                         SEQUENCE LISTING

<110>  NOVO NORDISK A/S
 
<120>  METHODS FOR PRODUCING PEPTIDES USING ENGINEERED INTEINS

<130>  8672WO01

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  198
<212>  PRT
<213>  MYCOBACTERIUM XENOPI

<400>  1

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Arg Ser Ala Phe Ser Val 
            100                 105                 110         


Asp Cys Ala Gly Phe Ala Arg Gly Lys Pro Glu Phe Ala Pro Thr Thr 
        115                 120                 125             


Tyr Thr Val Gly Val Pro Gly Leu Val Arg Phe Leu Glu Ala His His 
    130                 135                 140                 


Arg Asp Pro Asp Ala Gln Ala Ile Ala Asp Glu Leu Thr Asp Gly Arg 
145                 150                 155                 160 


Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro 
                165                 170                 175     


Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn 
            180                 185                 190         


Gly Phe Val Ser His Asn 
        195             


<210>  2
<211>  36
<212>  PRT
<213>  HOMO SAPIENS

<400>  2

Tyr Pro Ile Lys Pro Glu Ala Pro Gly Glu Asp Ala Ser Pro Glu Glu 
1               5                   10                  15      


Leu Asn Arg Tyr Tyr Ala Ser Leu Arg His Tyr Leu Asn Leu Val Thr 
            20                  25                  30          


Arg Gln Arg Tyr 
        35      


<210>  3
<211>  37
<212>  PRT
<213>  HOMO SAPIENS

<400>  3

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  4
<211>  67
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  tag

<400>  4

Met Pro Lys Tyr Leu Gly Val Lys Val Gly Asp Gly Gln Ile Val Lys 
1               5                   10                  15      


Ala Gly Asn Ile Leu Val Arg Gln Arg Gly Thr Arg Phe Tyr Pro Gly 
            20                  25                  30          


Lys Asn Val Gly Ile Gly Arg Asp Phe Thr Leu Phe Ala Leu Lys Asp 
        35                  40                  45              


Gly Arg Val Lys Phe Glu Thr Lys Asn Asn Lys Lys Tyr Val Ser Val 
    50                  55                  60                  


Tyr Glu Glu 
65          


<210>  5
<211>  10
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  tag

<400>  5

Met Gly Ser Ser His His His His His His 
1               5                   10  


<210>  6
<211>  12
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  tag

<400>  6

Met Lys His Gln His Gln His Gln His Gln His Gln 
1               5                   10          


<210>  7
<211>  13
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  tag

<400>  7

Met Arg His Gln His Gln His Gln His Gln His Gln Lys 
1               5                   10              


<210>  8
<211>  8
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  protease site

<400>  8

Ser Ser Ser Glu Val Leu Phe Gln 
1               5               


<210>  9
<211>  7
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  protease site

<400>  9

Ser Ser Asp Asp Asp Asp Lys 
1               5           


<210>  10
<211>  33
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  hPYY variant

<400>  10

Gly Pro Glu Ala Pro Gly Glu Asp Ala Ser Pro Glu Glu Leu Asn Arg 
1               5                   10                  15      


Tyr Tyr Ala Ser Leu Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg 
            20                  25                  30          


Tyr 
    


<210>  11
<211>  34
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  hPYY variant

<400>  11

Ile Lys Pro Glu Ala Pro Gly Glu Asp Ala Ser Pro Glu Glu Leu Asn 
1               5                   10                  15      


Arg Tyr Tyr Ala Ser Leu Arg His Tyr Leu Asn Leu Val Thr Arg Gln 
            20                  25                  30          


Arg Tyr 
        


<210>  12
<211>  37
<212>  PRT
<213>  HOMO SAPIENS

<400>  12

Ala Cys Asp Thr Ala Thr Cys Val Thr His Arg Leu Ala Gly Leu Leu 
1               5                   10                  15      


Ser Arg Ser Gly Gly Val Val Lys Asn Asn Phe Val Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Ala Phe 
        35          


<210>  13
<211>  32
<212>  PRT
<213>  HOMO SAPIENS

<400>  13

Cys Gly Asn Leu Ser Thr Cys Met Leu Gly Thr Tyr Thr Gln Asp Phe 
1               5                   10                  15      


Asn Lys Phe His Thr Phe Pro Gln Thr Ala Ile Gly Val Gly Ala Pro 
            20                  25                  30          


<210>  14
<211>  36
<212>  PRT
<213>  HOMO SAPIENS

<400>  14

Ala Pro Leu Glu Pro Val Tyr Pro Gly Asp Asn Ala Thr Pro Glu Gln 
1               5                   10                  15      


Met Ala Gln Tyr Ala Ala Asp Leu Arg Arg Tyr Ile Asn Met Leu Thr 
            20                  25                  30          


Arg Pro Arg Tyr 
        35      


<210>  15
<211>  34
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  hPYY variant

<400>  15

Ile Arg Pro Glu Ala Pro Gly Glu Asp Ala Ser Pro Glu Glu Leu Gln 
1               5                   10                  15      


Arg Tyr Tyr Ala Ser Leu Arg His Tyr Leu Asn Lys Val Thr Arg Gln 
            20                  25                  30          


Arg Tyr 
        


<210>  16
<211>  36
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  hAmylin variant

<400>  16

Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asp Phe Leu Arg 
1               5                   10                  15      


His Ser Ser Pro Asn Phe Gly Ala Ile Pro Ser Ser Thr Asn Val Gly 
            20                  25                  30          


Ser Arg Thr Tyr 
        35      


<210>  17
<211>  198
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  17

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Arg Ser Ala Phe Ser Val 
            100                 105                 110         


Asp Cys Ala Gly Phe Ala Arg Gly Lys Pro Glu Phe Ala Pro Thr Thr 
        115                 120                 125             


Tyr Thr Val Gly Val Pro Gly Leu Val Arg Phe Leu Glu Ala His His 
    130                 135                 140                 


Arg Asp Pro Asp Ala Gln Ala Ile Ala Asp Glu Leu Thr Asp Gly Arg 
145                 150                 155                 160 


Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro 
                165                 170                 175     


Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn 
            180                 185                 190         


Gly Phe Val Ser His Ala 
        195             


<210>  18
<211>  198
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  18

Cys Ile Cys Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Arg Ser Ala Phe Ser Val 
            100                 105                 110         


Asp Cys Ala Gly Phe Ala Arg Gly Lys Pro Glu Phe Ala Pro Thr Thr 
        115                 120                 125             


Tyr Thr Val Gly Val Pro Gly Leu Val Arg Phe Leu Glu Ala His His 
    130                 135                 140                 


Arg Asp Pro Asp Ala Gln Ala Ile Ala Asp Glu Leu Thr Asp Gly Arg 
145                 150                 155                 160 


Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro 
                165                 170                 175     


Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn 
            180                 185                 190         


Gly Phe Val Ser His Ala 
        195             


<210>  19
<211>  152
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  19

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val 
        115                 120                 125             


Gln Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile 
    130                 135                 140                 


Thr Asn Gly Phe Val Ser His Ala 
145                 150         


<210>  20
<211>  152
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  20

Cys Ile Cys Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val 
        115                 120                 125             


Gln Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile 
    130                 135                 140                 


Thr Asn Gly Phe Val Ser His Ala 
145                 150         


<210>  21
<211>  152
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  21

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Arg Ser Ala Phe Ser Asp 
            100                 105                 110         


Gly Arg Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val 
        115                 120                 125             


Gln Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile 
    130                 135                 140                 


Thr Asn Gly Phe Val Ser His Ala 
145                 150         


<210>  22
<211>  144
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  22

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Phe Tyr Tyr Ala Lys Val 
            100                 105                 110         


Ala Ser Val Thr Asp Ala Gly Val Gln Pro Val Tyr Ser Leu Arg Val 
        115                 120                 125             


Asp Thr Ala Asp His Ala Phe Ile Thr Asn Gly Phe Val Ser His Ala 
    130                 135                 140                 


<210>  23
<211>  158
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  23

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Arg Ser Ala Phe Ser Leu 
            100                 105                 110         


Asp Arg His Gly Asn Asp Gly Arg Phe Tyr Tyr Ala Lys Val Ala Ser 
        115                 120                 125             


Val Thr Asp Ala Gly Val Gln Pro Val Tyr Ser Leu Arg Val Asp Thr 
    130                 135                 140                 


Ala Asp His Ala Phe Ile Thr Asn Gly Phe Val Ser His Ala 
145                 150                 155             


<210>  24
<211>  150
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  24

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Leu Asp Arg His Gln Asn 
            100                 105                 110         


Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro 
        115                 120                 125             


Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn 
    130                 135                 140                 


Gly Phe Val Ser His Ala 
145                 150 


<210>  25
<211>  151
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  25

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Arg Asp Val Glu Thr Gly 
            100                 105                 110         


Glu Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln 
        115                 120                 125             


Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr 
    130                 135                 140                 


Asn Gly Phe Val Ser His Ala 
145                 150     


<210>  26
<211>  151
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  26

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Ala Asp Asn Leu Ala Leu 
            100                 105                 110         


Ala Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln 
        115                 120                 125             


Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr 
    130                 135                 140                 


Asn Gly Phe Val Ser His Ala 
145                 150     


<210>  27
<211>  152
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  27

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Gly Arg Gly Ser Gly Arg 
            100                 105                 110         


Gly Ser Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val 
        115                 120                 125             


Gln Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile 
    130                 135                 140                 


Thr Asn Gly Phe Val Ser His Ala 
145                 150         


<210>  28
<211>  151
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  28

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gly Ser Gly Ser Gly Ser Gly 
            100                 105                 110         


Ser Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln 
        115                 120                 125             


Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr 
    130                 135                 140                 


Asn Gly Phe Val Ser His Ala 
145                 150     


<210>  29
<211>  150
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  29

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Gly Ser Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Phe Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro 
        115                 120                 125             


Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn 
    130                 135                 140                 


Gly Phe Val Ser His Ala 
145                 150 


<210>  30
<211>  149
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  30

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Gly Ser Gly Ser Gly Ser Gly Ser Phe 
            100                 105                 110         


Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro Val 
        115                 120                 125             


Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn Gly 
    130                 135                 140                 


Phe Val Ser His Ala 
145                 


<210>  31
<211>  151
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  31

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Tyr Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln 
        115                 120                 125             


Pro Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr 
    130                 135                 140                 


Asn Gly Phe Val Ser His Ala 
145                 150     


<210>  32
<211>  150
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  32

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Tyr Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro 
        115                 120                 125             


Val Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn 
    130                 135                 140                 


Gly Phe Val Ser His Ala 
145                 150 


<210>  33
<211>  149
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Intein variant

<400>  33

Cys Ile Thr Gly Asp Ala Leu Val Ala Leu Pro Glu Gly Glu Ser Val 
1               5                   10                  15      


Arg Ile Ala Asp Ile Val Pro Gly Ala Arg Pro Asn Ser Asp Asn Ala 
            20                  25                  30          


Ile Asp Leu Lys Val Leu Asp Arg His Gly Asn Pro Val Leu Ala Asp 
        35                  40                  45              


Arg Leu Phe His Ser Gly Glu His Pro Val Tyr Thr Val Arg Thr Val 
    50                  55                  60                  


Glu Gly Leu Arg Val Thr Gly Thr Ala Asn His Pro Leu Leu Cys Leu 
65                  70                  75                  80  


Val Asp Val Ala Gly Val Pro Thr Leu Leu Trp Lys Leu Ile Asp Glu 
                85                  90                  95      


Ile Lys Pro Gly Asp Tyr Ala Val Ile Gln Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Ala Lys Val Ala Ser Val Thr Asp Ala Gly Val Gln Pro Val 
        115                 120                 125             


Tyr Ser Leu Arg Val Asp Thr Ala Asp His Ala Phe Ile Thr Asn Gly 
    130                 135                 140                 


Phe Val Ser His Ala 
145                 


