                         LISTA DE SECUENCIAS

<110>  Consejo Superior de Investigaciones Cientficas
       Universitat de Valencia
 
<120>  Secuencia de nucletidos codificante de una enzima con actividad dextransacarasa,
       clulas que la expresan y su uso para la obtencin de exopolisacridos con actividad
       antiviral y composiciones que los contienen

<130>  2013-0330

<140>  P201330831
<141>  2013-06-05

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1345
<212>  DNA
<213>  Lactobacillus sakei MN1

<220>
<223>  Secuencia del DNA codificante de la regin variable del 16S RNA
       de Lactobacillus sakei MN1

<400>  1
gctgatccgc gattactagc gattccggct tcatgtaggc gagttgcagc ctacaatccg       60

aactgagaat ggttttaaga gattagctaa acctcgcggt ctcgcaactc gttgtaccat      120

ccattgtagc acgtgtgtag cccaggtcat aaggggcatg atgatttgac gtcgtcccca      180

ccttcctccg gtttgtcacc ggcagtctca ctagagtgcc caactaaatg ctggcaacta      240

gtaataaggg ttgcgctcgt tgcgggactt aacccaacat ctcacgacac gagctgacga      300

caaccatgca ccacctgtca ctttgtcccc gaagggaaag ctctatctct agagtggtca      360

aaggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca catgctccac      420

cgcttgtgcg ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg      480

cggagtgctt aatgcgttag ctgcggcact gaagggcgga aaccctccaa cacctagcac      540

tcatcgttta cggcatggac taccagggta tctaatcctg tttgctaccc atgctttcga      600

gcctcagcgt cagttacaga ccagacagcc gccttcgcca ctggtgttct tccatatatc      660

tacgcatttc accgctacac atggagttcc actgtcctct tctgcactca agtttcccag      720

tttccgatgc acttcttcgg ttgagccgaa ggctttcaca tcagacttaa gaaaccgcct      780

gcgctcgctt tacgcccaat aaatccggac aacgcttgcc acctacgtat taccgcggct      840

gctggcacgt agttagccgt ggctttctgg ttggataccg tcactacctg atcagttact      900

atcagataca ttcttctcca acaacagagt tttacgatcc gaaaaccttc ttcactcacg      960

cggcgttgct ccatcagact ttcgtccatt gtggaagatt ccctactgct gcctcccgta     1020

ggagtctggg ccgtgtctca gtcccagtgt ggccgattac cctctcaggt cggctatgca     1080

tcacggtctt ggtgagcctt tacctcacca actaactaat gcaccgcggg tccatcctaa     1140

agtgatagcc gaaaccatct ttcaacccta caccatgcgg tgttaggttt tatgcggtat     1200

tagcatctgt ttccaaatgt tatcccccac tttagggcag gttacccacg tgttactcac     1260

ccgtccgcca ctcactcaaa tgtttatcaa tcaggagcaa gctccttcaa tctaaacgag     1320

agtgcgttcg acttgcatgt atagc                                           1345


<210>  2
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cebador sentido para la amplificacin de una regin del gen dsrLS
       de Lactobacillus sakei MN1

<400>  2
gatgatggtc aatatatggc aa                                                22


<210>  3
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cebador antisentido para la amplificacin de una regin del gen 
       dsrLS de Lactobacillus sakei MN1

<400>  3
cttgaacgat attgtggtgc caa                                               23


<210>  4
<211>  5304
<212>  DNA
<213>  Lactobacillus sakei MN1

<220>
<223>  Secuencia de nucletidos del gen dsrLS de Lactobacillus sakei MN1

<400>  4
atgttaagga ataattattt tggagagact aaaacgcatt ataaattata taaatgcggt       60

aagaactggg ctgtcatggg gatttcatta tttccgctgg gattagggat gctagttacc      120

agccagccag tgtcagctga tgtgacagcc accagcacct caagcagtgc agtgaggacc      180

gatgcaatca gtgaaagtag tagcagtgca gcaaaggctg aaacgactag tgcaagtagt      240

agcagtgcag tgaaggccga aacgactagt gcaagtagta gcagtgcagc aaaggctgaa      300

acggctgcga tcactactgc aggtgttgca aatgctgatt cacaaacatc agcagaagta      360

accgctgact ctacttctac cagccaagtg gtaactaata attccaataa tcaaaataat      420

acagcacagc cagccggtca agaagcagcc ccggtatcag aggacacatc atctgatgat      480

agtgagagaa ctacaccaac agttgcaaat aatgataagc cagcaattga ttcagttgac      540

acttcacaac ctgcaactgc agcgccaaaa gcagacactg atgtatcaac gctacaagta      600

gatgcaacta cgaagaccga ttcagacata aaagaggata caccaacaga taagacaacc      660

gatacaaaga ctgtgcaatt aaccactgtt gaaggaacgt ccaagcaagt ggtaacgacg      720

ccgaaggaag agagctcaac tgacaaatct tcgtctgtgg tttctaaaca gacagacaaa      780

acgtctttgc caaccgtagc aacagcaaca gcgacgacag tgtctaagat tccttcagtg      840

acaggtgatt accagtttga cgaaaagacg aagacttata cgttcacagg taaagatggt      900

catcccgtaa ctgggttggt ttacgcgaat aatatcctgc aatactttga tgaaacgggt      960

catcaagtaa aaggtcaata cgttacaatt gcaggtcatg tatattattt cgacccagcc     1020

agcggcgctg cacaaacagg tgttaatcaa atcgatggta agatggttgg gtttaaatct     1080

gatgggtcac aaattacgtc aggtttttct aatgataacg ccggaaattc ttactacttt     1140

gatgagtctg gaaccatggt gacagggcgg caaactattg ctggtaagac gtattacttt     1200

gacaaagacg ggcatctccg taaggggtat tccactatta ttgataatca attgtactat     1260

ttcgatttga aaacaggaga gtctgtttca acaacgacgt ccaatttcaa atctggcttg     1320

acatcacaaa cggatgacac aacaccacat aatagtgcgg ttaatatgtc taaggatagt     1380

tttaccaccg ttgatggatt cttgacagct gagtcatggt atgtacctaa agatattcaa     1440

acatctgcga cggactggcg tgcatcaacg cctgaagatt tccgtccgat catgatgact     1500

tggtggccaa cgaagcaaat tcaagcagcg tatttgaacc atatggtctc cgaaggattg     1560

ttgtcatcag ataagaagtt ctccgcaacg gatgatcaaa cgttgttgaa ccaagcagca     1620

cacgcggttc aattgcaaat tgaattgaag attcaacaga caaagtctgt tgaatggttg     1680

cgaacaacga tgcacaattt cattaagtca caaccaggat acaatgttac tagtgaaacg     1740

ccaagtaacg accaccttca aggtggcgca ttaagctaca ttaacagtgt tttgacgcct     1800

gatgcgaact caaatttccg tttgatgaac cgtaatccaa cacaacaaga tggtacgcgt     1860

cattacaaca ctgatacatc tgagggtgga tatgagttgc tgttagccaa tgacgtggat     1920

aattctaacc cagttgttca agcagaacaa ttgaactggt tgtacttctt gacgcatttc     1980

ggtgaaattg ttaagaacga tccgtcagct aactttgata gtgttagagt ggatgcggta     2040

gacaacgtgg atgccgacct gctaaacatt acagccgctt attttagaga tgtgtatggc     2100

gtcgataaaa acgatttgac agctaatcaa catttgtcta ttttggaaga ttggggccac     2160

aatgacccat tatatgtcaa ggaccacggt agtgatcagt tgacgatgga tgattacatg     2220

catacccaat tgatttggtc attaacaaaa aatccagata atcgtagtgc gatgcgacga     2280

tttatggagt attatttggt cgaccgtgct aaggacaata cgtctgatca ggcaattcct     2340

aattacagct ttgtccgtgc acacgatagt gaagttcaaa cggttatcgg tgatattgtt     2400

gcgaagttgt atccggatgt taaaaattca ttggcgccat ctatggaaca attggcggca     2460

gcctttaagg tatatgatgc ggatatgaat tctgttaata agaagtatac gcaatacaac     2520

atgccagcag cgtatgccat gttactaacg aataaagaca caattccacg tgtttactat     2580

ggtgatatgt atacggatga tggtcaatat atggcaacta agtcaccata ttacgatgcc     2640

atctcagcgt tgttgaaagc ccgtattaag tatgtggctg gtggacaaac catggctgta     2700

gataaacacg atatcttaac atcagttcgc tttggtgatg ggatcatgaa tgcatctgat     2760

aagggtagca cgacggcccg tacccaagga attggcgtga ttgtcagcaa taatgatgcg     2820

ttagcgttga agggagacac tgtgaccctt catatgggta tcgctcacgc caaccaggca     2880

taccgtgctt tgttgttaac gacgacagat ggactaatga aatacacgtc cgataatggc     2940

gcgccaattc gctatacgga tgcaaatggt gacttgattt tcactagcgc agacattaag     3000

ggataccaaa acgttgaggt atccggattc ttgtcagtgt gggtaccagt cggtgcatcc     3060

gacacacagg atgcgcgcgc aacagggtct agcgctgcaa acaaaactgg tgacacctta     3120

cattcaaatg cagcattgga ctcaaatgtg atttatgaag gtttttctaa tttccaagag     3180

atgccaacaa cccacgatga gtttacaaac gtaaagatcg ctcaaaatgc tgatttgttt     3240

aagtcatggg gtgtgacaag tttccaactt gcaccacaat atcgttcaag tgatgacaca     3300

tcatttttgg attctattat taagaatgga tatgcgttta cagaccgcta tgacttgggc     3360

tttaatacgc caacaaagta cggagacgtt gacgacctag cagatgcaat tagagcaatg     3420

cacagtgttg gtattcaggt catggctgac tttgtccctg accaaattta taatttgcca     3480

ggtcaagaag tagttgctgt taatcgtact aataactttg gtacaccaaa ccaggattca     3540

gatctacaaa accagttgta tgttacaaat tcaaagggtg gcggtgaata ccaagctaag     3600

tatggtggtg agttcttgga tcttttgcgt ctggaacacc ctgatttgtt tacaacaaat     3660

cagatttcga ctggtgtacc aatcgatggg tccacgaaga ttaaagaatg gtctgcaaag     3720

tacttcaatg gttctgacat ccaaggtaag ggcgctgatt acgtattgaa ggatggtgca     3780

tctcaagaat atttcaagat tacgtctaat gcgaacgatg agtccttctt gccaaaacaa     3840

tttatgaatc aagatgccat gactgggttc accacagatg aaaagggcac aacttattat     3900

tcaacaagtg gttaccaagc taaacagtcg tttatccaag gtgatgatgg acaatattat     3960

tactttgatg cagacggata catggtgacg ggctcacaaa ccattaatgg taagcaatat     4020

tacttcttgc caaatggcgt tgagttaaga gaagcatttt tacaaaatgc atctggtaac     4080

acggtttatt atggcaagac tggctcagca gttaagtcta aatatgtagt cgatcaaagc     4140

ggtgtggctt attactttga tgtaaacggt aatatggtcg cagatcgtat gatgattctt     4200

gatggacaca cgcaatattt ctttgcgggt ggttcacaag ctaaggacca atttttgatt     4260

gggtcagatg gtaacttacg ttactttgac caaggtagtg gtaatatggt tacaaatcgt     4320

tttgcagtaa accgaaacgg ggattggttc tacttcaatg gcgatggtat cgcgttgaag     4380

ggttggcaaa caattgctgg taagacttat ttctttgatg ctgatggacg tcaagtcaag     4440

gctgccgctg acaaggctgc tgctgacaag gctgccgctg aacaagccgc tgctgacaag     4500

gccgctgctg acaaggctgc cgctgaacaa gctgccgctg acaaggctgc tgctgacaag     4560

gctgccgctg aacaagctgc cgctgacaag gctgccgctg acaaggctgc cgctgaacaa     4620

gctgccgctg acaaggctgc tgctgacaag gctgccgctg aacaagctgc cgctgaacaa     4680

gctgccgctg acaaggctgc cgctgaacaa gccgctgctg acaaggctgc cgctgaacaa     4740

gccgctactg acaaggccgc tgctgacaag gctgccgctg aacaagctgc cgctgacaag     4800

gctgctgctg acaaggctgc cgctgaacaa gctgccgctg aacaagctgc cgctgacaag     4860

gctgccgctg aacaagctgc tgctgacaag gctgccgctg aacaagctgc cgctgacaag     4920

gctgccgctg aacaagccgc tgctgacaag gctgccgcta aggataagca aactcaagct     4980

gtagcttacg ctgctaccaa ggctaagaac aatattgatc aagctactac agctgatggc     5040

atcaatgatg cccaagcaac tggtatcact gatattgata accagcatgt tcctggtact     5100

tctgttgata atcaaaagca agctgagaag gtaactgaag atatcaagaa tgatccagat     5160

aataagactt tgcctgaagc tatcgaatta ccaaatacgg gcgttgataa gacagaaagt     5220

attactatta ccggtgtagt tatgctaatc ctcactacta tttttggtct gttgtttaca     5280

agtaaaaagc ataaaaaaga ttag                                            5304


<210>  5
<211>  1767
<212>  PRT
<213>  Lactobacillus sakei MN1

<220>
<223>  Secuencia de aminocidos de la protena DsrLS de 
       Lactobacillus sakei MN1

<400>  5

Met Leu Arg Asn Asn Tyr Phe Gly Glu Thr Lys Thr His Tyr Lys Leu 
1               5                   10                  15      


Tyr Lys Cys Gly Lys Asn Trp Ala Val Met Gly Ile Ser Leu Phe Pro 
            20                  25                  30          


Leu Gly Leu Gly Met Leu Val Thr Ser Gln Pro Val Ser Ala Asp Val 
        35                  40                  45              


Thr Ala Thr Ser Thr Ser Ser Ser Ala Val Arg Thr Asp Ala Ile Ser 
    50                  55                  60                  


Glu Ser Ser Ser Ser Ala Ala Lys Ala Glu Thr Thr Ser Ala Ser Ser 
65                  70                  75                  80  


Ser Ser Ala Val Lys Ala Glu Thr Thr Ser Ala Ser Ser Ser Ser Ala 
                85                  90                  95      


Ala Lys Ala Glu Thr Ala Ala Ile Thr Thr Ala Gly Val Ala Asn Ala 
            100                 105                 110         


Asp Ser Gln Thr Ser Ala Glu Val Thr Ala Asp Ser Thr Ser Thr Ser 
        115                 120                 125             


Gln Val Val Thr Asn Asn Ser Asn Asn Gln Asn Asn Thr Ala Gln Pro 
    130                 135                 140                 


Ala Gly Gln Glu Ala Ala Pro Val Ser Glu Asp Thr Ser Ser Asp Asp 
145                 150                 155                 160 


Ser Glu Arg Thr Thr Pro Thr Val Ala Asn Asn Asp Lys Pro Ala Ile 
                165                 170                 175     


Asp Ser Val Asp Thr Ser Gln Pro Ala Thr Ala Ala Pro Lys Ala Asp 
            180                 185                 190         


Thr Asp Val Ser Thr Leu Gln Val Asp Ala Thr Thr Lys Thr Asp Ser 
        195                 200                 205             


Asp Ile Lys Glu Asp Thr Pro Thr Asp Lys Thr Thr Asp Thr Lys Thr 
    210                 215                 220                 


Val Gln Leu Thr Thr Val Glu Gly Thr Ser Lys Gln Val Val Thr Thr 
225                 230                 235                 240 


Pro Lys Glu Glu Ser Ser Thr Asp Lys Ser Ser Ser Val Val Ser Lys 
                245                 250                 255     


Gln Thr Asp Lys Thr Ser Leu Pro Thr Val Ala Thr Ala Thr Ala Thr 
            260                 265                 270         


Thr Val Ser Lys Ile Pro Ser Val Thr Gly Asp Tyr Gln Phe Asp Glu 
        275                 280                 285             


Lys Thr Lys Thr Tyr Thr Phe Thr Gly Lys Asp Gly His Pro Val Thr 
    290                 295                 300                 


Gly Leu Val Tyr Ala Asn Asn Ile Leu Gln Tyr Phe Asp Glu Thr Gly 
305                 310                 315                 320 


His Gln Val Lys Gly Gln Tyr Val Thr Ile Ala Gly His Val Tyr Tyr 
                325                 330                 335     


Phe Asp Pro Ala Ser Gly Ala Ala Gln Thr Gly Val Asn Gln Ile Asp 
            340                 345                 350         


Gly Lys Met Val Gly Phe Lys Ser Asp Gly Ser Gln Ile Thr Ser Gly 
        355                 360                 365             


Phe Ser Asn Asp Asn Ala Gly Asn Ser Tyr Tyr Phe Asp Glu Ser Gly 
    370                 375                 380                 


Thr Met Val Thr Gly Arg Gln Thr Ile Ala Gly Lys Thr Tyr Tyr Phe 
385                 390                 395                 400 


Asp Lys Asp Gly His Leu Arg Lys Gly Tyr Ser Thr Ile Ile Asp Asn 
                405                 410                 415     


Gln Leu Tyr Tyr Phe Asp Leu Lys Thr Gly Glu Ser Val Ser Thr Thr 
            420                 425                 430         


Thr Ser Asn Phe Lys Ser Gly Leu Thr Ser Gln Thr Asp Asp Thr Thr 
        435                 440                 445             


Pro His Asn Ser Ala Val Asn Met Ser Lys Asp Ser Phe Thr Thr Val 
    450                 455                 460                 


Asp Gly Phe Leu Thr Ala Glu Ser Trp Tyr Val Pro Lys Asp Ile Gln 
465                 470                 475                 480 


Thr Ser Ala Thr Asp Trp Arg Ala Ser Thr Pro Glu Asp Phe Arg Pro 
                485                 490                 495     


Ile Met Met Thr Trp Trp Pro Thr Lys Gln Ile Gln Ala Ala Tyr Leu 
            500                 505                 510         


Asn His Met Val Ser Glu Gly Leu Leu Ser Ser Asp Lys Lys Phe Ser 
        515                 520                 525             


Ala Thr Asp Asp Gln Thr Leu Leu Asn Gln Ala Ala His Ala Val Gln 
    530                 535                 540                 


Leu Gln Ile Glu Leu Lys Ile Gln Gln Thr Lys Ser Val Glu Trp Leu 
545                 550                 555                 560 


Arg Thr Thr Met His Asn Phe Ile Lys Ser Gln Pro Gly Tyr Asn Val 
                565                 570                 575     


Thr Ser Glu Thr Pro Ser Asn Asp His Leu Gln Gly Gly Ala Leu Ser 
            580                 585                 590         


Tyr Ile Asn Ser Val Leu Thr Pro Asp Ala Asn Ser Asn Phe Arg Leu 
        595                 600                 605             


Met Asn Arg Asn Pro Thr Gln Gln Asp Gly Thr Arg His Tyr Asn Thr 
    610                 615                 620                 


Asp Thr Ser Glu Gly Gly Tyr Glu Leu Leu Leu Ala Asn Asp Val Asp 
625                 630                 635                 640 


Asn Ser Asn Pro Val Val Gln Ala Glu Gln Leu Asn Trp Leu Tyr Phe 
                645                 650                 655     


Leu Thr His Phe Gly Glu Ile Val Lys Asn Asp Pro Ser Ala Asn Phe 
            660                 665                 670         


Asp Ser Val Arg Val Asp Ala Val Asp Asn Val Asp Ala Asp Leu Leu 
        675                 680                 685             


Asn Ile Thr Ala Ala Tyr Phe Arg Asp Val Tyr Gly Val Asp Lys Asn 
    690                 695                 700                 


Asp Leu Thr Ala Asn Gln His Leu Ser Ile Leu Glu Asp Trp Gly His 
705                 710                 715                 720 


Asn Asp Pro Leu Tyr Val Lys Asp His Gly Ser Asp Gln Leu Thr Met 
                725                 730                 735     


Asp Asp Tyr Met His Thr Gln Leu Ile Trp Ser Leu Thr Lys Asn Pro 
            740                 745                 750         


Asp Asn Arg Ser Ala Met Arg Arg Phe Met Glu Tyr Tyr Leu Val Asp 
        755                 760                 765             


Arg Ala Lys Asp Asn Thr Ser Asp Gln Ala Ile Pro Asn Tyr Ser Phe 
    770                 775                 780                 


Val Arg Ala His Asp Ser Glu Val Gln Thr Val Ile Gly Asp Ile Val 
785                 790                 795                 800 


Ala Lys Leu Tyr Pro Asp Val Lys Asn Ser Leu Ala Pro Ser Met Glu 
                805                 810                 815     


Gln Leu Ala Ala Ala Phe Lys Val Tyr Asp Ala Asp Met Asn Ser Val 
            820                 825                 830         


Asn Lys Lys Tyr Thr Gln Tyr Asn Met Pro Ala Ala Tyr Ala Met Leu 
        835                 840                 845             


Leu Thr Asn Lys Asp Thr Ile Pro Arg Val Tyr Tyr Gly Asp Met Tyr 
    850                 855                 860                 


Thr Asp Asp Gly Gln Tyr Met Ala Thr Lys Ser Pro Tyr Tyr Asp Ala 
865                 870                 875                 880 


Ile Ser Ala Leu Leu Lys Ala Arg Ile Lys Tyr Val Ala Gly Gly Gln 
                885                 890                 895     


Thr Met Ala Val Asp Lys His Asp Ile Leu Thr Ser Val Arg Phe Gly 
            900                 905                 910         


Asp Gly Ile Met Asn Ala Ser Asp Lys Gly Ser Thr Thr Ala Arg Thr 
        915                 920                 925             


Gln Gly Ile Gly Val Ile Val Ser Asn Asn Asp Ala Leu Ala Leu Lys 
    930                 935                 940                 


Gly Asp Thr Val Thr Leu His Met Gly Ile Ala His Ala Asn Gln Ala 
945                 950                 955                 960 


Tyr Arg Ala Leu Leu Leu Thr Thr Thr Asp Gly Leu Met Lys Tyr Thr 
                965                 970                 975     


Ser Asp Asn Gly Ala Pro Ile Arg Tyr Thr Asp Ala Asn Gly Asp Leu 
            980                 985                 990         


Ile Phe Thr Ser Ala Asp Ile Lys  Gly Tyr Gln Asn Val  Glu Val Ser 
        995                 1000                 1005             


Gly Phe  Leu Ser Val Trp Val  Pro Val Gly Ala Ser  Asp Thr Gln 
    1010                 1015                 1020             


Asp Ala  Arg Ala Thr Gly Ser  Ser Ala Ala Asn Lys  Thr Gly Asp 
    1025                 1030                 1035             


Thr Leu  His Ser Asn Ala Ala  Leu Asp Ser Asn Val  Ile Tyr Glu 
    1040                 1045                 1050             


Gly Phe  Ser Asn Phe Gln Glu  Met Pro Thr Thr His  Asp Glu Phe 
    1055                 1060                 1065             


Thr Asn  Val Lys Ile Ala Gln  Asn Ala Asp Leu Phe  Lys Ser Trp 
    1070                 1075                 1080             


Gly Val  Thr Ser Phe Gln Leu  Ala Pro Gln Tyr Arg  Ser Ser Asp 
    1085                 1090                 1095             


Asp Thr  Ser Phe Leu Asp Ser  Ile Ile Lys Asn Gly  Tyr Ala Phe 
    1100                 1105                 1110             


Thr Asp  Arg Tyr Asp Leu Gly  Phe Asn Thr Pro Thr  Lys Tyr Gly 
    1115                 1120                 1125             


Asp Val  Asp Asp Leu Ala Asp  Ala Ile Arg Ala Met  His Ser Val 
    1130                 1135                 1140             


Gly Ile  Gln Val Met Ala Asp  Phe Val Pro Asp Gln  Ile Tyr Asn 
    1145                 1150                 1155             


Leu Pro  Gly Gln Glu Val Val  Ala Val Asn Arg Thr  Asn Asn Phe 
    1160                 1165                 1170             


Gly Thr  Pro Asn Gln Asp Ser  Asp Leu Gln Asn Gln  Leu Tyr Val 
    1175                 1180                 1185             


Thr Asn  Ser Lys Gly Gly Gly  Glu Tyr Gln Ala Lys  Tyr Gly Gly 
    1190                 1195                 1200             


Glu Phe  Leu Asp Leu Leu Arg  Leu Glu His Pro Asp  Leu Phe Thr 
    1205                 1210                 1215             


Thr Asn  Gln Ile Ser Thr Gly  Val Pro Ile Asp Gly  Ser Thr Lys 
    1220                 1225                 1230             


Ile Lys  Glu Trp Ser Ala Lys  Tyr Phe Asn Gly Ser  Asp Ile Gln 
    1235                 1240                 1245             


Gly Lys  Gly Ala Asp Tyr Val  Leu Lys Asp Gly Ala  Ser Gln Glu 
    1250                 1255                 1260             


Tyr Phe  Lys Ile Thr Ser Asn  Ala Asn Asp Glu Ser  Phe Leu Pro 
    1265                 1270                 1275             


Lys Gln  Phe Met Asn Gln Asp  Ala Met Thr Gly Phe  Thr Thr Asp 
    1280                 1285                 1290             


Glu Lys  Gly Thr Thr Tyr Tyr  Ser Thr Ser Gly Tyr  Gln Ala Lys 
    1295                 1300                 1305             


Gln Ser  Phe Ile Gln Gly Asp  Asp Gly Gln Tyr Tyr  Tyr Phe Asp 
    1310                 1315                 1320             


Ala Asp  Gly Tyr Met Val Thr  Gly Ser Gln Thr Ile  Asn Gly Lys 
    1325                 1330                 1335             


Gln Tyr  Tyr Phe Leu Pro Asn  Gly Val Glu Leu Arg  Glu Ala Phe 
    1340                 1345                 1350             


Leu Gln  Asn Ala Ser Gly Asn  Thr Val Tyr Tyr Gly  Lys Thr Gly 
    1355                 1360                 1365             


Ser Ala  Val Lys Ser Lys Tyr  Val Val Asp Gln Ser  Gly Val Ala 
    1370                 1375                 1380             


Tyr Tyr  Phe Asp Val Asn Gly  Asn Met Val Ala Asp  Arg Met Met 
    1385                 1390                 1395             


Ile Leu  Asp Gly His Thr Gln  Tyr Phe Phe Ala Gly  Gly Ser Gln 
    1400                 1405                 1410             


Ala Lys  Asp Gln Phe Leu Ile  Gly Ser Asp Gly Asn  Leu Arg Tyr 
    1415                 1420                 1425             


Phe Asp  Gln Gly Ser Gly Asn  Met Val Thr Asn Arg  Phe Ala Val 
    1430                 1435                 1440             


Asn Arg  Asn Gly Asp Trp Phe  Tyr Phe Asn Gly Asp  Gly Ile Ala 
    1445                 1450                 1455             


Leu Lys  Gly Trp Gln Thr Ile  Ala Gly Lys Thr Tyr  Phe Phe Asp 
    1460                 1465                 1470             


Ala Asp  Gly Arg Gln Val Lys  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1475                 1480                 1485             


Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1490                 1495                 1500             


Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1505                 1510                 1515             


Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1520                 1525                 1530             


Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1535                 1540                 1545             


Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala Glu Gln  Ala Ala Ala 
    1550                 1555                 1560             


Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1565                 1570                 1575             


Glu Gln  Ala Ala Thr Asp Lys  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1580                 1585                 1590             


Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1595                 1600                 1605             


Glu Gln  Ala Ala Ala Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1610                 1615                 1620             


Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala 
    1625                 1630                 1635             


Asp Lys  Ala Ala Ala Glu Gln  Ala Ala Ala Asp Lys  Ala Ala Ala 
    1640                 1645                 1650             


Lys Asp  Lys Gln Thr Gln Ala  Val Ala Tyr Ala Ala  Thr Lys Ala 
    1655                 1660                 1665             


Lys Asn  Asn Ile Asp Gln Ala  Thr Thr Ala Asp Gly  Ile Asn Asp 
    1670                 1675                 1680             


Ala Gln  Ala Thr Gly Ile Thr  Asp Ile Asp Asn Gln  His Val Pro 
    1685                 1690                 1695             


Gly Thr  Ser Val Asp Asn Gln  Lys Gln Ala Glu Lys  Val Thr Glu 
    1700                 1705                 1710             


Asp Ile  Lys Asn Asp Pro Asp  Asn Lys Thr Leu Pro  Glu Ala Ile 
    1715                 1720                 1725             


Glu Leu  Pro Asn Thr Gly Val  Asp Lys Thr Glu Ser  Ile Thr Ile 
    1730                 1735                 1740             


Thr Gly  Val Val Met Leu Ile  Leu Thr Thr Ile Phe  Gly Leu Leu 
    1745                 1750                 1755             


Phe Thr  Ser Lys Lys His Lys  Lys Asp 
    1760                 1765         


<210>  6
<211>  1674
<212>  DNA
<213>  Lactobacillus sakei MN1

<220>
<223>  Secuencia de nucletidos del replicn del plsmido 
       pMN1 de Lactobacillus sakei MN1. Origen de replicacin: 
       1..>192. Gen repA: 237..>1172. Gen repB: 1165..>1674

<400>  6
cctcttttaa acctctttta taaacctctt ttaaacctct tttagacccc tcttgaggct       60

tactctccca aggcttatag aagtttatcg actacatttt gtctgtttat cgactacatt      120

ttgtctgttt atcgactaca ttttgtctgt ttatcgacta cattttgtct gtttatcgac      180

tacacttatt tacttctgta ttcaaataaa gtagtattat tcaaggaggt tattttatga      240

gcaatgaatt agttaaatat gatccagaac tgaatacaat tcctctgaga agatttactc      300

ctgtagaaat gaatcttttc ttttcggttg tttctagaat gcgcgataaa ggtgatgata      360

ctgttcggtt tacttttgat cagttaaaag aattaagcgc gtataagccg accgcaaata      420

atcgatttat tgatgacata caaagtacat atcaaaaaat attaggtctt agatttggct      480

ctagaagtaa agatggactt gatagagaaa tgtttgtcat gtttactcga tttgaaatca      540

agggttcggc agaagtccct tatgttgata ttcaaattta ccccaaggca ttgaaacttc      600

taaataatct tgaaagctgg gttcgatatg ctttagcaga gttcagagat ttaaagagta      660

gttatgcaaa aacgatgttt cgtctcctta aacaatttcg aactactggt tatgcttatt      720

tttctaaaag tgattttttt gaattacttg atattccgca aagctattgg aataaacctg      780

caaacgttga atccagagtt attcagccaa ttaaagaaga actaactcct cttttcaggg      840

gtctagctat tagaaaaaaa tatggtaaag gccgtggcaa gccggttatt ggttactcat      900

ttacctggaa atcagaaaaa aagaacgcag acgacttctc acaaggtcaa tttcaagatg      960

aacgtcaaaa actctttaac attcagcata atggtgaatt aacagagcag gaaaaatggc     1020

gtgccattga caaagttaag gggttgactt taggctccac tgagaaacaa gcattggctg     1080

agaaacaagc ggaacacgat aaaaaaataa gagatcaagc aagaaaagaa gcacttgctg     1140

aactccgaaa ggggtttgga aatcatgcct aaaactatta gagaacttgc tgatgaattg     1200

ggcgtttcaa agcaaagaat tcaacaaatt atcgccaaat tatcggcaag taaaacacca     1260

aataaggaag gcaatagata cgttttaaac gcccaagatg tcaaaaatat aaaggatttg     1320

atgggttttg ataatgacaa gtcatcgaca agtgaatcga caaatagact tgtcgattat     1380

gatgtttact tagatgtgat agattccata aaagaaaaag atgaacagat aaaaatttta     1440

ttagaagttc aaaaacaaac acaaaatctt ctagatcagc aacaacggtt agccttacag     1500

gacaaacaac tgctcgaaga atacaaggca gaaattaagg acttgaaagc cttaacgatg     1560

gcaccacatg atgatggtga aaaaaagatg gcagcacaaa aacaaccgga accagaacat     1620

agcactcctg attcgcaacc taaacctaaa aagtggtggc gttttggtaa atag           1674


<210>  7
<211>  311
<212>  PRT
<213>  Lactobacillus sakei MN1

<220>
<223>  Secuencia de aminocidos de la protena RepA del plsmido pMN1 de
       Lactobacillus sakei MN1

<400>  7

Met Ser Asn Glu Leu Val Lys Tyr Asp Pro Glu Leu Asn Thr Ile Pro 
1               5                   10                  15      


Leu Arg Arg Phe Thr Pro Val Glu Met Asn Leu Phe Phe Ser Val Val 
            20                  25                  30          


Ser Arg Met Arg Asp Lys Gly Asp Asp Thr Val Arg Phe Thr Phe Asp 
        35                  40                  45              


Gln Leu Lys Glu Leu Ser Ala Tyr Lys Pro Thr Ala Asn Asn Arg Phe 
    50                  55                  60                  


Ile Asp Asp Ile Gln Ser Thr Tyr Gln Lys Ile Leu Gly Leu Arg Phe 
65                  70                  75                  80  


Gly Ser Arg Ser Lys Asp Gly Leu Asp Arg Glu Met Phe Val Met Phe 
                85                  90                  95      


Thr Arg Phe Glu Ile Lys Gly Ser Ala Glu Val Pro Tyr Val Asp Ile 
            100                 105                 110         


Gln Ile Tyr Pro Lys Ala Leu Lys Leu Leu Asn Asn Leu Glu Ser Trp 
        115                 120                 125             


Val Arg Tyr Ala Leu Ala Glu Phe Arg Asp Leu Lys Ser Ser Tyr Ala 
    130                 135                 140                 


Lys Thr Met Phe Arg Leu Leu Lys Gln Phe Arg Thr Thr Gly Tyr Ala 
145                 150                 155                 160 


Tyr Phe Ser Lys Ser Asp Phe Phe Glu Leu Leu Asp Ile Pro Gln Ser 
                165                 170                 175     


Tyr Trp Asn Lys Pro Ala Asn Val Glu Ser Arg Val Ile Gln Pro Ile 
            180                 185                 190         


Lys Glu Glu Leu Thr Pro Leu Phe Arg Gly Leu Ala Ile Arg Lys Lys 
        195                 200                 205             


Tyr Gly Lys Gly Arg Gly Lys Pro Val Ile Gly Tyr Ser Phe Thr Trp 
    210                 215                 220                 


Lys Ser Glu Lys Lys Asn Ala Asp Asp Phe Ser Gln Gly Gln Phe Gln 
225                 230                 235                 240 


Asp Glu Arg Gln Lys Leu Phe Asn Ile Gln His Asn Gly Glu Leu Thr 
                245                 250                 255     


Glu Gln Glu Lys Trp Arg Ala Ile Asp Lys Val Lys Gly Leu Thr Leu 
            260                 265                 270         


Gly Ser Thr Glu Lys Gln Ala Leu Ala Glu Lys Gln Ala Glu His Asp 
        275                 280                 285             


Lys Lys Ile Arg Asp Gln Ala Arg Lys Glu Ala Leu Ala Glu Leu Arg 
    290                 295                 300                 


Lys Gly Phe Gly Asn His Ala 
305                 310     


<210>  8
<211>  169
<212>  PRT
<213>  Lactobacillus sakei MN1

<220>
<223>  Secuencia de aminocidos de la protena RepB del plsmido pMN1de 
       Lactobacillus sakei MN1

<400>  8

Met Pro Lys Thr Ile Arg Glu Leu Ala Asp Glu Leu Gly Val Ser Lys 
1               5                   10                  15      


Gln Arg Ile Gln Gln Ile Ile Ala Lys Leu Ser Ala Ser Lys Thr Pro 
            20                  25                  30          


Asn Lys Glu Gly Asn Arg Tyr Val Leu Asn Ala Gln Asp Val Lys Asn 
        35                  40                  45              


Ile Lys Asp Leu Met Gly Phe Asp Asn Asp Lys Ser Ser Thr Ser Glu 
    50                  55                  60                  


Ser Thr Asn Arg Leu Val Asp Tyr Asp Val Tyr Leu Asp Val Ile Asp 
65                  70                  75                  80  


Ser Ile Lys Glu Lys Asp Glu Gln Ile Lys Ile Leu Leu Glu Val Gln 
                85                  90                  95      


Lys Gln Thr Gln Asn Leu Leu Asp Gln Gln Gln Arg Leu Ala Leu Gln 
            100                 105                 110         


Asp Lys Gln Leu Leu Glu Glu Tyr Lys Ala Glu Ile Lys Asp Leu Lys 
        115                 120                 125             


Ala Leu Thr Met Ala Pro His Asp Asp Gly Glu Lys Lys Met Ala Ala 

    130                 135                 140                 


Gln Lys Gln Pro Glu Pro Glu His Ser Thr Pro Asp Ser Gln Pro Lys 
145                 150                 155                 160 


Pro Lys Lys Trp Trp Arg Phe Gly Lys 
                165                 


