                         SEQUENCE LISTING

<110>  The Regents of the University of California
       Ghajar, Cyrus
       Bissell, Mina J
 
<120>  Scalable Organotypic Models of Tumor Dormancy

<130>  2013-097-02PCT

<140>  PCT/US14/03814
<141>  2014-05-17

<150>  US 61/824,949
<151>  2013-05-17

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic shRNA targeting human Notch1

<400>  1
ccggctttgt ttcaggttca gtattctcga gaatactgaa cctgaaacaa agttttt          57


<210>  2
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic shRNA targeting human Notch1

<400>  2
ccggcgctgc ctggacaaga tcaatctcga gattgatctt gtccaggcag cgttttt          57


<210>  3
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic shRNA targeting human Notch1

<400>  3
ccggcaaaga catgaccagt ggctactcga gtagccactg gtcatgtctt tgttttt          57


<210>  4
<211>  5820
<212>  DNA
<213>  Homo sapiens

<400>  4
agccgctgcg cccgagctgg cctgcgagtt cagggctcct gtcgctctcc aggagcaacc       60

tctactccgg acgcacaggc attccccgcg cccctccagc cctcgccgcc ctcgccaccg      120

ctcccggccg ccgcgctccg gtacacacag gatccctgct gggcaccaac agctccacca      180

tggggctggc ctggggacta ggcgtcctgt tcctgatgca tgtgtgtggc accaaccgca      240

ttccagagtc tggcggagac aacagcgtgt ttgacatctt tgaactcacc ggggccgccc      300

gcaaggggtc tgggcgccga ctggtgaagg gccccgaccc ttccagccca gctttccgca      360

tcgaggatgc caacctgatc ccccctgtgc ctgatgacaa gttccaagac ctggtggatg      420

ctgtgcgggc agaaaagggt ttcctccttc tggcatccct gaggcagatg aagaagaccc      480

ggggcacgct gctggccctg gagcggaaag accactctgg ccaggtcttc agcgtggtgt      540

ccaatggcaa ggcgggcacc ctggacctca gcctgaccgt ccaaggaaag cagcacgtgg      600

tgtctgtgga agaagctctc ctggcaaccg gccagtggaa gagcatcacc ctgtttgtgc      660

aggaagacag ggcccagctg tacatcgact gtgaaaagat ggagaatgct gagttggacg      720

tccccatcca aagcgtcttc accagagacc tggccagcat cgccagactc cgcatcgcaa      780

aggggggcgt caatgacaat ttccaggggg tgctgcagaa tgtgaggttt gtctttggaa      840

ccacaccaga agacatcctc aggaacaaag gctgctccag ctctaccagt gtcctcctca      900

cccttgacaa caacgtggtg aatggttcca gccctgccat ccgcactaac tacattggcc      960

acaagacaaa ggacttgcaa gccatctgcg gcatctcctg tgatgagctg tccagcatgg     1020

tcctggaact caggggcctg cgcaccattg tgaccacgct gcaggacagc atccgcaaag     1080

tgactgaaga gaacaaagag ttggccaatg agctgaggcg gcctccccta tgctatcaca     1140

acggagttca gtacagaaat aacgaggaat ggactgttga tagctgcact gagtgtcact     1200

gtcagaactc agttaccatc tgcaaaaagg tgtcctgccc catcatgccc tgctccaatg     1260

ccacagttcc tgatggagaa tgctgtcctc gctgttggcc cagcgactct gcggacgatg     1320

gctggtctcc atggtccgag tggacctcct gttctacgag ctgtggcaat ggaattcagc     1380

agcgcggccg ctcctgcgat agcctcaaca accgatgtga gggctcctcg gtccagacac     1440

ggacctgcca cattcaggag tgtgacaaga gatttaaaca ggatggtggc tggagccact     1500

ggtccccgtg gtcatcttgt tctgtgacat gtggtgatgg tgtgatcaca aggatccggc     1560

tctgcaactc tcccagcccc cagatgaacg ggaaaccctg tgaaggcgaa gcgcgggaga     1620

ccaaagcctg caagaaagac gcctgcccca tcaatggagg ctggggtcct tggtcaccat     1680

gggacatctg ttctgtcacc tgtggaggag gggtacagaa acgtagtcgt ctctgcaaca     1740

accccacacc ccagtttgga ggcaaggact gcgttggtga tgtaacagaa aaccagatct     1800

gcaacaagca ggactgtcca attgatggat gcctgtccaa tccctgcttt gccggcgtga     1860

agtgtactag ctaccctgat ggcagctgga aatgtggtgc ttgtccccct ggttacagtg     1920

gaaatggcat ccagtgcaca gatgttgatg agtgcaaaga agtgcctgat gcctgcttca     1980

accacaatgg agagcaccgg tgtgagaaca cggaccccgg ctacaactgc ctgccctgcc     2040

ccccacgctt caccggctca cagcccttcg gccagggtgt cgaacatgcc acggccaaca     2100

aacaggtgtg caagccccgt aacccctgca cggatgggac ccacgactgc aacaagaacg     2160

ccaagtgcaa ctacctgggc cactatagcg accccatgta ccgctgcgag tgcaagcctg     2220

gctacgctgg caatggcatc atctgcgggg aggacacaga cctggatggc tggcccaatg     2280

agaacctggt gtgcgtggcc aatgcgactt accactgcaa aaaggataat tgccccaacc     2340

ttcccaactc agggcaggaa gactatgaca aggatggaat tggtgatgcc tgtgatgatg     2400

acgatgacaa tgataaaatt ccagatgaca gggacaactg tccattccat tacaacccag     2460

ctcagtatga ctatgacaga gatgatgtgg gagaccgctg tgacaactgt ccctacaacc     2520

acaacccaga tcaggcagac acagacaaca atggggaagg agacgcctgt gctgcagaca     2580

ttgatggaga cggtatcctc aatgaacggg acaactgcca gtacgtctac aatgtggacc     2640

agagagacac tgatatggat ggggttggag atcagtgtga caattgcccc ttggaacaca     2700

atccggatca gctggactct gactcagacc gcattggaga tacctgtgac aacaatcagg     2760

atattgatga agatggccac cagaacaatc tggacaactg tccctatgtg cccaatgcca     2820

accaggctga ccatgacaaa gatggcaagg gagatgcctg tgaccacgat gatgacaacg     2880

atggcattcc tgatgacaag gacaactgca gactcgtgcc caatcccgac cagaaggact     2940

ctgacggcga tggtcgaggt gatgcctgca aagatgattt tgaccatgac agtgtgccag     3000

acatcgatga catctgtcct gagaatgttg acatcagtga gaccgatttc cgccgattcc     3060

agatgattcc tctggacccc aaagggacat cccaaaatga ccctaactgg gttgtacgcc     3120

atcagggtaa agaactcgtc cagactgtca actgtgatcc tggactcgct gtaggttatg     3180

atgagtttaa tgctgtggac ttcagtggca ccttcttcat caacaccgaa agggacgatg     3240

actatgctgg atttgtcttt ggctaccagt ccagcagccg cttttatgtt gtgatgtgga     3300

agcaagtcac ccagtcctac tgggacacca accccacgag ggctcaggga tactcgggcc     3360

tttctgtgaa agttgtaaac tccaccacag ggcctggcga gcacctgcgg aacgccctgt     3420

ggcacacagg aaacacccct ggccaggtgc gcaccctgtg gcatgaccct cgtcacatag     3480

gctggaaaga tttcaccgcc tacagatggc gtctcagcca caggccaaag acgggtttca     3540

ttagagtggt gatgtatgaa gggaagaaaa tcatggctga ctcaggaccc atctatgata     3600

aaacctatgc tggtggtaga ctagggttgt ttgtcttctc tcaagaaatg gtgttcttct     3660

ctgacctgaa atacgaatgt agagatccct aatcatcaaa ttgttgattg aaagactgat     3720

cataaaccaa tgctggtatt gcaccttctg gaactatggg cttgagaaaa cccccaggat     3780

cacttctcct tggcttcctt cttttctgtg cttgcatcag tgtggactcc tagaacgtgc     3840

gacctgcctc aagaaaatgc agttttcaaa aacagactca gcattcagcc tccaatgaat     3900

aagacatctt ccaagcatat aaacaattgc tttggtttcc ttttgaaaaa gcatctactt     3960

gcttcagttg ggaaggtgcc cattccactc tgcctttgtc acagagcagg gtgctattgt     4020

gaggccatct ctgagcagtg gactcaaaag cattttcagg catgtcagag aagggaggac     4080

tcactagaat tagcaaacaa aaccaccctg acatcctcct tcaggaacac ggggagcaga     4140

ggccaaagca ctaaggggag ggcgcatacc cgagacgatt gtatgaagaa aatatggagg     4200

aactgttaca tgttcggtac taagtcattt tcaggggatt gaaagactat tgctggattt     4260

catgatgctg actggcgtta gctgattaac ccatgtaaat aggcacttaa atagaagcag     4320

gaaagggaga caaagactgg cttctggact tcctccctga tccccaccct tactcatcac     4380

ctgcagtggc cagaattagg gaatcagaat caaaccagtg taaggcagtg ctggctgcca     4440

ttgcctggtc acattgaaat tggtggcttc attctagatg tagcttgtgc agatgtagca     4500

ggaaaatagg aaaacctacc atctcagtga gcaccagctg cctcccaaag gaggggcagc     4560

cgtgcttata tttttatggt tacaatggca caaaattatt atcaacctaa ctaaaacatt     4620

ccttttctct tttttcctga attatcatgg agttttctaa ttctctcttt tggaatgtag     4680

atttttttta aatgctttac gatgtaaaat atttattttt tacttattct ggaagatctg     4740

gctgaaggat tattcatgga acaggaagaa gcgtaaagac tatccatgtc atctttgttg     4800

agagtcttcg tgactgtaag attgtaaata cagattattt attaactctg ttctgcctgg     4860

aaatttaggc ttcatacgga aagtgtttga gagcaagtag ttgacattta tcagcaaatc     4920

tcttgcaaga acagcacaag gaaaatcagt ctaataagct gctctgcccc ttgtgctcag     4980

agtggatgtt atgggattct ttttttctct gttttatctt ttcaagtgga attagttggt     5040

tatccatttg caaatgtttt aaattgcaaa gaaagccatg aggtcttcaa tactgtttta     5100

ccccatccct tgtgcatatt tccagggaga aggaaagcat atacactttt ttctttcatt     5160

tttccaaaag agaaaaaaat gacaaaaggt gaaacttaca tacaaatatt acctcatttg     5220

ttgtgtgact gagtaaagaa tttttggatc aagcggaaag agtttaagtg tctaacaaac     5280

ttaaagctac tgtagtacct aaaaagtcag tgttgtacat agcataaaaa ctctgcagag     5340

aagtattccc aataaggaaa tagcattgaa atgttaaata caatttctga aagttatgtt     5400

ttttttctat catctggtat accattgctt tatttttata aattattttc tcattgccat     5460

tggaatagat atctcagatt gtgtagatat gctatttaaa taatttatca ggaaatactg     5520

cctgtagagt tagtatttct atttttatat aatgtttgca cactgaattg aagaattgtt     5580

ggttttttct tttttttgtt ttgttttttt tttttttttt ttttgctttt gacctcccat     5640

ttttactatt tgccaatacc tttttctagg aatgtgcttt tttttgtaca catttttatc     5700

cattttacat tctaaagcag tgtaagttgt atattactgt ttcttatgta caaggaacaa     5760

caataaatca tatggaaatt tatatttata aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5820


<210>  5
<211>  1170
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Gly Leu Ala Trp Gly Leu Gly Val Leu Phe Leu Met His Val Cys 
1               5                   10                  15      


Gly Thr Asn Arg Ile Pro Glu Ser Gly Gly Asp Asn Ser Val Phe Asp 
            20                  25                  30          


Ile Phe Glu Leu Thr Gly Ala Ala Arg Lys Gly Ser Gly Arg Arg Leu 
        35                  40                  45              


Val Lys Gly Pro Asp Pro Ser Ser Pro Ala Phe Arg Ile Glu Asp Ala 
    50                  55                  60                  


Asn Leu Ile Pro Pro Val Pro Asp Asp Lys Phe Gln Asp Leu Val Asp 
65                  70                  75                  80  


Ala Val Arg Ala Glu Lys Gly Phe Leu Leu Leu Ala Ser Leu Arg Gln 
                85                  90                  95      


Met Lys Lys Thr Arg Gly Thr Leu Leu Ala Leu Glu Arg Lys Asp His 
            100                 105                 110         


Ser Gly Gln Val Phe Ser Val Val Ser Asn Gly Lys Ala Gly Thr Leu 
        115                 120                 125             


Asp Leu Ser Leu Thr Val Gln Gly Lys Gln His Val Val Ser Val Glu 
    130                 135                 140                 


Glu Ala Leu Leu Ala Thr Gly Gln Trp Lys Ser Ile Thr Leu Phe Val 
145                 150                 155                 160 


Gln Glu Asp Arg Ala Gln Leu Tyr Ile Asp Cys Glu Lys Met Glu Asn 
                165                 170                 175     


Ala Glu Leu Asp Val Pro Ile Gln Ser Val Phe Thr Arg Asp Leu Ala 
            180                 185                 190         


Ser Ile Ala Arg Leu Arg Ile Ala Lys Gly Gly Val Asn Asp Asn Phe 
        195                 200                 205             


Gln Gly Val Leu Gln Asn Val Arg Phe Val Phe Gly Thr Thr Pro Glu 
    210                 215                 220                 


Asp Ile Leu Arg Asn Lys Gly Cys Ser Ser Ser Thr Ser Val Leu Leu 
225                 230                 235                 240 


Thr Leu Asp Asn Asn Val Val Asn Gly Ser Ser Pro Ala Ile Arg Thr 
                245                 250                 255     


Asn Tyr Ile Gly His Lys Thr Lys Asp Leu Gln Ala Ile Cys Gly Ile 
            260                 265                 270         


Ser Cys Asp Glu Leu Ser Ser Met Val Leu Glu Leu Arg Gly Leu Arg 
        275                 280                 285             


Thr Ile Val Thr Thr Leu Gln Asp Ser Ile Arg Lys Val Thr Glu Glu 
    290                 295                 300                 


Asn Lys Glu Leu Ala Asn Glu Leu Arg Arg Pro Pro Leu Cys Tyr His 
305                 310                 315                 320 


Asn Gly Val Gln Tyr Arg Asn Asn Glu Glu Trp Thr Val Asp Ser Cys 
                325                 330                 335     


Thr Glu Cys His Cys Gln Asn Ser Val Thr Ile Cys Lys Lys Val Ser 
            340                 345                 350         


Cys Pro Ile Met Pro Cys Ser Asn Ala Thr Val Pro Asp Gly Glu Cys 
        355                 360                 365             


Cys Pro Arg Cys Trp Pro Ser Asp Ser Ala Asp Asp Gly Trp Ser Pro 
    370                 375                 380                 


Trp Ser Glu Trp Thr Ser Cys Ser Thr Ser Cys Gly Asn Gly Ile Gln 
385                 390                 395                 400 


Gln Arg Gly Arg Ser Cys Asp Ser Leu Asn Asn Arg Cys Glu Gly Ser 
                405                 410                 415     


Ser Val Gln Thr Arg Thr Cys His Ile Gln Glu Cys Asp Lys Arg Phe 
            420                 425                 430         


Lys Gln Asp Gly Gly Trp Ser His Trp Ser Pro Trp Ser Ser Cys Ser 
        435                 440                 445             


Val Thr Cys Gly Asp Gly Val Ile Thr Arg Ile Arg Leu Cys Asn Ser 
    450                 455                 460                 


Pro Ser Pro Gln Met Asn Gly Lys Pro Cys Glu Gly Glu Ala Arg Glu 
465                 470                 475                 480 


Thr Lys Ala Cys Lys Lys Asp Ala Cys Pro Ile Asn Gly Gly Trp Gly 
                485                 490                 495     


Pro Trp Ser Pro Trp Asp Ile Cys Ser Val Thr Cys Gly Gly Gly Val 
            500                 505                 510         


Gln Lys Arg Ser Arg Leu Cys Asn Asn Pro Thr Pro Gln Phe Gly Gly 
        515                 520                 525             


Lys Asp Cys Val Gly Asp Val Thr Glu Asn Gln Ile Cys Asn Lys Gln 
    530                 535                 540                 


Asp Cys Pro Ile Asp Gly Cys Leu Ser Asn Pro Cys Phe Ala Gly Val 
545                 550                 555                 560 


Lys Cys Thr Ser Tyr Pro Asp Gly Ser Trp Lys Cys Gly Ala Cys Pro 
                565                 570                 575     


Pro Gly Tyr Ser Gly Asn Gly Ile Gln Cys Thr Asp Val Asp Glu Cys 
            580                 585                 590         


Lys Glu Val Pro Asp Ala Cys Phe Asn His Asn Gly Glu His Arg Cys 
        595                 600                 605             


Glu Asn Thr Asp Pro Gly Tyr Asn Cys Leu Pro Cys Pro Pro Arg Phe 
    610                 615                 620                 


Thr Gly Ser Gln Pro Phe Gly Gln Gly Val Glu His Ala Thr Ala Asn 
625                 630                 635                 640 


Lys Gln Val Cys Lys Pro Arg Asn Pro Cys Thr Asp Gly Thr His Asp 
                645                 650                 655     


Cys Asn Lys Asn Ala Lys Cys Asn Tyr Leu Gly His Tyr Ser Asp Pro 
            660                 665                 670         


Met Tyr Arg Cys Glu Cys Lys Pro Gly Tyr Ala Gly Asn Gly Ile Ile 
        675                 680                 685             


Cys Gly Glu Asp Thr Asp Leu Asp Gly Trp Pro Asn Glu Asn Leu Val 
    690                 695                 700                 


Cys Val Ala Asn Ala Thr Tyr His Cys Lys Lys Asp Asn Cys Pro Asn 
705                 710                 715                 720 


Leu Pro Asn Ser Gly Gln Glu Asp Tyr Asp Lys Asp Gly Ile Gly Asp 
                725                 730                 735     


Ala Cys Asp Asp Asp Asp Asp Asn Asp Lys Ile Pro Asp Asp Arg Asp 
            740                 745                 750         


Asn Cys Pro Phe His Tyr Asn Pro Ala Gln Tyr Asp Tyr Asp Arg Asp 
        755                 760                 765             


Asp Val Gly Asp Arg Cys Asp Asn Cys Pro Tyr Asn His Asn Pro Asp 
    770                 775                 780                 


Gln Ala Asp Thr Asp Asn Asn Gly Glu Gly Asp Ala Cys Ala Ala Asp 
785                 790                 795                 800 


Ile Asp Gly Asp Gly Ile Leu Asn Glu Arg Asp Asn Cys Gln Tyr Val 
                805                 810                 815     


Tyr Asn Val Asp Gln Arg Asp Thr Asp Met Asp Gly Val Gly Asp Gln 
            820                 825                 830         


Cys Asp Asn Cys Pro Leu Glu His Asn Pro Asp Gln Leu Asp Ser Asp 
        835                 840                 845             


Ser Asp Arg Ile Gly Asp Thr Cys Asp Asn Asn Gln Asp Ile Asp Glu 
    850                 855                 860                 


Asp Gly His Gln Asn Asn Leu Asp Asn Cys Pro Tyr Val Pro Asn Ala 
865                 870                 875                 880 


Asn Gln Ala Asp His Asp Lys Asp Gly Lys Gly Asp Ala Cys Asp His 
                885                 890                 895     


Asp Asp Asp Asn Asp Gly Ile Pro Asp Asp Lys Asp Asn Cys Arg Leu 
            900                 905                 910         


Val Pro Asn Pro Asp Gln Lys Asp Ser Asp Gly Asp Gly Arg Gly Asp 
        915                 920                 925             


Ala Cys Lys Asp Asp Phe Asp His Asp Ser Val Pro Asp Ile Asp Asp 
    930                 935                 940                 


Ile Cys Pro Glu Asn Val Asp Ile Ser Glu Thr Asp Phe Arg Arg Phe 
945                 950                 955                 960 


Gln Met Ile Pro Leu Asp Pro Lys Gly Thr Ser Gln Asn Asp Pro Asn 
                965                 970                 975     


Trp Val Val Arg His Gln Gly Lys Glu Leu Val Gln Thr Val Asn Cys 
            980                 985                 990         


Asp Pro Gly Leu Ala Val Gly Tyr  Asp Glu Phe Asn Ala  Val Asp Phe 
        995                 1000                 1005             


Ser Gly  Thr Phe Phe Ile Asn  Thr Glu Arg Asp Asp  Asp Tyr Ala 
    1010                 1015                 1020             


Gly Phe  Val Phe Gly Tyr Gln  Ser Ser Ser Arg Phe  Tyr Val Val 
    1025                 1030                 1035             


Met Trp  Lys Gln Val Thr Gln  Ser Tyr Trp Asp Thr  Asn Pro Thr 
    1040                 1045                 1050             


Arg Ala  Gln Gly Tyr Ser Gly  Leu Ser Val Lys Val  Val Asn Ser 
    1055                 1060                 1065             


Thr Thr  Gly Pro Gly Glu His  Leu Arg Asn Ala Leu  Trp His Thr 
    1070                 1075                 1080             


Gly Asn  Thr Pro Gly Gln Val  Arg Thr Leu Trp His  Asp Pro Arg 
    1085                 1090                 1095             


His Ile  Gly Trp Lys Asp Phe  Thr Ala Tyr Arg Trp  Arg Leu Ser 
    1100                 1105                 1110             


His Arg  Pro Lys Thr Gly Phe  Ile Arg Val Val Met  Tyr Glu Gly 
    1115                 1120                 1125             


Lys Lys  Ile Met Ala Asp Ser  Gly Pro Ile Tyr Asp  Lys Thr Tyr 
    1130                 1135                 1140             


Ala Gly  Gly Arg Leu Gly Leu  Phe Val Phe Ser Gln  Glu Met Val 
    1145                 1150                 1155             


Phe Phe  Ser Asp Leu Lys Tyr  Glu Cys Arg Asp Pro  
    1160                 1165                 1170 


