               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> GPD- YEAST STRAINS WITH IMPROVED OSMOTOLERANCE

<130> 29315-WO-PCT

<150> EP13167002.8
<151> 2013-05-08

<160> 6

<170> BiSSAP 1.2

<210> 1
<211> 1404
<212> DNA
<213> Escherichia coli

<220> 
<221> source
<222> 1..1404
<223> /organism="Escherichia coli"
      /mol_type="unassigned DNA"

<400> 1
atgaaccaac aagatatcga acaagttgtc aaggctgtct tgttgaaaat gcaatcttct      60

gacactccat ctgctgctgt ccacgaaatg ggtgttttcg cttctttgga cgacgctgtt     120

gctgctgcca aggttgctca acaaggtttg aaatctgttg ccatgagaca attggccatt     180

gctgccatca gagaagctgg tgaaaagcat gccagagact tggctgaatt ggctgtctcc     240

gaaaccggta tgggtagagt tgaagacaaa ttcgctaaga acgttgctca agctagaggt     300

actccaggtg tcgaatgttt gtctccacaa gtcttgaccg gtgataatgg tttgactttg     360

attgaaaatg ctccatgggg tgttgttgct tccgtcaccc catctaccaa cccagctgct     420

actgtcatca acaacgccat ctctttgatt gctgctggta actccgttat cttcgctcca     480

cacccagctg ccaagaaggt ttctcaaaga gccatcactc tattgaacca agccattgtt     540

gctgctggtg gtccagaaaa cttgttggtc actgttgcca acccagatat cgaaactgct     600

caaagattat tcaagttccc aggtatcggt ctattagtcg tcactggtgg tgaagctgtt     660

gttgaagctg ccagaaagca caccaacaag agattgattg ctgctggtgc tggtaaccct     720

cctgttgttg tcgatgaaac cgctgatttg gccagagctg ctcaatccat tgtcaagggt     780

gcttctttcg acaacaacat catctgtgct gacgaaaagg ttttgattgt tgttgactcc     840

gttgctgacg aattgatgag attgatggaa ggtcaacatg ccgtcaagtt gactgctgaa     900

caagctcaac aattgcaacc agttttgttg aagaacatcg atgaaagagg taagggtacc     960

gtctccagag actgggttgg tagagatgct ggtaagattg ctgctgccat cggtttgaag    1020

gttccacaag aaaccagatt attattcgtc gaaaccaccg ctgaacaccc atttgctgtc    1080

actgaattga tgatgccagt cttaccagtt gtccgtgttg ctaacgttgc tgacgctatt    1140

gctttggctg tcaaattgga aggtggttgt caccacactg ctgccatgca ctccagaaac    1200

atcgaaaaca tgaaccaaat ggctaacgcc attgacactt ccatctttgt caagaacggt    1260

ccatgtatcg ctggtttggg tttgggtggt gaaggttgga ccaccatgac catcaccacc    1320

ccaactggtg aaggtgtcac ttctgccaga actttcgtca gattacgtcg ttgtgttttg    1380

gtcgatgctt tcagaattgt ttaa                                           1404


<210> 2
<211> 467
<212> PRT
<213> Escherichia coli


<400> 2
Met Asn Gln Gln Asp Ile Glu Gln Val Val Lys Ala Val Leu Leu Lys 
1               5                   10                  15      
Met Gln Ser Ser Asp Thr Pro Ser Ala Ala Val His Glu Met Gly Val 
            20                  25                  30          
Phe Ala Ser Leu Asp Asp Ala Val Ala Ala Ala Lys Val Ala Gln Gln 
        35                  40                  45              
Gly Leu Lys Ser Val Ala Met Arg Gln Leu Ala Ile Ala Ala Ile Arg 
    50                  55                  60                  
Glu Ala Gly Glu Lys His Ala Arg Asp Leu Ala Glu Leu Ala Val Ser 
65                  70                  75                  80  
Glu Thr Gly Met Gly Arg Val Glu Asp Lys Phe Ala Lys Asn Val Ala 
                85                  90                  95      
Gln Ala Arg Gly Thr Pro Gly Val Glu Cys Leu Ser Pro Gln Val Leu 
            100                 105                 110         
Thr Gly Asp Asn Gly Leu Thr Leu Ile Glu Asn Ala Pro Trp Gly Val 
        115                 120                 125             
Val Ala Ser Val Thr Pro Ser Thr Asn Pro Ala Ala Thr Val Ile Asn 
    130                 135                 140                 
Asn Ala Ile Ser Leu Ile Ala Ala Gly Asn Ser Val Ile Phe Ala Pro 
145                 150                 155                 160 
His Pro Ala Ala Lys Lys Val Ser Gln Arg Ala Ile Thr Leu Leu Asn 
                165                 170                 175     
Gln Ala Ile Val Ala Ala Gly Gly Pro Glu Asn Leu Leu Val Thr Val 
            180                 185                 190         
Ala Asn Pro Asp Ile Glu Thr Ala Gln Arg Leu Phe Lys Phe Pro Gly 
        195                 200                 205             
Ile Gly Leu Leu Val Val Thr Gly Gly Glu Ala Val Val Glu Ala Ala 
    210                 215                 220                 
Arg Lys His Thr Asn Lys Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro 
225                 230                 235                 240 
Pro Val Val Val Asp Glu Thr Ala Asp Leu Ala Arg Ala Ala Gln Ser 
                245                 250                 255     
Ile Val Lys Gly Ala Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu 
            260                 265                 270         
Lys Val Leu Ile Val Val Asp Ser Val Ala Asp Glu Leu Met Arg Leu 
        275                 280                 285             
Met Glu Gly Gln His Ala Val Lys Leu Thr Ala Glu Gln Ala Gln Gln 
    290                 295                 300                 
Leu Gln Pro Val Leu Leu Lys Asn Ile Asp Glu Arg Gly Lys Gly Thr 
305                 310                 315                 320 
Val Ser Arg Asp Trp Val Gly Arg Asp Ala Gly Lys Ile Ala Ala Ala 
                325                 330                 335     
Ile Gly Leu Lys Val Pro Gln Glu Thr Arg Leu Leu Phe Val Glu Thr 
            340                 345                 350         
Thr Ala Glu His Pro Phe Ala Val Thr Glu Leu Met Met Pro Val Leu 
        355                 360                 365             
Pro Val Val Arg Val Ala Asn Val Ala Asp Ala Ile Ala Leu Ala Val 
    370                 375                 380                 
Lys Leu Glu Gly Gly Cys His His Thr Ala Ala Met His Ser Arg Asn 
385                 390                 395                 400 
Ile Glu Asn Met Asn Gln Met Ala Asn Ala Ile Asp Thr Ser Ile Phe 
                405                 410                 415     
Val Lys Asn Gly Pro Cys Ile Ala Gly Leu Gly Leu Gly Gly Glu Gly 
            420                 425                 430         
Trp Thr Thr Met Thr Ile Thr Thr Pro Thr Gly Glu Gly Val Thr Ser 
        435                 440                 445             
Ala Arg Thr Phe Val Arg Leu Arg Arg Cys Val Leu Val Asp Ala Phe 
    450                 455                 460                 
Arg Ile Val 
465         

<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer"
      /mol_type="unassigned DNA"

<400> 3
aaacgggcac aacctcaatg                                                20


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer"
      /mol_type="unassigned DNA"

<400> 4
gtgaccatgt tgacgttcag                                                20


<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer"
      /mol_type="unassigned DNA"

<400> 5
ggcggtgatg gttggcattg                                                20


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer"
      /mol_type="unassigned DNA"

<400> 6
gggagggcgt gaatgtaagc                                                20


