                               SEQUENCE LISTING

<110> PRESIDENT AND FELLOWS OF HARVARD COLLEGE
 
<120> METHODS AND COMPOSITIONS RELATING TO MODULATION OF THE 
      PERMEABILITY OF THE BLOOD BRAIN BARRIER

<130> 002806-075954-PCT

<140>
<141>

<150> 61/912,637
<151> 2013-12-06

<150> 61/876,406
<151> 2013-09-11

<150> 61/839,059
<151> 2013-06-25

<150> 61/837,782
<151> 2013-06-21

<160> 7     

<170> PatentIn version 3.5

<210> 1
<211> 543
<212> PRT
<213> Homo sapiens

<400> 1
Met Ala Lys Gly Glu Gly Ala Glu Ser Gly Ser Ala Ala Gly Leu Leu 
1               5                   10                  15      


Pro Thr Ser Ile Leu Gln Ser Thr Glu Arg Pro Ala Gln Val Lys Lys 
            20                  25                  30          


Glu Pro Lys Lys Lys Lys Gln Gln Leu Ser Val Cys Asn Lys Leu Cys 
        35                  40                  45              


Tyr Ala Leu Gly Gly Ala Pro Tyr Gln Val Thr Gly Cys Ala Leu Gly 
    50                  55                  60                  


Phe Phe Leu Gln Ile Tyr Leu Leu Asp Val Ala Gln Lys Asp Glu Glu 
65                  70                  75                  80  


Val Val Phe Cys Phe Ser Ser Phe Gln Val Gly Pro Phe Ser Ala Ser 
                85                  90                  95      


Ile Ile Leu Phe Val Gly Arg Ala Trp Asp Ala Ile Thr Asp Pro Leu 
            100                 105                 110         


Val Gly Leu Cys Ile Ser Lys Ser Pro Trp Thr Cys Leu Gly Arg Leu 
        115                 120                 125             


Met Pro Trp Ile Ile Phe Ser Thr Pro Leu Ala Val Ile Ala Tyr Phe 
    130                 135                 140                 


Leu Ile Trp Phe Val Pro Asp Phe Pro His Gly Gln Thr Tyr Trp Tyr 
145                 150                 155                 160 


Leu Leu Phe Tyr Cys Leu Phe Glu Thr Met Val Thr Cys Phe His Val 
                165                 170                 175     


Pro Tyr Ser Ala Leu Thr Met Phe Ile Ser Thr Glu Gln Thr Glu Arg 
            180                 185                 190         


Asp Ser Ala Thr Ala Tyr Arg Met Thr Val Glu Val Leu Gly Thr Val 
        195                 200                 205             


Leu Gly Thr Ala Ile Gln Gly Gln Ile Val Gly Gln Ala Asp Thr Pro 
    210                 215                 220                 


Cys Phe Gln Asp Leu Asn Ser Ser Thr Val Ala Ser Gln Ser Ala Asn 
225                 230                 235                 240 


His Thr His Gly Thr Thr Ser His Arg Glu Thr Gln Lys Ala Tyr Leu 
                245                 250                 255     


Leu Ala Ala Gly Val Ile Val Cys Ile Tyr Ile Ile Cys Ala Val Ile 
            260                 265                 270         


Leu Ile Leu Gly Val Arg Glu Gln Arg Glu Pro Tyr Glu Ala Gln Gln 
        275                 280                 285             


Ser Glu Pro Ile Ala Tyr Phe Arg Gly Leu Arg Leu Val Met Ser His 
    290                 295                 300                 


Gly Pro Tyr Ile Lys Leu Ile Thr Gly Phe Leu Phe Thr Ser Leu Ala 
305                 310                 315                 320 


Phe Met Leu Val Glu Gly Asn Phe Val Leu Phe Cys Thr Tyr Thr Leu 
                325                 330                 335     


Gly Phe Arg Asn Glu Phe Gln Asn Leu Leu Leu Ala Ile Met Leu Ser 
            340                 345                 350         


Ala Thr Leu Thr Ile Pro Ile Trp Gln Trp Phe Leu Thr Arg Phe Gly 
        355                 360                 365             


Lys Lys Thr Ala Val Tyr Val Gly Ile Ser Ser Ala Val Pro Phe Leu 
    370                 375                 380                 


Ile Leu Val Ala Leu Met Glu Ser Asn Leu Ile Ile Thr Tyr Ala Val 
385                 390                 395                 400 


Ala Val Ala Ala Gly Ile Ser Val Ala Ala Ala Phe Leu Leu Pro Trp 
                405                 410                 415     


Ser Met Leu Pro Asp Val Ile Asp Asp Phe His Leu Lys Gln Pro His 
            420                 425                 430         


Phe His Gly Thr Glu Pro Ile Phe Phe Ser Phe Tyr Val Phe Phe Thr 
        435                 440                 445             


Lys Phe Ala Ser Gly Val Ser Leu Gly Ile Ser Thr Leu Ser Leu Asp 
    450                 455                 460                 


Phe Ala Gly Tyr Gln Thr Arg Gly Cys Ser Gln Pro Glu Arg Val Lys 
465                 470                 475                 480 


Phe Thr Leu Asn Met Leu Val Thr Met Ala Pro Ile Val Leu Ile Leu 
                485                 490                 495     


Leu Gly Leu Leu Leu Phe Lys Met Tyr Pro Ile Asp Glu Glu Arg Arg 
            500                 505                 510         


Arg Gln Asn Lys Lys Ala Leu Gln Ala Leu Arg Asp Glu Ala Ser Ser 
        515                 520                 525             


Ser Gly Cys Ser Glu Thr Asp Ser Thr Glu Leu Ala Ser Ile Leu 
    530                 535                 540             


<210> 2
<211> 2236
<212> DNA
<213> Homo sapiens

<400> 2
agaactataa gaggcgcgga gggggcgtgc agcagagtgc gttcctcgtc tgccagccgg       60

cttggctagc gcgcggcggc cgtggctaag gctgctacga agcgagcttg ggaggagcag      120

cggcctgcgg ggcagaggag catcccgtct accaggtccc aagcggcgtg gcccgcgggt      180

catggccaaa ggagaaggcg ccgagagcgg ctccgcggcg gggctgctac ccaccagcat      240

cctccaaagc actgaacgcc cggcccaggt gaagaaagaa ccgaaaaaga agaaacaaca      300

gttgtctgtt tgcaacaagc tttgctatgc acttggggga gccccctacc aggtgacggg      360

ctgtgccctg ggtttcttcc ttcagatcta cctattggat gtggctcaga aggatgagga      420

agttgtcttt tgcttctcct cattccaggt gggccctttc tctgcctcca tcatcctgtt      480

tgtgggccga gcctgggatg ccatcacaga ccccctggtg ggcctctgca tcagcaaatc      540

cccctggacc tgcctgggtc gccttatgcc ctggatcatc ttctccacgc ccctggccgt      600

cattgcctac ttcctcatct ggttcgtgcc cgacttccca cacggccaga cctattggta      660

cctgcttttc tattgcctct ttgaaacaat ggtcacgtgt ttccatgttc cctactcggc      720

tctcaccatg ttcatcagca ccgagcagac tgagcgggat tctgccaccg cctatcggat      780

gactgtggaa gtgctgggca cagtgctggg cacggcgatc cagggacaaa tcgtgggcca      840

agcagacacg ccttgtttcc aggacctcaa tagctctaca gtagcttcac aaagtgccaa      900

ccatacacat ggcaccacct cacacaggga aacgcaaaag gcatacctgc tggcagcggg      960

ggtcattgtc tgtatctata taatctgtgc tgtcatcctg atcctgggcg tgcgggagca     1020

gagagaaccc tatgaagccc agcagtctga gccaatcgcc tacttccggg gcctacggct     1080

ggtcatgagc cacggcccat acatcaaact tattactggc ttcctcttca cctccttggc     1140

tttcatgctg gtggagggga actttgtctt gttttgcacc tacaccttgg gcttccgcaa     1200

tgaattccag aatctactcc tggccatcat gctctcggcc actttaacca ttcccatctg     1260

gcagtggttc ttgacccggt ttggcaagaa gacagctgta tatgttggga tctcatcagc     1320

agtgccattt ctcatcttgg tggccctcat ggagagtaac ctcatcatta catatgcggt     1380

agctgtggca gctggcatca gtgtggcagc tgccttctta ctaccctggt ccatgctgcc     1440

tgatgtcatt gacgacttcc atctgaagca gccccacttc catggaaccg agcccatctt     1500

cttctccttc tatgtcttct tcaccaagtt tgcctctgga gtgtcactgg gcatttctac     1560

cctcagtctg gactttgcag ggtaccagac ccgtggctgc tcgcagccgg aacgtgtcaa     1620

gtttacactg aacatgctcg tgaccatggc tcccatagtt ctcatcctgc tgggcctgct     1680

gctcttcaaa atgtacccca ttgatgagga gaggcggcgg cagaataaga aggccctgca     1740

ggcactgagg gacgaggcca gcagctctgg ctgctcagaa acagactcca cagagctggc     1800

tagcatcctc tagggcccgc cacgttgccc gaagccacca tgcagaaggc cacagaaggg     1860

atcaggacct gtctgccggc ttgctgagca gctggactgc aggtgctagg aagggaactg     1920

aagactcaag gaggtggccc aggacacttg ctgtgctcac tgtggggccg gctgctctgt     1980

ggcctcctgc ctcccctctg cctgcctgtg gggccaagcc ctggggctgc cactgtgaat     2040

atgccaagga ctgatcgggc ctagcccgga acactaatgt agaaaccttt ttttttacag     2100

agcctaatta ataacttaat gactgtgtac atagcaatgt gtgtgtatgt atatgtctgt     2160

gagctattaa tgttattaat tttcataaaa gctggaaagc aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaa                                                     2236


<210> 3
<211> 533
<212> PRT
<213> Mus sp.

<400> 3
Met Ala Lys Gly Glu Gly Ala Glu Ser Gly Ser Ala Ala Gly Leu Leu 
1               5                   10                  15      


Pro Thr Ser Ile Leu Gln Ala Ser Glu Arg Pro Val Gln Val Lys Lys 
            20                  25                  30          


Glu Pro Lys Lys Lys Gln Gln Leu Ser Ile Cys Asn Lys Leu Cys Tyr 
        35                  40                  45              


Ala Val Gly Gly Ala Pro Tyr Gln Leu Thr Gly Cys Ala Leu Gly Phe 
    50                  55                  60                  


Phe Leu Gln Ile Tyr Leu Leu Asp Val Ala Lys Val Glu Pro Leu Pro 
65                  70                  75                  80  


Ala Ser Ile Ile Leu Phe Val Gly Arg Ala Trp Asp Ala Phe Thr Asp 
                85                  90                  95      


Pro Leu Val Gly Phe Cys Ile Ser Lys Ser Ser Trp Thr Arg Leu Gly 
            100                 105                 110         


Arg Leu Met Pro Trp Ile Ile Phe Ser Thr Pro Leu Ala Ile Ile Ala 
        115                 120                 125             


Tyr Phe Leu Ile Trp Phe Val Pro Asp Phe Pro Ser Gly Thr Glu Ser 
    130                 135                 140                 


Ser His Gly Phe Leu Trp Tyr Leu Leu Phe Tyr Cys Leu Phe Glu Thr 
145                 150                 155                 160 


Leu Val Thr Cys Phe His Val Pro Tyr Ser Ala Leu Thr Met Phe Ile 
                165                 170                 175     


Ser Thr Glu Gln Ser Glu Arg Asp Ser Ala Thr Ala Tyr Arg Met Thr 
            180                 185                 190         


Val Glu Val Leu Gly Thr Val Ile Gly Thr Ala Ile Gln Gly Gln Ile 
        195                 200                 205             


Val Gly Gln Ala Lys Ala Pro Cys Leu Gln Asp Gln Asn Gly Ser Val 
    210                 215                 220                 


Val Val Ser Glu Val Ala Asn Arg Thr Gln Ser Thr Ala Ser Leu Lys 
225                 230                 235                 240 


Asp Thr Gln Asn Ala Tyr Leu Leu Ala Ala Gly Ile Ile Ala Ser Ile 
                245                 250                 255     


Tyr Val Leu Cys Ala Phe Ile Leu Ile Leu Gly Val Arg Glu Gln Arg 
            260                 265                 270         


Glu Leu Tyr Glu Ser Gln Gln Ala Glu Ser Met Pro Phe Phe Gln Gly 
        275                 280                 285             


Leu Arg Leu Val Met Gly His Gly Pro Tyr Val Lys Leu Ile Ala Gly 
    290                 295                 300                 


Phe Leu Phe Thr Ser Ala Phe Met Leu Val Glu Gly Asn Phe Ala Leu 
305                 310                 315                 320 


Phe Cys Thr Tyr Thr Leu Asp Phe Arg Asn Glu Phe Gln Asn Leu Leu 
                325                 330                 335     


Leu Ala Ile Met Leu Ser Ala Thr Phe Thr Ile Pro Ile Trp Gln Trp 
            340                 345                 350         


Phe Leu Thr Arg Phe Gly Lys Lys Thr Ala Val Tyr Ile Gly Ile Ser 
        355                 360                 365             


Ser Ala Val Pro Phe Leu Ile Leu Val Ala Leu Met Glu Arg Asn Leu 
    370                 375                 380                 


Ile Val Thr Tyr Val Val Ala Val Ala Ala Gly Val Ser Val Ala Ala 
385                 390                 395                 400 


Ala Phe Leu Leu Pro Trp Ser Met Leu Pro Asp Val Ile Asp Asp Phe 
                405                 410                 415     


His Leu Lys His Pro His Ser Pro Gly Thr Glu Pro Ile Phe Phe Ser 
            420                 425                 430         


Phe Tyr Val Phe Phe Thr Lys Phe Ala Ser Gly Val Ser Leu Gly Val 
        435                 440                 445             


Ser Thr Leu Ser Leu Asp Phe Ala Asn Tyr Gln Arg Gln Gly Cys Ser 
    450                 455                 460                 


Gln Pro Glu Gln Val Lys Phe Thr Leu Lys Met Leu Val Thr Met Ala 
465                 470                 475                 480 


Pro Ile Ile Leu Ile Leu Leu Gly Leu Leu Leu Phe Lys Leu Tyr Pro 
                485                 490                 495     


Ile Asp Glu Glu Lys Arg Arg Gln Asn Lys Lys Ala Leu Gln Ala Leu 
            500                 505                 510         


Arg Glu Glu Ala Ser Ser Ser Gly Cys Ser Asp Thr Asp Ser Thr Glu 
        515                 520                 525             


Leu Ala Ser Ile Leu 
    530             


<210> 4
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
cctggtttgc taagtgctag c                                                 21


<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 5
gttcactggc ttggaggatg c                                                 21


<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
cacttcctaa agccttactt c                                                 21


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
gcagcgcatc gccttctatc                                                   20


