                        
                                         

<110>  Ϻо
 
<120>  GPC-3ǶϿԭ嵰׵ĺἰGPC-3ǶϿԭ嵰׵Tܰ
       ϸ

<130>  GPC3 CART

<160>  32    

<170>  PatentIn version 3.3

<210>  1
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer 1

<400>  1
gatgttgtga tgactcagtc tc                                              22


<210>  2
<211>  34
<212>  DNA
<213>  aritificial

<400>  2
gcgctggcgt cgtggttgag gagacggtga ccag                                 34


<210>  3
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  primer 3

<400>  3
accacgacgc cagcgccgcg accac                                           25


<210>  4
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  primer 4

<400>  4
gaggtcgacc tagcgagggg gcagggcctg catgtgaag                            39


<210>  5
<211>  62
<212>  DNA
<213>  artificial 

<220>
<223>  primer 5

<400>  5
gaggtcgacc tacgcggggg cgtctgcgct cctgctgaac ttcactctgg tgataaccag     60

tg                                                                    62


<210>  6
<211>  52
<212>  DNA
<213>  artificial

<220>
<223>  primer 6

<400>  6
cttacgcgtc ctagcgctac cggtcgccac catggtgagc aagggcgagg ag             52


<210>  7
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  primer 7

<400>  7
cggcctggcg gcgtggagca g                                               21


<210>  8
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer 8

<400>  8
ttttgggtgc tggtggtggt tgg                                             23


<210>  9
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  primer 9

<400>  9
gctgaacttc actctggagc gataggctgc gaag                                 34


<210>  10
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  primer 10

<400>  10
aaacggggca gaaagaaact c                                               21


<210>  11
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  primer 11

<400>  11
cagttcacat cctccttc                                                   18


<210>  12
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  primer 12

<400>  12
cactggttat caccagagtg aagttcagca ggagc                                35


<210>  13
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  primer 13

<400>  13
gaggtcgacc tagcgagggg gcagggcctg catg                                 34


<210>  14
<211>  18
<212>  DNA
<213>  artificial 

<220>
<223>  primer 14

<400>  14
accacgacgc cagcgccg                                                   18


<210>  15
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  primer 15

<400>  15
cacccagaaa ataataaag                                                  19


<210>  16
<211>  52
<212>  DNA
<213>  artificial 

<220>
<223>  primer 16

<400>  16
cttacgcgtc ctagcgctac cggtcgccac catggtgagc aagggcgagg ag             52


<210>  17
<211>  21
<212>  DNA
<213>  artificial 

<220>
<223>  primer 17

<400>  17
cggcctggcg gcgtggagca g                                               21


<210>  18
<211>  1266
<212>  DNA
<213>  artificial

<220>
<223>  GPC3 CAR  (GPC3-Z)

<400>  18
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccctggaga gccggcctcc     60

atctcctgca gatctagtca gagccttgta cacagtaatg ccaacaccta tttacattgg    120

tacctgcaga agccagggca gtctccacag ctcctgatct ataaagtttc caaccgattt    180

tctggggtcc ctgacaggtt cagtggcagt ggatcaggca cagattttac actgaaaatc    240

agcagagtgg aggctgagga tgttggggtt tattactgct ctcaaaatac acatgttcct    300

cctacgtttg gccaggggac caagctggag atcaaacgtg gtggaggcgg ttcaggcgga    360

ggtggctctg gcggtggcgg atcgcaggtg cagctggtgc agtctggagc tgaggtgaag    420

aagcctgggg cctcagtgaa ggtctcctgc aaggcttctg gatacacctt caccgactat    480

gaaatgcact gggtgcgaca ggcccctgga caagggcttg agtggatggg agctcttgat    540

cctaaaactg gtgatactgc ctacagtcag aagttcaagg gcagagtcac gctgaccgcg    600

gacgaatcca cgagcacagc ctacatggag ctgagcagcc tgagatctga ggacacggcc    660

gtgtattact gtacaagatt ctactcctat acttactggg gccagggaac cctggtcacc    720

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg    780

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg    840

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg    900

gtccttctcc tgtcactggt tatcaccaga gtgaagttca gcaggagcgc agacgccccc    960

gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg aagagaggag   1020

tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa gccgcagaga   1080

aggaagaacc ctcaggaagg cctgtacaat gaactgcaga aagataagat ggcggaggcc   1140

tacagtgaga ttgggatgaa aggcgagcgc cggaggggca aggggcacga tggcctttac   1200

cagggtctca gtacagccac caaggacacc tacgacgccc ttcacatgca ggccctgccc   1260

cctcgc                                                              1266


<210>  19
<211>  1392
<212>  DNA
<213>  artificial

<220>
<223>  GPC3-BBZ

<400>  19
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccctggaga gccggcctcc     60

atctcctgca gatctagtca gagccttgta cacagtaatg ccaacaccta tttacattgg    120

tacctgcaga agccagggca gtctccacag ctcctgatct ataaagtttc caaccgattt    180

tctggggtcc ctgacaggtt cagtggcagt ggatcaggca cagattttac actgaaaatc    240

agcagagtgg aggctgagga tgttggggtt tattactgct ctcaaaatac acatgttcct    300

cctacgtttg gccaggggac caagctggag atcaaacgtg gtggaggcgg ttcaggcgga    360

ggtggctctg gcggtggcgg atcgcaggtg cagctggtgc agtctggagc tgaggtgaag    420

aagcctgggg cctcagtgaa ggtctcctgc aaggcttctg gatacacctt caccgactat    480

gaaatgcact gggtgcgaca ggcccctgga caagggcttg agtggatggg agctcttgat    540

cctaaaactg gtgatactgc ctacagtcag aagttcaagg gcagagtcac gctgaccgcg    600

gacgaatcca cgagcacagc ctacatggag ctgagcagcc tgagatctga ggacacggcc    660

gtgtattact gtacaagatt ctactcctat acttactggg gccagggaac cctggtcacc    720

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg    780

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg    840

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg    900

gtccttctcc tgtcactggt tatcaccaaa cggggcagaa agaaactcct gtatatattc    960

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga   1020

tttccagaag aagaagaagg aggatgtgaa ctgagagtga agttcagcag gagcgcagac   1080

gcccccgcgt accagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga   1140

gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg   1200

cagagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg   1260

gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc   1320

ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc   1380

ctgccccctc gc                                                       1392


<210>  20
<211>  1410
<212>  DNA
<213>  artificial

<220>
<223>  GPC3-28Z

<400>  20
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccctggaga gccggcctcc     60

atctcctgca gatctagtca gagccttgta cacagtaatg ccaacaccta tttacattgg    120

tacctgcaga agccagggca gtctccacag ctcctgatct ataaagtttc caaccgattt    180

tctggggtcc ctgacaggtt cagtggcagt ggatcaggca cagattttac actgaaaatc    240

agcagagtgg aggctgagga tgttggggtt tattactgct ctcaaaatac acatgttcct    300

cctacgtttg gccaggggac caagctggag atcaaacgtg gtggaggcgg ttcaggcgga    360

ggtggctctg gcggtggcgg atcgcaggtg cagctggtgc agtctggagc tgaggtgaag    420

aagcctgggg cctcagtgaa ggtctcctgc aaggcttctg gatacacctt caccgactat    480

gaaatgcact gggtgcgaca ggcccctgga caagggcttg agtggatggg agctcttgat    540

cctaaaactg gtgatactgc ctacagtcag aagttcaagg gcagagtcac gctgaccgcg    600

gacgaatcca cgagcacagc ctacatggag ctgagcagcc tgagatctga ggacacggcc    660

gtgtattact gtacaagatt ctactcctat acttactggg gccagggaac cctggtcacc    720

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg    780

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg    840

agggggctgg acttcgcctg tgatttttgg gtgctggtgg tggttggtgg agtcctggct    900

tgctatagct tgctagtaac agtggccttt attattttct gggtgaggag taagaggagc    960

aggctcctgc acagtgacta catgaacatg actccccgcc gccccgggcc aacccgcaag   1020

cattaccagc cctatgcccc accacgcgac ttcgcagcct atcgctccag agtgaagttc   1080

agcaggagcg cagacgcccc cgcgtaccag cagggccaga accagctcta taacgagctc   1140

aatctaggac gaagagagga gtacgatgtt ttggacaaga gacgtggccg ggaccctgag   1200

atggggggaa agccgcagag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag   1260

aaagataaga tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc   1320

aaggggcacg atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc   1380

cttcacatgc aggccctgcc ccctcgctag                                    1410


<210>  21
<211>  1533
<212>  DNA
<213>  artificial

<220>
<223>  GPC3-CAR (GPC3-28BBZ)

<400>  21
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccctggaga gccggcctcc     60

atctcctgca gatctagtca gagccttgta cacagtaatg ccaacaccta tttacattgg    120

tacctgcaga agccagggca gtctccacag ctcctgatct ataaagtttc caaccgattt    180

tctggggtcc ctgacaggtt cagtggcagt ggatcaggca cagattttac actgaaaatc    240

agcagagtgg aggctgagga tgttggggtt tattactgct ctcaaaatac acatgttcct    300

cctacgtttg gccaggggac caagctggag atcaaacgtg gtggaggcgg ttcaggcgga    360

ggtggctctg gcggtggcgg atcgcaggtg cagctggtgc agtctggagc tgaggtgaag    420

aagcctgggg cctcagtgaa ggtctcctgc aaggcttctg gatacacctt caccgactat    480

gaaatgcact gggtgcgaca ggcccctgga caagggcttg agtggatggg agctcttgat    540

cctaaaactg gtgatactgc ctacagtcag aagttcaagg gcagagtcac gctgaccgcg    600

gacgaatcca cgagcacagc ctacatggag ctgagcagcc tgagatctga ggacacggcc    660

gtgtattact gtacaagatt ctactcctat acttactggg gccagggaac cctggtcacc    720

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg    780

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg    840

agggggctgg acttcgcctg tgatttttgg gtgctggtgg tggttggtgg agtcctggct    900

tgctatagct tgctagtaac agtggccttt attattttct gggtgaggag taagaggagc    960

aggctcctgc acagtgacta catgaacatg actccccgcc gccccgggcc aacccgcaag   1020

cattaccagc cctatgcccc accacgcgac ttcgcagcct atcgctccaa acggggcaga   1080

aagaaactcc tgtatatatt caaacaacca tttatgagac cagtacaaac tactcaagag   1140

gaagatggct gtagctgccg atttccagaa gaagaagaag gaggatgtga actgagagtg   1200

aagttcagca ggagcgcaga cgcccccgcg taccagcagg gccagaacca gctctataac   1260

gagctcaatc taggacgaag agaggagtac gatgttttgg acaagagacg tggccgggac   1320

cctgagatgg ggggaaagcc gcagagaagg aagaaccctc aggaaggcct gtacaatgaa   1380

ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg   1440

aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac   1500

gacgcccttc acatgcaggc cctgccccct cgc                                1533


<210>  22
<211>  422
<212>  PRT
<213>  artificial

<220>
<223>  GPC3-CAR(GPC3-Z)

<400>  22

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Ala Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
    130                 135                 140                 


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
145                 150                 155                 160 


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
            180                 185                 190         


Lys Gly Arg Val Thr Leu Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
        195                 200                 205             


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
                245                 250                 255     


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            260                 265                 270         


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        275                 280                 285             


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
    290                 295                 300                 


Ser Leu Val Ile Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
305                 310                 315                 320 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
                325                 330                 335     


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
            340                 345                 350         


Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu 
        355                 360                 365             


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
    370                 375                 380                 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
385                 390                 395                 400 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
                405                 410                 415     


Gln Ala Leu Pro Pro Arg 
            420         


<210>  23
<211>  464
<212>  PRT
<213>  artificial

<220>
<223>  GPC3-BBZ amino acid

<400>  23

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Ala Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
    130                 135                 140                 


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
145                 150                 155                 160 


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
            180                 185                 190         


Lys Gly Arg Val Thr Leu Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
        195                 200                 205             


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
                245                 250                 255     


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            260                 265                 270         


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        275                 280                 285             


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
    290                 295                 300                 


Ser Leu Val Ile Thr Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
305                 310                 315                 320 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                325                 330                 335     


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
            340                 345                 350         


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
        355                 360                 365             


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    370                 375                 380                 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
385                 390                 395                 400 


Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
                405                 410                 415     


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
            420                 425                 430         


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
        435                 440                 445             


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460                 


<210>  24
<211>  469
<212>  PRT
<213>  artificial

<220>
<223>  GPC3-28Z

<400>  24

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Ala Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
    130                 135                 140                 


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
145                 150                 155                 160 


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
            180                 185                 190         


Lys Gly Arg Val Thr Leu Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
        195                 200                 205             


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
                245                 250                 255     


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            260                 265                 270         


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        275                 280                 285             


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
    290                 295                 300                 


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
305                 310                 315                 320 


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
                325                 330                 335     


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
            340                 345                 350         


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
        355                 360                 365             


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
    370                 375                 380                 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
385                 390                 395                 400 


Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
                405                 410                 415     


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
            420                 425                 430         


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
        435                 440                 445             


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
    450                 455                 460                 


Ala Leu Pro Pro Arg 
465                 


<210>  25
<211>  511
<212>  PRT
<213>  artificial

<220>
<223>  GPC3-CAR (GPC3-Z8BBZ)

<400>  25

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Ala Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
    130                 135                 140                 


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
145                 150                 155                 160 


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
            180                 185                 190         


Lys Gly Arg Val Thr Leu Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
        195                 200                 205             


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
                245                 250                 255     


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            260                 265                 270         


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        275                 280                 285             


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
    290                 295                 300                 


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
305                 310                 315                 320 


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
                325                 330                 335     


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
            340                 345                 350         


Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys 
        355                 360                 365             


Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys 
    370                 375                 380                 


Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val 
385                 390                 395                 400 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
                405                 410                 415     


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
            420                 425                 430         


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln 
        435                 440                 445             


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
    450                 455                 460                 


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
465                 470                 475                 480 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
                485                 490                 495     


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            500                 505                 510     


<210>  26
<211>  10487
<212>  DNA
<213>  artificial

<220>
<223>  pWPT-eGFP-F2A-GPC3-delta Z


<220>
<221>  MluI
<222>  (2448)..(2453)

<220>
<221>  ORF
<222>  (2476)..(4290)

<220>
<221>  SalI
<222>  (4294)..(4299)

<400>  26
ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac     60

acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca    120

ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc    180

aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg    240

gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga    300

gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc    360

gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga    420

tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg    480

agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc    540

ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct    600

cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa    660

gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg    720

gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag    780

aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg    840

gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg    900

gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc    960

tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga   1020

tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac   1080

accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag   1140

caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa   1200

gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg   1260

caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg   1320

ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg   1380

ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg   1440

cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc   1500

tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa   1560

aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac   1620

agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct   1680

taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat   1740

tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt   1800

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg   1860

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc   1920

tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag   1980

acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa   2040

gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag   2100

acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga   2160

gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat   2220

cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa   2280

ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg   2340

gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt   2400

ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc taagcttacg cgtcctagcg   2460

ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc   2520

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag   2580

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc   2640

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac   2700

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag   2760

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc   2820

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc   2880

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc   2940

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc   3000

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg   3060

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag   3120

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac   3180

gagctgtaca agtccggagt gaaacagact ttgaattttg accttctgaa gttggcagga   3240

gacgttgagt ccaaccctgg gcccatggcc ttaccagtga ccgccttgct cctgccgctg   3300

gccttgctgc tccacgccgc caggccggat gttgtgatga ctcagtctcc actctccctg   3360

cccgtcaccc ctggagagcc ggcctccatc tcctgcagat ctagtcagag ccttgtacac   3420

agtaatgcca acacctattt acattggtac ctgcagaagc cagggcagtc tccacagctc   3480

ctgatctata aagtttccaa ccgattttct ggggtccctg acaggttcag tggcagtgga   3540

tcaggcacag attttacact gaaaatcagc agagtggagg ctgaggatgt tggggtttat   3600

tactgctctc aaaatacaca tgttcctcct acgtttggcc aggggaccaa gctggagatc   3660

aaacgtggtg gaggcggttc aggcggaggt ggctctggcg gtggcggatc gcaggtgcag   3720

ctggtgcagt ctggagctga ggtgaagaag cctggggcct cagtgaaggt ctcctgcaag   3780

gcttctggat acaccttcac cgactatgaa atgcactggg tgcgacaggc ccctggacaa   3840

gggcttgagt ggatgggagc tcttgatcct aaaactggtg atactgccta cagtcagaag   3900

ttcaagggca gagtcacgct gaccgcggac gaatccacga gcacagccta catggagctg   3960

agcagcctga gatctgagga cacggccgtg tattactgta caagattcta ctcctatact   4020

tactggggcc agggaaccct ggtcaccgtc tcctcaacca cgacgccagc gccgcgacca   4080

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg   4140

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatctacatc   4200

tgggcgccct tggccgggac ttgtggggtc cttctcctgt cactggttat caccagagtg   4260

aagttcagca ggagcgcaga cgcccccgcg taggtcgacc tcgagggaat tccgataatc   4320

aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt   4380

ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg   4440

ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc   4500

ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt   4560

ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg   4620

ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg   4680

gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct   4740

gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc   4800

cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc   4860

ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcgagc   4920

tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa   4980

gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatgg gatcaattca   5040

ccatgggaat aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac   5100

tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc   5160

actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt   5220

gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag   5280

cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata   5340

cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt   5400

gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc   5460

ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc   5520

agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc   5580

ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg   5640

gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca   5700

ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg   5760

gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag   5820

ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca   5880

gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc   5940

cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc   6000

atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt   6060

catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac   6120

ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc   6180

ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt   6240

cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct   6300

ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga   6360

tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag   6420

cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca   6480

actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga   6540

aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag   6600

tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc   6660

ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa   6720

tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt   6780

gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg   6840

gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt   6900

tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg   6960

gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat   7020

ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact   7080

gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa   7140

aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt   7200

ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt   7260

ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg   7320

tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca   7380

gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt   7440

agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga   7500

taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc   7560

gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact   7620

gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga   7680

caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg   7740

aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt   7800

tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt   7860

acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga   7920

ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac   7980

gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc   8040

tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt   8100

ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca   8160

gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat   8220

ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg   8280

cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc   8340

gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta   8400

ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact   8460

ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt   8520

ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta   8580

agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga   8640

catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa   8700

aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg   8760

ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag   8820

cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg   8880

gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc   8940

accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg   9000

caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc   9060

ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg   9120

gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag   9180

cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt   9240

taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg   9300

gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct   9360

cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga   9420

tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga   9480

aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct   9540

ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt   9600

gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta   9660

atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg   9720

actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc   9780

cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct   9840

ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat   9900

attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta   9960

ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta  10020

gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag  10080

atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac  10140

ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca  10200

gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt  10260

tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc  10320

aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc  10380

actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc  10440

attttaaaga aattgtattt gttaaatatg tactacaaac ttagtag                10487


<210>  27
<211>  10790
<212>  DNA
<213>  artificial

<220>
<223>  pWPT-eGFP-F2A-GPC3-Z


<220>
<221>  MluI
<222>  (2448)..(2453)

<220>
<221>  ORF
<222>  (2476)..(4593)

<220>
<221>  SalI
<222>  (4597)..(4602)

<400>  27
ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac     60

acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca    120

ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc    180

aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg    240

gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga    300

gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc    360

gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga    420

tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg    480

agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc    540

ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct    600

cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa    660

gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg    720

gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag    780

aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg    840

gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg    900

gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc    960

tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga   1020

tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac   1080

accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag   1140

caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa   1200

gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg   1260

caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg   1320

ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg   1380

ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg   1440

cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc   1500

tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa   1560

aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac   1620

agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct   1680

taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat   1740

tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt   1800

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg   1860

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc   1920

tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag   1980

acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa   2040

gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag   2100

acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga   2160

gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat   2220

cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa   2280

ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg   2340

gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt   2400

ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc taagcttacg cgtcctagcg   2460

ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc   2520

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag   2580

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc   2640

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac   2700

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag   2760

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc   2820

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc   2880

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc   2940

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc   3000

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg   3060

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag   3120

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac   3180

gagctgtaca agtccggagt gaaacagact ttgaattttg accttctgaa gttggcagga   3240

gacgttgagt ccaaccctgg gcccatggcc ttaccagtga ccgccttgct cctgccgctg   3300

gccttgctgc tccacgccgc caggccggat gttgtgatga ctcagtctcc actctccctg   3360

cccgtcaccc ctggagagcc ggcctccatc tcctgcagat ctagtcagag ccttgtacac   3420

agtaatgcca acacctattt acattggtac ctgcagaagc cagggcagtc tccacagctc   3480

ctgatctata aagtttccaa ccgattttct ggggtccctg acaggttcag tggcagtgga   3540

tcaggcacag attttacact gaaaatcagc agagtggagg ctgaggatgt tggggtttat   3600

tactgctctc aaaatacaca tgttcctcct acgtttggcc aggggaccaa gctggagatc   3660

aaacgtggtg gaggcggttc aggcggaggt ggctctggcg gtggcggatc gcaggtgcag   3720

ctggtgcagt ctggagctga ggtgaagaag cctggggcct cagtgaaggt ctcctgcaag   3780

gcttctggat acaccttcac cgactatgaa atgcactggg tgcgacaggc ccctggacaa   3840

gggcttgagt ggatgggagc tcttgatcct aaaactggtg atactgccta cagtcagaag   3900

ttcaagggca gagtcacgct gaccgcggac gaatccacga gcacagccta catggagctg   3960

agcagcctga gatctgagga cacggccgtg tattactgta caagattcta ctcctatact   4020

tactggggcc agggaaccct ggtcaccgtc tcctcaacca cgacgccagc gccgcgacca   4080

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg   4140

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatctacatc   4200

tgggcgccct tggccgggac ttgtggggtc cttctcctgt cactggttat caccagagtg   4260

aagttcagca ggagcgcaga cgcccccgcg taccagcagg gccagaacca gctctataac   4320

gagctcaatc taggacgaag agaggagtac gatgttttgg acaagagacg tggccgggac   4380

cctgagatgg ggggaaagcc gcagagaagg aagaaccctc aggaaggcct gtacaatgaa   4440

ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg   4500

aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac   4560

gacgcccttc acatgcaggc cctgccccct cgctaggtcg acctcgaggg aattccgata   4620

atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc   4680

cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta   4740

tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt   4800

ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg   4860

gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta   4920

ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt   4980

tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg   5040

cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca   5100

atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc   5160

gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcg   5220

agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta   5280

aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat   5340

tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc tttttgcttg   5400

tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa   5460

cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct   5520

gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc   5580

tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg   5640

atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat   5700

tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga   5760

gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg   5820

gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg   5880

gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat   5940

ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg   6000

gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg   6060

gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat   6120

aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag   6180

ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc   6240

atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc   6300

gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa   6360

tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg   6420

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata   6480

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg   6540

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac   6600

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact   6660

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat   6720

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga   6780

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac   6840

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat   6900

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac   6960

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct   7020

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac   7080

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga   7140

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg   7200

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact   7260

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac   7320

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta   7380

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt   7440

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga   7500

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc   7560

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt   7620

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc   7680

gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc   7740

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg   7800

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg   7860

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga   7920

actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc   7980

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg   8040

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg   8100

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt   8160

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc   8220

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg   8280

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc   8340

gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg   8400

tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag   8460

tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg   8520

catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact   8580

ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag   8640

gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc   8700

ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc   8760

actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact   8820

ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt   8880

ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg   8940

ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg   9000

gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa   9060

ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc   9120

gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac   9180

ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt   9240

gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc   9300

ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc   9360

tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg   9420

cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct   9480

gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg   9540

ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca   9600

ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat   9660

cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata   9720

cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg   9780

tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa   9840

gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg   9900

tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct   9960

ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg  10020

ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact  10080

ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt  10140

gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa  10200

aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc  10260

ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct  10320

ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta  10380

gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca  10440

cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt  10500

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt  10560

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg  10620

atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt  10680

accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt  10740

ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag             10790


<210>  28
<211>  10916
<212>  DNA
<213>  artificial

<220>
<223>  pWPT-eGFP-F2A-GPC3-BBZ


<220>
<221>  MluI
<222>  (2448)..(2453)

<220>
<221>  ORF
<222>  (2476)..(4719)

<220>
<221>  SalI
<222>  (4723)..(4728)

<400>  28
ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac     60

acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca    120

ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc    180

aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg    240

gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga    300

gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc    360

gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga    420

tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg    480

agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc    540

ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct    600

cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa    660

gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg    720

gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag    780

aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg    840

gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg    900

gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc    960

tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga   1020

tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac   1080

accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag   1140

caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa   1200

gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg   1260

caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg   1320

ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg   1380

ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg   1440

cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc   1500

tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa   1560

aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac   1620

agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct   1680

taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat   1740

tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt   1800

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg   1860

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc   1920

tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag   1980

acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa   2040

gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag   2100

acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga   2160

gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat   2220

cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa   2280

ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg   2340

gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt   2400

ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc taagcttacg cgtcctagcg   2460

ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc   2520

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag   2580

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc   2640

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac   2700

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag   2760

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc   2820

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc   2880

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc   2940

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc   3000

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg   3060

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag   3120

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac   3180

gagctgtaca agtccggagt gaaacagact ttgaattttg accttctgaa gttggcagga   3240

gacgttgagt ccaaccctgg gcccatggcc ttaccagtga ccgccttgct cctgccgctg   3300

gccttgctgc tccacgccgc caggccggat gttgtgatga ctcagtctcc actctccctg   3360

cccgtcaccc ctggagagcc ggcctccatc tcctgcagat ctagtcagag ccttgtacac   3420

agtaatgcca acacctattt acattggtac ctgcagaagc cagggcagtc tccacagctc   3480

ctgatctata aagtttccaa ccgattttct ggggtccctg acaggttcag tggcagtgga   3540

tcaggcacag attttacact gaaaatcagc agagtggagg ctgaggatgt tggggtttat   3600

tactgctctc aaaatacaca tgttcctcct acgtttggcc aggggaccaa gctggagatc   3660

aaacgtggtg gaggcggttc aggcggaggt ggctctggcg gtggcggatc gcaggtgcag   3720

ctggtgcagt ctggagctga ggtgaagaag cctggggcct cagtgaaggt ctcctgcaag   3780

gcttctggat acaccttcac cgactatgaa atgcactggg tgcgacaggc ccctggacaa   3840

gggcttgagt ggatgggagc tcttgatcct aaaactggtg atactgccta cagtcagaag   3900

ttcaagggca gagtcacgct gaccgcggac gaatccacga gcacagccta catggagctg   3960

agcagcctga gatctgagga cacggccgtg tattactgta caagattcta ctcctatact   4020

tactggggcc agggaaccct ggtcaccgtc tcctcaacca cgacgccagc gccgcgacca   4080

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg   4140

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatctacatc   4200

tgggcgccct tggccgggac ttgtggggtc cttctcctgt cactggttat caccaaacgg   4260

ggcagaaaga aactcctgta tatattcaaa caaccattta tgagaccagt acaaactact   4320

caagaggaag atggctgtag ctgccgattt ccagaagaag aagaaggagg atgtgaactg   4380

agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc   4440

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc   4500

cgggaccctg agatgggggg aaagccgcag agaaggaaga accctcagga aggcctgtac   4560

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag   4620

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac   4680

acctacgacg cccttcacat gcaggccctg ccccctcgct aggtcgacct cgagggaatt   4740

ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg   4800

ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt   4860

cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg   4920

agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc   4980

ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc   5040

tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc   5100

ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc   5160

tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg   5220

ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc   5280

gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg   5340

aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac   5400

tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatggg   5460

atcaattcac catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt   5520

tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact   5580

agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc   5640

ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa   5700

aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg   5760

tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag   5820

cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc   5880

gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg   5940

gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc   6000

atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc   6060

accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt   6120

atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc   6180

cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag   6240

tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata   6300

gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct   6360

cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt   6420

ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata   6480

ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt   6540

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag   6600

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca   6660

tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc   6720

agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat   6780

cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc   6840

aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg   6900

gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc   6960

agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat   7020

aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga   7080

gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc   7140

ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc   7200

aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt   7260

aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc   7320

tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc   7380

agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca   7440

ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca   7500

ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt   7560

ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta   7620

acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg   7680

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc   7740

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag   7800

cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa   7860

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc   7920

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc   7980

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta   8040

caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag   8100

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct   8160

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga   8220

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc   8280

ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt   8340

atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg   8400

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg   8460

caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag   8520

ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc   8580

aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa   8640

agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc   8700

ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat   8760

gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt   8820

ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag   8880

aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga   8940

aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa   9000

cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt   9060

cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga   9120

acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg   9180

cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac   9240

cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt   9300

gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg   9360

cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt   9420

gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg   9480

ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac   9540

ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc   9600

aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt   9660

ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat   9720

ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg   9780

cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga   9840

tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga   9900

tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga   9960

tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc  10020

taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga  10080

atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg  10140

ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca  10200

aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc  10260

ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta  10320

tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt  10380

tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt  10440

gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct  10500

tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac  10560

ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg  10620

tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa  10680

gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat  10740

gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac  10800

cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga  10860

attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtag      10916


<210>  29
<211>  10931
<212>  DNA
<213>  artificial

<220>
<223>  pWPT-eGFP-F2A-GPC3-28Z


<220>
<221>  MluI
<222>  (2448)..(2453)

<220>
<221>  ORF
<222>  (2476)..(4734)

<220>
<221>  SalI
<222>  (4738)..(4743)

<400>  29
ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac     60

acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca    120

ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc    180

aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg    240

gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga    300

gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc    360

gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga    420

tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg    480

agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc    540

ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct    600

cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa    660

gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg    720

gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag    780

aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg    840

gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg    900

gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc    960

tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga   1020

tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac   1080

accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag   1140

caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa   1200

gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg   1260

caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg   1320

ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg   1380

ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg   1440

cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc   1500

tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa   1560

aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac   1620

agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct   1680

taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat   1740

tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt   1800

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg   1860

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc   1920

tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag   1980

acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa   2040

gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag   2100

acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga   2160

gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat   2220

cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa   2280

ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg   2340

gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt   2400

ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc taagcttacg cgtcctagcg   2460

ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc   2520

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag   2580

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc   2640

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac   2700

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag   2760

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc   2820

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc   2880

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc   2940

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc   3000

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg   3060

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag   3120

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac   3180

gagctgtaca agtccggagt gaaacagact ttgaattttg accttctgaa gttggcagga   3240

gacgttgagt ccaaccctgg gcccatggcc ttaccagtga ccgccttgct cctgccgctg   3300

gccttgctgc tccacgccgc caggccggat gttgtgatga ctcagtctcc actctccctg   3360

cccgtcaccc ctggagagcc ggcctccatc tcctgcagat ctagtcagag ccttgtacac   3420

agtaatgcca acacctattt acattggtac ctgcagaagc cagggcagtc tccacagctc   3480

ctgatctata aagtttccaa ccgattttct ggggtccctg acaggttcag tggcagtgga   3540

tcaggcacag attttacact gaaaatcagc agagtggagg ctgaggatgt tggggtttat   3600

tactgctctc aaaatacaca tgttcctcct acgtttggcc aggggaccaa gctggagatc   3660

aaacgtggtg gaggcggttc aggcggaggt ggctctggcg gtggcggatc gcaggtgcag   3720

ctggtgcagt ctggagctga ggtgaagaag cctggggcct cagtgaaggt ctcctgcaag   3780

gcttctggat acaccttcac cgactatgaa atgcactggg tgcgacaggc ccctggacaa   3840

gggcttgagt ggatgggagc tcttgatcct aaaactggtg atactgccta cagtcagaag   3900

ttcaagggca gagtcacgct gaccgcggac gaatccacga gcacagccta catggagctg   3960

agcagcctga gatctgagga cacggccgtg tattactgta caagattcta ctcctatact   4020

tactggggcc agggaaccct ggtcaccgtc tcctcaacca cgacgccagc gccgcgacca   4080

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg   4140

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tttttgggtg   4200

ctggtggtgg ttggtggagt cctggcttgc tatagcttgc tagtaacagt ggcctttatt   4260

attttctggg tgaggagtaa gaggagcagg ctcctgcaca gtgactacat gaacatgact   4320

ccccgccgcc ccgggccaac ccgcaagcat taccagccct atgccccacc acgcgacttc   4380

gcagcctatc gctccagagt gaagttcagc aggagcgcag acgcccccgc gtaccagcag   4440

ggccagaacc agctctataa cgagctcaat ctaggacgaa gagaggagta cgatgttttg   4500

gacaagagac gtggccggga ccctgagatg gggggaaagc cgcagagaag gaagaaccct   4560

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt   4620

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt   4680

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgccccc tcgctaggtc   4740

gacctcgagg gaattccgat aatcaacctc tggattacaa aatttgtgaa agattgactg   4800

gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt   4860

atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc   4920

tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt   4980

ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga   5040

ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct   5100

gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga   5160

cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct   5220

gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc   5280

tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg   5340

cctccccgca tcgggaattc gagctcggta cctttaagac caatgactta caaggcagct   5400

gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa   5460

cgaagacaag atgggatcaa ttcaccatgg gaataacttc gtatagcata cattatacga   5520

agttatgctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga   5580

gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct   5640

tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt   5700

ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt   5760

cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac   5820

gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct   5880

ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac   5940

gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc   6000

ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc   6060

tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct   6120

tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc   6180

ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc   6240

ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg   6300

ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg   6360

acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg   6420

catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat   6480

acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac   6540

ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat   6600

gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag   6660

tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc   6720

tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc   6780

acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc   6840

cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc   6900

ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt   6960

ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt   7020

atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat   7080

cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct   7140

tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat   7200

gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc   7260

ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg   7320

ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc   7380

tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta   7440

cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc   7500

ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga   7560

tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat   7620

gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat   7680

caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa   7740

accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa   7800

ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt   7860

aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt   7920

accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata   7980

gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt   8040

ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac   8100

gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga   8160

gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg   8220

ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa   8280

aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat   8340

gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc   8400

tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga   8460

agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg   8520

tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg   8580

caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca   8640

ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact   8700

ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta   8760

atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag   8820

tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg   8880

cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga   8940

cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc   9000

tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag   9060

cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg   9120

actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg   9180

ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc   9240

tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa   9300

ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa   9360

atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt   9420

gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc   9480

gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc   9540

gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc   9600

atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga   9660

aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct   9720

tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg   9780

cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga   9840

acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta   9900

tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca   9960

aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg  10020

ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat  10080

gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat  10140

ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa  10200

aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt  10260

ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc  10320

tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata  10380

atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact  10440

atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt  10500

tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta  10560

cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt  10620

gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca  10680

aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc  10740

aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa  10800

cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt tactctaaac  10860

ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac  10920

aaacttagta g                                                       10931


<210>  30
<211>  11057
<212>  DNA
<213>  artificial

<220>
<223>  pWPT-eGFP-F2A-GPC3-28BBZ


<220>
<221>  MulI
<222>  (2448)..(2453)

<220>
<221>  ORF
<222>  (2476)..(4860)

<220>
<221>  SalI
<222>  (4864)..(4869)

<400>  30
ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac     60

acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca    120

ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc    180

aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg    240

gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga    300

gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc    360

gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga    420

tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg    480

agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc    540

ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct    600

cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa    660

gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg    720

gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag    780

aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg    840

gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg    900

gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc    960

tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga   1020

tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac   1080

accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag   1140

caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa   1200

gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg   1260

caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg   1320

ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg   1380

ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg   1440

cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc   1500

tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa   1560

aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac   1620

agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct   1680

taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat   1740

tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt   1800

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg   1860

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc   1920

tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag   1980

acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa   2040

gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag   2100

acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga   2160

gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat   2220

cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa   2280

ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg   2340

gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt   2400

ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc taagcttacg cgtcctagcg   2460

ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc   2520

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag   2580

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc   2640

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac   2700

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag   2760

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc   2820

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc   2880

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc   2940

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc   3000

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg   3060

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag   3120

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac   3180

gagctgtaca agtccggagt gaaacagact ttgaattttg accttctgaa gttggcagga   3240

gacgttgagt ccaaccctgg gcccatggcc ttaccagtga ccgccttgct cctgccgctg   3300

gccttgctgc tccacgccgc caggccggat gttgtgatga ctcagtctcc actctccctg   3360

cccgtcaccc ctggagagcc ggcctccatc tcctgcagat ctagtcagag ccttgtacac   3420

agtaatgcca acacctattt acattggtac ctgcagaagc cagggcagtc tccacagctc   3480

ctgatctata aagtttccaa ccgattttct ggggtccctg acaggttcag tggcagtgga   3540

tcaggcacag attttacact gaaaatcagc agagtggagg ctgaggatgt tggggtttat   3600

tactgctctc aaaatacaca tgttcctcct acgtttggcc aggggaccaa gctggagatc   3660

aaacgtggtg gaggcggttc aggcggaggt ggctctggcg gtggcggatc gcaggtgcag   3720

ctggtgcagt ctggagctga ggtgaagaag cctggggcct cagtgaaggt ctcctgcaag   3780

gcttctggat acaccttcac cgactatgaa atgcactggg tgcgacaggc ccctggacaa   3840

gggcttgagt ggatgggagc tcttgatcct aaaactggtg atactgccta cagtcagaag   3900

ttcaagggca gagtcacgct gaccgcggac gaatccacga gcacagccta catggagctg   3960

agcagcctga gatctgagga cacggccgtg tattactgta caagattcta ctcctatact   4020

tactggggcc agggaaccct ggtcaccgtc tcctcaacca cgacgccagc gccgcgacca   4080

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg   4140

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tttttgggtg   4200

ctggtggtgg ttggtggagt cctggcttgc tatagcttgc tagtaacagt ggcctttatt   4260

attttctggg tgaggagtaa gaggagcagg ctcctgcaca gtgactacat gaacatgact   4320

ccccgccgcc ccgggccaac ccgcaagcat taccagccct atgccccacc acgcgacttc   4380

gcagcctatc gctccaaacg gggcagaaag aaactcctgt atatattcaa acaaccattt   4440

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa   4500

gaagaaggag gatgtgaact gagagtgaag ttcagcagga gcgcagacgc ccccgcgtac   4560

cagcagggcc agaaccagct ctataacgag ctcaatctag gacgaagaga ggagtacgat   4620

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgca gagaaggaag   4680

aaccctcagg aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt   4740

gagattggga tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt   4800

ctcagtacag ccaccaagga cacctacgac gcccttcaca tgcaggccct gccccctcgc   4860

taggtcgacc tcgagggaat tccgataatc aacctctgga ttacaaaatt tgtgaaagat   4920

tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc   4980

ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct   5040

ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca   5100

ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt   5160

ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg   5220

cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga   5280

agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt   5340

ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc   5400

cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt   5460

gggccgcctc cccgcatcgg gaattcgagc tcggtacctt taagaccaat gacttacaag   5520

gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac   5580

tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt   5640

atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc   5700

ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg   5760

agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag   5820

acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta   5880

tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt   5940

tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta   6000

tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct   6060

ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc   6120

cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct   6180

ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg   6240

agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc   6300

atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg   6360

ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac   6420

aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc   6480

gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg   6540

gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct   6600

cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg   6660

tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc   6720

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag   6780

gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg   6840

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt   6900

gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt   6960

tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt   7020

attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa   7080

tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag   7140

agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac   7200

aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac   7260

tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac   7320

cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac   7380

tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact   7440

tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg   7500

tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt   7560

tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat   7620

aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta   7680

gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa   7740

tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga   7800

aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac   7860

aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt   7920

tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc   7980

gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat   8040

cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag   8100

acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc   8160

cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag   8220

cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac   8280

aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg   8340

gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct   8400

atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc   8460

tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga   8520

gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga   8580

agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg   8640

cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga   8700

agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc   8760

ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc   8820

ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc   8880

tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag   8940

aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca   9000

ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta   9060

aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc   9120

gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca   9180

catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc   9240

aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct   9300

gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt   9360

tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat   9420

ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt   9480

cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg   9540

ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat   9600

atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg   9660

ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga   9720

ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca   9780

tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc   9840

ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat   9900

ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga   9960

tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg  10020

gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat  10080

tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat  10140

aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg  10200

atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa  10260

tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga  10320

agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg  10380

ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg  10440

cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca  10500

gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta  10560

ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg  10620

aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag  10680

gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat  10740

gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc  10800

atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa  10860

ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat  10920

cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact  10980

ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg  11040

tactacaaac ttagtag                                                 11057


<210>  31
<211>  1815
<212>  DNA
<213>  artificial

<220>
<223>  eGFP-GPC-delta Z

<400>  31
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac     60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac    120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc    180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag    240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc    300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg    360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac    420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac    480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc    540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac    600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc    660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc    720

ggagtgaaac agactttgaa ttttgacctt ctgaagttgg caggagacgt tgagtccaac    780

cctgggccca tggccttacc agtgaccgcc ttgctcctgc cgctggcctt gctgctccac    840

gccgccaggc cggatgttgt gatgactcag tctccactct ccctgcccgt cacccctgga    900

gagccggcct ccatctcctg cagatctagt cagagccttg tacacagtaa tgccaacacc    960

tatttacatt ggtacctgca gaagccaggg cagtctccac agctcctgat ctataaagtt   1020

tccaaccgat tttctggggt ccctgacagg ttcagtggca gtggatcagg cacagatttt   1080

acactgaaaa tcagcagagt ggaggctgag gatgttgggg tttattactg ctctcaaaat   1140

acacatgttc ctcctacgtt tggccagggg accaagctgg agatcaaacg tggtggaggc   1200

ggttcaggcg gaggtggctc tggcggtggc ggatcgcagg tgcagctggt gcagtctgga   1260

gctgaggtga agaagcctgg ggcctcagtg aaggtctcct gcaaggcttc tggatacacc   1320

ttcaccgact atgaaatgca ctgggtgcga caggcccctg gacaagggct tgagtggatg   1380

ggagctcttg atcctaaaac tggtgatact gcctacagtc agaagttcaa gggcagagtc   1440

acgctgaccg cggacgaatc cacgagcaca gcctacatgg agctgagcag cctgagatct   1500

gaggacacgg ccgtgtatta ctgtacaaga ttctactcct atacttactg gggccaggga   1560

accctggtca ccgtctcctc aaccacgacg ccagcgccgc gaccaccaac accggcgccc   1620

accatcgcgt cgcagcccct gtccctgcgc ccagaggcgt gccggccagc ggcggggggc   1680

gcagtgcaca cgagggggct ggacttcgcc tgtgatatct acatctgggc gcccttggcc   1740

gggacttgtg gggtccttct cctgtcactg gttatcacca gagtgaagtt cagcaggagc   1800

gcagacgccc ccgcg                                                    1815


<210>  32
<211>  321
<212>  PRT
<213>  artificial

<220>
<223>  GPC3-deltaZ protein

<400>  32

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Ala Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
    130                 135                 140                 


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
145                 150                 155                 160 


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
            180                 185                 190         


Lys Gly Arg Val Thr Leu Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
        195                 200                 205             


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
                245                 250                 255     


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            260                 265                 270         


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        275                 280                 285             


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
    290                 295                 300                 


Ser Leu Val Ile Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
305                 310                 315                 320 


Ala 
    


