 
    50                  55                  60

Ile Pro Ala Arg Arg Pro Gln Asn Leu Leu Asp Val Thr Ala Glu Pro 
65                  70                  75                  80

Ala Arg Gly Arg Lys Arg Thr Leu Ser Asp Val Leu Asn Glu Met Arg 
                85                  90                  95

Asp Glu Gln Glu Phe Met Ile Ala Thr Thr Arg Asp Arg Glu Gly Ala 
            100                 105                 110

Thr Met Ile Thr Phe Arg Leu Arg Leu Pro Cys Arg Thr Ile Leu Arg 
        115                 120                 125

Val Phe Ser Arg Asn Pro Leu Val Arg Gly Thr Asp Arg Leu Glu Ala 
    130                 135                 140

Val Val Met Leu Leu Ala Val Thr Val Ser Leu Leu Thr Ile Pro Phe 
145                 150                 155                 160

Ala Ala Ala Ala Gly Thr Ala Val Gln Asp Ser Arg Ser His Val Tyr 
                165                 170                 175

Ala His Gln Ala Gln Thr Arg His Pro Ala Thr Ala Thr Val Ile Asp 
            180                 185                 190

His Glu Gly Val Ile Asp Ser Asn Thr Thr Ala Thr Ser Ala Pro Pro 
        195                 200                 205

Arg Thr Lys Ile Thr Val Pro Ala Arg Trp Val Val Asn Gly Ile Glu 
    210                 215                 220

Arg Ser Gly Glu Val Asn Ala Lys Pro Gly Thr Lys Ser Gly Asp Arg 
225                 230                 235                 240

Val Gly Ile Trp Val Asp Ser Ala Gly Gln Leu Val Asp Glu Pro Ala 
                245                 250                 255

Pro Pro Ala Arg Ala Ile Ala Asp Ala Ala Leu Ala Ala Leu Gly Leu 
            260                 265                 270

Trp Leu Ser Val Ala Ala Val Ala Gly Ala Leu Leu Ala Leu Thr Arg 
        275                 280                 285

Ala Ile Leu Ile Arg Val Arg Asn Ala Ser Trp Gln His Asp Ile Asp 
    290                 295                 300

Ser Leu Phe Cys Thr Gln Arg Glu Leu Met Thr Thr Ala Arg Asp Ile 
305                 310                 315                 320

Met Asn Ala Gly Val Thr Cys Val Gly Glu His Glu Thr Leu Thr Ala 
                325                 330                 335

Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly Ala Leu Pro Ile Cys 
            340                 345                 350

Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr Asp Arg Asp Ile Val 
        355                 360                 365

Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn Thr Ala Thr Ala Gly 
    370                 375                 380

Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp Ala Asn Ala Ser Ile 
385                 390                 395                 400

Gln Glu Met Leu Asn Val Met Glu Glu His Gln Val Arg Arg Val Pro 
                405                 410                 415

Val Ile Ser Glu His Arg Leu Val Gly Ile Val Thr Glu Ala Asp Ile 
            420                 425                 430

Ala Arg His Leu Pro Glu His Ala Ile Val Gln Phe Val Lys Ala Ile 
        435                 440                 445

Cys Ser Pro Met Ala Leu Ala Ser Arg Gln Lys Gly Asp Thr Lys Phe 
    450                 455                 460

Ile Leu Asn Ala Lys Leu Leu Leu Gly Leu Ser Thr Ile Ser Ser Lys 
465                 470                 475                 480

Ala Asp Asp Ile Asp Trp Asp Ala Ile Ala Gln Cys Glu Ser Gly Gly 
                485                 490                 495

Asn Trp Ala Ala Asn Thr Gly Asn Gly Leu Tyr Gly Gly Leu Gln Ile 
            500                 505                 510

Ser Gln Ala Thr Trp Asp Ser Asn Gly Gly Val Gly Ser Pro Ala Ala 
        515                 520                 525

Ala Ser Pro Gln Gln Gln Ile Glu Val Ala Asp Asn Ile Met Lys Thr 
    530                 535                 540

Gln Gly Pro Gly Ala Trp Pro Lys Cys Ser Ser Cys Ser Gln Gly Asp 
545                 550                 555                 560

Ala Pro Leu Gly Ser Leu Thr His Ile Leu Thr Phe Leu Ala Ala Glu 
                565                 570                 575

Thr Gly Gly Cys Ser Gly Ser Arg Asp Asp 
            580                 585


<210> 71
<211> 2895
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium PPE51-Rv1733c-
      Rv2626c-RpfD fusion protein

<400> 71
atggattttg cgctgctgcc gccggaagtg aacagcgcgc gcatgtatac cggcccgggc  60

gcgggcagcc tgctggcggc ggcgggcggc tgggatagcc tggcggcgga actggcgacc  120

accgcggaag cgtatggcag cgtgctgagc ggcctggcgg cgctgcattg gcgcggcccg  180

gcggcggaaa gcatggcggt gaccgcggcg ccgtatattg gctggctgta taccaccgcg  240

gaaaaaaccc agcagaccgc gattcaggcg cgcgcggcgg cgctggcgtt tgaacaggcg  300

tatgcgatga ccctgccgcc gccggtggtg gcggcgaacc gcattcagct gctggcgctg  360

attgcgacca acttttttgg ccagaacacc gcggcgattg cggcgaccga agcgcagtat  420

gcggaaatgt gggcgcagga tgcggcggcg atgtatggct atgcgaccgc gagcgcggcg  480

gcggcgctgc tgaccccgtt tagcccgccg cgccagacca ccaacccggc gggcctgacc  540

gcgcaggcgg cggcggtgag ccaggcgacc gatccgctga gcctgctgat tgaaaccgtg  600

acccaggcgc tgcaggcgct gaccattccg agctttattc cggaagattt tacctttctg  660

gatgcgattt ttgcgggcta tgcgaccgtg ggcgtgaccc aggatgtgga aagctttgtg  720

gcgggcacca ttggcgcgga aagcaacctg ggcctgctga acgtgggcga tgaaaacccg  780

gcggaagtga ccccgggcga ttttggcatt ggcgaactgg tgagcgcgac cagcccgggc  840

ggcggcgtga gcgcgagcgg cgcgggcggc gcggcgagcg tgggcaacac cgtgctggcg  900

agcgtgggcc gcgcgaacag cattggccag ctgagcgtgc cgccgagctg ggcggcgccg  960

agcacccgcc cggtgagcgc gctgagcccg gcgggcctga ccaccctgcc gggcaccgat  1020

gtggcggaac atggcatgcc gggcgtgccg ggcgtgccgg tggcggcggg ccgcgcgagc  1080

ggcgtgctgc cgcgctatgg cgtgcgcctg accgtgatgg cgcatccgcc ggcggcgggc  1140

gaatttatga cagagcagca gtggaatttc gcgggtatcg aggccgcggc aagcgcaatc  1200

cagggaaatg tcacgtccat tcattccctc cttgacgagg ggaagcagtc cctgaccaag  1260

ctcgcagcgg cctggggcgg tagcggttcg gaggcgtacc agggtgtcca gcaaaaatgg  1320

gacgccacgg ctaccgagct gaacaacgcg ctgcagaacc tggcgcggac gatcagcgaa  1380

gccggtcagg caatggcttc gaccgaaggc aacgtcactg ggatgttcgc agaattcatg  1440

attgcgacta cccgtgatcg tgagggcgcg accatgatca cgttccgtct gcgtctgccg  1500

tgtcgcacca ttttgcgcgt gttttcgcgt aacccgctgg tccgcggtac cgaccgtctg  1560

gaggccgttg tcatgctgct ggcggttacc gtgagcctgc tgacgatccc attcgcagcg  1620

gcagctggca cggccgtcca agacagccgt agccatgtgt atgctcacca ggctcaaacc  1680

cgtcacccgg ctactgccac tgttatcgat cacgaaggcg tgattgactc caataccacg  1740

gcaacctccg caccgcctcg caccaagatt acggttcctg cgcgttgggt ggtgaatggt  1800

attgaacgca gcggcgaagt taatgccaaa ccgggtacca aaagcggtga ccgtgtgggc  1860

atctgggtcg atagcgccgg tcagctggtc gacgagccgg caccgccagc gcgtgcgatc  1920

gccgatgcgg cgctggctgc cctgggtctg tggctgagcg tggcagcggt cgccggtgcg  1980

ttgctggcgc tgacgcgcgc aattctgatc cgcgttcgca atgcgagctg gcagcacgat  2040

attgatagcc tgttttgcac ccaacgtgag ctcatgacca cggcgcgtga tatcatgaat  2100

gcgggtgtca cctgtgttgg cgagcacgaa acgttgaccg cagcagcaca gtacatgcgc  2160

gaacatgata tcggcgcatt gccgatttgc ggcgacgatg atcgtctgca cggtatgctg  2220

accgaccgcg atatcgttat caagggtctg gccgcaggct tggacccgaa caccgcgacc  2280

gccggtgaac tggcacgtga cagcatctat tacgtcgacg cgaacgccag cattcaagag  2340

atgctgaacg tgatggaaga gcatcaggtg cgtcgtgtcc cggttatcag cgaacatcgt  2400

ctggttggta tcgttaccga agccgacatc gcacgtcacc tgccggagca cgcgattgtt  2460

cagttcgtga aagcgatttg cagcccgatg gcgttggcgt ctcgtcaaaa gggcgacaca  2520

aaatttattc taaatgcaaa gcttttgctg ggcctgagca ccattagcag caaagcggat  2580

gacatcgact gggatgcgat tgcgcagtgt gagagcggtg gcaattgggc agcgaatacc  2640

ggcaatggcc tgtacggcgg tctgcagatc tcccaggcga cgtgggacag caatggtggc  2700

gtcggcagcc cggctgccgc gtccccacaa caacagatcg aggtggcaga taacattatg  2760

aaaacgcagg gtccgggtgc ttggccaaaa tgctccagct gcagccaggg tgacgcaccg  2820

ctgggcagcc tgacccacat tctgacgttc ctggcagcgg aaaccggtgg ttgtagcggt  2880

agccgcgatg actga                                                   2895


<210> 72
<211> 870
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: PPE51-Rv1733c-Rv2626c-RpfD 

<400> 72
Met Asp Phe Ala Leu Leu Pro Pro Glu Val Asn Ser Ala Arg Met Tyr 
1               5                   10                  15

Thr Gly Pro Gly Ala Gly Ser Leu Leu Ala Ala Ala Gly Gly Trp Asp 
            20                  25                  30

Ser Leu Ala Ala Glu Leu Ala Thr Thr Ala Glu Ala Tyr Gly Ser Val 
        35                  40                  45

Leu Ser Gly Leu Ala Ala Leu His Trp Arg Gly Pro Ala Ala Glu Ser 
    50                  55                  60

Met Ala Val Thr Ala Ala Pro Tyr Ile Gly Trp Leu Tyr Thr Thr Ala 
65                  70                  75                  80

Glu Lys Thr Gln Gln Thr Ala Ile Gln Ala Arg Ala Ala Ala Leu Ala 
                85                  90                  95

Phe Glu Gln Ala Tyr Ala Met Thr Leu Pro Pro Pro Val Val Ala Ala 
            100                 105                 110

Asn Arg Ile Gln Leu Leu Ala Leu Ile Ala Thr Asn Phe Phe Gly Gln 
        115                 120                 125

Asn Thr Ala Ala Ile Ala Ala Thr Glu Ala Gln Tyr Ala Glu Met Trp 
    130                 135                 140

Ala Gln Asp Ala Ala Ala Met Tyr Gly Tyr Ala Thr Ala Ser Ala Ala 
145                 150                 155                 160

Ala Ala Leu Leu Thr Pro Phe Ser Pro Pro Arg Gln Thr Thr Asn Pro 
                165                 170                 175

Ala Gly Leu Thr Ala Gln Ala Ala Ala Val Ser Gln Ala Thr Asp Pro 
            180                 185                 190

Leu Ser Leu Leu Ile Glu Thr Val Thr Gln Ala Leu Gln Ala Leu Thr 
        195                 200                 205

Ile Pro Ser Phe Ile Pro Glu Asp Phe Thr Phe Leu Asp Ala Ile Phe 
    210                 215                 220

Ala Gly Tyr Ala Thr Val Gly Val Thr Gln Asp Val Glu Ser Phe Val 
225                 230                 235                 240

Ala Gly Thr Ile Gly Ala Glu Ser Asn Leu Gly Leu Leu Asn Val Gly 
                245                 250                 255

Asp Glu Asn Pro Ala Glu Val Thr Pro Gly Asp Phe Gly Ile Gly Glu 
            260                 265                 270

Leu Val Ser Ala Thr Ser Pro Gly Gly Gly Val Ser Ala Ser Gly Ala 
        275                 280                 285

Gly Gly Ala Ala Ser Val Gly Asn Thr Val Leu Ala Ser Val Gly Arg 
    290                 295                 300

Ala Asn Ser Ile Gly Gln Leu Ser Val Pro Pro Ser Trp Ala Ala Pro 
305                 310                 315                 320

Ser Thr Arg Pro Val Ser Ala Leu Ser Pro Ala Gly Leu Thr Thr Leu 
                325                 330                 335

Pro Gly Thr Asp Val Ala Glu His Gly Met Pro Gly Val Pro Gly Val 
            340                 345                 350

Pro Val Ala Ala Gly Arg Ala Ser Gly Val Leu Pro Arg Tyr Gly Val 
        355                 360                 365

Arg Leu Thr Val Met Ala His Pro Pro Ala Ala Gly Glu Phe Met Ile 
    370                 375                 380

Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe Arg Leu 
385                 390                 395                 400

Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn Pro Leu 
                405                 410                 415

Val Arg Gly Thr Asp Arg Leu Glu Ala Val Val Met Leu Leu Ala Val 
            420                 425                 430

Thr Val Ser Leu Leu Thr Ile Pro Phe Ala Ala Ala Ala Gly Thr Ala 
        435                 440                 445

Val Gln Asp Ser Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg 
    450                 455                 460

His Pro Ala Thr Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser 
465                 470                 475                 480

Asn Thr Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro 
                485                 490                 495

Ala Arg Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala 
            500                 505                 510

Lys Pro Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser 
        515                 520                 525

Ala Gly Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala 
    530                 535                 540

Asp Ala Ala Leu Ala Ala Leu Gly Leu Trp Leu Ser Val Ala Ala Val 
545                 550                 555                 560

Ala Gly Ala Leu Leu Ala Leu Thr Arg Ala Ile Leu Ile Arg Val Arg 
                565                 570                 575

Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys Thr Gln Arg 
            580                 585                 590

Glu Leu Met Thr Thr Ala Arg Asp Ile Met Asn Ala Gly Val Thr Cys 
        595                 600                 605

Val Gly Glu His Glu Thr Leu Thr Ala Ala Ala Gln Tyr Met Arg Glu 
    610                 615                 620

His Asp Ile Gly Ala Leu Pro Ile Cys Gly Asp Asp Asp Arg Leu His 
625                 630                 635                 640

Gly Met Leu Thr Asp Arg Asp Ile Val Ile Lys Gly Leu Ala Ala Gly 
                645                 650                 655

Leu Asp Pro Asn Thr Ala Thr Ala Gly Glu Leu Ala Arg Asp Ser Ile 
            660                 665                 670

Tyr Tyr Val Asp Ala Asn Ala Ser Ile Gln Glu Met Leu Asn Val Met 
        675                 680                 685

Glu Glu His Gln Val Arg Arg Val Pro Val Ile Ser Glu His Arg Leu 
    690                 695                 700

Val Gly Ile Val Thr Glu Ala Asp Ile Ala Arg His Leu Pro Glu His 
705                 710                 715                 720

Ala Ile Val Gln Phe Val Lys Ala Ile Cys Ser Pro Met Ala Leu Ala 
                725                 730                 735

Ser Arg Gln Lys Gly Asp Thr Lys Phe Ile Leu Asn Ala Lys Leu Leu 
            740                 745                 750

Leu Gly Leu Ser Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp Asp 
        755                 760                 765

Ala Ile Ala Gln Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly 
    770                 775                 780

Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp Ser 
785                 790                 795                 800

Asn Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile 
                805                 810                 815

Glu Val Ala Asp Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp Pro 
            820                 825                 830

Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr 
        835                 840                 845

His Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly Ser 
    850                 855                 860

Arg Asp Asp Lys Met Lys
865                 870


<210> 73
<211> 3567
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mycobacterium PPE51-Rv1733c-
      Rv2626c-RpfB fusion protein

<400> 73
atggattttg cgctgctgcc gccggaagtg aacagcgcgc gcatgtatac cggcccgggc  60

gcgggcagcc tgctggcggc ggcgggcggc tgggatagcc tggcggcgga actggcgacc  120

accgcggaag cgtatggcag cgtgctgagc ggcctggcgg cgctgcattg gcgcggcccg  180

gcggcggaaa gcatggcggt gaccgcggcg ccgtatattg gctggctgta taccaccgcg  240

gaaaaaaccc agcagaccgc gattcaggcg cgcgcggcgg cgctggcgtt tgaacaggcg  300

tatgcgatga ccctgccgcc gccggtggtg gcggcgaacc gcattcagct gctggcgctg  360

attgcgacca acttttttgg ccagaacacc gcggcgattg cggcgaccga agcgcagtat  420

gcggaaatgt gggcgcagga tgcggcggcg atgtatggct atgcgaccgc gagcgcggcg  480

gcggcgctgc tgaccccgtt tagcccgccg cgccagacca ccaacccggc gggcctgacc  540

gcgcaggcgg cggcggtgag ccaggcgacc gatccgctga gcctgctgat tgaaaccgtg  600

acccaggcgc tgcaggcgct gaccattccg agctttattc cggaagattt tacctttctg  660

gatgcgattt ttgcgggcta tgcgaccgtg ggcgtgaccc aggatgtgga aagctttgtg  720

gcgggcacca ttggcgcgga aagcaacctg ggcctgctga acgtgggcga tgaaaacccg  780

gcggaagtga ccccgggcga ttttggcatt ggcgaactgg tgagcgcgac cagcccgggc  840

ggcggcgtga gcgcgagcgg cgcgggcggc gcggcgagcg tgggcaacac cgtgctggcg  900

agcgtgggcc gcgcgaacag cattggccag ctgagcgtgc cgccgagctg ggcggcgccg  960

agcacccgcc cggtgagcgc gctgagcccg gcgggcctga ccaccctgcc gggcaccgat  1020

gtggcggaac atggcatgcc gggcgtgccg ggcgtgccgg tggcggcggg ccgcgcgagc  1080

ggcgtgctgc cgcgctatgg cgtgcgcctg accgtgatgg cgcatccgcc ggcggcgggc  1140

gaatttatga cagagcagca gtggaatttc gcgggtatcg aggccgcggc aagcgcaatc  1200

cagggaaatg tcacgtccat tcattccctc cttgacgagg ggaagcagtc cctgaccaag  1260

ctcgcagcgg cctggggcgg tagcggttcg gaggcgtacc agggtgtcca gcaaaaatgg  1320

gacgccacgg ctaccgagct gaacaacgcg ctgcagaacc tggcgcggac gatcagcgaa  1380

gccggtcagg caatggcttc gaccgaaggc aacgtcactg ggatgttcgc agaattcatg  1440

attgcgacta cccgtgatcg tgagggcgcg accatgatca cgttccgtct gcgtctgccg  1500

tgtcgcacca ttttgcgcgt gttttcgcgt aacccgctgg tccgcggtac cgaccgtctg  1560

gaggccgttg tcatgctgct ggcggttacc gtgagcctgc tgacgatccc attcgcagcg  1620

gcagctggca cggccgtcca agacagccgt agccatgtgt atgctcacca ggctcaaacc  1680

cgtcacccgg ctactgccac tgttatcgat cacgaaggcg tgattgactc caataccacg  1740

gcaacctccg caccgcctcg caccaagatt acggttcctg cgcgttgggt ggtgaatggt  1800

attgaacgca gcggcgaagt taatgccaaa ccgggtacca aaagcggtga ccgtgtgggc  1860

atctgggtcg atagcgccgg tcagctggtc gacgagccgg caccgccagc gcgtgcgatc  1920

gccgatgcgg cgctggctgc cctgggtctg tggctgagcg tggcagcggt cgccggtgcg  1980

ttgctggcgc tgacgcgcgc aattctgatc cgcgttcgca atgcgagctg gcagcacgat  2040

attgatagcc tgttttgcac ccaacgtgag ctcatgacca cggcgcgtga tatcatgaat  2100

gcgggtgtca cctgtgttgg cgagcacgaa acgttgaccg cagcagcaca gtacatgcgc  2160

gaacatgata tcggcgcatt gccgatttgc ggcgacgatg atcgtctgca cggtatgctg  2220

accgaccgcg atatcgttat caagggtctg gccgcaggct tggacccgaa caccgcgacc  2280

gccggtgaac tggcacgtga cagcatctat tacgtcgacg cgaacgccag cattcaagag  2340

atgctgaacg tgatggaaga gcatcaggtg cgtcgtgtcc cggttatcag cgaacatcgt  2400

ctggttggta tcgttaccga agccgacatc gcacgtcacc tgccggagca cgcgattgtt  2460

cagttcgtga aagcgatttg cagcccgatg gcgttggcgt ctcgtcaaaa gggcgacaca  2520

aaatttattc taaatgcaaa gcttgcatgc aaaacggtga cgttgaccgt cgacggaacc  2580

gcgatgcggg tgaccacgat gaaatcgcgg gtgatcgaca tcgtcgaaga gaacgggttc  2640

tcagtcgacg accgcgacga cctgtatccc gcggccggcg tgcaggtcca tgacgccgac  2700

accatcgtgc tgcggcgtag ccgtccgctg cagatctcgc tggatggtca cgacgctaag  2760

caggtgtgga cgaccgcgtc gacggtggac gaggcgctgg cccaactcgc gatgaccgac  2820

acggcgccgg ccgcggcttc tcgcgccagc cgcgtcccgc tgtccgggat ggcgctaccg  2880

gtcgtcagcg ccaagacggt gcagctcaac gacggcgggt tggtgcgcac ggtgcacttg  2940

ccggccccca atgtcgcggg gctgctgagt gcggccggcg tgccgctgtt gcaaagcgac  3000

cacgtggtgc ccgccgcgac ggccccgatc gtcgaaggca tgcagatcca ggtgacccgc  3060

aatcggatca agaaggtcac cgagcggctg ccgctgccgc cgaacgcgcg tcgtgtcgag  3120

gacccggaga tgaacatgag ccgggaggtc gtcgaagacc cgggggttcc ggggacccag  3180

gatgtgacgt tcgcggtagc tgaggtcaac ggcgtcgaga ccggccgttt gcccgtcgcc  3240

aacgtcgtgg tgaccccggc ccacgaagcc gtggtgcggg tgggcaccaa gcccggtacc  3300

gaggtgcccc cggtgatcga cggaagcatc tgggacgcga tcgccggctg tgaggccggt  3360

ggcaactggg cgatcaacac cggcaacggg tattacggtg gtgtgcagtt tgaccagggc  3420

acctgggagg ccaacggcgg gctgcggtat gcaccccgcg ctgacctcgc cacccgcgaa  3480

gagcagatcg ccgttgccga ggtgacccga ctgcgtcaag gttggggcgc ctggccggta  3540

tgtgctgcac gagcgggtgc gcgctga                                      3567


<210> 74
<211> 1091
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: PPE51-Rv1733c-Rv2626c-RpfB 

<400> 74
Met Asp Phe Ala Leu Leu Pro Pro Glu Val Asn Ser Ala Arg Met Tyr 
1               5                   10                  15

Thr Gly Pro Gly Ala Gly Ser Leu Leu Ala Ala Ala Gly Gly Trp Asp 
            20                  25                  30

Ser Leu Ala Ala Glu Leu Ala Thr Thr Ala Glu Ala Tyr Gly Ser Val 
        35                  40                  45

Leu Ser Gly Leu Ala Ala Leu His Trp Arg Gly Pro Ala Ala Glu Ser 
    50                  55                  60

Met Ala Val Thr Ala Ala Pro Tyr Ile Gly Trp Leu Tyr Thr Thr Ala 
65                  70                  75                  80

Glu Lys Thr Gln Gln Thr Ala Ile Gln Ala Arg Ala Ala Ala Leu Ala 
                85                  90                  95

Phe Glu Gln Ala Tyr Ala Met Thr Leu Pro Pro Pro Val Val Ala Ala 
            100                 105                 110

Asn Arg Ile Gln Leu Leu Ala Leu Ile Ala Thr Asn Phe Phe Gly Gln 
        115                 120                 125

Asn Thr Ala Ala Ile Ala Ala Thr Glu Ala Gln Tyr Ala Glu Met Trp 
    130                 135                 140

Ala Gln Asp Ala Ala Ala Met Tyr Gly Tyr Ala Thr Ala Ser Ala Ala 
145                 150                 155                 160

Ala Ala Leu Leu Thr Pro Phe Ser Pro Pro Arg Gln Thr Thr Asn Pro 
                165                 170                 175

Ala Gly Leu Thr Ala Gln Ala Ala Ala Val Ser Gln Ala Thr Asp Pro 
            180                 185                 190

Leu Ser Leu Leu Ile Glu Thr Val Thr Gln Ala Leu Gln Ala Leu Thr 
        195                 200                 205

Ile Pro Ser Phe Ile Pro Glu Asp Phe Thr Phe Leu Asp Ala Ile Phe 
    210                 215                 220

Ala Gly Tyr Ala Thr Val Gly Val Thr Gln Asp Val Glu Ser Phe Val 
225                 230                 235                 240

Ala Gly Thr Ile Gly Ala Glu Ser Asn Leu Gly Leu Leu Asn Val Gly 
                245                 250                 255

Asp Glu Asn Pro Ala Glu Val Thr Pro Gly Asp Phe Gly Ile Gly Glu 
            260                 265                 270

Leu Val Ser Ala Thr Ser Pro Gly Gly Gly Val Ser Ala Ser Gly Ala 
        275                 280                 285

Gly Gly Ala Ala Ser Val Gly Asn Thr Val Leu Ala Ser Val Gly Arg
    290                 295                 300

Ala Asn Ser Ile Gly Gln Leu Ser Val Pro Pro Ser Trp Ala Ala Pro 
305                 310                 315                 320

Ser Thr Arg Pro Val Ser Ala Leu Ser Pro Ala Gly Leu Thr Thr Leu 
                325                 330                 335

Pro Gly Thr Asp Val Ala Glu His Gly Met Pro Gly Val Pro Gly Val 
            340                 345                 350

Pro Val Ala Ala Gly Arg Ala Ser Gly Val Leu Pro Arg Tyr Gly Val 
        355                 360                 365

Arg Leu Thr Val Met Ala His Pro Pro Ala Ala Gly Glu Phe Met Ile 
    370                 375                 380

Ala Thr Thr Arg Asp Arg Glu Gly Ala Thr Met Ile Thr Phe Arg Leu 
385                 390                 395                 400

Arg Leu Pro Cys Arg Thr Ile Leu Arg Val Phe Ser Arg Asn Pro Leu 
                405                 410                 415

Val Arg Gly Thr Asp Arg Leu Glu Ala Val Val Met Leu Leu Ala Val 
            420                 425                 430

Thr Val Ser Leu Leu Thr Ile Pro Phe Ala Ala Ala Ala Gly Thr Ala 
        435                 440                 445

Val Gln Asp Ser Arg Ser His Val Tyr Ala His Gln Ala Gln Thr Arg 
    450                 455                 460

His Pro Ala Thr Ala Thr Val Ile Asp His Glu Gly Val Ile Asp Ser 
465                 470                 475                 480

Asn Thr Thr Ala Thr Ser Ala Pro Pro Arg Thr Lys Ile Thr Val Pro 
                485                 490                 495

Ala Arg Trp Val Val Asn Gly Ile Glu Arg Ser Gly Glu Val Asn Ala 
            500                 505                 510

Lys Pro Gly Thr Lys Ser Gly Asp Arg Val Gly Ile Trp Val Asp Ser 
        515                 520                 525

Ala Gly Gln Leu Val Asp Glu Pro Ala Pro Pro Ala Arg Ala Ile Ala 
    530                 535                 540

Asp Ala Ala Leu Ala Ala Leu Gly Leu Trp Leu Ser Val Ala Ala Val 
545                 550                 555                 560

Ala Gly Ala Leu Leu Ala Leu Thr Arg Ala Ile Leu Ile Arg Val Arg 
                565                 570                 575

Asn Ala Ser Trp Gln His Asp Ile Asp Ser Leu Phe Cys Thr Gln Arg 
            580                 585                 590

Glu Leu Met Thr Thr Ala Arg Asp Ile Met Asn Ala Gly Val Thr Cys 
        595                 600                 605

Val Gly Glu His Glu Thr Leu Thr Ala Ala Ala Gln Tyr Met Arg Glu 
    610                 615                 620

His Asp Ile Gly Ala Leu Pro Ile Cys Gly Asp Asp Asp Arg Leu His 
625                 630                 635                 640

Gly Met Leu Thr Asp Arg Asp Ile Val Ile Lys Gly Leu Ala Ala Gly 
                645                 650                 655

Leu Asp Pro Asn Thr Ala Thr Ala Gly Glu Leu Ala Arg Asp Ser Ile 
            660                 665                 670

Tyr Tyr Val Asp Ala Asn Ala Ser Ile Gln Glu Met Leu Asn Val Met 
        675                 680                 685

Glu Glu His Gln Val Arg Arg Val Pro Val Ile Ser Glu His Arg Leu 
    690                 695                 700

Val Gly Ile Val Thr Glu Ala Asp Ile Ala Arg His Leu Pro Glu His 
705                 710                 715                 720

Ala Ile Val Gln Phe Val Lys Ala Ile Cys Ser Pro Met Ala Leu Ala 
                725                 730                 735

Ser Arg Gln Lys Gly Asp Thr Lys Phe Ile Leu Asn Ala Lys Leu Ala 
            740                 745                 750

Cys Lys Thr Val Thr Leu Thr Val Asp Gly Thr Ala Met Arg Val Thr 
        755                 760                 765

Thr Met Lys Ser Arg Val Ile Asp Ile Val Glu Glu Asn Gly Phe Ser 
    770                 775                 780

Val Asp Asp Arg Asp Asp Leu Tyr Pro Ala Ala Gly Val Gln Val His 
785                 790                 795                 800

Asp Ala Asp Thr Ile Val Leu Arg Arg Ser Arg Pro Leu Gln Ile Ser 
                805                 810                 815

Leu Asp Gly His Asp Ala Lys Gln Val Trp Thr Thr Ala Ser Thr Val 
            820                 825                 830

Asp Glu Ala Leu Ala Gln Leu Ala Met Thr Asp Thr Ala Pro Ala Ala 
        835                 840                 845

Ala Ser Arg Ala Ser Arg Val Pro Leu Ser Gly Met Ala Leu Pro Val 
    850                 855                 860

Val Ser Ala Lys Thr Val Gln Leu Asn Asp Gly Gly Leu Val Arg Thr 
865                 870                 875                 880

Val His Leu Pro Ala Pro Asn Val Ala Gly Leu Leu Ser Ala Ala Gly 
                885                 890                 895

Val Pro Leu Leu Gln Ser Asp His Val Val Pro Ala Ala Thr Ala Pro 
            900                 905                 910

Ile Val Glu Gly Met Gln Ile Gln Val Thr Arg Asn Arg Ile Lys Lys 
        915                 920                 925

Val Thr Glu Arg Leu Pro Leu Pro Pro Asn Ala Arg Arg Val Glu Asp 
    930                 935                 940

Pro Glu Met Asn Met Ser Arg Glu Val Val Glu Asp Pro Gly Val Pro 
945                 950                 955                 960

Gly Thr Gln Asp Val Thr Phe Ala Val Ala Glu Val Asn Gly Val Glu 
                965                 970                 975

Thr Gly Arg Leu Pro Val Ala Asn Val Val Val Thr Pro Ala His Glu 
            980                 985                 990

Ala Val Val Arg Val Gly Thr Lys Pro Gly Thr Glu Val Pro Pro Val 
        995                 1000                1005

Ile Asp Gly Ser Ile Trp Asp Ala Ile Ala Gly Cys Glu Ala Gly Gly 
    1010                1015                1020

Asn Trp Ala Ile Asn Thr Gly Asn Gly Tyr Tyr Gly Gly Val Gln Phe 
1025                1030                1035                1040

Asp Gln Gly Thr Trp Glu Ala Asn Gly Gly Leu Arg Tyr Ala Pro Arg 
                1045                1050                1055

Ala Asp Leu Ala Thr Arg Glu Glu Gln Ile Ala Val Ala Glu Val Thr 
            1060                1065                1070

Arg Leu Arg Gln Gly Trp Gly Ala Trp Pro Val Cys Ala Ala Arg Ala 
        1075                1080                1085

Gly Ala Arg
    1090


<210> 75
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 85B For NdeI primer

<400> 75
atagatcata tgtttagccg tcctggcctg c     31


<210> 76
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 85B Rev EcoRI nostop primer

<400> 76
ttaagagaat tcgcccgcac ccagagagga t     31


<210> 77
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT-6 For BamHI primer

<400> 77
aacgttggat ccatgacaga gcagcagtgg aa     32


<210> 78
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT-6 Rev EcoRI ns primer

<400> 78
atactagaat tctgcgaaca tcccagtgac gt     32


<210> 79
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 1733 For EcoRI primer

<400> 79
aacttagaat tcatgattgc gactacccgt gat     33


<210> 80
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 1733 In1 Rev Xma primer

<400> 80
gatatacccg ggggcctcca gacggtcggt     30


<210> 81
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 1733 Out For Xma primer

<400> 81
aacgaacccg gggtccaaga cagccgtagc c     31

<210> 82
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 1733 Out Rev Xba primer

<400> 82
taagtatcta gaatcggcga tcgcacgcgc t     31


<210> 83
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 1733 In2 For Xba primer

<400> 83
atagaatcta gacgcgcaat tctgatccgc gt     32


<210> 84
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 1733 Rev ns SacI primer

<400> 84
agataagagc tcacgttggg tgcaaaacag gc     32


<210> 85
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 2626 For SacI primer 

<400> 85
atagaagagc tcatgaccac ggcgcgtgat a     31


<210> 86
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: 2626 Rev HindIII ns primer

<400> 86
taaagaaagc tttgcattta gaataaattt tgtgtc     36


<210> 87
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfD For HindIII primer

<400> 87
taactaaagc ttttgctggg cctgagcacc     30


<210> 88
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: RpfD Rev XhoI stop primer

<400> 88
atctaactcga gctagtcatc gcggctaccg ct     32


<210> 89
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT6 For NdeI primer

<400> 89
taagatcatat gacagagcag cagtggaatt tc     32


<210> 90
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: ESAT-6 Rev EcoRI ns primer

<400> 90
atactagaat tctgcgaaca tcccagtgac gt     32


<210> 91
<211> 1263
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Ag85B-ESAT6 fusion protein with
      Ag85B signal sequence

<400> 91
atgacagacg tgagccgaaa gattcgagct tggggacgcc gattgatgat cggcacggca  60

gcggctgtag tccttccggg cctggtgggg cttgccggcg gagcggcaac cgcgggcgcg  120

ttctcccggc cggggctgcc ggtcgagtac ctgcaggtgc cgtcgccgtc gatgggccgc  180

gacatcaagg ttcagttcca gagcggtggg aacaactcac ctgcggttta tctgctcgac  240

ggcctgcgcg cccaagacga ctacaacggc tgggatatca acaccccggc gttcgagtgg  300

tactaccagt cgggactgtc gatagtcatg ccggtcggcg ggcagtccag cttctacagc  360

gactggtaca gcccggcctg cggtaaggct ggctgccaga cttacaagtg ggaaaccttc  420

ctgaccagcg agctgccgca atggttgtcc gccaacaggg ccgtgaagcc caccggcagc  480

gctgcaatcg gcttgtcgat ggccggctcg tcggcaatga tcttggccgc ctaccacccc  540

cagcagttca tctacgccgg ctcgctgtcg gccctgctgg acccctctca ggggatgggg  600

cctagcctga tcggcctcgc gatgggtgac gccggcggtt acaaggccgc agacatgtgg  660

ggtccctcga gtgacccggc atgggagcgc aacgacccta cgcagcagat ccccaagctg  720

gtcgcaaaca acacccggct atgggtttat tgcgggaacg gcaccccgaa cgagttgggc  780

ggtgccaaca tacccgccga gttcttggag aacttcgttc gtagcagcaa cctgaagttc  840

caggatgcgt acaacgccgc gggcgggcac aacgccgtgt tcaacttccc gcccaacggc  900

acgcacagct gggagtactg gggcgctcag ctcaacgcca tgaagggtga cctgcagagt  960

tcgttaggcg ccggcatgac agagcagcag tggaatttcg cgggtatcga ggccgcggca  1020

agcgcaatcc agggaaatgt cacgtccatt cattccctcc ttgacgaggg gaagcagtcc  1080

ctgaccaagc tcgcagcggc ctggggcggt agcggttcgg aggcgtacca gggtgtccag  1140

caaaaatggg acgccacggc taccgagctg aacaacgcgc tgcagaacct ggcgcggacg  1200

atcagcgaag ccggtcaggc aatggcttcg accgaaggca acgtcactgg gatgttcgca  1260

tga                                                                1263


<210> 92
<211> 420
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Ag85B-ESAT6 (Ag85B signal 
      sequence)

<400> 92
Met Thr Asp Val Ser Arg Lys Ile Arg Ala Trp Gly Arg Arg Leu Met 
1               5                   10                  15

Ile Gly Thr Ala Ala Ala Val Val Leu Pro Gly Leu Val Gly Leu Ala 
            20                  25                  30

Gly Gly Ala Ala Thr Ala Gly Ala Phe Ser Arg Pro Gly Leu Pro Val 
        35                  40                  45

Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp Ile Lys Val 
    50                  55                  60

Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr Leu Leu Asp 
65                  70                  75                  80

Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile Asn Thr Pro 
                85                  90                  95

Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val Met Pro Val 
            100                 105                 110

Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro Ala Cys Gly 
        115                 120                 125

Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu Thr Ser Glu 
    130                 135                 140

Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro Thr Gly Ser 
145                 150                 155                 160

Ala Ala Ile Gly Leu Ser Met Ala Gly Ser Ser Ala Met Ile Leu Ala 
                165                 170                 175

Ala Tyr His Pro Gln Gln Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu 
            180                 185                 190

Leu Asp Pro Ser Gln Gly Met Gly Pro Ser Leu Ile Gly Leu Ala Met 
        195                 200                 205

Gly Asp Ala Gly Gly Tyr Lys Ala Ala Asp Met Trp Gly Pro Ser Ser 
    210                 215                 220

Asp Pro Ala Trp Glu Arg Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu 
225                 230                 235                 240

Val Ala Asn Asn Thr Arg Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro 
                245                 250                 255

Asn Glu Leu Gly Gly Ala Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe 
            260                 265                 270

Val Arg Ser Ser Asn Leu Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly 
        275                 280                 285

Gly His Asn Ala Val Phe Asn Phe Pro Pro Asn Gly Thr His Ser Trp 
    290                 295                 300

Glu Tyr Trp Gly Ala Gln Leu Asn Ala Met Lys Gly Asp Leu Gln Ser 
305                 310                 315                 320

Ser Leu Gly Ala Gly Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile 
                325                 330                 335

Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser 
            340                 345                 350

Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp 
        355                 360                 365

Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp 
    370                 375                 380

Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr 
385                 390                 395                 400

Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr 
                405                 410                 415

Gly Met Phe Ala 
            420

<210> 93
<211> 1272
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Ag85B-ESAT6 fusion protein with
      19 kDa lipoprotein signal sequence

<400> 93
atgaagcgtg gactgacggt cgcggtagcc ggagccgcca ttctggtcgc aggtctttcc  60

ggatgttcaa gcaacaagtc gactacagga agcggtgaga ccacgaccgc ggcaggtacc  120

acggcaagcc ccggccggcc ggggctgccg gtcgagtacc tgcaggtgcc gtcgccgtcg  180

atgggccgcg acatcaaggt tcagttccag agcggtggga acaactcacc tgcggtttat  240

ctgctcgacg gcctgcgcgc ccaagacgac tacaacggct gggatatcaa caccccggcg  300

ttcgagtggt actaccagtc gggactgtcg atagtcatgc cggtcggcgg gcagtccagc  360

ttctacagcg actggtacag cccggcctgc ggtaaggctg gctgccagac ttacaagtgg  420

gaaaccttcc tgaccagcga gctgccgcaa tggttgtccg ccaacagggc cgtgaagccc  480

accggcagcg ctgcaatcgg cttgtcgatg gccggctcgt cggcaatgat cttggccgcc  540

taccaccccc agcagttcat ctacgccggc tcgctgtcgg ccctgctgga cccctctcag  600

gggatggggc ctagcctgat cggcctcgcg atgggtgacg ccggcggtta caaggccgca  660

gacatgtggg gtccctcgag tgacccggca tgggagcgca acgaccctac gcagcagatc  720

cccaagctgg tcgcaaacaa cacccggcta tgggtttatt gcgggaacgg caccccgaac  780

gagttgggcg gtgccaacat acccgccgag ttcttggaga acttcgttcg tagcagcaac  840

ctgaagttcc aggatgcgta caacgccgcg ggcgggcaca acgccgtgtt caacttcccg  900

cccaacggca cgcacagctg ggagtactgg ggcgctcagc tcaacgccat gaagggtgac  960

ctgcagagtt cgttaggcgc cggcatgaca gagcagcagt ggaatttcgc gggtatcgag  1020

gccgcggcaa gcgcaatcca gggaaatgtc acgtccattc attccctcct tgacgagggg  1080

aagcagtccc tgaccaagct cgcagcggcc tggggcggta gcggttcgga ggcgtaccag  1140

ggtgtccagc aaaaatggga cgccacggct accgagctga acaacgcgct gcagaacctg  1200

gcgcggacga tcagcgaagc cggtcaggca atggcttcga ccgaaggcaa cgtcactggg  1260

atgttcgcat ga                                                      1272


<210> 94
<211> 423
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Ag85B-ESAT6 (19 kDa lipoprotein 
      signal sequence)

<400> 94
Met Lys Arg Gly Leu Thr Val Ala Val Ala Gly Ala Ala Ile Leu Val 
1               5                   10                  15

Ala Gly Leu Ser Gly Cys Ser Ser Asn Lys Ser Thr Thr Gly Ser Gly 
            20                  25                  30

Glu Thr Thr Thr Ala Ala Gly Thr Thr Ala Ser Pro Gly Arg Pro Gly 
        35                  40                  45

Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp 
    50                  55                  60

Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr 
65                  70                  75                  80

Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile 
                85                  90                  95

Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val 
            100                 105                 110

Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro 
        115                 120                 125

Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu 
    130                 135                 140

Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro 
145                 150                 155                 160

Thr Gly Ser Ala Ala Ile Gly Leu Ser Met Ala Gly Ser Ser Ala Met 
                165                 170                 175

Ile Leu Ala Ala Tyr His Pro Gln Gln Phe Ile Tyr Ala Gly Ser Leu 
            180                 185                 190

Ser Ala Leu Leu Asp Pro Ser Gln Gly Met Gly Pro Ser Leu Ile Gly 
        195                 200                 205

Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys Ala Ala Asp Met Trp Gly 
    210                 215                 220

Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn Asp Pro Thr Gln Gln Ile 
225                 230                 235                 240

Pro Lys Leu Val Ala Asn Asn Thr Arg Leu Trp Val Tyr Cys Gly Asn 
                245                 250                 255

Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn Ile Pro Ala Glu Phe Leu 
            260                 265                 270

Glu Asn Phe Val Arg Ser Ser Asn Leu Lys Phe Gln Asp Ala Tyr Asn 
        275                 280                 285

Ala Ala Gly Gly His Asn Ala Val Phe Asn Phe Pro Pro Asn Gly Thr 
    290                 295                 300

His Ser Trp Glu Tyr Trp Gly Ala Gln Leu Asn Ala Met Lys Gly Asp 
305                 310                 315                 320

Leu Gln Ser Ser Leu Gly Ala Gly Met Thr Glu Gln Gln Trp Asn Phe 
                325                 330                 335

Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser 
            340                 345                 350

Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala 
        355                 360                 365

Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln 
    370                 375                 380

Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu 
385                 390                 395                 400

Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly 
                405                 410                 415

Asn Val Thr Gly Met Phe Ala 
            420

<210> 95
<211> 1020
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv2626c-RpfD fusion protein
      with Ag85B signal sequence

<400> 95
atgacagacg tgagccgaaa gattcgagct tggggacgcc gattgatgat cggcacggca  60

gcggctgtag tccttccggg cctggtgggg cttgccggcg gagcggcaac cgcgggcgcg  120

ttctccatga ccaccgcacg cgacatcatg aacgcaggtg tgacctgtgt tggcgaacac  180

gagacgctaa ccgctgccgc tcaatacatg cgtgagcacg acatcggcgc gttgccgatc  240

tgcggggacg acgaccggct gcacggcatg ctcaccgacc gcgacattgt gatcaaaggc  300

ctggctgcgg gcctagaccc gaataccgcc acggctggcg agttggcccg ggacagcatc  360

tactacgtcg atgcgaacgc aagcatccag gagatgctca acgtcatgga agaacatcag  420

gtccgccgtg ttccggtcat ctcagagcac cgcttggtcg gaatcgtcac cgaagccgac  480

atcgcccgac acctgcccga gcacgccatt gtgcagttcg tcaaggcaat ctgctcgccc  540

atggccctcg ccagcatgac accgggtttg cttactactg cgggtgctgg ccgaccacgt  600

gacaggtgcg ccaggatcgt atgcacggtg ttcatcgaaa ccgccgttgt cgcgaccatg  660

tttgtcgcgt tgttgggtct gtccaccatc agctcgaaag ccgacgacat cgattgggac  720

gccatcgcgc aatgcgaatc cggcggcaat tgggcggcca acaccggtaa cgggttatac  780

ggtggtctgc agatcagcca ggcgacgtgg gattccaacg gtggtgtcgg gtcgccggcg  840

gccgcgagtc cccagcaaca gatcgaggtc gcagacaaca ttatgaaaac ccaaggcccg  900

ggtgcgtggc cgaaatgtag ttcttgtagt cagggagacg caccgctggg ctcgctcacc  960

cacatcctga cgttcctcgc ggccgagact ggaggttgtt cggggagcag ggacgattag  1020


<210> 96
<211> 339
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv2626c-RpfD (Ag85B signal 
      sequence)

<400> 96
Met Thr Asp Val Ser Arg Lys Ile Arg Ala Trp Gly Arg Arg Leu Met 
1               5                   10                  15

Ile Gly Thr Ala Ala Ala Val Val Leu Pro Gly Leu Val Gly Leu Ala 
            20                  25                  30

Gly Gly Ala Ala Thr Ala Gly Ala Phe Ser Met Thr Thr Ala Arg Asp 
        35                  40                  45

Ile Met Asn Ala Gly Val Thr Cys Val Gly Glu His Glu Thr Leu Thr 
    50                  55                  60

Ala Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly Ala Leu Pro Ile 
65                  70                  75                  80

Cys Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr Asp Arg Asp Ile 
                85                  90                  95

Val Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn Thr Ala Thr Ala 
            100                 105                 110

Gly Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp Ala Asn Ala Ser 
        115                 120                 125

Ile Gln Glu Met Leu Asn Val Met Glu Glu His Gln Val Arg Arg Val 
    130                 135                 140

Pro Val Ile Ser Glu His Arg Leu Val Gly Ile Val Thr Glu Ala Asp 
145                 150                 155                 160

Ile Ala Arg His Leu Pro Glu His Ala Ile Val Gln Phe Val Lys Ala 
                165                 170                 175

Ile Cys Ser Pro Met Ala Leu Ala Ser Met Thr Pro Gly Leu Leu Thr 
            180                 185                 190

Thr Ala Gly Ala Gly Arg Pro Arg Asp Arg Cys Ala Arg Ile Val Cys 
        195                 200                 205

Thr Val Phe Ile Glu Thr Ala Val Val Ala Thr Met Phe Val Ala Leu 
    210                 215                 220

Leu Gly Leu Ser Thr Ile Ser Ser Lys Ala Asp Asp Ile Asp Trp Asp 
225                 230                 235                 240

Ala Ile Ala Gln Cys Glu Ser Gly Gly Asn Trp Ala Ala Asn Thr Gly 
                245                 250                 255

Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Thr Trp Asp Ser 
            260                 265                 270

Asn Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln Gln Gln Ile 
        275                 280                 285

Glu Val Ala Asp Asn Ile Met Lys Thr Gln Gly Pro Gly Ala Trp Pro 
    290                 295                 300

Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly Ser Leu Thr 
305                 310                 315                 320

His Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly Cys Ser Gly Ser 
                325                 330                 335

Arg Asp Asp 


<210> 97
<211> 1029
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv2626c-RpfD fusion protein
      with 19 kDa lipoprotein signal sequence

<400> 97
atgaagcgtg gactgacggt cgcggtagcc ggagccgcca ttctggtcgc aggtctttcc  60

ggatgttcaa gcaacaagtc gactacagga agcggtgaga ccacgaccgc ggcaggtacc  120

acggcaagcc ccggcatgac caccgcacgc gacatcatga acgcaggtgt gacctgtgtt  180

ggcgaacacg agacgctaac cgctgccgct caatacatgc gtgagcacga catcggcgcg  240

ttgccgatct gcggggacga cgaccggctg cacggcatgc tcaccgaccg cgacattgtg  300

atcaaaggcc tggctgcggg cctagacccg aataccgcca cggctggcga gttggcccgg  360

gacagcatct actacgtcga tgcgaacgca agcatccagg agatgctcaa cgtcatggaa  420

gaacatcagg tccgccgtgt tccggtcatc tcagagcacc gcttggtcgg aatcgtcacc  480

gaagccgaca tcgcccgaca cctgcccgag cacgccattg tgcagttcgt caaggcaatc  540

tgctcgccca tggccctcgc cagcatgaca ccgggtttgc ttactactgc gggtgctggc  600

cgaccacgtg acaggtgcgc caggatcgta tgcacggtgt tcatcgaaac cgccgttgtc  660

gcgaccatgt ttgtcgcgtt gttgggtctg tccaccatca gctcgaaagc cgacgacatc  720

gattgggacg ccatcgcgca atgcgaatcc ggcggcaatt gggcggccaa caccggtaac  780

gggttatacg gtggtctgca gatcagccag gcgacgtggg attccaacgg tggtgtcggg  840

tcgccggcgg ccgcgagtcc ccagcaacag atcgaggtcg cagacaacat tatgaaaacc  900

caaggcccgg gtgcgtggcc gaaatgtagt tcttgtagtc agggagacgc accgctgggc  960

tcgctcaccc acatcctgac gttcctcgcg gccgagactg gaggttgttc ggggagcagg  1020

gacgattag                                                          1029


<210> 98
<211> 342
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Rv2626c-RpfD (19 kDa 
      lipoprotein signal sequence)

<400> 98
Met Lys Arg Gly Leu Thr Val Ala Val Ala Gly Ala Ala Ile Leu Val 
1               5                   10                  15

Ala Gly Leu Ser Gly Cys Ser Ser Asn Lys Ser Thr Thr Gly Ser Gly 
            20                  25                  30

Glu Thr Thr Thr Ala Ala Gly Thr Thr Ala Ser Pro Gly Met Thr Thr 
        35                  40                  45

Ala Arg Asp Ile Met Asn Ala Gly Val Thr Cys Val Gly Glu His Glu 
    50                  55                  60

Thr Leu Thr Ala Ala Ala Gln Tyr Met Arg Glu His Asp Ile Gly Ala 
65                  70                  75                  80

Leu Pro Ile Cys Gly Asp Asp Asp Arg Leu His Gly Met Leu Thr Asp 
                85                  90                  95

Arg Asp Ile Val Ile Lys Gly Leu Ala Ala Gly Leu Asp Pro Asn Thr 
            100                 105                 110

Ala Thr Ala Gly Glu Leu Ala Arg Asp Ser Ile Tyr Tyr Val Asp Ala 
        115                 120                 125

Asn Ala Ser Ile Gln Glu Met Leu Asn Val Met Glu Glu His Gln Val 
    130                 135                 140

Arg Arg Val Pro Val Ile Ser Glu His Arg Leu Val Gly Ile Val Thr 
145                 150                 155                 160

Glu Ala Asp Ile Ala Arg His Leu Pro Glu His Ala Ile Val Gln Phe 
                165                 170                 175

Val Lys Ala Ile Cys Ser Pro Met Ala Leu Ala Ser Met Thr Pro Gly 
            180                 185                 190

Leu Leu Thr Thr Ala Gly Ala Gly Arg Pro Arg Asp Arg Cys Ala Arg 
        195                 200                 205

Ile Val Cys Thr Val Phe Ile Glu Thr Ala Val Val Ala Thr Met Phe 
    210                 215                 220

Val Ala Leu Leu Gly Leu Ser Thr Ile Ser Ser Lys Ala Asp Asp Ile 
225                 230                 235                 240

Asp Trp Asp Ala Ile Ala Gln Cys Glu Ser Gly Gly Asn Trp Ala Ala 
                245                 250                 255

Asn Thr Gly Asn Gly Leu Tyr Gly Gly Leu Gln Ile Ser Gln Ala Thr 
            260                 265                 270

Trp Asp Ser Asn Gly Gly Val Gly Ser Pro Ala Ala Ala Ser Pro Gln 
        275                 280                 285

Gln Gln Ile Glu Val Ala Asp Asn Ile Met Lys Thr Gln Gly Pro Gly 
    290                 295                 300

Ala Trp Pro Lys Cys Ser Ser Cys Ser Gln Gly Asp Ala Pro Leu Gly 
305                 310                 315                 320

Ser Leu Thr His Ile Leu Thr Phe Leu Ala Ala Glu Thr Gly Gly Cys 
                325                 330                 335

Ser Gly Ser Arg Asp Asp 
            340


