                         SEQUENCE LISTING

<110>  Syracuse University
 
<120>  METHODS AND SYSTEMS FOR ZINC DELIVERY USING INTRINSIC FACTOR OR 
       HAPTOCORRIN

<130>  156P328A

<150>  61/823594
<151>  2013-05-15

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1308
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human TCN1 gene modified to include a central zinc-binding site

<400>  1
atgcgccaga gccatcagct gcccctggtc ggactgctgc tgtttagttt catcccaagt       60

cagctgtgcg agatttgcga agtgtctgag gaaaattaca tcaggctgaa gccactgctg      120

aacacaatga ttcagagcaa ttataaccgc ggcacttccg ccgtgaatgt ggtcctgtct      180

ctgaagctgg tcggcatcca gattcagact ctgatgcaga aaatgatcca gcagattaag      240

tacaacgtga aaagcagact gtccgatgtc agctccgggg agctggccct gatcattctg      300

gctctgggag tgtgccgcaa tgccgaggaa aacctgatct acgattatca cctgattgac      360

aagctggaga acaagttcca ggcagagatc gaaaatatgg aagcacataa cgggacacca      420

ctgcgagacg cagatgctga cagactgtct ctggacgtgc tggccctgtg cctgttcaat      480

ggcaactact ccaccgctga ggtggtcaac cacttcacac ctgaaaataa gaactactac      540

ttcggctctc agtttagtgt ggatactggg gcaatggccg tgctggctct gacctgtgtc      600

aagaaatccc tgatcaatgg gcagattaag gcagacgaag gaagtctgaa aaacatctca      660

atctacacca agagcctggt ggaaaaaatc ctgagtgaga agaaagaaaa tggactgatt      720

ggcaacactt tctccaccgg cgaggctatg caggcactgt tcgtgtctag tgattactac      780

aacgaaaacg actggaattg ccagcagaca ctgaacactg tgctgaccga gatcagccag      840

ggagcatttt ctaatccaaa cgccgctgca caggtgctgc cagcactgat gggcaagacc      900

ttcctggata ttaacaagga ctcaagctgt gtgtccgctt ctgggaattt taacatcagc      960

gcagatgagc ccattaccgt cacaccccct gacagtcagt catacatctc agtgaattat     1020

agcgtcagga ttaacgagac atacttcact aatgtgaccg tcctgaacgg cagcgtgttt     1080

ctgtctgtca tggaaaaggc tcagaaaatg aacgatacaa tcttcggctt tactatggag     1140

gaacgaagct ggggacctta catcacctgc attcagggcc tgtgtgccaa caataacgac     1200

cggacatatt gggagctgct gagtggcggg gaaccactgt cacagggcgc agggagttat     1260

gtcgtgagaa atggagaaaa tctggaagtg aggtggagca aatactga                  1308


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zinc-binding site protein sequence based on conventional 
       zinc-binding site sequences

<400>  2

Arg Asp Ala Asp Ala Asp Arg 
1               5           


<210>  3
<211>  435
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein expressed by the human TCN1 gene modified to include a 
       central zinc-binding site

<400>  3

Met Arg Gln Ser His Gln Leu Pro Leu Val Gly Leu Leu Leu Phe Ser 
1               5                   10                  15      


Phe Ile Pro Ser Gln Leu Cys Glu Ile Cys Glu Val Ser Glu Glu Asn 
            20                  25                  30          


Tyr Ile Arg Leu Lys Pro Leu Leu Asn Thr Met Ile Gln Ser Asn Tyr 
        35                  40                  45              


Asn Arg Gly Thr Ser Ala Val Asn Val Val Leu Ser Leu Lys Leu Val 
    50                  55                  60                  


Gly Ile Gln Ile Gln Thr Leu Met Gln Lys Met Ile Gln Gln Ile Lys 
65                  70                  75                  80  


Tyr Asn Val Lys Ser Arg Leu Ser Asp Val Ser Ser Gly Glu Leu Ala 
                85                  90                  95      


Leu Ile Ile Leu Ala Leu Gly Val Cys Arg Asn Ala Glu Glu Asn Leu 
            100                 105                 110         


Ile Tyr Asp Tyr His Leu Ile Asp Lys Leu Glu Asn Lys Phe Gln Ala 
        115                 120                 125             


Glu Ile Glu Asn Met Glu Ala His Asn Gly Thr Pro Leu Arg Asp Ala 
    130                 135                 140                 


Asp Ala Asp Arg Leu Ser Leu Asp Val Leu Ala Leu Cys Leu Phe Asn 
145                 150                 155                 160 


Gly Asn Tyr Ser Thr Ala Glu Val Val Asn His Phe Thr Pro Glu Asn 
                165                 170                 175     


Lys Asn Tyr Tyr Phe Gly Ser Gln Phe Ser Val Asp Thr Gly Ala Met 
            180                 185                 190         


Ala Val Leu Ala Leu Thr Cys Val Lys Lys Ser Leu Ile Asn Gly Gln 
        195                 200                 205             


Ile Lys Ala Asp Glu Gly Ser Leu Lys Asn Ile Ser Ile Tyr Thr Lys 
    210                 215                 220                 


Ser Leu Val Glu Lys Ile Leu Ser Glu Lys Lys Glu Asn Gly Leu Ile 
225                 230                 235                 240 


Gly Asn Thr Phe Ser Thr Gly Glu Ala Met Gln Ala Leu Phe Val Ser 
                245                 250                 255     


Ser Asp Tyr Tyr Asn Glu Asn Asp Trp Asn Cys Gln Gln Thr Leu Asn 
            260                 265                 270         


Thr Val Leu Thr Glu Ile Ser Gln Gly Ala Phe Ser Asn Pro Asn Ala 
        275                 280                 285             


Ala Ala Gln Val Leu Pro Ala Leu Met Gly Lys Thr Phe Leu Asp Ile 
    290                 295                 300                 


Asn Lys Asp Ser Ser Cys Val Ser Ala Ser Gly Asn Phe Asn Ile Ser 
305                 310                 315                 320 


Ala Asp Glu Pro Ile Thr Val Thr Pro Pro Asp Ser Gln Ser Tyr Ile 
                325                 330                 335     


Ser Val Asn Tyr Ser Val Arg Ile Asn Glu Thr Tyr Phe Thr Asn Val 
            340                 345                 350         


Thr Val Leu Asn Gly Ser Val Phe Leu Ser Val Met Glu Lys Ala Gln 
        355                 360                 365             


Lys Met Asn Asp Thr Ile Phe Gly Phe Thr Met Glu Glu Arg Ser Trp 
    370                 375                 380                 


Gly Pro Tyr Ile Thr Cys Ile Gln Gly Leu Cys Ala Asn Asn Asn Asp 
385                 390                 395                 400 


Arg Thr Tyr Trp Glu Leu Leu Ser Gly Gly Glu Pro Leu Ser Gln Gly 
                405                 410                 415     


Ala Gly Ser Tyr Val Val Arg Asn Gly Glu Asn Leu Glu Val Arg Trp 
            420                 425                 430         


Ser Lys Tyr 
        435 


<210>  4
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human TCN1 gene modified to include SmtA metallothionein 
       zinc-binding sequence

<400>  4
atgcgtcaaa gccatcaact gccgctggtc ggtctgctgc tgttctcgtt tatcccgtca       60

caactgtgcg aaatctgcga agtctctgaa gaaaattata ttcgtctgaa accgctgctg      120

aacaccatga tccagtctaa ttacaaccgc ggcacgagtg ccgttaatgt ggttctgtcc      180

ctgaaactgg tcggtattca gatccaaacc ctgatgcaaa aaatgatcca gcaaatcaaa      240

tacaacgtca aatcacgtct gtcggatgtg agctctggcg aactggccct gattatcctg      300

gcactgggtg tgtgccgcaa tgcagaagaa aacctgattt atgattacca tctgatcgac      360

aaactggaaa acaaattcca ggctgaaatc gaaaacatgg aagcgcacaa cggtaccccg      420

ctgcgtatga cgtcaaccac gctggtgaaa tgcgcctgtg aaccgtgcct gtgtaatgtt      480

gatccgagca aagcaattga ccgcaacggc ctgtattact gctcagaagc ttgtgcggat      540

ggtcataccg gcggttcgaa aggctgcggt catacgggct gcaattgtca cggtcgtctg      600

agtctggacg tgctggctct gtgtctgttt aatggcaact attccaccgc ggaagtcgtg      660

aaccacttca cgccggaaaa caaaaactat tactttggca gccagttctc tgttgatacc      720

ggtgccatgg cagtgctggc cctgacgtgt gtgaaaaaat ctctgattaa tggccaaatc      780

aaagcagacg aaggtagcct gaaaaacatt tctatctaca ccaaaagtct ggtggaaaaa      840

atcctgagcg agaaaaaaga aaacggcctg atcggtaaca ccttttctac gggtgaagct      900

atgcaggcgc tgttcgttag ttccgattat tacaatgaaa acgactggaa ttgccagcaa      960

accctgaaca cggttctgac cgaaatttcc cagggcgctt tttcaaatcc gaacgcagca     1020

gcacaagtcc tgccggcact gatgggtaaa acctttctgg atatcaacaa agactcatcg     1080

tgtgtcagcg cctctggcaa tttcaacatt agcgcagatg aaccgatcac cgtgacgccg     1140

ccggacagtc agtcctatat tagtgtcaat tactccgtgc gcatcaacga aacgtatttt     1200

accaatgtga cggttctgaa cggttcagtt ttcctgtcgg tcatggaaaa agcccagaaa     1260

atgaacgata ccatttttgg cttcacgatg gaagaacgta gctggggccc gtatattacc     1320

tgcatccagg gtctgtgtgc taacaataac gatcgcacct actgggaact gctgtctggt     1380

ggtgaaccgc tgtcgcaagg tgcaggtagc tatgttgtcc gcaatggcga aaacctggaa     1440

gtccgttgga gcaaatacca ccaccaccac caccactaa                            1479


<210>  5
<211>  56
<212>  PRT
<213>  Unknown

<220>
<223>  Synechococcus species

<400>  5

Met Thr Ser Thr Thr Leu Val Lys Cys Ala Cys Glu Pro Cys Leu Cys 
1               5                   10                  15      


Asn Val Asp Pro Ser Lys Ala Ile Asp Arg Asn Gly Leu Tyr Tyr Cys 
            20                  25                  30          


Ser Glu Ala Cys Ala Asp Gly His Thr Gly Gly Ser Lys Gly Cys Gly 
        35                  40                  45              


His Thr Gly Cys Asn Cys His Gly 
    50                  55      


<210>  6
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein expressed by the human TCN1 gene modified to include SmtA
       metallothionein zinc-binding sequence

<400>  6

Met Arg Gln Ser His Gln Leu Pro Leu Val Gly Leu Leu Leu Phe Ser 
1               5                   10                  15      


Phe Ile Pro Ser Gln Leu Cys Glu Ile Cys Glu Val Ser Glu Glu Asn 
            20                  25                  30          


Tyr Ile Arg Leu Lys Pro Leu Leu Asn Thr Met Ile Gln Ser Asn Tyr 
        35                  40                  45              


Asn Arg Gly Thr Ser Ala Val Asn Val Val Leu Ser Leu Lys Leu Val 
    50                  55                  60                  


Gly Ile Gln Ile Gln Thr Leu Met Gln Lys Met Ile Gln Gln Ile Lys 
65                  70                  75                  80  


Tyr Asn Val Lys Ser Arg Leu Ser Asp Val Ser Ser Gly Glu Leu Ala 
                85                  90                  95      


Leu Ile Ile Leu Ala Leu Gly Val Cys Arg Asn Ala Glu Glu Asn Leu 
            100                 105                 110         


Ile Tyr Asp Tyr His Leu Ile Asp Lys Leu Glu Asn Lys Phe Gln Ala 
        115                 120                 125             


Glu Ile Glu Asn Met Glu Ala His Asn Gly Thr Pro Leu Arg Met Thr 
    130                 135                 140                 


Ser Thr Thr Leu Val Lys Cys Ala Cys Glu Pro Cys Leu Cys Asn Val 
145                 150                 155                 160 


Asp Pro Ser Lys Ala Ile Asp Arg Asn Gly Leu Tyr Tyr Cys Ser Glu 
                165                 170                 175     


Ala Cys Ala Asp Gly His Thr Gly Gly Ser Lys Gly Cys Gly His Thr 
            180                 185                 190         


Gly Cys Asn Cys His Gly Arg Leu Ser Leu Asp Val Leu Ala Leu Cys 
        195                 200                 205             


Leu Phe Asn Gly Asn Tyr Ser Thr Ala Glu Val Val Asn His Phe Thr 
    210                 215                 220                 


Pro Glu Asn Lys Asn Tyr Tyr Phe Gly Ser Gln Phe Ser Val Asp Thr 
225                 230                 235                 240 


Gly Ala Met Ala Val Leu Ala Leu Thr Cys Val Lys Lys Ser Leu Ile 
                245                 250                 255     


Asn Gly Gln Ile Lys Ala Asp Glu Gly Ser Leu Lys Asn Ile Ser Ile 
            260                 265                 270         


Tyr Thr Lys Ser Leu Val Glu Lys Ile Leu Ser Glu Lys Lys Glu Asn 
        275                 280                 285             


Gly Leu Ile Gly Asn Thr Phe Ser Thr Gly Glu Ala Met Gln Ala Leu 
    290                 295                 300                 


Phe Val Ser Ser Asp Tyr Tyr Asn Glu Asn Asp Trp Asn Cys Gln Gln 
305                 310                 315                 320 


Thr Leu Asn Thr Val Leu Thr Glu Ile Ser Gln Gly Ala Phe Ser Asn 
                325                 330                 335     


Pro Asn Ala Ala Ala Gln Val Leu Pro Ala Leu Met Gly Lys Thr Phe 
            340                 345                 350         


Leu Asp Ile Asn Lys Asp Ser Ser Cys Val Ser Ala Ser Gly Asn Phe 
        355                 360                 365             


Asn Ile Ser Ala Asp Glu Pro Ile Thr Val Thr Pro Pro Asp Ser Gln 
    370                 375                 380                 


Ser Tyr Ile Ser Val Asn Tyr Ser Val Arg Ile Asn Glu Thr Tyr Phe 
385                 390                 395                 400 


Thr Asn Val Thr Val Leu Asn Gly Ser Val Phe Leu Ser Val Met Glu 
                405                 410                 415     


Lys Ala Gln Lys Met Asn Asp Thr Ile Phe Gly Phe Thr Met Glu Glu 
            420                 425                 430         


Arg Ser Trp Gly Pro Tyr Ile Thr Cys Ile Gln Gly Leu Cys Ala Asn 
        435                 440                 445             


Asn Asn Asp Arg Thr Tyr Trp Glu Leu Leu Ser Gly Gly Glu Pro Leu 
    450                 455                 460                 


Ser Gln Gly Ala Gly Ser Tyr Val Val Arg Asn Gly Glu Asn Leu Glu 
465                 470                 475                 480 


Val Arg Trp Ser Lys Tyr His His His His His His 
                485                 490         


