
                                SEQUENCE LISTING

<110> The Regents of the University of California
      Marriott, Gerard
      Hoepker, Alexander
      Yan, Yuling

<120> GENETICALLY-ENCODED SENSORS FOR IMAGING
  PROTEINS AND THEIR COMPLEXES
  

<130> LBNL.058WO

<150> 61/825434           
<151> 2013-05-20  

<160> 13

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 923
<212> PRT
<213> Avena sativa

<220> 
<223> NPH1-1

<400> 1
Met Ala Ser Lys Gly Ala Gly Gly Gly Gly Gly Gly His Glu Glu Pro
 1               5                  10                  15      
Gln Arg Pro Lys Gln Gln Leu Pro Arg Asp Ser Arg Gly Ser Leu Glu
            20                  25                  30          
Val Phe Asn Pro Ser Ser Ser Ser Ala Ala Val Glu Pro Pro Ser Ala
        35                  40                  45              
Phe Arg Pro Ala Ala Arg Ser Ala Ser Pro Phe Ile Glu Glu Ala Thr
    50                  55                  60                  
Gly Gly Ile Glu Asp Val Gly Lys Ala Thr Gln Arg Ala Ala Glu Trp
65                  70                  75                  80  
Gly Leu Val Leu Gln Thr Asn Glu Gln Thr Gly Arg Pro Gln Gly Val
                85                  90                  95      
Ser Ala Arg Ser Ser Gly Gly Gly Gly Ser Ala Arg Ser Ser Ser Asp
            100                 105                 110         
Asp Lys Ala Val Ala Gly Ala Ile Pro Arg Val Ser Glu Glu Leu Arg
        115                 120                 125             
Ala Ala Leu Ser Ala Phe Gln Gln Thr Phe Val Val Ser Asp Ala Ser
    130                 135                 140                 
Arg Pro Gly His Pro Ile Met Tyr Ala Ser Ala Gly Phe Phe Asn Met
145                 150                 155                 160 
Thr Gly Tyr Thr Ser Lys Glu Val Val Gly Arg Asn Cys Arg Phe Leu
                165                 170                 175     
Gln Gly Ser Gly Thr Asp Pro Ala Glu Ile Ala Lys Ile Arg Gln Ala
            180                 185                 190         
Leu Ala Asn Gly Ser Asn Tyr Cys Gly Arg Val Leu Asn Tyr Lys Lys
        195                 200                 205             
Asp Gly Thr Ala Phe Trp Asn Leu Leu Thr Ile Ala Pro Ile Lys Asp
    210                 215                 220                 
Glu Glu Gly Arg Val Leu Lys Phe Ile Gly Met Gln Val Glu Val Ser
225                 230                 235                 240 
Lys Tyr Thr Glu Gly Asn Lys Asp Thr Val Val Arg Pro Asn Gly Leu
                245                 250                 255     
Pro Glu Ser Leu Ile Lys Tyr Asp Ala Arg Gln Lys Asp Gln Ala Arg
            260                 265                 270         
Ser Ser Val Ser Glu Leu Leu Leu Ala Ile Lys Asn Pro Arg Ser Leu
        275                 280                 285             
Ser Glu Ser Thr Asn Ser Thr Phe Lys Arg Lys Ser Gln Glu Ser Val
    290                 295                 300                 
Gly Ala Leu Thr Gly Asp Arg Pro Gly Lys Arg Ser Ser Glu Ser Gly
305                 310                 315                 320 
Ser Arg Arg Asn Ser Lys Ser Gly Ala Arg Thr Ser Leu Gln Lys Ile
                325                 330                 335     
Ser Glu Val Pro Glu Arg Gly Ser Lys Ser Arg Lys Ser Gly Leu Tyr
            340                 345                 350         
Ser Leu Met Ser Leu Leu Gly Met Gly Pro Gly Asn Ile Glu Lys Asp
        355                 360                 365             
Met Leu Lys Pro Arg Asp Glu Asp Pro Leu Leu Asp Ser Asp Asp Glu
    370                 375                 380                 
Arg Pro Glu Ser Phe Asp Asp Glu Leu Arg Arg Lys Glu Met Arg Arg
385                 390                 395                 400 
Gly Ile Asp Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val
                405                 410                 415     
Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp
            420                 425                 430         
Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg
        435                 440                 445             
Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg
    450                 455                 460                 
Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu
465                 470                 475                 480 
Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu
                485                 490                 495     
Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val
            500                 505                 510         
Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly
        515                 520                 525             
Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys
    530                 535                 540                 
Glu Leu Pro Asp Ala Asn Leu Arg Pro Glu Asp Leu Trp Ala Asn His
545                 550                 555                 560 
Ser Lys Val Val Leu Pro Lys Pro His Met Lys Asp Ser Ala Ser Trp
                565                 570                 575     
Arg Ala Ile Gln Lys Val Leu Glu Gly Gly Glu Asn Ile Asp Leu Lys
            580                 585                 590         
His Phe Arg Pro Val Lys Pro Leu Gly Ser Gly Asp Thr Gly Ser Val
        595                 600                 605             
His Leu Val Glu Leu Leu Asn Thr Gly Glu Tyr Phe Ala Met Lys Ala
    610                 615                 620                 
Met Asp Lys Asn Val Met Leu Asn Arg Asn Lys Val His Arg Ala Asn
625                 630                 635                 640 
Ala Glu Arg Glu Ile Leu Asp Met Leu Asp His Pro Phe Leu Pro Thr
                645                 650                 655     
Leu Tyr Ala Ser Phe Gln Thr Lys Thr His Ile Cys Leu Ile Thr Asp
            660                 665                 670         
Tyr Tyr Pro Gly Gly Glu Leu Phe Leu Leu Leu Asp Arg Gln Pro Leu
        675                 680                 685             
Lys Val Leu Arg Glu Asp Ala Val Arg Phe Tyr Ala Ala Glu Val Val
    690                 695                 700                 
Ile Ala Leu Glu Tyr Leu His Cys Gln Gly Ile Ile Tyr Arg Asp Leu
705                 710                 715                 720 
Lys Pro Glu Asn Ile Leu Leu His Arg Asp Gly His Ile Ser Leu Thr
                725                 730                 735     
Asp Phe Asp Leu Ser Cys Leu Thr Ser Cys Arg Pro Gln Val Phe Leu
            740                 745                 750         
Pro Glu Glu Ala Asn Lys Lys Ser Arg Arg Lys Ser Arg Ser Ser Pro
        755                 760                 765             
Ile Phe Phe Ala Glu Pro Met Arg Ala Ser Asn Ser Phe Val Gly Thr
    770                 775                 780                 
Glu Glu Tyr Ile Ala Pro Glu Ile Ile Thr Gly Ala Gly His Thr Ser
785                 790                 795                 800 
Ala Val Asp Trp Trp Ala Leu Gly Ile Leu Leu Tyr Glu Met Leu Tyr
                805                 810                 815     
Gly Tyr Thr Pro Phe Arg Gly Lys Thr Arg Gln Arg Thr Phe Ala Asn
            820                 825                 830         
Ile Leu His Lys Asp Ile Arg Phe Pro Ala Ser Ile Ser Val Ser Leu
        835                 840                 845             
Pro Ala Arg Gln Leu Ile Tyr Arg Leu Leu His Arg Asp Pro Ser Asn
    850                 855                 860                 
Arg Leu Gly Ser Tyr Glu Gly Ser Asn Glu Ile Lys Glu His Pro Phe
865                 870                 875                 880 
Phe Arg Gly Ile Asn Trp Ala Leu Val Arg Gly Thr Ala Pro Pro Lys
                885                 890                 895     
Leu Asp Ala Pro Leu Phe Pro Asp Asp Thr Asp Lys Gly Met Gly Asp
            900                 905                 910         
Ala Ala Ala Ala Asp Thr His Thr Asp Met Phe
        915                 920             


<210> 2
<211> 3467
<212> DNA
<213> Avena sativa

<220> 
<221> misc_feature   
<222> 30, 3398
<223> n = a, c, t or g

<400> 2
aggtcgacac tagtggatcc aaagaattcn ggcacgagcc ccccaattcc cttcgtccca 60
cggccatgcc gcgcggtctt ccgagcgggc atatacgtac cctagctacg cgcgcagcaa 120
gcatctactg tgcagatcgc ctctcgcttc acgcgcaccg gcgcaaaggc tagccaagtg 180
ctcgctgcct ctgccccgtc tcgacctctc ttcctagcta gctccctcgt agagtccact 240
gcgcactgct cgcgagcaga taaagaaata tagtgggatg gagagctgag gtgcgtgcgg 300
ttttaggttc ttggatcacg cgccagcgag cacgccaaga tagaaggagg aggaaggaag 360
agtcgctgct actggcctgc tatttagctg aagcttcaca tcaatggctt ccaaaggtgc 420
aggaggcggc ggcggcggcc atgaggagcc tcagcggccg aagcagcagc tgccgcgcga 480
ctcccgcggc tcgctcgagg tcttcaaccc ctcttcctcc tccgccgccg tcgagccccc 540
ctccgcgttc cgccccgccg ccaggtccgc ctccccgttc atcgaggaag caacgggcgg 600
catcgaggat gtcggcaagg ccacgcagcg ggcggcggag tggggcctcg tgctccagac 660
caacgagcag acgggccggc cgcagggcgt gtccgcccga tcctccggag gcggcggctc 720
cgcccgcagc agctccgacg acaaggccgt cgccggcgcc atcccccggg tctcggagga 780
gctccgggcc gcgctctccg cgttccagca gaccttcgtc gtgtcggacg ccagccgacc 840
cggccaccca atcatgtacg ccagcgccgg cttcttcaac atgaccggct acacatccaa 900
ggaggtcgtc ggaaggaact gccgcttcct ccaaggctcc ggcaccgacc cggcggagat 960
tgccaagatc aggcaggctc tagcaaatgg atcaaactac tgcggccgtg tcctcaacta 1020
caagaaggac ggcaccgcat tctggaacct cttgaccatt gccccaatca aggatgaaga 1080
aggcagggtc ctcaagttca tagggatgca agtggaagta agtaaataca ctgaagggaa 1140
caaggatacg gttgttcgtc caaatggcct gccagagtca ctcatcaaat atgatgccag 1200
gcaaaaggat caggcccgta gctcagtgtc tgagcttctg ttggctatca agaatccacg 1260
atcattgtca gaatcaacta atagcacctt taaaagaaaa tcacaggaat cagtaggtgc 1320
attgacgggt gatcgtcctg gcaagagaag ctcagaaagt ggatctcgac gtaactcaaa 1380
atctggagca agaacctcac tgcaaaagat cagcgaagta cctgaaagag gcagtaaaag 1440
tagaaaatct ggtctgtatt cacttatgag tttacttggt atgggccctg gaaatataga 1500
aaaggacatg ctgaaaccaa gagatgaaga cccgctactt gacagtgatg atgaaagacc 1560
tgagagtttt gatgatgagc taaggcggaa agaaatgaga aggggtatag acttggctac 1620
tacacttgaa cgtattgaga agaactttgt cattactgac ccaaggttgc cagataatcc 1680
cattatattc gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt 1740
gggaagaaac tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat 1800
tagagatgcc atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag 1860
tggtaaaaag ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt 1920
ccagtacttt attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag 1980
agagggagtc atgctgatta agaaaactgc agaaaatatt gatgaggcgg caaaagaact 2040
tccagatgct aatttgagac cagaggattt gtgggctaac cactcaaaag tagttttgcc 2100
aaagccacat atgaaggatt ctgcatcatg gagagccatc caaaaagttc ttgagggtgg 2160
agaaaacatt gatttgaagc atttcaggcc tgtaaagcct ttgggatctg gtgacactgg 2220
aagcgtgcac ttggtggagt tattaaacac aggtgaatac tttgccatga aagctatgga 2280
taaaaacgtc atgcttaacc gcaataaggt tcatagagct aacgctgaac gagaaatcct 2340
tgatatgttg gatcacccat tccttccgac attatatgcg tcatttcaga caaagacaca 2400
tatatgtctc attacagact actaccctgg cggggagctc tttctgctcc tagatagaca 2460
acctctaaag gttctgcggg aagatgccgt caggttctat gctgctgaag ttgtcattgc 2520
acttgaatac ttgcattgcc aaggaataat ctaccgagac ttgaagccag agaatatctt 2580
acttcacagg gacgggcaca tctccttgac agactttgat ttgtcttgtc tgacatcttg 2640
cagaccgcag gtctttcttc cagaagaagc taataagaaa agtaggagga aaagcaggag 2700
ttcacccata ttttttgctg aacctatgcg agcatccaat tcatttgttg gtacagagga 2760
gtacattgca cctgagatca ttactggagc tggccataca agtgctgttg attggtgggc 2820
gctagggatc ctcctgtatg aaatgttgta tggttacaca cccttcagag gtaaaaccag 2880
gcagaggaca ttcgccaaca tcctacacaa ggacatcaga tttcccgcga gtatatcggt 2940
gagcctccca gcaaggcagc tgatatatag gctgctacac agggatcctt cgaataggct 3000
gggatcgtac gagggatcaa acgagataaa agaacaccct ttcttccgcg gcatcaactg 3060
ggctctcgtg cgtggcacgg ctcctccaaa gctggacgct ccactgttcc cggatgacac 3120
ggacaaggga atgggcgatg ctgctgctgc tgatactcac accgacatgt tctgaatgaa 3180
gaagctggct tggataaaaa cgcatctcga tcaagctcaa tcatgcatgc atgtttttgt 3240
ttgttgttga tagctgctgt ttactactgc ctaggattgt aggaataatt aagctagcct 3300
gggatcgctg gatgtaatgt aatgtattgt actgcgtgta tttggatcgt gcttaaataa 3360
taatcaagtt cagccaaaaa aaaaaaaaaa aaaactcnga gagtacttct agagcggccg 3420
cgggcccatc gattttccac ccgggtgggt accagtaatt acccaat               3467

<210> 3
<211> 109
<212> PRT
<213> Artificial Sequence

<220> 
<223> Avena sativa

<220> 
<223> NPH1-1 (AA 412-515) with N-terminal linker

<400> 3
Gly His Met Ala Ser Glu Lys Asn Phe Val Ile Cys Asp Pro Arg Leu
 1               5                  10                  15      
Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu Thr
            20                  25                  30          
Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Ala Arg Phe Leu Gln
        35                  40                  45              
Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala Ile
    50                  55                  60                  
Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys Ser
65                  70                  75                  80  
Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp Gln
                85                  90                  95      
Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp
            100                 105                 


<210> 4
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> Avena sativa

<220> 
<223> NPH1-1 (AA 412-490) with N-terminal linker

<400> 4
Met Ala Ser Glu Lys Asn Phe Val Ile Cys Asp Pro Arg Leu Pro Asp
 1               5                  10                  15      
Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu Thr Glu Tyr
            20                  25                  30          
Ser Arg Glu Glu Ile Leu Gly Arg Asn Ala Arg Phe Leu Gln Gly Pro
        35                  40                  45              
Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala Ile Asp Asn
    50                  55                  60                  
Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys Ser Gly Lys
65                  70                  75                  80  
Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp Gln Lys Gly
                85                  90                  95      
Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp
            100                 105         


<210> 5

<400> 5
 000

<210> 6
<211> 186
<212> PRT
<213> Photobacterium leiognathi

<220> 
<223> Lumazine protein (LUMP), gene: lumP

<400> 6
Met Phe Arg Gly Ile Val Gln Gly Arg Gly Val Ile Arg Ser Ile Ser
 1               5                  10                  15      
Lys Ser Glu Asp Ser Gln Arg His Gly Ile Ala Phe Pro Glu Gly Met
            20                  25                  30          
Phe Gln Leu Val Asp Val Asp Thr Val Met Leu Val Asn Gly Cys Ser
        35                  40                  45              
Leu Thr Val Val Arg Ile Leu Gly Asp Met Val Tyr Phe Asp Ile Asp
    50                  55                  60                  
Gln Ala Leu Gly Thr Thr Thr Phe Asp Gly Leu Lys Glu Gly Asp Gln
65                  70                  75                  80  
Val Asn Leu Glu Ile His Pro Lys Phe Gly Glu Val Val Gly Arg Gly
                85                  90                  95      
Gly Leu Thr Gly Asn Ile Lys Gly Thr Ala Leu Val Ala Ala Ile Glu
            100                 105                 110         
Glu Asn Asp Ala Gly Phe Ser Val Leu Ile Asp Ile Pro Lys Gly Leu
        115                 120                 125             
Ala Glu Asn Leu Thr Val Lys Asp Asp Ile Gly Ile Asp Gly Ile Ser
    130                 135                 140                 
Leu Pro Ile Thr Asp Met Ser Asp Ser Ile Ile Thr Leu Asn Tyr Ser
145                 150                 155                 160 
Arg Asp Leu Leu Ala Ser Thr Asn Ile Ala Ser Leu Ala Lys Asp Val
                165                 170                 175     
Lys Val Asn Val Glu Ile Leu Asn Glu Trp
            180                 185     


<210> 7
<211> 1929
<212> DNA
<213> Photobacterium leiognathi

<220> 
<223> Lumazine protein (LUMP), gene: lumP

<400> 7
ctgcagcagt taatcactac cattcattta aaatttcaac gttcactttc acatcttttg 60
ctaacgatgc aatgttagta ctcgctaaca aatcacgcga gtaattcaat gtaataatgc 120
tgtctgacat atcagtgata ggcaaagaga tcccatcaat tccaatatca tctttcactg 180
ttaagttttc agctaaccct ttagggatat caatcagtac agaaaaacct gcgtcattct 240
cttctattgc tgcgaccagc gcagtgcctt taatatttcc tgttaatcca ccacggcaaa 300
ccacttcacc aaatttaggg tggatctcta ggtttacctg atcgccttct tttaatccat 360
caaatgttgt cgtacccaat gcttggtcga tatcaaagta caccatatcg cctaaaatac 420
ggacaactgt caatgaacaa ccatttacta acattacagt atctacatca acaagttgaa 480
acataccctc agggaatgca atcccatgtc gctgactatc ttcacttttt gaaatggatc 540
gaataacacc acgaccttga acaatacctc taaacattac tgtctccttt tttagactct 600
atatgatatt ttcagtaaaa atacaactag cacatttatg agattctagt aattgaagaa 660
agaaatacaa aaagcggaca acatttttct attaccatca caaatttttt aattaaacaa 720
tattaaaatc aatcttgtat taacaatgaa atatcaataa tattttaatg ggcaattgaa 780
acattattca ttttacattc caattgaacg ctatataaac aattccagcc aatacccatc 840
aaaaatcccc ttaccccaat aaaaaataaa taaaaacaaa taaaaatcaa caacttaaca 900
aacaaaacca ccagcagata cttgctaaaa tccgtatata aaaggttgag taatatttac 960
ctaataaaaa taaaatctat tattaaaact taaaaaatac aaaacaaaat actgtttaat 1020
taaataaaaa acaccggagt aaatataaaa taaaaatata aaataaagtt caaaatataa 1080
ccaaaaagct taaatttaca tttataaaaa atcatagaaa taataaacac aataaattcc 1140
gcactgcatt ctatattttt tagaacgatt tttaagcaca tataagtgta aaaaaaactg 1200
ggcagagtaa atataaaaac catgaattaa aaataatatt gtcaacaatg tgagccaaat 1260
ctcaaaacgt aacaaatgca aaacaattta cttacactaa aaaaaatatt ctcctagcct 1320
aatttcattc cctatgcagc atgcgactat gcaattaggg tatataaaac ttacaatatg 1380
ctaatggaga ttgcatgatt aaaaagatcc cactgattat tggaggcgaa gttcaagaca 1440
cgtcagaaca tgatgtccgt gaacttacgc ttaacaataa caccgtcaat gtacctatca 1500
ttacggacaa agatgctgaa tctatcacct cactaaaaat agaaaataag ttaaatatca 1560
accagatagt taacttcttg tatactgtag ggcaaaaatg gaagagtgag aactacagcc 1620
gtcgcctcac ttatattcgt gatctagtaa aattcatggg ctactcccct gagatggcaa 1680
aactagaagc gaactggatt tcgatgattc tatgtagcaa aagtgcgcta tatgacattg 1740
ttgaaaatga tctcagctct cgtcatattg ttgatgaatg gctcccccaa ggtgattgct 1800
atgttaaagc gctaccaaaa ggtaaatcta tccatttatt agcgggtaac gttccgctat 1860
caggtgtgac atcgatcctg cgtgcaattt taactaaaaa tgaatgtatc attaaaacat 1920
catctgcag                                                         1929

<210> 8
<211> 104
<212> PRT
<213> Photobacterium leiognathi

<220> 
<223> Truncated Lumazine protein (LUMP, AA 1-102), gene:
      lumP

<400> 8
Gly His Met Phe Arg Gly Ile Val Gln Gly Arg Gly Val Ile Arg Ser
 1               5                  10                  15      
Ile Ser Lys Ser Glu Asp Ser Gln Arg His Gly Ile Ala Phe Pro Glu
            20                  25                  30          
Gly Met Phe Gln Leu Val Asp Val Asp Thr Val Met Leu Val Asn Gly
        35                  40                  45              
Cys Ser Leu Thr Val Val Arg Ile Leu Gly Asp Met Val Tyr Phe Asp
    50                  55                  60                  
Ile Asp Gln Ala Leu Gly Thr Thr Thr Phe Asp Gly Leu Lys Glu Gly
65                  70                  75                  80  
Asp Gln Val Asn Leu Glu Ile His Pro Lys Phe Gly Glu Val Val Gly
                85                  90                  95      
Arg Gly Gly Leu Thr Gly Asn Ile
            100                 


<210> 9
<211> 188
<212> PRT
<213> Photobacterium leiognathi

<220> 
<223> Lumazine protein (LUMP)

<400> 9
Gly His Met Phe Arg Gly Ile Val Gln Gly Arg Gly Val Ile Arg Ser
 1               5                  10                  15      
Ile Ser Lys Ser Glu Asp Ser Gln Arg His Gly Ile Ala Phe Pro Glu
            20                  25                  30          
Gly Met Phe Gln Leu Val Asp Val Asp Thr Val Met Leu Val Asn Gly
        35                  40                  45              
Cys Ser Leu Thr Val Val Arg Ile Leu Gly Asp Met Val Tyr Phe Asp
    50                  55                  60                  
Ile Asp Gln Ala Leu Gly Thr Thr Thr Phe Asp Gly Leu Lys Glu Gly
65                  70                  75                  80  
Asp Gln Val Asn Leu Glu Ile His Pro Lys Phe Gly Glu Val Val Gly
                85                  90                  95      
Arg Gly Gly Leu Thr Gly Asn Ile Lys Gly Thr Ala Leu Val Ala Ala
            100                 105                 110         
Ile Glu Glu Asn Asp Ala Gly Phe Ser Val Leu Ile Asp Ile Pro Lys
        115                 120                 125             
Gly Leu Ala Glu Asn Leu Thr Val Lys Asp Asp Ile Gly Ile Asp Gly
    130                 135                 140                 
Ile Ser Leu Pro Ile Thr Asp Met Ser Asp Ser Ile Ile Thr Leu Asn
145                 150                 155                 160 
Tyr Ser Arg Asp Leu Leu Ala Ser Thr Asn Ile Ala Ser Leu Ala Lys
                165                 170                 175     
Asp Val Lys Val Asn Val Glu Ile Leu Asn Glu Trp
            180                 185             


<210> 10
<211> 97
<212> PRT
<213> Artificial Sequence

<220> 
<223> Aliivibrio fischeri (Vibrio fischeri)
      

<220> 
<223> Truncated N-terminal domain of RS

<400> 10
Met Phe Thr Gly Ile Val Gln Gly Thr Ala Lys Leu Val Ser Ile Asp
 1               5                  10                  15      
Glu Lys Pro Asn Phe Arg Thr His Val Val Glu Leu Pro Asp His Met
            20                  25                  30          
Leu Asp Gly Leu Glu Thr Gly Ala Ser Val Ala His Asn Gly Cys Cys
        35                  40                  45              
Leu Thr Val Thr Glu Ile Asn Gly Asn His Val Ser Phe Asp Leu Met
    50                  55                  60                  
Lys Glu Thr Leu Arg Ile Thr Asn Leu Gly Asp Leu Lys Val Gly Asp
65                  70                  75                  80  
Trp Val Asn Val Glu Arg Ala Ala Lys Phe Ser Asp Glu Ile Gly Gly
                85                  90                  95      
His
    


<210> 11
<211> 122
<212> PRT
<213> Artificial Sequence

<220> 
<223> Aliivibrio fischeri (Vibrio fischeri)

<220> 
<223> Truncated N-terminal domain of RS with His-tag and
      TEV protease site at N-terminus

<400> 11
Met Gly Ser Ser His His His His His His Asp Tyr Asp Ile Pro Thr
 1               5                  10                  15      
Thr Glu Asn Leu Tyr Phe Gln Gly His Met Phe Thr Gly Ile Val Gln
            20                  25                  30          
Gly Thr Ala Lys Leu Val Ser Ile Asp Glu Lys Pro Asn Phe Arg Thr
        35                  40                  45              
His Val Val Glu Leu Pro Asp His Met Leu Asp Gly Leu Glu Thr Gly
    50                  55                  60                  
Ala Ser Val Ala His Asn Gly Cys Cys Leu Thr Val Thr Glu Ile Asn
65                  70                  75                  80  
Gly Asn His Val Ser Phe Asp Leu Met Lys Glu Thr Leu Arg Ile Thr
                85                  90                  95      
Asp Leu Gly Asp Leu Lys Val Gly Asp Trp Val Ala Val Glu Arg Ala
            100                 105                 110         
Ala Lys Phe Ser Asp Glu Ile Gly Gly His
        115                 120         


<210> 12
<211> 194
<212> PRT
<213> Vibrio fisherei

<220> 
<223> Y1


<400> 12
Met Phe Lys Gly Ile Val Glu Gly Ile Gly Ile Ile Glu Lys Ile Asp
 1               5                  10                  15      
Ile Tyr Thr Asp Leu Asp Lys Tyr Ala Ile Arg Phe Pro Glu Asn Met
            20                  25                  30          
Leu Asn Gly Ile Lys Lys Glu Ser Ser Ile Met Phe Asn Gly Cys Phe
        35                  40                  45              
Leu Thr Val Thr Ser Val Asn Ser Asn Ile Val Trp Phe Asp Ile Phe
    50                  55                  60                  
Glu Lys Glu Ala Arg Lys Leu Asp Thr Phe Arg Glu Tyr Lys Val Gly
65                  70                  75                  80  
Asp Arg Val Asn Leu Gly Thr Phe Pro Lys Phe Gly Ala Ala Ser Gly
                85                  90                  95      
Gly His Ile Leu Ser Ala Arg Ile Ser Cys Val Ala Ser Ile Ile Glu
            100                 105                 110         
Ile Ile Glu Asn Glu Asp Tyr Gln Gln Met Trp Ile Gln Ile Pro Glu
        115                 120                 125             
Asn Phe Thr Glu Phe Leu Ile Asp Lys Asp Tyr Ile Ala Val Asp Gly
    130                 135                 140                 
Ile Ser Leu Thr Ile Asp Thr Ile Lys Asn Asn Gln Phe Phe Ile Ser
145                 150                 155                 160 
Leu Pro Leu Lys Ile Ala Gln Asn Thr Asn Met Lys Trp Arg Lys Lys
                165                 170                 175     
Gly Asp Lys Val Asn Val Glu Leu Ser Asn Lys Ile Asn Ala Asn Gln
            180                 185                 190         
Cys Trp
        


<210> 13
<211> 771
<212> DNA
<213> Vibrio fisherei

<220> 
<223> Y1

<400> 13
aatattttta ttaattcatt agaaaaatga gaggaaggat tattatgttt aaaggtatag 60
tagaaggtat aggaatcatt gaaaaaattg atatatatac tgacctagat aagtatgcaa 120
ttcgatttcc tgaaaatatg ttgaatggaa ttaaaaagga gtcgtcaata atgtttaacg 180
gatgcttctt aacggtaact agcgtgaatt caaacattgt ctggtttgat atatttgaaa 240
aagaagcacg taagcttgat acttttcggg aatataaggt aggtgaccga gtaaatttag 300
gaacattccc aaaatttggc gctgcatctg gtgggcatat attatcagca aggatttcat 360
gtgtagcaag tattattgaa ataatagaaa atgaggatta tcaacaaatg tggattcaaa 420
ttcctgaaaa ttttacagag tttcttattg ataaagacta tattgctgtg gatggtatta 480
gcttaactat tgacactata aaaaacaacc aatttttcat tagtttaccc ttaaaaatag 540
cacaaaatac aaatatgaaa tggcgaaaaa aaggtgataa ggtaaatgtt gagttatcaa 600
acaaaattaa tgctaaccag tgttggtaat ttactgagga tagtaaaaat gaactgttta 660
aaataatatt taaattttta tttataatac agagtcagtt gttgtaaata gtctgagtgg 720
taaataagtt ctaccattaa ttaaatatta tccatattaa ataaaggatc t          771
