                                  SEQUENCE LISTING
         
         <110>  Novo Nordisk A/S
          
         <120>  Method for making mature insulin polypeptides
         
         <130>  8694WO01
         
         <160>  14    
         
         <170>  PatentIn version 3.5
         
         <210>  1
         <211>  85
         <212>  PRT
         <213>  S. cerevisiae
         
         <400>  1
         
         Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
         1               5                   10                  15      
         
         
         Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
                     20                  25                  30          
         
         
         Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
                 35                  40                  45              
         
         
         Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
             50                  55                  60                  
         
         
         Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
         65                  70                  75                  80  
         
         
         Ser Met Ala Lys Arg 
                         85  
         
         
         <210>  2
         <211>  6
         <212>  PRT
         <213>  synthetic
         
         <400>  2
         
         Glu Glu Gly Glu Pro Lys 
         1               5       
         
         
         <210>  3
         <211>  39
         <212>  DNA
         <213>  synthetic
         
         <400>  3
         atacaggaat tccattcaag atctgttcaa acaagaaga                              39
         
         
         <210>  4
         <211>  106
         <212>  DNA
         <213>  synthetic
         
         
         <220>
         <221>  misc_feature
         <222>  (86)..(88)
         <223>  n is A, C, T or G and m is A or C
         
         <400>  4
         aatcttagtt tctagactag ttgcagtagt tttccaattg gtacaaggag cagatggagg       60
         
         tacagcattg ttcgacaata cccttmnnag ccttaggagt gtagaa                     106
         
         
         <210>  5
         <211>  432
         <212>  DNA
         <213>  synthetic
         
         <400>  5
         atgagatttc cttcaatttt tactgcagtt ttattcgcag catcctccgc attagctgct       60
         
         ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt      120
         
         tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat      180
         
         aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta      240
         
         tccatggcta agagagaaga aggtgaacca aagttcgtta accaacactt gtgcggttcc      300
         
         cacttggttg aagctttgta cttggtttgc ggtgaaagag gtttcttcta cactcctaag      360
         
         gctgctaagg gtattgtcga acaatgctgt acctccatct gctccttgta ccaattggaa      420
         
         aactactgca ac                                                          432
         
         
         <210>  6
         <211>  144
         <212>  PRT
         <213>  synthetic
         
         <400>  6
         
         Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
         1               5                   10                  15      
         
         
         Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
                     20                  25                  30          
         
         
         Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
                 35                  40                  45              
         
         
         Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
             50                  55                  60                  
         
         
         Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
         65                  70                  75                  80  
         
         
         Ser Met Ala Lys Arg Glu Glu Gly Glu Pro Lys Phe Val Asn Gln His 
                         85                  90                  95      
         
         
         Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu 
                     100                 105                 110         
         
         
         Arg Gly Phe Phe Tyr Thr Pro Lys Ala Ala Lys Gly Ile Val Glu Gln 
                 115                 120                 125             
         
         
         Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
             130                 135                 140                 
         
         
         <210>  7
         <211>  85
         <212>  PRT
         <213>  synthetic
         
         <400>  7
         
         Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
         1               5                   10                  15      
         
         
         Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
                     20                  25                  30          
         
         
         Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
                 35                  40                  45              
         
         
         Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
             50                  55                  60                  
         
         
         Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
         65                  70                  75                  80  
         
         
         Ser Met Ala Lys Arg 
                         85  
         
         
         <210>  8
         <211>  66
         <212>  PRT
         <213>  synthetic
         
         <400>  8
         
         Met Lys Leu Lys Thr Val Arg Ser Ala Val Leu Ser Ser Leu Phe Ala 
         1               5                   10                  15      
         
         
         Ser Gln Val Leu Gly Gln Pro Ile Asp Asp Thr Glu Ser Asn Thr Thr 
                     20                  25                  30          
         
         
         Ser Val Asn Leu Met Ala Asp Asp Thr Glu Ser Arg Phe Ala Thr Asn 
                 35                  40                  45              
         
         
         Thr Thr Leu Ala Gly Gly Leu Asp Val Val Asn Leu Ile Ser Met Ala 
             50                  55                  60                  
         
         
         Lys Arg 
         65      
         
         
         <210>  9
         <211>  432
         <212>  DNA
         <213>  synthetic
         
         
         <220>
         <221>  misc_feature
         <222>  (364)..(365)
         <223>  n is a, c, g, or t
         
         <400>  9
         atgagatttc cttcaatttt tactgcagtt ttattcgcag catcctccgc attagctgct       60
         
         ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt      120
         
         tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat      180
         
         aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta      240
         
         tccatggcta agagagaaga aggtgaacca aagttcgtta accaacactt gtgcggttcc      300
         
         cacttggttg aagctttgta cttggtttgc ggtgaaagag gtttcttcta cactcctaag      360
         
         gctnnkaagg gtattgtcga acaatgctgt acctccatct gctccttgta ccaattggaa      420
         
         aactactgca ac                                                          432
         
         
         <210>  10
         <211>  144
         <212>  PRT
         <213>  synthetic
         
         
         <220>
         <221>  misc_feature
         <222>  (122)..(122)
         <223>  Xaa can be any naturally occurring amino acid
         
         <400>  10
         
         Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
         1               5                   10                  15      
         
         
         Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
                     20                  25                  30          
         
         
         Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
                 35                  40                  45              
         
         
         Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
             50                  55                  60                  
         
         
         Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
         65                  70                  75                  80  
         
         
         Ser Met Ala Lys Arg Glu Glu Gly Glu Pro Lys Phe Val Asn Gln His 
                         85                  90                  95      
         
         
         Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu 
                     100                 105                 110         
         
         
         Arg Gly Phe Phe Tyr Thr Pro Lys Ala Xaa Lys Gly Ile Val Glu Gln 
                 115                 120                 125             
         
         
         Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
             130                 135                 140                 
         
         
         <210>  11
         <211>  375
         <212>  DNA
         <213>  synthetic
         
         <400>  11
         atgaaactga aaactgtaag atctgcggtc ctttcgtcac tctttgcatc tcaggtcctt       60
         
         ggccaaccaa ttgacgacac tgaatctaac actacttctg tcaacttgat ggctgacgac      120
         
         actgaatcca gattcgctac taacactact ttggctggtg gtttggatgt tgttaacttg      180
         
         atctccatgg ctaagagaga agaaggtgaa ccaaagttcg ttaaccaaca cttgtgcggt      240
         
         tcccacttgg ttgaagcttt gtacttggtt tgtggtgaaa gaggtttctt ctacactcct      300
         
         aaggctatga agggtattgt cgaacaatgc tgtacctcca tctgctcctt gtaccaattg      360
         
         gaaaactact gcaac                                                       375
         
         
         <210>  12
         <211>  125
         <212>  PRT
         <213>  synthetic
         
         <400>  12
         
         Met Lys Leu Lys Thr Val Arg Ser Ala Val Leu Ser Ser Leu Phe Ala 
         1               5                   10                  15      
         
         
         Ser Gln Val Leu Gly Gln Pro Ile Asp Asp Thr Glu Ser Asn Thr Thr 
                     20                  25                  30          
         
         
         Ser Val Asn Leu Met Ala Asp Asp Thr Glu Ser Arg Phe Ala Thr Asn 
                 35                  40                  45              
         
         
         Thr Thr Leu Ala Gly Gly Leu Asp Val Val Asn Leu Ile Ser Met Ala 
             50                  55                  60                  
         
         
         Lys Arg Glu Glu Gly Glu Pro Lys Phe Val Asn Gln His Leu Cys Gly 
         65                  70                  75                  80  
         
         
         Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
                         85                  90                  95      
         
         
         Phe Tyr Thr Pro Lys Ala Met Lys Gly Ile Val Glu Gln Cys Cys Thr 
                     100                 105                 110         
         
         
         Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
                 115                 120                 125 
         
         
         <210>  13
         <211>  85
         <212>  PRT
         <213>  S. cerevisiae
         
         <400>  13
         
         Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
         1               5                   10                  15      
         
         
         Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
                     20                  25                  30          
         
         
         Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
                 35                  40                  45              
         
         
         Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
             50                  55                  60                  
         
         
         Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
         65                  70                  75                  80  
         
         
         Ser Met Ala Lys Arg 
                         85  
         
         
         <210>  14
         <211>  85
         <212>  PRT
         <213>  synthetic
         
         <400>  14
         
         Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
         1               5                   10                  15      
         
         
         Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
                     20                  25                  30          
         
         
         Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
                 35                  40                  45              
         
         
         Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
             50                  55                  60                  
         
         
         Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
         65                  70                  75                  80  
         
         
         Ser Leu Asp Lys Arg 
                         85  


              







        

        
