                         SEQUENCE LISTING

<110>  Colorado State University Research Foundation
 
<120>  Sensors and Assays for Ubiquitin or Ubiquitin-like proteins

<130>  CSUV-007/0WUS

<150>  US 61/817,517
<151>  2013-04-30

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  208
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tIVR -  chimeric polypeptide comprising three binding domains 
       that bind ubiquitin in non-overlapping regions

<400>  1

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Gln Glu Val Gln Ala Trp Asp Gly Glu Val 
            20                  25                  30          


Arg Gln Val Ser Lys His Ala Phe Ser Leu Lys Gln Leu Asp Asn Pro 
        35                  40                  45              


Ala Arg Ile Pro Pro Ser Gly Trp Lys Cys Ser Lys Cys Asp Met Arg 
    50                  55                  60                  


Glu Asn Leu Trp Leu Asn Leu Thr Asp Gly Ser Ile Leu Cys Gly Arg 
65                  70                  75                  80  


Arg Tyr Phe Asp Gly Ser Gly Gly Asn Asn His Ala Val Glu His Tyr 
                85                  90                  95      


Arg Glu Thr Gly Tyr Pro Leu Ala Val Lys Leu Gly Thr Ile Thr Pro 
            100                 105                 110         


Asp Gly Ala Asp Val Tyr Ser Tyr Asp Glu Asp Asp Met Val Leu Asp 
        115                 120                 125             


Pro Ser Leu Ala Glu His Leu Ser His Phe Gly Ile Asp Met Leu Lys 
    130                 135                 140                 


Met Gln Lys Gly Ser Ala Ala Ala Glu Glu Ala Glu Leu Asp Leu Lys 
145                 150                 155                 160 


Ala Ala Ile Gln Glu Ser Leu Arg Glu Ala Gly Gly Gly Ser Asp Leu 
                165                 170                 175     


Leu Cys Lys Lys Gly Cys Gly Tyr Tyr Gly Asn Pro Ala Trp Gln Gly 
            180                 185                 190         


Phe Cys Ser Lys Cys Trp Arg Glu Glu Tyr His Lys Ala Arg Gln Lys 
        195                 200                 205             


<210>  2
<211>  209
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tIVR tagged with a fluorophore - chimeric polypeptide comprising 
       three binding domains that bind ubiquitin in non-overlapping 
       regions

<400>  2

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Gln Glu Val Gln Ala Trp Asp Gly Glu Val 
            20                  25                  30          


Arg Gln Val Ser Lys His Ala Phe Ser Leu Lys Gln Leu Asp Asn Pro 
        35                  40                  45              


Ala Arg Ile Pro Pro Ser Gly Trp Lys Cys Ser Lys Cys Asp Met Arg 
    50                  55                  60                  


Glu Asn Leu Trp Leu Asn Leu Thr Asp Gly Ser Ile Leu Cys Gly Arg 
65                  70                  75                  80  


Arg Tyr Phe Asp Gly Ser Gly Gly Asn Asn His Ala Val Glu His Tyr 
                85                  90                  95      


Arg Glu Thr Gly Tyr Pro Leu Ala Val Lys Leu Gly Thr Ile Thr Pro 
            100                 105                 110         


Asp Gly Ala Asp Val Tyr Ser Tyr Asp Glu Asp Asp Met Val Leu Asp 
        115                 120                 125             


Pro Ser Leu Ala Glu His Leu Ser His Phe Gly Ile Asp Met Leu Lys 
    130                 135                 140                 


Met Gln Lys Gly Ser Cys Ala Ala Ala Glu Glu Ala Glu Leu Asp Leu 
145                 150                 155                 160 


Lys Ala Ala Ile Gln Glu Ser Leu Arg Glu Ala Gly Gly Gly Ser Asp 
                165                 170                 175     


Leu Leu Cys Lys Lys Gly Cys Gly Tyr Tyr Gly Asn Pro Ala Trp Gln 
            180                 185                 190         


Gly Phe Cys Ser Lys Cys Trp Arg Glu Glu Tyr His Lys Ala Arg Gln 
        195                 200                 205             


Lys 
    


<210>  3
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tIVR tagged with a fluorophore - chimeric polypeptide comprising 
       three binding domains that bind ubiquitin in non-overlapping 
       regions

<400>  3

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Gln Glu Val Gln Ala Trp Asp Gly Glu Val 
            20                  25                  30          


Arg Gln Val Ser Lys His Ala Phe Ser Leu Lys Gln Leu Asp Asn Pro 
        35                  40                  45              


Ala Arg Ile Pro Pro Ser Gly Trp Lys Cys Ser Lys Cys Asp Met Arg 
    50                  55                  60                  


Glu Asn Leu Trp Leu Asn Leu Thr Asp Gly Ser Ile Leu Cys Gly Arg 
65                  70                  75                  80  


Arg Tyr Phe Asp Gly Ser Gly Gly Asn Asn His Ala Val Glu His Tyr 
                85                  90                  95      


Arg Glu Thr Gly Tyr Pro Leu Ala Val Lys Leu Gly Thr Ile Thr Pro 
            100                 105                 110         


Asp Gly Ala Asp Val Tyr Ser Tyr Asp Glu Asp Asp Met Val Leu Asp 
        115                 120                 125             


Pro Ser Leu Ala Glu His Leu Ser His Phe Gly Ile Asp Met Leu Lys 
    130                 135                 140                 


Met Gln Lys Thr Gly Gly Ser Gly Gly Ser Gly Ser Ala Ala Ala Glu 
145                 150                 155                 160 


Glu Ala Glu Leu Asp Leu Lys Ala Ala Ile Gln Glu Ser Leu Arg Glu 
                165                 170                 175     


Ala Gly Gly Gly Ser Ser Gly Gly Gly Ser Asp Leu Leu Cys Lys Lys 
            180                 185                 190         


Gly Cys Gly Tyr Tyr Gly Asn Pro Ala Trp Gln Gly Phe Cys Ser Lys 
        195                 200                 205             


Cys Trp Arg Glu Glu Tyr His Lys Ala Cys Gln Lys 
    210                 215                 220 


<210>  4
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tIDR - Chimeric polypeptide comprising three binding domains that
       bind ubiquitin in non-overlapping regions

<400>  4

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His His Met Lys Gln Glu Val Gln Ala Trp Asp Gly Glu 
            20                  25                  30          


Val Arg Gln Val Ser Lys His Ala Phe Ser Leu Lys Gln Leu Asp Asn 
        35                  40                  45              


Pro Ala Arg Ile Pro Pro Ser Gly Trp Lys Cys Ser Lys Cys Asp Met 
    50                  55                  60                  


Arg Glu Asn Leu Trp Leu Asn Leu Thr Asp Gly Ser Ile Leu Cys Gly 
65                  70                  75                  80  


Arg Arg Tyr Phe Asp Gly Ser Gly Gly Asn Asn His Ala Val Glu His 
                85                  90                  95      


Tyr Arg Glu Thr Gly Tyr Pro Leu Ala Val Lys Leu Gly Thr Ile Thr 
            100                 105                 110         


Pro Asp Gly Ala Asp Val Tyr Ser Tyr Asp Glu Asp Asp Met Val Leu 
        115                 120                 125             


Asp Pro Ser Leu Ala Glu His Leu Ser His Phe Gly Ile Asp Met Leu 
    130                 135                 140                 


Lys Met Gln Lys Thr Gly Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser 
145                 150                 155                 160 


Gly Pro Pro Glu Glu Arg Tyr Glu His Gln Leu Arg Gln Leu Asn Asp 
                165                 170                 175     


Met Gly Phe Phe Asp Phe Asp Arg Asn Val Ala Ala Leu Arg Arg Ser 
            180                 185                 190         


Gly Gly Ser Val Gln Gly Ala Leu Asp Ser Leu Leu Asn Gly Gly Gly 
        195                 200                 205             


Gly Gly Ser Ser Gly Gly Gly Ser Asp Leu Leu Cys Lys Lys Gly Cys 
    210                 215                 220                 


Gly Tyr Tyr Gly Asn Pro Ala Trp Gln Gly Phe Cys Ser Lys Cys Trp 
225                 230                 235                 240 


Arg Glu Glu Tyr His Lys Ala Arg Gln Lys 
                245                 250 


<210>  5
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tIV - Atto532-tagged chimeric polypeptide comprising two binding 
       domains that bind ubiquitin in non-overlapping regions

<400>  5

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Gln Glu Val Gln Ala Trp Asp Gly Glu Val 
            20                  25                  30          


Arg Gln Val Ser Lys His Ala Phe Ser Leu Lys Gln Leu Asp Asn Pro 
        35                  40                  45              


Ala Arg Ile Pro Pro Ser Gly Trp Lys Cys Ser Lys Cys Asp Met Arg 
    50                  55                  60                  


Glu Asn Leu Trp Leu Asn Leu Thr Asp Gly Ser Ile Leu Cys Gly Arg 
65                  70                  75                  80  


Arg Tyr Phe Asp Gly Ser Gly Gly Asn Asn His Ala Val Glu His Tyr 
                85                  90                  95      


Arg Glu Thr Gly Tyr Pro Leu Ala Val Lys Leu Gly Thr Ile Thr Pro 
            100                 105                 110         


Asp Gly Ala Asp Val Trp Ser Tyr Asp Glu Asp Asp Met Val Leu Asp 
        115                 120                 125             


Pro Ser Leu Ala Glu His Leu Ser His Phe Gly Ile Asp Met Leu Lys 
    130                 135                 140                 


Met Gln Lys Gly Ser Cys Ala Ala Ala Glu Glu Ala Glu Leu Asp Leu 
145                 150                 155                 160 


Lys Ala Ala Ile Gln Glu Ser Leu Arg Glu Ala 
                165                 170     


<210>  6
<211>  208
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tISR - Chimeric polypeptide comprising three binding domains that
       bind ubiquitin in non-overlapping regions

<400>  6

Met Pro Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Gln Glu Val Gln Ala Trp Asp Gly Glu Val 
            20                  25                  30          


Arg Gln Val Ser Lys His Ala Phe Ser Leu Lys Gln Leu Asp Asn Pro 
        35                  40                  45              


Ala Arg Ile Pro Pro Ser Gly Trp Lys Cys Ser Lys Cys Asp Met Arg 
    50                  55                  60                  


Glu Asn Leu Trp Leu Asn Leu Thr Asp Gly Ser Ile Leu Cys Gly Arg 
65                  70                  75                  80  


Arg Tyr Trp Asp Gly Ser Gly Gly Asn Asn His Ala Val Glu His Tyr 
                85                  90                  95      


Arg Glu Thr Gly Tyr Pro Leu Ala Val Lys Leu Gly Thr Ile Thr Pro 
            100                 105                 110         


Asp Gly Ala Asp Val Trp Ser Tyr Asp Glu Asp Asp Met Val Leu Asp 
        115                 120                 125             


Pro Ser Leu Ala Glu His Leu Ser His Phe Gly Ile Asp Met Leu Lys 
    130                 135                 140                 


Met Gln Lys Gly Ser Cys Ala Ala Glu Glu Ala Glu Glu Gln Ile Ala 
145                 150                 155                 160 


Tyr Ala Met Gln Met Ser Leu Arg Glu Ala Gly Gly Gly Ser Asp Leu 
                165                 170                 175     


Leu Cys Lys Lys Gly Cys Gly Tyr Tyr Gly Asn Pro Ala Trp Gln Gly 
            180                 185                 190         


Phe Cys Ser Lys Cys Trp Arg Glu Glu Ala His Lys Ala Ala Gln Lys 
        195                 200                 205             


<210>  7
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tSR - chimeric polypeptide comprising two binding domains that 
       bind ubiquitin in non-overlapping regions

<400>  7

Met Pro Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Thr Glu Glu Glu Gln Ile Ala Tyr Ala Met Gln 
            20                  25                  30          


Met Ser Leu Arg Glu Ala Gly Gly Gly Ser Asp Leu Leu Cys Lys Lys 
        35                  40                  45              


Gly Cys Gly Tyr Tyr Gly Asn Pro Ala Trp Gln Gly Phe Cys Ser Lys 
    50                  55                  60                  


Cys Trp Arg Glu Glu Ala His Lys Cys Ala Ala Glu Arg Ala Ala Ala 
65                  70                  75                  80  


Glu 
    


<210>  8
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IsoT ZnF - chimeric polypeptide comprising one binding domain 
       that binds ubiquitin

<400>  8
000
<210>  9
<211>  18
<212>  PRT
<213>  Saccharomyces sp.

<400>  9

Glu Glu Ala Glu Leu Asp Leu Lys Ala Ala Ile Gln Glu Ser Leu Arg 
1               5                   10                  15      


Glu Ala 
        


<210>  10
<211>  35
<212>  PRT
<213>  Homo sapiens

<400>  10

Ser Asp Leu Leu Cys Lys Lys Gly Cys Gly Tyr Tyr Gly Asn Pro Ala 
1               5                   10                  15      


Trp Gln Gly Phe Cys Ser Lys Cys Trp Arg Glu Glu Tyr His Lys Ala 
            20                  25                  30          


Arg Gln Lys 
        35  


<210>  11
<211>  44
<212>  PRT
<213>  Saccharomyces sp.

<400>  11

Pro Pro Glu Glu Arg Tyr Glu His Gln Leu Arg Gln Leu Asn Asp Met 
1               5                   10                  15      


Gly Phe Phe Asp Phe Asp Arg Asn Val Ala Ala Leu Arg Arg Ser Gly 
            20                  25                  30          


Gly Ser Val Gln Gly Ala Leu Asp Ser Leu Leu Asn 
        35                  40                  


<210>  12
<211>  18
<212>  PRT
<213>  Homo sapiens

<400>  12

Glu Glu Ala Glu Glu Gln Ile Ala Tyr Ala Met Gln Met Ser Leu Arg 
1               5                   10                  15      


Glu Ala 
        


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  13

Gly Ser Ala Ala Ala 
1               5   


<210>  14
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  14

Gly Gly Gly 
1           


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  15

Gly Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly 
1               5                   10          


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  16

Gly Gly Gly Gly Gly Ser Ser Gly Gly Gly 
1               5                   10  


<210>  17
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  17

Gly Ser Cys Ala Ala Ala 
1               5       


<210>  18
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  18

Gly Ser Cys Ala Ala Ala 
1               5       


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  19

Gly Ser Cys Ala Ala 
1               5   


<210>  20
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  20

Gly Gly Gly 
1           


<210>  21
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  21

Gly Gly Gly 
1           


<210>  22
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  22

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  23
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  23

Met Leu Ile Lys Val Lys Thr Leu Thr Gly Lys Glu Ile Glu Ile Asp 
1               5                   10                  15      


Ile Glu Pro Thr Asp Lys Val Glu Arg Ile Lys Glu Arg Val Glu Glu 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Gln Gln Gln Arg Leu Ile Tyr Ser Gly Lys 
        35                  40                  45              


Gln Met Asn Asp Glu Lys Thr Ala Ala Asp Tyr Lys Ile Leu Gly Gly 
    50                  55                  60                  


Ser Val Leu His Leu Val Leu Ala Leu Arg Gly Gly 
65                  70                  75      


<210>  24
<211>  96
<212>  PRT
<213>  Homo sapiens

<400>  24

Ser Asp Gln Glu Ala Lys Pro Ser Thr Glu Asp Leu Gly Asp Lys Lys 
1               5                   10                  15      


Glu Gly Glu Tyr Ile Lys Leu Lys Val Ile Gly Gln Asp Ser Ser Glu 
            20                  25                  30          


Ile His Phe Lys Val Lys Met Thr Thr His Leu Lys Lys Leu Lys Glu 
        35                  40                  45              


Ser Tyr Cys Gln Arg Gln Gly Val Pro Met Asn Ser Leu Arg Phe Leu 
    50                  55                  60                  


Phe Glu Gly Gln Arg Ile Ala Asp Asn His Thr Pro Lys Glu Leu Gly 
65                  70                  75                  80  


Met Glu Glu Glu Asp Val Ile Glu Val Tyr Gln Glu Gln Thr Gly Gly 
                85                  90                  95      


<210>  25
<211>  69
<212>  PRT
<213>  Homo sapiens

<400>  25

Met Ala Asp Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asn Asp 
1               5                   10                  15      


His Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
            20                  25                  30          


Lys Ile Lys Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys 
        35                  40                  45              


Glu Arg Gln Leu Glu Met Glu Asp Glu Asp Thr Ile Asp Val Phe Gln 
    50                  55                  60                  


Gln Gln Thr Gly Gly 
65                  


<210>  26
<211>  92
<212>  PRT
<213>  Homo sapiens

<400>  26

Met Ser Glu Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asp His 
1               5                   10                  15      


Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe Lys 
            20                  25                  30          


Ile Lys Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys Glu 
        35                  40                  45              


Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly Gln 
    50                  55                  60                  


Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp Glu 
65                  70                  75                  80  


Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly 
                85                  90          


<210>  27
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified (S20C) ubiquitin

<400>  27

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Cys Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  28
<211>  12
<212>  PRT
<213>  Artificial Sequene

<220>
<223>  Peptide linker

<400>  28

Thr Gly Gly Ser Gly Gly Ser Gly Ser Ala Ala Ala 
1               5                   10          


<210>  29

<400>  29
000

<210>  30
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  30

Ala Ala Ala 
1           


<210>  31
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IsoT ZnF (S227C) - Atto532-tagged chimeric polypeptide comprising
       one ubiquitin binding domain

<400>  31

Met Pro Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Gln Glu Val Gln Ala Trp Asp Gly Glu Val 
            20                  25                  30          


Arg Gln Val Ser Lys His Ala Phe Ser Leu Lys Gln Leu Asp Asn Pro 
        35                  40                  45              


Ala Arg Ile Pro Pro Ser Gly Trp Lys Cys Ser Lys Cys Asp Met Arg 
    50                  55                  60                  


Glu Asn Leu Trp Leu Asn Leu Thr Asp Gly Ser Ile Leu Cys Gly Arg 
65                  70                  75                  80  


Arg Tyr Phe Asp Gly Cys Gly Gly Asn Asn His Ala Val Glu His Tyr 
                85                  90                  95      


Arg Glu Thr Gly Tyr Pro Leu Ala Val Lys Leu Gly Thr Ile Thr Pro 
            100                 105                 110         


Asp Gly Ala Asp Val Tyr Ser Tyr Asp Glu Asp Asp Met Val Leu Asp 
        115                 120                 125             


Pro Ser Leu Ala Glu His Leu Ser His Phe Gly Ile Asp Met Leu Lys 
    130                 135                 140                 


Met Gln Lys 
145         


<210>  32
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conjugated ubiquitin molecule

<400>  32

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr 
            20                  25                  30          


Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala 
        35                  40                  45              


Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile 
    50                  55                  60                  


Phe Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn 
65                  70                  75                  80  


Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
                85                  90                  95      


Gln Tyr Lys Leu Ala Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr 
            100                 105                 110         


Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr 
        115                 120                 125             


Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr 
    130                 135                 140                 


Lys Thr Phe Thr Val Thr 
145                 150 


<210>  33
<211>  147
<212>  PRT
<213>  Homo sapiens

<400>  33

Met Ala Leu Lys Arg Ile Asn Lys Glu Leu Ser Asp Leu Ala Arg Asp 
1               5                   10                  15      


Pro Pro Ala Gln Cys Ser Ala Gly Pro Val Gly Asp Asp Met Phe His 
            20                  25                  30          


Trp Gln Ala Thr Ile Met Gly Pro Asn Asp Ser Pro Tyr Gln Gly Gly 
        35                  40                  45              


Val Phe Phe Leu Thr Ile His Phe Pro Thr Asp Tyr Pro Phe Lys Pro 
    50                  55                  60                  


Pro Lys Val Ala Phe Thr Thr Arg Ile Tyr His Pro Asn Ile Asn Ser 
65                  70                  75                  80  


Asn Gly Ser Ile Cys Leu Asp Ile Leu Arg Ser Gln Trp Ser Pro Ala 
                85                  90                  95      


Leu Thr Ile Ser Lys Val Leu Leu Ser Ile Cys Ser Leu Leu Cys Asp 
            100                 105                 110         


Pro Asn Pro Asp Asp Pro Leu Val Pro Glu Ile Ala Arg Ile Tyr Lys 
        115                 120                 125             


Thr Asp Arg Asp Lys Tyr Asn Arg Ile Ser Arg Glu Trp Thr Gln Lys 
    130                 135                 140                 


Tyr Ala Met 
145         


<210>  34
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  34

Leu Gln Glu Glu Glu Glu Leu Gln Leu Ala Leu Ala Leu Ser Gln Ser 
1               5                   10                  15      


Glu Ala Glu Glu Lys 
            20      


<210>  35
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  35

Gln Lys Gln Ile Gln Glu Asp Trp Glu Leu Ala Glu Arg Leu Gln Arg 
1               5                   10                  15      


Glu Glu Glu Glu Ala Phe Ala Ser Ser Gln Ser 
            20                  25          


<210>  36
<211>  41
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  36

Asp His Glu Ser Lys Leu Ser Ile Leu Met Asp Met Phe Pro Ala Ile 
1               5                   10                  15      


Ser Lys Ser Lys Leu Gln Val His Leu Leu Glu Asn Asn Asn Asp Leu 
            20                  25                  30          


Asp Leu Thr Ile Gly Leu Leu Leu Lys 
        35                  40      


<210>  37
<211>  70
<212>  PRT
<213>  Homo sapiens

<400>  37

His Thr Leu Glu Glu Val Asn Asn Asn Val Arg Leu Leu Ser Glu Met 
1               5                   10                  15      


Leu Leu His Tyr Ser Gln Glu Asp Ser Ser Asp Gly Asp Arg Glu Leu 
            20                  25                  30          


Met Lys Glu Leu Phe Asp Gln Cys Glu Asn Lys Arg Arg Thr Leu Phe 
        35                  40                  45              


Lys Leu Ala Ser Glu Thr Glu Asp Asn Asp Asn Ser Leu Gly Asp Ile 
    50                  55                  60                  


Leu Gln Ala Ser Asp Asn 
65                  70  


<210>  38
<211>  135
<212>  PRT
<213>  Homo sapiens

<400>  38

Glu Thr Gly Tyr Thr Tyr Ile Leu Pro Lys Asn Val Leu Lys Lys Phe 
1               5                   10                  15      


Ile Cys Ile Ser Asp Leu Arg Ala Gln Ile Ala Gly Tyr Leu Tyr Gly 
            20                  25                  30          


Val Ser Pro Pro Asp Asn Pro Gln Val Lys Glu Ile Arg Cys Ile Val 
        35                  40                  45              


Met Val Pro Gln Trp Gly Thr His Gln Thr Val His Leu Pro Gly Gln 
    50                  55                  60                  


Leu Pro Gln His Glu Tyr Leu Lys Glu Met Glu Pro Leu Gly Trp Ile 
65                  70                  75                  80  


His Thr Gln Pro Asn Glu Ser Pro Gln Leu Ser Pro Gln Asp Val Thr 
                85                  90                  95      


Thr His Ala Lys Ile Met Ala Asp Asn Pro Ser Trp Asp Gly Glu Lys 
            100                 105                 110         


Thr Ile Ile Ile Thr Cys Ser Phe Thr Pro Gly Ser Cys Thr Leu Thr 
        115                 120                 125             


Ala Tyr Lys Leu Thr Pro Ser 
    130                 135 


<210>  39
<211>  29
<212>  PRT
<213>  Rattus norvegicus

<400>  39

Thr Ser Ala Met Trp Ala Cys Gln His Cys Thr Phe Met Asn Gln Pro 
1               5                   10                  15      


Gly Thr Gly His Cys Glu Met Cys Ser Leu Pro Arg Thr 
            20                  25                  


<210>  40
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  40

Asp Gln Val Pro Cys Glu Lys Cys Gly Ser Leu Val Pro Val Trp Asp 
1               5                   10                  15      


Met Pro Glu His Met Asp Tyr His Phe Ala Leu Glu Leu 
            20                  25                  


<210>  41
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  41

Leu Phe Tyr Asp Asp Tyr Glu Asp Gly Val Glu Glu Glu Ala Asp Ser 
1               5                   10                  15      


Cys Phe Gly 
            


<210>  42
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  42

Leu Pro Glu Gly Val Asp Gln Glu Val Phe Lys Gln Leu Pro Val Asp 
1               5                   10                  15      


Ile Gln Glu Glu Ile Leu Ser Gly Lys Ser Arg Glu Lys 
            20                  25                  


