                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  PRODUCTION OF POLYPEPTIDES WITHOUT SECRETION SIGNAL IN BACILLUS

<130>  12592-WO-PCT

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  500
<212>  PRT
<213>  Thermus thermophilus

<400>  1

Met Glu Leu Pro Arg Ala Phe Gly Leu Leu Leu His Pro Thr Ser Leu 
1               5                   10                  15      


Pro Gly Pro Tyr Gly Val Gly Val Leu Gly Gln Glu Ala Arg Asp Phe 
            20                  25                  30          


Leu Arg Phe Leu Lys Glu Ala Gly Gly Arg Tyr Trp Gln Val Leu Pro 
        35                  40                  45              


Leu Gly Pro Thr Gly Tyr Gly Asp Ser Pro Tyr Gln Ser Phe Ser Ala 
    50                  55                  60                  


Phe Ala Gly Asn Pro Tyr Leu Ile Asp Leu Arg Pro Leu Ala Glu Arg 
65                  70                  75                  80  


Gly Tyr Val Arg Leu Glu Asp Pro Gly Phe Pro Gln Gly Arg Val Asp 
                85                  90                  95      


Tyr Gly Leu Leu Tyr Ala Trp Lys Trp Pro Ala Leu Lys Glu Ala Phe 
            100                 105                 110         


Arg Gly Phe Lys Glu Lys Ala Ser Pro Glu Glu Arg Glu Ala Phe Ala 
        115                 120                 125             


Ala Phe Arg Glu Arg Glu Ala Trp Trp Leu Glu Asp Tyr Ala Leu Phe 
    130                 135                 140                 


Met Ala Leu Lys Gly Ala His Gly Gly Leu Pro Trp Asn Arg Trp Pro 
145                 150                 155                 160 


Leu Pro Leu Arg Lys Arg Glu Glu Lys Ala Leu Arg Glu Ala Lys Ser 
                165                 170                 175     


Ala Leu Ala Glu Glu Val Ala Phe His Ala Phe Thr Gln Trp Leu Phe 
            180                 185                 190         


Phe Arg Gln Trp Gly Ala Leu Lys Ala Glu Ala Glu Ala Leu Gly Ile 
        195                 200                 205             


Arg Ile Ile Gly Asp Met Pro Ile Phe Val Ala Glu Asp Ser Ala Glu 
    210                 215                 220                 


Val Trp Ala His Pro Glu Trp Phe His Leu Asp Glu Glu Gly Arg Pro 
225                 230                 235                 240 


Thr Val Val Ala Gly Val Pro Pro Asp Tyr Phe Ser Glu Thr Gly Gln 
                245                 250                 255     


Arg Trp Gly Asn Pro Leu Tyr Arg Trp Asp Val Leu Glu Arg Glu Gly 
            260                 265                 270         


Phe Ser Phe Trp Ile Arg Arg Leu Glu Lys Ala Leu Glu Leu Phe His 
        275                 280                 285             


Leu Val Arg Ile Asp His Phe Arg Gly Phe Glu Ala Tyr Trp Glu Ile 
    290                 295                 300                 


Pro Ala Ser Cys Pro Thr Ala Val Glu Gly Arg Trp Val Lys Ala Pro 
305                 310                 315                 320 


Gly Glu Lys Leu Phe Gln Lys Ile Gln Glu Val Phe Gly Glu Val Pro 
                325                 330                 335     


Val Leu Ala Glu Asp Leu Gly Val Ile Thr Pro Glu Val Glu Ala Leu 
            340                 345                 350         


Arg Asp Arg Phe Gly Leu Pro Gly Met Lys Val Leu Gln Phe Ala Phe 
        355                 360                 365             


Asp Asp Gly Met Glu Asn Pro Phe Leu Pro His Asn Tyr Pro Ala His 
    370                 375                 380                 


Gly Arg Val Val Val Tyr Thr Gly Thr His Asp Asn Asp Thr Thr Leu 
385                 390                 395                 400 


Gly Trp Tyr Arg Thr Ala Thr Pro His Glu Lys Ala Phe Met Ala Arg 
                405                 410                 415     


Tyr Leu Ala Asp Trp Gly Ile Thr Phe Arg Glu Glu Glu Glu Val Pro 
            420                 425                 430         


Trp Ala Leu Met His Leu Gly Met Lys Ser Val Ala Arg Leu Ala Val 
        435                 440                 445             


Tyr Pro Val Gln Asp Val Leu Ala Leu Gly Ser Glu Ala Arg Met Asn 
    450                 455                 460                 


Tyr Pro Gly Arg Pro Ser Gly Asn Trp Ala Trp Arg Leu Leu Pro Gly 
465                 470                 475                 480 


Glu Leu Ser Pro Glu His Gly Ala Arg Leu Arg Ala Met Ala Glu Ala 
                485                 490                 495     


Thr Glu Arg Leu 
            500 


<210>  2
<211>  238
<212>  PRT
<213>  artificial sequence

<220>
<223>  An F64L S65T amino acid substitution variant of GFP from the 
       marine jellyfish Aequorea Victoria.

<400>  2

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly Gln Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Tyr Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Met Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Pro Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Lys Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Ile Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  3
<211>  326
<212>  PRT
<213>  Pyrococcus furiosus

<400>  3

Met Lys Ile Leu Leu Ile Gly Met Gly Gly Thr Ile Ala Ser Val Lys 
1               5                   10                  15      


Gly Glu Asn Gly Tyr Glu Ala Ser Leu Ser Val Lys Glu Val Leu Asp 
            20                  25                  30          


Ile Ala Gly Ile Lys Asp Cys Glu Asp Cys Asp Phe Leu Asp Leu Lys 
        35                  40                  45              


Asn Val Asp Ser Thr Leu Ile Gln Pro Glu Asp Trp Val Asp Leu Ala 
    50                  55                  60                  


Glu Thr Leu Tyr Lys Asn Val Lys Lys Tyr Asp Gly Ile Ile Val Thr 
65                  70                  75                  80  


His Gly Thr Asp Thr Leu Ala Tyr Thr Ser Ser Met Ile Ser Phe Met 
                85                  90                  95      


Leu Arg Asn Pro Pro Ile Pro Ile Val Phe Thr Gly Ser Met Ile Pro 
            100                 105                 110         


Ala Thr Glu Glu Asn Ser Asp Ala Pro Leu Asn Leu Gln Thr Ala Ile 
        115                 120                 125             


Lys Phe Ala Thr Ser Gly Ile Arg Gly Val Tyr Val Ala Phe Asn Gly 
    130                 135                 140                 


Lys Val Met Leu Gly Val Arg Thr Ser Lys Val Arg Thr Met Ser Arg 
145                 150                 155                 160 


Asp Ala Phe Glu Ser Ile Asn Tyr Pro Ile Ile Ala Glu Leu Arg Gly 
                165                 170                 175     


Glu Asp Leu Val Val Asn Phe Ile Pro Lys Phe Asn Asn Gly Glu Val 
            180                 185                 190         


Thr Leu Asp Leu Arg His Asp Pro Lys Val Leu Val Ile Lys Leu Ile 
        195                 200                 205             


Pro Gly Leu Ser Gly Asp Ile Phe Arg Ala Ala Val Glu Leu Gly Tyr 
    210                 215                 220                 


Arg Gly Ile Val Ile Glu Gly Tyr Gly Ala Gly Gly Ile Pro Tyr Arg 
225                 230                 235                 240 


Gly Ser Asp Leu Leu Gln Thr Ile Glu Glu Leu Ser Lys Glu Ile Pro 
                245                 250                 255     


Ile Val Met Thr Thr Gln Ala Met Tyr Asp Gly Val Asp Leu Thr Arg 
            260                 265                 270         


Tyr Lys Val Gly Arg Leu Ala Leu Arg Ala Gly Val Ile Pro Ala Gly 
        275                 280                 285             


Asp Met Thr Lys Glu Ala Thr Val Thr Lys Leu Met Trp Ile Leu Gly 
    290                 295                 300                 


His Thr Asn Asn Val Glu Glu Ile Lys Val Leu Met Arg Lys Asn Leu 
305                 310                 315                 320 


Val Gly Glu Leu Arg Asp 
                325     


