                         SEQUENCE LISTING

<110>  New York University
       Torres, Victor  J.
       Alonzo, Francis  III
       Reyes-Robles, Tamara
 
<120>  CELLULAR FACTORS INVOLVED IN THE CYTOTOXICITY OF STAPHYLOCOCCUS 
       AUREUS LEUKOCIDINS: NOVEL THERAPEUTIC TARGETS

<130>  29527.1501 (TOR01-05PCT)

<150>  61836516
<151>  2013-06-18

<160>  56    

<170>  PatentIn version 3.5

<210>  1
<211>  72
<212>  PRT
<213>  artificial

<220>
<223>  CXCL8(3-74)K11R/G31P

<400>  1

Thr Glu Leu Arg Cys Gln Cys Ile Arg Thr His Ser Thr Pro Phe His 
1               5                   10                  15      


Pro Lys Phe Ile Lys Glu Leu Arg Val Ile Glu Ser Pro Pro His Cys 
            20                  25                  30          


Glu Asn Ser Glu Ile Ile Val Lys Leu Thr Asn Gly Asn Glu Val Cys 
        35                  40                  45              


Leu Asn Pro Lys Glu Lys Trp Val Gln Lys Val Val Gln Val Phe Val 
    50                  55                  60                  


Lys Arg Ala Glu Lys Gln Asp Pro 
65                  70          


<210>  2
<211>  72
<212>  PRT
<213>  artificial

<220>
<223>  CXCL8(3-74)K11R/G31P/P32G

<400>  2

Thr Glu Leu Arg Cys Gln Cys Ile Arg Thr His Ser Thr Pro Phe His 
1               5                   10                  15      


Pro Lys Phe Ile Lys Glu Leu Arg Val Ile Glu Ser Gly Gly His Cys 
            20                  25                  30          


Glu Asn Ser Glu Ile Ile Val Lys Leu Thr Asn Gly Asn Glu Val Cys 
        35                  40                  45              


Leu Asn Pro Lys Glu Lys Trp Val Gln Lys Val Val Gln Val Phe Val 
    50                  55                  60                  


Lys Arg Ala Glu Lys Gln Asp Pro 
65                  70          


<210>  3
<211>  72
<212>  PRT
<213>  artificial

<220>
<223>  CXCL8(3-74)K11R/T12S/H13F/G31P

<400>  3

Thr Glu Leu Arg Cys Gln Cys Ile Arg Ser Pro Ser Thr Pro Phe His 
1               5                   10                  15      


Pro Lys Phe Ile Lys Glu Leu Arg Val Ile Glu Ser Pro Pro His Cys 
            20                  25                  30          


Glu Asn Ser Glu Ile Ile Val Lys Leu Thr Asn Gly Asn Glu Val Cys 
        35                  40                  45              


Leu Asn Pro Lys Glu Lys Trp Val Gln Lys Val Val Gln Val Phe Val 
    50                  55                  60                  


Lys Arg Ala Glu Lys Gln Asp Pro 
65                  70          


<210>  4
<211>  283
<212>  PRT
<213>  Staphylococcus aureus

<400>  4

Asn Thr Asn Ile Glu Asn Ile Gly Asp Gly Ala Glu Val Ile Lys Arg 
1               5                   10                  15      


Thr Glu Asp Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys 
    50                  55                  60                  


Gly Ser Gly Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr 
65                  70                  75                  80  


Asn Ile Gly Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr 
                85                  90                  95      


Leu Pro Lys Asn Lys Ile Glu Thr Thr Asp Val Gly Gln Thr Leu Gly 
            100                 105                 110         


Tyr Asn Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Asn 
        115                 120                 125             


Gly Ser Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Thr Gln Lys Ser Tyr 
    130                 135                 140                 


Val Ser Glu Val Asp Lys Gln Asn Ser Lys Ser Val Lys Trp Gly Val 
145                 150                 155                 160 


Lys Ala Asn Glu Phe Val Thr Pro Asp Gly Lys Lys Ser Ala His Asp 
                165                 170                 175     


Arg Tyr Leu Phe Val Gln Ser Pro Asn Gly Pro Thr Gly Ser Ala Arg 
            180                 185                 190         


Glu Tyr Phe Ala Pro Asp Asn Gln Leu Pro Pro Leu Val Gln Ser Gly 
        195                 200                 205             


Phe Asn Pro Ser Phe Ile Thr Thr Leu Ser His Glu Lys Gly Ser Ser 
    210                 215                 220                 


Asp Thr Ser Glu Phe Glu Ile Ser Tyr Gly Arg Asn Leu Asp Ile Thr 
225                 230                 235                 240 


Tyr Ala Thr Leu Phe Pro Arg Thr Gly Ile Tyr Ala Glu Arg Lys His 
                245                 250                 255     


Asn Ala Phe Val Asn Arg Asn Phe Val Val Arg Tyr Glu Val Asn Trp 
            260                 265                 270         


Lys Thr His Glu Ile Lys Val Lys Gly His Asn 
        275                 280             


<210>  5
<211>  15
<212>  PRT
<213>  Staphylococcus aureus

<400>  5

Gln Ser Pro Asn Gly Pro Thr Gly Ser Ala Arg Glu Tyr Phe Ala 
1               5                   10                  15  


<210>  6
<211>  280
<212>  PRT
<213>  Staphylococcus aureus

<400>  6

Glu Asn Lys Ile Glu Asp Ile Gly Gln Gly Ala Glu Ile Ile Lys Arg 
1               5                   10                  15      


Thr Gln Asp Ile Thr Ser Lys Arg Leu Ala Ile Thr Gln Asn Ile Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Val Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Ser Ser Arg Thr Thr Tyr Ser Asp Leu Lys 
    50                  55                  60                  


Lys Tyr Pro Tyr Ile Lys Arg Met Ile Trp Pro Phe Gln Tyr Asn Ile 
65                  70                  75                  80  


Ser Leu Lys Thr Lys Asp Ser Asn Val Asp Leu Ile Asn Tyr Leu Pro 
                85                  90                  95      


Lys Asn Lys Ile Asp Ser Ala Asp Val Ser Gln Lys Leu Gly Tyr Asn 
            100                 105                 110         


Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Ser Gly Ser 
        115                 120                 125             


Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Asn Gln Lys Asn Tyr Val Thr 
    130                 135                 140                 


Glu Val Glu Ser Gln Asn Ser Lys Gly Val Lys Trp Gly Val Lys Ala 
145                 150                 155                 160 


Asn Ser Phe Val Thr Pro Asn Gly Gln Val Ser Ala Tyr Asp Gln Tyr 
                165                 170                 175     


Leu Phe Ala Gln Asp Pro Thr Gly Pro Ala Ala Arg Asp Tyr Phe Val 
            180                 185                 190         


Pro Asp Asn Gln Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro Ser 
        195                 200                 205             


Phe Ile Thr Thr Leu Ser His Glu Arg Gly Lys Gly Asp Lys Ser Glu 
    210                 215                 220                 


Phe Glu Ile Thr Tyr Gly Arg Asn Met Asp Ala Thr Tyr Ala Tyr Val 
225                 230                 235                 240 


Thr Arg His Arg Leu Ala Val Asp Arg Lys His Asp Ala Phe Lys Asn 
                245                 250                 255     


Arg Asn Val Thr Val Lys Tyr Glu Val Asn Trp Lys Thr His Glu Val 
            260                 265                 270         


Lys Ile Lys Ser Ile Thr Pro Lys 
        275                 280 


<210>  7
<211>  13
<212>  PRT
<213>  Staphylococcus aureus

<400>  7

Gln Asp Pro Thr Gly Pro Ala Ala Arg Asp Tyr Phe Val 
1               5                   10              


<210>  8
<211>  282
<212>  PRT
<213>  artificial

<220>
<223>  LukELukS-DR4

<400>  8

Asn Thr Asn Ile Glu Asn Ile Gly Asp Gly Ala Glu Val Ile Lys Arg 
1               5                   10                  15      


Thr Glu Asp Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys 
    50                  55                  60                  


Gly Ser Gly Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr 
65                  70                  75                  80  


Asn Ile Gly Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr 
                85                  90                  95      


Leu Pro Lys Asn Lys Ile Glu Thr Thr Asp Val Gly Gln Thr Leu Gly 
            100                 105                 110         


Tyr Asn Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Asn 
        115                 120                 125             


Gly Ser Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Thr Gln Lys Ser Tyr 
    130                 135                 140                 


Val Ser Glu Val Asp Lys Gln Asn Ser Lys Ser Val Lys Trp Gly Val 
145                 150                 155                 160 


Lys Ala Asn Glu Phe Val Thr Pro Asp Gly Lys Lys Ser Ala His Asp 
                165                 170                 175     


Arg Tyr Leu Phe Val Gly Tyr Lys Pro Tyr Ser Gln Asn Pro Arg Asp 
            180                 185                 190         


Tyr Phe Val Pro Asp Asn Gln Leu Pro Pro Leu Val Gln Ser Gly Phe 
        195                 200                 205             


Asn Pro Ser Phe Ile Thr Thr Leu Ser His Glu Lys Gly Ser Ser Asp 
    210                 215                 220                 


Thr Ser Glu Phe Glu Ile Ser Tyr Gly Arg Asn Leu Asp Ile Thr Tyr 
225                 230                 235                 240 


Ala Thr Leu Phe Pro Arg Thr Gly Ile Tyr Ala Glu Arg Lys His Asn 
                245                 250                 255     


Ala Phe Val Asn Arg Asn Phe Val Val Arg Tyr Glu Val Asn Trp Lys 
            260                 265                 270         


Thr His Glu Ile Lys Val Lys Gly His Asn 
        275                 280         


<210>  9
<211>  243
<212>  PRT
<213>  artificial

<220>
<223>  LukE20-263 LukS-DR4

<400>  9

Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln Phe Asp Phe 
1               5                   10                  15      


Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val Lys Met Gln 
            20                  25                  30          


Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys Gly Ser Gly 
        35                  40                  45              


Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr Asn Ile Gly 
    50                  55                  60                  


Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr Leu Pro Lys 
65                  70                  75                  80  


Asn Lys Ile Glu Thr Thr Asp Val Gly Gln Thr Leu Gly Tyr Asn Ile 
                85                  90                  95      


Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Asn Gly Ser Phe 
            100                 105                 110         


Asn Tyr Ser Lys Thr Ile Ser Tyr Thr Gln Lys Ser Tyr Val Ser Glu 
        115                 120                 125             


Val Asp Lys Gln Asn Ser Lys Ser Val Lys Trp Gly Val Lys Ala Asn 
    130                 135                 140                 


Glu Phe Val Thr Pro Asp Gly Lys Lys Ser Ala His Asp Arg Tyr Leu 
145                 150                 155                 160 


Phe Val Gly Tyr Lys Pro Tyr Ser Gln Asn Pro Arg Asp Tyr Phe Val 
                165                 170                 175     


Pro Asp Asn Gln Leu Pro Pro Leu Val Gln Ser Gly Phe Asn Pro Ser 
            180                 185                 190         


Phe Ile Thr Thr Leu Ser His Glu Lys Gly Ser Ser Asp Thr Ser Glu 
        195                 200                 205             


Phe Glu Ile Ser Tyr Gly Arg Asn Leu Asp Ile Thr Tyr Ala Thr Leu 
    210                 215                 220                 


Phe Pro Arg Thr Gly Ile Tyr Ala Glu Arg Lys His Asn Ala Phe Val 
225                 230                 235                 240 


Asn Arg Asn 
            


<210>  10
<211>  283
<212>  PRT
<213>  artificial

<220>
<223>  LukEP184A,G186A,P187A

<400>  10

Asn Thr Asn Ile Glu Asn Ile Gly Asp Gly Ala Glu Val Ile Lys Arg 
1               5                   10                  15      


Thr Glu Asp Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys 
    50                  55                  60                  


Gly Ser Gly Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr 
65                  70                  75                  80  


Asn Ile Gly Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr 
                85                  90                  95      


Leu Pro Lys Asn Lys Ile Glu Thr Thr Asp Val Gly Gln Thr Leu Gly 
            100                 105                 110         


Tyr Asn Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Asn 
        115                 120                 125             


Gly Ser Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Thr Gln Lys Ser Tyr 
    130                 135                 140                 


Val Ser Glu Val Asp Lys Gln Asn Ser Lys Ser Val Lys Trp Gly Val 
145                 150                 155                 160 


Lys Ala Asn Glu Phe Val Thr Pro Asp Gly Lys Lys Ser Ala His Asp 
                165                 170                 175     


Arg Tyr Leu Phe Val Gln Ser Ala Asn Ala Ala Thr Gly Ser Ala Arg 
            180                 185                 190         


Glu Tyr Phe Ala Pro Asp Asn Gln Leu Pro Pro Leu Val Gln Ser Gly 
        195                 200                 205             


Phe Asn Pro Ser Phe Ile Thr Thr Leu Ser His Glu Lys Gly Ser Ser 
    210                 215                 220                 


Asp Thr Ser Glu Phe Glu Ile Ser Tyr Gly Arg Asn Leu Asp Ile Thr 
225                 230                 235                 240 


Tyr Ala Thr Leu Phe Pro Arg Thr Gly Ile Tyr Ala Glu Arg Lys His 
                245                 250                 255     


Asn Ala Phe Val Asn Arg Asn Phe Val Val Arg Tyr Glu Val Asn Trp 
            260                 265                 270         


Lys Thr His Glu Ile Lys Val Lys Gly His Asn 
        275                 280             


<210>  11
<211>  283
<212>  PRT
<213>  artificial

<220>
<223>  LukEP184A,G186A,P187A,G189A

<400>  11

Asn Thr Asn Ile Glu Asn Ile Gly Asp Gly Ala Glu Val Ile Lys Arg 
1               5                   10                  15      


Thr Glu Asp Val Ser Ser Lys Lys Trp Gly Val Thr Gln Asn Val Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Ile Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Asn Ser Arg Thr Ser Phe Ser Asp Val Lys 
    50                  55                  60                  


Gly Ser Gly Tyr Glu Leu Thr Lys Arg Met Ile Trp Pro Phe Gln Tyr 
65                  70                  75                  80  


Asn Ile Gly Leu Thr Thr Lys Asp Pro Asn Val Ser Leu Ile Asn Tyr 
                85                  90                  95      


Leu Pro Lys Asn Lys Ile Glu Thr Thr Asp Val Gly Gln Thr Leu Gly 
            100                 105                 110         


Tyr Asn Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Asn 
        115                 120                 125             


Gly Ser Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Thr Gln Lys Ser Tyr 
    130                 135                 140                 


Val Ser Glu Val Asp Lys Gln Asn Ser Lys Ser Val Lys Trp Gly Val 
145                 150                 155                 160 


Lys Ala Asn Glu Phe Val Thr Pro Asp Gly Lys Lys Ser Ala His Asp 
                165                 170                 175     


Arg Tyr Leu Phe Val Gln Ser Ala Asn Ala Ala Thr Ala Ser Ala Arg 
            180                 185                 190         


Glu Tyr Phe Ala Pro Asp Asn Gln Leu Pro Pro Leu Val Gln Ser Gly 
        195                 200                 205             


Phe Asn Pro Ser Phe Ile Thr Thr Leu Ser His Glu Lys Gly Ser Ser 
    210                 215                 220                 


Asp Thr Ser Glu Phe Glu Ile Ser Tyr Gly Arg Asn Leu Asp Ile Thr 
225                 230                 235                 240 


Tyr Ala Thr Leu Phe Pro Arg Thr Gly Ile Tyr Ala Glu Arg Lys His 
                245                 250                 255     


Asn Ala Phe Val Asn Arg Asn Phe Val Val Arg Tyr Glu Val Asn Trp 
            260                 265                 270         


Lys Thr His Glu Ile Lys Val Lys Gly His Asn 
        275                 280             


<210>  12
<211>  301
<212>  PRT
<213>  Staphylococcus aureus

<400>  12

Ala Gln His Ile Thr Pro Val Ser Glu Lys Lys Val Asp Asp Lys Ile 
1               5                   10                  15      


Thr Leu Tyr Lys Thr Thr Ala Thr Ser Asp Asn Asp Lys Leu Asn Ile 
            20                  25                  30          


Ser Gln Ile Leu Thr Phe Asn Phe Ile Lys Asp Lys Ser Tyr Asp Lys 
        35                  40                  45              


Asp Thr Leu Val Leu Lys Ala Ala Gly Asn Ile Asn Ser Gly Tyr Lys 
    50                  55                  60                  


Lys Pro Asn Pro Lys Asp Tyr Asn Tyr Ser Gln Phe Tyr Trp Gly Gly 
65                  70                  75                  80  


Lys Tyr Asn Val Ser Val Ser Ser Glu Ser Asn Asp Ala Val Asn Val 
                85                  90                  95      


Val Asp Tyr Ala Pro Lys Asn Gln Asn Glu Glu Phe Gln Val Gln Gln 
            100                 105                 110         


Thr Leu Gly Tyr Ser Tyr Gly Gly Asp Ile Asn Ile Ser Asn Gly Leu 
        115                 120                 125             


Ser Gly Gly Leu Asn Gly Ser Lys Ser Phe Ser Glu Thr Ile Asn Tyr 
    130                 135                 140                 


Lys Gln Glu Ser Tyr Arg Thr Thr Ile Asp Arg Lys Thr Asn His Lys 
145                 150                 155                 160 


Ser Ile Gly Trp Gly Val Glu Ala His Lys Ile Met Asn Asn Gly Trp 
                165                 170                 175     


Gly Pro Tyr Gly Arg Asp Ser Tyr Asp Pro Thr Tyr Gly Asn Glu Leu 
            180                 185                 190         


Phe Leu Gly Gly Arg Gln Ser Ser Ser Asn Ala Gly Gln Asn Phe Leu 
        195                 200                 205             


Pro Thr His Gln Met Pro Leu Leu Ala Arg Gly Asn Phe Asn Pro Glu 
    210                 215                 220                 


Phe Ile Ser Val Leu Ser His Lys Gln Asn Asp Thr Lys Lys Ser Lys 
225                 230                 235                 240 


Ile Lys Val Thr Tyr Gln Arg Glu Met Asp Arg Tyr Thr Asn Gln Trp 
                245                 250                 255     


Asn Arg Leu His Trp Val Gly Asn Asn Tyr Lys Asn Gln Asn Thr Val 
            260                 265                 270         


Thr Phe Thr Ser Thr Tyr Glu Val Asp Trp Gln Asn His Thr Val Lys 
        275                 280                 285             


Leu Ile Gly Thr Asp Ser Lys Glu Thr Asn Pro Gly Val 
    290                 295                 300     


<210>  13
<211>  281
<212>  PRT
<213>  artificial

<220>
<223>  HlgALukS-DR4

<400>  13

Glu Asn Lys Ile Glu Asp Ile Gly Gln Gly Ala Glu Ile Ile Lys Arg 
1               5                   10                  15      


Thr Gln Asp Ile Thr Ser Lys Arg Leu Ala Ile Thr Gln Asn Ile Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Val Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Ser Ser Arg Thr Thr Tyr Ser Asp Leu Lys 
    50                  55                  60                  


Lys Tyr Pro Tyr Ile Lys Arg Met Ile Trp Pro Phe Gln Tyr Asn Ile 
65                  70                  75                  80  


Ser Leu Lys Thr Lys Asp Ser Asn Val Asp Leu Ile Asn Tyr Leu Pro 
                85                  90                  95      


Lys Asn Lys Ile Asp Ser Ala Asp Val Ser Gln Lys Leu Gly Tyr Asn 
            100                 105                 110         


Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Ser Gly Ser 
        115                 120                 125             


Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Asn Gln Lys Asn Tyr Val Thr 
    130                 135                 140                 


Glu Val Glu Ser Gln Asn Ser Lys Gly Val Lys Trp Gly Val Lys Ala 
145                 150                 155                 160 


Asn Ser Phe Val Thr Pro Asn Gly Gln Val Ser Ala Tyr Asp Gln Tyr 
                165                 170                 175     


Leu Phe Ala Gly Tyr Lys Pro Tyr Ser Gln Asn Pro Arg Asp Tyr Phe 
            180                 185                 190         


Val Pro Asp Asn Gln Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro 
        195                 200                 205             


Ser Phe Ile Thr Thr Leu Ser His Glu Arg Gly Lys Gly Asp Lys Ser 
    210                 215                 220                 


Glu Phe Glu Ile Thr Tyr Gly Arg Asn Met Asp Ala Thr Tyr Ala Tyr 
225                 230                 235                 240 


Val Thr Arg His Arg Leu Ala Val Asp Arg Lys His Asp Ala Phe Lys 
                245                 250                 255     


Asn Arg Asn Val Thr Val Lys Tyr Glu Val Asn Trp Lys Thr His Glu 
            260                 265                 270         


Val Lys Ile Lys Ser Ile Thr Pro Lys 
        275                 280     


<210>  14
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  HlgA20-258 LukS-DR

<400>  14

Ile Thr Ser Lys Arg Leu Ala Ile Thr Gln Asn Ile Gln Phe Asp Phe 
1               5                   10                  15      


Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Val Val Lys Met Gln 
            20                  25                  30          


Gly Phe Ile Ser Ser Arg Thr Thr Tyr Ser Asp Leu Lys Lys Tyr Pro 
        35                  40                  45              


Tyr Ile Lys Arg Met Ile Trp Pro Phe Gln Tyr Asn Ile Ser Leu Lys 
    50                  55                  60                  


Thr Lys Asp Ser Asn Val Asp Leu Ile Asn Tyr Leu Pro Lys Asn Lys 
65                  70                  75                  80  


Ile Asp Ser Ala Asp Val Ser Gln Lys Leu Gly Tyr Asn Ile Gly Gly 
                85                  90                  95      


Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Ser Gly Ser Phe Asn Tyr 
            100                 105                 110         


Ser Lys Thr Ile Ser Tyr Asn Gln Lys Asn Tyr Val Thr Glu Val Glu 
        115                 120                 125             


Ser Gln Asn Ser Lys Gly Val Lys Trp Gly Val Lys Ala Asn Ser Phe 
    130                 135                 140                 


Val Thr Pro Asn Gly Gln Val Ser Ala Tyr Asp Gln Tyr Leu Phe Ala 
145                 150                 155                 160 


Gly Tyr Lys Pro Tyr Ser Gln Asn Pro Arg Asp Tyr Phe Val Pro Asp 
                165                 170                 175     


Asn Gln Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro Ser Phe Ile 
            180                 185                 190         


Thr Thr Leu Ser His Glu Arg Gly Lys Gly Asp Lys Ser Glu Phe Glu 
        195                 200                 205             


Ile Thr Tyr Gly Arg Asn Met Asp Ala Thr Tyr Ala Tyr Val Thr Arg 
    210                 215                 220                 


His Arg Leu Ala Val Asp Arg Lys His Asp Ala Phe Lys Asn Arg Asn 
225                 230                 235                 240 


<210>  15
<211>  280
<212>  PRT
<213>  artificial

<220>
<223>  HlgAP182A,G184A,P185A

<400>  15

Glu Asn Lys Ile Glu Asp Ile Gly Gln Gly Ala Glu Ile Ile Lys Arg 
1               5                   10                  15      


Thr Gln Asp Ile Thr Ser Lys Arg Leu Ala Ile Thr Gln Asn Ile Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Val Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Ser Ser Arg Thr Thr Tyr Ser Asp Leu Lys 
    50                  55                  60                  


Lys Tyr Pro Tyr Ile Lys Arg Met Ile Trp Pro Phe Gln Tyr Asn Ile 
65                  70                  75                  80  


Ser Leu Lys Thr Lys Asp Ser Asn Val Asp Leu Ile Asn Tyr Leu Pro 
                85                  90                  95      


Lys Asn Lys Ile Asp Ser Ala Asp Val Ser Gln Lys Leu Gly Tyr Asn 
            100                 105                 110         


Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Ser Gly Ser 
        115                 120                 125             


Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Asn Gln Lys Asn Tyr Val Thr 
    130                 135                 140                 


Glu Val Glu Ser Gln Asn Ser Lys Gly Val Lys Trp Gly Val Lys Ala 
145                 150                 155                 160 


Asn Ser Phe Val Thr Pro Asn Gly Gln Val Ser Ala Tyr Asp Gln Tyr 
                165                 170                 175     


Leu Phe Ala Gln Asp Ala Thr Ala Ala Ala Ala Arg Asp Tyr Phe Val 
            180                 185                 190         


Pro Asp Asn Gln Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro Ser 
        195                 200                 205             


Phe Ile Thr Thr Leu Ser His Glu Arg Gly Lys Gly Asp Lys Ser Glu 
    210                 215                 220                 


Phe Glu Ile Thr Tyr Gly Arg Asn Met Asp Ala Thr Tyr Ala Tyr Val 
225                 230                 235                 240 


Thr Arg His Arg Leu Ala Val Asp Arg Lys His Asp Ala Phe Lys Asn 
                245                 250                 255     


Arg Asn Val Thr Val Lys Tyr Glu Val Asn Trp Lys Thr His Glu Val 
            260                 265                 270         


Lys Ile Lys Ser Ile Thr Pro Lys 
        275                 280 


<210>  16
<211>  299
<212>  PRT
<213>  artificial

<220>
<223>  HlgB

<400>  16

Glu Gly Lys Ile Thr Pro Val Ser Val Lys Lys Val Asp Asp Lys Val 
1               5                   10                  15      


Thr Leu Tyr Lys Thr Thr Ala Thr Ala Asp Ser Asp Lys Phe Lys Ile 
            20                  25                  30          


Ser Gln Ile Leu Thr Phe Asn Phe Ile Lys Asp Lys Ser Tyr Asp Lys 
        35                  40                  45              


Asp Thr Leu Val Leu Lys Ala Thr Gly Asn Ile Asn Ser Gly Phe Val 
    50                  55                  60                  


Lys Pro Asn Pro Asn Asp Tyr Asp Phe Ser Lys Leu Tyr Trp Gly Ala 
65                  70                  75                  80  


Lys Tyr Asn Val Ser Ile Ser Ser Gln Ser Asn Asp Ser Val Asn Val 
                85                  90                  95      


Val Asp Tyr Ala Pro Lys Asn Gln Asn Glu Glu Phe Gln Val Gln Asn 
            100                 105                 110         


Thr Leu Gly Tyr Thr Phe Gly Gly Asp Ile Ser Ile Ser Asn Gly Leu 
        115                 120                 125             


Ser Gly Gly Leu Asn Gly Asn Thr Ala Phe Ser Glu Thr Ile Asn Tyr 
    130                 135                 140                 


Lys Gln Glu Ser Tyr Arg Thr Thr Leu Ser Arg Asn Thr Asn Tyr Lys 
145                 150                 155                 160 


Asn Val Gly Trp Gly Val Glu Ala His Lys Ile Met Asn Asn Gly Trp 
                165                 170                 175     


Gly Pro Tyr Gly Arg Asp Ser Phe His Pro Thr Tyr Gly Asn Glu Leu 
            180                 185                 190         


Phe Leu Ala Gly Arg Gln Ser Ser Ala Tyr Ala Gly Gln Asn Phe Ile 
        195                 200                 205             


Ala Gln His Gln Met Pro Leu Leu Ser Arg Ser Asn Phe Asn Pro Glu 
    210                 215                 220                 


Phe Leu Ser Val Leu Ser His Arg Gln Asp Gly Ala Lys Lys Ser Lys 
225                 230                 235                 240 


Ile Thr Val Thr Tyr Gln Arg Glu Met Asp Leu Tyr Gln Ile Arg Trp 
                245                 250                 255     


Asn Gly Phe Tyr Trp Ala Gly Ala Asn Tyr Lys Asn Phe Lys Thr Arg 
            260                 265                 270         


Thr Phe Lys Ser Thr Tyr Glu Ile Asp Trp Glu Asn His Lys Val Lys 
        275                 280                 285             


Leu Leu Asp Thr Lys Glu Thr Glu Asn Asn Lys 
    290                 295                 


<210>  17
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  17
ccccctcgag aatactaata ttgaaaatat tggtgatg                               38


<210>  18
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  18
gtaataagta gtctttgaat taataaaacc ttgcatttta ac                          42


<210>  19
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  19
catataaaag caatgaggtg gccattccaa tataatatag                             40


<210>  20
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  20
tcaaagacta cttattacaa ttacaaaaac acagatcata taaaagcaat gagg             54


<210>  21
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  21
cctcattgct tttatatgat ctgtgttttt gtaattgtaa taagtagtct ttga             54


<210>  22
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  22
gttttgttga ttataactaa ttgttttaga ataattaaat g                           41


<210>  23
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  23
gaacgtcaaa attcaaaatc tgttaaatgg ggtg                                   34


<210>  24
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  24
agttataatc aacaaaacta tatcagtgaa gtagaacgtc aaaattca                    48


<210>  25
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  25
tgaattttga cgttctactt cactgatata gttttgttga ttataact                    48


<210>  26
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  26
gtgataaatg aattagcttt aacaccccat ttaac                                  35


<210>  27
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  27
gtaaaatgtc tggacatgat ccaaatttat ttgttgg                                37


<210>  28
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  28
gctaattcat ttatcacatc attaggtaaa atgtctggac atgat                       45


<210>  29
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  29
atcatgtcca gacattttac ctaatgatgt gataaatgaa ttagc                       45


<210>  30
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  30
ctatatggtt tatatccaac aaataaatat ctatcatgcg caga                        44


<210>  31
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  31
gactattttg ttccagacaa tcaattgcca cctttagttc aaag                        44


<210>  32
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  32
tgttggatat aaaccatata gtcaaaatcc gagagactat tttgttccag ac               52


<210>  33
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  33
gtctggaaca aaatagtctc tcggattttg actatatggt ttatatccaa ca               52


<210>  34
<211>  43
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  34
catgagtaac atccatattt ctaccatatg aaatttcaaa ttc                         43


<210>  35
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  35
gagactattt tgttccagat aatcaattgc cacctttag                              39


<210>  36
<211>  43
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  36
gaatttgaaa tttcatatgg tagaaatatg gatgttactc atg                         43


<210>  37
<211>  43
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  37
caaagtttct atttacaaat gcattgtgta ttctagatcc ttc                         43


<210>  38
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  38
ccccggatcc ttaattatgt cctttcactt taatttcg                               38


<210>  39
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  39
ccccctgcag gataggtgag atgcatacac aac                                    33


<210>  40
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  40
ccccggatcc ttatactcca ggattagttt ctttag                                 36


<210>  41
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  41
caatattttc aatattagta tttgctctag attcttgaat cggaga                      46


<210>  42
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  42
tctccgattc aagaatctag agcaaatact aatattgaaa atattg                      46


<210>  43
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  43
actaattttt tcattttcat attaattatg tcctttcact t                           41


<210>  44
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  44
aagtgaaagg acataattaa tatgaaaatg aaaaaattag t                           41


<210>  45
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  45
cccggatcca atactaatat tgaaaatatt ggtgatg                                37


<210>  46
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  46
cccctgcagt tatactccag gattagtttc tttag                                  35


<210>  47
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  47
cccggatcca atactaatat tgaaaatatt ggtgatg                                37


<210>  48
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  48
cccggatccg aaaataagat agaagatatc ggcc                                   34


<210>  49
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  49
cccctgcagt taattatgtc ctttcacttt aatttcg                                37


<210>  50
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  50
gcaaaatatt ctcttgctga tgctgttgct gcatttgcac tttgtacgaa taaatatc         58


<210>  51
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  51
gatatttatt cgtacaaagt gcaaatgcag caacagcatc agcaagagaa tattttgc         58


<210>  52
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  52
cccctgcagt tacttaggtg tgatgctttt aatttttac                              39


<210>  53
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  53
ggatataaac catatagtca aaatccgaga gactattttg ttccagataa tcaactacct       60

cc                                                                      62


<210>  54
<211>  66
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  54
aacaaaatag tctctcggat tttgactata tggtttatat cctgcaaata agtattgatc       60

atatgc                                                                  66


<210>  55
<211>  65
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  55
caaagtccaa atggtccaac aggttcagca agagaatatt ttgctccaga taatcaacta       60

cctcc                                                                   65


<210>  56
<211>  69
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  56
agcaaaatat tctcttgctg aacctgttgg accatttgga ctttgtgcaa ataagtattg       60

atcatatgc                                                               69


