                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Process for simultaneous saccharification and fermentation of 
       whey permeate

<130>  12615-WO-PCT

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  3996
<212>  DNA
<213>  Bifidobacterium bifidum

<400>  1
atgaagaaac cgttggggaa aattgtcgca agcaccgcac tactcatttc tgttgctttt       60

agttcatcga tcgcatcggc tgttgaggac gccacgagaa gtgatagcac cactcaaatg      120

tcatccacac cggaagtagt gtattcttca gcagtcgatt ccaaacagaa tagaacatct      180

gactttgatg ccaattggaa attcatgctt tcggatagcg tacaggcgca ggatccggca      240

tttgatgatt ctgcgtggca gcaggttgat ctgcctcatg actatagcat tacacagaaa      300

tacagccagt ctaatgaagc ggaatcggcg tacttacctg ggggaacagg atggtataga      360

aaatctttca caattgacag ggacttagcg ggtaagcgga tcgcgatcaa ctttgatgga      420

gtctatatga atgcgactgt gtggttcaat ggcgtgaagt tggggacaca cccatatggg      480

tattccccct tttcatttga cctcactggg aacgcgaagt ttgggggaga gaataccatt      540

gttgtgaaag tcgaaaacag attgccgtca tcccgctggt actctggttc aggcatttac      600

cgcgatgtaa ctttaaccgt gacggacgga gtccatgttg ggaacaatgg tgttgctatc      660

aaaacaccaa gcttggcaac tcagaatgga ggcaatgtga ctatgaacct cacaacaaaa      720

gtggccaacg acacaaaagc agcagccaat atcacactta aacaaaccgt gtttccgaag      780

ggaggcaaga cggacgctgc aataggaaca gtcacgacgg catctaagtc cattgcagca      840

ggagcgtccg cagatgtgac atcaacaatt acggcagcgt cacctaaact gtggtcgata      900

aagaatccga atttgtacac agtgcgcacc gaggtgttaa acggaggtaa agttttggac      960

acgtatgata cagaatatgg gtttcgctgg actggatttg atgcaacatc aggattcagc     1020

ttaaacggag agaaggttaa acttaaagga gtgtcaatgc atcacgatca gggttctttg     1080

ggagcggttg cgaatcggag agccatcgaa cgacaggtcg aaatcttaca gaaaatggga     1140

gtcaattcaa tccggacgac acacaatccg gcagcaaaag ccttgattga cgtatgtaac     1200

gaaaaaggag tattagtggt tgaagaagtc tttgacatgt ggaaccgctc caagaatgga     1260

aatacagaag actatggaaa gtggtttggt caagccatag caggggataa cgctgtcttg     1320

ggtggagata aggacgaaac gtgggcgaag tttgacttaa catcaacaat caatcgcgat     1380

cgcaacgctc cgagtgtgat aatgtggtcg cttggcaacg agatgatgga aggtattagc     1440

ggatctgtat cggggtttcc ggctacttcc gcaaaactcg tggcatggac aaaagcggct     1500

gattccactc gacctatgac ttatggagac aacaagatca aagctaactg gaatgagtcg     1560

aatacgatgg gagacaactt aacggcaaat gggggagtcg tcggaacgaa ctattccgat     1620

ggcgctaact atgacaaaat ccggacaact catccctctt gggcgatcta tggctctgaa     1680

acagccagtg ctatcaacag caggggaatc tataacagaa caactggcgg agcccaaagc     1740

tccgacaaac agcttacgtc atacgacaac agcgcagttg gctggggtgc agtagcctca     1800

agcgcctggt atgatgtggt ccagcgcgac tttgttgcag gaacttatgt gtggactggt     1860

tttgactact taggcgaacc gacgccttgg aacggtactg gctcaggcgc tgtcggctca     1920

tggccgtccc cgaaaaactc ttactttggg attgtggata cagcaggctt tccgaaagac     1980

acgtactact tttaccaatc tcaatggaac gatgatgtcc atacgcttca tatcttacct     2040

gcgtggaacg aaaatgtcgt ggcgaaaggc tccggcaaca atgtgccggt tgtagtgtat     2100

acagatgcgg ctaaagtaaa actgtacttt actcctaagg gctcgacgga aaaacgattg     2160

atcggggaga aaagtttcac gaagaaaacc acagccgctg gctacacgta ccaggtgtac     2220

gagggagcgg acaaagatag cacggctcac aaaaacatgt atctgacttg gaatgttccg     2280

tgggcagaag ggacgatatc agccgaagca tacgatgaga acaaccgctt aatccccgaa     2340

ggcagcacgg aagggaatgc ctctgttaca acgacaggca aagcagcaaa gttgaaggcg     2400

gatgcagatc ggaaaacgat taccgcagat ggcaaggatc tttcctatat cgaggtggac     2460

gtcacagatg ccaatggaca tatcgtgccg gatgcagcga atcgtgttac atttgacgta     2520

aaaggggcag gcaaactggt tggggttgac aacgggtcgt caccggacca tgatagttat     2580

caggctgaca ataggaaagc gtttagcggc aaagttttag ccatcgtgca gtcaactaaa     2640

gaagccggag aaatcacagt cacagcaaaa gctgacggat tacaatcttc gacggtcaag     2700

attgctacaa ctgcggttcc gggtacatca acggaaaaga cggttaggtc cttctactat     2760

agtagaaact actatgtgaa aactggcaac aaacctatcc tgccaagtga tgtggaggtt     2820

agatattcag acggcacatc cgatcgtcaa aacgtcacgt gggatgctgt ttcagatgat     2880

caaatcgcga aggctggctc tttttcagta gcgggaacag tggctggaca gaaaatctca     2940

gtccgtgtaa ctatgattga cgagattggg gcactgttga actattcagc ttcaacaccg     3000

gtaggaacac cagctgtttt gccaggctca aggccagcgg tactcccaga cggcacggtt     3060

acatctgcaa actttgctgt agattggacg aaacctgccg acaccgtgta caatacagct     3120

gggactgtga aagtgcctgg gacagcgaca gtctttggaa aagagttcaa agtcacagcg     3180

acaatcagag tacagagatc gcaggtcacg atcggaagtt ccgtctcagg taatgcactg     3240

agacttacgc aaaacattcc ggctgacaaa cagagtgata cacttgatgc aatcaaagac     3300

ggaagcacaa cagttgatgc aaacacgggt ggaggggcta atccgtccgc gtggacgaac     3360

tgggcttaca gcaaagcagg acacaatact gcagaaatca cgtttgagta tgcaacagag     3420

caacagctgg gccaaattgt aatgtacttc tttagagatt caaatgcggt acggtttccc     3480

gatgctggta aaaccaaaat ccagatatcg gcagatggca agaattggac agaccttgcg     3540

gcaacggaaa cgattgccgc tcaggagagt agtgaacgcg ttaaacctta tacatacgac     3600

tttgcaccgg ttggcgcaac ctttgtgaaa gttacggtga cgaacgccga tacaacaacg     3660

ccgtcaggcg tagtttgtgc aggattaaca gaaatagagt taaagacggc aacaagcaag     3720

ttcgtcacaa acacttctgc ggcattatct tcgctgacgg ttaacgggac caaagtctca     3780

gacagcgtgc ttgcagctgg atcatacaac actcctgcca ttatcgcgga tgtaaaagcg     3840

gaaggcgaag gtaatgcgtc tgtcactgtg ttgcctgctc acgataacgt catccgtgtg     3900

attacagagt ccgaagacca tgttacaaga aaaacgttta ccattaactt ggggacagaa     3960

caagaatttc ctgccgattc agatgaaaga gattaa                               3996


<210>  2
<211>  1331
<212>  PRT
<213>  Bifidobacterium bifidum

<400>  2

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
1               5                   10                  15      


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Val Glu Asp Ala Thr 
            20                  25                  30          


Arg Ser Asp Ser Thr Thr Gln Met Ser Ser Thr Pro Glu Val Val Tyr 
        35                  40                  45              


Ser Ser Ala Val Asp Ser Lys Gln Asn Arg Thr Ser Asp Phe Asp Ala 
    50                  55                  60                  


Asn Trp Lys Phe Met Leu Ser Asp Ser Val Gln Ala Gln Asp Pro Ala 
65                  70                  75                  80  


Phe Asp Asp Ser Ala Trp Gln Gln Val Asp Leu Pro His Asp Tyr Ser 
                85                  90                  95      


Ile Thr Gln Lys Tyr Ser Gln Ser Asn Glu Ala Glu Ser Ala Tyr Leu 
            100                 105                 110         


Pro Gly Gly Thr Gly Trp Tyr Arg Lys Ser Phe Thr Ile Asp Arg Asp 
        115                 120                 125             


Leu Ala Gly Lys Arg Ile Ala Ile Asn Phe Asp Gly Val Tyr Met Asn 
    130                 135                 140                 


Ala Thr Val Trp Phe Asn Gly Val Lys Leu Gly Thr His Pro Tyr Gly 
145                 150                 155                 160 


Tyr Ser Pro Phe Ser Phe Asp Leu Thr Gly Asn Ala Lys Phe Gly Gly 
                165                 170                 175     


Glu Asn Thr Ile Val Val Lys Val Glu Asn Arg Leu Pro Ser Ser Arg 
            180                 185                 190         


Trp Tyr Ser Gly Ser Gly Ile Tyr Arg Asp Val Thr Leu Thr Val Thr 
        195                 200                 205             


Asp Gly Val His Val Gly Asn Asn Gly Val Ala Ile Lys Thr Pro Ser 
    210                 215                 220                 


Leu Ala Thr Gln Asn Gly Gly Asn Val Thr Met Asn Leu Thr Thr Lys 
225                 230                 235                 240 


Val Ala Asn Asp Thr Lys Ala Ala Ala Asn Ile Thr Leu Lys Gln Thr 
                245                 250                 255     


Val Phe Pro Lys Gly Gly Lys Thr Asp Ala Ala Ile Gly Thr Val Thr 
            260                 265                 270         


Thr Ala Ser Lys Ser Ile Ala Ala Gly Ala Ser Ala Asp Val Thr Ser 
        275                 280                 285             


Thr Ile Thr Ala Ala Ser Pro Lys Leu Trp Ser Ile Lys Asn Pro Asn 
    290                 295                 300                 


Leu Tyr Thr Val Arg Thr Glu Val Leu Asn Gly Gly Lys Val Leu Asp 
305                 310                 315                 320 


Thr Tyr Asp Thr Glu Tyr Gly Phe Arg Trp Thr Gly Phe Asp Ala Thr 
                325                 330                 335     


Ser Gly Phe Ser Leu Asn Gly Glu Lys Val Lys Leu Lys Gly Val Ser 
            340                 345                 350         


Met His His Asp Gln Gly Ser Leu Gly Ala Val Ala Asn Arg Arg Ala 
        355                 360                 365             


Ile Glu Arg Gln Val Glu Ile Leu Gln Lys Met Gly Val Asn Ser Ile 
    370                 375                 380                 


Arg Thr Thr His Asn Pro Ala Ala Lys Ala Leu Ile Asp Val Cys Asn 
385                 390                 395                 400 


Glu Lys Gly Val Leu Val Val Glu Glu Val Phe Asp Met Trp Asn Arg 
                405                 410                 415     


Ser Lys Asn Gly Asn Thr Glu Asp Tyr Gly Lys Trp Phe Gly Gln Ala 
            420                 425                 430         


Ile Ala Gly Asp Asn Ala Val Leu Gly Gly Asp Lys Asp Glu Thr Trp 
        435                 440                 445             


Ala Lys Phe Asp Leu Thr Ser Thr Ile Asn Arg Asp Arg Asn Ala Pro 
    450                 455                 460                 


Ser Val Ile Met Trp Ser Leu Gly Asn Glu Met Met Glu Gly Ile Ser 
465                 470                 475                 480 


Gly Ser Val Ser Gly Phe Pro Ala Thr Ser Ala Lys Leu Val Ala Trp 
                485                 490                 495     


Thr Lys Ala Ala Asp Ser Thr Arg Pro Met Thr Tyr Gly Asp Asn Lys 
            500                 505                 510         


Ile Lys Ala Asn Trp Asn Glu Ser Asn Thr Met Gly Asp Asn Leu Thr 
        515                 520                 525             


Ala Asn Gly Gly Val Val Gly Thr Asn Tyr Ser Asp Gly Ala Asn Tyr 
    530                 535                 540                 


Asp Lys Ile Arg Thr Thr His Pro Ser Trp Ala Ile Tyr Gly Ser Glu 
545                 550                 555                 560 


Thr Ala Ser Ala Ile Asn Ser Arg Gly Ile Tyr Asn Arg Thr Thr Gly 
                565                 570                 575     


Gly Ala Gln Ser Ser Asp Lys Gln Leu Thr Ser Tyr Asp Asn Ser Ala 
            580                 585                 590         


Val Gly Trp Gly Ala Val Ala Ser Ser Ala Trp Tyr Asp Val Val Gln 
        595                 600                 605             


Arg Asp Phe Val Ala Gly Thr Tyr Val Trp Thr Gly Phe Asp Tyr Leu 
    610                 615                 620                 


Gly Glu Pro Thr Pro Trp Asn Gly Thr Gly Ser Gly Ala Val Gly Ser 
625                 630                 635                 640 


Trp Pro Ser Pro Lys Asn Ser Tyr Phe Gly Ile Val Asp Thr Ala Gly 
                645                 650                 655     


Phe Pro Lys Asp Thr Tyr Tyr Phe Tyr Gln Ser Gln Trp Asn Asp Asp 
            660                 665                 670         


Val His Thr Leu His Ile Leu Pro Ala Trp Asn Glu Asn Val Val Ala 
        675                 680                 685             


Lys Gly Ser Gly Asn Asn Val Pro Val Val Val Tyr Thr Asp Ala Ala 
    690                 695                 700                 


Lys Val Lys Leu Tyr Phe Thr Pro Lys Gly Ser Thr Glu Lys Arg Leu 
705                 710                 715                 720 


Ile Gly Glu Lys Ser Phe Thr Lys Lys Thr Thr Ala Ala Gly Tyr Thr 
                725                 730                 735     


Tyr Gln Val Tyr Glu Gly Ala Asp Lys Asp Ser Thr Ala His Lys Asn 
            740                 745                 750         


Met Tyr Leu Thr Trp Asn Val Pro Trp Ala Glu Gly Thr Ile Ser Ala 
        755                 760                 765             


Glu Ala Tyr Asp Glu Asn Asn Arg Leu Ile Pro Glu Gly Ser Thr Glu 
    770                 775                 780                 


Gly Asn Ala Ser Val Thr Thr Thr Gly Lys Ala Ala Lys Leu Lys Ala 
785                 790                 795                 800 


Asp Ala Asp Arg Lys Thr Ile Thr Ala Asp Gly Lys Asp Leu Ser Tyr 
                805                 810                 815     


Ile Glu Val Asp Val Thr Asp Ala Asn Gly His Ile Val Pro Asp Ala 
            820                 825                 830         


Ala Asn Arg Val Thr Phe Asp Val Lys Gly Ala Gly Lys Leu Val Gly 
        835                 840                 845             


Val Asp Asn Gly Ser Ser Pro Asp His Asp Ser Tyr Gln Ala Asp Asn 
    850                 855                 860                 


Arg Lys Ala Phe Ser Gly Lys Val Leu Ala Ile Val Gln Ser Thr Lys 
865                 870                 875                 880 


Glu Ala Gly Glu Ile Thr Val Thr Ala Lys Ala Asp Gly Leu Gln Ser 
                885                 890                 895     


Ser Thr Val Lys Ile Ala Thr Thr Ala Val Pro Gly Thr Ser Thr Glu 
            900                 905                 910         


Lys Thr Val Arg Ser Phe Tyr Tyr Ser Arg Asn Tyr Tyr Val Lys Thr 
        915                 920                 925             


Gly Asn Lys Pro Ile Leu Pro Ser Asp Val Glu Val Arg Tyr Ser Asp 
    930                 935                 940                 


Gly Thr Ser Asp Arg Gln Asn Val Thr Trp Asp Ala Val Ser Asp Asp 
945                 950                 955                 960 


Gln Ile Ala Lys Ala Gly Ser Phe Ser Val Ala Gly Thr Val Ala Gly 
                965                 970                 975     


Gln Lys Ile Ser Val Arg Val Thr Met Ile Asp Glu Ile Gly Ala Leu 
            980                 985                 990         


Leu Asn Tyr Ser Ala Ser Thr Pro  Val Gly Thr Pro Ala  Val Leu Pro 
        995                 1000                 1005             


Gly Ser  Arg Pro Ala Val Leu  Pro Asp Gly Thr Val  Thr Ser Ala 
    1010                 1015                 1020             


Asn Phe  Ala Val Asp Trp Thr  Lys Pro Ala Asp Thr  Val Tyr Asn 
    1025                 1030                 1035             


Thr Ala  Gly Thr Val Lys Val  Pro Gly Thr Ala Thr  Val Phe Gly 
    1040                 1045                 1050             


Lys Glu  Phe Lys Val Thr Ala  Thr Ile Arg Val Gln  Arg Ser Gln 
    1055                 1060                 1065             


Val Thr  Ile Gly Ser Ser Val  Ser Gly Asn Ala Leu  Arg Leu Thr 
    1070                 1075                 1080             


Gln Asn  Ile Pro Ala Asp Lys  Gln Ser Asp Thr Leu  Asp Ala Ile 
    1085                 1090                 1095             


Lys Asp  Gly Ser Thr Thr Val  Asp Ala Asn Thr Gly  Gly Gly Ala 
    1100                 1105                 1110             


Asn Pro  Ser Ala Trp Thr Asn  Trp Ala Tyr Ser Lys  Ala Gly His 
    1115                 1120                 1125             


Asn Thr  Ala Glu Ile Thr Phe  Glu Tyr Ala Thr Glu  Gln Gln Leu 
    1130                 1135                 1140             


Gly Gln  Ile Val Met Tyr Phe  Phe Arg Asp Ser Asn  Ala Val Arg 
    1145                 1150                 1155             


Phe Pro  Asp Ala Gly Lys Thr  Lys Ile Gln Ile Ser  Ala Asp Gly 
    1160                 1165                 1170             


Lys Asn  Trp Thr Asp Leu Ala  Ala Thr Glu Thr Ile  Ala Ala Gln 
    1175                 1180                 1185             


Glu Ser  Ser Glu Arg Val Lys  Pro Tyr Thr Tyr Asp  Phe Ala Pro 
    1190                 1195                 1200             


Val Gly  Ala Thr Phe Val Lys  Val Thr Val Thr Asn  Ala Asp Thr 
    1205                 1210                 1215             


Thr Thr  Pro Ser Gly Val Val  Cys Ala Gly Leu Thr  Glu Ile Glu 
    1220                 1225                 1230             


Leu Lys  Thr Ala Thr Ser Lys  Phe Val Thr Asn Thr  Ser Ala Ala 
    1235                 1240                 1245             


Leu Ser  Ser Leu Thr Val Asn  Gly Thr Lys Val Ser  Asp Ser Val 
    1250                 1255                 1260             


Leu Ala  Ala Gly Ser Tyr Asn  Thr Pro Ala Ile Ile  Ala Asp Val 
    1265                 1270                 1275             


Lys Ala  Glu Gly Glu Gly Asn  Ala Ser Val Thr Val  Leu Pro Ala 
    1280                 1285                 1290             


His Asp  Asn Val Ile Arg Val  Ile Thr Glu Ser Glu  Asp His Val 
    1295                 1300                 1305             


Thr Arg  Lys Thr Phe Thr Ile  Asn Leu Gly Thr Glu  Gln Glu Phe 
    1310                 1315                 1320             


Pro Ala  Asp Ser Asp Glu Arg  Asp 
    1325                 1330     


