                         SEQUENCE LISTING

<110>  INMUNOVA S.A.
       Spatz , Martin D. L.
 
<120>  CHIMERAS OF BRUCELLA LUMAZINE SYNTHASE AND BETA SUBUNIT OF AB5 
       TOXINS

<130>  461-2611 PCT

<150>  US 61/827713
<151>  2013-05-27

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  70
<212>  PRT
<213>  Escherichia coli

<400>  1

Ala Asp Cys Ala Lys Gly Lys Ile Glu Phe Ser Lys Tyr Asn Glu Asp 
1               5                   10                  15      


Asp Thr Phe Thr Val Lys Val Asp Gly Lys Glu Tyr Trp Thr Ser Arg 
            20                  25                  30          


Trp Asn Leu Gln Pro Leu Leu Gln Ser Ala Gln Leu Thr Gly Met Thr 
        35                  40                  45              


Val Thr Ile Lys Ser Ser Thr Cys Glu Ser Gly Ser Gly Phe Ala Glu 
    50                  55                  60                  


Val Gln Phe Asn Asn Asp 
65                  70  


<210>  2
<211>  174
<212>  DNA
<213>  Escherichia coli

<400>  2
aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt tgacgggaaa       60

gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca gttgacagga      120

atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc tgaa            174


<210>  3
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3

Met His Ala Asp Cys Ala Lys Gly Lys Ile Glu Phe Ser Lys Tyr Asn 
1               5                   10                  15      


Glu Asp Asp Thr Phe Thr Val Lys Val Asp Gly Lys Glu Tyr Trp Thr 
            20                  25                  30          


Ser Arg Trp Asn Leu Gln Pro Leu Leu Gln Ser Ala Gln Leu Thr Gly 
        35                  40                  45              


Met Thr Val Thr Ile Lys Ser Ser Thr Cys Glu Ser Gly Ser Gly Phe 
    50                  55                  60                  


Ala Glu Val Gln Phe Asn Asn Asp Gly Ser Gly Ser Gly Leu Lys Thr 
65                  70                  75                  80  


Ser Phe Lys Ile Ala Phe Ile Gln Ala Arg Trp His Ala Asp Ile Val 
                85                  90                  95      


Asp Glu Ala Arg Lys Ser Phe Val Ala Glu Leu Ala Ala Lys Thr Gly 
            100                 105                 110         


Gly Ser Val Glu Val Glu Ile Phe Asp Val Pro Gly Ala Tyr Glu Ile 
        115                 120                 125             


Pro Leu His Ala Lys Thr Leu Ala Arg Thr Gly Arg Tyr Ala Ala Ile 
    130                 135                 140                 


Val Gly Ala Ala Phe Val Ile Asp Gly Gly Ile Tyr Arg His Asp Phe 
145                 150                 155                 160 


Val Ala Thr Ala Val Ile Asn Gly Met Met Gln Val Gln Leu Glu Thr 
                165                 170                 175     


Glu Val Pro Val Leu Ser Val Val Leu Thr Pro His His Phe His Glu 
            180                 185                 190         


Ser Lys Glu His His Asp Phe Phe His Ala His Phe Lys Val Lys Gly 
        195                 200                 205             


Val Glu Ala Ala His Ala Ala Leu Gln Ile Val Ser Glu Arg Ser Arg 
    210                 215                 220                 


Ile Ala Leu Val 
225             


<210>  4
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4
atgcatgcgg attgtgctaa aggtaaaatt gagttttcca agtataatga ggatgacaca       60

tttacagtga aggttgacgg gaaagaatac tggaccagtc gctggaatct gcaaccgtta      120

ctgcaaagtg ctcagttgac aggaatgact gtcacaatca aatccagtac ctgtgaatca      180

ggctccggat ttgctgaagt gcagtttaat aatgacggtt ctggttctgg tcttaagaca      240

tcctttaaaa tcgcattcat tcaggcccgc tggcacgccg acatcgttga cgaagcgcgc      300

aaaagctttg tcgccgaact ggccgcaaag acgggtggca gcgtcgaggt agagatattc      360

gacgtgccgg gtgcatatga aattcccctt cacgccaaga cattggccag aaccgggcgc      420

tatgcagcca tcgtcggtgc ggccttcgtg atcgacggcg gcatctatcg tcatgatttc      480

gtggcgacgg ccgttatcaa cggcatgatg caggtgcagc ttgaaacgga agtgccggtg      540

ctgagcgtcg tgctgacgcc gcaccatttc catgaaagca aggagcatca cgacttcttc      600

catgctcatt tcaaggtgaa gggcgtggaa gcggcccatg ccgccttgca gatcgtgagc      660

gagcgcagcc gcatcgcgct tgtctga                                          687


<210>  5
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5

Met His Ala Asp Cys Ala Lys Gly Lys Ile Glu Phe Ser Lys Tyr Asn 
1               5                   10                  15      


Glu Asp Asp Thr Phe Thr Val Lys Val Asp Gly Lys Glu Tyr Trp Thr 
            20                  25                  30          


Ser Arg Trp Asn Leu Gln Pro Leu Leu Gln Ser Ala Gln Leu Thr Gly 
        35                  40                  45              


Met Thr Val Thr Ile Lys Ser Ser Thr Cys Glu Ser Gly Ser Gly Phe 
    50                  55                  60                  


Ala Glu Val Gln Phe Asn Asn Asp Gly Ser Gly Ser Gly Ser Gly Ser 
65                  70                  75                  80  


Gly Ser Leu Lys Thr Ser Phe Lys Ile Ala Phe Ile Gln Ala Arg Trp 
                85                  90                  95      


His Ala Asp Ile Val Asp Glu Ala Arg Lys Ser Phe Val Ala Glu Leu 
            100                 105                 110         


Ala Ala Lys Thr Gly Gly Ser Val Glu Val Glu Ile Phe Asp Val Pro 
        115                 120                 125             


Gly Ala Tyr Glu Ile Pro Leu His Ala Lys Thr Leu Ala Arg Thr Gly 
    130                 135                 140                 


Arg Tyr Ala Ala Ile Val Gly Ala Ala Phe Val Ile Asp Gly Gly Ile 
145                 150                 155                 160 


Tyr Arg His Asp Phe Val Ala Thr Ala Val Ile Asn Gly Met Met Gln 
                165                 170                 175     


Val Gln Leu Glu Thr Glu Val Pro Val Leu Ser Val Val Leu Thr Pro 
            180                 185                 190         


His His Phe His Glu Ser Lys Glu His His Asp Phe Phe His Ala His 
        195                 200                 205             


Phe Lys Val Lys Gly Val Glu Ala Ala His Ala Ala Leu Gln Ile Val 
    210                 215                 220                 


Ser Glu Arg Ser Arg Ile Ala Leu Val 
225                 230             


<210>  6
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6
atgcatgcgg attgtgctaa aggtaaaatt gagttttcca agtataatga ggatgacaca       60

tttacagtga aggttgacgg gaaagaatac tggaccagtc gctggaatct gcaaccgtta      120

ctgcaaagtg ctcagttgac aggaatgact gtcacaatca aatccagtac ctgtgaatca      180

ggctccggat ttgctgaagt gcagtttaat aatgacggtt ctggttctgg ttctggttct      240

ggttctctta agacatcctt taaaatcgca ttcattcagg cccgctggca cgccgacatc      300

gttgacgaag cgcgcaaaag ctttgtcgcc gaactggccg caaagacggg tggcagcgtc      360

gaggtagaga tattcgacgt gccgggtgca tatgaaattc cccttcacgc caagacattg      420

gccagaaccg ggcgctatgc agccatcgtc ggtgcggcct tcgtgatcga cggcggcatc      480

tatcgtcatg atttcgtggc gacggccgtt atcaacggca tgatgcaggt gcagcttgaa      540

acggaagtgc cggtgctgag cgtcgtgctg acgccgcacc atttccatga aagcaaggag      600

catcacgact tcttccatgc tcatttcaag gtgaagggcg tggaagcggc ccatgccgcc      660

ttgcagatcg tgagcgagcg cagccgcatc gcgcttgtct ga                         702


<210>  7
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7

Met His Ala Asp Cys Ala Lys Gly Lys Ile Glu Phe Ser Lys Tyr Asn 
1               5                   10                  15      


Glu Asp Asp Thr Phe Thr Val Lys Val Asp Gly Lys Glu Tyr Trp Thr 
            20                  25                  30          


Ser Arg Trp Asn Leu Gln Pro Leu Leu Gln Ser Ala Gln Leu Thr Gly 
        35                  40                  45              


Met Thr Val Thr Ile Lys Ser Ser Thr Cys Glu Ser Gly Ser Gly Phe 
    50                  55                  60                  


Ala Glu Val Gln Phe Asn Asn Asp Gly Ser Gly Ser Gly Ser Gly Ser 
65                  70                  75                  80  


Gly Ser Gly Ser Gly Ser Gly Leu Lys Thr Ser Phe Lys Ile Ala Phe 
                85                  90                  95      


Ile Gln Ala Arg Trp His Ala Asp Ile Val Asp Glu Ala Arg Lys Ser 
            100                 105                 110         


Phe Val Ala Glu Leu Ala Ala Lys Thr Gly Gly Ser Val Glu Val Glu 
        115                 120                 125             


Ile Phe Asp Val Pro Gly Ala Tyr Glu Ile Pro Leu His Ala Lys Thr 
    130                 135                 140                 


Leu Ala Arg Thr Gly Arg Tyr Ala Ala Ile Val Gly Ala Ala Phe Val 
145                 150                 155                 160 


Ile Asp Gly Gly Ile Tyr Arg His Asp Phe Val Ala Thr Ala Val Ile 
                165                 170                 175     


Asn Gly Met Met Gln Val Gln Leu Glu Thr Glu Val Pro Val Leu Ser 
            180                 185                 190         


Val Val Leu Thr Pro His His Phe His Glu Ser Lys Glu His His Asp 
        195                 200                 205             


Phe Phe His Ala His Phe Lys Val Lys Gly Val Glu Ala Ala His Ala 
    210                 215                 220                 


Ala Leu Gln Ile Val Ser Glu Arg Ser Arg Ile Ala Leu Val 
225                 230                 235             


<210>  8
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8
atgcatgcgg attgtgctaa aggtaaaatt gagttttcca agtataatga ggatgacaca       60

tttacagtga aggttgacgg gaaagaatac tggaccagtc gctggaatct gcaaccgtta      120

ctgcaaagtg ctcagttgac aggaatgact gtcacaatca aatccagtac ctgtgaatca      180

ggctccggat ttgctgaagt gcagtttaat aatgacggtt ctggttctgg ttctggttct      240

ggttctggtt ctggttctgg tcttaagaca tcctttaaaa tcgcattcat tcaggcccgc      300

tggcacgccg acatcgttga cgaagcgcgc aaaagctttg tcgccgaact ggccgcaaag      360

acgggtggca gcgtcgaggt agagatattc gacgtgccgg gtgcatatga aattcccctt      420

cacgccaaga cattggccag aaccgggcgc tatgcagcca tcgtcggtgc ggccttcgtg      480

atcgacggcg gcatctatcg tcatgatttc gtggcgacgg ccgttatcaa cggcatgatg      540

caggtgcagc ttgaaacgga agtgccggtg ctgagcgtcg tgctgacgcc gcaccatttc      600

catgaaagca aggagcatca cgacttcttc catgctcatt tcaaggtgaa gggcgtggaa      660

gcggcccatg ccgccttgca gatcgtgagc gagcgcagcc gcatcgcgct tgtctga         717


<210>  9
<211>  158
<212>  PRT
<213>  Brucella abortus

<400>  9

Ala Ser Asn Gln Ser Cys Pro Asn Lys Thr Ser Phe Lys Ile Ala Phe 
1               5                   10                  15      


Ile Gln Ala Arg Trp His Ala Asp Ile Val Asp Glu Ala Arg Lys Ser 
            20                  25                  30          


Phe Val Ala Glu Leu Ala Ala Lys Thr Gly Gly Ser Val Glu Val Glu 
        35                  40                  45              


Ile Phe Asp Val Pro Gly Ala Tyr Glu Ile Pro Leu His Ala Lys Thr 
    50                  55                  60                  


Leu Ala Arg Thr Gly Arg Tyr Ala Ala Ile Val Gly Ala Ala Phe Val 
65                  70                  75                  80  


Ile Asp Gly Gly Ile Tyr Arg His Asp Phe Val Ala Thr Ala Val Ile 
                85                  90                  95      


Asn Gly Met Met Gln Val Gln Leu Glu Thr Glu Val Pro Val Leu Ser 
            100                 105                 110         


Val Val Leu Thr Pro His His Phe His Glu Ser Lys Glu His His Asp 
        115                 120                 125             


Phe Phe His Ala His Phe Lys Val Lys Gly Val Glu Ala Ala His Ala 
    130                 135                 140                 


Ala Leu Gln Ile Val Ser Glu Arg Ser Arg Ile Ala Leu Val 
145                 150                 155             


<210>  10
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  10
atcaacatgc atgcggattg tgctaaaggt                                        30


<210>  11
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  11
taaaatctta agaccagaac cagaaccgtc attattaaac tgcac                       45


<210>  12
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

Synthetic primer

<400>  12
gtttaagaat tcgaaggaga taccaccatg cat                                    33


<210>  13
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  13
cgtagcgcga acagactcga tcgtactgac cacctgt                                37


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  14
atcaacatgc atgcggattg tgctaaaggt                                        30


<210>  15
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  15
taaaatctta agagaaccag aaccagaacc agaaccagaa ccgtcattat taaactgcac       60


<210>  16
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  16
gtttaagaat tcgaaggaga taccaccatg cat                                    33


<210>  17
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  17
cgtagcgcga acagactcga tcgtactgac cacctgt                                37


<210>  18
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

Synthetic primer

<400>  18
atcaacatgc atgcggattg tgctaaaggt                                        30


<210>  19
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

Synthetic primer

<400>  19
taaaatctta agaccagaac cagaaccaga accagaacca gaaccagaac cagaaccgtc       60

attattaaac tgcac                                                        75


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

Synthetic primer

<400>  20
gtttaagaat tcgaaggaga taccaccatg cat                                    33


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

Synthetic primer

<400>  21
cgtagcgcga acagactcga tcgtactgac cacctgt                                37


