                               SEQUENCE LISTING

<110> LIU, GAOFENG
      CHANG, MIN-JU
      GNATT, AVERELL L.
      YE, TIAN      
 
<120> ENHANCED HETEROLOGOUS PRODUCTION OF LIPOXYGENASES

<130> 3060.002.US

<140>
<141>

<150> 61/817,077
<151> 2013-04-29

<160> 7     

<170> PatentIn version 3.5

<210> 1
<211> 839
<212> PRT
<213> Glycine max

<400> 1
Met Phe Ser Ala Gly His Lys Ile Lys Gly Thr Val Val Leu Met Pro 
1               5                   10                  15      


Lys Asn Glu Leu Glu Val Asn Pro Asp Gly Ser Ala Val Asp Asn Leu 
            20                  25                  30          


Asn Ala Phe Leu Gly Arg Ser Val Ser Leu Gln Leu Ile Ser Ala Thr 
        35                  40                  45              


Lys Ala Asp Ala His Gly Lys Gly Lys Val Gly Lys Asp Thr Phe Leu 
    50                  55                  60                  


Glu Gly Ile Asn Thr Ser Leu Pro Thr Leu Gly Ala Gly Glu Ser Ala 
65                  70                  75                  80  


Phe Asn Ile His Phe Glu Trp Asp Gly Ser Met Gly Ile Pro Gly Ala 
                85                  90                  95      


Phe Tyr Ile Lys Asn Tyr Met Gln Val Glu Phe Phe Leu Lys Ser Leu 
            100                 105                 110         


Thr Leu Glu Ala Ile Ser Asn Gln Gly Thr Ile Arg Phe Val Cys Asn 
        115                 120                 125             


Ser Trp Val Tyr Asn Thr Lys Leu Tyr Lys Ser Val Arg Ile Phe Phe 
    130                 135                 140                 


Ala Asn His Thr Tyr Val Pro Ser Glu Thr Pro Ala Pro Leu Val Ser 
145                 150                 155                 160 


Tyr Arg Glu Glu Glu Leu Lys Ser Leu Arg Gly Asn Gly Thr Gly Glu 
                165                 170                 175     


Arg Lys Glu Tyr Asp Arg Ile Tyr Asp Tyr Asp Val Tyr Asn Asp Leu 
            180                 185                 190         


Gly Asn Pro Asp Lys Ser Glu Lys Leu Ala Arg Pro Val Leu Gly Gly 
        195                 200                 205             


Ser Ser Thr Phe Pro Tyr Pro Arg Arg Gly Arg Thr Gly Arg Gly Pro 
    210                 215                 220                 


Thr Val Thr Asp Pro Asn Thr Glu Lys Gln Gly Glu Val Phe Tyr Val 
225                 230                 235                 240 


Pro Arg Asp Glu Asn Leu Gly His Leu Lys Ser Lys Asp Ala Leu Glu 
                245                 250                 255     


Ile Gly Thr Lys Ser Leu Ser Gln Ile Val Gln Pro Ala Phe Glu Ser 
            260                 265                 270         


Ala Phe Asp Leu Lys Ser Thr Pro Ile Glu Phe His Ser Phe Gln Asp 
        275                 280                 285             


Val His Asp Leu Tyr Glu Gly Gly Ile Lys Leu Pro Arg Asp Val Ile 
    290                 295                 300                 


Ser Thr Ile Ile Pro Leu Pro Val Ile Lys Glu Leu Tyr Arg Thr Asp 
305                 310                 315                 320 


Gly Gln His Ile Leu Lys Phe Pro Gln Pro His Val Val Gln Val Ser 
                325                 330                 335     


Gln Ser Ala Trp Met Thr Asp Glu Glu Phe Ala Arg Glu Met Ile Ala 
            340                 345                 350         


Gly Val Asn Pro Cys Val Ile Arg Gly Leu Glu Glu Phe Pro Pro Lys 
        355                 360                 365             


Ser Asn Leu Asp Pro Ala Ile Tyr Gly Asp Gln Ser Ser Lys Ile Thr 
    370                 375                 380                 


Ala Asp Ser Leu Asp Leu Asp Gly Tyr Thr Met Asp Glu Ala Leu Gly 
385                 390                 395                 400 


Ser Arg Arg Leu Phe Met Leu Asp Tyr His Asp Ile Phe Met Pro Tyr 
                405                 410                 415     


Val Arg Gln Ile Asn Gln Leu Asn Ser Ala Lys Thr Tyr Ala Thr Arg 
            420                 425                 430         


Thr Ile Leu Phe Leu Arg Glu Asp Gly Thr Leu Lys Pro Val Ala Ile 
        435                 440                 445             


Glu Leu Ser Leu Pro His Ser Ala Gly Asp Leu Ser Ala Ala Val Ser 
    450                 455                 460                 


Gln Val Val Leu Pro Ala Lys Glu Gly Val Glu Ser Thr Ile Trp Leu 
465                 470                 475                 480 


Leu Ala Lys Ala Tyr Val Ile Val Asn Asp Ser Cys Tyr His Gln Leu 
                485                 490                 495     


Met Ser His Trp Leu Asn Thr His Ala Ala Met Glu Pro Phe Val Ile 
            500                 505                 510         


Ala Thr His Arg His Leu Ser Val Leu His Pro Ile Tyr Lys Leu Leu 
        515                 520                 525             


Thr Pro His Tyr Arg Asn Asn Met Asn Ile Asn Ala Leu Ala Arg Gln 
    530                 535                 540                 


Ser Leu Ile Asn Ala Asn Gly Ile Ile Glu Thr Thr Phe Leu Pro Ser 
545                 550                 555                 560 


Lys Tyr Ser Val Glu Met Ser Ser Ala Val Tyr Lys Asn Trp Val Phe 
                565                 570                 575     


Thr Asp Gln Ala Leu Pro Ala Asp Leu Ile Lys Arg Gly Val Ala Ile 
            580                 585                 590         


Lys Asp Pro Ser Thr Pro His Gly Val Arg Leu Leu Ile Glu Asp Tyr 
        595                 600                 605             


Pro Tyr Ala Ala Asp Gly Leu Glu Ile Trp Ala Ala Ile Lys Thr Trp 
    610                 615                 620                 


Val Gln Glu Tyr Val Pro Leu Tyr Tyr Ala Arg Asp Asp Asp Val Lys 
625                 630                 635                 640 


Asn Asp Ser Glu Leu Gln His Trp Trp Lys Glu Ala Val Glu Lys Gly 
                645                 650                 655     


His Gly Asp Leu Lys Asp Lys Pro Trp Trp Pro Lys Leu Gln Thr Leu 
            660                 665                 670         


Glu Asp Leu Val Glu Val Cys Leu Ile Ile Ile Trp Ile Ala Ser Ala 
        675                 680                 685             


Leu His Ala Ala Val Asn Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile 
    690                 695                 700                 


Met Asn Arg Pro Thr Ala Ser Arg Arg Leu Leu Pro Glu Lys Gly Thr 
705                 710                 715                 720 


Pro Glu Tyr Glu Glu Met Ile Asn Asn His Glu Lys Ala Tyr Leu Arg 
                725                 730                 735     


Thr Ile Thr Ser Lys Leu Pro Thr Leu Ile Ser Leu Ser Val Ile Glu 
            740                 745                 750         


Ile Leu Ser Thr His Ala Ser Asp Glu Val Tyr Leu Gly Gln Arg Asp 
        755                 760                 765             


Asn Pro His Trp Thr Ser Asp Ser Lys Ala Leu Gln Ala Phe Gln Lys 
    770                 775                 780                 


Phe Gly Asn Lys Leu Lys Glu Ile Glu Glu Lys Leu Val Arg Arg Asn 
785                 790                 795                 800 


Asn Asp Pro Ser Leu Gln Gly Asn Arg Leu Gly Pro Val Gln Leu Pro 
                805                 810                 815     


Tyr Thr Leu Leu Tyr Pro Ser Ser Glu Glu Gly Leu Thr Phe Arg Gly 
            820                 825                 830         


Ile Pro Asn Ser Ile Ser Ile 
        835                 


<210> 2
<211> 857
<212> PRT
<213> Glycine max

<400> 2
Met Leu Gly Gly Leu Leu His Arg Gly His Lys Ile Lys Gly Thr Val 
1               5                   10                  15      


Val Leu Met Arg Lys Asn Val Leu Asp Val Asn Ser Val Thr Ser Val 
            20                  25                  30          


Gly Gly Ile Ile Gly Gln Gly Leu Asp Leu Val Gly Ser Thr Leu Asp 
        35                  40                  45              


Thr Leu Thr Ala Phe Leu Gly Arg Ser Val Ser Leu Gln Leu Ile Ser 
    50                  55                  60                  


Ala Thr Lys Ala Asp Ala Asn Gly Lys Gly Lys Leu Gly Lys Ala Thr 
65                  70                  75                  80  


Phe Leu Glu Gly Ile Ile Thr Ser Leu Pro Thr Leu Gly Ala Gly Gln 
                85                  90                  95      


Ser Ala Phe Lys Ile Asn Phe Glu Trp Asp Asp Gly Ser Gly Ile Pro 
            100                 105                 110         


Gly Ala Phe Tyr Ile Lys Asn Phe Met Gln Thr Glu Phe Phe Leu Val 
        115                 120                 125             


Ser Leu Thr Leu Glu Asp Ile Pro Asn His Gly Ser Ile His Phe Val 
    130                 135                 140                 


Cys Asn Ser Trp Ile Tyr Asn Ala Lys Leu Phe Lys Ser Asp Arg Ile 
145                 150                 155                 160 


Phe Phe Ala Asn Gln Thr Tyr Leu Pro Ser Glu Thr Pro Ala Pro Leu 
                165                 170                 175     


Val Lys Tyr Arg Glu Glu Glu Leu His Asn Leu Arg Gly Asp Gly Thr 
            180                 185                 190         


Gly Glu Arg Lys Glu Trp Glu Arg Ile Tyr Asp Tyr Asp Val Tyr Asn 
        195                 200                 205             


Asp Leu Gly Asp Pro Asp Lys Gly Glu Asn His Ala Arg Pro Val Leu 
    210                 215                 220                 


Gly Gly Asn Asp Thr Phe Pro Tyr Pro Arg Arg Gly Arg Thr Gly Arg 
225                 230                 235                 240 


Lys Pro Thr Arg Lys Asp Pro Asn Ser Glu Ser Arg Ser Asn Asp Val 
                245                 250                 255     


Tyr Leu Pro Arg Asp Glu Ala Phe Gly His Leu Lys Ser Ser Asp Phe 
            260                 265                 270         


Leu Thr Tyr Gly Leu Lys Ser Val Ser Gln Asn Val Leu Pro Leu Leu 
        275                 280                 285             


Gln Ser Ala Phe Asp Leu Asn Phe Thr Pro Arg Glu Phe Asp Ser Phe 
    290                 295                 300                 


Asp Glu Val His Gly Leu Tyr Ser Gly Gly Ile Lys Leu Pro Thr Asp 
305                 310                 315                 320 


Ile Ile Ser Lys Ile Ser Pro Leu Pro Val Leu Lys Glu Ile Phe Arg 
                325                 330                 335     


Thr Asp Gly Glu Gln Ala Leu Lys Phe Pro Pro Pro Lys Val Ile Gln 
            340                 345                 350         


Val Ser Lys Ser Ala Trp Met Thr Asp Glu Glu Phe Ala Arg Glu Met 
        355                 360                 365             


Leu Ala Gly Val Asn Pro Asn Leu Ile Arg Cys Leu Lys Asp Phe Pro 
    370                 375                 380                 


Pro Arg Ser Lys Leu Asp Ser Gln Val Tyr Gly Asp His Thr Ser Gln 
385                 390                 395                 400 


Ile Thr Lys Glu His Leu Glu Pro Asn Leu Glu Gly Leu Thr Val Asp 
                405                 410                 415     


Glu Ala Ile Gln Asn Lys Arg Leu Phe Leu Leu Asp His His Asp Pro 
            420                 425                 430         


Ile Met Pro Tyr Leu Arg Arg Ile Asn Ala Thr Ser Thr Lys Ala Tyr 
        435                 440                 445             


Ala Thr Arg Thr Ile Leu Phe Leu Lys Asn Asp Gly Thr Leu Arg Pro 
    450                 455                 460                 


Leu Ala Ile Glu Leu Ser Leu Pro His Pro Gln Gly Asp Gln Ser Gly 
465                 470                 475                 480 


Ala Phe Ser Gln Val Phe Leu Pro Ala Asp Glu Gly Val Glu Ser Ser 
                485                 490                 495     


Ile Trp Leu Leu Ala Lys Ala Tyr Val Val Val Asn Asp Ser Cys Tyr 
            500                 505                 510         


His Gln Leu Val Ser His Trp Leu Asn Thr His Ala Val Val Glu Pro 
        515                 520                 525             


Phe Ile Ile Ala Thr Asn Arg His Leu Ser Val Val His Pro Ile Tyr 
    530                 535                 540                 


Lys Leu Leu His Pro His Tyr Arg Asp Thr Met Asn Ile Asn Gly Leu 
545                 550                 555                 560 


Ala Arg Leu Ser Leu Val Asn Asp Gly Gly Val Ile Glu Gln Thr Phe 
                565                 570                 575     


Leu Trp Gly Arg Tyr Ser Val Glu Met Ser Ala Val Val Tyr Lys Asp 
            580                 585                 590         


Trp Val Phe Thr Asp Gln Ala Leu Pro Ala Asp Leu Ile Lys Arg Gly 
        595                 600                 605             


Met Ala Ile Glu Asp Pro Ser Cys Pro His Gly Ile Arg Leu Val Ile 
    610                 615                 620                 


Glu Asp Tyr Pro Tyr Thr Val Asp Gly Leu Glu Ile Trp Asp Ala Ile 
625                 630                 635                 640 


Lys Thr Trp Val His Glu Tyr Val Phe Leu Tyr Tyr Lys Ser Asp Asp 
                645                 650                 655     


Thr Leu Arg Glu Asp Pro Glu Leu Gln Ala Cys Trp Lys Glu Leu Val 
            660                 665                 670         


Glu Val Gly His Gly Asp Lys Lys Asn Glu Pro Trp Trp Pro Lys Met 
        675                 680                 685             


Gln Thr Arg Glu Glu Leu Val Glu Ala Cys Ala Ile Ile Ile Trp Thr 
    690                 695                 700                 


Ala Ser Ala Leu His Ala Ala Val Asn Phe Gly Gln Tyr Pro Tyr Gly 
705                 710                 715                 720 


Gly Leu Ile Leu Asn Arg Pro Thr Leu Ser Arg Arg Phe Met Pro Glu 
                725                 730                 735     


Lys Gly Ser Ala Glu Tyr Glu Glu Leu Arg Lys Asn Pro Gln Lys Ala 
            740                 745                 750         


Tyr Leu Lys Thr Ile Thr Pro Lys Phe Gln Thr Leu Ile Asp Leu Ser 
        755                 760                 765             


Val Ile Glu Ile Leu Ser Arg His Ala Ser Asp Glu Val Tyr Leu Gly 
    770                 775                 780                 


Glu Arg Asp Asn Pro Asn Trp Thr Ser Asp Thr Arg Ala Leu Glu Ala 
785                 790                 795                 800 


Phe Lys Arg Phe Gly Asn Lys Leu Ala Gln Ile Glu Asn Lys Leu Ser 
                805                 810                 815     


Glu Arg Asn Asn Asp Glu Lys Leu Arg Asn Arg Cys Gly Pro Val Gln 
            820                 825                 830         


Met Pro Tyr Thr Leu Leu Leu Pro Ser Ser Lys Glu Gly Leu Thr Phe 
        835                 840                 845             


Arg Gly Ile Pro Asn Ser Ile Ser Ile 
    850                 855         


<210> 3
<211> 563
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Ser Thr Pro Ile Glu Phe His Ser Phe Gln Asp Val His Asp Leu 
1               5                   10                  15      


Tyr Glu Gly Gly Ile Lys Leu Pro Arg Asp Val Ile Ser Thr Ile Ile 
            20                  25                  30          


Pro Leu Pro Val Ile Lys Glu Leu Tyr Arg Thr Asp Gly Gln His Ile 
        35                  40                  45              


Leu Lys Phe Pro Gln Pro His Val Val Gln Val Ser Gln Ser Ala Trp 
    50                  55                  60                  


Met Thr Asp Glu Glu Phe Ala Arg Glu Met Ile Ala Gly Val Asn Pro 
65                  70                  75                  80  


Cys Val Ile Arg Gly Leu Glu Glu Phe Pro Pro Lys Ser Asn Leu Asp 
                85                  90                  95      


Pro Ala Ile Tyr Gly Asp Gln Ser Ser Lys Ile Thr Ala Asp Ser Leu 
            100                 105                 110         


Asp Leu Asp Gly Tyr Thr Met Asp Glu Ala Leu Gly Ser Arg Arg Leu 
        115                 120                 125             


Phe Met Leu Asp Tyr His Asp Ile Phe Met Pro Tyr Val Arg Gln Ile 
    130                 135                 140                 


Asn Gln Leu Asn Ser Ala Lys Thr Tyr Ala Thr Arg Thr Ile Leu Phe 
145                 150                 155                 160 


Leu Arg Glu Asp Gly Thr Leu Lys Pro Val Ala Ile Glu Leu Ser Leu 
                165                 170                 175     


Pro His Ser Ala Gly Asp Leu Ser Ala Ala Val Ser Gln Val Val Leu 
            180                 185                 190         


Pro Ala Lys Glu Gly Val Glu Ser Thr Ile Trp Leu Leu Ala Lys Ala 
        195                 200                 205             


Tyr Val Ile Val Asn Asp Ser Cys Tyr His Gln Leu Met Ser His Trp 
    210                 215                 220                 


Leu Asn Thr His Ala Ala Met Glu Pro Phe Val Ile Ala Thr His Arg 
225                 230                 235                 240 


His Leu Ser Val Leu His Pro Ile Tyr Lys Leu Leu Thr Pro His Tyr 
                245                 250                 255     


Arg Asn Asn Met Asn Ile Asn Ala Leu Ala Arg Gln Ser Leu Ile Asn 
            260                 265                 270         


Ala Asn Gly Ile Ile Glu Thr Thr Phe Leu Pro Ser Lys Tyr Ser Val 
        275                 280                 285             


Glu Met Ser Ser Ala Val Tyr Lys Asn Trp Val Phe Thr Asp Gln Ala 
    290                 295                 300                 


Leu Pro Ala Asp Leu Ile Lys Arg Gly Val Ala Ile Lys Asp Pro Ser 
305                 310                 315                 320 


Thr Pro His Gly Val Arg Leu Leu Ile Glu Asp Tyr Pro Tyr Ala Ala 
                325                 330                 335     


Asp Gly Leu Glu Ile Trp Ala Ala Ile Lys Thr Trp Val Gln Glu Tyr 
            340                 345                 350         


Val Pro Leu Tyr Tyr Ala Arg Asp Asp Asp Val Lys Asn Asp Ser Glu 
        355                 360                 365             


Leu Gln His Trp Trp Lys Glu Ala Val Glu Lys Gly His Gly Asp Leu 
    370                 375                 380                 


Lys Asp Lys Pro Trp Trp Pro Lys Leu Gln Thr Leu Glu Asp Leu Val 
385                 390                 395                 400 


Glu Val Cys Leu Ile Ile Ile Trp Ile Ala Ser Ala Leu His Ala Ala 
                405                 410                 415     


Val Asn Phe Gly Gln Tyr Pro Tyr Gly Gly Leu Ile Met Asn Arg Pro 
            420                 425                 430         


Thr Ala Ser Arg Arg Leu Leu Pro Glu Lys Gly Thr Pro Glu Tyr Glu 
        435                 440                 445             


Glu Met Ile Asn Asn His Glu Lys Ala Tyr Leu Arg Thr Ile Thr Ser 
    450                 455                 460                 


Lys Leu Pro Thr Leu Ile Ser Leu Ser Val Ile Glu Ile Leu Ser Thr 
465                 470                 475                 480 


His Ala Ser Asp Glu Val Tyr Leu Gly Gln Arg Asp Asn Pro His Trp 
                485                 490                 495     


Thr Ser Asp Ser Lys Ala Leu Gln Ala Phe Gln Lys Phe Gly Asn Lys 
            500                 505                 510         


Leu Lys Glu Ile Glu Glu Lys Leu Val Arg Arg Asn Asn Asp Pro Ser 
        515                 520                 525             


Leu Gln Gly Asn Arg Leu Gly Pro Val Gln Leu Pro Tyr Thr Leu Leu 
    530                 535                 540                 


Tyr Pro Ser Ser Glu Glu Gly Leu Thr Phe Arg Gly Ile Pro Asn Ser 
545                 550                 555                 560 


Ile Ser Ile 
            


<210> 4
<211> 2532
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 4
cccgggatgt ttagtgctgg tcacaaaatc aaaggtaccg tggtcctgat gccgaaaaat       60

gaactggaag tcaacccgga tggtagcgcc gttgataacc tgaatgcgtt cctgggtcgt      120

agcgtgtctc tgcagctgat ttccgccacc aaagcagacg ctcacggcaa gggtaaagtt      180

ggcaaagata cgtttctgga aggtattaat acctccctgc cgaccctggg tgccggtgaa      240

tcagctttca acatccattt cgaatgggat ggttcaatgg gcattccggg cgccttctac      300

atcaaaaact acatgcaggt ggaatttttc ctgaaaagtc tgaccctgga agcaatctcc      360

aatcagggta cgattcgttt tgtctgcaac tcgtgggtgt ataataccaa actgtacaaa      420

agcgttcgca tctttttcgc gaaccacacc tatgttccga gcgaaacccc ggcaccgctg      480

gtttcttacc gtgaagaaga actgaaaagt ctgcgcggca atggtaccgg cgaacgtaaa      540

gaatatgatc gcatttatga ctacgatgtt tacaacgacc tgggcaatcc ggataaaagc      600

gaaaaactgg cccgtccggt cctgggcggt agctctacct tcccgtatcc gcgtcgcggt      660

cgtaccggtc gtggtccgac cgtgaccgat ccgaacaccg aaaaacaggg cgaagtcttt      720

tatgtgccgc gcgacgaaaa tctgggccat ctgaaatcta aagatgccct ggaaatcggt      780

accaaaagtc tgtcccagat tgtgcaaccg gcgtttgaaa gcgccttcga tctgaaatct      840

acgccgattg aatttcactc cttccaggac gttcatgatc tgtatgaagg cggtatcaaa      900

ctgccgcgtg acgtcatttc aaccattatc ccgctgccgg tgatcaaaga actgtaccgc      960

acggatggtc agcacattct gaaatttccg caaccgcatg tggttcaggt ttcacaatcg     1020

gcgtggatga ccgatgaaga attcgcgcgt gaaatgatcg ccggcgttaa cccgtgcgtc     1080

attcgcggtc tggaagaatt tccgccgaaa agcaatctgg acccggcaat ctatggcgat     1140

cagagttcca aaattaccgc tgactctctg gacctggatg gctacacgat ggatgaagcc     1200

ctgggtagtc gtcgcctgtt tatgctggac tatcacgata tcttcatgcc gtacgtgcgt     1260

cagattaacc aactgaattc tgcaaaaacc tatgctaccc gtacgatcct gtttctgcgc     1320

gaagacggca cgctgaaacc ggttgcaatt gaactgagcc tgccgcattc tgctggtgat     1380

ctgagtgccg cggtgtccca ggttgtgctg ccggcaaaag aaggcgttga aagtaccatc     1440

tggctgctgg cgaaagccta tgttattgtc aacgattcat gttaccatca actgatgtcg     1500

cactggctga atacccatgc agctatggaa ccgtttgtta tcgcaacgca tcgccacctg     1560

tctgtcctgc acccgattta taaactgctg accccgcatt accgtaacaa tatgaacatc     1620

aatgcactgg ctcgccagag tctgattaac gcgaatggta ttatcgaaac cacgttcctg     1680

ccgtcaaaat attcggtgga aatgtcatcg gccgtttaca aaaactgggt ctttaccgac     1740

caggcactgc cggctgatct gatcaaacgt ggcgtcgcga ttaaagatcc gagcaccccg     1800

catggtgtgc gtctgctgat tgaagactat ccgtacgcgg ccgatggcct ggaaatctgg     1860

gcagctatta aaacctgggt gcaggaatat gttccgctgt attacgcacg cgatgacgat     1920

gtgaaaaatg actccgaact gcaacactgg tggaaagaag ctgttgaaaa aggtcatggc     1980

gacctgaaag ataaaccgtg gtggccgaaa ctgcagaccc tggaagatct ggtggaagtt     2040

tgtctgatta tcatttggat tgccagcgca ctgcatgccg cggtgaactt tggtcaatat     2100

ccgtacggcg gtctgattat gaatcgtccg accgcaagcc gtcgcctgct gccggaaaaa     2160

ggcacgccgg aatacgaaga aatgatcaac aaccatgaaa aagcgtacct gcgcaccatc     2220

acgagcaaac tgccgaccct gattagcctg tctgttatcg aaattctgtc aacgcacgcg     2280

tcggatgaag tctatctggg tcagcgtgac aacccgcatt ggaccagtga ttccaaagcg     2340

ctgcaggcct tccaaaaatt cggcaacaaa ctgaaagaaa tcgaagaaaa actggtccgt     2400

cgcaacaatg atccgagcct gcagggtaac cgtctgggtc cggtgcaact gccgtatacc     2460

ctgctgtatc cgtccagtga agaaggtctg acgtttcgtg gtattccgaa ctccatttcc     2520

atctgactcg ag                                                         2532


<210> 5
<211> 2583
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 5
catatgctgg gcggcctgct gcaccgtggt cataaaatca agggcaccgt ggtcctgatg       60

cgtaagaacg tcctggatgt gaatagcgtg acctcggtcg gcggtattat cggccagggt      120

ctggacctgg tgggtagcac gctggatacc ctgacggcct ttctgggccg ctcagtgtcg      180

ctgcaactga tcagcgcaac caaagcagat gctaacggca aaggcaagct gggcaaggcg      240

acgttcctgg aaggcattat cacctccctg ccgacgctgg gtgcaggcca gtcagccttt      300

aaaattaatt tcgaatggga tgacggctct ggtattccgg gcgccttcta catcaagaac      360

ttcatgcaga ccgaattttt cctggtcagc ctgacgctgg aagatatccc gaatcatggc      420

tcgattcact ttgtgtgcaa cagctggatc tacaatgcga aactgttcaa gtccgatcgc      480

attttctttg ccaatcagac ctatctgccg tcagaaacgc cggcaccgct ggttaaatac      540

cgtgaagaag aactgcacaa cctgcgtggt gacggtaccg gtgaacgtaa agaatgggaa      600

cgcatctacg attacgacgt ttacaacgat ctgggtgatc cggacaaagg cgaaaaccat      660

gcgcgtccgg tcctgggcgg taatgacacc tttccgtacc cgcgtcgcgg tcgtaccggt      720

cgtaaaccga cgcgtaagga tccgaacagc gaatctcgca gtaatgatgt gtatctgccg      780

cgtgacgaag cctttggtca cctgaaaagc tctgatttcc tgacgtacgg cctgaagtcc      840

gtttcacaga acgtcctgcc gctgctgcaa agcgcatttg atctgaattt caccccgcgc      900

gaatttgatt cgttcgacga agttcatggt ctgtatagcg gcggtattaa gctgccgacc      960

gacattatct ctaaaatcag tccgctgccg gtgctgaagg aaatttttcg cacggatggc     1020

gaacaggctc tgaagttccc gccgccgaaa gtcatccaag tgtcgaaaag cgcgtggatg     1080

accgatgaag aatttgcacg tgaaatgctg gctggtgtta acccgaatct gattcgctgt     1140

ctgaaggatt tcccgccgcg ttccaaactg gattcacagg tgtatggtga ccacaccagt     1200

caaatcacga aagaacatct ggaaccgaac ctggaaggcc tgaccgttga tgaagctatt     1260

cagaataaac gtctgtttct gctggatcat cacgacccga tcatgccgta tctgcgtcgc     1320

attaatgcga cctcgacgaa agcgtacgcc acccgcacga tcctgttcct gaagaacgat     1380

ggtaccctgc gtccgctggc cattgaactg agcctgccgc atccgcaggg tgaccaatcg     1440

ggtgcgttta gccaggtttt cctgccggcc gatgaaggcg tcgaaagttc catctggctg     1500

ctggcaaaag cttatgtggt tgtcaacgat tcttgctacc atcagctggt gtctcactgg     1560

ctgaataccc atgcagtggt tgaaccgttt attatcgcta cgaaccgcca cctgtctgtc     1620

gtgcatccga tctataaact gctgcatccg cactaccgcg acaccatgaa cattaatggt     1680

ctggcgcgtc tgagtctggt caacgatggc ggtgtgattg aacagacgtt tctgtggggc     1740

cgttattctg ttgaaatgag tgccgttgtc tacaaagatt gggtcttcac cgaccaagca     1800

ctgccggcag acctgatcaa gcgtggtatg gcaattgaag atccgtcctg tccgcacggc     1860

atccgtctgg tgattgaaga ttatccgtac accgttgacg gtctggaaat ctgggatgca     1920

attaaaacgt gggtgcatga atacgttttt ctgtactaca agtctgatga caccctgcgc     1980

gaagacccgg aactgcaggc gtgctggaaa gaactggtgg aagttggtca cggcgataaa     2040

aagaacgaac cgtggtggcc gaaaatgcaa acccgtgaag aactggttga agcgtgtgcc     2100

attatcattt ggacggcaag cgctctgcat gcggccgtga actttggcca gtatccgtac     2160

ggcggtctga ttctgaatcg cccgaccctg tctcgtcgct tcatgccgga aaaaggcagt     2220

gctgaatatg aagaactgcg taaaaatccg cagaaggcgt acctgaaaac catcacgccg     2280

aaatttcaaa ccctgattga cctgagcgtg atcgaaattc tgtcccgcca tgcgtcagat     2340

gaagtttatc tgggtgaacg tgacaacccg aattggacct ccgatacgcg tgcactggaa     2400

gcttttaagc gcttcggcaa caaactggcc cagatcgaaa acaagctgtc agaacgtaac     2460

aacgatgaaa agctgcgtaa tcgctgcggc ccggtgcaaa tgccgtatac cctgctgctg     2520

ccgtcctcaa aagaaggtct gacgttccgt ggtatcccga atagcattag catctaagaa     2580

ttc                                                                   2583


<210> 6
<211> 1701
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 6
catatgtcta cgccgattga atttcactcc ttccaggacg ttcatgatct gtatgaaggc       60

ggtatcaaac tgccgcgtga cgtcatttca accattatcc cgctgccggt gatcaaagaa      120

ctgtaccgca cggatggtca gcacattctg aaatttccgc aaccgcatgt ggttcaggtt      180

tcacaatcgg cgtggatgac cgatgaagaa ttcgcgcgtg aaatgatcgc cggcgttaac      240

ccgtgcgtca ttcgcggtct ggaagaattt ccgccgaaaa gcaatctgga cccggcaatc      300

tatggcgatc agagttccaa aattaccgct gactctctgg acctggatgg ctacacgatg      360

gatgaagccc tgggtagtcg tcgcctgttt atgctggact atcacgatat cttcatgccg      420

tacgtgcgtc agattaacca actgaattct gcaaaaacct atgctacccg tacgatcctg      480

tttctgcgcg aagacggcac gctgaaaccg gttgcaattg aactgagcct gccgcattct      540

gctggtgatc tgagtgccgc ggtgtcccag gttgtgctgc cggcaaaaga aggcgttgaa      600

agtaccatct ggctgctggc gaaagcctat gttattgtca acgattcatg ttaccatcaa      660

ctgatgtcgc actggctgaa tacccatgca gctatggaac cgtttgttat cgcaacgcat      720

cgccacctgt ctgtcctgca cccgatttat aaactgctga ccccgcatta ccgtaacaat      780

atgaacatca atgcactggc tcgccagagt ctgattaacg cgaatggtat tatcgaaacc      840

acgttcctgc cgtcaaaata ttcggtggaa atgtcatcgg ccgtttacaa aaactgggtc      900

tttaccgacc aggcactgcc ggctgatctg atcaaacgtg gcgtcgcgat taaagatccg      960

agcaccccgc atggtgtgcg tctgctgatt gaagactatc cgtacgcggc cgatggcctg     1020

gaaatctggg cagctattaa aacctgggtg caggaatatg ttccgctgta ttacgcacgc     1080

gatgacgatg tgaaaaatga ctccgaactg caacactggt ggaaagaagc tgttgaaaaa     1140

ggtcatggcg acctgaaaga taaaccgtgg tggccgaaac tgcagaccct ggaagatctg     1200

gtggaagttt gtctgattat catttggatt gccagcgcac tgcatgccgc ggtgaacttt     1260

ggtcaatatc cgtacggcgg tctgattatg aatcgtccga ccgcaagccg tcgcctgctg     1320

ccggaaaaag gcacgccgga atacgaagaa atgatcaaca accatgaaaa agcgtacctg     1380

cgcaccatca cgagcaaact gccgaccctg attagcctgt ctgttatcga aattctgtca     1440

acgcacgcgt cggatgaagt ctatctgggt cagcgtgaca acccgcattg gaccagtgat     1500

tccaaagcgc tgcaggcctt ccaaaaattc ggcaacaaac tgaaagaaat cgaagaaaaa     1560

ctggtccgtc gcaacaatga tccgagcctg cagggtaacc gtctgggtcc ggtgcaactg     1620

ccgtataccc tgctgtatcc gtccagtgaa gaaggtctga cgtttcgtgg tattccgaac     1680

tccatttcca tctgactcga g                                               1701


<210> 7
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 7
His His His His His His 
1               5       


