PT628PCT.txt
               SEQUENCE LISTING
 
<110> University College Dublin National University of Ireland
 
<120> Therapies for cardiomyopathy
 
<130> PT628PCT
 
<160> 31
 
<170> BiSSAP 1.2
 
<210> 1
<211> 725
<212> DNA
<213> Homo sapiens
 
<220> 
<221> source
<222> 1..725
<223> /mol_type="unassigned DNA"
      /organism="Homo sapiens"
 
<220> 
<221> primer_bind
<222> 1..24
<223> /note="Forward Primer without XhoI"
 
<220> 
<221> protein_bind
<222> 97..113
<223> /function="Hypoxia response element (HRE)"
 
<220> 
<221> primer_bind
<222> 700..717
<223> /note="Reverse Primer without HindIII (sequence is reversed and       complemented to primer)"
 
<400> 1
gagatgcagt ttctctatgt tacctaggct ggtctaaaac tcctgggctc aagcgatcct      60
 
cccaccctgg cctcccaaag tgctgggatg acaggcgtga gccacgtggt gcttaaaaaa     120
 
ggcaacaaaa aaccccccac acactgggta tagaagtggc atgggcctct atacactgtg     180
 
agattcttgg tactagctac aaattctgtg tatactcaag attttctaga gtaggtgcaa     240
 
ttaccccgtt ttacagatga ggacacagag gctgagccgt agtgacccac ctaaggtcgt     300 atagccagca aatagatgga ggttggattg gaactgagga ctttactcaa gggctctcac     360
 
aaacccttgg gggcttctcg ctgctttatc cccatcacac ctgaaagaat gaatgaatga     420
 
atgcctcggg caccgtgccc acctcccagc aaaccgtgga gcttggacga gcccactgct     480
 
ccgcgtgggg ggggtgtgtg cccgccttgc gcatgcgtgt tccctgggca tggccggctc     540
 
cgttccatcc ttctgcacag ggtatcgcct ctctccgttt ggtacatccc ctcctccccc     600
 
acgcccggac tggggtggta gacgccgcct ccgctcatcg cccctcccca tcggtttccg     660
 
cgcgaaaagc cggggcgcct gcgctgccgc cgccgcgtct gctgaagcct ccgagatgcc     720
 
ggcgc                                                                 725
 
 
<210> 2
<211> 30
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..30
<223> /mol_type="unassigned DNA"
      /note="Forward Primer with XhoI"
      /organism="Artificial Sequence"
 
<220> 
<221> misc_feature
<222> 1..6
<223> /function="XhoI restriction site"
 
<400> 2 ctcgaggaga tgcagtttct ctatgttacc                                     30
 
 
<210> 3
<211> 24
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..24
<223> /mol_type="unassigned DNA"
      /note="DNAMT1 reverse primer with Hind iii"
      /organism="Artificial Sequence"
 
<220> 
<221> misc_feature
<222> 1..6
<223> /function="HindIII restriction site"
 
<400> 3 aagcttatct cggaggcttc agca                                           24
 
 
<210> 4
<211> 725
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..725
<223> /mol_type="unassigned DNA"
      /note="DNMT1 Mutant Site V$HIFF (97-113)"
      /organism="Artificial Sequence"
 
<220> 
<221> primer_bind
<222> 1..24
<223> /note="Forward Primer without XhoI"
 
<220> 
<221> protein_bind
<222> 97..113
<223> /function="Hypoxia response element (HRE) (HIF binding site)"
 
<220> 
<221> variation
<222> 104..105
<223> /note="Site-Directed Mutagenesis/Bases Changed"
 
<220> 
<221> primer_bind
<222> 700..717
<223> /note="Reverse Primer without HindIII (sequence is reversed and       complemented to primer)"
 
<400> 4 gagatgcagt ttctctatgt tacctaggct ggtctaaaac tcctgggctc aagcgatcct      60
 
cccaccctgg cctcccaaag tgctgggatg acaggcgtga gcctagtggt gcttaaaaaa     120
 
ggcaacaaaa aaccccccac acactgggta tagaagtggc atgggcctct atacactgtg     180 agattcttgg tactagctac aaattctgtg tatactcaag attttctaga gtaggtgcaa     240
 
ttaccccgtt ttacagatga ggacacagag gctgagccgt agtgacccac ctaaggtcgt     300
 
atagccagca aatagatgga ggttggattg gaactgagga ctttactcaa gggctctcac     360
 
aaacccttgg gggcttctcg ctgctttatc cccatcacac ctgaaagaat gaatgaatga     420
 
atgcctcggg caccgtgccc acctcccagc aaaccgtgga gcttggacga gcccactgct     480
 
ccgcgtgggg ggggtgtgtg cccgccttgc gcatgcgtgt tccctgggca tggccggctc     540
 
cgttccatcc ttctgcacag ggtatcgcct ctctccgttt ggtacatccc ctcctccccc     600
 
acgcccggac tggggtggta gacgccgcct ccgctcatcg cccctcccca tcggtttccg     660
 
cgcgaaaagc cggggcgcct gcgctgccgc cgccgcgtct gctgaagcct ccgagatgcc     720
 
ggcgc                                                                 725
 
 
<210> 5
<211> 38
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..38
<223> /mol_type="unassigned DNA"
      /note="Forward Primer"
      /organism="Artificial Sequence"
 
<400> 5 ggatgacagg cgtgagccta gtggtgctta aaaaaggc                             38
 
 
<210> 6
<211> 38
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..38
<223> /mol_type="unassigned DNA"
      /note="DNMT1 Mutant Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 6
gcctttttta agcaccacta ggctcacgcc tgtcatcc                             38
 
 
<210> 7
<211> 246
<212> DNA
<213> Homo sapiens
 
<220> 
<221> source
<222> 1..246
<223> /mol_type="unassigned DNA"
      /note="DNMT3b Promoter Wild-Type"
      /organism="Homo sapiens"
 
<220> 
<221> primer_bind
<222> 1..22
<223> /note="Forward Primer without XhoI"
 
<220> 
<221> protein_bind
<222> 108..124
<223> /function="Hypoxia response element (HRE) (HIF binding site)"
 
<220> 
<221> primer_bind
<222> 223..246
<223> /note="Reverse Primer without HindIII (sequence is reversed and       complemented to primer)"
 
<400> 7 gggccggggc tacaagggga gtcggcaccg ccccctcccc acccactccc gctgccccgt     60
 
ccggcccgcg ccgcttcctc gcagcagctg ctcccggctc cgcggccgca gcccgcgtgg    120
 
acgctccgag cgccccccga cggacgggac cggctccctg gcggtcgggc gagcgggcgg    180
 
caacgctgcc cggccggcag cgctggggtt aagtggccca agtaaaccta gctcggcgat    240
 
cggcgc                                                               246
 
 
<210> 8
<211> 28
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..28
<223> /mol_type="unassigned DNA"
      /note="DNMT3b Forward Primer with XhoI"
      /organism="Artificial Sequence"
 
<220> 
<221> misc_feature
<222> 1..6
<223> /function="XhoI restriction site"
 
<400> 8 ctcgaggggc cggggctaca aggggagt                                       28
 
 
<210> 9
<211> 30
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..30
<223> /mol_type="unassigned DNA"
      /note="Reverse Primer with HindIII"
      /organism="Artificial Sequence"
 
<220> 
<221> misc_feature
<222> 1..6
<223> /function="HindIII restriction site"
 
<400> 9 aagcttgcgc cgatcgccga gctaggttta                                     30
 
 
<210> 10
<211> 246
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..246
<223> /mol_type="unassigned DNA"
      /note="DNMT3b Mutant Site V$HIFF (108-124)"
      /organism="Artificial Sequence"
 
<220> 
<221> primer_bind
<222> 223..246
<223> /note="Reverse Primer without HindIII (sequence is reversed and       complemented to primer)"
 
<220> 
<221> primer_bind
<222> 1..22
<223> /note="Forward Primer without XhoI"
 
<220> 
<221> protein_bind
<222> 108..124
<223> /function="Hypoxia response element (HRE) (HIF binding site)"
 
<220> 
<221> variation
<222> 112,116
<223> /note="Site-Directed Mutagenesis/Bases Changed"
 
<400> 10 gggccggggc tacaagggga gtcggcaccg ccccctcccc acccactccc gctgccccgt     60
 
ccggcccgcg ccgcttcctc gcagcagctg ctcccggctc cgcggccgca gaccgagtgg    120
 
acgctccgag cgccccccga cggacgggac cggctccctg gcggtcgggc gagcgggcgg    180
 
caacgctgcc cggccggcag cgctggggtt aagtggccca agtaaaccta gctcggcgat    240
 
cggcgc                                                               246
 
 
<210> 11
<211> 35
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..35
<223> /mol_type="unassigned DNA"
      /note="DNAMT3b Mutant Forward Primer"
      /organism="Artificial Sequence"
 
<400> 11 gctccgcggc cgcagaccga gtggacgctc cgagc                                35
 
 
<210> 12
<211> 35
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..35
<223> /mol_type="unassigned DNA"
      /note="DNAMT3b Mutant Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 12 gctcggagcg tccactcggt ctgcggccgc ggagc                                35
 
 
<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Alpha Smooth Muscle Actin Forward Primer"
      /organism="Artificial Sequence"
 
<400> 13 cgttactact gctgagcgtg a                                              21
 
 
<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Alpha smooth muscle actin"
      /organism="Artificial Sequence"
 
<400> 14 aacgttcatt tccgatggtg                                                20
 
 
<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Collagen 1 ?1 Forward Primer"
      /organism="Artificial Sequence"
 
<400> 15 gaacgcgtgt catcccttgt                                                20
 
 
<210> 16
<211> 24
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..24
<223> /mol_type="unassigned DNA"
      /note="Collagen 1 ?1 Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 16 gaacgaggta gtctttcagc aaca                                           24
 
 
<210> 17
<211> 21
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Collagen 3 ?1 Forward Primer"
      /organism="Artificial Sequence"
 
<400> 17 aacacgcaag gctgtgagac t                                              21
 
 
<210> 18
<211> 24
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..24
<223> /mol_type="unassigned DNA"
      /note="Collagen 3 ?1 Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 18 gaacgaggta gtctttcagc aaca                                           24
 
 
<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Carbonic anhydrase IX"
      /organism="Artificial Sequence"
 
<400> 19 aggtcccagg actggacata                                                20
 
 
<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Carbonic anhydrase IX Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 20 gagggtgtgg agctgcttag                                                20
 
 
<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="DNA methyltransferase 1 Forward Primer"       /organism="Artificial Sequence"
 
<400> 21 tatccgagga gggctacctg                                                20
 
 
<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="DNA methyltransferase 1 Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 22 cacttcccgg ttgtaagcat                                                20
 
 
<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="DNA methyltransferase 3A"
      /organism="Artificial Sequence"
 
<400> 23 agcccaaggt caaggagatt                                                20
 
 
<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="DNA methyltransferase 3A Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 24 gttcttgcag ttttggcaca                                                20
 
 
<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="DNA methyltransferase 3B Forward Primer"
      /organism="Artificial Sequence"
 
<400> 25 tcaggatggg aaggagtttg                                                20
 
 
<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="DNA methyltransferase 3B Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 26 ctgcagagac ctcggagaac                                                20
 
 
<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Beta-2-microglobulin Forward Primer"
      /organism="Artificial Sequence"
 
<400> 27 aggctatcca gcgtactcca                                                20 <210> 28
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Beta-2-microglobulin Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 28 ccagtccttg ctgaaagaca                                                20
 
 
<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Myosin binding protein C Forward Primer"
      /organism="Artificial Sequence"
 
<400> 29 ctggagacct ggacctcaga                                                20
 
 
<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Myosin binding protein C Reverse Primer"
      /organism="Artificial Sequence"
 
<400> 30 ccggaaactg ctcttcttca                                                20 <210> 31
<211> 21
<212> RNA
<213> Artificial Sequence
 
<220> 
<221> source
<222> 1..21
<223> /mol_type="other RNA"
      /note="DNMT3B siRNA"
      /organism="Artificial Sequence"
 
<400> 31 agaugacgga ugccuagagu u                                              21
 
 
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