                         SEQUENCE LISTING

<110>  APSE, LLC
       Arhancet, Juan P. H.
       Arhancet, Juan P.
       Delaney, Kimberly
       Hall, Kathleen B.
       Summers, Neena
       Oates, Edward
 
<120>  Compositions and Methods Using Capsids Resistant to Hydrolases

<130>  PCT APSE

<150>  US 61/836,833
<151>  2013-06-19

<150>  US 61/838,736
<151>  2013-06-24

<150>  US 61/857,965
<151>  2013-07-24

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  3569
<212>  RNA
<213>  Bacteriophage MS2

<400>  1
gggugggacc ccuuucgggg uccugcucaa cuuccugucg agcuaaugcc auuuuuaaug       60

ucuuuagcga gacgcuacca uggcuaucgc uguagguagc cggaauucca uuccuaggag      120

guuugaccug ugcgagcuuu uaguacccuu gauagggaga acgagaccuu cguccccucc      180

guucgcguuu acgcggacgg ugagacugaa gauaacucau ucucuuuaaa auaucguucg      240

aacuggacuc ccggucguuu uaacucgacu ggggccaaaa cgaaacagug gcacuacccc      300

ucuccguauu cacggggggc guuaaguguc acaucgauag aucaaggugc cuacaagcga      360

agugggucau cguggggucg cccguacgag gagaaagccg guuucggcuu cucccucgac      420

gcacgcuccu gcuacagccu cuucccugua agccaaaacu ugacuuacau cgaagugccg      480

cagaacguug cgaaccgggc gucgaccgaa guccugcaaa aggucaccca ggguaauuuu      540

aaccuuggug uugcuuuagc agaggccagg ucgacagccu cacaacucgc gacgcaaacc      600

auugcgcucg ugaaggcgua cacugccgcu cgucgcggua auuggcgcca ggcgcuccgc      660

uaccuugccc uaaacgaaga ucgaaaguuu cgaucaaaac acguggccgg caggugguug      720

gaguugcagu ucgguugguu accacuaaug agugauaucc agggugcaua ugagaugcuu      780

acgaagguuc accuucaaga guuucuuccu augagagccg uacgucaggu cgguacuaac      840

aucaaguuag auggccgucu gucguaucca gcugcaaacu uccagacaac gugcaacaua      900

ucgcgacgua ucgugauaug guuuuacaua aacgaugcac guuuggcaug guugucgucu      960

cuagguaucu ugaacccacu agguauagug ugggaaaagg ugccuuucuc auucguuguc     1020

gacuggcucc uaccuguagg uaacaugcuc gagggccuua cggcccccgu gggaugcucc     1080

uacaugucag gaacaguuac ugacguaaua acgggugagu ccaucauaag cguugacgcu     1140

cccuacgggu ggacugugga gagacagggc acugcuaagg cccaaaucuc agccaugcau     1200

cgagggguac aauccguaug gccaacaacu ggcgcguacg uaaagucucc uuucucgaug     1260

guccauaccu uagaugcguu agcauuaauc aggcaacggc ucucuagaua gagcccucaa     1320

ccggaguuug aagcauggcu ucuaacuuua cucaguucgu ucucgucgac aauggcggaa     1380

cuggcgacgu gacugucgcc ccaagcaacu ucgcuaacgg ggucgcugaa uggaucagcu     1440

cuaacucgcg uucacaggcu uacaaaguaa ccuguagcgu ucgucagagc ucugcgcaga     1500

aucgcaaaua caccaucaaa gucgaggugc cuaaaguggc aacccagacu guugguggug     1560

uagagcuucc uguagccgca uggcguucgu acuuaaauau ggaacuaacc auuccaauuu     1620

ucgcuacgaa uuccgacugc gagcuuauug uuaaggcaau gcaaggucuc cuaaaagaug     1680

gaaacccgau ucccucagca aucgcagcaa acuccggcau cuacuaauag acgccggcca     1740

uucaaacaug aggauuaccc augucgaaga caacaaagaa guucaacucu uuauguauug     1800

aucuuccucg cgaucuuucu cucgaaauuu accaaucaau ugcuucuguc gcuacuggaa     1860

gcggugaucc gcacagugac gacuuuacag caauugcuua cuuaagggac gaauugcuca     1920

caaagcaucc gaccuuaggu ucugguaaug acgaggcgac ccgucguacc uuagcuaucg     1980

cuaagcuacg ggaggcgaau ggugaucgcg gucagauaaa uagagaaggu uucuuacaug     2040

acaaauccuu gucaugggau ccggauguuu uacaaaccag cauccguagc cuuauuggca     2100

accuccucuc uggcuaccga ucgucguugu uugggcaaug cacguucucc aacggugcuc     2160

cuauggggca caaguugcag gaugcagcgc cuuacaagaa guucgcugaa caagcaaccg     2220

uuaccccccg cgcucugaga gcggcucuau ugguccgaga ccaaugugcg ccguggauca     2280

gacacgcggu ccgcuauaac gagucauaug aauuuaggcu cguuguaggg aacggagugu     2340

uuacaguucc gaagaauaau aaaauagauc gggcugccug uaaggagccu gauaugaaua     2400

uguaccucca gaaagggguc ggugcuuuca ucagacgccg gcucaaaucc guugguauag     2460

accugaauga ucaaucgauc aaccagcguc uggcucagca gggcagcgua gaugguucgc     2520

uugcgacgau agacuuaucg ucugcauccg auuccaucuc cgaucgccug guguggaguu     2580

uucucccacc agagcuauau ucauaucucg aucguauccg cucacacuac ggaaucguag     2640

auggcgagac gauacgaugg gaacuauuuu ccacaauggg aaauggguuc acauuugagc     2700

uagaguccau gauauucugg gcaauaguca aagcgaccca aauccauuuu gguaacgccg     2760

gaaccauagg caucuacggg gacgauauua uaugucccag ugagauugca ccccgugugc     2820

uagaggcacu ugccuacuac gguuuuaaac cgaaucuucg uaaaacguuc guguccgggc     2880

ucuuucgcga gagcugcggc gcgcacuuuu accguggugu cgaugucaaa ccguuuuaca     2940

ucaagaaacc uguugacaau cucuucgccc ugaugcugau auuaaaucgg cuacgggguu     3000

ggggaguugu cggagguaug ucagauccac gccucuauaa ggugugggua cggcucuccu     3060

cccaggugcc uucgauguuc uucgguggga cggaccucgc ugccgacuac uacguaguca     3120

gcccgccuac ggcagucucg guauacacca agacuccgua cgggcggcug cucgcggaua     3180

cccguaccuc ggguuuccgu cuugcucgua ucgcucgaga acgcaaguuc uucagcgaaa     3240

agcacgacag uggucgcuac auagcguggu uccauacugg aggugaaauc accgacagca     3300

ugaaguccgc cggcgugcgc guuauacgca cuucggagug gcuaacgccg guucccacau     3360

ucccucagga gugugggcca gcgagcucuc cucgguagcu gaccgaggga cccccguaaa     3420

cggggugggu gugcucgaaa gagcacgggu gcgaaagcgg uccggcucca ccgaaaggug     3480

ggcgggcuuc ggcccaggga ccucccccua aagagaggac ccgggauucu cccgauuugg     3540

uaacuagcug cuuggcuagu uaccaccca                                       3569


<210>  2
<211>  398
<212>  DNA
<213>  Bacteriophage MS2

<400>  2
artcaatggc ttctaacttt actcagttcg ttctcgtcga caatggcgga actggcgacg       60

tgactgtcgc cccaagcaac ttcgctaacg gggtcgctga atggatcagc tctaactcgc      120

gttcacaggc ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag aatcgcaaat      180

acaccatcaa agtcgaggtg cctaaagtgg caacccagac tgttggtggt gtagagcttc      240

ctgtagccgc atggcgttcg tacttaaata tggaactaac cattccaatt ttcgctacga      300

attccgactg cgagcttatt gttaaggcaa tgcaaggtct cctaaaagat ggaaacccga      360

ttccctcagc aatcgcagca aactccggca tctactaa                              398


<210>  3
<211>  130
<212>  PRT
<213>  Bacteriophage MS2

<400>  3

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Asn Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr 
    130 


<210>  4
<211>  480
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bacteriophage MS2 coat protein and pac sequence cloning cassette


<220>
<221>  gene
<222>  (42)..(435)

<220>
<221>  stem_loop
<222>  (454)..(472)

<400>  4
acaagtttgt acaaaaaagc aggctaagaa ggagatatac atatggcttc taactttact       60

cagttcgttc tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc      120

gctaacgggg tcgctgaatg gatcagctct aactcgcgtt cacaggctta caaagtaacc      180

tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct      240

aaagtggcaa cccagactgt tggtggtgta gagcttcctg tagccgcatg gcgttcgtac      300

ttaaatatgg aactaaccat tccaattttc gctacgaatt ccgactgcga gcttattgtt      360

aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcagcaat cgcagcaaac      420

tccggcatct actaatagac gccggccatt caaacatgag gattacccat gtacccagct      480


<210>  5
<211>  165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7-Rz2


<220>
<221>  promoter
<222>  (1)..(18)
<223>  T7

<220>
<221>  stem_loop
<222>  (19)..(67)
<223>  shRNA

<220>
<221>  misc_RNA
<222>  (68)..(130)
<223>  HDV ribozyme

<220>
<221>  misc_feature
<222>  (131)..(140)
<223>  AT spacer

<220>
<221>  stem_loop
<222>  (141)..(159)
<223>  MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (160)..(165)
<223>  NcoI restriction site

<400>  5
taatacgact cactataggc ttgtgatgct tcagccaaat caagagtttg gctgaagcat       60

cacaagcggc cggcatggtc ccagcctcct cgctggcgcc ggctgggcaa cattcgtggc      120

gaatgggacc atatatatat acatgaggat tacccatgtc catgg                      165


<210>  6
<211>  221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7-Rz3


<220>
<221>  promoter
<222>  (1)..(18)
<223>  T7

<220>
<221>  misc_RNA
<222>  (19)..(74)
<223>  Hammerhead ribozyme

<220>
<221>  stem_loop
<222>  (75)..(123)
<223>  shRNA

<220>
<221>  misc_RNA
<222>  (124)..(186)
<223>  HDV ribozyme

<220>
<221>  misc_feature
<222>  (187)..(196)
<223>  AT spacer

<220>
<221>  stem_loop
<222>  (197)..(215)
<223>  MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (216)..(221)
<223>  NcoI restriction site

<400>  6
taatacgact cactataggg gagagcacac aagcctgatg agtccgtgag gacgaaacgg       60

tacccggtac cgtcgcttgt gatgcttcag ccaaatcaag agtttggctg aagcatcaca      120

agcggccggc atggtcccag cctcctcgct ggcgccggct gggcaacatt cgtggcgaat      180

gggaccatat atatatacat gaggattacc catgtccatg g                          221


<210>  7
<211>  179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7-Rz6


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriciton site

<220>
<221>  promoter
<222>  (7)..(24)
<223>  T7

<220>
<221>  stem_loop
<222>  (25)..(74)
<223>  shRNA

<220>
<221>  misc_RNA
<222>  (75)..(137)
<223>  HDV ribozyme

<220>
<221>  stem_loop
<222>  (147)..(165)
<223>  MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (174)..(179)
<223>  NcoI restriciton site

<400>  7
ggatcctaat acgactcact ataggcaagc tgaccctgaa gttctcaaga ggaacttcag       60

ggtcagcttg ccaaggccgg catggtccca gcctcctcgc tggcgccggc tgggcaacat      120

tcgtggcgaa tgggaccacg cttcaaacat gaggattacc catgtcgaag cgaccatgg       179


<210>  8
<211>  362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7-Rz8


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(24)
<223>  T7

<220>
<221>  misc_RNA
<222>  (25)..(84)
<223>  Hammerhead ribozyme

<220>
<221>  misc_feature
<222>  (85)..(105)
<223>  sense strand siRNA

<220>
<221>  misc_RNA
<222>  (106)..(168)
<223>  HDV ribozyme

<220>
<221>  misc_feature
<222>  (169)..(172)
<223>  AT spacer

<220>
<221>  misc_RNA
<222>  (173)..(239)
<223>  Hammerhead ribozyme

<220>
<221>  misc_feature
<222>  (240)..(262)
<223>  antisense strand siRNA

<220>
<221>  misc_RNA
<222>  (263)..(325)
<223>  HDV ribozyme

<220>
<221>  stem_loop
<222>  (263)..(325)
<223>  MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (263)..(325)
<223>  NcoI restriction site

<400>  8
ggatcctaat acgactcact atagggagaa tgaacttcag ggtcagcttg ctgatgaggc       60

gcttcggcgc cgaaacaccg tgtccaagct gaccctgaag ttcatggccg gcatggtccc      120

agcctcctcg ctggcgccgg ctgggcaaca ttcgtggcga atgggaccat tagccaagct      180

gaccctgaag ttcatctgat gagactccga attcggagtc gaaacacggt aaccgtgtca      240

tgaacttcag ggtcagcttg gcggccggca tggtcccagc ctcctcgctg gcgccggctg      300

ggcaacattc gtggcgaatg ggacccattc aaacatgagg attacccatg tcgaagccat      360

gg                                                                     362


<210>  9
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7-Rz1


<220>
<221>  promoter
<222>  (1)..(18)
<223>  T7

<220>
<221>  misc_RNA
<222>  (19)..(64)
<223>  Hammerhead ribozyme

<220>
<221>  misc_RNA
<222>  (65)..(113)
<223>  siRNA

<220>
<221>  misc_RNA
<222>  (114)..(162)
<223>  Hammerhead ribozyme

<220>
<221>  misc_feature
<222>  (163)..(168)
<223>  NcoI restriction site

<400>  9
taatacgact cactataggc tcgagcaagc ctgatgaggc gcttcggcgc cgaaacaccg       60

tgtcgcttgt gatgcttcag ccaaatcaag agtttggctg aagcatcaca agctcaccgg      120

atgtgctctc cggtctgatg agtccgtgag gacgaaagct tgccatgg                   168


<210>  10
<211>  251
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7-Rz4


<220>
<221>  promoter
<222>  (1)..(18)
<223>  T7

<220>
<221>  misc_RNA
<222>  (19)..(98)
<223>  Hammerhead ribozyme

<220>
<221>  misc_RNA
<222>  (99)..(146)
<223>  siRNA

<220>
<221>  misc_RNA
<222>  (147)..(245)
<223>  Hammerhead ribozyme

<220>
<221>  misc_feature
<222>  (246)..(251)
<223>  NcoI restriction site

<400>  10
taatacgact cactataggg agaacgccgg ccattcaaat agtaaataat agagggtcag       60

cttgctgatg aggcgcttcg gcgccgaaac accgtgtcca agctgaccct gaagttcatc      120

aagagtgaac ttcagggtca gcttgtcacc ggatgtgctc tccggtctga tgagtccgtg      180

aggacgaaac aagctgaccc tgaagttcac tacgccggcc attcaaacat gaggattacc      240

catgtccatg g                                                           251


<210>  11
<211>  468
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  long sense strand RNA


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(26)
<223>  T7

<220>
<221>  misc_RNA
<222>  (27)..(318)
<223>  long sense strand RNA

<220>
<221>  stem_loop
<222>  (319)..(337)
<223>  MS2 specific packing sequence

<220>
<221>  terminator
<222>  (338)..(468)
<223>  Transcription terminator

<400>  11
ggatcctaat acgactcact atagggagat gggtggtgat cttggacaga tctacaggcg       60

attggtcaca gctgtgaacg agattgagaa gagggttcca ttttctcacg acgacaggct      120

tggatttctc accttctgtc caaccaatct tggaaccact gtccgggcat cagtccatat      180

taaggtgccc aagctggctg ccaacaaggc aaaacttgag gaagttgctg cacgatacaa      240

ccttcaggtc cgtggtactc gaggagaaca cacagaagct gagggaggag tctatgacat      300

ctcaaacaag aggaggaaac atgaggatca cccatgtgcg gccgcgctgc caccgctgag      360

caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa      420

cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaat                   468


<210>  12
<211>  467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  long antisense strand RNA


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(26)
<223>  T7

<220>
<221>  misc_RNA
<222>  (27)..(317)
<223>  long antisense RNA

<220>
<221>  stem_loop
<222>  (318)..(336)
<223>  MS2 specific packing sequence

<220>
<221>  terminator
<222>  (337)..(467)
<223>  Transcription terminator

<400>  12
ggatcctaat acgactcact atagggtcct cctcttgttt gagatgtcat agactcctcc       60

ctcagcttct gtgtgttctc ctcgagtacc acggacctga aggttgtatc gtgcagcaac      120

ttcctcaagt tttgccttgt tggcagccag cttgggcacc ttaatatgga ctgatgcccg      180

gacagtggtt ccaagattgg ttggacagaa ggtgagaaat ccaagcctgt cgtcgtgaga      240

aaatggaacc ctcttctcaa tctcgttcac agctgtgacc aatcgcctgt agatctgtcc      300

aagatcacca cccatctaca tgaggatcac ccatgtgcgg ccgcgctgcc accgctgagc      360

aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaac      420

ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaat                    467


<210>  13
<211>  319
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  long bulged RNA


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(26)
<223>  T7

<220>
<221>  stem_loop
<222>  (27)..(45)
<223>  MS2 specific packing sequence

<220>
<221>  misc_RNA
<222>  (46)..(146)
<223>  antisense strand long bulged RNA

<220>
<221>  misc_RNA
<222>  (147)..(155)
<223>  loop region of long bulged RNA

<220>
<221>  misc_RNA
<222>  (156)..(256)
<223>  sense strand long bulged RNA

<220>
<221>  misc_feature
<222>  (179)..(181)
<223>  bulge

<220>
<221>  misc_feature
<222>  (205)..(207)
<223>  bulge

<220>
<221>  misc_feature
<222>  (231)..(233)
<223>  bulge

<220>
<221>  misc_RNA
<222>  (257)..(319)
<223>  HDV ribozyme

<400>  13
ggatcctaat acgactcact atagggacat gaggatcacc catgtggaca gatctacagg       60

cgataatacc tgagattgag aagagggttc aatacctcga cgacaggctt ggatttaata      120

cctgggcatc agtccatatt aaaatacctg acccatattt taatatggac tgatgccccc      180

atattaaatc caagcctgtc gtcgccatat tgaaccctct tctcaatctc ccatattatc      240

gcctgtagat ctgtccggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac      300

attcgtggcg aatgggacc                                                   319


<210>  14
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A. tristis shRNA (single)


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriciton site

<220>
<221>  promoter
<222>  (7)..(26)
<223>  T7

<220>
<221>  stem_loop
<222>  (27)..(45)
<223>  MS2 specific packing sequence

<220>
<221>  misc_RNA
<222>  (46)..(66)
<223>  sense strand siRNA

<220>
<221>  misc_RNA
<222>  (67)..(73)
<223>  loop of siRNA

<220>
<221>  misc_RNA
<222>  (74)..(94)
<223>  antisense strand siRNA

<220>
<221>  misc_RNA
<222>  (95)..(157)
<223>  HDV ribozyme

<400>  14
ggatcctaat acgactcact atagggacat gaggatcacc catgtaaggg catcagtcca       60

tattaatcaa gagttaatat ggactgatgc ccttggccgg catggtccca gcctcctcgc      120

tggcgccggc tgggcaacat tcgtggcgaa tgggacc                               157


<210>  15
<211>  233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A. tristis shRNA (multiple)


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(26)
<223>  T7

<220>
<221>  stem_loop
<222>  (27)..(45)
<223>  MS2 specific pac

<220>
<221>  misc_feature
<222>  (46)..(54)
<223>  TAA spacer

<220>
<221>  misc_RNA
<222>  (55)..(75)
<223>  sense strand first siRNA

<220>
<221>  misc_RNA
<222>  (76)..(82)
<223>  loop of first siRNA

<220>
<221>  misc_RNA
<222>  (83)..(105)
<223>  antisense strand first siRNA

<220>
<221>  misc_feature
<222>  (106)..(112)
<223>  TAA spacer

<220>
<221>  misc_RNA
<222>  (113)..(133)
<223>  sense strand second siRNA

<220>
<221>  misc_RNA
<222>  (134)..(140)
<223>  loop of second siRNA

<220>
<221>  misc_RNA
<222>  (141)..(163)
<223>  antisense strand second siRNA

<220>
<221>  misc_feature
<222>  (164)..(170)
<223>  TA spacer

<220>
<221>  misc_RNA
<222>  (171)..(233)
<223>  HDV ribozyme

<400>  15
ggatcctaat acgactcact atagggacat gaggatcacc catgtaataa taataagggc       60

atcagtccat attaatcaag agttaatatg gactgatgcc cttcataata ataagaagtt      120

gctgcacgat acatcaagag tgtatcgtgc agcaacttct tcataataat ggccggcatg      180

gtcccagcct cctcgctggc gccggctggg caacattcgt ggcgaatggg acc             233


<210>  16
<211>  176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  half looped shRNA


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(26)
<223>  T7

<220>
<221>  stem_loop
<222>  (27)..(45)
<223>  MS2 specific packing sequence

<220>
<221>  misc_RNA
<222>  (46)..(66)
<223>  sense strand siRNA

<220>
<221>  misc_RNA
<222>  (67)..(69)
<223>  first half loop

<220>
<221>  stem_loop
<222>  (70)..(87)
<223>  second MS2 specific packing sequence

<220>
<221>  misc_RNA
<222>  (88)..(92)
<223>  second half loop

<220>
<221>  misc_RNA
<222>  (93)..(113)
<223>  antisense strand siRNA

<220>
<221>  misc_RNA
<222>  (114)..(176)
<223>  HDV ribozyme

<400>  16
ggatcctaat acgactcact atagggacat gaggatcacc catgtaaggg catcagtcca       60

tattaatcaa catgaggatc acccatgtag agttaatatg gactgatgcc cttggccggc      120

atggtcccag cctcctcgct ggcgccggct gggcaacatt cgtggcgaat gggacc          176


<210>  17
<211>  982
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  bacterial expression construct (E. coli)


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(25)
<223>  T7

<220>
<221>  stem_loop
<222>  (33)..(52)
<223>  first MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (53)..(74)
<223>  first MS2 sequence spacer

<220>
<221>  gene
<222>  (75)..(794)
<223>  EGFP gene

<220>
<221>  misc_feature
<222>  (795)..(809)
<223>  second MS2 sequence spacer

<220>
<221>  stem_loop
<222>  (810)..(828)
<223>  second MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (829)..(843)
<223>  third MS2 sequence spacer

<220>
<221>  misc_RNA
<222>  (844)..(906)
<223>  HDV ribozyme

<220>
<221>  misc_feature
<222>  (907)..(914)
<223>  NotI restriction site

<220>
<221>  terminator
<222>  (915)..(982)
<223>  Transcription terminator

<400>  17
ggatcctaat acgactcact ataggccatt cacacatgag gatcacccat gtggaagaca       60

acggagtttg aagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc      120

tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg      180

gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg      240

tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc      300

ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg      360

agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg      420

agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca      480

acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg      540

acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca      600

gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc      660

tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc      720

gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg      780

agctgtacaa gtagacgccg gccattcaga catgaggatc acccatgtcg aagacaacaa      840

agaggccggc atggtcccag cctcctcgct ggcgccggct gggcaacatt cgtggcgaat      900

gggaccgcgg ccgcgctgcc accgctgagc aataactagc ataacccctt ggggcctcta      960

aacgggtctt gaggggtttt tt                                               982


<210>  18
<211>  1398
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dicotyledenous plant expression construct


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(18)
<223>  T7

<220>
<221>  misc_feature
<222>  (19)..(33)
<223>  first MS2 sequence spacer

<220>
<221>  stem_loop
<222>  (34)..(53)
<223>  first MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (54)..(63)
<223>  second MS2 sequence spacer

<220>
<221>  misc_feature
<222>  (64)..(139)
<223>  first Carnation Italian ringspot virus sequence

<220>
<221>  gene
<222>  (140)..(859)
<223>  EGFP gene

<220>
<221>  misc_feature
<222>  (860)..(1210)
<223>  second Carnation Italian ringspot virus sequence

<220>
<221>  misc_feature
<222>  (1211)..(1225)
<223>  third MS2 sequence spacer

<220>
<221>  stem_loop
<222>  (1226)..(1244)
<223>  second MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (1245)..(1259)
<223>  fourth MS2 sequence spacer

<220>
<221>  misc_RNA
<222>  (1260)..(1322)
<223>  HDV ribozyme

<220>
<221>  misc_feature
<222>  (1323)..(1330)
<223>  NotI restriction site

<220>
<221>  terminator
<222>  (1331)..(1398)
<223>  Transcription terminator

<400>  18
ggatcctaat acgactcact ataggccatt cacacatgag gatcacccat gtggaagaca       60

acagaaatat ctcaggattt gaccgtccgg tgagttgcgc ttatcacttg cgtaaggtat      120

ttctctcttc attgttcata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc      180

catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg      240

cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct      300

gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg      360

ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt      420

ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa      480

gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga      540

cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat      600

ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga      660

cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt      720

gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga      780

gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat      840

ggacgagctg tacaagtagg ttcgctgaaa cgagtgtaaa tctggcatag catacaggtt      900

actcttgttg ggttctagat gttatgatga cgagtcggtt cgggctccgc actaggtttg      960

gtcacctagg ggatggagat atggaaaggg tctcgtgtgc tgttagacgg tcgtaagacg     1020

cgcttgcaac atgggcctat gaccggataa gtcatagcaa tactagccaa catgaatagg     1080

attcctgttt acgaaagtta gatgtcactt gtggaaacgg acccagacac ggttgatctc     1140

accttcgggg gggctataga gatcgctgga agcactaccg gacaaccgga acattgcaga     1200

aatgcagccc acgccggcca ttcagacatg aggatcaccc atgtcgaaga caacaaagag     1260

gccggcatgg tcccagcctc ctcgctggcg ccggctgggc aacattcgtg gcgaatggga     1320

ccgcggccgc gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg     1380

ggtcttgagg ggtttttt                                                   1398


<210>  19
<211>  1246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  monocotyledenous plant expression construct


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(24)
<223>  T7

<220>
<221>  misc_feature
<222>  (25)..(33)
<223>  first MS2 sequence spacer

<220>
<221>  stem_loop
<222>  (34)..(52)
<223>  first MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (53)..(62)
<223>  second MS2 sequence spacer

<220>
<221>  misc_feature
<222>  (63)..(139)
<223>  first Carnation Italian ringspot virus sequence

<220>
<221>  gene
<222>  (140)..(859)
<223>  EGFP coding sequence

<220>
<221>  misc_feature
<222>  (860)..(1058)
<223>  second Carnation Italian ringspot virus sequence

<220>
<221>  misc_feature
<222>  (959)..(971)
<223>  Maize necrotic streak virus sequence

<220>
<221>  misc_feature
<222>  (1059)..(1073)
<223>  third MS2 sequence spacer

<220>
<221>  stem_loop
<222>  (1074)..(1092)
<223>  second MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (1093)..(1107)
<223>  fourth MS2 sequence spacer

<220>
<221>  misc_RNA
<222>  (1108)..(1170)
<223>  HDV ribozyme

<220>
<221>  misc_feature
<222>  (1171)..(1178)
<223>  NotI restriction site

<220>
<221>  terminator
<222>  (1179)..(1246)
<223>  Transcription termnator

<400>  19
ggatcctaat acgactcact ataggccatt cacacatgag gatcacccat gtggaagaca       60

acagaaatat ctcaggattt gaccgtccgg tgagttgcgc ttatcacttg cgtaaggtat      120

ttctctcttc attgttcata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc      180

catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg      240

cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct      300

gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg      360

ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt      420

ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa      480

gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga      540

cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat      600

ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga      660

cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt      720

gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga      780

gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat      840

ggacgagctg tacaagtagt gtctgatgat gtgaggaacg tggactgtga tgtggtggtg      900

cggtaccatg gctggtcacc atggtaatgc gtagggcaac acagttcatt aagactcact      960

gatgatggca ccagacacgg ttgatctcac cttcgggggg gctatagaga tcgctggaag     1020

cactaccgga caaccggaac attgcagaaa tgcagcccac gccggccatt cagacatgag     1080

gatcacccat gtcgaagaca acaaagaggc cggcatggtc ccagcctcct cgctggcgcc     1140

ggctgggcaa cattcgtggc gaatgggacc gcggccgcgc tgccaccgct gagcaataac     1200

tagcataacc ccttggggcc tctaaacggg tcttgagggg tttttt                    1246


<210>  20
<211>  1774
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mammalian expression construct


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  BamHI restriction site

<220>
<221>  promoter
<222>  (7)..(24)
<223>  T7

<220>
<221>  misc_feature
<222>  (25)..(33)
<223>  first MS2 sequence spacer

<220>
<221>  stem_loop
<222>  (34)..(52)
<223>  first MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (53)..(62)
<223>  second MS2 sequence spacer

<220>
<221>  misc_feature
<222>  (63)..(561)
<223>  Encephalomyocarditis virus IRES sequence

<220>
<221>  gene
<222>  (562)..(1343)
<223>  EGFP gene

<220>
<221>  polyA_signal
<222>  (1344)..(1586)
<223>  SV40 late poly(A) signal sequence

<220>
<221>  misc_feature
<222>  (1587)..(1601)
<223>  third MS2 sequence spacer

<220>
<221>  stem_loop
<222>  (1602)..(1620)
<223>  second MS2 specific packing sequence

<220>
<221>  misc_feature
<222>  (1621)..(1635)
<223>  fourth MS2 sequence spacer

<220>
<221>  misc_RNA
<222>  (1636)..(1698)
<223>  HDV ribozyme

<220>
<221>  misc_feature
<222>  (1699)..(1706)
<223>  NotI restriction site

<220>
<221>  terminator
<222>  (1707)..(1774)
<223>  Transcription terminator

<400>  20
ggatcctaat acgactcact ataggccatt cacacatgag gatcacccat gtggaagaca       60

accccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct      120

atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc      180

ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc      240

tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg      300

tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa      360

agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt      420

ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg      480

atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta      540

catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt      600

cctttgaaaa acacgatgat aatatggtga gcaagggcga ggagctgttc accggggtgg      660

tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg      720

agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca      780

agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca      840

gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct      900

acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg      960

tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg     1020

aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata     1080

tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg     1140

aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc     1200

ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca     1260

acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg     1320

gcatggacga gctgtacaag tagttctaga gcggccgctt cgagcagaca tgataagata     1380

cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga     1440

aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa     1500

caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag     1560

caagtaaaac ctctacaaat gtggtaacgc cggccattca gacatgagga tcacccatgt     1620

cgaagacaac aaagaggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca     1680

ttcgtggcga atgggaccgc ggccgcgctg ccaccgctga gcaataacta gcataacccc     1740

ttggggcctc taaacgggtc ttgaggggtt tttt                                 1774


