                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Stabilized lipase in water-soluble films

<130>  12936-WO-PCT

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  807
<212>  DNA
<213>  Thermomyces lanuginosus


<220>
<221>  CDS
<222>  (1)..(807)

<220>
<221>  mat_peptide
<222>  (1)..()

<400>  1
gag gtc tcg cag gat ctg ttt aac cag ttc aat ctc ttt gca cag tat         48
Glu Val Ser Gln Asp Leu Phe Asn Gln Phe Asn Leu Phe Ala Gln Tyr           
1               5                   10                  15                

tct gca gcc gca tac tgc gga aaa aac aat gat gcc cca gct ggt aca         96
Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn Asp Ala Pro Ala Gly Thr           
            20                  25                  30                    

aac att acg tgc acg gga aat gcc tgc ccc gag gta gag aag gcg gat        144
Asn Ile Thr Cys Thr Gly Asn Ala Cys Pro Glu Val Glu Lys Ala Asp           
        35                  40                  45                        

gca acg ttt ctc tac tcg ttt gaa gac tct gga gtg ggc gat gtc acc        192
Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser Gly Val Gly Asp Val Thr           
    50                  55                  60                            

ggc ttc ctt gct ctc gac aac acg aac aaa ttg atc gtc ctc tct ttc        240
Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys Leu Ile Val Leu Ser Phe           
65                  70                  75                  80            

cgt ggc tct cgt tcc ata gag aac tgg atc ggg aat ctt aac ttc gac        288
Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile Gly Asn Leu Asn Phe Asp           
                85                  90                  95                

ttg aaa gaa ata aat gac att tgc tcc ggc tgc agg gga cat gac ggc        336
Leu Lys Glu Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Asp Gly           
            100                 105                 110                   

ttc act tcg tcc tgg agg tct gta gcc gat acg tta agg cag aag gtg        384
Phe Thr Ser Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val           
        115                 120                 125                       

gag gat gct gtg agg gag cat ccc gac tat cgc gtg gtg ttt acc gga        432
Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly           
    130                 135                 140                           

cat agc ttg ggt ggt gca ttg gca act gtt gcc gga gca gac ctg cgt        480
His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp Leu Arg           
145                 150                 155                 160           

gga aat ggg tat gat atc gac gtg ttt tca tat ggc gcc ccc cga gtc        528
Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser Tyr Gly Ala Pro Arg Val           
                165                 170                 175               

gga aac agg gct ttt gca gaa ttc ctg acc gta cag acc ggc gga aca        576
Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr Val Gln Thr Gly Gly Thr           
            180                 185                 190                   

ctc tac cgc att acc cac acc aat gat att gtc cct aga ctc ccg ccg        624
Leu Tyr Arg Ile Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro           
        195                 200                 205                       

cgc gaa ttc ggt tac agc cat tct agc cca gag tac tgg atc aaa tct        672
Arg Glu Phe Gly Tyr Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser           
    210                 215                 220                           

gga acc ctt gtc ccc gtc acc cga aac gat atc gtg aag ata gaa ggc        720
Gly Thr Leu Val Pro Val Thr Arg Asn Asp Ile Val Lys Ile Glu Gly           
225                 230                 235                 240           

atc gat gcc acc ggc ggc aat aac cag cct aac att ccg gat atc cct        768
Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Asp Ile Pro           
                245                 250                 255               

gcg cac cta tgg tac ttc ggg tta att ggg aca tgt ctt                    807
Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr Cys Leu                       
            260                 265                                       


<210>  2
<211>  269
<212>  PRT
<213>  Thermomyces lanuginosus

<400>  2

Glu Val Ser Gln Asp Leu Phe Asn Gln Phe Asn Leu Phe Ala Gln Tyr 
1               5                   10                  15      


Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn Asp Ala Pro Ala Gly Thr 
            20                  25                  30          


Asn Ile Thr Cys Thr Gly Asn Ala Cys Pro Glu Val Glu Lys Ala Asp 
        35                  40                  45              


Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser Gly Val Gly Asp Val Thr 
    50                  55                  60                  


Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys Leu Ile Val Leu Ser Phe 
65                  70                  75                  80  


Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile Gly Asn Leu Asn Phe Asp 
                85                  90                  95      


Leu Lys Glu Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Asp Gly 
            100                 105                 110         


Phe Thr Ser Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val 
        115                 120                 125             


Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly 
    130                 135                 140                 


His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp Leu Arg 
145                 150                 155                 160 


Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser Tyr Gly Ala Pro Arg Val 
                165                 170                 175     


Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr Val Gln Thr Gly Gly Thr 
            180                 185                 190         


Leu Tyr Arg Ile Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro 
        195                 200                 205             


Arg Glu Phe Gly Tyr Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser 
    210                 215                 220                 


Gly Thr Leu Val Pro Val Thr Arg Asn Asp Ile Val Lys Ile Glu Gly 
225                 230                 235                 240 


Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Asp Ile Pro 
                245                 250                 255     


Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr Cys Leu 
            260                 265                 


<210>  3
<211>  269
<212>  PRT
<213>  Artificial

<220>
<223>  Thermomyces lanuginosus lipase variant

<400>  3

Glu Val Ser Gln Asp Leu Phe Asn Gln Phe Asn Leu Phe Ala Gln Tyr 
1               5                   10                  15      


Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn Asp Ala Pro Ala Gly Thr 
            20                  25                  30          


Asn Ile Thr Cys Thr Gly Asn Ala Cys Pro Glu Val Glu Lys Ala Asp 
        35                  40                  45              


Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser Gly Val Gly Asp Val Thr 
    50                  55                  60                  


Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys Leu Ile Val Leu Ser Phe 
65                  70                  75                  80  


Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile Gly Asn Leu Asn Phe Asp 
                85                  90                  95      


Leu Lys Glu Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Asp Gly 
            100                 105                 110         


Phe Thr Ser Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val 
        115                 120                 125             


Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly 
    130                 135                 140                 


His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp Leu Arg 
145                 150                 155                 160 


Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser Tyr Gly Ala Pro Arg Val 
                165                 170                 175     


Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr Val Gln Thr Gly Gly Thr 
            180                 185                 190         


Leu Tyr Arg Ile Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro 
        195                 200                 205             


Arg Glu Phe Gly Tyr Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser 
    210                 215                 220                 


Gly Thr Leu Val Pro Val Arg Arg Arg Asp Ile Val Lys Ile Glu Gly 
225                 230                 235                 240 


Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Asp Ile Pro 
                245                 250                 255     


Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr Cys Leu 
            260                 265                 


<210>  4
<211>  269
<212>  PRT
<213>  Artificial

<220>
<223>  Thermomyces lanuginosus lipase variant

<400>  4

Glu Val Ser Gln Asp Leu Phe Asn Gln Phe Asn Leu Phe Ala Gln Tyr 
1               5                   10                  15      


Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn Arg Ala Pro Ala Gly Thr 
            20                  25                  30          


Asn Ile Thr Cys Thr Ala Asn Ala Cys Pro Glu Val Glu Lys Ala Asp 
        35                  40                  45              


Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser Gly Val Gly Asp Val Thr 
    50                  55                  60                  


Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys Leu Ile Val Leu Ser Phe 
65                  70                  75                  80  


Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile Gly Asn Leu Asn Phe Glu 
                85                  90                  95      


Leu Lys Glu Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Ala Gly 
            100                 105                 110         


Phe Thr Ser Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val 
        115                 120                 125             


Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly 
    130                 135                 140                 


His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp Leu Arg 
145                 150                 155                 160 


Gly Asn Lys Tyr Asp Ile Asp Val Phe Ser Tyr Gly Ala Pro Arg Val 
                165                 170                 175     


Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr Val Gln Thr Gly Gly Thr 
            180                 185                 190         


Leu Tyr Arg Ile Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro 
        195                 200                 205             


Arg Glu Phe Gly Tyr Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser 
    210                 215                 220                 


Gly Thr Leu Val Pro Val Arg Arg Arg Asp Ile Val Lys Ile Glu Gly 
225                 230                 235                 240 


Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Ser Ile Thr 
                245                 250                 255     


Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr Cys Leu 
            260                 265                 


