                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  SUBTILASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

<130>  12720-WO-PCT

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1149
<212>  DNA
<213>  Bacillus amyloliquefaciens


<220>
<221>  CDS
<222>  (1)..(1146)

<220>
<221>  sig_peptide
<222>  (1)..(90)

<220>
<221>  mat_peptide
<222>  (322)..(1146)

<400>  1
atg aga ggc  aaa aaa gta tgg atc  agt ttg ctg ttt gct tta gcg tta       48
Met Arg Gly  Lys Lys Val Trp Ile  Ser Leu Leu Phe Ala Leu Ala Leu         
        -105                 -100                 -95                     

atc ttt acg atg gcg ttc ggc agc aca tcc tct gcc cag gcg gca ggg         96
Ile Phe Thr Met Ala Phe Gly Ser Thr Ser Ser Ala Gln Ala Ala Gly           
    -90                 -85                 -80                           

aaa tca aac ggg gaa aag aaa tat att gtc ggg ttt aaa cag aca atg        144
Lys Ser Asn Gly Glu Lys Lys Tyr Ile Val Gly Phe Lys Gln Thr Met           
-75                 -70                 -65                 -60           

agc acg atg agc gcc gct aag aag aaa gat gtc att tct gaa aaa ggc        192
Ser Thr Met Ser Ala Ala Lys Lys Lys Asp Val Ile Ser Glu Lys Gly           
                -55                 -50                 -45               

ggg aaa gtg caa aag caa ttc aaa tat gta gac gca gct tca gct aca        240
Gly Lys Val Gln Lys Gln Phe Lys Tyr Val Asp Ala Ala Ser Ala Thr           
            -40                 -35                 -30                   

tta aac gaa aaa gct gta aaa gaa ttg aaa aaa gac ccg agc gtc gct        288
Leu Asn Glu Lys Ala Val Lys Glu Leu Lys Lys Asp Pro Ser Val Ala           
        -25                 -20                 -15                       

tac gtt gaa gaa gat cac gta gca cat gcg tac gcg cag tcc gtg cct        336
Tyr Val Glu Glu Asp His Val Ala His Ala Tyr Ala Gln Ser Val Pro           
    -10                 -5              -1  1               5             

tac ggc gta tca caa att aaa gcc cct gct ctg cac tct caa ggc tac        384
Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu His Ser Gln Gly Tyr           
                10                  15                  20                

act gga tca aat gtt aaa gta gcg gtt atc gac agc ggt atc gat tct        432
Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp Ser Gly Ile Asp Ser           
            25                  30                  35                    

tct cat cct gat tta aag gta gca ggc gga gcc agc atg gtt cct tct        480
Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala Ser Met Val Pro Ser           
        40                  45                  50                        

gaa aca aat cct ttc caa gac aac aac tct cac gga act cac gtt gcc        528
Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His Gly Thr His Val Ala           
    55                  60                  65                            

ggc aca gtt gcg gct ctt aat aac tca atc ggt gta tta ggc gtt gcg        576
Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Leu Gly Val Ala           
70                  75                  80                  85            

cca agc gca tca ctt tac gct gta aaa gtt ctc ggt gct gac ggt tcc        624
Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu Gly Ala Asp Gly Ser           
                90                  95                  100               

ggc caa tac agc tgg atc att aac gga atc gag tgg gcg atc gca aac        672
Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu Trp Ala Ile Ala Asn           
            105                 110                 115                   

aat atg gac gtt att aac atg agc ctc ggc gga cct tct ggt tct gct        720
Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro Ser Gly Ser Ala           
        120                 125                 130                       

gct tta aaa gcg gca gtt gat aaa gcc gtt gca tcc ggc gtc gta gtc        768
Ala Leu Lys Ala Ala Val Asp Lys Ala Val Ala Ser Gly Val Val Val           
    135                 140                 145                           

gtt gcg gca gcc ggt aac gaa ggc act tcc ggc agc tca agc aca gtg        816
Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly Ser Ser Ser Thr Val           
150                 155                 160                 165           

ggc tac cct ggt aaa tac cct tct gtc att gca gta ggc gct gtt gac        864
Gly Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala Val Gly Ala Val Asp           
                170                 175                 180               

agc agc aac caa aga gca tct ttc tca agc gta gga cct gag ctt gat        912
Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Val Gly Pro Glu Leu Asp           
            185                 190                 195                   

gtc atg gca cct ggc gta tct atc caa agc acg ctt cct gga aac aaa        960
Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr Leu Pro Gly Asn Lys           
        200                 205                 210                       

tac ggg gcg tac aac ggt acg tca atg gca tct ccg cac gtt gcc gga       1008
Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Ser Pro His Val Ala Gly           
    215                 220                 225                           

gcg gct gct ttg att ctt tct aag cac ccg aac tgg aca aac act caa       1056
Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Trp Thr Asn Thr Gln           
230                 235                 240                 245           

gtc cgc agc agt tta gaa aac acc act aca aaa ctt ggt gat tct ttc       1104
Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys Leu Gly Asp Ser Phe           
                250                 255                 260               

tac tat gga aaa ggg ctg atc aac gta cag gcg gca gct cag taa           1149
Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala Ala Ala Gln                   
            265                 270                 275                   


<210>  2
<211>  382
<212>  PRT
<213>  Bacillus amyloliquefaciens

<400>  2

Met Arg Gly  Lys Lys Val Trp Ile  Ser Leu Leu Phe Ala Leu Ala Leu 
        -105                 -100                 -95             


Ile Phe Thr Met Ala Phe Gly Ser Thr Ser Ser Ala Gln Ala Ala Gly 
    -90                 -85                 -80                 


Lys Ser Asn Gly Glu Lys Lys Tyr Ile Val Gly Phe Lys Gln Thr Met 
-75                 -70                 -65                 -60 


Ser Thr Met Ser Ala Ala Lys Lys Lys Asp Val Ile Ser Glu Lys Gly 
                -55                 -50                 -45     


Gly Lys Val Gln Lys Gln Phe Lys Tyr Val Asp Ala Ala Ser Ala Thr 
            -40                 -35                 -30         


Leu Asn Glu Lys Ala Val Lys Glu Leu Lys Lys Asp Pro Ser Val Ala 
        -25                 -20                 -15             


Tyr Val Glu Glu Asp His Val Ala His Ala Tyr Ala Gln Ser Val Pro 
    -10                 -5              -1  1               5   


Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu His Ser Gln Gly Tyr 
                10                  15                  20      


Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp Ser Gly Ile Asp Ser 
            25                  30                  35          


Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala Ser Met Val Pro Ser 
        40                  45                  50              


Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His Gly Thr His Val Ala 
    55                  60                  65                  


Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Leu Gly Val Ala 
70                  75                  80                  85  


Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu Gly Ala Asp Gly Ser 
                90                  95                  100     


Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu Trp Ala Ile Ala Asn 
            105                 110                 115         


Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro Ser Gly Ser Ala 
        120                 125                 130             


Ala Leu Lys Ala Ala Val Asp Lys Ala Val Ala Ser Gly Val Val Val 
    135                 140                 145                 


Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly Ser Ser Ser Thr Val 
150                 155                 160                 165 


Gly Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala Val Gly Ala Val Asp 
                170                 175                 180     


Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Val Gly Pro Glu Leu Asp 
            185                 190                 195         


Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr Leu Pro Gly Asn Lys 
        200                 205                 210             


Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Ser Pro His Val Ala Gly 
    215                 220                 225                 


Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Trp Thr Asn Thr Gln 
230                 235                 240                 245 


Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys Leu Gly Asp Ser Phe 
                250                 255                 260     


Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala Ala Ala Gln 
            265                 270                 275 


<210>  3
<211>  1143
<212>  DNA
<213>  Bacillus lentus


<220>
<221>  CDS
<222>  (1)..(1140)

<220>
<221>  sig_peptide
<222>  (1)..(81)

<220>
<221>  mat_peptide
<222>  (334)..(1140)

<400>  3
atg aag  aaa ccg ttg ggg aaa  att gtc gca agc acc  gca cta ctc          45
Met Lys  Lys Pro Leu Gly Lys  Ile Val Ala Ser Thr  Ala Leu Leu            
    -110                 -105                 -100                        

att tct gtt gct ttt agt tca tcg atc gca tcg gct gct gaa gaa gca         93
Ile Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Glu Glu Ala           
    -95                 -90                 -85                           

aaa gaa aaa tat tta att ggc ttt aat gag cag gaa gct gtc agt gag        141
Lys Glu Lys Tyr Leu Ile Gly Phe Asn Glu Gln Glu Ala Val Ser Glu           
-80                 -75                 -70                 -65           

ttt gta gaa caa gta gag gca aat gac gag gtc gcc att ctc tct gag        189
Phe Val Glu Gln Val Glu Ala Asn Asp Glu Val Ala Ile Leu Ser Glu           
                -60                 -55                 -50               

gaa gag gaa gtc gaa att gaa ttg ctt cat gaa ttt gaa acg att cct        237
Glu Glu Glu Val Glu Ile Glu Leu Leu His Glu Phe Glu Thr Ile Pro           
            -45                 -40                 -35                   

gtt tta tcc gtt gag tta agc cca gaa gat gtg gac gcg ctt gaa ctc        285
Val Leu Ser Val Glu Leu Ser Pro Glu Asp Val Asp Ala Leu Glu Leu           
        -30                 -25                 -20                       

gat cca gcg att tct tat att gaa gag gat gca gaa gta acg aca atg        333
Asp Pro Ala Ile Ser Tyr Ile Glu Glu Asp Ala Glu Val Thr Thr Met           
    -15                 -10                 -5              -1            

gcg caa tcg gta cca tgg gga att agc cgt gtg caa gcc cca gct gcc        381
Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala           
1               5                   10                  15                

cat aac cgt gga ttg aca ggt tct ggt gta aaa gtt gct gtc ctc gat        429
His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp           
            20                  25                  30                    

aca ggg ata tcc act cat cca gat cta aat att cgt ggt ggc gca agc        477
Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser           
        35                  40                  45                        

ttt gta cca ggg gaa ccg tcg act caa gat ggg aat ggg cat ggc acg        525
Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr           
    50                  55                  60                            

cat gtg gcc ggg acg atc gct gct tta aac aat tcg att ggc gtt ctt        573
His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu           
65                  70                  75                  80            

ggc gta gct cct agc gct gag cta tac gct gtt aaa gtc cta ggg gcg        621
Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala           
                85                  90                  95                

agc ggt tca ggt tcg gtc agc tcg att gcc caa gga ttg gaa tgg gca        669
Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala           
            100                 105                 110                   

ggg aac aat ggc atg cac gtt gct aat ttg agt tta gga agc cct tcg        717
Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser           
        115                 120                 125                       

cca agt gcc aca ctc gag caa gct gtt aat agc gcg act tct aga ggc        765
Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly           
    130                 135                 140                           

gtt ctt gtt gta gcg gca tct ggg aat tca ggt gca ggc tca atc agc        813
Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser           
145                 150                 155                 160           

tat ccg gcg cgc tat gcg aac gca atg gca gtc gga gct act gat caa        861
Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln           
                165                 170                 175               

aac aac aac cgc gct agc ttt tca cag tat ggc gca ggc ctt gac att        909
Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile           
            180                 185                 190                   

gtc gca ccc ggg gta aac gtg cag agc aca tac cca ggt tca aca tat        957
Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr           
        195                 200                 205                       

gcc agc tta aac ggt aca tcg atg gct act cct cat gtt gca ggt gcg       1005
Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala           
    210                 215                 220                           

gcc gcc ctt gtt aaa caa aag aac cca tct tgg tct aat gta caa att       1053
Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile           
225                 230                 235                 240           

cga aat cat cta aag aat acg gca act agt tta gga agc acg aac ttg       1101
Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu           
                245                 250                 255               

tat gga agc gga ctt gtt aac gca gaa gcg gca acg cgt taa               1143
Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg                       
            260                 265                                       


<210>  4
<211>  380
<212>  PRT
<213>  Bacillus lentus

<400>  4

Met Lys  Lys Pro Leu Gly Lys  Ile Val Ala Ser Thr  Ala Leu Leu 
    -110                 -105                 -100             


Ile Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Glu Glu Ala 
    -95                 -90                 -85                 


Lys Glu Lys Tyr Leu Ile Gly Phe Asn Glu Gln Glu Ala Val Ser Glu 
-80                 -75                 -70                 -65 


Phe Val Glu Gln Val Glu Ala Asn Asp Glu Val Ala Ile Leu Ser Glu 
                -60                 -55                 -50     


Glu Glu Glu Val Glu Ile Glu Leu Leu His Glu Phe Glu Thr Ile Pro 
            -45                 -40                 -35         


Val Leu Ser Val Glu Leu Ser Pro Glu Asp Val Asp Ala Leu Glu Leu 
        -30                 -25                 -20             


Asp Pro Ala Ile Ser Tyr Ile Glu Glu Asp Ala Glu Val Thr Thr Met 
    -15                 -10                 -5              -1  


Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


