                               SEQUENCE LISTING

<110> THE UNIVERSITY OF HONG KONG
 
<120> METHODS OF USING 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE TO 
      ENHANCE GROWTH AND/OR SEED YIELD OF GENETICALLY MODIFIED PLANTS

<130> 56720-130938

<140>
<141>

<150> 61/836,739
<151> 2013-06-19

<160> 34    

<170> PatentIn version 3.5

<210> 1
<211> 1686
<212> DNA
<213> Brassica juncea

<400> 1
gaccgaacct tcgatctcat tctctggaga agaaagttca atcctttttg attcaacaat       60

ggcgaagaac gtagggatat tggctatgga catctatttc cctcccacct gtgttcaaca      120

ggaagctttg gaagctcatg atggagcaag caaagggaaa tacaccattg gacttggtca      180

agattgttta gctttttgca ctgagcttga agatgttatc tcaatgagtt tcaatgcggt      240

gacatctctt cttgaaaagt acaagattga ccccaagcaa attgggcgtc ttgaagtagg      300

cagtgagact gtcattgaca agagcaagtc catcaaaacc ttcttgatgc aactctttga      360

gaaatgtgga aacactgatg tagaaggtgt tgactcgacc aatgcttgtt atggtggaac      420

tgcggctttg ctaaactgtg tcaattgggt tgagagtaac tcctgggatg gacgctatgg      480

ccttgtcatt tgtactgaca gtgcggttta tgcagaaggg cccgcaaggc ccactggagg      540

agcagctgcc attgctatgt tgataggacc agatgctcct attgtttttg agagcaaatt      600

gagaggaagc cacatggctc atgtctatga cttttacaag ccaaatcttg ctagcgagta      660

cccggttgtt gatggtaagc tttcacagac atgctacctt atggcacttg actcttgcta      720

taaacattta tgcaacaagt ttgagaaact tgagggcaaa gagttctcca tcaatgatgc      780

tgattacttt gtcttccatt ctccatacaa caaacttgtg cagaaaagct ttgctcgtct      840

cttgtacaac gacttcttga gaaacgcaag ctccattgat gaggctgcca aagaaaagtt      900

cactccttat tcctccttgt ccctcgatga gagttaccaa agccgtgatc ttgaaaaggt      960

gtcacaacaa ctagcaaaaa cgtattatga tgctaaagtg caaccaacga ctttagtacc     1020

aaaacaagtc ggtaacatgt acactgcttc tctctacgca gcatttgctt ccctcgtcca     1080

caacaaacac agcgatttgg cgggaaagcg ggtggttatg ttctcatatg gaagtggttc     1140

aaccgcaaca atgttctcat tgcgtctctg cgagaaccag tctcctttca gcttatcaaa     1200

catcgcttct gtaatggatg ttggcggtaa attgaaggct agacatgagt atgcacctga     1260

gaagtttgtg gagacaatga agctaatgga acataggtac ggagcaaagg agtttgtgac     1320

gagcaaggaa ggcatcttag accttttggc tccggggact tattatctga aagaggttga     1380

ttcattgtac cggagattct atggcaagaa aggagacgat ggatccataa ccaatggaca     1440

ctgattcttg aaagcataaa tcacttgaaa gattgtgtct actcattgct ctttatgttt     1500

actttccaaa attctagacg tttttataag gttcttgtta ctttgattta gcgacagttt     1560

gcatgctctt gtttttattt atttgccatt gattctctga agcttttttt ttttctctta     1620

gtgtgtgttt taaataagcc cgttaatttt aggaattatt aagatgttat aataataaac     1680

ctttaa                                                                1686


<210> 2
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      35S promoter forward primer

<400> 2
caatcccact atccttcgca agacc                                             25


<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      BjHMGS1 3'-end cDNA reverse ML264 primer

<400> 3
ggatccataa ccaatggaca ctgaggatcc                                        30


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      ML915 primer

<400> 4
cattgctatg ttgataggac                                                   20


<210> 5
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 5
Asp Glu Ser Tyr Gln Ser Arg Asp Leu Glu Lys Val Ser Gln Gln 
1               5                   10                  15  


<210> 6
<211> 461
<212> PRT
<213> Brassica juncea

<400> 6
Met Ala Lys Asn Val Gly Ile Leu Ala Met Asp Ile Tyr Phe Pro Pro 
1               5                   10                  15      


Thr Cys Val Gln Gln Glu Ala Leu Glu Ala His Asp Gly Ala Ser Lys 
            20                  25                  30          


Gly Lys Tyr Thr Ile Gly Leu Gly Gln Asp Cys Leu Ala Phe Cys Thr 
        35                  40                  45              


Glu Leu Glu Asp Val Ile Ser Met Ser Phe Asn Ala Val Thr Ser Leu 
    50                  55                  60                  


Leu Glu Lys Tyr Lys Ile Asp Pro Lys Gln Ile Gly Arg Leu Glu Val 
65                  70                  75                  80  


Gly Ser Glu Thr Val Ile Asp Lys Ser Lys Ser Ile Lys Thr Phe Leu 
                85                  90                  95      


Met Gln Leu Phe Glu Lys Cys Gly Asn Thr Asp Val Glu Gly Val Asp 
            100                 105                 110         


Ser Thr Asn Ala Cys Tyr Gly Gly Thr Ala Ala Leu Leu Asn Cys Val 
        115                 120                 125             


Asn Trp Val Glu Ser Asn Ser Trp Asp Gly Arg Tyr Gly Leu Val Ile 
    130                 135                 140                 


Cys Thr Asp Ser Ala Val Tyr Ala Glu Gly Pro Ala Arg Pro Thr Gly 
145                 150                 155                 160 


Gly Ala Ala Ala Ile Ala Met Leu Ile Gly Pro Asp Ala Pro Ile Val 
                165                 170                 175     


Phe Glu Ser Lys Leu Arg Gly Ser His Met Ala His Val Tyr Asp Phe 
            180                 185                 190         


Tyr Lys Pro Asn Leu Ala Ser Glu Tyr Pro Val Val Asp Gly Lys Leu 
        195                 200                 205             


Ser Gln Thr Cys Tyr Leu Met Ala Leu Asp Ser Cys Tyr Lys His Leu 
    210                 215                 220                 


Cys Asn Lys Phe Glu Lys Leu Glu Gly Lys Glu Phe Ser Ile Asn Asp 
225                 230                 235                 240 


Ala Asp Tyr Phe Val Phe His Ser Pro Tyr Asn Lys Leu Val Gln Lys 
                245                 250                 255     


Ser Phe Ala Arg Leu Leu Tyr Asn Asp Phe Leu Arg Asn Ala Ser Ser 
            260                 265                 270         


Ile Asp Glu Ala Ala Lys Glu Lys Phe Thr Pro Tyr Ser Ser Leu Ser 
        275                 280                 285             


Leu Asp Glu Ser Tyr Gln Ser Arg Asp Leu Glu Lys Val Ser Gln Gln 
    290                 295                 300                 


Leu Ala Lys Thr Tyr Tyr Asp Ala Lys Val Gln Pro Thr Thr Leu Val 
305                 310                 315                 320 


Pro Lys Gln Val Gly Asn Met Tyr Thr Ala Ser Leu Tyr Ala Ala Phe 
                325                 330                 335     


Ala Ser Leu Val His Asn Lys His Ser Asp Leu Ala Gly Lys Arg Val 
            340                 345                 350         


Val Met Phe Ser Tyr Gly Ser Gly Ser Thr Ala Thr Met Phe Ser Leu 
        355                 360                 365             


Arg Leu Cys Glu Asn Gln Ser Pro Phe Ser Leu Ser Asn Ile Ala Ser 
    370                 375                 380                 


Val Met Asp Val Gly Gly Lys Leu Lys Ala Arg His Glu Tyr Ala Pro 
385                 390                 395                 400 


Glu Lys Phe Val Glu Thr Met Lys Leu Met Glu His Arg Tyr Gly Ala 
                405                 410                 415     


Lys Glu Phe Val Thr Ser Lys Glu Gly Ile Leu Asp Leu Leu Ala Pro 
            420                 425                 430         


Gly Thr Tyr Tyr Leu Lys Glu Val Asp Ser Leu Tyr Arg Arg Phe Tyr 
        435                 440                 445             


Gly Lys Lys Gly Asp Asp Gly Ser Ile Thr Asn Gly His 
    450                 455                 460     


<210> 7
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      ML276 primer

<400> 7
ggatccatgg cgaagaacgt agggatattg                                        30


<210> 8
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      ML860 primer

<400> 8
ggagactggt tctcgcagag ac                                                22


<210> 9
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      ML1046 primer

<400> 9
ccataattac accagcagtg tcc                                               23


<210> 10
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      ML1047 primer

<400> 10
caactgtgcc aacctcaata gaag                                              24


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtHMGR1-specific ML1879 primer

<400> 11
ttggcatcgg atttgttcag                                                   20


<210> 12
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtHMGR1-specific ML 1880 primer

<400> 12
ggcggctatc ttcctcaat                                                    19


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtHMGR2-specific ML1881 primer

<400> 13
agcaggtggc gtgagaaaat                                                   20


<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtHMGR2-specific ML1882 primer

<400> 14
cgaacgactg aacaaatccg                                                   20


<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtIPI1-specific ML1885 primer

<400> 15
atcgggtttg ttcagtcgtt c                                                 21


<210> 16
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtIPI1-specific ML1886 primer

<400> 16
gcaggtccac gacgactatc t                                                 21


<210> 17
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtIPI2-specific ML1887 primer

<400> 17
attgaggaga atgctcttgg tg                                                22


<210> 18
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtIPI2-specific ML1888 primer

<400> 18
ctggtcaact gggacatctt ca                                                22


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtFPPS-specific ML1889 primer

<400> 19
cttctccgca accacatcac                                                   20


<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtFPPS-specific ML1890 primer

<400> 20
gaggcagtct ggaactcaac c                                                 21


<210> 21
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSQS-specific ML1678 primer

<400> 21
aggaggtgga aacaactgat ga                                                22


<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSQS-specific ML1679 primer

<400> 22
agaacatacg gcacttgggt                                                   20


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtGGPPS1-specific ML1891 primer

<400> 23
gaccaatcca acgctatgcc                                                   20


<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtGGPPS1-specific ML1892 primer

<400> 24
ttatggttcg tcggctttcc                                                   20


<210> 25
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSMT1-2-specific ML1893 primer

<400> 25
tccaaataac gaagagcacg aa                                                22


<210> 26
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSMT1-2-specific ML1894 primer

<400> 26
caggcgaatc ctcagcaag                                                    19


<210> 27
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSMT2-1-specific ML1895 primer

<400> 27
aaaccgccga taaagtccc                                                    19


<210> 28
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSMT2-1-specific ML1896 primer

<400> 28
tctacagcca tttcttcgtg aa                                                22


<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSMT2-2-specific ML1897 primer

<400> 29
atccacggta ttgaaagggg                                                   20


<210> 30
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtSMT2-2-specific ML1898 primer

<400> 30
tttcagcctc gtccaccac                                                    19


<210> 31
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtCYP85A1-specific ML1899 primer

<400> 31
aagaaaagcc ttcctcctgg ta                                                22


<210> 32
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtCYP85A1-specific ML1900 primer

<400> 32
aaaactccca aatctggctc tt                                                22


<210> 33
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtACTIN-specific ML1951 primer

<400> 33
tcacagaagc tcctcctaat cca                                               23


<210> 34
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      NtACTIN-specific ML1952 primer

<400> 34
gagggaaaga acagcctgaa tg                                                22


