                         SEQUENCE LISTING
<110>  Evonik Industries AG
 
<120>  Optimiertes Verfahren zur Herstellung von Feinchemikalien unter 
       Verwendung eines durch Propionat induzierbaren Promotors

<130>  201100446

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  177
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  repeat_region
<222>  (22)..(49)
<223>  IR1

<220>
<221>  repeat_region
<222>  (77)..(105)
<223>  IR2

<400>  1
ctccagcgtc cacgaatatg cccccgcgcg ccgggtgggg agcgaaggga acccccaagg       60

aattggcgtt gaggtggcga ttttgcatgt tttactcaaa attactttgg tggtcacaaa      120

attacacaac ttttacagtg acctagatcg ctttttaaag aattagcgtg gtgtgca         177


<210>  2
<211>  177
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  repeat_region
<222>  (22)..(49)
<223>  IR1

<220>
<221>  repeat_region
<222>  (77)..(105)
<223>  IR2

<400>  2
ctccagcgtc caagaatatg cccccgcgcg ccgggtgggg agcgaaggga acccccaagg       60

aattggcgtt gaggtggtga ttttgcatgt tttactcaaa atcactttga tggtcacaaa      120

attacacaac ttttacagtg acctacattg ctttttaaag aattagtgtg gtgtgca         177


<210>  3
<211>  177
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  repeat_region
<222>  (22)..(49)
<223>  IR1

<220>
<221>  repeat_region
<222>  (77)..(105)
<223>  IR2

<400>  3
ctccagcgtc caagaatatg cccccgcgcg ccgggtgggg agcgaaggga acccccaagg       60

aattggcgtt gaggtggcga ttttgcatgt tttactcaaa atcactgtga tggtcacaaa      120

attacacaac ttttacagtg acctagatcg ctttttaaag aattagcgtg gtgtgca         177


<210>  4
<211>  902
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  promoter
<222>  (1)..(177)
<223>  PprpD2

<220>
<221>  RBS
<222>  (178)..(206)
<223>  gap RBS

<220>
<221>  misc_feature
<222>  (207)..(209)
<223>  atg-Startcodon

<220>
<221>  gene
<222>  (207)..(902)
<223>  ilvB-Kodierregion 5-Region

<400>  4
ctccagcgtc cacgaatatg cccccgcgcg ccgggtgggg agcgaaggga acccccaagg       60

aattggcgtt gaggtggcga ttttgcatgt tttactcaaa attactttgg tggtcacaaa      120

attacacaac ttttacagtg acctagatcg ctttttaaag aattagcgtg gtgtgcatgc      180

atatttatcg cgagaggaga cacaacatga atgtggcagc ttctcaacag cccactcccg      240

ccacggttgc aagccgtggt cgatccgccg cccctgagcg gatgacaggt gcacaggcaa      300

ttgttcgatc gctcgaggag cttaacgccg acatcgtgtt cggtattcct ggtggtgcgg      360

tgctaccggt gtatgacccg ctctattcct ccacaaaggt gcgccacgtc ctggtgcgcc      420

acgagcaggg cgcaggccac gcagcaaccg gctacgcgca ggttactgga cgcgttggcg      480

tctgcattgc aacctctggc ccaggcgcaa ccaacttggt taccccaatc gctgatgcaa      540

acttggactc cgttcccatg gttgccatca ccggccaggt cggaagtggc ctgctgggta      600

ccgatgcttt ccaggaagcc gatatccgcg gcatcaccat gccagtgacc aagcacaact      660

tcatggtcac cgaccccaac gacattccac aggcattggc tgaggcattc cacctcgcga      720

ttactggtcg ccctggccct gttctggtgg atattcctaa ggatgtccaa aacgctgaat      780

tggatttcgt ctggccacca aagatcgacc tgccaggcta ccgcccagtt tctactccgc      840

atgctcgaca gattgagcag gctgtcaaac tgatcggtga agccaaaaag ccagtccttt      900

ac                                                                     902


<210>  5
<211>  1390
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  misc_feature
<222>  (494)..(521)
<223>  IR1

<220>
<221>  misc_feature
<222>  (549)..(577)
<223>  IR2

<220>
<221>  RBS
<222>  (665)..(678)
<223>  RBS gap

<220>
<221>  gene
<222>  (679)..(1380)
<223>  ilvB 5-region

<400>  5
cgaattcgtg caatcgtgcg aattttggtc gcgcgcatgg tgatctcaat gacggtgcct       60

tcgacaacga tgccgttgcc ctcaaaacgc acccagtcac ccacgccgaa ttgcttttcc      120

gtcaggatga aaaatccggc caagaagtcc gcaacaatcg actgcgcacc aaggccaatg      180

gcagctgacg caatggttgc cggaatcgca gcgcccgcga gagagaaacc aaaagcctgc      240

atcgcggaga cggcaagcat gaaaaacgcc acaatttgcg cgatataaac gccaacgcca      300

gcgaacgcga gctggttctt agtggtgtcc gcatcggctg cagactccac tcgccgcttg      360

ataatacgca tggccagtcg gccgatacgt ggaatcaaaa acgccaagac caggataatt      420

gctacatcaa aaccggtatc gacaatccaa ttccacaatg aatagagcaa atctccagcg      480

tccacgaata tgcccccgcg cgccgggtgg ggagcgaagg gaacccccaa ggaattggcg      540

ttgaggtggc gattttgcat gttttactca aaattacttt ggtggtcaca aaattacaca      600

acttttacag tgacctagat cgctttttaa agaattagcg tggtgtgcat gcatatttat      660

cgcgagagga gacacaacat gaatgtggca gcttctcaac agcccactcc cgccacggtt      720

gcaagccgtg gtcgatccgc cgcccctgag cggatgacag gtgcacaggc aattgttcga      780

tcgctcgagg agcttaacgc cgacatcgtg ttcggtattc ctggtggtgc ggtgctaccg      840

gtgtatgacc cgctctattc ctccacaaag gtgcgccacg tcctggtgcg ccacgagcag      900

ggcgcaggcc acgcagcaac cggctacgcg caggttactg gacgcgttgg cgtctgcatt      960

gcaacctctg gcccaggcgc aaccaacttg gttaccccaa tcgctgatgc aaacttggac     1020

tccgttccca tggttgccat caccggccag gtcggaagtg gcctgctggg taccgatgct     1080

ttccaggaag ccgatatccg cggcatcacc atgccagtga ccaagcacaa cttcatggtc     1140

accgacccca acgacattcc acaggcattg gctgaggcat tccacctcgc gattactggt     1200

cgccctggcc ctgttctggt ggatattcct aaggatgtcc aaaacgctga attggatttc     1260

gtctggccac caaagatcga cctgccaggc taccgcccag tttctactcc gcatgctcga     1320

cagattgagc aggctgtcaa actgatcggt gaagccaaaa agccagtcct ttacattggc     1380

ggcaagcttg                                                            1390


<210>  6
<211>  20
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Testprimer 1

<400>  6
aaagcctgca tcgcggagac                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Testprimer 2

<400>  7
tggtgatgcc gcggatatcg                                                   20


<210>  8
<211>  3411
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  CDS
<222>  (500)..(2380)
<223>  Kodierregion ilvB

<220>
<221>  CDS
<222>  (2394)..(2912)
<223>  Kodierregion ilvN

<400>  8
gtatcgacaa tccaattcca caatgaatag agcaaatatt gaatgggtac gcctaaaatc       60

atgagccaag attagcgctg aaaagtagcg ggagcctgcc tgaactttgt gagaatcctg      120

attccttaac cgaagtgggg gagttttggg ggtgggaatt ttcgtgcgtt gtggaattgg      180

aaactcgatg tgtgtagcat gacacaccat gaccattatt cgacttgtag tagtaaccgc      240

gcggcgcctg ccgtaacggc cttccaagtc gtctcgtcaa gcgccctcga caacactcac      300

cacagtgttg gaacgagggc tttcttgttg gttatgaccc aagtagccaa ctttgcaaca      360

gacatctgtc gcactgcgtg cacacgcatc cgcgtcggaa caattttaaa tgagggcttt      420

gtctttaggc tgagttgaaa tcggcttggc ttggacgggt cctgtgaaaa tccttattta      480

gtaaaggagc cagaaagtc gtg aat gtg gca gct tct caa cag ccc act ccc       532
                     Val Asn Val Ala Ala Ser Gln Gln Pro Thr Pro          
                     1               5                   10               

gcc acg gtt gca agc cgt ggt cga tcc gcc gcc cct gag cgg atg aca        580
Ala Thr Val Ala Ser Arg Gly Arg Ser Ala Ala Pro Glu Arg Met Thr           
            15                  20                  25                    

ggt gca aag gca att gtt cga tcg ctc gag gag ctt aac gcc gac atc        628
Gly Ala Lys Ala Ile Val Arg Ser Leu Glu Glu Leu Asn Ala Asp Ile           
        30                  35                  40                        

gtg ttc ggt att cct ggt ggt gcg gtg cta ccg gtg tat gac ccg ctc        676
Val Phe Gly Ile Pro Gly Gly Ala Val Leu Pro Val Tyr Asp Pro Leu           
    45                  50                  55                            

tat tcc tcc aca aag gtg cgc cac gtc ttg gtg cgc cac gag cag ggc        724
Tyr Ser Ser Thr Lys Val Arg His Val Leu Val Arg His Glu Gln Gly           
60                  65                  70                  75            

gca ggc cac gca gca acc ggc tac gcg cag gtt act gga cgc gtt ggc        772
Ala Gly His Ala Ala Thr Gly Tyr Ala Gln Val Thr Gly Arg Val Gly           
                80                  85                  90                

gtc tgc att gca acc tct ggc cca gga gca acc aac ttg gtt acc cca        820
Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val Thr Pro           
            95                  100                 105                   

atc gct gat gca aac ttg gac tcc gtt ccc atg gtt gcc atc acc ggc        868
Ile Ala Asp Ala Asn Leu Asp Ser Val Pro Met Val Ala Ile Thr Gly           
        110                 115                 120                       

cag gtc gga agt ggc ctg ctg ggt acc gac gct ttc cag gaa gcc gat        916
Gln Val Gly Ser Gly Leu Leu Gly Thr Asp Ala Phe Gln Glu Ala Asp           
    125                 130                 135                           

atc cgc ggc atc acc atg cca gtg acc aag cac aac ttc atg gtc acc        964
Ile Arg Gly Ile Thr Met Pro Val Thr Lys His Asn Phe Met Val Thr           
140                 145                 150                 155           

aac cct aac gac att cca cag gca ttg gct gag gca ttc cac ctc gcg       1012
Asn Pro Asn Asp Ile Pro Gln Ala Leu Ala Glu Ala Phe His Leu Ala           
                160                 165                 170               

att act ggt cgc cct ggc cct gtt ctg gtg gat att cct aag gat gtc       1060
Ile Thr Gly Arg Pro Gly Pro Val Leu Val Asp Ile Pro Lys Asp Val           
            175                 180                 185                   

cag aac gct gaa ttg gat ttc gtc tgg cca cca aag atc gac ctg cca       1108
Gln Asn Ala Glu Leu Asp Phe Val Trp Pro Pro Lys Ile Asp Leu Pro           
        190                 195                 200                       

ggc tac cgc cca gtt tca aca cca cat gct cgc cag atc gag cag gca       1156
Gly Tyr Arg Pro Val Ser Thr Pro His Ala Arg Gln Ile Glu Gln Ala           
    205                 210                 215                           

gtc aag ctg atc ggt gag gcc aag aag ccc gtc ctt tac gtt ggt ggt       1204
Val Lys Leu Ile Gly Glu Ala Lys Lys Pro Val Leu Tyr Val Gly Gly           
220                 225                 230                 235           

ggc gta atc aag gct gac gca cac gaa gag ctt cgt gcg ttc gct gag       1252
Gly Val Ile Lys Ala Asp Ala His Glu Glu Leu Arg Ala Phe Ala Glu           
                240                 245                 250               

tac acc ggc atc cca gtt gtc acc acc ttg atg gct ttg ggt act ttc       1300
Tyr Thr Gly Ile Pro Val Val Thr Thr Leu Met Ala Leu Gly Thr Phe           
            255                 260                 265                   

cca gag tct cac gag ctg cac atg ggt atg cca ggc atg cat ggc act       1348
Pro Glu Ser His Glu Leu His Met Gly Met Pro Gly Met His Gly Thr           
        270                 275                 280                       

gtg tcc gct gtt ggt gca ctg cag cgc agc gac ctg ctg att gct atc       1396
Val Ser Ala Val Gly Ala Leu Gln Arg Ser Asp Leu Leu Ile Ala Ile           
    285                 290                 295                           

ggc tcc cgc ttt gat gac cgc gtc acc ggt gac gtt gac acc ttc gcg       1444
Gly Ser Arg Phe Asp Asp Arg Val Thr Gly Asp Val Asp Thr Phe Ala           
300                 305                 310                 315           

cct gac gcc aag atc att cac gcc gac att gat cct gcc gaa atc ggc       1492
Pro Asp Ala Lys Ile Ile His Ala Asp Ile Asp Pro Ala Glu Ile Gly           
                320                 325                 330               

aag atc aag cag gtt gag gtt cca atc gtg ggc gat gcc cgc gaa gtt       1540
Lys Ile Lys Gln Val Glu Val Pro Ile Val Gly Asp Ala Arg Glu Val           
            335                 340                 345                   

ctt gct cgt ctg ctg gaa acc acc aag gca agc aag gca gag acc gag       1588
Leu Ala Arg Leu Leu Glu Thr Thr Lys Ala Ser Lys Ala Glu Thr Glu           
        350                 355                 360                       

gac atc tcc gag tgg gtt gac tac ctc aag ggc ctc aag gca cgt ttc       1636
Asp Ile Ser Glu Trp Val Asp Tyr Leu Lys Gly Leu Lys Ala Arg Phe           
    365                 370                 375                           

ccg cgt ggc tac gac gag cag cca ggc gat ctg ctg gca cca cag ttt       1684
Pro Arg Gly Tyr Asp Glu Gln Pro Gly Asp Leu Leu Ala Pro Gln Phe           
380                 385                 390                 395           

gtc att gaa acc ctg tcc aag gaa gtt ggc ccc gac gca att tac tgc       1732
Val Ile Glu Thr Leu Ser Lys Glu Val Gly Pro Asp Ala Ile Tyr Cys           
                400                 405                 410               

gcc ggc gtt ggc cag cac caa atg tgg gca gct cag ttc gtt gac ttt       1780
Ala Gly Val Gly Gln His Gln Met Trp Ala Ala Gln Phe Val Asp Phe           
            415                 420                 425                   

gaa aag cca cgc acc tgg ctc aac tcc ggt gga ctg ggc acc atg ggc       1828
Glu Lys Pro Arg Thr Trp Leu Asn Ser Gly Gly Leu Gly Thr Met Gly           
        430                 435                 440                       

tac gca gtt cct gcg gcc ctt gga gca aag gct ggc gca cct gac aag       1876
Tyr Ala Val Pro Ala Ala Leu Gly Ala Lys Ala Gly Ala Pro Asp Lys           
    445                 450                 455                           

gaa gtc tgg gct atc gac ggc gac ggc tgt ttc cag atg acc aac cag       1924
Glu Val Trp Ala Ile Asp Gly Asp Gly Cys Phe Gln Met Thr Asn Gln           
460                 465                 470                 475           

gaa ctc acc acc gcc gca gtt gaa ggt ttc ccc att aag atc gca cta       1972
Glu Leu Thr Thr Ala Ala Val Glu Gly Phe Pro Ile Lys Ile Ala Leu           
                480                 485                 490               

atc aac aac gga aac ctg ggc atg gtt cgc caa tgg cag acc cta ttc       2020
Ile Asn Asn Gly Asn Leu Gly Met Val Arg Gln Trp Gln Thr Leu Phe           
            495                 500                 505                   

tat gaa gga cgg tac tca aat act aaa ctt cgt aac cag ggc gag tac       2068
Tyr Glu Gly Arg Tyr Ser Asn Thr Lys Leu Arg Asn Gln Gly Glu Tyr           
        510                 515                 520                       

atg ccc gac ttt gtt acc ctt tct gag gga ctt ggc tgt gtt gcc atc       2116
Met Pro Asp Phe Val Thr Leu Ser Glu Gly Leu Gly Cys Val Ala Ile           
    525                 530                 535                           

cgc gtc acc aaa gcg gag gaa gta ctg cca gcc atc caa aag gct cga       2164
Arg Val Thr Lys Ala Glu Glu Val Leu Pro Ala Ile Gln Lys Ala Arg           
540                 545                 550                 555           

gag atc aac gac cgc cca gta gtc atc gac ttc atc gtc ggt gaa gac       2212
Glu Ile Asn Asp Arg Pro Val Val Ile Asp Phe Ile Val Gly Glu Asp           
                560                 565                 570               

gca cag gta tgg cca atg gtg tct gct gga tca tcc aac tcc gat atc       2260
Ala Gln Val Trp Pro Met Val Ser Ala Gly Ser Ser Asn Ser Asp Ile           
            575                 580                 585                   

cag tac gca ctc gga ttg cgc cca ttc ttt gat ggt gat gaa tct gca       2308
Gln Tyr Ala Leu Gly Leu Arg Pro Phe Phe Asp Gly Asp Glu Ser Ala           
        590                 595                 600                       

gca gaa gat cct gcc gac att cac gaa gcc gtc agc gac att gat gcc       2356
Ala Glu Asp Pro Ala Asp Ile His Glu Ala Val Ser Asp Ile Asp Ala           
    605                 610                 615                           

gcc gtt gaa tcg acc gag gca taa ggagagaccc aag atg gct aat tct        2405
Ala Val Glu Ser Thr Glu Ala                    Met Ala Asn Ser            
620                 625                                    630            

gac gtc acc cgc cac atc ctg tcc gta ctc gtt cag gac gta gac gga       2453
Asp Val Thr Arg His Ile Leu Ser Val Leu Val Gln Asp Val Asp Gly           
                635                 640                 645               

atc att tcc cgc gta tca ggt atg ttc acc cga cgc gca ttc aac ctc       2501
Ile Ile Ser Arg Val Ser Gly Met Phe Thr Arg Arg Ala Phe Asn Leu           
            650                 655                 660                   

gtg tcc ctc gtg tct gca aag acc gaa aca cac ggc atc aac cgc atc       2549
Val Ser Leu Val Ser Ala Lys Thr Glu Thr His Gly Ile Asn Arg Ile           
        665                 670                 675                       

acg gtt gtt gtc gac gcc gac gag ctc aac att gag cag atc acc aag       2597
Thr Val Val Val Asp Ala Asp Glu Leu Asn Ile Glu Gln Ile Thr Lys           
    680                 685                 690                           

cag ctc aac aag ctg atc ccc gtg ctc aaa gtc gtg cga ctt gat gaa       2645
Gln Leu Asn Lys Leu Ile Pro Val Leu Lys Val Val Arg Leu Asp Glu           
695                 700                 705                 710           

gag acc act atc gcc cgc gca atc atg ctg gtt aag gtc tct gcg gac       2693
Glu Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys Val Ser Ala Asp           
                715                 720                 725               

agc acc aac cgt ccg cag atc gtc gac gcc gcg aac atc ttc cgc gcc       2741
Ser Thr Asn Arg Pro Gln Ile Val Asp Ala Ala Asn Ile Phe Arg Ala           
            730                 735                 740                   

cga gtc gtc gac gtg gct cca gac tct gtg gtt att gaa tcc aca ggc       2789
Arg Val Val Asp Val Ala Pro Asp Ser Val Val Ile Glu Ser Thr Gly           
        745                 750                 755                       

acc cca ggc aag ctc cgc gca ctg ctt gac gtg atg gaa cca ttc gga       2837
Thr Pro Gly Lys Leu Arg Ala Leu Leu Asp Val Met Glu Pro Phe Gly           
    760                 765                 770                           

atc cgc gaa ctg atc caa tcc gga cag att gca ctc aac cgc ggt ccg       2885
Ile Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu Asn Arg Gly Pro           
775                 780                 785                 790           

aag acc atg gct ccg gcc aag atc taa acagcaatta atctgattgc             2932
Lys Thr Met Ala Pro Ala Lys Ile                                           
                795                                                       

acctgctgca taaatgtgac tagtcaaaca ccgtctaatt acatgtgtgt ggtagaacaa     2992

taatgtagtt gtctgcccaa gcgagttaaa ctcccacgat ttacagtggg gggcagacat     3052

cttttcacca aaatttttac gaaaggcgag attttctccc atggctattg aactgcttta     3112

tgatgctgac gctgacctct ccttgatcca gggccgtaag gttgccatcg ttggctacgg     3172

ctcccagggc cacgcacact cccagaacct ccgcgattct ggcgttgagg ttgtcattgg     3232

tctgcgcgag ggctccaagt ccgcagagaa ggcaaaggaa gcaggcttcg aggtcaagac     3292

caccgctgag gctgcagctt gggctgacgt catcatgctc ctggctccag acacctccca     3352

ggcagaaatc ttcaccaacg acatcgagcc aaacctgaac gcaggcgacg cactgctgt      3411


<210>  9
<211>  626
<212>  PRT
<213>  Corynebacterium glutamicum

<400>  9

Val Asn Val Ala Ala Ser Gln Gln Pro Thr Pro Ala Thr Val Ala Ser 
1               5                   10                  15      


Arg Gly Arg Ser Ala Ala Pro Glu Arg Met Thr Gly Ala Lys Ala Ile 
            20                  25                  30          


Val Arg Ser Leu Glu Glu Leu Asn Ala Asp Ile Val Phe Gly Ile Pro 
        35                  40                  45              


Gly Gly Ala Val Leu Pro Val Tyr Asp Pro Leu Tyr Ser Ser Thr Lys 
    50                  55                  60                  


Val Arg His Val Leu Val Arg His Glu Gln Gly Ala Gly His Ala Ala 
65                  70                  75                  80  


Thr Gly Tyr Ala Gln Val Thr Gly Arg Val Gly Val Cys Ile Ala Thr 
                85                  90                  95      


Ser Gly Pro Gly Ala Thr Asn Leu Val Thr Pro Ile Ala Asp Ala Asn 
            100                 105                 110         


Leu Asp Ser Val Pro Met Val Ala Ile Thr Gly Gln Val Gly Ser Gly 
        115                 120                 125             


Leu Leu Gly Thr Asp Ala Phe Gln Glu Ala Asp Ile Arg Gly Ile Thr 
    130                 135                 140                 


Met Pro Val Thr Lys His Asn Phe Met Val Thr Asn Pro Asn Asp Ile 
145                 150                 155                 160 


Pro Gln Ala Leu Ala Glu Ala Phe His Leu Ala Ile Thr Gly Arg Pro 
                165                 170                 175     


Gly Pro Val Leu Val Asp Ile Pro Lys Asp Val Gln Asn Ala Glu Leu 
            180                 185                 190         


Asp Phe Val Trp Pro Pro Lys Ile Asp Leu Pro Gly Tyr Arg Pro Val 
        195                 200                 205             


Ser Thr Pro His Ala Arg Gln Ile Glu Gln Ala Val Lys Leu Ile Gly 
    210                 215                 220                 


Glu Ala Lys Lys Pro Val Leu Tyr Val Gly Gly Gly Val Ile Lys Ala 
225                 230                 235                 240 


Asp Ala His Glu Glu Leu Arg Ala Phe Ala Glu Tyr Thr Gly Ile Pro 
                245                 250                 255     


Val Val Thr Thr Leu Met Ala Leu Gly Thr Phe Pro Glu Ser His Glu 
            260                 265                 270         


Leu His Met Gly Met Pro Gly Met His Gly Thr Val Ser Ala Val Gly 
        275                 280                 285             


Ala Leu Gln Arg Ser Asp Leu Leu Ile Ala Ile Gly Ser Arg Phe Asp 
    290                 295                 300                 


Asp Arg Val Thr Gly Asp Val Asp Thr Phe Ala Pro Asp Ala Lys Ile 
305                 310                 315                 320 


Ile His Ala Asp Ile Asp Pro Ala Glu Ile Gly Lys Ile Lys Gln Val 
                325                 330                 335     


Glu Val Pro Ile Val Gly Asp Ala Arg Glu Val Leu Ala Arg Leu Leu 
            340                 345                 350         


Glu Thr Thr Lys Ala Ser Lys Ala Glu Thr Glu Asp Ile Ser Glu Trp 
        355                 360                 365             


Val Asp Tyr Leu Lys Gly Leu Lys Ala Arg Phe Pro Arg Gly Tyr Asp 
    370                 375                 380                 


Glu Gln Pro Gly Asp Leu Leu Ala Pro Gln Phe Val Ile Glu Thr Leu 
385                 390                 395                 400 


Ser Lys Glu Val Gly Pro Asp Ala Ile Tyr Cys Ala Gly Val Gly Gln 
                405                 410                 415     


His Gln Met Trp Ala Ala Gln Phe Val Asp Phe Glu Lys Pro Arg Thr 
            420                 425                 430         


Trp Leu Asn Ser Gly Gly Leu Gly Thr Met Gly Tyr Ala Val Pro Ala 
        435                 440                 445             


Ala Leu Gly Ala Lys Ala Gly Ala Pro Asp Lys Glu Val Trp Ala Ile 
    450                 455                 460                 


Asp Gly Asp Gly Cys Phe Gln Met Thr Asn Gln Glu Leu Thr Thr Ala 
465                 470                 475                 480 


Ala Val Glu Gly Phe Pro Ile Lys Ile Ala Leu Ile Asn Asn Gly Asn 
                485                 490                 495     


Leu Gly Met Val Arg Gln Trp Gln Thr Leu Phe Tyr Glu Gly Arg Tyr 
            500                 505                 510         


Ser Asn Thr Lys Leu Arg Asn Gln Gly Glu Tyr Met Pro Asp Phe Val 
        515                 520                 525             


Thr Leu Ser Glu Gly Leu Gly Cys Val Ala Ile Arg Val Thr Lys Ala 
    530                 535                 540                 


Glu Glu Val Leu Pro Ala Ile Gln Lys Ala Arg Glu Ile Asn Asp Arg 
545                 550                 555                 560 


Pro Val Val Ile Asp Phe Ile Val Gly Glu Asp Ala Gln Val Trp Pro 
                565                 570                 575     


Met Val Ser Ala Gly Ser Ser Asn Ser Asp Ile Gln Tyr Ala Leu Gly 
            580                 585                 590         


Leu Arg Pro Phe Phe Asp Gly Asp Glu Ser Ala Ala Glu Asp Pro Ala 
        595                 600                 605             


Asp Ile His Glu Ala Val Ser Asp Ile Asp Ala Ala Val Glu Ser Thr 
    610                 615                 620                 


Glu Ala 
625     


<210>  10
<211>  172
<212>  PRT
<213>  Corynebacterium glutamicum

<400>  10

Met Ala Asn Ser Asp Val Thr Arg His Ile Leu Ser Val Leu Val Gln 
1               5                   10                  15      


Asp Val Asp Gly Ile Ile Ser Arg Val Ser Gly Met Phe Thr Arg Arg 
            20                  25                  30          


Ala Phe Asn Leu Val Ser Leu Val Ser Ala Lys Thr Glu Thr His Gly 
        35                  40                  45              


Ile Asn Arg Ile Thr Val Val Val Asp Ala Asp Glu Leu Asn Ile Glu 
    50                  55                  60                  


Gln Ile Thr Lys Gln Leu Asn Lys Leu Ile Pro Val Leu Lys Val Val 
65                  70                  75                  80  


Arg Leu Asp Glu Glu Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys 
                85                  90                  95      


Val Ser Ala Asp Ser Thr Asn Arg Pro Gln Ile Val Asp Ala Ala Asn 
            100                 105                 110         


Ile Phe Arg Ala Arg Val Val Asp Val Ala Pro Asp Ser Val Val Ile 
        115                 120                 125             


Glu Ser Thr Gly Thr Pro Gly Lys Leu Arg Ala Leu Leu Asp Val Met 
    130                 135                 140                 


Glu Pro Phe Gly Ile Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu 
145                 150                 155                 160 


Asn Arg Gly Pro Lys Thr Met Ala Pro Ala Lys Ile 
                165                 170         


<210>  11
<211>  28
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  repeat_region
<222>  (1)..(28)
<223>  IR1

<400>  11
ccccgcgcgc cgggtgggga gcgaaggg                                          28


<210>  12
<211>  29
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  repeat_region
<222>  (1)..(29)
<223>  IR2

<400>  12
gcgattttgc atgttttact caaaattac                                         29


<210>  13
<211>  2619
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  promoter
<222>  (1)..(177)
<223>  PprpD2

<220>
<221>  RBS
<222>  (178)..(206)
<223>  RBS gap

<220>
<221>  misc_feature
<222>  (207)..(209)
<223>  atg-Startcodon

<220>
<221>  gene
<222>  (207)..(2087)
<223>  Kodierregion ilvB

<220>
<221>  gene
<222>  (2101)..(2619)
<223>  Kodierregion ilvN

<400>  13
ctccagcgtc cacgaatatg cccccgcgcg ccgggtgggg agcgaaggga acccccaagg       60

aattggcgtt gaggtggcga ttttgcatgt tttactcaaa attactttgg tggtcacaaa      120

attacacaac ttttacagtg acctagatcg ctttttaaag aattagcgtg gtgtgcatgc      180

atatttatcg cgagaggaga cacaacatga atgtggcagc ttctcaacag cccactcccg      240

ccacggttgc aagccgtggt cgatccgccg cccctgagcg gatgacaggt gcacaggcaa      300

ttgttcgatc gctcgaggag cttaacgccg acatcgtgtt cggtattcct ggtggtgcgg      360

tgctaccggt gtatgacccg ctctattcct ccacaaaggt gcgccacgtc ctggtgcgcc      420

acgagcaggg cgcaggccac gcagcaaccg gctacgcgca ggttactgga cgcgttggcg      480

tctgcattgc aacctctggc ccaggcgcaa ccaacttggt taccccaatc gctgatgcaa      540

acttggactc cgttcccatg gttgccatca ccggccaggt cggaagtggc ctgctgggta      600

ccgatgcttt ccaggaagcc gatatccgcg gcatcaccat gccagtgacc aagcacaact      660

tcatggtcac cgaccccaac gacattccac aggcattggc tgaggcattc cacctcgcga      720

ttactggtcg ccctggccct gttctggtgg atattcctaa ggatgtccaa aacgctgaat      780

tggatttcgt ctggccacca aagatcgacc tgccaggcta ccgcccagtt tctactccgc      840

atgctcgaca gattgagcag gctgtcaaac tgatcggtga agccaaaaag ccagtccttt      900

acgttggtgg tggcgtaatc aaggctgacg cacacgaaga gcttcgtgcg ttcgctgagt      960

acaccggcat cccagttgtc accaccttga tggctttggg tactttccca gagtctcacg     1020

agctgcacat gggtatgcca ggcatgcatg gcactgtgtc cgctgttggt gcactgcagc     1080

gcagcgacct gctgattgct atcggctccc gctttgatga ccgcgtcacc ggtgacgttg     1140

acaccttcgc gcctgacgcc aagatcattc acgccgacat tgatcctgcc gaaatcggca     1200

agatcaagca ggttgaggtt ccaatcgtgg gcgatgcccg cgaagttctt gctcgtctgc     1260

tggaaaccac caaggcaagc aaggcagaga ccgaggacat ctccgagtgg gttgactacc     1320

tcaagggcct caaggcacgt ttcccgcgtg gctacgacga gcagccaggc gatctgctgg     1380

caccacagtt tgtcattgaa accctgtcca aggaagttgg ccccgacgca atttactgcg     1440

ccggcgttgg ccagcaccaa atgtgggcag ctcagttcgt tgactttgaa aagccacgca     1500

cctggctcaa ctccggtgga ctgggcacca tgggctacgc agttcctgcg gcccttggag     1560

caaaggctgg cgcacctgac aaggaagtct gggctatcga cggcgacggc tgtttccaga     1620

tgaccaacca ggaactcacc accgccgcag ttgaaggttt ccccattaag atcgcactaa     1680

tcaacaacgg aaacctgggc atggttcgcc aatggcagac cctattctat gaaggacggt     1740

actcaaatac taaacttcgt aaccagggcg agtacatgcc cgactttgtt accctttctg     1800

agggacttgg ctgtgttgcc atccgcgtca ccaaagcgga ggaagtactg ccagccatcc     1860

aaaaggctcg agagatcaac gaccgcccag tagtcatcga cttcatcgtc ggtgaagacg     1920

cacaggtatg gccaatggtg tctgctggat catccaactc cgatatccag tacgcactcg     1980

gattgcgccc attctttgat ggtgatgaat ctgcagcaga agatcctgcc gacattcacg     2040

aagccgtcag cgacattgat gccgccgttg aatcgaccga ggcataagga gagacccaag     2100

atggctaatt ctgacgtcac ccgccacatc ctgtccgtac tcgttcagga cgtagacgga     2160

atcatttccc gcgtatcagg tatgttcacc cgacgcgcat tcaacctcgt gtccctcgtg     2220

tctgcaaaga ccgaaacaca cggcatcaac cgcatcacgg ttgttgtcga cgccgacgag     2280

ctcaacattg agcagatcac caagcagctc aacaagctga tccccgtgct caaagtcgtg     2340

cgacttgatg aagagaccac tatcgcccgc gcaatcatgc tggttaaggt ctctgcggac     2400

agcaccaacc gtccgcagat cgtcgacgcc gcgaacatct tccgcgcccg agtcgtcgac     2460

gtggctccag actctgtggt tattgaatcc acaggcaccc caggcaagct ccgcgcactg     2520

cttgacgtga tggaaccatt cggaatccgc gaactgatcc aatccggaca gattgcactc     2580

aaccgcggtc cgaagaccat ggctccggcc aagatctaa                            2619


<210>  14
<211>  1421
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  gene
<222>  (7)..(636)
<223>  3-Bereich des ilvB-Gens

<220>
<221>  gene
<222>  (650)..(1168)
<223>  ilvN-Gen

<220>
<221>  misc_feature
<222>  (707)..(715)
<223>  mutierter Genabschnitt

<400>  14
gaattccaga tgtgggcagc tcagttcgtt gactttgaaa agccacgcac ctggctcaac       60

tctggcggcc tgggcaccat gggctacgca gttcctgcgg ctcttggagc aaaggctggc      120

gcacctgaca aggaagtctg ggctatcgac ggcgacggct gtttccagat gaccaaccag      180

gaactcacca ccgccgcagt tgaaggtttc cccattaaga tcgcactaat caacaacgga      240

aacctgggca tggttcgcca atggcagacc ctattctatg aaggacggta ctcaaatact      300

aaacttcgta accagggcga gtacatgccc gactttgtta ccctttctga gggacttggc      360

tgtgttgcca tccgcgtcac caaagcggag gaagtactgc cagccatcca aaaggctcga      420

gagatcaacg accgcccagt agtcatcgac ttcatcgtcg gtgaagacgc acaggtatgg      480

ccaatggtgt ctgctggatc atccaactcc gatatccagt acgcactcgg attgcgccca      540

ttctttgatg gtgatgaatc tgcagcagaa gatcctgccg acattcacga agccgtcagc      600

gacattgatg ccgccgttga atcgaccgag gcataaggag agacccaaga tggctaattc      660

tgacgtcacc cgccacatcc tgtccgtact cgttcaggac gtagacgatg acttttcccg      720

cgtatcaggt atgttcaccc gacgcgcatt caacctcgtg tccctcgtgt ctgcaaagac      780

cgaaacactc ggcatcaacc gcatcacggt tgttgtcgac gccgacgagc tcaacattga      840

gcagatcacc aagcagctca acaagctgat ccccgtgctc aaagtcgtgc gacttgatga      900

agagaccacc atcgcccgcg caatcatgct ggttaaggtc tctgcggata gcaccaaccg      960

tccgcagatc gtcgacgccg cgaacatctt ccgcgcccga gtcgtcgacg tggctccaga     1020

ctctgtggtt attgaatcca caggcacccc aggcaagctc cgcgcactgc ttgatgtgat     1080

ggaaccattc ggaatccgcg aactgatcca atccggacag attgcactca accgcggtcc     1140

gaagaccatg gctccggcca agatctaaac agcaattaat ctgattgcac ctgctgcata     1200

aatgtgacta gtcaaacacc gtctaattgc atgtgtgtgg tagaacaata atgtagttgt     1260

ctgcccaagc gagtttaact cccacgattt acagtggggg cagacatctt ttcaccaaaa     1320

tttttacgaa aggcgagatt ttctcccatg gctattgaac tgctttatga tgctgacgct     1380

gacctctcct tgatccaggg ccgcaaggtt gccataagct t                         1421


<210>  15
<211>  19
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  Testprimer 3

<400>  15
cccagtagtc atcgacttc                                                    19


<210>  16
<211>  20
<212>  DNA
<213>  Corynebacterium glutamicum


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Testprimer 4

<400>  16
cagcgtcagc atcataaagc                                                   20




201100446 AL

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