                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  EXPRESSION OF NATIVELY SECRETED POLYPEPTIDES WITHOUT SIGNAL 
       PEPTIDE

<130>  12621-WO-PCT

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  1965
<212>  DNA
<213>  Pyrococcus furiosus

<400>  1
atgaaggggc taaaagctct catattagtg attttagttc taggtttggt agtagggagc       60

gtagcggcag ctccagagaa gaaagttgaa caagtaagaa atgttgagaa gaactatggt      120

ctgctaacgc caggactgtt cagaaaaatt caaaaattga atcctaacga ggaaatcagc      180

acagtaattg tatttgaaaa ccatagggaa aaagaaattg cagtaagagt tcttgagtta      240

atgggtgcaa aagttaggta tgtgtaccat attatacccg caatagctgc cgatcttaag      300

gttagagact tactagtcat ctcaggttta acagggggta aagctaagct ttcaggtgtt      360

aggtttatcc aggaagacta caaagttaca gtttcagcag aattagaagg actggatgag      420

tctgcagctc aagttatggc aacttacgtt tggaacttgg gatatgatgg ttctggaatc      480

acaataggaa taattgacac tggaattgac gcttctcatc cagatctcca aggaaaagta      540

attgggtggg tagattttgt caatggtagg agttatccat acgatgacca tggacatgga      600

actcatgtag cttcaatagc agctggtact ggagcagcaa gtaatggcaa gtacaaggga      660

atggctccag gagctaagct ggcgggaatt aaggttctag gtgccgatgg ttctggaagc      720

atatctacta taattaaggg agttgagtgg gccgttgata acaaagataa gtacggaatt      780

aaggtcatta atctttctct tggttcaagc cagagctcag atggtactga cgctctaagt      840

caggctgtta atgcagcgtg ggatgctgga ttagttgttg tggttgccgc tggaaacagt      900

ggacctaaca agtatacaat cggttctcca gcagctgcaa gcaaagttat tacagttgga      960

gccgttgaca agtatgatgt tataacaagc ttctcaagca gagggccaac tgcagacggc     1020

aggcttaagc ctgaggttgt tgctccagga aactggataa ttgctgccag agcaagtgga     1080

actagcatgg gtcaaccaat taatgactat tacacagcag ctcctgggac atcaatggca     1140

actcctcacg tagctggtat tgcagccctc ttgctccaag cacacccgag ctggactcca     1200

gacaaagtaa aaacagccct catagaaact gctgatatcg taaagccaga tgaaatagcc     1260

gatatagcct acggtgcagg tagggttaat gcatacaagg ctataaacta cgataactat     1320

gcaaagctag tgttcactgg atatgttgcc aacaaaggca gccaaactca ccagttcgtt     1380

attagcggag cttcgttcgt aactgccaca ttatactggg acaatgccaa tagcgacctt     1440

gatctttacc tctacgatcc caatggaaac caggttgact actcttacac cgcctactat     1500

ggattcgaaa aggttggtta ttacaaccca actgatggaa catggacaat taaggttgta     1560

agctacagcg gaagtgcaaa ctatcaagta gatgtggtaa gtgatggttc cctttcacag     1620

cctggaagtt caccatctcc acaaccagaa ccaacagtag acgcaaagac gttccaagga     1680

tccgttcact actactatga caggagcgac acctttacaa tgaccgttaa ctctggggct     1740

acaaagatta ctggagacct agtgtttgac acaagctacc atgatcttga cctttacctc     1800

tacgatccta accagaagct tgtagataga tcggagagtt ccaacagcta cgaacacgta     1860

gaatacttaa accccgcccc aggaacctgg tacttcctag tatatgccta ctacacttac     1920

ggttgggctt actacgagct gacggctaaa gtttattatg gctga                     1965


<210>  2
<211>  654
<212>  PRT
<213>  Pyrococcus furiosus


<220>
<221>  mat_peptide
<222>  (133)..(545)
<223>  Mature peptide

<400>  2

Met Lys Gly  Leu Lys Ala Leu Ile  Leu Val Ile Leu Val  Leu Gly 
        -130                 -125                 -120         


Leu Val Val  Gly Ser Val Ala Ala  Ala Pro Glu Lys Lys  Val Glu 
        -115                 -110                 -105         


Gln Val Arg  Asn Val Glu Lys Asn Tyr Gly Leu Leu Thr Pro Gly Leu 
        -100                 -95                 -90             


Phe Arg Lys Ile Gln Lys Leu Asn Pro Asn Glu Glu Ile Ser Thr Val 
    -85                 -80                 -75                 


Ile Val Phe Glu Asn His Arg Glu Lys Glu Ile Ala Val Arg Val Leu 
-70                 -65                 -60                 -55 


Glu Leu Met Gly Ala Lys Val Arg Tyr Val Tyr His Ile Ile Pro Ala 
                -50                 -45                 -40     


Ile Ala Ala Asp Leu Lys Val Arg Asp Leu Leu Val Ile Ser Gly Leu 
            -35                 -30                 -25         


Thr Gly Gly Lys Ala Lys Leu Ser Gly Val Arg Phe Ile Gln Glu Asp 
        -20                 -15                 -10             


Tyr Lys Val Thr Val Ser Ala Glu Leu Glu Gly Leu Asp Glu Ser Ala 
    -5              -1  1               5                   10  


Ala Gln Val Met Ala Thr Tyr Val Trp Asn Leu Gly Tyr Asp Gly Ser 
                15                  20                  25      


Gly Ile Thr Ile Gly Ile Ile Asp Thr Gly Ile Asp Ala Ser His Pro 
            30                  35                  40          


Asp Leu Gln Gly Lys Val Ile Gly Trp Val Asp Phe Val Asn Gly Arg 
        45                  50                  55              


Ser Tyr Pro Tyr Asp Asp His Gly His Gly Thr His Val Ala Ser Ile 
    60                  65                  70                  


Ala Ala Gly Thr Gly Ala Ala Ser Asn Gly Lys Tyr Lys Gly Met Ala 
75                  80                  85                  90  


Pro Gly Ala Lys Leu Ala Gly Ile Lys Val Leu Gly Ala Asp Gly Ser 
                95                  100                 105     


Gly Ser Ile Ser Thr Ile Ile Lys Gly Val Glu Trp Ala Val Asp Asn 
            110                 115                 120         


Lys Asp Lys Tyr Gly Ile Lys Val Ile Asn Leu Ser Leu Gly Ser Ser 
        125                 130                 135             


Gln Ser Ser Asp Gly Thr Asp Ala Leu Ser Gln Ala Val Asn Ala Ala 
    140                 145                 150                 


Trp Asp Ala Gly Leu Val Val Val Val Ala Ala Gly Asn Ser Gly Pro 
155                 160                 165                 170 


Asn Lys Tyr Thr Ile Gly Ser Pro Ala Ala Ala Ser Lys Val Ile Thr 
                175                 180                 185     


Val Gly Ala Val Asp Lys Tyr Asp Val Ile Thr Ser Phe Ser Ser Arg 
            190                 195                 200         


Gly Pro Thr Ala Asp Gly Arg Leu Lys Pro Glu Val Val Ala Pro Gly 
        205                 210                 215             


Asn Trp Ile Ile Ala Ala Arg Ala Ser Gly Thr Ser Met Gly Gln Pro 
    220                 225                 230                 


Ile Asn Asp Tyr Tyr Thr Ala Ala Pro Gly Thr Ser Met Ala Thr Pro 
235                 240                 245                 250 


His Val Ala Gly Ile Ala Ala Leu Leu Leu Gln Ala His Pro Ser Trp 
                255                 260                 265     


Thr Pro Asp Lys Val Lys Thr Ala Leu Ile Glu Thr Ala Asp Ile Val 
            270                 275                 280         


Lys Pro Asp Glu Ile Ala Asp Ile Ala Tyr Gly Ala Gly Arg Val Asn 
        285                 290                 295             


Ala Tyr Lys Ala Ile Asn Tyr Asp Asn Tyr Ala Lys Leu Val Phe Thr 
    300                 305                 310                 


Gly Tyr Val Ala Asn Lys Gly Ser Gln Thr His Gln Phe Val Ile Ser 
315                 320                 325                 330 


Gly Ala Ser Phe Val Thr Ala Thr Leu Tyr Trp Asp Asn Ala Asn Ser 
                335                 340                 345     


Asp Leu Asp Leu Tyr Leu Tyr Asp Pro Asn Gly Asn Gln Val Asp Tyr 
            350                 355                 360         


Ser Tyr Thr Ala Tyr Tyr Gly Phe Glu Lys Val Gly Tyr Tyr Asn Pro 
        365                 370                 375             


Thr Asp Gly Thr Trp Thr Ile Lys Val Val Ser Tyr Ser Gly Ser Ala 
    380                 385                 390                 


Asn Tyr Gln Val Asp Val Val Ser Asp Gly Ser Leu Ser Gln Pro Gly 
395                 400                 405                 410 


Ser Ser Pro Ser Pro Gln Pro Glu Pro Thr Val Asp Ala Lys Thr Phe 
                415                 420                 425     


Gln Gly Ser Val His Tyr Tyr Tyr Asp Arg Ser Asp Thr Phe Thr Met 
            430                 435                 440         


Thr Val Asn Ser Gly Ala Thr Lys Ile Thr Gly Asp Leu Val Phe Asp 
        445                 450                 455             


Thr Ser Tyr His Asp Leu Asp Leu Tyr Leu Tyr Asp Pro Asn Gln Lys 
    460                 465                 470                 


Leu Val Asp Arg Ser Glu Ser Ser Asn Ser Tyr Glu His Val Glu Tyr 
475                 480                 485                 490 


Leu Asn Pro Ala Pro Gly Thr Trp Tyr Phe Leu Val Tyr Ala Tyr Tyr 
                495                 500                 505     


Thr Tyr Gly Trp Ala Tyr Tyr Glu Leu Thr Ala Lys Val Tyr Tyr Gly 
            510                 515                 520         


<210>  3
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer

<400>  3
aaaggagagg ataaagaatg gcacctgaga agaaa                                  35


<210>  4
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer

<400>  4
gcgttttttt attgattaac gcgtttatgg tgatgagcca ggc                         43


<210>  5
<211>  1572
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Final intended PfuS-encoding DNA fragment.

<400>  5
atggcacctg agaagaaagt tgagcaagtt cgcaacgtag agaaaaacta cggtcttctt       60

acaccaggcc ttttccgcaa aatccaaaaa cttaacccta acgaggagat cagcactgta      120

atcgtttttg agaaccatcg cgagaaggag atcgctgttc gcgttcttga gcttatgggt      180

gcgaaggtac gctacgttta ccatatcatt ccggctattg cggctgacct taaggttcgc      240

gaccttcttg ttatctctgg tcttactggt ggcaaagcga aactttcagg cgttcgcttc      300

atccaagagg actacaaagt tactgtatct gctgagcttg agggacttga cgagtcagcg      360

gcacaagtaa tggcaacata cgtatggaac cttggctacg acggttctgg catcactatc      420

ggcatcatcg acacgggcat cgacgcttca caccctgacc ttcaaggtaa ggtaatcggt      480

tgggttgact tcgttaatgg tcgctcttat ccgtatgacg accatggcca cggtacacac      540

gtagcatcta tcgcagctgg cactggcgca gcttctaacg gcaagtacaa aggcatggca      600

cctggtgcga aacttgctgg tatcaaagta cttggcgcag acggctctgg ctcaatcagc      660

acaatcatca aaggcgttga gtgggctgtt gacaacaagg acaaatacgg tatcaaagtt      720

atcaaccttt ctcttggctc ttctcaaagc tctgacggca cagacgcgct ttcacaagct      780

gttaacgctg cttgggacgc tggtcttgta gttgttgttg ctgctggtaa cagcggtcca      840

aacaaataca ctatcggctc accggcagct gcgtctaaag taatcacagt tggagctgta      900

gacaaatacg acgttatcac ttctttctca tctcgtggcc ctactgcaga tggtcgcctt      960

aaaccagagg ttgtagcacc aggcaactgg atcatcgcag ctcgcgcttc tggcacatca     1020

atgggccaac caatcaacga ctactatact gctgcgccag gaacttctat ggctactcca     1080

cacgtagcag gtatcgctgc acttcttctt caagctcacc cttcttggac gcctgacaaa     1140

gtaaagactg cacttatcga gactgctgac atcgttaaac ctgacgagat cgcagacatc     1200

gcttatggtg ctggtcgcgt taatgcgtac aaggctatca actatgacaa ctatgctaaa     1260

cttgtattca cgggctacgt agctaacaaa ggctctcaaa cgcaccaatt tgttatctct     1320

ggcgcaagct tcgttactgc tactctttac tgggacaacg ctaactctga ccttgacctt     1380

tacttatacg acccaaacgg caaccaggtt gactattctt atactgcata ctacggcttt     1440

gagaaggttg gctattacaa ccctactgac ggcacatgga caatcaaagt agtaagctat     1500

tcaggatcag ctaactacca agtagacgta gtttctgacg gttctcttag ccagcctggc     1560

tcatcaccat aa                                                         1572


<210>  6
<211>  523
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Intended final PfuS amino acid sequence.


<220>
<221>  PROPEP
<222>  (1)..(110)

<220>
<221>  mat_peptide
<222>  (111)..(523)
<223>  Mature peptide

<400>  6

Met  Ala Pro Glu Lys Lys  Val Glu Gln Val Arg  Asn Val Glu Lys 
-110                 -105                 -100                 


Asn Tyr Gly Leu Leu Thr Pro Gly Leu Phe Arg Lys Ile Gln Lys Leu 
-95                 -90                 -85                 -80 


Asn Pro Asn Glu Glu Ile Ser Thr Val Ile Val Phe Glu Asn His Arg 
                -75                 -70                 -65     


Glu Lys Glu Ile Ala Val Arg Val Leu Glu Leu Met Gly Ala Lys Val 
            -60                 -55                 -50         


Arg Tyr Val Tyr His Ile Ile Pro Ala Ile Ala Ala Asp Leu Lys Val 
        -45                 -40                 -35             


Arg Asp Leu Leu Val Ile Ser Gly Leu Thr Gly Gly Lys Ala Lys Leu 
    -30                 -25                 -20                 


Ser Gly Val Arg Phe Ile Gln Glu Asp Tyr Lys Val Thr Val Ser Ala 
-15                 -10                 -5              -1  1   


Glu Leu Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr Tyr 
            5                   10                  15          


Val Trp Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile Ile 
        20                  25                  30              


Asp Thr Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val Ile 
    35                  40                  45                  


Gly Trp Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp His 
50                  55                  60                  65  


Gly His Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala Ala 
                70                  75                  80      


Ser Asn Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala Gly 
            85                  90                  95          


Ile Lys Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile Ile 
        100                 105                 110             


Lys Gly Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile Lys 
    115                 120                 125                 


Val Ile Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr Asp 
130                 135                 140                 145 


Ala Leu Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val Val 
                150                 155                 160     


Val Val Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly Ser 
            165                 170                 175         


Pro Ala Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys Tyr 
        180                 185                 190             


Asp Val Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly Arg 
    195                 200                 205                 


Leu Lys Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala Arg 
210                 215                 220                 225 


Ala Ser Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr Ala 
                230                 235                 240     


Ala Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala Ala 
            245                 250                 255         


Leu Leu Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys Thr 
        260                 265                 270             


Ala Leu Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala Asp 
    275                 280                 285                 


Ile Ala Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn Tyr 
290                 295                 300                 305 


Asp Asn Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys Gly 
                310                 315                 320     


Ser Gln Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr Ala 
            325                 330                 335         


Thr Leu Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu Tyr 
        340                 345                 350             


Asp Pro Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr Gly 
    355                 360                 365                 


Phe Glu Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr Ile 
370                 375                 380                 385 


Lys Val Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val Val 
                390                 395                 400     


Ser Asp Gly Ser Leu Ser Gln Pro Gly Ser Ser Pro 
            405                 410             


<210>  7
<211>  1572
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Actual final PfuS-encoding DNA fragment.

<400>  7
atggcacctg agaagaaagt tgagcaagtt cgcaacgtag agaaaaacta cggtcttctt       60

acaccaggcc ttttccgcaa aatccaaaaa cttaacccta acgaggagat cagcactgta      120

atcgtttttg agaaccatcg cgagaaggag atcgctgttc gcgttcttga gcttatgggt      180

gcgaaggtac gctacgttta ccatatcatt ccggctattg cggctgacct taaggttcgc      240

gaccttcttg ttatctctgg tcttactggt ggcaaagcga aactttcagg cgttcgcttc      300

atccaagagg actacaaagt tactgtatct gctgagcttg agggacttga cgagtcagcg      360

gcacaagtaa tggcaacata cgtatggaac cttggctacg acggttctgg catcactatc      420

ggcatcatcg acacgggcat cgacgcttca caccctgacc ttcaaggtaa ggtaatcggt      480

tgggttgact tcgttaatgg tcgctcttat ccgtatgacg accatggcca cggtacacac      540

gtagcatcta tcgcagctgg cactggcgca gcttctaacg gcaagtacaa aggcatggca      600

cctggtgcga aacttgctgg tatcaaagta cttggcgcag acggctctgg ctcaatcagc      660

acaatcatca aaggcgttga gtgggctgtt gacaacaagg acaaatacgg tatcaaagtt      720

atcaaccttt ctcttggctc ttctcaaagc tctgacggca cagacgcgct ttcacaagct      780

gttaacgctg cttgggacgc tggtcttgta gttgttgttg ctgctggtaa cagcggtcca      840

aacaaataca ctatcggctc accggcagct gcgtctaaag taatcacagt tggagctgta      900

gacaaatacg acgttatcac ttctttctca tctcgtggcc ctactgcaga tggtcgcctt      960

aaaccagagg ttgtagcacc aggcaactgg atcatcgcag ctcgcgcttc tggcacatca     1020

atgggccaac caatcaacga ctactatact gctgcgccag gaacttctat ggctactcca     1080

cacgtagcag gtatcgctgc acttcttctt caagctcacc cttcttggac gcctgacaaa     1140

gtaaagactg cacttatcga gactgctgac atcgttaaac ctgacgagat cgcagacatc     1200

gcttatggtg ctggtcgcgt taatgcgtac aaggctatca actatgacaa ctatgctaaa     1260

cttgtattca cgggctacgt agctaacaaa ggctctcaaa cgcaccaatt tgttatctct     1320

ggcgcaagct tcgttactgc tactctttac tgggacaacg ctaactctga ccttgacctt     1380

tacttatacg acccaaacgg caaccaggtt gactattctt atactgcata ctacgacttt     1440

gagaaggttg gctattacaa ccctactgac ggcacatgga caatcaaagt agtaagctat     1500

tcaggatcag ctaactacca agtagacgta gtttctgacg gttctcttag ccagcctggc     1560

tcatcaccat aa                                                         1572


<210>  8
<211>  523
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Actual variant PfuS amino acid sequence.


<220>
<221>  PROPEP
<222>  (1)..(110)

<220>
<221>  mat_peptide
<222>  (111)..(523)

<220>
<221>  VARIANT
<222>  (369)..(369)
<223>  Gly to Asp (G369D) mutation.

<400>  8

Met  Ala Pro Glu Lys Lys  Val Glu Gln Val Arg  Asn Val Glu Lys 
-110                 -105                 -100                 


Asn Tyr Gly Leu Leu Thr Pro Gly Leu Phe Arg Lys Ile Gln Lys Leu 
-95                 -90                 -85                 -80 


Asn Pro Asn Glu Glu Ile Ser Thr Val Ile Val Phe Glu Asn His Arg 
                -75                 -70                 -65     


Glu Lys Glu Ile Ala Val Arg Val Leu Glu Leu Met Gly Ala Lys Val 
            -60                 -55                 -50         


Arg Tyr Val Tyr His Ile Ile Pro Ala Ile Ala Ala Asp Leu Lys Val 
        -45                 -40                 -35             


Arg Asp Leu Leu Val Ile Ser Gly Leu Thr Gly Gly Lys Ala Lys Leu 
    -30                 -25                 -20                 


Ser Gly Val Arg Phe Ile Gln Glu Asp Tyr Lys Val Thr Val Ser Ala 
-15                 -10                 -5              -1  1   


Glu Leu Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr Tyr 
            5                   10                  15          


Val Trp Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile Ile 
        20                  25                  30              


Asp Thr Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val Ile 
    35                  40                  45                  


Gly Trp Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp His 
50                  55                  60                  65  


Gly His Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala Ala 
                70                  75                  80      


Ser Asn Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala Gly 
            85                  90                  95          


Ile Lys Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile Ile 
        100                 105                 110             


Lys Gly Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile Lys 
    115                 120                 125                 


Val Ile Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr Asp 
130                 135                 140                 145 


Ala Leu Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val Val 
                150                 155                 160     


Val Val Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly Ser 
            165                 170                 175         


Pro Ala Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys Tyr 
        180                 185                 190             


Asp Val Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly Arg 
    195                 200                 205                 


Leu Lys Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala Arg 
210                 215                 220                 225 


Ala Ser Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr Ala 
                230                 235                 240     


Ala Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala Ala 
            245                 250                 255         


Leu Leu Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys Thr 
        260                 265                 270             


Ala Leu Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala Asp 
    275                 280                 285                 


Ile Ala Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn Tyr 
290                 295                 300                 305 


Asp Asn Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys Gly 
                310                 315                 320     


Ser Gln Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr Ala 
            325                 330                 335         


Thr Leu Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu Tyr 
        340                 345                 350             


Asp Pro Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr Asp 
    355                 360                 365                 


Phe Glu Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr Ile 
370                 375                 380                 385 


Lys Val Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val Val 
                390                 395                 400     


Ser Asp Gly Ser Leu Ser Gln Pro Gly Ser Ser Pro 
            405                 410             


<210>  9
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  9
tcatcgatcg catcggctgc acctgagaag aaagttg                                37


<210>  10
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  10
ccaaggccgg ttttttatgt tttatggtga tgagccaggc                             40


<210>  11
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA encoding the B. clausii aprH protease signal peptide.


<220>
<221>  sig_peptide
<222>  (1)..(81)
<223>  Encodes the signal peptide

<220>
<221>  misc_feature
<222>  (82)..(90)
<223>  DNA left over from the cloning process.

<400>  11
atgaaaaaac cgctggggaa aattgtcgca agcaccgcac tactcatttc tgttgctttt       60

agttcatcga tcgcatcggc tgcacctagg                                        90


<210>  12
<211>  1659
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA fragment encoding the PfuS protease fused N-terminally to the
       AprH signal peptide.


<220>
<221>  CDS
<222>  (1)..(1656)
<223>  Encodes PfuS protease fused N-terminally to the AprH signal 
       peptide.

<220>
<221>  sig_peptide
<222>  (1)..(81)
<223>  AprH signal peptide

<220>
<221>  misc_feature
<222>  (82)..(417)
<223>  Propeptide

<220>
<221>  mat_peptide
<222>  (418)..(1656)

<400>  12
atg aaa aaa ccg ctg  ggg aaa att gtc gca  agc acc gca cta ctc           45
Met Lys Lys Pro Leu  Gly Lys Ile Val Ala  Ser Thr Ala Leu Leu             
                -135                 -130                 -125            

att tct gtt gct ttt  agt tca tcg atc gca  tcg gct gca cct agg           90
Ile Ser Val Ala Phe  Ser Ser Ser Ile Ala  Ser Ala Ala Pro Arg             
                -120                 -115                 -110            

gca cct gag aag aaa  gtt gag caa gtt cgc  aac gta gag aaa aac tac      138
Ala Pro Glu Lys Lys  Val Glu Gln Val Arg  Asn Val Glu Lys Asn Tyr         
                -105                 -100                 -95             

ggt ctt ctt aca cca ggc ctt ttc cgc aaa atc caa aaa ctt aac cct        186
Gly Leu Leu Thr Pro Gly Leu Phe Arg Lys Ile Gln Lys Leu Asn Pro           
            -90                 -85                 -80                   

aac gag gag atc agc act gta atc gtt ttt gag aac cat cgc gag aag        234
Asn Glu Glu Ile Ser Thr Val Ile Val Phe Glu Asn His Arg Glu Lys           
        -75                 -70                 -65                       

gag atc gct gtt cgc gtt ctt gag ctt atg ggt gcg aag gta cgc tac        282
Glu Ile Ala Val Arg Val Leu Glu Leu Met Gly Ala Lys Val Arg Tyr           
    -60                 -55                 -50                           

gtt tac cat atc att ccg gct att gcg gct gac ctt aag gtt cgc gac        330
Val Tyr His Ile Ile Pro Ala Ile Ala Ala Asp Leu Lys Val Arg Asp           
-45                 -40                 -35                 -30           

ctt ctt gtt atc tct ggt ctt act ggt ggc aaa gcg aaa ctt tca ggc        378
Leu Leu Val Ile Ser Gly Leu Thr Gly Gly Lys Ala Lys Leu Ser Gly           
                -25                 -20                 -15               

gtt cgc ttc atc caa gag gac tac aaa gtt act gta tct gct gag ctt        426
Val Arg Phe Ile Gln Glu Asp Tyr Lys Val Thr Val Ser Ala Glu Leu           
            -10                 -5              -1  1                     

gag gga ctt gac gag tca gcg gca caa gta atg gca aca tac gta tgg        474
Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr Tyr Val Trp           
    5                   10                  15                            

aac ctt ggc tac gac ggt tct ggc atc act atc ggc atc atc gac acg        522
Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile Ile Asp Thr           
20                  25                  30                  35            

ggc atc gac gct tca cac cct gac ctt caa ggt aag gta atc ggt tgg        570
Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val Ile Gly Trp           
                40                  45                  50                

gtt gac ttc gtt aat ggt cgc tct tat ccg tat gac gac cat ggc cac        618
Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp His Gly His           
            55                  60                  65                    

ggt aca cac gta gca tct atc gca gct ggc act ggc gca gct tct aac        666
Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala Ala Ser Asn           
        70                  75                  80                        

ggc aag tac aaa ggc atg gca cct ggt gcg aaa ctt gct ggt atc aaa        714
Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala Gly Ile Lys           
    85                  90                  95                            

gta ctt ggc gca gac ggc tct ggc tca atc agc aca atc atc aaa ggc        762
Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile Ile Lys Gly           
100                 105                 110                 115           

gtt gag tgg gct gtt gac aac aag gac aaa tac ggt atc aaa gtt atc        810
Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile Lys Val Ile           
                120                 125                 130               

aac ctt tct ctt ggc tct tct caa agc tct gac ggc aca gac gcg ctt        858
Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr Asp Ala Leu           
            135                 140                 145                   

tca caa gct gtt aac gct gct tgg gac gct ggt ctt gta gtt gtt gtt        906
Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val Val Val Val           
        150                 155                 160                       

gct gct ggt aac agc ggt cca aac aaa tac act atc ggc tca ccg gca        954
Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly Ser Pro Ala           
    165                 170                 175                           

gct gcg tct aaa gta atc aca gtt gga gct gta gac aaa tac gac gtt       1002
Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys Tyr Asp Val           
180                 185                 190                 195           

atc act tct ttc tca tct cgt ggc cct act gca gat ggt cgc ctt aaa       1050
Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly Arg Leu Lys           
                200                 205                 210               

cca gag gtt gta gca cca ggc aac tgg atc atc gca gct cgc gct tct       1098
Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala Arg Ala Ser           
            215                 220                 225                   

ggc aca tca atg ggc caa cca atc aac gac tac tat act gct gcg cca       1146
Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr Ala Ala Pro           
        230                 235                 240                       

gga act tct atg gct act cca cac gta gca ggt atc gct gca ctt ctt       1194
Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala Ala Leu Leu           
    245                 250                 255                           

ctt caa gct cac cct tct tgg acg cct gac aaa gta aag act gca ctt       1242
Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys Thr Ala Leu           
260                 265                 270                 275           

atc gag act gct gac atc gtt aaa cct gac gag atc gca gac atc gct       1290
Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala Asp Ile Ala           
                280                 285                 290               

tat ggt gct ggt cgc gtt aat gcg tac aag gct atc aac tat gac aac       1338
Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn Tyr Asp Asn           
            295                 300                 305                   

tat gct aaa ctt gta ttc acg ggc tac gta gct aac aaa ggc tct caa       1386
Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys Gly Ser Gln           
        310                 315                 320                       

acg cac caa ttt gtt atc tct ggc gca agc ttc gtt act gct act ctt       1434
Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr Ala Thr Leu           
    325                 330                 335                           

tac tgg gac aac gct aac tct gac ctt gac ctt tac tta tac gac cca       1482
Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu Tyr Asp Pro           
340                 345                 350                 355           

aac ggc aac cag gtt gac tat tct tat act gca tac tac ggc ttt gag       1530
Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr Gly Phe Glu           
                360                 365                 370               

aag gtt ggc tat tac aac cct act gac ggc aca tgg aca atc aaa gta       1578
Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr Ile Lys Val           
            375                 380                 385                   

gta agc tat tca gga tca gct aac tac caa gta gac gta gtt tct gac       1626
Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val Val Ser Asp           
        390                 395                 400                       

ggt tct ctt agc cag cct ggc tca tca cca taa                           1659
Gly Ser Leu Ser Gln Pro Gly Ser Ser Pro                                   
    405                 410                                               


<210>  13
<211>  552
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  13

Met Lys Lys Pro Leu  Gly Lys Ile Val Ala  Ser Thr Ala Leu Leu  
                -135                 -130                 -125 


Ile Ser Val Ala Phe  Ser Ser Ser Ile Ala  Ser Ala Ala Pro Arg  
                -120                 -115                 -110 


Ala Pro Glu Lys Lys  Val Glu Gln Val Arg  Asn Val Glu Lys Asn Tyr 
                -105                 -100                 -95     


Gly Leu Leu Thr Pro Gly Leu Phe Arg Lys Ile Gln Lys Leu Asn Pro 
            -90                 -85                 -80         


Asn Glu Glu Ile Ser Thr Val Ile Val Phe Glu Asn His Arg Glu Lys 
        -75                 -70                 -65             


Glu Ile Ala Val Arg Val Leu Glu Leu Met Gly Ala Lys Val Arg Tyr 
    -60                 -55                 -50                 


Val Tyr His Ile Ile Pro Ala Ile Ala Ala Asp Leu Lys Val Arg Asp 
-45                 -40                 -35                 -30 


Leu Leu Val Ile Ser Gly Leu Thr Gly Gly Lys Ala Lys Leu Ser Gly 
                -25                 -20                 -15     


Val Arg Phe Ile Gln Glu Asp Tyr Lys Val Thr Val Ser Ala Glu Leu 
            -10                 -5              -1  1           


Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr Tyr Val Trp 
    5                   10                  15                  


Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile Ile Asp Thr 
20                  25                  30                  35  


Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val Ile Gly Trp 
                40                  45                  50      


Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp His Gly His 
            55                  60                  65          


Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala Ala Ser Asn 
        70                  75                  80              


Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala Gly Ile Lys 
    85                  90                  95                  


Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile Ile Lys Gly 
100                 105                 110                 115 


Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile Lys Val Ile 
                120                 125                 130     


Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr Asp Ala Leu 
            135                 140                 145         


Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val Val Val Val 
        150                 155                 160             


Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly Ser Pro Ala 
    165                 170                 175                 


Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys Tyr Asp Val 
180                 185                 190                 195 


Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly Arg Leu Lys 
                200                 205                 210     


Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala Arg Ala Ser 
            215                 220                 225         


Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr Ala Ala Pro 
        230                 235                 240             


Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala Ala Leu Leu 
    245                 250                 255                 


Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys Thr Ala Leu 
260                 265                 270                 275 


Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala Asp Ile Ala 
                280                 285                 290     


Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn Tyr Asp Asn 
            295                 300                 305         


Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys Gly Ser Gln 
        310                 315                 320             


Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr Ala Thr Leu 
    325                 330                 335                 


Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu Tyr Asp Pro 
340                 345                 350                 355 


Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr Gly Phe Glu 
                360                 365                 370     


Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr Ile Lys Val 
            375                 380                 385         


Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val Val Ser Asp 
        390                 395                 400             


Gly Ser Leu Ser Gln Pro Gly Ser Ser Pro 
    405                 410             


<210>  14
<211>  612
<212>  DNA
<213>  artificial sequence

<220>
<223>  A codon-optimized gene encoding the mature Dictyoglomus 
       thermophilum xylanase (xylanase-domain only) with an N-terminal 
       methionine added.


<220>
<221>  CDS
<222>  (1)..(612)
<223>  A codon-optimized gene encoding the mature Dictyoglomus 
       thermophilum xylanase (xylanase-domain only). An ATG 
       translational start codon has been added to the 5'-end of the 
       CDS.

<400>  14
atg cag aca tca atc aca ctt aca tct aac gca tca ggc aca ttc gac         48
Met Gln Thr Ser Ile Thr Leu Thr Ser Asn Ala Ser Gly Thr Phe Asp           
1               5                   10                  15                

ggc tat tac tac gag ctt tgg aag gac aca ggc aac acg act atg act         96
Gly Tyr Tyr Tyr Glu Leu Trp Lys Asp Thr Gly Asn Thr Thr Met Thr           
            20                  25                  30                    

gta tac act caa ggt cgc ttc tca tgc cag tgg tct aac atc aac aac        144
Val Tyr Thr Gln Gly Arg Phe Ser Cys Gln Trp Ser Asn Ile Asn Asn           
        35                  40                  45                        

gcg ctt ttc cgc acg ggc aag aag tac aac cag aac tgg caa tct ctt        192
Ala Leu Phe Arg Thr Gly Lys Lys Tyr Asn Gln Asn Trp Gln Ser Leu           
    50                  55                  60                            

ggc act atc cgc atc act tat tct gcg aca tac aac ccg aac ggc aac        240
Gly Thr Ile Arg Ile Thr Tyr Ser Ala Thr Tyr Asn Pro Asn Gly Asn           
65                  70                  75                  80            

tct tac ctt tgt atc tac ggc tgg tct acg aac ccg ctt gtt gag ttc        288
Ser Tyr Leu Cys Ile Tyr Gly Trp Ser Thr Asn Pro Leu Val Glu Phe           
                85                  90                  95                

tac atc gta gag tct tgg ggc aac tgg cgt cct cct ggc gca aca tct        336
Tyr Ile Val Glu Ser Trp Gly Asn Trp Arg Pro Pro Gly Ala Thr Ser           
            100                 105                 110                   

ctt ggc cag gtt aca atc gat ggt ggc aca tat gac atc tac cgc act        384
Leu Gly Gln Val Thr Ile Asp Gly Gly Thr Tyr Asp Ile Tyr Arg Thr           
        115                 120                 125                       

act cgc gtt aac cag cct agc atc gtt ggc aca gct act ttc gac caa        432
Thr Arg Val Asn Gln Pro Ser Ile Val Gly Thr Ala Thr Phe Asp Gln           
    130                 135                 140                           

tac tgg agc gtt cgc act agc aag cgc aca tct ggc aca gtt acg gtt        480
Tyr Trp Ser Val Arg Thr Ser Lys Arg Thr Ser Gly Thr Val Thr Val           
145                 150                 155                 160           

acg gac cac ttt cgc gca tgg gca aat cgt ggc ctt aac ctt ggc aca        528
Thr Asp His Phe Arg Ala Trp Ala Asn Arg Gly Leu Asn Leu Gly Thr           
                165                 170                 175               

atc gac caa atc aca ctt tgt gtt gag ggc tac cag tct tct ggc agc        576
Ile Asp Gln Ile Thr Leu Cys Val Glu Gly Tyr Gln Ser Ser Gly Ser           
            180                 185                 190                   

gca aac atc act caa aac act ttc tct cag ggc agc                        612
Ala Asn Ile Thr Gln Asn Thr Phe Ser Gln Gly Ser                           
        195                 200                                           


<210>  15
<211>  204
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  15

Met Gln Thr Ser Ile Thr Leu Thr Ser Asn Ala Ser Gly Thr Phe Asp 
1               5                   10                  15      


Gly Tyr Tyr Tyr Glu Leu Trp Lys Asp Thr Gly Asn Thr Thr Met Thr 
            20                  25                  30          


Val Tyr Thr Gln Gly Arg Phe Ser Cys Gln Trp Ser Asn Ile Asn Asn 
        35                  40                  45              


Ala Leu Phe Arg Thr Gly Lys Lys Tyr Asn Gln Asn Trp Gln Ser Leu 
    50                  55                  60                  


Gly Thr Ile Arg Ile Thr Tyr Ser Ala Thr Tyr Asn Pro Asn Gly Asn 
65                  70                  75                  80  


Ser Tyr Leu Cys Ile Tyr Gly Trp Ser Thr Asn Pro Leu Val Glu Phe 
                85                  90                  95      


Tyr Ile Val Glu Ser Trp Gly Asn Trp Arg Pro Pro Gly Ala Thr Ser 
            100                 105                 110         


Leu Gly Gln Val Thr Ile Asp Gly Gly Thr Tyr Asp Ile Tyr Arg Thr 
        115                 120                 125             


Thr Arg Val Asn Gln Pro Ser Ile Val Gly Thr Ala Thr Phe Asp Gln 
    130                 135                 140                 


Tyr Trp Ser Val Arg Thr Ser Lys Arg Thr Ser Gly Thr Val Thr Val 
145                 150                 155                 160 


Thr Asp His Phe Arg Ala Trp Ala Asn Arg Gly Leu Asn Leu Gly Thr 
                165                 170                 175     


Ile Asp Gln Ile Thr Leu Cys Val Glu Gly Tyr Gln Ser Ser Gly Ser 
            180                 185                 190         


Ala Asn Ile Thr Gln Asn Thr Phe Ser Gln Gly Ser 
        195                 200                 


<210>  16
<211>  8623
<212>  DNA
<213>  artificial sequence

<220>
<223>  Plasmid pJA4156 (see Figure 1; example 4).

<400>  16
ggtaccagat ctaatatctt tgaattgtaa cgcccctcaa aagtaagaac tacaaaaaaa       60

gaatacgtta tatagaaata tgtttgaacc ttcttcagat tacaaatata ttcggacgga      120

ctctacctca aatgcttatc taactataga atgacataca agcacaacct tgaaaatttg      180

aaaatataac taccaatgaa cttgttcatg tgaattatcg ctgtatttaa ttttctcaat      240

tcaatatata atatgccaat acattgttac aagtagaaat taagacaccc ttgatagcct      300

tactatacct aacatgatgt agtattaaat gaatatgtaa atatatttat gataagaagc      360

gacttattta taatcattac atatttttct attggaatga ttaagattcc aatagaatag      420

tgtataaatt atttatcttg aaaggaggga tgcctaaaaa cgaagaacat taaaaacata      480

tatttgcacc gtctaatgga tagaaaggag gtgatccagc cgcaccttat gaaaaatcat      540

tttatcagtt tgaaaattat gtattatgga gctctataaa aatgaggagg gaaccgaatg      600

cagacatcaa tcacacttac atctaacgca tcaggcacat tcgacggcta ttactacgag      660

ctttggaagg acacaggcaa cacgactatg actgtataca ctcaaggtcg cttctcatgc      720

cagtggtcta acatcaacaa cgcgcttttc cgcacgggca agaagtacaa ccagaactgg      780

caatctcttg gcactatccg catcacttat tctgcgacat acaacccgaa cggcaactct      840

tacctttgta tctacggctg gtctacgaac ccgcttgttg agttctacat cgtagagtct      900

tggggcaact ggcgtcctcc tggcgcaaca tctcttggcc aggttacaat cgatggtggc      960

acatatgaca tctaccgcac tactcgcgtt aaccagccta gcatcgttgg cacagctact     1020

ttcgaccaat actggagcgt tcgcactagc aagcgcacat ctggcacagt tacggttacg     1080

gaccactttc gcgcatgggc aaatcgtggc cttaaccttg gcacaatcga ccaaatcaca     1140

ctttgtgttg agggctacca gtcttctggc agcgcaaaca tcactcaaaa cactttctct     1200

cagggcagct aacgcgtagg gcccgcggct agcggccgcg tcgactagaa gagcagagag     1260

gacggatttc ctgaaggaaa tccgtttttt tattttgccc gtcttataaa tttcgttgtc     1320

caactcgctt aattgcgagt ttttatttcg tttatttcaa ttaaggtaac taaagatcct     1380

ctagagtcga ttatgtcttt tgcgcagtcg gcttaaacca gttttcgctg gtgcgaaaaa     1440

agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt     1500

tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa     1560

tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat     1620

aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc     1680

atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg     1740

atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat     1800

agtcgcattc aattcttctg taattatttt tatctgttca taagatttat taccctcata     1860

catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc     1920

atgtaatgga gacactacaa attgaatgtg taactctttt aaatactcta accactcggc     1980

ttttgctgat tctggatata aaacaaatgt ccaattacgt cctcttgaat ttttcttgtt     2040

ttcagtttct tttattacat tttcgctcat gatataataa cggtgctaat acacttaaca     2100

aaatttagtc atagataggc agcatgccag tgctgtctat ctttttttgt ttaaaatgca     2160

ccgtattcct cctttgcata tttttttatt agaataccgg ttgcatctga tttgctaata     2220

ttatattttt ctttgattct atttaatatc tcattttctt ctgttgtaag tcttaaagta     2280

acagcaactt ttttctcttc ttttctatct acaaccatca ctgtacctcc caacatctgt     2340

ttttttcact ttaacataaa aaacaacctt ttaacattaa aaacccaata tttatttatt     2400

tgtttggaca atggacaatg gacacctagg ggggaggtcg tagtaccccc ctatgttttc     2460

tcccctaaat aaccccaaaa atctaagaaa aaaagacctc aaaaaggtct ttaattaaca     2520

tctcaaattt cgcatttatt ccaatttcct ttttgcgtgt gatgcgctgc gtccattaaa     2580

aatcctagag ctttgaaacc gaaagttaat agctgtcgct actactttcg cttacgctct     2640

aagtatattt taaggactgt cacacgcaaa aagttttctc ggcataaaag tacctctaca     2700

tctctaaatc gtctgtacgc tgtttctcac gctttctatc gaccttctgg acattatcct     2760

gtacaacatc cataaactgt cccacacgct caaatttgga atcattaaag aatttctctt     2820

taagcctatt aaaccctttc tcaaacccag ggaaattcgc cctcgcagca cgatataaag     2880

tcactgtact agcttgaaat ttctctgata cattcaactg ctcattcaaa ctatcattct     2940

ctcgctttaa tttattaacc tctttacttt tttcgtgata cccctctttc catgtattca     3000

ctacttcttt caaactctct ctacgttttt ttaattcttg attttctgtg taatagtctg     3060

tgctcttaat attttcgtaa tcatcaacaa tccgttctgc agaagagatt gtttcttgca     3120

ggcgttcaaa ttcatcagca gttaatatct ttctaccagt ctcttcacgt ccagagaaca     3180

aacctgtacg ctcattttca taatcaaagg gtttcgtaga cctcatatgc tctattccac     3240

tctgtaactg cttatttgcc ttctgtaact catccttaac ttcttgcagt tcctgtttat     3300

gaaatacagt atctttcttg tactgatcca tcgctttatg ttctcgttct gtaacctctt     3360

tggacgtgcc tctttcaagt tcataacctt tctcattcac atactcatta aatctatctt     3420

gtaattgagt aaagtctttc ttgttgccta actgttcttt tgcagacaat ctcccgtcct     3480

ctgttaaagg gacaaaacca aagtgcatat gtgggactct ttcatccaga tggacagtcg     3540

catacagcat attttcctta ccgtattcat tttctagaaa ctccaagcta tctttaaaaa     3600

atcgttctat ttcttctccg cttaaatcat caaagaaatc tttatcactt gtaaccagtc     3660

cgtccacatg tcgaattgca tctgaccgaa ttttacgttt ccctgaataa ttctcatcaa     3720

tcgtttcatc aattttatct ttatacttta tattttgtgc gttaatcaaa tcataatttt     3780

tatatgtttc ctcatgattt atgtctttat tattatagtt tttattctct ctttgattat     3840

gtctttgtat cccgtttgta ttacttgatc ctttaactct ggcaaccctc aaaattgaat     3900

gagacatgct acacctccgg ataataaata tatataaacg tatatagatt tcataaagtc     3960

taacacacta gacttattta cttcgtaatt aagtcgttaa accgtgtgct ctacgaccaa     4020

aactataaaa cctttaagaa ctttcttttt ttacaagaaa aaagaaatta gataaatctc     4080

tcatatcttt tattcaataa tcgcatccga ttgcagtata aatttaacga tcactcatca     4140

tgttcatatt tatcagagct cgtgctataa ttatactaat tttataagga ggaaaaaata     4200

tgggcatttt tagtattttt gtaatcagca cagttcatta tcaaccaaac aaaaaataag     4260

tggttataat gaatcgttaa taagcaaaat tcatataacc aaattaaaga gggttataat     4320

gaacgagaaa aatataaaac acagtcaaaa ctttattact tcaaaacata atatagataa     4380

aataatgaca aatataagat taaatgaaca tgataatatc tttgaaatcg gctcaggaaa     4440

aggccatttt acccttgaat tagtaaagag gtgtaatttc gtaactgcca ttgaaataga     4500

ccataaatta tgcaaaacta cagaaaataa acttgttgat cacgataatt tccaagtttt     4560

aaacaaggat atattgcagt ttaaatttcc taaaaaccaa tcctataaaa tatatggtaa     4620

tataccttat aacataagta cggatataat acgcaaaatt gtttttgata gtatagctaa     4680

tgagatttat ttaatcgtgg aatacgggtt tgctaaaaga ttattaaata caaaacgctc     4740

attggcatta cttttaatgg cagaagttga tatttctata ttaagtatgg ttccaagaga     4800

atattttcat cctaaaccta aagtgaatag ctcacttatc agattaagta gaaaaaaatc     4860

aagaatatca cacaaagata aacaaaagta taattatttc gttatgaaat gggttaacaa     4920

agaatacaag aaaatattta caaaaaatca atttaacaat tccttaaaac atgcaggaat     4980

tgacgattta aacaatatta gctttgaaca attcttatct cttttcaata gctataaatt     5040

atttaataag taagttaagg gatgcataaa ctgcatccct taacttgttt ttcgtgtgcc     5100

tattttttgt gaatcgacct gcaggcatgc aagcttaagc gagttggaat ttaaatatga     5160

tatctacatt atcagcagta acatcaacct ttgatacaag gttgttgacg attttctttt     5220

tattatcata tgatagttca ttaatcggaa ttgagcccaa ctgagtttta actaactcaa     5280

aaacatcagt agagtcatta aatttatttt cgctaatctt agctttaagc agctttttct     5340

cagcctgaag ggaatcagta cgatctttca actcatccat agtgataaaa tcatttaggt     5400

acaaatcaga gttcttttgt atttttttat cgatctgtga aatttgcttt ttaaatgacg     5460

aagtatcaag aataggttgg ttgttgccat tgataatttt caataaggag tcattatttt     5520

cttgaaatcc aatcaggttg tcaataacag tattttctaa attacttaaa tcataagttc     5580

ctgaatcaca ctttttattg tcattatata ctgtaattcc ttttgttttt cgaggaaatc     5640

tatttgcaca gtgatatttc atagtgcggc ttccatcttt tcttttgtgg ccaagaacaa     5700

tttttaaagg tgctccacag taaccgcacc ttgccatccc tgacagcata tatttagctt     5760

ggaaaggtct agggttgtta tttctttcat aagtctgctg ttgtctttct tctagctctt     5820

tttgaacttt taaataagtc tcataaggga taattggttt gtgcatacct tcaaataggc     5880

tgtccttaaa tttgatataa ccacagtaaa ctggattatc aagtgtttgt cttagggtac     5940

gataagacca cggtatatct ttaccgatgt gtccagattc attgagttta tctcttaatt     6000

ttgtaagtga tattcctgat aaataatcag tgaatatttg ttcaactatt gtagcttgta     6060

aaggaacaat ttctaatata cctgtctttc tgttgtggta atacccaaaa gctgtcttag     6120

tccacatcat agacttacca gatttcgctc gccctagttt acccatagtc atgcgttctt     6180

ttatattctc tctttcaaac tcattaattg cagaaagaat agtgagaaac aagctaccca     6240

tagcagaaga agtatcaata ctttcattaa gcgagataaa gtctatttta ttttttgtga     6300

acacatcctt aacaagataa agagtatctc ttacactacg tgaaaggcgg tctagcttat     6360

atacaagaac tgtatcaaaa gctttattct cgatatcgtt gattaatctt tgcattgctg     6420

ggcgttcaag tttggcccct gaaaaaccag catcagtata agtatcagat acttgccacc     6480

ccattgcttc agcatatttt gttaaacggt caatttgctc atcaattgag aacccttcct     6540

ctgcttggtt agtagtggat actcgtgtat agattgctac tttcttagtc atgagatttc     6600

ccccttaaaa ataaattcat tcaaatacag atgcatttta tttcatatag taagtacatc     6660

acctattagt ttgttgttta aacaaactaa cttattttca tcttatatag cctcgtcagt     6720

attttcaata ttttttttag ttttttatga acacattaga tataataaag ggaagattcg     6780

ctatgtacta tgttgatact taatttaaag attaaacaaa tggagtggat gaagtggata     6840

tcgctgatca aacctttgtc aaaaaagtaa atcaaaagtt attattaaaa gaaatcctta     6900

aaaattcacc tatttcaaga gcaaaattat ctgaaatgac tggattaaat aaatcaactg     6960

tctcatcaca ggtaaacacg ttaatgaaag aaagtatggt atttgaaata ggtcaaggac     7020

aatcaagtgg cggaagaaga cctgtcatgc ttgtttttaa taaaaaggca ggatactccg     7080

ttggaataga tgttggtgtg gattatatta atggcatttt aacagacctt gaaggaacaa     7140

tcgttcttga tcaataccgc catttggaat ccaattctcc agaaataacg aaagacattt     7200

tgattgatat gattcatcac tttattacgc aaatgcccca atctccgtac gggcttattg     7260

gtataggtat ttgcgtgcct ggactcattg ataaagatca aaaaattgtt ttcactccga     7320

actccaactg gagagatatt gacttaaaat cttcgataca agagaagtac aatgtgcctg     7380

tttttattga aaatgaggca aatgctggcg catatggaga aaaagtattt ggagctgcaa     7440

aaaatcacga taacattatt tacgtaagta tcagcacagg aatagggatc ggtgttatta     7500

tcaacaatca tttatataga ggagtaagcg gcttctctgg agaaatggga catatgacaa     7560

tagactttaa tggtcctaaa tgcagttgcg gaaaccgagg atgctgggaa ttgtatgctt     7620

cagagaaggc tttattaaaa tctcttcaga ccaaagagaa aaaactgtcc tatcaagata     7680

tcataaacct cgcccatctg aatgatatcg gaaccttaaa tgcattacaa aattttggat     7740

tctatttagg aataggcctt accaatattc taaatacttt caacccacaa gccgtaattt     7800

taagaaatag cataattgaa tcgcatccta tggttttaaa ttcaatgaga agtgaagtat     7860

catcaagggt ttattcccaa ttaggcaata gctatgaatt attgccatct tccttaggac     7920

agaatgcacc ggcattagga atgtcctcca ttgtgattga tcattttctg gacatgatta     7980

caatgtaatt ttttatggaa tggacagctc atctttaaag atgagttttt ttattctagg     8040

agtatttctg aattcgagct cgttattaat ctgttcagca atcgggcgcg attgctgaat     8100

aaaagatacg agagacctct cttgtatctt ttttattttg agtggttttg tccgttacac     8160

tagaaaaccg aaagacaata aaaattttat tcttgctgag tctggctttc ggtaagctag     8220

acaaaacgga caaaataaaa attggcaagg gtttaaaggt ggagattttt tgagtgatct     8280

tctcaaaaaa tactacctgt cccttgctga tttttaaacg agcacgagag caaaaccccc     8340

ctttgctgag gtggcagagg gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa     8400

ggtcttaaag gttttatggt tttggtcggc actgccgaca gcctcgcaga gcacacactt     8460

tatgaatata aagtatagtg tgttatactt tacttggaag tggttgccgg aaagagcgaa     8520

aatgcctcac atttgtgcca cctaaaaagg agcgatttac atatgagtta tgcagtttgt     8580

agaatgcaaa aagtgaaatc agctggacta aaaggcagag ctc                       8623


<210>  17
<211>  696
<212>  DNA
<213>  artificial sequence

<220>
<223>  Codon-optimized gene encoding the mature Dictyoglomus 
       thermophilum xylanase (xylanase-domain only) with an artificial 
       secretion signal added to the 5'-end of the CDS.


<220>
<221>  CDS
<222>  (1)..(696)
<223>  The codon-optimized gene of SEQ ID NO:14 encoding the mature 
       Dictyoglomus thermophilum xylanase (xylanase-domain only) with an
       artificial secretion signal (insted of the ATG translational 
       start codon) added to the 5'-end of the CDS.

<220>
<221>  sig_peptide
<222>  (1)..(87)
<223>  Secretion signal peptide.

<220>
<221>  mat_peptide
<222>  (88)..(696)
<223>  Mature Dictyoglomus thermophilum xylanase (xylanase-domain only).

<400>  17
atg aaa caa caa aaa cgg ctt tac gcc cga ttg gtg ctt atg tgc acg         48
Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Val Leu Met Cys Thr           
                -25                 -20                 -15               

ctg tta ttt gtc agt ttg ccg att aca aaa aca tca gcc cag aca tca         96
Leu Leu Phe Val Ser Leu Pro Ile Thr Lys Thr Ser Ala Gln Thr Ser           
            -10                 -5              -1  1                     

atc aca ctt aca tct aac gca tca ggc aca ttc gac ggc tat tac tac        144
Ile Thr Leu Thr Ser Asn Ala Ser Gly Thr Phe Asp Gly Tyr Tyr Tyr           
    5                   10                  15                            

gag ctt tgg aag gac aca ggc aac acg act atg act gta tac act caa        192
Glu Leu Trp Lys Asp Thr Gly Asn Thr Thr Met Thr Val Tyr Thr Gln           
20                  25                  30                  35            

ggt cgc ttc tca tgc cag tgg tct aac atc aac aac gcg ctt ttc cgc        240
Gly Arg Phe Ser Cys Gln Trp Ser Asn Ile Asn Asn Ala Leu Phe Arg           
                40                  45                  50                

acg ggc aag aag tac aac cag aac tgg caa tct ctt ggc act atc cgc        288
Thr Gly Lys Lys Tyr Asn Gln Asn Trp Gln Ser Leu Gly Thr Ile Arg           
            55                  60                  65                    

atc act tat tct gcg aca tac aac ccg aac ggc aac tct tac ctt tgt        336
Ile Thr Tyr Ser Ala Thr Tyr Asn Pro Asn Gly Asn Ser Tyr Leu Cys           
        70                  75                  80                        

atc tac ggc tgg tct acg aac ccg ctt gtt gag ttc tac atc gta gag        384
Ile Tyr Gly Trp Ser Thr Asn Pro Leu Val Glu Phe Tyr Ile Val Glu           
    85                  90                  95                            

tct tgg ggc aac tgg cgt cct cct ggc gca aca tct ctt ggc cag gtt        432
Ser Trp Gly Asn Trp Arg Pro Pro Gly Ala Thr Ser Leu Gly Gln Val           
100                 105                 110                 115           

aca atc gat ggt ggc aca tat gac atc tac cgc act act cgc gtt aac        480
Thr Ile Asp Gly Gly Thr Tyr Asp Ile Tyr Arg Thr Thr Arg Val Asn           
                120                 125                 130               

cag cct agc atc gtt ggc aca gct act ttc gac caa tac tgg agc gtt        528
Gln Pro Ser Ile Val Gly Thr Ala Thr Phe Asp Gln Tyr Trp Ser Val           
            135                 140                 145                   

cgc act agc aag cgc aca tct ggc aca gtt acg gtt acg gac cac ttt        576
Arg Thr Ser Lys Arg Thr Ser Gly Thr Val Thr Val Thr Asp His Phe           
        150                 155                 160                       

cgc gca tgg gca aat cgt ggc ctt aac ctt ggc aca atc gac caa atc        624
Arg Ala Trp Ala Asn Arg Gly Leu Asn Leu Gly Thr Ile Asp Gln Ile           
    165                 170                 175                           

aca ctt tgt gtt gag ggc tac cag tct tct ggc agc gca aac atc act        672
Thr Leu Cys Val Glu Gly Tyr Gln Ser Ser Gly Ser Ala Asn Ile Thr           
180                 185                 190                 195           

caa aac act ttc tct cag ggc agc                                        696
Gln Asn Thr Phe Ser Gln Gly Ser                                           
                200                                                       


<210>  18
<211>  232
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  18

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Val Leu Met Cys Thr 
                -25                 -20                 -15     


Leu Leu Phe Val Ser Leu Pro Ile Thr Lys Thr Ser Ala Gln Thr Ser 
            -10                 -5              -1  1           


Ile Thr Leu Thr Ser Asn Ala Ser Gly Thr Phe Asp Gly Tyr Tyr Tyr 
    5                   10                  15                  


Glu Leu Trp Lys Asp Thr Gly Asn Thr Thr Met Thr Val Tyr Thr Gln 
20                  25                  30                  35  


Gly Arg Phe Ser Cys Gln Trp Ser Asn Ile Asn Asn Ala Leu Phe Arg 
                40                  45                  50      


Thr Gly Lys Lys Tyr Asn Gln Asn Trp Gln Ser Leu Gly Thr Ile Arg 
            55                  60                  65          


Ile Thr Tyr Ser Ala Thr Tyr Asn Pro Asn Gly Asn Ser Tyr Leu Cys 
        70                  75                  80              


Ile Tyr Gly Trp Ser Thr Asn Pro Leu Val Glu Phe Tyr Ile Val Glu 
    85                  90                  95                  


Ser Trp Gly Asn Trp Arg Pro Pro Gly Ala Thr Ser Leu Gly Gln Val 
100                 105                 110                 115 


Thr Ile Asp Gly Gly Thr Tyr Asp Ile Tyr Arg Thr Thr Arg Val Asn 
                120                 125                 130     


Gln Pro Ser Ile Val Gly Thr Ala Thr Phe Asp Gln Tyr Trp Ser Val 
            135                 140                 145         


Arg Thr Ser Lys Arg Thr Ser Gly Thr Val Thr Val Thr Asp His Phe 
        150                 155                 160             


Arg Ala Trp Ala Asn Arg Gly Leu Asn Leu Gly Thr Ile Asp Gln Ile 
    165                 170                 175                 


Thr Leu Cys Val Glu Gly Tyr Gln Ser Ser Gly Ser Ala Asn Ile Thr 
180                 185                 190                 195 


Gln Asn Thr Phe Ser Gln Gly Ser 
                200             


<210>  19
<211>  8603
<212>  DNA
<213>  artificial sequence

<220>
<223>  Plasmid pHyGe396 (see Figure 2; example 4).

<400>  19
cgcgtagggc ccgcggctag cggccgcgtc gactagaaga gcagagagga cggatttcct       60

gaaggaaatc cgttttttta ttttgcccgt cttataaatt tcgttgtcca actcgcttaa      120

ttgcgagttt ttatttcgtt tatttcaatt aaggtaacta aagatcctct agagtcgatt      180

atgtcttttg cgcagtcggc ttaaaccagt tttcgctggt gcgaaaaaag agtgtcttgt      240

gacactctta aattcaaaat ctatcggtca gatttatacc gatttgattt tatatattct      300

tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa tttaaacttc      360

attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat aaactcaatc      420

atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc atcaacatct      480

acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg atcgtccatg      540

tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat agtcgcattc      600

aattcttctg taattatttt tatctgttca taagatttat taccctcata catcactaga      660

atatgataat gctctttttt catcctacct tctgtatcag tatccctatc atgtaatgga      720

gacactacaa attgaatgtg taactctttt aaatactcta accactcggc ttttgctgat      780

tctggatata aaacaaatgt ccaattacgt cctcttgaat ttttcttgtt ttcagtttct      840

tttattacat tttcgctcat gatataataa cggtgctaat acacttaaca aaatttagtc      900

atagataggc agcatgccag tgctgtctat ctttttttgt ttaaaatgca ccgtattcct      960

cctttgcata tttttttatt agaataccgg ttgcatctga tttgctaata ttatattttt     1020

ctttgattct atttaatatc tcattttctt ctgttataag tcttaaagta acagcaactt     1080

ttttctcttc ttttctatct acaaccatca ctgtacctcc caacatctgt ttttttcact     1140

ttaacataaa aaacaacctt ttaacattaa aaacccaata tttatttatt tgtttggaca     1200

atggacaatg gacacctagg ggggaggtcg tagtaccccc ctatgttttc tcccctaaat     1260

aaccccaaaa atctaagaaa aaaagacctc aaaaaggtct ttaattaaca tctcaaattt     1320

cgcatttatt ccaatttcct ttttgcgtgt gatgcgctgc gtccattaaa aatcctagag     1380

ctttgcaacc gaaagttaat agctgtcgct actactttcg cttacgctct aagtatattt     1440

taaggactgt cacacgcaaa aagttttctc ggcataaaag tacctctaca tctctaaatc     1500

gtctgtacgc tgtttctcac gctttctatc gaccttctgg acattatcct gtacaacatc     1560

cataaactgt cccacacgct cgaatttgga atcattaaag aatttctctt taagcctatt     1620

aaaccctttc tcaaacccag ggaaattcgc cctcgcagca cgatataaag tcactgtact     1680

atcttgaaat ttctctgata cattcaactg ctcattcaaa ctatcattct ctcgctttaa     1740

tttattaacc tctttacttt tttcgtgata cccctctttc catgtattca ctacttcttt     1800

caaactctct ctacgttttt ttaattcttg attttctgtg taatagtctg tgctcttaat     1860

attttcgtaa tcatcaacaa tccgttctgc agaagagatt gtttcttgca ggcgttcaaa     1920

ttcatcagca gttaatatct ttctaccagt ctcttcacgt ccagagaaca aacctgtacg     1980

ctcattttca taatcaaagg gtttcgtaga cctcatatgc tctattccac tctgtaactg     2040

cttatttgcc ttctgtaact catccttaac ttcttgcagt tcctgtttat gaaatacagt     2100

atctttcttg tactgatcca tcgctttatg ttctcgttct gtaacctctt tggacgtgcc     2160

tctttcaagt tcataacctt tctcattcac atactcatta aatctatctt gtaattgagt     2220

aaagtctttc ttgttgccta actgttcttt tgcagacaat ctcccgtcct ctgttaaagg     2280

gacaaaacca aagtgcatat gtgggactct ttcatccaga tggacagtcg catacagcat     2340

attttcctta ccgtattcat tttctagaaa ctccaagcta tctttaaaaa atcgttctat     2400

ttcttctccg cttaaatcat caaagaaatc tttatcactt gtaaccagtc cgtccacatg     2460

tcgaattgca tctgaccgaa ttttacgttt ccctgaataa ttctcatcaa tcgtttcatc     2520

aattttatct ttatacttta tattttgtgc gttaatcaaa tcataatttt tatatgtttc     2580

ctcatgattt atgtctttat tattatagtt tttattctct ctttgattat gtctttgtat     2640

cccgtttgta ttacttgatc ctttaactct ggcaaccctc aaaattgaat gagacatgct     2700

acacctccgg ataataaata tatataaacg tatatagatt tcataaagtc taacacacta     2760

gacttattta cttcgtaatt aagtcgttaa accgtgtgct ctacgaccaa aactataaaa     2820

cctttaagaa ctttcttttt ttacaagaaa aaagaaatta gataaatctc tcatatcttt     2880

tattcaataa tcgcatccga ttgcagtata aatttaacga tcactcatca tgttcatatt     2940

tatcagtgct cgtgctataa ttatactaat tttataagga ggaaaaaata tgggcatttt     3000

tagtattttt gtaatcagca cagttcatta tcaaccaaac aaaaaataag tggttataat     3060

gaatcgttaa taagcaaaat tcatataacc aaattaaaga gggttataat gaacgagaaa     3120

aatataaaac acagtcaaaa ctttattact tcaaaacata atatagataa aataatgaca     3180

aatataagat taaatgaaca tgataatatc tttgaaatcg gctcaggaaa aggccatttt     3240

acccttgaat tagtaaagag gtgtaatttc gtaactgcca ttgaaataga ccataaatta     3300

tgcaaaacta cagaaaataa acttgttgat cacgataatt tccaagtttt aaacaaggat     3360

atattgcagt ttaaatttcc taaaaaccaa tcctataaaa tatatggtaa tataccttat     3420

aacataagta cggatataat acgcaaaatt gtttttgata gtatagctaa tgagatttat     3480

ttaatcgtgg aatacgggtt tgctaaaaga ttattaaata caaaacgctc attggcatta     3540

cttttaatgg cagaagttga tatttctata ttaagtatgg ttccaagaga atattttcat     3600

cctaaaccta aagtgaatag ctcacttatc agattaagta gaaaaaaatc aagaatatca     3660

cacaaagata aacaaaagta taattatttc gttatgaaat gggttaacaa agaatacaag     3720

aaaatattta caaaaaatca atttaacaat tccttaaaac atgcaggaat tgacgattta     3780

aacaatatta gctttgaaca attcttatct cttttcaata gctataaatt atttaataag     3840

taagttaagg gatgcataaa ctgcatccct taacttgttt ttcgtgtgcc tattttttgt     3900

gaatcgacct gcaggcatgc aagcttaagc gagttggaat ttaaatatga tatctacatt     3960

atcagcagta acatcaacct ttgatacaag gttgttgacg attttctttt tattatcata     4020

tgatagttca ttaatcggaa ttgagcccaa ctgagtttta actaactcaa aaacatcagt     4080

agagtcatta aatttatttt cgctaatctt agctttaagc agctttttct cagcctgaag     4140

ggaatcagta cgatctttca actcatccat agtgataaaa tcatttaggt acaaatcaga     4200

gttcttttgt atttttttat cgatctgtga aatttgcttt ttaaatgacg aagtatcaag     4260

aataggttgg ttgttgccat tgataatttt caataaggag tcattatttt cttgaaatcc     4320

aatcaggttg tcaataacag tattttctaa attacttaaa tcataagttc ctgaatcaca     4380

ctttttattg tcattatata ctgtaattcc ttttgttttt cgaggaaatc tatttgcaca     4440

gtgatatttc atagtgcggc ttccatcttt tcttttgtgg ccaagaacaa tttttaaagg     4500

tgctccacag taaccgcacc ttgccatccc tgacagcata tatttagctt ggaaaggtct     4560

agggttgtta tttctttcat aagtctgctg ttgtctttct tctagctctt tttgaacttt     4620

taaataagtc tcataaggga taattggttt gtgcatacct tcaaataggc tgtccttaaa     4680

tttgatataa ccacagtaaa ctggattatc aagtgtttgt cttagggtac gataagacca     4740

cggtatatct ttaccgatgt gtccagattc attgagttta tctcttaatt ttgtaagtga     4800

tattcctgat aaataatcag tgaatatttg ttcaactatt gtagcttgta aaggaacaat     4860

ttctaatata cctgtctttc tgttgtggta atacccaaaa gctgtcttag tccacatcat     4920

agacttacca gatttcgctc gccctagttt acccatagtc atgcgttctt ttatattctc     4980

tctttcaaac tcattaattg cagaaagaat agtgagaaac aagctaccca tagcagaaga     5040

agtatcaata ctttcattaa gcgagataaa gtctatttta ttttttgtga acacatcctt     5100

aacaagataa agagtatctc ttacactacg tgaaaggcgg tctagcttat atacaagaac     5160

tgtatcaaaa gctttattct cgatatcgtt gattaatctt tgcattgctg ggcgttcaag     5220

tttggcccct gaaaaaccag catcagtata agtatcagat acttgccacc ccattgcttc     5280

agcatatttt gttaaacggt caatttgctc atcaattgag aacccttcct ctgcttggtt     5340

agtagtggat actcgtgtat agattgctac tttcttagtc atgagatttc ccccttaaaa     5400

ataaattcat tcaaatacag atgcatttta tttcatatag taagtacatc acctattagt     5460

ttgttgttta aacaaactaa cttattttca tcttatatag cctcgtcagt attttcaata     5520

ttttttttag ttttttatga acacattaga tataataaag ggaagattcg ctatgtacta     5580

tgttgatact taatttaaag attaaacaaa tggagtggat gaagtggata tcgctgatca     5640

aacctttgtc aaaaaagtaa atcaaaagtt attattaaaa gaaatcctta aaaattcacc     5700

tatttcaaga gcaaaattat ctgaaatgac tggattaaat aaatcaactg tctcatcaca     5760

ggtaaacacg ttaatgaaag aaagtatggt atttgaaata ggtcaaggac aatcaagtgg     5820

cggaagaaga cctgtcatgc ttgtttttaa taaaaaggca ggatactccg ttggaataga     5880

tgttggtgtg gattatatta atggcatttt aacagacctt gaaggaacaa tcgttcttga     5940

tcaataccgc catttggaat ccaattctcc agaaataacg aaagacattt tgattgatat     6000

gattcatcac tttattacgc aaatgcccca atctccgtac gggcttattg gtataggtat     6060

ttgcgtgcct ggactcattg ataaagatca aaaaattgtt ttcactccga actccaactg     6120

gagagatatt gacttaaaat cttcgataca agagaagtac aatgtgcctg tttttattga     6180

aaatgaggca aatgctggcg catatggaga aaaagtattt ggagctgcaa aaaatcacga     6240

taacattatt tacgtaagta tcagcacagg aatagggatc ggtgttatta tcaacaatca     6300

tttatataga ggagtaagcg gcttctctgg agaaatggga catatgacaa tagactttaa     6360

tggtcctaaa tgcagttgcg gaaaccgagg atgctgggaa ttgtatgctt cagagaaggc     6420

tttattaaaa tctcttcaga ccaaagagaa aaaactgtcc tatcaagata tcataaacct     6480

cgcccatctg aatgatatcg gaaccttaaa tgcattacaa aattttggat tctatttagg     6540

aataggcctt accaatattc taaatacttt caacccacaa gccgtaattt taagaaatag     6600

cataattgaa tcgcatccta tggttttaaa ttcaatgaga agtgaagtat catcaagggt     6660

ttattcccaa ttaggcaata gctatgaatt attgccatct tccttaggac agaatgcacc     6720

ggcattagga atgtcctcca ttgtgattga tcattttctg gacatgatta caatgtaatt     6780

ttttatggaa tggacagctc atctttaaag atgagttttt ttattctagg agtatttctg     6840

aattcgtgct cattattaat ctgttcagca atcgggcgcg attgctgaat aaaagatacg     6900

agagacctct cttgtatctt ttttattttg agtggttttg tccgttacac tagaaaaccg     6960

aaagacaata aaaattttat tcttgctgag tctggctttc ggtaagctag acaaaacgga     7020

caaaataaaa attggcaagg gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa     7080

tactacctgt cccttgctga tttttaaacg agcacgagag caaaaccccc ctttgctgag     7140

gtggcagagg gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag     7200

gttttatggt tttggtcggc actgccgaca gcctcgcaga gcacacactt tatgaatata     7260

aagtatagtg tgttatactt tacttggaag tggttgccgg aaagagcgaa aatgcctcac     7320

attaaagata atatctttga attgtaacgc ccctcaaaag taagaactac aaaaaaagaa     7380

tacgttatat agaaatatgt ttgaaccttc ttcagattac aaatatattc ggacggactc     7440

tacctcaaat gcttatctaa ctatagaatg acatacaagc acaaccttga aaatttgaaa     7500

atataactac caatgaactt gttcatgtga attatcgctg tatttaattt tctcaattca     7560

atatataata tgccaataca ttgttacaag tagaaattaa gacacccttg atagccttac     7620

tatacctaac atgatgtagt attaaatgaa tatgtaaata tatttatgat aagaagcgac     7680

ttatttataa tcattacata tttttctatt ggaatgatta agattccaat agaatagtgt     7740

ataaattatt tatcttgaaa ggagggatgc ctaaaaacga agaacattaa aaacatatat     7800

ttgcaccgtc taatggattt atgaaaaatc attttatcag tttgaaaatt atgtattatg     7860

gagctcggta ccctatgttt cacattgaaa ggggaggaga atcatgaaac aacaaaaacg     7920

gctttacgcc cgattggtgc ttatgtgcac gctgttattt gtcagtttgc cgattacaaa     7980

aacatcagcc cagacatcaa tcacacttac atctaacgca tcaggcacat tcgacggcta     8040

ttactacgag ctttggaagg acacaggcaa cacgactatg actgtataca ctcaaggtcg     8100

cttctcatgc cagtggtcta acatcaacaa cgcgcttttc cgcacgggca agaagtacaa     8160

ccagaactgg caatctcttg gcactatccg catcacttat tctgcgacat acaacccgaa     8220

cggcaactct tacctttgta tctacggctg gtctacgaac ccgcttgttg agttctacat     8280

cgtagagtct tggggcaact ggcgtcctcc tggcgcaaca tctcttggcc aggttacaat     8340

cgatggtggc acatatgaca tctaccgcac tactcgcgtt aaccagccta gcatcgttgg     8400

cacagctact ttcgaccaat actggagcgt tcgcactagc aagcgcacat ctggcacagt     8460

tacggttacg gaccactttc gcgcatgggc aaatcgtggc cttaaccttg gcacaatcga     8520

ccaaatcaca ctttgtgttg agggctacca gtcttctggc agcgcaaaca tcactcaaaa     8580

cactttctct cagggcagct aaa                                             8603


