                         LISTADO DE SECUENCIAS

<110>  Abengoa Bioenerga Nuevas Tecnologas
 
<120>  "EXPRESIN DE ENZIMAS BETA-XILOSIDASAS RECOMBINANTES"

<130>  PCT1861.35

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1047
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Secuencia de cido nucleico codificante para el polipptido de SEQ ID NO: 2


<220>
<221>  sig_peptide
<222>  (1)..(60)

<400>  1
atgcagctca ggtttctgtc ttcagcattg ctgttgtctc tgaccagcaa atgcgctgcg       60

caaaacacta atgacattcc tccgctgatc accgacctct ggtccgcgga cccctcagct      120

catgttttcg aaggcaagct ctgggtttac ccatctcacg acatcgaagc caatgttgtc      180

aacggcacag gaggcgctca atacgccatg agggattacc atacctactc catgaagagc      240

atctatggta aagatcccgt tgtcgaccac ggcgtcgctc tctcagtcga tgacgttccc      300

tgggcgaagc agcaaatgtg ggctcctgac gcagctcata agaacggcaa atattatctg      360

tacttccccg ccaaggacaa ggatgagatc ttcagaattg gagttgctgt ctccaacaag      420

cccagcggtc ctttcaaggc tgacaagagc tggattcctg gcacgtacag tatcgaccct      480

gctagctacg tcgacaatga taaagaggcc tacctcatct ggggcggtat ctggggcggc      540

cagctccaag cctggcagga taaaaagaac tttaacgagt cgtggatcgg agacaaggct      600

gctcctaacg gcaccaatgc cctatcccct cagatcgcca agctaagcaa ggacatgcac      660

aagatcaccg aaacaccccg cgatctcgtc attctcgccc ccgagacagg caagcctctt      720

caggctgagg acaacaagcg acgattcttc gagggccctt ggattcacaa gcgcggcaag      780

ctgtactacc tcatgtactc caccggtgat acccacttcc ttgtctacgc tacttccaag      840

aacatctacg gtccttatac ctaccagggc aagattcttg atcctgttga tgggtggact      900

actcatggaa gtattgttga gtataaggga cagtggtggc ttttctttgc tgatgcgcat      960

acgtctggta aggattacct tcgacaggtg aaggcgagga agatctggta tgacaagaac     1020

ggcaagatct tgcttcaccg tccttag                                         1047


<210>  2
<211>  348
<212>  PRT
<213>  Fusarium oxysporum


<220>
<221>  SIGNAL
<222>  (1)..(20)

<400>  2

Met Gln Leu Arg Phe Leu Ser Ser Ala Leu Leu Leu Ser Leu Thr Ser 
1               5                   10                  15      


Lys Cys Ala Ala Gln Asn Thr Asn Asp Ile Pro Pro Leu Ile Thr Asp 
            20                  25                  30          


Leu Trp Ser Ala Asp Pro Ser Ala His Val Phe Glu Gly Lys Leu Trp 
        35                  40                  45              


Val Tyr Pro Ser His Asp Ile Glu Ala Asn Val Val Asn Gly Thr Gly 
    50                  55                  60                  


Gly Ala Gln Tyr Ala Met Arg Asp Tyr His Thr Tyr Ser Met Lys Ser 
65                  70                  75                  80  


Ile Tyr Gly Lys Asp Pro Val Val Asp His Gly Val Ala Leu Ser Val 
                85                  90                  95      


Asp Asp Val Pro Trp Ala Lys Gln Gln Met Trp Ala Pro Asp Ala Ala 
            100                 105                 110         


His Lys Asn Gly Lys Tyr Tyr Leu Tyr Phe Pro Ala Lys Asp Lys Asp 
        115                 120                 125             


Glu Ile Phe Arg Ile Gly Val Ala Val Ser Asn Lys Pro Ser Gly Pro 
    130                 135                 140                 


Phe Lys Ala Asp Lys Ser Trp Ile Pro Gly Thr Tyr Ser Ile Asp Pro 
145                 150                 155                 160 


Ala Ser Tyr Val Asp Asn Asp Lys Glu Ala Tyr Leu Ile Trp Gly Gly 
                165                 170                 175     


Ile Trp Gly Gly Gln Leu Gln Ala Trp Gln Asp Lys Lys Asn Phe Asn 
            180                 185                 190         


Glu Ser Trp Ile Gly Asp Lys Ala Ala Pro Asn Gly Thr Asn Ala Leu 
        195                 200                 205             


Ser Pro Gln Ile Ala Lys Leu Ser Lys Asp Met His Lys Ile Thr Glu 
    210                 215                 220                 


Thr Pro Arg Asp Leu Val Ile Leu Ala Pro Glu Thr Gly Lys Pro Leu 
225                 230                 235                 240 


Gln Ala Glu Asp Asn Lys Arg Arg Phe Phe Glu Gly Pro Trp Ile His 
                245                 250                 255     


Lys Arg Gly Lys Leu Tyr Tyr Leu Met Tyr Ser Thr Gly Asp Thr His 
            260                 265                 270         


Phe Leu Val Tyr Ala Thr Ser Lys Asn Ile Tyr Gly Pro Tyr Thr Tyr 
        275                 280                 285             


Gln Gly Lys Ile Leu Asp Pro Val Asp Gly Trp Thr Thr His Gly Ser 
    290                 295                 300                 


Ile Val Glu Tyr Lys Gly Gln Trp Trp Leu Phe Phe Ala Asp Ala His 
305                 310                 315                 320 


Thr Ser Gly Lys Asp Tyr Leu Arg Gln Val Lys Ala Arg Lys Ile Trp 
                325                 330                 335     


Tyr Asp Lys Asn Gly Lys Ile Leu Leu His Arg Pro 
            340                 345             


<210>  3
<211>  328
<212>  PRT
<213>  Fusarium oxysporum

<400>  3

Gln Asn Thr Asn Asp Ile Pro Pro Leu Ile Thr Asp Leu Trp Ser Ala 
1               5                   10                  15      


Asp Pro Ser Ala His Val Phe Glu Gly Lys Leu Trp Val Tyr Pro Ser 
            20                  25                  30          


His Asp Ile Glu Ala Asn Val Val Asn Gly Thr Gly Gly Ala Gln Tyr 
        35                  40                  45              


Ala Met Arg Asp Tyr His Thr Tyr Ser Met Lys Ser Ile Tyr Gly Lys 
    50                  55                  60                  


Asp Pro Val Val Asp His Gly Val Ala Leu Ser Val Asp Asp Val Pro 
65                  70                  75                  80  


Trp Ala Lys Gln Gln Met Trp Ala Pro Asp Ala Ala His Lys Asn Gly 
                85                  90                  95      


Lys Tyr Tyr Leu Tyr Phe Pro Ala Lys Asp Lys Asp Glu Ile Phe Arg 
            100                 105                 110         


Ile Gly Val Ala Val Ser Asn Lys Pro Ser Gly Pro Phe Lys Ala Asp 
        115                 120                 125             


Lys Ser Trp Ile Pro Gly Thr Tyr Ser Ile Asp Pro Ala Ser Tyr Val 
    130                 135                 140                 


Asp Asn Asp Lys Glu Ala Tyr Leu Ile Trp Gly Gly Ile Trp Gly Gly 
145                 150                 155                 160 


Gln Leu Gln Ala Trp Gln Asp Lys Lys Asn Phe Asn Glu Ser Trp Ile 
                165                 170                 175     


Gly Asp Lys Ala Ala Pro Asn Gly Thr Asn Ala Leu Ser Pro Gln Ile 
            180                 185                 190         


Ala Lys Leu Ser Lys Asp Met His Lys Ile Thr Glu Thr Pro Arg Asp 
        195                 200                 205             


Leu Val Ile Leu Ala Pro Glu Thr Gly Lys Pro Leu Gln Ala Glu Asp 
    210                 215                 220                 


Asn Lys Arg Arg Phe Phe Glu Gly Pro Trp Ile His Lys Arg Gly Lys 
225                 230                 235                 240 


Leu Tyr Tyr Leu Met Tyr Ser Thr Gly Asp Thr His Phe Leu Val Tyr 
                245                 250                 255     


Ala Thr Ser Lys Asn Ile Tyr Gly Pro Tyr Thr Tyr Gln Gly Lys Ile 
            260                 265                 270         


Leu Asp Pro Val Asp Gly Trp Thr Thr His Gly Ser Ile Val Glu Tyr 
        275                 280                 285             


Lys Gly Gln Trp Trp Leu Phe Phe Ala Asp Ala His Thr Ser Gly Lys 
    290                 295                 300                 


Asp Tyr Leu Arg Gln Val Lys Ala Arg Lys Ile Trp Tyr Asp Lys Asn 
305                 310                 315                 320 


Gly Lys Ile Leu Leu His Arg Pro 
                325             


<210>  4
<211>  346
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  Preprotena Beta-xilosidasa que comprende la SEQ ID NO: 3 y el pptido
       seal del gen de la glucoamilasa de Aspergillus niger


<220>
<221>  SIGNAL
<222>  (1)..(18)

<400>  4

Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Ser Gly 
1               5                   10                  15      


Leu Ala Gln Asn Thr Asn Asp Ile Pro Pro Leu Ile Thr Asp Leu Trp 
            20                  25                  30          


Ser Ala Asp Pro Ser Ala His Val Phe Glu Gly Lys Leu Trp Val Tyr 
        35                  40                  45              


Pro Ser His Asp Ile Glu Ala Asn Val Val Asn Gly Thr Gly Gly Ala 
    50                  55                  60                  


Gln Tyr Ala Met Arg Asp Tyr His Thr Tyr Ser Met Lys Ser Ile Tyr 
65                  70                  75                  80  


Gly Lys Asp Pro Val Val Asp His Gly Val Ala Leu Ser Val Asp Asp 
                85                  90                  95      


Val Pro Trp Ala Lys Gln Gln Met Trp Ala Pro Asp Ala Ala His Lys 
            100                 105                 110         


Asn Gly Lys Tyr Tyr Leu Tyr Phe Pro Ala Lys Asp Lys Asp Glu Ile 
        115                 120                 125             


Phe Arg Ile Gly Val Ala Val Ser Asn Lys Pro Ser Gly Pro Phe Lys 
    130                 135                 140                 


Ala Asp Lys Ser Trp Ile Pro Gly Thr Tyr Ser Ile Asp Pro Ala Ser 
145                 150                 155                 160 


Tyr Val Asp Asn Asp Lys Glu Ala Tyr Leu Ile Trp Gly Gly Ile Trp 
                165                 170                 175     


Gly Gly Gln Leu Gln Ala Trp Gln Asp Lys Lys Asn Phe Asn Glu Ser 
            180                 185                 190         


Trp Ile Gly Asp Lys Ala Ala Pro Asn Gly Thr Asn Ala Leu Ser Pro 
        195                 200                 205             


Gln Ile Ala Lys Leu Ser Lys Asp Met His Lys Ile Thr Glu Thr Pro 
    210                 215                 220                 


Arg Asp Leu Val Ile Leu Ala Pro Glu Thr Gly Lys Pro Leu Gln Ala 
225                 230                 235                 240 


Glu Asp Asn Lys Arg Arg Phe Phe Glu Gly Pro Trp Ile His Lys Arg 
                245                 250                 255     


Gly Lys Leu Tyr Tyr Leu Met Tyr Ser Thr Gly Asp Thr His Phe Leu 
            260                 265                 270         


Val Tyr Ala Thr Ser Lys Asn Ile Tyr Gly Pro Tyr Thr Tyr Gln Gly 
        275                 280                 285             


Lys Ile Leu Asp Pro Val Asp Gly Trp Thr Thr His Gly Ser Ile Val 
    290                 295                 300                 


Glu Tyr Lys Gly Gln Trp Trp Leu Phe Phe Ala Asp Ala His Thr Ser 
305                 310                 315                 320 


Gly Lys Asp Tyr Leu Arg Gln Val Lys Ala Arg Lys Ile Trp Tyr Asp 
                325                 330                 335     


Lys Asn Gly Lys Ile Leu Leu His Arg Pro 
            340                 345     


<210>  5
<211>  1041
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Secuencia de cido nucleico que codifica para el polipptido de SEQ ID NO: 4


<220>
<221>  sig_peptide
<222>  (1)..(54)

<400>  5
atgtcgttcc gatctcttct cgccctgagc ggccttgtct gctcggggtt ggcacaaaac       60

actaatgaca ttcctccgct gatcaccgac ctctggtccg cggacccctc agctcatgtt      120

ttcgaaggca agctctgggt ttacccatct cacgacatcg aagccaatgt tgtcaacggc      180

acaggaggcg ctcaatacgc catgagggat taccatacct actccatgaa gagcatctat      240

ggtaaagatc ccgttgtcga ccacggcgtc gctctctcag tcgatgacgt tccctgggcg      300

aagcagcaaa tgtgggctcc tgacgcagct cataagaacg gcaaatatta tctgtacttc      360

cccgccaagg acaaggatga gatcttcaga attggagttg ctgtctccaa caagcccagc      420

ggtcctttca aggctgacaa gagctggatt cctggcacgt acagtatcga ccctgctagc      480

tacgtcgaca atgataaaga ggcctacctc atctggggcg gtatctgggg cggccagctc      540

caagcctggc aggataaaaa gaactttaac gagtcgtgga tcggagacaa ggctgctcct      600

aacggcacca atgccctatc ccctcagatc gccaagctaa gcaaggacat gcacaagatc      660

accgaaacac cccgcgatct cgtcattctc gcccccgaga caggcaagcc tcttcaggct      720

gaggacaaca agcgacgatt cttcgagggc ccttggattc acaagcgcgg caagctgtac      780

tacctcatgt actccaccgg tgatacccac ttccttgtct acgctacttc caagaacatc      840

tacggtcctt atacctacca gggcaagatt cttgatcctg ttgatgggtg gactactcat      900

ggaagtattg ttgagtataa gggacagtgg tggcttttct ttgctgatgc gcatacgtct      960

ggtaaggatt accttcgaca ggtgaaggcg aggaagatct ggtatgacaa gaacggcaag     1020

atcttgcttc accgtcctta g                                               1041


<210>  6
<211>  987
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Secuencia de cido nucleico que codifica para el polipptido de SEQ ID NO: 3

<400>  6
caaaacacta atgacattcc tccgctgatc accgacctct ggtccgcgga cccctcagct       60

catgttttcg aaggcaagct ctgggtttac ccatctcacg acatcgaagc caatgttgtc      120

aacggcacag gaggcgctca atacgccatg agggattacc atacctactc catgaagagc      180

atctatggta aagatcccgt tgtcgaccac ggcgtcgctc tctcagtcga tgacgttccc      240

tgggcgaagc agcaaatgtg ggctcctgac gcagctcata agaacggcaa atattatctg      300

tacttccccg ccaaggacaa ggatgagatc ttcagaattg gagttgctgt ctccaacaag      360

cccagcggtc ctttcaaggc tgacaagagc tggattcctg gcacgtacag tatcgaccct      420

gctagctacg tcgacaatga taaagaggcc tacctcatct ggggcggtat ctggggcggc      480

cagctccaag cctggcagga taaaaagaac tttaacgagt cgtggatcgg agacaaggct      540

gctcctaacg gcaccaatgc cctatcccct cagatcgcca agctaagcaa ggacatgcac      600

aagatcaccg aaacaccccg cgatctcgtc attctcgccc ccgagacagg caagcctctt      660

caggctgagg acaacaagcg acgattcttc gagggccctt ggattcacaa gcgcggcaag      720

ctgtactacc tcatgtactc caccggtgat acccacttcc ttgtctacgc tacttccaag      780

aacatctacg gtccttatac ctaccagggc aagattcttg atcctgttga tgggtggact      840

actcatggaa gtattgttga gtataaggga cagtggtggc ttttctttgc tgatgcgcat      900

acgtctggta aggattacct tcgacaggtg aaggcgagga agatctggta tgacaagaac      960

ggcaagatct tgcttcaccg tccttag                                          987


<210>  7
<211>  105
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 1

<400>  7
gatcctcttc cgtcccatat gtcgttccga tctcttctcg ccctgagcgg ccttgtctgc       60

tcggggttgg cacaaaacac taatgacatt cctccgctga tcacc                      105


<210>  8
<211>  55
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 2

<400>  8
cctgcagccc gggggatccc taaggacggt gaagcaagat cttgccgttc ttgtc            55


