                         SEQUENCE LISTING

<110>  Demaria, Marco
       Campisi, Judith
 
<120>  METHODS FOR ENHANCING EFFECTIVENESS OF MEDICAL THERAPIES

<130>  200201.416WO

<140>  PCT
<141>  2014-06-19

<150>  US 61/837,089
<151>  2013-06-19

<160>  39 

<170>  PatentIn version 3.5

<210>  1
<211>  9267
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBLUESCRIPT II KS vector containing a p16Ink4a 
       promoter-FKBP-caspase-IRES-GFP nucleic acid construct

<400>  1
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca      660

ccgcggtggc ggccgctcta gaactagtgg atccgtgtaa agtcactgct tttatagcta      720

catctgcata gatcccctgt atgaaagcat gtactacctg gataataata tctgtatttt      780

tctgtagtag gaaatcagtg tagtttttaa aaccaaaaag tattgttatt aatctatctt      840

tgatctcaaa caatttcaat gacctagtat agtgatttct acggaaagcc ctgcaattta      900

ctcaaagcag tttttaaata ttgttttaaa agtgtgtgtg tgtgtgtgtg tgtgtgtgtg      960

tgtgtgtgtg gtgttaaagt cattttcaaa cccctcacaa tgtcttgaat gtgacatttg     1020

agtcatttat ggtaacttat aactcctttg aagaagttat tcagaattga ggttccagac     1080

acacaaatgc acaatacacc atttttcctt ccagttaaca atcagagggc aacacttatt     1140

tttaaaggaa aatcgactcc ataagggact ttataaaggg gtagacataa accagtatca     1200

gggataaact ctccgttccc ctgtttaacc taattttccc agggccatcc tggaatacga     1260

attttctctt gaaatacagt caaagaaaaa gtggtaggct acagagcaga ggaaacactg     1320

gacacagcga cccaccccag agtcacttcc cttaatctaa tgactaggtt ttttctgaaa     1380

gttattttgt tagaacacag gaacttttgc gaccacagtg atgcttttag agggttgaat     1440

cctcaaaaag aaaattaatc gcaactagta gaagggagat tacttattga ttcttataac     1500

ttctgcagga atacacagtt atgagttagg gcaaagagaa aattgacttt taatattctc     1560

tatcactaac atgagagaac atgtatgtgt tccaaaataa tttttattta ttgaaaaccc     1620

gctatatacc tggattttca cagaatattc attactctcc aaaatggcct tttctaggtg     1680

aattttattt tccttacaga cctcaagaag tttacataat ttacttaaac ctgaggagag     1740

agaacaaagc ctcagaaaat ttacatagtt tatttaaact aaactcagct tgcttggtag     1800

cagcttctaa tcccagcagt taaagagaca gaagcagggc caacctgggg tataatataa     1860

ggtgagactc tcctttcttt ctctctgtct ctgtctgtct ctgtctctgt gtgtgtgtgt     1920

gtgtgtgtgt gtgtgtgtgt gtgtctcctc tctctctctc tctctctctc tctctctctc     1980

tctgtctctc tctccctccc cctccctccc tctccccctc ctctctccct ccctctccct     2040

cccccccccc cacacatttg aattcgtgga gttggtaaat gaggggtcag ttctctgtct     2100

gtctgtagtt ttgtgtccac aggatatgac tgacattctc accacacaca tacaaagtca     2160

aaaatagctg tggccatata aagaatatgg ggagagaaaa ttattcaaaa tctgcagaaa     2220

ataatgccag gcctttaatc ctggcaccca ggaggcagaa gggagacaga gttctgagtt     2280

tatgctgagt tccaggagtg gaagaaaggg ccattgcctt tctggtgagg actgtctttt     2340

taaatcctcc cttctgtcca gtactggtaa ctctgcccaa agcgtgttct tcttcctgcc     2400

tcacaagatt gcaaagacgt ttttaacgaa caatttaaac cggtgcaacg tttatgcgca     2460

gcacaccaac tcatttaaac aaacaacagc cccataaaat agaaatactt tataagcaga     2520

ttgccctccg atgacttcac cccgtcactt ttttatagtt gtgtacagaa tcctagcact     2580

gatacagcaa catcagaaat gtttctgcaa atccttcgca aagattcgga tttcatactg     2640

ggcgtggtac cctccaaaat gagttgtttg agctagggtt gttgggatct cagcttggcg     2700

aagttgtagc tctttcttct gaataaaaga tgacacaatt ttctgctaag atgttaaata     2760

ccttaagttt cagtgtagtg atgaaaatta ccctccttcg tttttctaat acctgggtgt     2820

tgcactgggg aggaaggaga gatttcgaga aggactagtt cactttctca gaagacacgt     2880

gtgcacttct ttgctgtgcg ggtccagaag gagcccagcg tgtcaaaggg tgaccaggca     2940

tgggggaggg gtgttagcgt gggtagcagg cgggggctgt ccgatccttt agcgctgttt     3000

caacgcccag ctctcctcct gaaccctgca tctcttctgt agtccgggct ccatcccttt     3060

cccctccccc atccggaggt ggggggaaca gcagtgtttt caggggtgtt caattcatgc     3120

tatattcagg gcaaatagcg ccacctatgg cgggctgtgg agccaggtca ggagcagagt     3180

gtggctcccc ccccccccca caccatcctc agaggaagga aggagggacc cactggtcac     3240

acgactgggc gattgggcgg gcactgaatc tccgcgagga aagcgaactc gaggagagcc     3300

atcacgcgta gcatggggag tagcaagagc aagcctaagg accccagcca gcgctctaga     3360

ggcgtccaag tcgaaaccat tagtcccggc gatggcagaa catttcctaa aaggggacaa     3420

acatgtgtcg tccattatac aggcatgttg gaggacggca aaaaggtgga cagtagtaga     3480

gatcgcaata aacctttcaa attcatgttg ggaaaacaag aagtcattag gggatgggag     3540

gagggcgtgg ctcaaatgtc cgtcggccaa cgcgctaagc tcaccatcag ccccgactac     3600

gcatacggcg ctaccggaca tcccggaatt attccccctc acgctacctt ggtgtttgac     3660

gtcgaactgt tgaagctcga gactagagga gtgcaggtgg agactatctc cccaggagac     3720

gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa     3780

gatggaaaga aagttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc     3840

aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga     3900

gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc     3960

ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtagtgaa     4020

tcacagactt tggacaaagt ttaccaaatg aaaagcaaac ctcggggata ctgtctgatc     4080

atcaacaatc acaattttgc aaaagcacgg gagaaagtgc ccaaacttca cagcattagg     4140

gacaggaatg gaacacactt ggatgcaggg gctttgacca cgacctttga agagcttcat     4200

tttgagatca agccccacga tgactgcaca gtagagcaaa tctatgagat tttgaaaatc     4260

taccaactca tggaccacag taacatggac tgcttcatct gctgtatcct ctcccatgga     4320

gacaagggca tcatctatgg cactgatgga caggaggccc ccatctatga gctgacatct     4380

cagttcactg gtttgaagtg cccttccctt gctggaaaac ccaaagtgtt ttttattcag     4440

gcttgtcagg gggataacta ccagaaaggt atacctgttg agactgattc agaggagcaa     4500

ccctatttag aaatggattt atcatcacct caaacgagat atatcccgga tgaggctgac     4560

tttctgctgg ggatggccac tgtgaataac tgtgtttcct accgaaaccc tgcagaggga     4620

acctggtaca tccagtcact ttgccagagc ctgagagagc gatgtcctcg aggcgatgat     4680

attctcacca tcctgactga agtgaactat gaagtaagca acaaggatga caagaaaaac     4740

atggggaaac agatgcctca gcctactttc acactaagaa aaaaacttgt cttcccttct     4800

gatgattaca aggatgacga cgataagtga ggatcaacct cgaggaattc acgcgtttaa     4860

ttaactcgag gttttcgagg tcgacggtat cgataagctt gatatcgaat tccgcccctc     4920

tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt     4980

tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc     5040

tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca     5100

aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac     5160

gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg     5220

ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt     5280

gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct     5340

gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg     5400

ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt     5460

ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg     5520

agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca     5580

agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt     5640

tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct     5700

acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt     5760

ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact     5820

acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga     5880

agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca     5940

acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca     6000

agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca     6060

cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg     6120

ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg     6180

ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gatctttttc     6240

cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat     6300

aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa     6360

ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg     6420

caacatatgc catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat     6480

atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag     6540

atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta     6600

catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct     6660

cttctcttat gaagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct     6720

gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat     6780

aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc     6840

actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca     6900

gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc     6960

cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctaa     7020

acggccggcc atcgataccg tcgacctcga gggggggccc ggtacccagc ttttgttccc     7080

tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa     7140

attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct     7200

ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc     7260

agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg     7320

gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc     7380

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag     7440

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa     7500

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     7560

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     7620

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     7680

cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt     7740

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     7800

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     7860

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag     7920

agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg     7980

ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa     8040

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag     8100

gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact     8160

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa     8220

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt     8280

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag     8340

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca     8400

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc     8460

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt     8520

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg     8580

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca     8640

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg     8700

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     8760

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     8820

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     8880

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     8940

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     9000

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     9060

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     9120

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt     9180

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc     9240

cgcgcacatt tccccgaaaa gtgccac                                         9267


<210>  2
<211>  134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector and Construct Component, 5' end

<400>  2
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtg                                                        134


<210>  3
<211>  325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F1 ori

<400>  3
ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc       60

gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt      120

tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag      180

cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg      240

gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc      300

ttaatgcgcc gctacagggc gcgtc                                            325


<210>  4
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacZ alpha

<400>  4
ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct       60

attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg      120

gttttcccag tcacgacgt                                                   139


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13 fwd

<400>  5
tgtaaaacga cggccagtga gcgcgc                                            26


<210>  6
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7

<400>  6
gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac       60

tagtg                                                                   65


<210>  7
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BAMH1, p16 promoter

<400>  7
gatcc                                                                    5


<210>  8
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forprimer3, p16 promoter

<400>  8
gtgtaaagtc act                                                          13


<210>  9
<211>  2604
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16 promoter

<400>  9
cttttatagc tacatctgca tagatcccct gtatgaaagc atgtactacc tggataataa       60

tatctgtatt tttctgtagt aggaaatcag tgtagttttt aaaaccaaaa agtattgtta      120

ttaatctatc tttgatctca aacaatttca atgacctagt atagtgattt ctacggaaag      180

ccctgcaatt tactcaaagc agtttttaaa tattgtttta aaagtgtgtg tgtgtgtgtg      240

tgtgtgtgtg tgtgtgtgtg tggtgttaaa gtcattttca aacccctcac aatgtcttga      300

atgtgacatt tgagtcattt atggtaactt ataactcctt tgaagaagtt attcagaatt      360

gaggttccag acacacaaat gcacaataca ccatttttcc ttccagttaa caatcagagg      420

gcaacactta tttttaaagg aaaatcgact ccataaggga ctttataaag gggtagacat      480

aaaccagtat cagggataaa ctctccgttc ccctgtttaa cctaattttc ccagggccat      540

cctggaatac gaattttctc ttgaaataca gtcaaagaaa aagtggtagg ctacagagca      600

gaggaaacac tggacacagc gacccacccc agagtcactt cccttaatct aatgactagg      660

ttttttctga aagttatttt gttagaacac aggaactttt gcgaccacag tgatgctttt      720

agagggttga atcctcaaaa agaaaattaa tcgcaactag tagaagggag attacttatt      780

gattcttata acttctgcag gaatacacag ttatgagtta gggcaaagag aaaattgact      840

tttaatattc tctatcacta acatgagaga acatgtatgt gttccaaaat aatttttatt      900

tattgaaaac ccgctatata cctggatttt cacagaatat tcattactct ccaaaatggc      960

cttttctagg tgaattttat tttccttaca gacctcaaga agtttacata atttacttaa     1020

acctgaggag agagaacaaa gcctcagaaa atttacatag tttatttaaa ctaaactcag     1080

cttgcttggt agcagcttct aatcccagca gttaaagaga cagaagcagg gccaacctgg     1140

ggtataatat aaggtgagac tctcctttct ttctctctgt ctctgtctgt ctctgtctct     1200

gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtctcc tctctctctc tctctctctc     1260

tctctctctc tctctgtctc tctctccctc cccctccctc cctctccccc tcctctctcc     1320

ctccctctcc ctcccccccc cccacacatt tgaattcgtg gagttggtaa atgaggggtc     1380

agttctctgt ctgtctgtag ttttgtgtcc acaggatatg actgacattc tcaccacaca     1440

catacaaagt caaaaatagc tgtggccata taaagaatat ggggagagaa aattattcaa     1500

aatctgcaga aaataatgcc aggcctttaa tcctggcacc caggaggcag aagggagaca     1560

gagttctgag tttatgctga gttccaggag tggaagaaag ggccattgcc tttctggtga     1620

ggactgtctt tttaaatcct cccttctgtc cagtactggt aactctgccc aaagcgtgtt     1680

cttcttcctg cctcacaaga ttgcaaagac gtttttaacg aacaatttaa accggtgcaa     1740

cgtttatgcg cagcacacca actcatttaa acaaacaaca gccccataaa atagaaatac     1800

tttataagca gattgccctc cgatgacttc accccgtcac ttttttatag ttgtgtacag     1860

aatcctagca ctgatacagc aacatcagaa atgtttctgc aaatccttcg caaagattcg     1920

gatttcatac tgggcgtggt accctccaaa atgagttgtt tgagctaggg ttgttgggat     1980

ctcagcttgg cgaagttgta gctctttctt ctgaataaaa gatgacacaa ttttctgcta     2040

agatgttaaa taccttaagt ttcagtgtag tgatgaaaat taccctcctt cgtttttcta     2100

atacctgggt gttgcactgg ggaggaagga gagatttcga gaaggactag ttcactttct     2160

cagaagacac gtgtgcactt ctttgctgtg cgggtccaga aggagcccag cgtgtcaaag     2220

ggtgaccagg catgggggag gggtgttagc gtgggtagca ggcgggggct gtccgatcct     2280

ttagcgctgt ttcaacgccc agctctcctc ctgaaccctg catctcttct gtagtccggg     2340

ctccatccct ttcccctccc ccatccggag gtggggggaa cagcagtgtt ttcaggggtg     2400

ttcaattcat gctatattca gggcaaatag cgccacctat ggcgggctgt ggagccaggt     2460

caggagcaga gtgtggctcc cccccccccc cacaccatcc tcagaggaag gaaggaggga     2520

cccactggtc acacgactgg gcgattgggc gggcactgaa tctccgcgag gaaagcgaac     2580

tcgaggagag ccatcacgcg tagc                                            2604


<210>  10
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FKBP

<400>  10
atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg cgtccaagtc       60

gaaaccatta gtcccggcga tggcagaaca tttcctaaaa ggggacaaac atgtgtcgtc      120

cattatacag gcatgttgga ggacggcaaa aaggtggaca gtagtagaga tcgcaataaa      180

cctttcaaat tcatgttggg aaaacaagaa gtcattaggg gatgggagga gggcgtggct      240

caaatgtccg tcggccaacg cgctaagctc accatcagcc ccgactacgc atacggcgct      300

accggacatc ccggaattat tccccctcac gctaccttgg tgtttgacgt cgaactgttg      360

aagctcgaga ctagaggagt gcaggtggag actatctccc caggagacgg gcgcaccttc      420

cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa      480

gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg      540

atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact      600

atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc      660

actctcgtct tcgatgtgga gcttctaaaa ctggaaacta gt                         702


<210>  11
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Casp8

<400>  11
agtgaatcac agactttgga caaagtttac caaatgaaaa gcaaacctcg gggatactgt       60

ctgatcatca acaatcacaa ttttgcaaaa gcacgggaga aagtgcccaa acttcacagc      120

attagggaca ggaatggaac acacttggat gcaggggctt tgaccacgac ctttgaagag      180

cttcattttg agatcaagcc ccacgatgac tgcacagtag agcaaatcta tgagattttg      240

aaaatctacc aactcatgga ccacagtaac atggactgct tcatctgctg tatcctctcc      300

catggagaca agggcatcat ctatggcact gatggacagg aggcccccat ctatgagctg      360

acatctcagt tcactggttt gaagtgccct tcccttgctg gaaaacccaa agtgtttttt      420

attcaggctt gtcaggggga taactaccag aaaggtatac ctgttgagac tgattcagag      480

gagcaaccct atttagaaat ggatttatca tcacctcaaa cgagatatat cccggatgag      540

gctgactttc tgctggggat ggccactgtg aataactgtg tttcctaccg aaaccctgca      600

gagggaacct ggtacatcca gtcactttgc cagagcctga gagagcgatg tcctcgaggc      660

gatgatattc tcaccatcct gactgaagtg aactatgaag taagcaacaa ggatgacaag      720

aaaaacatgg ggaaacagat gcctcagcct actttcacac taagaaaaaa acttgtcttc      780

ccttctgat                                                              789


<210>  12
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Flag/Tag/Stop

<400>  12
gattacaagg atgacgacga taagtga                                           27


<210>  13
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' UTR

<400>  13
ggatc                                                                    5


<210>  14
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multiple cloning site (MluI, Pac1, Xho1, Pme1)

<400>  14
aacctcgagg aattcacgcg tttaattaac tcgaggttt                              39


<210>  15
<211>  1268
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRES, GFP

<400>  15
tcgaggtcga cggtatcgat aagcttgata tcgaattccg cccctctccc tccccccccc       60

ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat      120

tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct      180

tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg      240

tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc      300

tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg      360

tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg      420

tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga      480

aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt      540

agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa      600

aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg      660

gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc      720

ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc      780

ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc      840

ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa      900

ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc      960

gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc     1020

aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc     1080

tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac     1140

atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac     1200

ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac     1260

cccaacga                                                              1268


<210>  16
<211>  565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rabbit beta-globin PA

<400>  16
gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat       60

ggacgagctg tacaagtaaa gcggccgcga tctttttccc tctgccaaaa attatgggga      120

catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc      180

aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca      240

tttaaaacat cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct      300

gccatgaaca aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc      360

attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg      420

tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt      480

tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatga agatccctcg      540

acctgcagcc caagcttggc gtaat                                            565


<210>  17
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13-rev

<400>  17
catggtcata gctgtttcct gtgtga                                            26


<210>  18
<211>  285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacO

<400>  18
aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc       60

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc      120

cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc      180

cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc      240

atggctgact aatttttttt atttatgcag aggccgaggc cgcct                      285


<210>  19
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fse1, linker

<400>  19
aaacggccgg ccatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgt         58


<210>  20
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3

<400>  20
tccctttagt gagggttaat tgcgcgcttg gcgtaat                                37


<210>  21
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13-rev

<400>  21
catggtcata gctgtttcct gtgtga                                            26


<210>  22
<211>  362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacO

<400>  22
aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc       60

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc      120

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc      180

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt      240

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca      300

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa      360

aa                                                                     362


<210>  23
<211>  780
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ColE1 origin

<400>  23
ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg       60

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc      120

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc      180

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc      240

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg      300

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc      360

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga      420

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc      480

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac      540

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg      600

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc      660

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa      720

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta      780


<210>  24
<211>  986
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AmpR

<400>  24
ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt       60

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag      120

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca      180

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc      240

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt      300

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag      360

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt      420

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat      480

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt      540

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc      600

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat      660

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag      720

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt      780

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg      840

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta      900

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc      960

gcgcacattt ccccgaaaag tgccac                                           986


<210>  25
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tubulin-A forward primer

<400>  25
cttcgtctcc gccatcag                                                     18


<210>  26
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tubulin-A reverse primer

<400>  26
ttgccaatct ggacacca                                                     18


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-6 forward primer

<400>  27
gcccagctat gaactccttc t                                                 21


<210>  28
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-6 reverse primer

<400>  28
gaaggcagca ggcaacac                                                     18


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-8 forward primer

<400>  29
agacagcaga gcacacaagc                                                   20


<210>  30
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-8 reverse primer

<400>  30
atggttcctt ccggtggt                                                     18


<210>  31
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MMP-3 foward primer

<400>  31
caaaacatat ttctttgtag aggacaa                                           27


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MMP-3 reverse primer

<400>  32
ttcagctatt tgcttgggaa a                                                 21


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GR forward primer

<400>  33
gaaagccacg ctcccttc                                                     18


<210>  34
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GR reverse primer

<400>  34
agacttaggt gaaactggaa ttgct                                             25


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-1alpha forward primer

<400>  35
ggttgagttt aagccaatcc a                                                 21


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-1alpha reverse primer

<400>  36
tgctgaccta ggcttgatga                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IkappaBalpha forward primer

<400>  37
ggtgctgatg tcaatgctca                                                   20


<210>  38
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IkappaBalpha reverese primer

<400>  38
acaccaggtc aggatttt                                                     18


<210> 39
<211> 2664
<212> DNA
<213> Artificial Sequence

<220> 
<223> Exemplary 3MR transgene sequence

<400> 39
atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg      60
tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag     120
aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg     180
aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga     240
atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac     300
ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac     360
tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc     420
gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag     480
gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc     540
ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct     600
gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct     660
cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac     720
aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg     780
ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag     840
gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag     900
agcttcgtgg agcgcgtgct gaagaacgag cagctcgaga attctcacgc gtctgcagga     960
tatcaagctt ccaccatggc ctcctccgag gacgtcatca agttcatgcg cttcaaggtg    1020
cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc    1080
ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc    1140
gcctgggaca tcctgtcccc tcagttccag tacggctcca aggcctacgt gaagcacccc    1200
gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg    1260
atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc    1320
gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg    1380
cagaagaaga ccatgggctg ggaggcctcc accgagagga tgtaccccga ggacggcgcc    1440
ctgaagggcg agatcaagat gaggctgaag ctgaaggacg gcggccacta cgacgccgag    1500
gtcaagacca cctacatggc caagaagccc gtgcagctgc ccggcgccta caagaccgac    1560
atcaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc    1620
gccgagggcc gccactccac cggcgccacc gcgggcccgg gatccgccac catgcccacg    1680
ctactgcggg tttatataga cggtccccac gggatgggga aaaccaccac caccacgcaa    1740
ctgctggtgg ccctgggttc gcgcgacgat atcgtctacg tacccgagcc gatgacttac    1800
tggcgggtgc tgggggcttc cgagacaatc gcgaacatct acaccacaca acaccgcctc    1860
gaccagggtg agatatcggc cggggacgcg gcggtggtaa tgacaagcgc ccagataaca    1920
atgccttatg ccgtgaccga cgccgttctg gctcctcata tcggggggga ggctgggagc    1980
tcacatgccc cgcccccggc cctcaccatc ttcctcgacc gccatcccat cgccttcatg    2040
ctgtgctacc cggccgcgcg gtaccttatg ggcagcatga ccccccaggc cgtgctggcg    2100
ttcgtggccc tcatcccgcc gaccttgccc ggcaccaaca tcgtgcttgg ggcccttccg    2160
gaggacagac acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg gctggacctg    2220
gctatgctgg ctgcgattcg ccgcgtttac gggctacttg ccaatacggt gcggtatctg    2280
cagtgcggcg ggtcgtggcg ggaggactgg ggacagcttt cggggacggc cgtgccgccc    2340
cagggtgccg agccccagag caacgcgggc ccacgacccc atatcgggga cacgttattt    2400
accctgtttc gggcccccga gttgatggcc cccaacggcg acctgtataa cgtgtttgcc    2460
tgggccttgg acgtcttggc caaacgcctc cgttccatgc acgtctttat cctggattac    2520
gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg gatggtccag    2580
acccacgtca ccacccccgg ctccataccg acgatatgcg acctggcgcg cacgtttgcc    2640
cgggagatgg gggaggctaa ctga                                           2664

